| General information | |||
| TC033683
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid49: 722064..729990 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033683 | ||
| General information | ||||
| iB_02851 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC033683 RA
MAGYVEHWY QMISVLLRF LVVGTLEGV LFGLAGRFL VRKMSWNKL SMFSIFFVM PFLCFSFAF NFGSGSGTT GVAILGLIL GLERTTLSK EADKHITNF WEAVGFIMD VILCVRCAI ASMLYVIEV VSWNHFVTV LVTYLIYYV TRFFCFLLF LPLLSRLGY GMGFKNMII CVWCGIKSP FSVLLVASM ATIDKSNLY RHIWLSISF FVIGLYTLS NLINGSLTR SLLNFMGFR TISIARQAN MSNCIKHIF DKRERTIEI LKMDRFLAD SDWALIRET SVIKHPYHV NVNSTAEEE EQYFLGYRY TYCPECKKD VEEQPTAKE LNEIRKEAQ MRVLKAKKV CYTRQFEKG MVSKPGMRL LSQGLEIEL DTGEIYEVN FDHLEKKFS LCIDRYADT KLAFAYDIC KSYVSTNSE VLEMLPYLI DNKQVRDEV KMKLDSDRV VITKMLGFI QKEKPWVAI TVKTKQAIR TILSNMSEA LNQLKVAGW IDNFEQDKL LMAMDVLYE RVNAIKMVQ PSAPKLIFK EVAWMAEDE RVINYLFEN VTLKKFDTG DIVFEEGDI ADCIYIVVT GLFVVNYTP NKICLENLQ AYGRLPIVD YLSSTKYDI PSVDYIVSG NCIGELSFL TERPYNCTI QAEAPSQVF VLKSEFLRR AMKMSPDPV IGLECRIWK EVSIRTAVP LLLATPAYQ LLSQEQVKY ALERAFVPN LSQYKIFAV TDMIEDVII VDGFVADYN TRETYLAPC CISRTVQKL ILPSSSYMN VSFDVETKL LIIPDQEVD EYDVMMMAE ETCEMNTRI PIPYQVFML ASASVWGVI MYYNEVPLL FSKAIDVPM VDVALLYVP AAIFNISFC MDVPIFFKS IPQILIIGL PVAGLTGIF NGILMRQLI NDKWPLAFG FFFGLLCIP TNPKFIGYL LKEQSLRTK HIGVLLEGE ALISFVLGD YLYLLTVSY MSKHVVHWY QIISVILRF VVMGILEGL LFGMMGRYL VRKMSWNKL SMFSIFFVM PFLCFSFAF NFGSGCGTT GVAILGLIM GLERTTLSR EAEKYLTNF WEGLGFIMD VLLCVRCAV DAMIYVVGK VEWSHYVLI LVVYLVYYV TRFSCYLLF LPLLSRLGY GMDFKNMMI CVWCGIKNP LSLLMVASM AVIDVSRSY DEIWRLCFF FFLGLYTLS NLINGSLTR NLLNFMGFR TISMSRQAN MSNCIKHIF NKRQRTIDI LKMDRFLAD NDWSLIRKI CTIEHPYHI DLTTEEEEQ LFLGYRYTY CPECKKDVE EQPTAKEIK EIMKEAQMR VLKAKKICY TRQFERGMV SKPGMRLLS QALEVELDT GEIYQVNLD NVEKQFRLN ICQRLFRKL LLRIQRLRT SCLRPKTYW RRVCYYLVI KTSYLDILM FVIVILNIV VIGIDWRYH STFTTDSTH LEPSHKLNY VITTIKLTF IFVYFSEFI IKVMAYSRR SICADGLKR YFKSLANTI DVMIIFILI VNVTFVTNE TVNLSHNHE RSEEEETVH LVFSWLQLL VIVKLAVVL RIFNPLLLN CLERKADAK MALAYDICK SYAMSNSEV LEMLPYLID NKQVREEVK MKLNCDRVL ITKMLGLIQ KEKPWVAIT VKTKQAIRT ILSNMSEAV NHLRVGGWI DNFEQEKLL LAMDVLYDR VNAIEMVQP SAPKVIFKE VAWMAEDED VINFLFENV TVKKFDTGD VVFDEGDVA DGIYIVVTG
- >TC033683 RA
atggcggga tacgtggaa cattggtac cagatgatt agtgtgtta ctccgtttt cttgttgtc ggtactttg gaaggagtc ttatttggg ttagccggt cgttttctc gtgcgaaaa atgtcatgg aacaagttg agcatgttt tcaatattt ttcgtgatg ccgtttttg tgtttttct ttcgcattt aactttggc tccggttct ggcacgacg ggggtagcg atcctcggt cttatattg ggtctcgaa cgaacaact ttgagtaaa gaagccgat aagcacata acaaatttt tgggaagct gtggggttc ataatggat gtgattctt tgcgtgaga tgtgccata gcatccatg ctttatgtt attgaagtt gttagttgg aaccacttt gtaacagtt cttgttact tatttgatt tactacgta actcggttc ttctgcttc cttctgttt cttcctctg ttaagtagg ttgggttac gggatgggt ttcaagaac atgattatt tgcgtttgg tgcggaatt aaaagtcct tttagtgtg ctgctagta gcgtctatg gcgacaata gacaaatcg aatttatat aggcatata tggttatcg atctcattt tttgttatt ggactttat actctctcc aacctaatt aatggctct ttgacgcga agtcttttg aactttatg ggctttagg actatctcc attgcaaga caagccaac atgagtaac tgcataaag catattttt gacaagcga gaaagaact attgaaata ttaaaaatg gataggttt ttggctgat agcgactgg gcattaatt agagaaacg agcgtcata aaacatcct taccatgta aatgtaaat tcaacagca gaggaagaa gaacagtat tttttggga tacaggtac acttattgt ccagagtgt aaaaaagat gttgaggaa caaccaaca gcgaaagaa ctgaacgaa ataagaaaa gaagcacaa atgagagta ctaaaagct aagaaagta tgttatacg agacaattt gaaaagggg atggtttcc aaaccagga atgcgactt ctcagtcag ggtttagaa attgaattg gacacggga gaaatatat gaagtcaat tttgatcac ctagagaaa aaatttagc ttgtgcata gatcgttac gcagataca aaattggct tttgcatac gatatatgc aaaagttat gtttcgact aacagtgaa gtcttagag atgttgcct tacttaatc gataataaa caagtaagg gatgaagtg aagatgaag ctcgatagt gatcgagtc gtcattacc aaaatgctg ggatttatt caaaaggaa aagccgtgg gtggccatt acggtcaag actaaacaa gcgattcga acgattctt agtaatatg tcagaggca ctgaatcaa cttaaagtt gccggttgg atcgacaat tttgagcaa gacaagcta ctgatggcc atggacgta ttgtatgag agagtaaac gccatcaaa atggttcaa ccaagtgcc cctaaattg atcttcaag gaggtggcg tggatggct gaggatgag cgtgtcatt aattacctt tttgaaaat gttacgctt aaaaaattt gatacggga gatatagtc tttgaagag ggcgacatt gctgactgt atctacata gtcgtaaca gggcttttt gtcgttaat tatacgcct aataaaata tgccttgaa aatttacaa gcctacggt cgattgcct attgtggac tatctttct agtacaaaa tatgacatt ccgtctgtg gactacatt gtttcaggc aattgtata ggagaactc agttttttg actgaaaga ccttacaac tgcactatt caagctgaa gcaccaagt caagttttt gtcttgaaa agtgaattt ttgagacga gcgatgaaa atgagtccc gatcccgtt attggtctg gaatgtaga atttggaag gaagttagc attagaact gcagttccg cttcttcta gcgactccc gcttatcag ttattgagc caggaacaa gtcaaatat gccttagaa cgagccttt gtaccaaat ttgagtcaa tataaaatt ttcgctgtg actgatatg attgaagat gtgataatt gtcgatggt tttgttgct gattataat acacgggag acctacctt gccccttgc tgtatttct agaactgta cagaaactt attttgccc tcaagcagc tatatgaat gtttcattt gatgttgaa acgaagctt ttaattatt ccagatcaa gaagtagat gaatatgat gttatgatg atggctgaa gaaacttgc gaaatgaat actcgcata ccaatcccc tatcaagtc tttatgttg gcgagtgca tcggtatgg ggagttatc atgtactac aatgaggtg cctttgctc ttttcaaag gcaatagat gtacccatg gtagatgtc gcgctcctt tacgttcct gccgcaata tttaatata tctttctgc atggacgtc cctattttt tttaaaagt atcccacaa attttgatc ataggactg cctgtggct ggattaacg ggtattttt aacggaatt ttgatgaga cagctgata aatgacaaa tggccatta gcgtttggc ttttttttt ggacttctc tgtatacca acgaatccc aaatttatt ggctacctc cttaaggaa cagtcatta cgaacgaaa cacattggc gttctttta gaaggggaa gcattgatt agttttgta ttgggagat tatctatac ttactaaca gtatcatat atgtcgaaa catgtggta cattggtat caaattatt agtgtgata ctccgtttt gttgttatg ggtatcttg gagggactc ttgttcggg atgatgggt cgttatctc gtgcgaaaa atgtcatgg aacaagttg agcatgttt tcaatattt ttcgtgatg ccgtttttg tgtttttcc ttcgctttt aactttggc tccggttgt ggcacgaca ggagtagcg atactcggt cttataatg ggcctcgag cgaacaact ctgagtaga gaagctgaa aaatacttg acaaatttt tgggaaggt ttgggattc ataatggac gtgcttttg tgtgtaagg tgtgccgtt gatgcaatg atttatgtt gtcggaaaa gttgaatgg agtcactat gttctaatt ttggttgtt tatttggta tactacgta actcggttt tcttgctat cttctgttt ctgccatta ctaagtaga ctgggttat ggaatggat tttaaaaac atgatgatt tgtgtatgg tgtggaatc aaaaatcct ctcagtttg ttgatggta gcatcgatg gcagtcata gacgtatca agatcatac gacgagata tggagattg tgttttttt ttctttctt ggtttatac actttgtcc aacctaatc aatggctct ttgacgcgc aatcttttg aatttcatg ggttttcgg acaatttcc atgtcaaga caagccaac atgagcaac tgtataaaa catattttt aacaagagg caacgaaca attgatata ttaaaaatg gataggttt ttggctgat aacgactgg tcattaatt agaaaaata tgtaccata gaacatccc tatcatata gatttaacg acagaggaa gaagaacaa ttatttctg ggatacaga tacacttat tgtccagaa tgtaaaaag gatgttgag gaacaaccc actgcgaaa gaaattaaa gaaataatg aaagaagca caaatgaga gtactaaaa gctaaaaaa atatgttac accagacaa tttgagagg gggatggtt tccaaacca ggaatgcga cttctcagt caggcttta gaagttgaa ctagacacg ggagaaata tatcaagtt aatttagac aacgttgag aaacaattc agactgaat atatgtcaa cgcctcttt cggaagctt cttcttcga attcaacgt ttgcgcaca agttgtttg agaccaaaa acttactgg agacgagtc tgctactat ctcgtcata aaaacctcg tacttggat atattaatg ttcgtcatt gtcattctt aatattgtc gtcattgga attgattgg cgttatcat tctactttt acaacagat agtacacac ctcgaacct tcccataaa ctaaattac gttataacg acaataaaa ctgactttt atttttgtt tatttttcg gaattcata ataaaggtg atggcatac tctcgtaga agtatttgt gccgacggc ctgaagaga tatttcaaa tcccttgca aatactatt gatgtaatg atcattttt atactgatt gttaatgta actttcgtt actaacgaa actgttaat ctatcacac aaccacgaa agatctgaa gaagaagaa actgtccac ttagtattc agttggtta cagttgctt gtaattgtg aaactagct gtggtttta cgaattttt aatcctctt ttgttgaat tgcttggaa cgcaaggcg gacgcaaaa atggctttg gcatatgat atatgcaaa agctacgct atgagcaac agcgaagtc ttagaaatg ttgccttat ttaatcgac aataaacaa gtaagagaa gaagtgaag atgaaactg aactgtgat cgagtgctt attactaaa atgttaggg cttattcaa aaagagaag ccttgggtg gccattacg gtcaagact aagcaagcg attcgaacg attcttagc aacatgtca gaggctgtg aatcatctc cgagttggc ggttggatc gataatttc gagcaggaa aaactgctg ttggcaatg gatgtttta tatgacaga gtaaacgcc atcgaaatg gttcagcca agcgcccct aaggtaatc ttcaaggag gtggcgtgg atggctgag gatgaagat gttattaat tttcttttt gaaaatgtt acggttaaa aaatttgat accggggat gtagttttt gatgagggt gacgttgct gatggtatt tacatagtc gtaacaggt tga
- >TC033683 RA
gaccttaaa attatgatc tatttcagt gggtgtact tacgggtgt tatttgcgg aattttgat gaagtatct ggtaaacga gagttggtt tttattgtt tggcatttt ttttggagt tctctgtat accgagtaa tcccaaatt tgttgggta tctccttaa agaacagtc attgcacac aaaacacat cgcagtcct cttagaagg tgaggcatt gattacatt tgttgtggg agattattg ttacttcct actaacatc ttacatggc gggatacgt ggaacattg gtaccagat gattagtgt gttactccg ttttcttgt tgtcggtac tttggaagg agtcttatt tgggttagc cggtcgttt tctcgtgcg aaaaatgtc atggaacaa gttgagcat gttttcaat atttttcgt gatgccgtt tttgtgttt ttctttcgc atttaactt tggctccgg ttctggcac gacgggggt agcgatcct cggtcttat attgggtct cgaacgaac aactttgag taaagaagc cgataagca cataacaaa tttttggga agctgtggg gttcataat ggatgtgat tctttgcgt gagatgtgc catagcatc catgcttta tgttattga agttgttag ttggaacca ctttgtaac agttcttgt tacttattt gatttacta cgtaactcg gttcttctg cttccttct gtttcttcc tctgttaag taggttggg ttacgggat gggtttcaa gaacatgat tatttgcgt ttggtgcgg aattaaaag tccttttag tgtgctgct agtagcgtc tatggcgac aatagacaa atcgaattt atataggca tatatggtt atcgatctc attttttgt tattggact ttatactct ctccaacct aattaatgg ctctttgac gcgaagtct tttgaactt tatgggctt taggactat ctccattgc aagacaagc caacatgag taactgcat aaagcatat ttttgacaa gcgagaaag aactattga aatattaaa aatggatag gtttttggc tgatagcga ctgggcatt aattagaga aacgagcgt cataaaaca tccttacca tgtaaatgt aaattcaac agcagagga agaagaaca gtatttttt gggatacag gtacactta ttgtccaga gtgtaaaaa agatgttga ggaacaacc aacagcgaa agaactgaa cgaaataag aaaagaagc acaaatgag agtactaaa agctaagaa agtatgtta tacgagaca atttgaaaa ggggatggt ttccaaacc aggaatgcg acttctcag tcagggttt agaaattga attggacac gggagaaat atatgaagt caattttga tcacctaga gaaaaaatt tagcttgtg catagatcg ttacgcaga tacaaaatt ggcttttgc atacgatat atgcaaaag ttatgtttc gactaacag tgaagtctt agagatgtt gccttactt aatcgataa taaacaagt aagggatga agtgaagat gaagctcga tagtgatcg agtcgtcat taccaaaat gctgggatt tattcaaaa ggaaaagcc gtgggtggc cattacggt caagactaa acaagcgat tcgaacgat tcttagtaa tatgtcaga ggcactgaa tcaacttaa agttgccgg ttggatcga caattttga gcaagacaa gctactgat ggccatgga cgtattgta tgagagagt aaacgccat caaaatggt tcaaccaag tgcccctaa attgatctt caaggaggt ggcgtggat ggctgagga tgagcgtgt cattaatta cctttttga aaatgttac gcttaaaaa atttgatac gggagatat agtctttga agagggcga cattgctga ctgtatcta catagtcgt aacagggct ttttgtcgt taattatac gcctaataa aatatgcct tgaaaattt acaagccta cggtcgatt gcctattgt ggactatct ttctagtac aaaatatga cattccgtc tgtggacta cattgtttc aggcaattg tataggaga actcagttt tttgactga aagacctta caactgcac tattcaagc tgaagcacc aagtcaagt ttttgtctt gaaaagtga atttttgag acgagcgat gaaaatgag tcccgatcc cgttattgg tctggaatg tagaatttg gaaggaagt tagcattag aactgcagt tccgcttct tctagcgac tcccgctta tcagttatt gagccagga acaagtcaa atatgcctt agaacgagc ctttgtacc aaatttgag tcaatataa aattttcgc tgtgactga tatgattga agatgtgat aattgtcga tggttttgt tgctgatta taatacacg ggagaccta ccttgcccc ttgctgtat ttctagaac tgtacagaa acttatttt gccctcaag cagctatat gaatgtttc atttgatgt tgaaacgaa gcttttaat tattccaga tcaagaagt agatgaata tgatgttat gatgatggc tgaagaaac ttgcgaaat gaatactcg cataccaat cccctatca agtctttat gttggcgag tgcatcggt atggggagt tatcatgta ctacaatga ggtgccttt gctcttttc aaaggcaat agatgtacc catggtaga tgtcgcgct cctttacgt tcctgccgc aatatttaa tatatcttt ctgcatgga cgtccctat tttttttaa aagtatccc acaaatttt gatcatagg actgcctgt ggctggatt aacgggtat ttttaacgg aattttgat gagacagct gataaatga caaatggcc attagcgtt tggcttttt ttttggact tctctgtat accaacgaa tcccaaatt tattggcta cctccttaa ggaacagtc attacgaac gaaacacat tggcgttct tttagaagg ggaagcatt gattagttt tgtattggg agattatct atacttact aacagtatc atatatgtc gaaacatgt ggtacattg gtatcaaat tattagtgt gatactccg ttttgttgt tatgggtat cttggaggg actcttgtt cgggatgat gggtcgtta tctcgtgcg aaaaatgtc atggaacaa gttgagcat gttttcaat atttttcgt gatgccgtt tttgtgttt ttccttcgc ttttaactt tggctccgg ttgtggcac gacaggagt agcgatact cggtcttat aatgggcct cgagcgaac aactctgag tagagaagc tgaaaaata cttgacaaa tttttggga aggtttggg attcataat ggacgtgct tttgtgtgt aaggtgtgc cgttgatgc aatgattta tgttgtcgg aaaagttga atggagtca ctatgttct aattttggt tgtttattt ggtatacta cgtaactcg gttttcttg ctatcttct gtttctgcc attactaag tagactggg ttatggaat ggattttaa aaacatgat gatttgtgt atggtgtgg aatcaaaaa tcctctcag tttgttgat ggtagcatc gatggcagt catagacgt atcaagatc atacgacga gatatggag attgtgttt ttttttctt tcttggttt atacacttt gtccaacct aatcaatgg ctctttgac gcgcaatct tttgaattt catgggttt tcggacaat ttccatgtc aagacaagc caacatgag caactgtat aaaacatat ttttaacaa gaggcaacg aacaattga tatattaaa aatggatag gtttttggc tgataacga ctggtcatt aattagaaa aatatgtac catagaaca tccctatca tatagattt aacgacaga ggaagaaga acaattatt tctgggata cagatacac ttattgtcc agaatgtaa aaaggatgt tgaggaaca acccactgc gaaagaaat taaagaaat aatgaaaga agcacaaat gagagtact aaaagctaa aaaaatatg ttacaccag acaatttga gagggggat ggtttccaa accaggaat gcgacttct cagtcaggc tttagaagt tgaactaga cacgggaga aatatatca agttaattt agacaacgt tgagaaaca attcagact gaatatatg tcaacgcct ctttcggaa gcttcttct tcgaattca acgtttgcg cacaagttg tttgagacc aaaaactta ctggagacg agtctgcta ctatctcgt cataaaaac ctcgtactt ggatatatt aatgttcgt cattgtcat tcttaatat tgtcgtcat tggaattga ttggcgtta tcattctac ttttacaac agatagtac acacctcga accttccca taaactaaa ttacgttat aacgacaat aaaactgac ttttatttt tgtttattt ttcggaatt cataataaa ggtgatggc atactctcg tagaagtat ttgtgccga cggcctgaa gagatattt caaatccct tgcaaatac tattgatgt aatgatcat ttttatact gattgttaa tgtaacttt cgttactaa cgaaactgt taatctatc acacaacca cgaaagatc tgaagaaga agaaactgt ccacttagt attcagttg gttacagtt gcttgtaat tgtgaaact agctgtggt tttacgaat ttttaatcc tcttttgtt gaattgctt ggaacgcaa ggcggacgc aaaaatggc tttggcata tgatatatg caaaagcta cgctatgag caacagcga agtcttaga aatgttgcc ttatttaat cgacaataa acaagtaag agaagaagt gaagatgaa actgaactg tgatcgagt gcttattac taaaatgtt agggcttat tcaaaaaga gaagccttg ggtggccat tacggtcaa gactaagca agcgattcg aacgattct tagcaacat gtcagaggc tgtgaatca tctccgagt tggcggttg gatcgataa tttcgagca ggaaaaact gctgttggc aatggatgt tttatatga cagagtaaa cgccatcga aatggttca gccaagcgc ccctaaggt aatcttcaa ggaggtggc gtggatggc tgaggatga agatgttat taattttct ttttgaaaa tgttacggt taaaaaatt tgataccgg ggatgtagt ttttgatga gggtgacgt tgctgatgg tatttacat agtcgtaac aggttgact gtatacttt tctaaagcg catttgaga aaaatattt tttaaggac ttttcgtta taacttaca agcctaata aaaattgtc ttgaaag