Status
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Gene information
General information
TC033699
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009026
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8497575..8505254
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033699
    iBeetle screen iB_02855

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    egg chamber sometimes potentiallyfused - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): more than 80%

    bristle partially remaining - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    Adult morphology
    flagellomeres slightly number decreased - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033699 RA
      MASHVLSVC VFCLICATH SPCGSHAAV AHENPQINL 
      QHDPILLKR EKFKEKEPW ENSVLTISD DKHVINKRH 
      SDDNDIKNK DFYLKKLFD KYGDGKTIT LEGFRNLLE 
      HVFEIQTIT HQEASRGDT NDTCVVNNA LISLKNSSN 
      SSETFNSST IEAACPALL YSMISGSCE PETKQNTAQ 
      PMTITVTTW LYSMCAVFV ISTCGVLSL LIIPVMQKK 
      FYKPLLQFL VALAVGTLA GDALLHLLP HAMSGHHHD 
      HGESHENMW KGFVAMLGL ILFFVMERF IPLVHRWRK 
      GKPSKGHSH VKVLNSKLD INKTGDTQC MDRYNAYPR 
      CYKDIVDDP NTTFMEAGE TSNVLEELL NSRKKKVTK 
      QQAAKGDSS ETAISKDCE NGETNQNLT ECPNDLNTS 
      NNCTEINKM LPHIEMAPH EEPEADYTV IITEHGAVH 
      HGHSHLHGH VHAAPQNFS SVAWMVIMG DGLHNFTDG 
      MAIGAAFSG SAAGGFSTA VAVFCHELP HEVGDFAML 
      LKAGMSIKQ ALFYNIISS ILCVLGNLL GVYLGNTEV 
      ASSWVFAVA AGTFIYIAL VDMIPELSS AHEDEGNLI 
      QCILHLSGL LLGFGIMTL IALFEHDLK NMFRE
    CDS sequence
    • >TC033699 RA
      atggcatct cacgtctta agcgtctgt gtcttttgc 
      ctcatctgc gccacccat tccccctgt ggttctcac 
      gcagctgtg gcccacgaa aacccccaa atcaacctc 
      cagcacgac cccattctc ctcaaacgt gaaaaattc 
      aaagagaaa gaaccctgg gagaacagt gttttaacc 
      ataagtgac gataaacac gttattaat aaaagacac 
      agtgatgac aatgatatt aaaaataaa gacttttat 
      ttaaaaaaa cttttcgat aaatacggt gacggcaaa 
      acaataaca ttggaaggt ttccgaaat ttgttggaa 
      cacgttttc gaaatacag acaattacg catcaagaa 
      gcttccagg ggtgacacc aacgacacg tgtgtcgtc 
      aataacgca ctaatctca ttaaaaaat tcatcaaat 
      tcgagcgaa acgttcaat tcgtcgaca atagaagca 
      gcatgcccg gccctcctc tactcgatg atttccgga 
      agctgcgaa ccggaaacg aaacaaaac acggcccag 
      ccaatgaca atcacggta acgacgtgg ttgtactca 
      atgtgtgcc gttttcgtg atcagcact tgtggggtt 
      ctttccctt ttgataatt cccgtgatg cagaaaaaa 
      ttctacaaa ccactttta caatttctg gtagcttta 
      gccgtcggc actcttgcc ggagacgcc cttctgcac 
      cttttgccc cacgctatg agcggccac caccatgac 
      cacggcgaa agccacgaa aatatgtgg aaaggcttc 
      gttgcgatg ctcggcctt atactattt tttgtgatg 
      gaaaggttc attcccttg gttcatagg tggcgcaag 
      ggcaagccg agcaaaggg cattcccat gtcaaagtc 
      ttaaatagt aaattagat ataaataaa acgggcgat 
      acgcaatgt atggatagg tataatgcg tatccgagg 
      tgctacaag gacattgtt gatgatccg aatacgact 
      tttatggaa gctggggag acatcaaat gtgctcgaa 
      gagctgctt aatagtcgc aaaaagaag gttactaaa 
      cagcaagcg gccaaaggc gactcgtcc gaaactgcg 
      atatccaaa gactgcgag aacggtgaa accaaccag 
      aacctaacc gagtgtccc aacgacctc aacacctcg 
      aacaactgc accgaaatc aacaagatg ctcccacac 
      atcgaaatg gctcctcac gaggaaccc gaggccgat 
      tacacggtt attataacc gagcacggg gctgtccac 
      catggccac tcccatctc catgggcac gtccatgcc 
      gccccccag aacttctcg tcggttgca tggatggtg 
      attatgggt gatggtctc cacaacttc actgatggt 
      atggcgata ggggctgcg ttttcgggg agtgccgcc 
      ggggggttt tccaccgcc gtggctgtc ttctgccac 
      gagttgccc catgaggtg ggcgacttc gccatgctg 
      ctcaaggcc ggcatgagt atcaaacag gcgctgttt 
      tataacatt atttcaagc atcttgtgc gtgttgggg 
      aatttgctg ggagtttat ttagggaat acggaagtt 
      gccagttct tgggtgttc gcagtggcg gctggaacg 
      tttatttat attgcgctg gtggatatg ataccagag 
      ttgagctcg gcacatgaa gatgaaggg aatttgatt 
      cagtgcata ttgcatttg agtgggctg ttattaggg 
      tttgggatt atgacgctg attgcgttg tttgaacat 
      gatcttaag aatatgttt agggaataa
    mRNA sequence
    • >TC033699 RA
      aggctggct tccatgttc atggttgcg cttgccgtg 
      cctggtgtg tttgccttt ataattaac acaaatcaa 
      agaccagtt tatcagctt taattagaa ttgtgtgtg 
      atgtgaggg acagttttt tcgcttagt ttgccgaat 
      tcaagtgat gtgtggtaa aattagaac cgatctgtg 
      aggttattg gcgtctgga atcacgatg gcatctcac 
      gtcttaagc gtctgtgtc ttttgcctc atctgcgcc 
      acccattcc ccctgtggt tctcacgca gctgtggcc 
      cacgaaaac ccccaaatc aacctccag cacgacccc 
      attctcctc aaacgtgaa aaattcaaa gagaaagaa 
      ccctgggag aacagtgtt ttaaccata agtgacgat 
      aaacacgtt attaataaa agacacagt gatgacaat 
      gatattaaa aataaagac ttttattta aaaaaactt 
      ttcgataaa tacggtgac ggcaaaaca ataacattg 
      gaaggtttc cgaaatttg ttggaacac gttttcgaa 
      atacagaca attacgcat caagaagct tccaggggt 
      gacaccaac gacacgtgt gtcgtcaat aacgcacta 
      atctcatta aaaaattca tcaaattcg agcgaaacg 
      ttcaattcg tcgacaata gaagcagca tgcccggcc 
      ctcctctac tcgatgatt tccggaagc tgcgaaccg 
      gaaacgaaa caaaacacg gcccagcca atgacaatc 
      acggtaacg acgtggttg tactcaatg tgtgccgtt 
      ttcgtgatc agcacttgt ggggttctt tcccttttg 
      ataattccc gtgatgcag aaaaaattc tacaaacca 
      cttttacaa tttctggta gctttagcc gtcggcact 
      cttgccgga gacgccctt ctgcacctt ttgccccac 
      gctatgagc ggccaccac catgaccac ggcgaaagc 
      cacgaaaat atgtggaaa ggcttcgtt gcgatgctc 
      ggccttata ctatttttt gtgatggaa aggttcatt 
      cccttggtt cataggtgg cgcaagggc aagccgagc 
      aaagggcat tcccatgtc aaagtctta aatagtaaa 
      ttagatata aataaaacg ggcgatacg caatgtatg 
      gataggtat aatgcgtat ccgaggtgc tacaaggac 
      attgttgat gatccgaat acgactttt atggaagct 
      ggggagaca tcaaatgtg ctcgaagag ctgcttaat 
      agtcgcaaa aagaaggtt actaaacag caagcggcc 
      aaaggcgac tcgtccgaa actgcgata tccaaagac 
      tgcgagaac ggtgaaacc aaccagaac ctaaccgag 
      tgtcccaac gacctcaac acctcgaac aactgcacc 
      gaaatcaac aagatgctc ccacacatc gaaatggct 
      cctcacgag gaacccgag gccgattac acggttatt 
      ataaccgag cacggggct gtccaccat ggccactcc 
      catctccat gggcacgtc catgccgcc ccccagaac 
      ttctcgtcg gttgcatgg atggtgatt atgggtgat 
      ggtctccac aacttcact gatggtatg gcgataggg 
      gctgcgttt tcggggagt gccgccggg gggttttcc 
      accgccgtg gctgtcttc tgccacgag ttgccccat 
      gaggtgggc gacttcgcc atgctgctc aaggccggc 
      atgagtatc aaacaggcg ctgttttat aacattatt 
      tcaagcatc ttgtgcgtg ttggggaat ttgctggga 
      gtttattta gggaatacg gaagttgcc agttcttgg 
      gtgttcgca gtggcggct ggaacgttt atttatatt 
      gcgctggtg gatatgata ccagagttg agctcggca 
      catgaagat gaagggaat ttgattcag tgcatattg 
      catttgagt gggctgtta ttagggttt gggattatg 
      acgctgatt gcgttgttt gaacatgat cttaagaat 
      atgtttagg gaataatga ctttataat tcgtttcta 
      ttggctgtt ttttatttt atctggttt ttgatactg 
      cgtgtttac tgcaaagac ttgtttttt ttacaatca 
      tcccccttc ttccttgtg ctgctactt aggtattag 
      tattgatta agacttacc tatattatg taaacccac 
      atgtaatga ccagtattg gtacgcaaa acaataaaa 
      tttatttaa atat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY