Status
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Gene information
General information
TC009822 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8634026..8639531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009822
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02856

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009822 RA
      MSSSAKMVY NIDWIIPNL RNPTKLWSL ASTITVAAV 
      GLFSKIFIQ WFNKTKIHN RHVISSVLD NRPRNVPLI 
      TVSNHHSCF DDPGIWGTL KWRHLMNPK VMRWSLAAH 
      DICFTCSQH SYFFSLGKC IPVIRGAGV YQDAVDFCI 
      EQLAKGSWV HVFPEGKVN MTKENMRLK WGVGRMIFE 
      SPVTPIVVP IWHMGMDDV LPNEPPYVL RLGKNLTFN 
      YGNPIDLTE MVRKLKETN ATDVEARKQ ITDFLQEQL 
      LKLKIETEE LHKRDFKVN L
    CDS sequence
    • >TC009822 RA
      atgtcatct agtgcgaaa atggtctac aacattgac 
      tggattatc ccaaatctg cggaacccc acgaaactg 
      tggagtctg gctagcacg ataacggtg gctgctgtg 
      gggctcttc agcaagatt ttcatccag tggttcaac 
      aaaacgaag attcacaac aggcatgtt atctcttcc 
      gtgttggat aacaggccg cgaaatgtg cccctcatc 
      actgtctct aaccaccac tcatgcttt gacgacccg 
      ggcatttgg ggtacccta aagtggcgc cacctaatg 
      aaccccaaa gtgatgcgt tggtctctc gccgctcac 
      gatatctgc ttcacctgt tcccaacac tcgtatttc 
      ttcagtttg ggcaaatgc attccggtg atacgtggc 
      gccggcgtt tatcaagac gctgtcgat ttctgcatc 
      gagcaattg gcgaagggt tcatgggtt cacgttttt 
      cccgaaggc aaggtgaac atgactaag gaaaacatg 
      agactgaaa tggggcgtg gggcggatg attttcgag 
      tcgccggtg acaccgatc gtcgtaccc atctggcat 
      atgggtatg gacgacgtc ttgcccaac gaacccccg 
      tacgtcctg cggctgggc aagaatttg acgtttaat 
      tatgggaat cccatagac ttgacggag atggtgcgc 
      aagttgaaa gagacgaac gcgacggac gtggaggcg 
      aggaagcaa ataaccgac tttttgcaa gagcagttg 
      ctaaagctt aagattgaa accgaagag ttgcataag 
      cgggatttt aaagtgaat ttatga
    mRNA sequence
    • >TC009822 RA
      tgacgtctt caaatgtca caattacgt ccaatgtca 
      accatccca agtgaaatg ttcattcac cgtgtgttc 
      taatatcag ttcgtaaca atcagctat tatataatt 
      agtgcacgc cgcgtgaat cagttgcaa taaaaatca 
      gttcaattg aatgatgtg cgtccgtct gtgcacacc 
      tctcccacc gttacactc actctctta ataactcgc 
      gaattacat ctgcaataa catataatg acgatatgt 
      taaacgaat aagcatgtc atctagtgc gaaaatggt 
      ctacaacat tgactggat tatcccaaa tctgcggaa 
      ccccacgaa actgtggag tctggctag cacgataac 
      ggtggctgc tgtggggct cttcagcaa gattttcat 
      ccagtggtt caacaaaac gaagattca caacaggca 
      tgttatctc ttccgtgtt ggataacag gccgcgaaa 
      tgtgcccct catcactgt ctctaacca ccactcatg 
      ctttgacga cccgggcat ttggggtac cctaaagtg 
      gcgccacct aatgaaccc caaagtgat gcgttggtc 
      tctcgccgc tcacgatat ctgcttcac ctgttccca 
      acactcgta tttcttcag tttgggcaa atgcattcc 
      ggtgatacg tggcgccgg cgtttatca agacgctgt 
      cgatttctg catcgagca attggcgaa gggttcatg 
      ggttcacgt ttttcccga aggcaaggt gaacatgac 
      taaggaaaa catgagact gaaatgggg cgtggggcg 
      gatgatttt cgagtcgcc ggtgacacc gatcgtcgt 
      acccatctg gcatatggg tatggacga cgtcttgcc 
      caacgaacc cccgtacgt cctgcggct gggcaagaa 
      tttgacgtt taattatgg gaatcccat agacttgac 
      ggagatggt gcgcaagtt gaaagagac gaacgcgac 
      ggacgtgga ggcgaggaa gcaaataac cgacttttt 
      gcaagagca gttgctaaa gcttaagat tgaaaccga 
      agagttgca taagcggga ttttaaagt gaatttatg 
      atgttttag ggattagtt aggttgcga tttatttta 
      tttattgag tattgagta aagaacagc aatatttgg 
      atgctagtc acgtgtttt gcctggact ttacagtat 
      aaggcgagg tattaattt tttgcatta ttttgtgtc 
      gattctcat ttatttaaa gatcaaatc tttacatca 
      cgaatattt aatgccaaa atggtcaca gagggcgca 
      ttatttgat tgtgatatt tcttgcggg ggattctta 
      aagtttgta ggattcaac taacactag atttaggtt 
      ttttgcaat tggggactt gcataaaat gaatcgtag 
      atgatttga aattttgtt cccaaatgg cctatagtc 
      tctctagtt acagttaag ttgcatgtt ttataacat 
      atgccctga gacaggtca gcatttgca tgtaattcc 
      gttattata gcgcttgcg ccattttaa cacgtaatt 
      atgtcctta tacggcttg ggtgtctcc aggtgtttt 
      ttgcactgt atacaagtt cccaattac gaaatgtaa 
      cataaaaac gtagcttgt agcgtgtag gaacttgta 
      aaaaatgtc tcccctgtt ttaaagtag gtttagtta 
      tttcccatt tttataata aatctgcga cggatcaaa 
      tgatttttg tatgtaatt agttacgga aataaaagc 
      gattgacgc gatttttta gcattattg tgattgtct 
      gttgttttt cgattaggt ttgttaatt tgttgcggt 
      tattttgtt gtttaggtg aggttttag gtgttattt 
      ttatgatgc acttccatt ccgaaatcg ccgttttcc 
      tgaataaca gtcagtctg tttgctcct tgttagata 
      attttagaa actctgtac ctaaatatt ttattgttt 
      agattgttg tgcttctat tttttaaat aaattcgat 
      ttaatcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY