| General information | |||
| TC033685
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 7398542..7414125 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033685 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02852 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
low validity due to technical reasons - should be repeated: animals lost,
Pupal morphology
low validity due to technical reasons - should be repeated: animals lost,
Adult morphology
low validity due to technical reasons - should be repeated: animals lost,
- >TC033685 RA
MSYCMSPQD FDYPCSNRN KCKNSRKYR NDIHERPVR DTPGIITEP TISHFPPPE PPGPPSDWE LQYCKWSGW TWVKKSDKE VSRPQAEPN QFSDSPAEV SRAHHHPQD EKKKKKGGL FSCFKKSPP ESRASQNRT AVSNGPPPT PKPVQTEPQ PTNNDFNDS VSDGFDDCC CCGMDYECC CNVCPCMTQ VCCCCPREH EQGKRRESK TIETQTVKE MKEKATKMS EMHIPKPRP CCGRRRKCK CRCRRPVCC PCKQKTCCP CVQPVCCPC KSNQPNGSS SKRKNDDSS TINSDNIVN FCLANYLGS KKPNDFKSN PLVKSVDLL LKNNINIMN LFNDKKELK HQAKPIVSN QTISCLSSD NLIKRTDQE HNKLLTAVC DAILSSHKF DNDKFSSKP QFTESRINF KNSDSKESF NIFSSKEIL GRILSDLNF TMSKGLDGS DIPKETGVR NNGAPKIES FCYPRRSLT KLIRNKSRI LAAPRFPKK IEALRRLDS LKSGRDELK IKRGFGGHY RKVNGLKMD DSKKGRFDE SKLNFTDIF KNSEAVNKF KSDQKSPQM CSTSKQDTS KLESIEALL RTLDLTDDS TSVNFKPHE KTDIERNRL ETLLQDHQL QLLDSTSPP FTDFDLLLT NDEGRQEVQ DNNKPKENC TLCESCNAF LNRTVGSDN PRAIYPSIP AEHNVFYFF VIVMLATII RIVLIRYDI PTPYGAFMI MVGVIWGII CNHNLFVLL ASDAMNTNP VDLVCVYLP ALVFSTSFS LDVPIFLKT IPQLLVIGI FVTFLTGTL FGVLMRLVI EPSWSIKVG LTFGIFIVP INPASIVYL LKQQTTATR HISALLEGE AIFNMLLAE MTHDLIINF AKGFVVHWY QFVMVLIRF ILFGIAFGY FVGLLGRYM MRTLYRYPL TIFMISFGF PFFTFFLSD IYISGCGSI SVMILGTMM AMERSVLAK ETNQFLTDM WETIGYSLD AVMCVTITL LATVEVATI VTTSAYIRI IVTYLTYYS IRFICFLLF APIISRLGY GMDLKCMIV CVWSGLKSP FSLALSLIF VEDAISAEK MKRIFFQCV GIYFLSVLI NGTFTRKIL EFLGLREIS MARKINMTN CMKHIFTKR NKTVAILKM DRFLADVNW PVVLEATDM KHPYRMGME DTEEDNFFL LGYRFTVCP DCKSEIPKE PTTRELKDM AKEAKMRVL KSKKMCYAR QYENGMMTK EGIKILSQA VELAMDTEA AVIQLEGLE KRFKEENCI YRCIRKKVK HLNRSQADS IRPPRKYFR RLCYRICIN NTFEHFMLV IITIHCVFI TVSTIYHPP IDHLDTIDK KLDVAISVL DLIFSIIYI VEVILKIMA FSWIHFWEH GVKTYFKSV WRNIDFLVV VTCILQGYY DLKCFYAQI PESKYGVFY RVLTCLRLL RVVRLFTVL KIFYPKFVT YLDRSVDSQ LAFTYELGK SYAIGETEI LDMLPYMID NTAIREEIK QKIERDKVI ITKLLGMVQ KEKPWIAIT VKTKQAIRT ILNSMKEAA NQLRTAGWV DDYEQEKLF QVMGELWSK VNSIKSVQP SAPKVIFKE VAWMAGDQA VIDFLFENV TVKKFEPGD VVFEEGTVA DGIYIVVTG LFMIIYKPE SNVLTSLHE YGQLPIADY LSATQYEER VVDYIVSGN CIGELSTLT GRAYNCTVS ADAHCQVYV LSKGVIKRA MELNPDPVI GLECRLWKE VSIRMAVPL LMSVPAYQG FSQDQIKYA LERAFVPNL SNYKIFAVT DMIEDIILI EGCAADFNT RESFVAPCC IPRTVQKLI LPKSSLINI PVLLETKLL IIPEKDVDE YDVMILAED MCELVDTGA SMKCLQHAT QEKTRKKKV KMMAARKKG TAVSKTLSK LQPDSNDSV GMLFFKRTD SETNSINME EEEENKRNA EQTKHVILL VSIVRHVFV AYEIPIPHG VFIFVLGIC WGIGMKFSY WMYNMGCAF YFVVPLFKD VYLPVTVAS VNYFISSAL MCGLVSQMV IDPKNWKYT DGIFLGFVN SAIYPLDML RYLQENTIQ NKYVTSMLQ GETLFSTVL PWYIYQLTW LHMFGLVVY WYQFVMGFV RLIIGGLFL GYLLGSICV FLMKFSYND PSNLMVLTF AACFTNYYI GEYVLANAG ALTLVISSV MIAHERQSL TKEVEESVV NLWKMICSL MTNVIIFLT ASVLPDIVG TTFNYKDYI FVLVTYLMW NITRFISFF LFSPILSRI GYGMTFTNM IIYVWAGMK NPVCFSYSA IASKITQSH AILLHTVGV YLLMLLING SFTMCMLRV LGLAEISTS RRLNMNNCM KYLYEARSR TVAILKMDR FLSDANWPL VLSTTTLKH PYKKSKSAK NEDEDENDE AFTLGYKYT FCPDCEKDI PNEPTPKEM KEMLKEAKL RILKLKKTI YARQFESGM ISKEGLRIL HQAVEIAMD SEELTIELD GLYKLFTKE AVLKSKNLN YKVPRVKWK LWCYHLIKK TVFKSLIYI VIALNIGLL ITTFTLKPN TLLMNYTKL GLTILFFNF YLLVFATKL CAYYWSHKI TLALSTYFG FHENVVEHV ILCFSLTTL LCYAFKPLY HFTVVCTYL EALLLFSLF CRLLELLKF LRVIKAFKA LPDTIIKVL DSKVDTHRA MAFELAKNY VIAEEEILE IIHQIIDNV KIRAIIKDK IQEDRLAIV KRIGLFVKH GGWLLPTIK TTTALRAVL NSMKDDLHE LKISGWVDE VEYSKLFKS LGERTTYVN SIKSIPPTL PRKIFEEVP YIGDDSELI NFLYSNIAT KKFDPGDVI ITENDIAEG IYIVVTGMF LVSYTPNEN VTANLKIAG LLPGVDAIS SLHYEEPLV EYIIPGNTM GELSTISER PYHGIVTAE THSQVYVLN QDVVKKALQ MDTDPVTGL TCRIWKYIS FRKAITIML NVPSYRSYT LENIKYILE RSFVPDLSN FKIFAVNEM MSDIILIEG TVVDFNTRD VYTAPCYIP RAVQKLVMP KSSLINVEI AVETKLLVI PAKDYDGYD IMLTEEDTC ELVSDNASK CLRHLIKHK SRIGARASK YQSFMDSSS RVLKRRLQS VASQKISFA STQEYSVSF ESPHV
- >TC033685 RA
atgtcctac tgcatgtcc cctcaagac ttcgactac ccatgttcg aaccgaaac aaatgcaaa aattctcga aaatataga aacgacatt cacgaacgg cccgtaaga gacacccca ggcatcata accgaaccc accattagc cactttcct ccacccgag ccccccggc ccgcccagc gactgggaa ctccagtac tgcaaatgg tcggggtgg acttgggtg aaaaaaagc gacaaggag gtgtctcga cctcaagcc gaaccgaac caattctca gattcacct gcagaagtt tcccgcgcc catcatcat ccccaagat gagaagaag aagaaaaag gggggttta tttagctgt tttaagaaa agtccaccg gagagcagg gcttcgcag aatagaact gcggtgtca aacggtcca cccccaacc ccaaagccc gttcaaacc gaaccacaa ccaaccaat aacgacttt aatgactcg gtttcggac ggtttcgat gattgttgc tgttgtggg atggactac gagtgctgt tgtaacgtt tgtccttgt atgactcaa gtttgttgc tgttgtccg cgtgaacat gaacaagga aaacgacgg gaaagcaag accatagaa acacaaacc gtaaaagaa atgaaggaa aaagcgact aaaatgagc gaaatgcac atacccaag ccacgtcct tgttgtggg cgaaggcgt aaatgcaaa tgtaggtgt agaagacca gtctgttgt ccttgtaaa caaaaaact tgttgtcct tgtgtccag ccggtttgt tgtccttgt aaaagcaac caaccaaat gggagttcc tccaagagg aaaaatgat gactcctct acaataaat agtgacaat atagtaaat ttctgtttg gctaattat ttagggagt aagaaaccc aacgatttc aaatcgaat cccttagta aaatcagtc gatctcttg ttgaaaaat aacataaat atcatgaat ctattcaac gacaaaaaa gagctgaaa caccaggcc aagcccatc gtttcaaac caaacaata agttgccta tcgtccgat aatctcata aaaaggacc gaccaggag cataataaa ttactaacc gcagtttgt gatgcaatt ctgtccagt cacaaattc gataatgac aagttcagt tcaaagccc cagtttaca gaatcccgc attaatttc aaaaattca gattcgaaa gaatcgttt aatattttt tcgagtaaa gaaatcctt ggacggatt ttgagcgat ttaaatttt acaatgagt aagggcctg gacggctca gatatcccc aaagaaacg ggggtacgt aataatggg gctccaaaa atcgagagt ttttgttac ccaagacga tccttgacg aagttgata agaaataag agtcgcatt ttggcagcc ccccgtttt ccgaaaaaa atagaagca ttgcgaagg ctagattcg ctgaagtct gggcgtgat gaattgaaa ataaaacga gggtttggg ggccactat aggaaagtg aatgggctg aaaatggat gattctaag aaggggagg ttcgatgag agtaaatta aacttcacc gatattttc aaaaatagc gaagcagtg aataaattc aagtcggat cagaaaagt ccccaaatg tgttcaacg tctaaacaa gacacctcc aaattggaa tctatagaa gcgctcttg aggacactc gatttaacg gatgactca actagtgtt aattttaag cctcatgaa aaaacggat attgaaaga aatcgactt gagactctt cttcaggat caccaacta cagttgctg gactcgact tcacctcca ttcaccgat tttgatttg cttttgacc aacgatgag ggcaggcaa gaagtgcag gataataat aaaccaaag gagaactgc actctttgc gaatcatgt aacgctttc ctcaatagg acagttggt tcggacaat ccccgtgcg atataccct tcaattcca gccgaacat aatgtattt tacttcttc gtgatcgtc atgttggca accataatc cgaatcgtc ctgatacgc tacgacatc cctacccct tacggcgct ttcatgatt atggtcggg gttatctgg ggaatcatc tgcaaccac aatctcttc gtccttctt gcttccgac gccatgaat accaatcct gtcgatctt gtctgcgtt tacttaccc gctttggtg ttctccacc agtttcagc ctcgacgtc cctattttc ctcaagact attccgcaa ctcctagtg attggaatt ttcgtaact tttctaacc ggaaccctt tttggagtc ctaatgagg ttggtgatt gagccgagt tggtccatt aaagttggt ctaactttc ggtattttt atcgttccg atcaatccc gcaagcatt gtgtatctt ttgaagcag caaacaaca gcgaccaga cacatcagc gctctcttg gaaggcgag gccatcttc aatatgctt cttgcggaa atgacccac gatcttatt attaacttt gcaaaaggg tttgtcgtt cattggtat cagtttgta atggtgtta atacgcttt attttgttt ggtatcgca tttgggtat ttcgtgggc cttcttggt cgctacatg atgcgcact ttgtaccgc taccctctt actatcttc atgatttct ttcggtttt cccttcttc acgttcttt ttatcagat atttacata tctggttgt gggtctatt tccgttatg attctgggt accatgatg gcgatggaa cgctcagtt cttgcgaaa gagacaaat cagtttttg actgacatg tgggaaacc attggatat tctcttgat gcagtcatg tgcgtgact attacactg ctggccact gtcgaagtg gccacaatt gtgactaca tcggcttat attcgtata attgtgact tatctgacc tattactcg atcagattc atctgtttc ctcttgttt gcgcccatc atcagccga ttgggctat ggcatggac ttgaaatgc atgatagtg tgtgtgtgg agcggcctc aagagtccc tttagtcta gctctttcc ttaatcttc gtagaggat gcaatatcc gccgaaaaa atgaaacga atctttttc caatgtgtt ggcatttat tttctgtca gttttaatc aatgggact tttacgcga aaaatcttg gagttctta ggcctgcga gaaatatcc atggcgcga aaaatcaac atgacaaac tgcatgaaa cacattttc accaaaagg aacaaaacg gtggccatc ctcaagatg gacaggttt ttagccgac gtcaactgg ccggtcgtc cttgaagcc actgacatg aaacatcca taccgtatg ggaatggaa gacaccgaa gaagacaat ttttttttg ttagggtac cggttcacc gtttgcccc gactgtaaa agcgaaatt cccaaagaa ccgaccact agagaacta aaagacatg gctaaggag gccaaaatg cgagtgctg aaaagcaaa aaaatgtgc tatgcgcgt cagtacgag aacggcatg atgaccaaa gaagggatc aagattctt agtcaagcc gtcgagctt gcgatggac actgaggct gcagtgatc caactcgag ggcctcgag aaacgattc aaggaagag aattgtatc taccgatgc attcgaaag aaggtcaaa catttgaac cgatctcaa gccgactca atcagacct cctcgtaaa tatttcaga cggctttgc taccggatt tgcatcaat aatacgttc gaacatttt atgttggtc atcataact atacattgc gttttcata accgtgagc acgatatat caccccccg atagaccac ttagacacc atagataaa aaacttgac gttgcgatc tcagtcctt gatctcatc tttagtatc atttacatc gtggaggtg attttgaaa ataatggca ttttcttgg atccacttt tgggaacac ggagttaag acctatttt aagtctgtt tggcgtaat atcgatttc ttggtagtg gtgacatgt atattgcaa ggttactac gacttgaag tgtttttat gcacaaatc cctgagagc aagtatggt gtcttttat cgcgtttta acatgtttg aggttactg cgagtagtg cgactcttc acagttttg aaaatcttc tacccaaaa tttgtaaca tatctagac cgctctgtg gatagccaa ttagcgttt acgtacgag ttgggaaag agttatgca attggggaa acagaaatt ttggatatg ttgccctac atgattgac aataccgca atccgagaa gaaatcaag cagaaaatt gaaagagac aaggttatc ataacaaag ttgctggga atggttcag aaggagaaa ccttggatt gcaataact gtgaagacg aaacaagca attcggacg attttgaac tcaatgaaa gaagcagca aatcagttg aggactgct ggttgggtc gacgactat gagcaagaa aaactgttc caagtcatg ggcgaattg tggtccaaa gtgaattca atcaagtcg gtgcaacca agcgccccc aaagtcatt ttcaaggaa gttgcgtgg atggcagga gatcaggcg gttattgat tttctgttc gaaaatgtc actgtaaaa aagttcgaa cctggagat gtggttttt gaggaaggg actgttgct gatggaatt tacatcgtc gtgactggg ctctttatg atcatttat aaaccggag tcaaacgta ttgactagt ttgcacgaa tacggccaa ttgccgatt gctgattat ttatcggcg acacaatac gaagaacgt gttgtagac tatatcgtg tcgggtaat tgtataggc gagcttagt actttaact ggaagagct tataactgt actgtgtcg gcagacgca cattgccaa gtctatgtt ttgagtaaa ggtgtcata aaacgcgca atggaacta aatccagac cctgtcatt ggactggaa tgtcggctt tggaaagaa gtcagtata aggatggcg gtccctctc ctcatgtca gttccagct taccaaggc ttctctcaa gaccaaatt aaatacgcc cttgaacgt gccttcgtc ccaaatttg tccaattac aagattttc gccgtcacc gacatgatt gaagacatt attttaatc gaaggctgt gctgctgat ttcaatact cgagaaagt tttgttgcc ccttgttgt attcccaga acagtccag aaactcata cttccaaag agtagttta attaacatt ccagttctc ttagaaacg aaactactt ataattccc gagaaagat gtagatgaa tatgatgtc atgattttg gctgaagat atgtgcgaa ttggtggat actggagct agtatgaag tgcctccaa catgcgaca caagagaag acgcggaag aaaaaggtt aaaatgatg gctgcgagg aaaaaaggg actgccgtt agtaaaact ttgagcaag ttgcagcct gattcgaat gattctgtt ggtatgttg ttcttcaag agaaccgac tcagaaaca aacagtatt aacatggaa gaggaggaa gagaataaa cgaaatgct gagcagacg aagcatgtc atattgctg gtatcaatc gttcgtcat gtttttgtt gcgtacgaa atacccatc ccccacgga gtcttcatc tttgtccta ggtatttgt tggggtatc ggaatgaaa ttttcttat tggatgtat aacatggga tgcgcattt tattttgtc gtaccactt tttaaggat gtgtacctc ccggtcact gtcgcaagt gttaattat ttcatttcc agtgctctt atgtgtggc ttggtctca cagatggtc atagaccca aaaaactgg aaatacacc gatgggatc ttccttggt tttgtgaac agtgcgatt taccctttg gacatgttg cgctatctt caagaaaac accatccag aacaagtac gtcacatcg atgcttcaa ggcgagacg cttttttcg acggttctt ccgtggtat atttaccag ctgacgtgg ttgcacatg tttggtttg gtagtgtac tggtaccag tttgtcatg gggtttgtg cggttgata attggcggt cttttccta ggttacctc ttaggctcc atctgtgtc ttcttgatg aaattctca tacaacgac ccttcgaat ctgatggtt cttaccttt gccgcttgc tttaccaat tattacatc ggggagtac gttctggcc aatgcggga gcactcacc cttgtaata tcatctgta atgatcgcc cacgagcgc cagtctctt acaaaagaa gtagaagaa tcggttgtg aatctttgg aaaatgatt tgttcctta atgacgaat gtcataatt tttttaacg gccagcgtt ctccctgat atagtcgga accactttt aattacaaa gactacata ttcgtttta gtcacctat ttgatgtgg aacattact cgctttatc tcgttcttt ctgttttcg ccaattctg agtaggatc ggttacgga atgactttt acaaatatg atcatttat gtttgggct ggtatgaaa aatccagtt tgtttttcc tattccgct atagcatcc aaaataaca caaagccat gcaatttta ctccatacg gtcggcgtc tatctttta atgcttttg atcaatgga agtttcaca atgtgtatg cttcgagtc ctgggactt gcagaaatt tcaacatcc cgaagactg aatatgaac aattgtatg aaatatctg tatgaagcc agaagtcgc actgtcgcc atattgaag atggacagg ttcctgtct gatgccaac tggcccttg gtcctaagc actacaaca ctgaaacat ccatataag aagtccaaa tctgcaaaa aatgaagac gaagatgag aatgatgag gctttcacc cttggatac aagtatacc ttttgtcct gattgcgaa aaagacata ccaaatgaa cccactccc aaagaaatg aaagagatg ctcaaagaa gcaaaactt cgaatcctc aaattaaag aaaactatt tatgcgaga caatttgaa agcggaatg atatccaaa gaagggctc aggatcttg caccaagct gtcgaaatt gcaatggac agtgaagag ctcaccatc gaacttgat ggtctttac aaactgttc acaaaagag gctgtcctg aaatcgaaa aatctaaac tacaaagtg ccacgggtt aagtggaag ttgtggtgt taccactta atcaaaaag accgttttc aaatccctg atatatatt gtcatagca ctcaatatt ggattactt atcacaact ttcacactg aagccaaac acactcctg atgaattac actaaactt ggcttaact attttattt tttaatttc taccttctc gtattcgca acaaaactg tgtgcatat tattggtca cataagata acacttgca ttgtcgact tactttggg tttcatgaa aatgtggtt gagcatgta attttgtgt ttctcatta accacacta ttatgctac gctttcaaa cccttgtac cattttaca gtagtttgt acctatttg gaagcactt ctcctattt agtcttttt tgccgtttg ttggaattg ttgaaattt ctgcgcgtt attaaagcc ttcaaagcc ctgcctgat acgataatt aaagttttg gatagtaaa gtcgatact cacagagca atggccttt gaactggcc aaaaactat gtcattgcc gaagaagag attttggaa attattcat caaataatc gacaatgtt aaaatacga gcaatcata aaagacaaa atccaagaa gatcgcctg gcgattgtt aaaagaata ggtctgttt gttaaacat ggagggtgg cttctgccc accatcaag acaacaacc gctttacgc gctgttcta aacagcatg aaagacgac ttacatgaa ctaaaaatc tcaggatgg gtggatgag gtcgaatac agcaaactt tttaaatct ctgggtgaa agaactacg tacgttaac agcataaaa agcatcccg ccaacgttg cccaggaag atatttgaa gaagtacca tacattggt gacgacagc gaattaatt aattttctg tacagtaac attgctact aaaaaattt gatcccggg gatgttatc atcactgaa aacgatatc gctgaaggc atctacatt gtcgtgaca ggaatgttc ttagtaagc tacacgcca aacgaaaat gttactgca aatttaaag atagcaggt ctgctaccg ggagttgac gcaatatcg tccctccac tacgaggag cctttagta gagtacata atcccaggg aacacgatg ggggagctg tccacaatt tcggaacga ccatatcat ggcatcgtc actgctgag acccacagt caagtctat gtcctcaac caggatgtt gtaaaaaaa gcccttcag atggacaca gatcccgtc acaggccta acttgtcga atttggaag tacatcagt tttcgtaaa gcaattacg attatgctg aacgtccca tcttaccgt tcttacact ttggaaaat attaaatat attctcgaa cggtcattc gtcccggac ttatcaaat tttaaaatt tttgccgtg aatgaaatg atgagcgac attatacta attgaaggt actgttgtc gattttaat actcgggat gtgtatact gcaccttgc tacatacct agggcggta caaaaactg gtaatgccc aaaagcagt cttattaat gttgagata gcagttgag actaaattg ctagttatt ccggctaag gactatgat gggtacgac ataatgcta acagaagaa gacacttgt gaattggtc agtgataat gcaagtaaa tgtcttcgc caccttata aaacataaa tctagaatc ggggcaagg gctagcaag tatcagtca tttatggat tctagttca agggttctt aaaagaagg ctacaaagt gttgcttcc caaaaaatt tcatttgca agcacacaa gaatatagt gtatcgttt gaatcacca catgtctga
- >TC033685 RA
atgtaagaa aacagacag taatacttg gaccgaaaa aaatgctgc atgggatga aatcactga agagtttaa tgctcagtc gctcagtca aacctttcg atcaaaaga atcgttaaa aaaatgtta tgataacag aaaaaaaga aatgcgaag actagcggc gcaaaatcg ccaggaatt tataactgg aggaggtac tggccaaca actcaacga tccttgttt ggaaattat aatggatat cacaaatgg aaaatctta tattccgaa tgtcatcca aacgtcatt tcacaacat tttgtttat ttttcgatt gtgttacca aaaatttcc gccggttat gtgtcaaag aacctataa tcccccttt ataatgtcc tactgcatg tcccctcaa gacttcgac tacccatgt tcgaaccga aacaaatgc aaaaattct cgaaaatat agaaacgac attcacgaa cggcccgta agagacacc ccaggcatc ataaccgaa cccaccatt agccacttt cctccaccc gagcccccc ggcccgccc agcgactgg gaactccag tactgcaaa tggtcgggg tggacttgg gtgaaaaaa agcgacaag gaggtgtct cgacctcaa gccgaaccg aaccaattc tcagattca cctgcagaa gtttcccgc gcccatcat catccccaa gatgagaag aagaagaaa aaggggggt ttatttagc tgttttaag aaaagtcca ccggagagc agggcttcg cagaataga actgcggtg tcaaacggt ccaccccca accccaaag cccgttcaa accgaacca caaccaacc aataacgac tttaatgac tcggtttcg gacggtttc gatgattgt tgctgttgt gggatggac tacgagtgc tgttgtaac gtttgtcct tgtatgact caagtttgt tgctgttgt ccgcgtgaa catgaacaa ggaaaacga cgggaaagc aagaccata gaaacacaa accgtaaaa gaaatgaag gaaaaagcg actaaaatg agcgaaatg cacataccc aagccacgt ccttgttgt gggcgaagg cgtaaatgc aaatgtagg tgtagaaga ccagtctgt tgtccttgt aaacaaaaa acttgttgt ccttgtgtc cagccggtt tgttgtcct tgtaaaagc aaccaacca aatgggagt tcctccaag aggaaaaat gatgactcc tctacaata aatagtgac aatatagta aatttctgt ttggctaat tatttaggg agtaagaaa cccaacgat ttcaaatcg aatccctta gtaaaatca gtcgatctc ttgttgaaa aataacata aatatcatg aatctattc aacgacaaa aaagagctg aaacaccag gccaagccc atcgtttca aaccaaaca ataagttgc ctatcgtcc gataatctc ataaaaagg accgaccag gagcataat aaattacta accgcagtt tgtgatgca attctgtcc agtcacaaa ttcgataat gacaagttc agttcaaag ccccagttt acagaatcc cgcattaat ttcaaaaat tcagattcg aaagaatcg tttaatatt ttttcgagt aaagaaatc cttggacgg attttgagc gatttaaat tttacaatg agtaagggc ctggacggc tcagatatc cccaaagaa acgggggta cgtaataat ggggctcca aaaatcgag agtttttgt tacccaaga cgatccttg acgaagttg ataagaaat aagagtcgc attttggca gccccccgt tttccgaaa aaaatagaa gcattgcga aggctagat tcgctgaag tctgggcgt gatgaattg aaaataaaa cgagggttt gggggccac tataggaaa gtgaatggg ctgaaaatg gatgattct aagaagggg aggttcgat gagagtaaa ttaaacttc accgatatt ttcaaaaat agcgaagca gtgaataaa ttcaagtcg gatcagaaa agtccccaa atgtgttca acgtctaaa caagacacc tccaaattg gaatctata gaagcgctc ttgaggaca ctcgattta acggatgac tcaactagt gttaatttt aagcctcat gaaaaaacg gatattgaa agaaatcga cttgagact cttcttcag gatcaccaa ctacagttg ctggactcg acttcacct ccattcacc gattttgat ttgcttttg accaacgat gagggcagg caagaagtg caggataat aataaacca aaggagaac tgcactctt tgcgaatca tgtaacgct ttcctcaat aggacagtt ggttcggac aatccccgt gcgatatac ccttcaatt ccagccgaa cataatgta ttttacttc ttcgtgatc gtcatgttg gcaaccata atccgaatc gtcctgata cgctacgac atccctacc ccttacggc gctttcatg attatggtc ggggttatc tggggaatc atctgcaac cacaatctc ttcgtcctt cttgcttcc gacgccatg aataccaat cctgtcgat cttgtctgc gtttactta cccgctttg gtgttctcc accagtttc agcctcgac gtccctatt ttcctcaag actattccg caactccta gtgattgga attttcgta acttttcta accggaacc ctttttgga gtcctaatg aggttggtg attgagccg agttggtcc attaaagtt ggtctaact ttcggtatt tttatcgtt ccgatcaat cccgcaagc attgtgtat cttttgaag cagcaaaca acagcgacc agacacatc agcgctctc ttggaaggc gaggccatc ttcaatatg cttcttgcg gaaatgacc cacgatctt attattaac tttgcaaaa gggtttgtc gttcattgg tatcagttt gtaatggtg ttaatacgc tttattttg tttggtatc gcatttggg tatttcgtg ggccttctt ggtcgctac atgatgcgc actttgtac cgctaccct cttactatc ttcatgatt tctttcggt tttcccttc ttcacgttc tttttatca gatatttac atatctggt tgtgggtct atttccgtt atgattctg ggtaccatg atggcgatg gaacgctca gttcttgcg aaagagaca aatcagttt ttgactgac atgtgggaa accattgga tattctctt gatgcagtc atgtgcgtg actattaca ctgctggcc actgtcgaa gtggccaca attgtgact acatcggct tatattcgt ataattgtg acttatctg acctattac tcgatcaga ttcatctgt ttcctcttg tttgcgccc atcatcagc cgattgggc tatggcatg gacttgaaa tgcatgata gtgtgtgtg tggagcggc ctcaagagt ccctttagt ctagctctt tccttaatc ttcgtagag gatgcaata tccgccgaa aaaatgaaa cgaatcttt ttccaatgt gttggcatt tattttctg tcagtttta atcaatggg acttttacg cgaaaaatc ttggagttc ttaggcctg cgagaaata tccatggcg cgaaaaatc aacatgaca aactgcatg aaacacatt ttcaccaaa aggaacaaa acggtggcc atcctcaag atggacagg tttttagcc gacgtcaac tggccggtc gtccttgaa gccactgac atgaaacat ccataccgt atgggaatg gaagacacc gaagaagac aattttttt ttgttaggg taccggttc accgtttgc cccgactgt aaaagcgaa attcccaaa gaaccgacc actagagaa ctaaaagac atggctaag gaggccaaa atgcgagtg ctgaaaagc aaaaaaatg tgctatgcg cgtcagtac gagaacggc atgatgacc aaagaaggg atcaagatt cttagtcaa gccgtcgag cttgcgatg gacactgag gctgcagtg atccaactc gagggcctc gagaaacga ttcaaggaa gagaattgt atctaccga tgcattcga aagaaggtc aaacatttg aaccgatct caagccgac tcaatcaga cctcctcgt aaatatttc agacggctt tgctaccgg atttgcatc aataatacg ttcgaacat tttatgttg gtcatcata actatacat tgcgttttc ataaccgtg agcacgata tatcacccc ccgatagac cacttagac accatagat aaaaaactt gacgttgcg atctcagtc cttgatctc atctttagt atcatttac atcgtggag gtgattttg aaaataatg gcattttct tggatccac ttttgggaa cacggagtt aagacctat tttaagtct gtttggcgt aatatcgat ttcttggta gtggtgaca tgtatattg caaggttac tacgacttg aagtgtttt tatgcacaa atccctgag agcaagtat ggtgtcttt tatcgcgtt ttaacatgt ttgaggtta ctgcgagta gtgcgactc ttcacagtt ttgaaaatc ttctaccca aaatttgta acatatcta gaccgctct gtggatagc caattagcg tttacgtac gagttggga aagagttat gcaattggg gaaacagaa attttggat atgttgccc tacatgatt gacaatacc gcaatccga gaagaaatc aagcagaaa attgaaaga gacaaggtt atcataaca aagttgctg ggaatggtt cagaaggag aaaccttgg attgcaata actgtgaag acgaaacaa gcaattcgg acgattttg aactcaatg aaagaagca gcaaatcag ttgaggact gctggttgg gtcgacgac tatgagcaa gaaaaactg ttccaagtc atgggcgaa ttgtggtcc aaagtgaat tcaatcaag tcggtgcaa ccaagcgcc cccaaagtc attttcaag gaagttgcg tggatggca ggagatcag gcggttatt gattttctg ttcgaaaat gtcactgta aaaaagttc gaacctgga gatgtggtt tttgaggaa gggactgtt gctgatgga atttacatc gtcgtgact gggctcttt atgatcatt tataaaccg gagtcaaac gtattgact agtttgcac gaatacggc caattgccg attgctgat tatttatcg gcgacacaa tacgaagaa cgtgttgta gactatatc gtgtcgggt aattgtata ggcgagctt agtacttta actggaaga gcttataac tgtactgtg tcggcagac gcacattgc caagtctat gttttgagt aaaggtgtc ataaaacgc gcaatggaa ctaaatcca gaccctgtc attggactg gaatgtcgg ctttggaaa gaagtcagt ataaggatg gcggtccct ctcctcatg tcagttcca gcttaccaa ggcttctct caagaccaa attaaatac gcccttgaa cgtgccttc gtcccaaat ttgtccaat tacaagatt ttcgccgtc accgacatg attgaagac attatttta atcgaaggc tgtgctgct gatttcaat actcgagaa agttttgtt gccccttgt tgtattccc agaacagtc cagaaactc atacttcca aagagtagt ttaattaac attccagtt ctcttagaa acgaaacta cttataatt cccgagaaa gatgtagat gaatatgat gtcatgatt ttggctgaa gatatgtgc gaattggtg gatactgga gctagtatg aagtgcctc caacatgcg acacaagag aagacgcgg aagaaaaag gttaaaatg atggctgcg aggaaaaaa gggactgcc gttagtaaa actttgagc aagttgcag cctgattcg aatgattct gttggtatg ttgttcttc aagagaacc gactcagaa acaaacagt attaacatg gaagaggag gaagagaat aaacgaaat gctgagcag acgaagcat gtcatattg ctggtatca atcgttcgt catgttttt gttgcgtac gaaataccc atcccccac ggagtcttc atctttgtc ctaggtatt tgttggggt atcggaatg aaattttct tattggatg tataacatg ggatgcgca ttttatttt gtcgtacca ctttttaag gatgtgtac ctcccggtc actgtcgca agtgttaat tatttcatt tccagtgct cttatgtgt ggcttggtc tcacagatg gtcatagac ccaaaaaac tggaaatac accgatggg atcttcctt ggttttgtg aacagtgcg atttaccct ttggacatg ttgcgctat cttcaagaa aacaccatc cagaacaag tacgtcaca tcgatgctt caaggcgag acgcttttt tcgacggtt cttccgtgg tatatttac cagctgacg tggttgcac atgtttggt ttggtagtg tactggtac cagtttgtc atggggttt gtgcggttg ataattggc ggtcttttc ctaggttac ctcttaggc tccatctgt gtcttcttg atgaaattc tcatacaac gacccttcg aatctgatg gttcttacc tttgccgct tgctttacc aattattac atcggggag tacgttctg gccaatgcg ggagcactc acccttgta atatcatct gtaatgatc gcccacgag cgccagtct cttacaaaa gaagtagaa gaatcggtt gtgaatctt tggaaaatg atttgttcc ttaatgacg aatgtcata attttttta acggccagc gttctccct gatatagtc ggaaccact tttaattac aaagactac atattcgtt ttagtcacc tatttgatg tggaacatt actcgcttt atctcgttc tttctgttt tcgccaatt ctgagtagg atcggttac ggaatgact tttacaaat atgatcatt tatgtttgg gctggtatg aaaaatcca gtttgtttt tcctattcc gctatagca tccaaaata acacaaagc catgcaatt ttactccat acggtcggc gtctatctt ttaatgctt ttgatcaat ggaagtttc acaatgtgt atgcttcga gtcctggga cttgcagaa atttcaaca tcccgaaga ctgaatatg aacaattgt atgaaatat ctgtatgaa gccagaagt cgcactgtc gccatattg aagatggac aggttcctg tctgatgcc aactggccc ttggtccta agcactaca acactgaaa catccatat aagaagtcc aaatctgca aaaaatgaa gacgaagat gagaatgat gaggctttc acccttgga tacaagtat accttttgt cctgattgc gaaaaagac ataccaaat gaacccact cccaaagaa atgaaagag atgctcaaa gaagcaaaa cttcgaatc ctcaaatta aagaaaact atttatgcg agacaattt gaaagcgga atgatatcc aaagaaggg ctcaggatc ttgcaccaa gctgtcgaa attgcaatg gacagtgaa gagctcacc atcgaactt gatggtctt tacaaactg ttcacaaaa gaggctgtc ctgaaatcg aaaaatcta aactacaaa gtgccacgg gttaagtgg aagttgtgg tgttaccac ttaatcaaa aagaccgtt ttcaaatcc ctgatatat attgtcata gcactcaat attggatta cttatcaca actttcaca ctgaagcca aacacactc ctgatgaat tacactaaa cttggctta actatttta ttttttaat ttctacctt ctcgtattc gcaacaaaa ctgtgtgca tattattgg tcacataag ataacactt gcattgtcg acttacttt gggtttcat gaaaatgtg gttgagcat gtaattttg tgtttctca ttaaccaca ctattatgc tacgctttc aaacccttg taccatttt acagtagtt tgtacctat ttggaagca cttctccta tttagtctt ttttgccgt ttgttggaa ttgttgaaa tttctgcgc gttattaaa gccttcaaa gccctgcct gatacgata attaaagtt ttggatagt aaagtcgat actcacaga gcaatggcc tttgaactg gccaaaaac tatgtcatt gccgaagaa gagattttg gaaattatt catcaaata atcgacaat gttaaaata cgagcaatc ataaaagac aaaatccaa gaagatcgc ctggcgatt gttaaaaga ataggtctg tttgttaaa catggaggg tggcttctg cccaccatc aagacaaca accgcttta cgcgctgtt ctaaacagc atgaaagac gacttacat gaactaaaa atctcagga tgggtggat gaggtcgaa tacagcaaa ctttttaaa tctctgggt gaaagaact acgtacgtt aacagcata aaaagcatc ccgccaacg ttgcccagg aagatattt gaagaagta ccatacatt ggtgacgac agcgaatta attaatttt ctgtacagt aacattgct actaaaaaa tttgatccc ggggatgtt atcatcact gaaaacgat atcgctgaa ggcatctac attgtcgtg acaggaatg ttcttagta agctacacg ccaaacgaa aatgttact gcaaattta aagatagca ggtctgcta ccgggagtt gacgcaata tcgtccctc cactacgag gagccttta gtagagtac ataatccca gggaacacg atgggggag ctgtccaca atttcggaa cgaccatat catggcatc gtcactgct gagacccac agtcaagtc tatgtcctc aaccaggat gttgtaaaa aaagccctt cagatggac acagatccc gtcacaggc ctaacttgt cgaatttgg aagtacatc agttttcgt aaagcaatt acgattatg ctgaacgtc ccatcttac cgttcttac actttggaa aatattaaa tatattctc gaacggtca ttcgtcccg gacttatca aattttaaa atttttgcc gtgaatgaa atgatgagc gacattata ctaattgaa ggtactgtt gtcgatttt aatactcgg gatgtgtat actgcacct tgctacata cctagggcg gtacaaaaa ctggtaatg cccaaaagc agtcttatt aatgttgag atagcagtt gagactaaa ttgctagtt attccggct aaggactat gatgggtac gacataatg ctaacagaa gaagacact tgtgaattg gtcagtgat aatgcaagt aaatgtctt cgccacctt ataaaacat aaatctaga atcggggca agggctagc aagtatcag tcatttatg gattctagt tcaagggtt cttaaaaga aggctacaa agtgttgct tcccaaaaa atttcattt gcaagcaca caagaatat agtgtatcg tttgaatca ccacatgtc tgatcgcat taaagtaaa taatgtgtt
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