Status
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Gene information
General information
TC033683
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009756,TC009755
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7389411..7397337
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033683
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02851

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033683 RA
        MAGYVEHWY QMISVLLRF LVVGTLEGV LFGLAGRFL 
        VRKMSWNKL SMFSIFFVM PFLCFSFAF NFGSGSGTT 
        GVAILGLIL GLERTTLSK EADKHITNF WEAVGFIMD 
        VILCVRCAI ASMLYVIEV VSWNHFVTV LVTYLIYYV 
        TRFFCFLLF LPLLSRLGY GMGFKNMII CVWCGIKSP 
        FSVLLVASM ATIDKSNLY RHIWLSISF FVIGLYTLS 
        NLINGSLTR SLLNFMGFR TISIARQAN MSNCIKHIF 
        DKRERTIEI LKMDRFLAD SDWALIRET SVIKHPYHV 
        NVNSTAEEE EQYFLGYRY TYCPECKKD VEEQPTAKE 
        LNEIRKEAQ MRVLKAKKV CYTRQFEKG MVSKPGMRL 
        LSQGLEIEL DTGEIYEVN FDHLEKKFS LCIDRYADT 
        KLAFAYDIC KSYVSTNSE VLEMLPYLI DNKQVRDEV 
        KMKLDSDRV VITKMLGFI QKEKPWVAI TVKTKQAIR 
        TILSNMSEA LNQLKVAGW IDNFEQDKL LMAMDVLYE 
        RVNAIKMVQ PSAPKLIFK EVAWMAEDE RVINYLFEN 
        VTLKKFDTG DIVFEEGDI ADCIYIVVT GLFVVNYTP 
        NKICLENLQ AYGRLPIVD YLSSTKYDI PSVDYIVSG 
        NCIGELSFL TERPYNCTI QAEAPSQVF VLKSEFLRR 
        AMKMSPDPV IGLECRIWK EVSIRTAVP LLLATPAYQ 
        LLSQEQVKY ALERAFVPN LSQYKIFAV TDMIEDVII 
        VDGFVADYN TRETYLAPC CISRTVQKL ILPSSSYMN 
        VSFDVETKL LIIPDQEVD EYDVMMMAE ETCEMNTRI 
        PIPYQVFML ASASVWGVI MYYNEVPLL FSKAIDVPM 
        VDVALLYVP AAIFNISFC MDVPIFFKS IPQILIIGL 
        PVAGLTGIF NGILMRQLI NDKWPLAFG FFFGLLCIP 
        TNPKFIGYL LKEQSLRTK HIGVLLEGE ALISFVLGD 
        YLYLLTVSY MSKHVVHWY QIISVILRF VVMGILEGL 
        LFGMMGRYL VRKMSWNKL SMFSIFFVM PFLCFSFAF 
        NFGSGCGTT GVAILGLIM GLERTTLSR EAEKYLTNF 
        WEGLGFIMD VLLCVRCAV DAMIYVVGK VEWSHYVLI 
        LVVYLVYYV TRFSCYLLF LPLLSRLGY GMDFKNMMI 
        CVWCGIKNP LSLLMVASM AVIDVSRSY DEIWRLCFF 
        FFLGLYTLS NLINGSLTR NLLNFMGFR TISMSRQAN 
        MSNCIKHIF NKRQRTIDI LKMDRFLAD NDWSLIRKI 
        CTIEHPYHI DLTTEEEEQ LFLGYRYTY CPECKKDVE 
        EQPTAKEIK EIMKEAQMR VLKAKKICY TRQFERGMV 
        SKPGMRLLS QALEVELDT GEIYQVNLD NVEKQFRLN 
        ICQRLFRKL LLRIQRLRT SCLRPKTYW RRVCYYLVI 
        KTSYLDILM FVIVILNIV VIGIDWRYH STFTTDSTH 
        LEPSHKLNY VITTIKLTF IFVYFSEFI IKVMAYSRR 
        SICADGLKR YFKSLANTI DVMIIFILI VNVTFVTNE 
        TVNLSHNHE RSEEEETVH LVFSWLQLL VIVKLAVVL 
        RIFNPLLLN CLERKADAK MALAYDICK SYAMSNSEV 
        LEMLPYLID NKQVREEVK MKLNCDRVL ITKMLGLIQ 
        KEKPWVAIT VKTKQAIRT ILSNMSEAV NHLRVGGWI 
        DNFEQEKLL LAMDVLYDR VNAIEMVQP SAPKVIFKE 
        VAWMAEDED VINFLFENV TVKKFDTGD VVFDEGDVA 
        DGIYIVVTG
      CDS sequence
      • >TC033683 RA
        atggcggga tacgtggaa cattggtac cagatgatt 
        agtgtgtta ctccgtttt cttgttgtc ggtactttg 
        gaaggagtc ttatttggg ttagccggt cgttttctc 
        gtgcgaaaa atgtcatgg aacaagttg agcatgttt 
        tcaatattt ttcgtgatg ccgtttttg tgtttttct 
        ttcgcattt aactttggc tccggttct ggcacgacg 
        ggggtagcg atcctcggt cttatattg ggtctcgaa 
        cgaacaact ttgagtaaa gaagccgat aagcacata 
        acaaatttt tgggaagct gtggggttc ataatggat 
        gtgattctt tgcgtgaga tgtgccata gcatccatg 
        ctttatgtt attgaagtt gttagttgg aaccacttt 
        gtaacagtt cttgttact tatttgatt tactacgta 
        actcggttc ttctgcttc cttctgttt cttcctctg 
        ttaagtagg ttgggttac gggatgggt ttcaagaac 
        atgattatt tgcgtttgg tgcggaatt aaaagtcct 
        tttagtgtg ctgctagta gcgtctatg gcgacaata 
        gacaaatcg aatttatat aggcatata tggttatcg 
        atctcattt tttgttatt ggactttat actctctcc 
        aacctaatt aatggctct ttgacgcga agtcttttg 
        aactttatg ggctttagg actatctcc attgcaaga 
        caagccaac atgagtaac tgcataaag catattttt 
        gacaagcga gaaagaact attgaaata ttaaaaatg 
        gataggttt ttggctgat agcgactgg gcattaatt 
        agagaaacg agcgtcata aaacatcct taccatgta 
        aatgtaaat tcaacagca gaggaagaa gaacagtat 
        tttttggga tacaggtac acttattgt ccagagtgt 
        aaaaaagat gttgaggaa caaccaaca gcgaaagaa 
        ctgaacgaa ataagaaaa gaagcacaa atgagagta 
        ctaaaagct aagaaagta tgttatacg agacaattt 
        gaaaagggg atggtttcc aaaccagga atgcgactt 
        ctcagtcag ggtttagaa attgaattg gacacggga 
        gaaatatat gaagtcaat tttgatcac ctagagaaa 
        aaatttagc ttgtgcata gatcgttac gcagataca 
        aaattggct tttgcatac gatatatgc aaaagttat 
        gtttcgact aacagtgaa gtcttagag atgttgcct 
        tacttaatc gataataaa caagtaagg gatgaagtg 
        aagatgaag ctcgatagt gatcgagtc gtcattacc 
        aaaatgctg ggatttatt caaaaggaa aagccgtgg 
        gtggccatt acggtcaag actaaacaa gcgattcga 
        acgattctt agtaatatg tcagaggca ctgaatcaa 
        cttaaagtt gccggttgg atcgacaat tttgagcaa 
        gacaagcta ctgatggcc atggacgta ttgtatgag 
        agagtaaac gccatcaaa atggttcaa ccaagtgcc 
        cctaaattg atcttcaag gaggtggcg tggatggct 
        gaggatgag cgtgtcatt aattacctt tttgaaaat 
        gttacgctt aaaaaattt gatacggga gatatagtc 
        tttgaagag ggcgacatt gctgactgt atctacata 
        gtcgtaaca gggcttttt gtcgttaat tatacgcct 
        aataaaata tgccttgaa aatttacaa gcctacggt 
        cgattgcct attgtggac tatctttct agtacaaaa 
        tatgacatt ccgtctgtg gactacatt gtttcaggc 
        aattgtata ggagaactc agttttttg actgaaaga 
        ccttacaac tgcactatt caagctgaa gcaccaagt 
        caagttttt gtcttgaaa agtgaattt ttgagacga 
        gcgatgaaa atgagtccc gatcccgtt attggtctg 
        gaatgtaga atttggaag gaagttagc attagaact 
        gcagttccg cttcttcta gcgactccc gcttatcag 
        ttattgagc caggaacaa gtcaaatat gccttagaa 
        cgagccttt gtaccaaat ttgagtcaa tataaaatt 
        ttcgctgtg actgatatg attgaagat gtgataatt 
        gtcgatggt tttgttgct gattataat acacgggag 
        acctacctt gccccttgc tgtatttct agaactgta 
        cagaaactt attttgccc tcaagcagc tatatgaat 
        gtttcattt gatgttgaa acgaagctt ttaattatt 
        ccagatcaa gaagtagat gaatatgat gttatgatg 
        atggctgaa gaaacttgc gaaatgaat actcgcata 
        ccaatcccc tatcaagtc tttatgttg gcgagtgca 
        tcggtatgg ggagttatc atgtactac aatgaggtg 
        cctttgctc ttttcaaag gcaatagat gtacccatg 
        gtagatgtc gcgctcctt tacgttcct gccgcaata 
        tttaatata tctttctgc atggacgtc cctattttt 
        tttaaaagt atcccacaa attttgatc ataggactg 
        cctgtggct ggattaacg ggtattttt aacggaatt 
        ttgatgaga cagctgata aatgacaaa tggccatta 
        gcgtttggc ttttttttt ggacttctc tgtatacca 
        acgaatccc aaatttatt ggctacctc cttaaggaa 
        cagtcatta cgaacgaaa cacattggc gttctttta 
        gaaggggaa gcattgatt agttttgta ttgggagat 
        tatctatac ttactaaca gtatcatat atgtcgaaa 
        catgtggta cattggtat caaattatt agtgtgata 
        ctccgtttt gttgttatg ggtatcttg gagggactc 
        ttgttcggg atgatgggt cgttatctc gtgcgaaaa 
        atgtcatgg aacaagttg agcatgttt tcaatattt 
        ttcgtgatg ccgtttttg tgtttttcc ttcgctttt 
        aactttggc tccggttgt ggcacgaca ggagtagcg 
        atactcggt cttataatg ggcctcgag cgaacaact 
        ctgagtaga gaagctgaa aaatacttg acaaatttt 
        tgggaaggt ttgggattc ataatggac gtgcttttg 
        tgtgtaagg tgtgccgtt gatgcaatg atttatgtt 
        gtcggaaaa gttgaatgg agtcactat gttctaatt 
        ttggttgtt tatttggta tactacgta actcggttt 
        tcttgctat cttctgttt ctgccatta ctaagtaga 
        ctgggttat ggaatggat tttaaaaac atgatgatt 
        tgtgtatgg tgtggaatc aaaaatcct ctcagtttg 
        ttgatggta gcatcgatg gcagtcata gacgtatca 
        agatcatac gacgagata tggagattg tgttttttt 
        ttctttctt ggtttatac actttgtcc aacctaatc 
        aatggctct ttgacgcgc aatcttttg aatttcatg 
        ggttttcgg acaatttcc atgtcaaga caagccaac 
        atgagcaac tgtataaaa catattttt aacaagagg 
        caacgaaca attgatata ttaaaaatg gataggttt 
        ttggctgat aacgactgg tcattaatt agaaaaata 
        tgtaccata gaacatccc tatcatata gatttaacg 
        acagaggaa gaagaacaa ttatttctg ggatacaga 
        tacacttat tgtccagaa tgtaaaaag gatgttgag 
        gaacaaccc actgcgaaa gaaattaaa gaaataatg 
        aaagaagca caaatgaga gtactaaaa gctaaaaaa 
        atatgttac accagacaa tttgagagg gggatggtt 
        tccaaacca ggaatgcga cttctcagt caggcttta 
        gaagttgaa ctagacacg ggagaaata tatcaagtt 
        aatttagac aacgttgag aaacaattc agactgaat 
        atatgtcaa cgcctcttt cggaagctt cttcttcga 
        attcaacgt ttgcgcaca agttgtttg agaccaaaa 
        acttactgg agacgagtc tgctactat ctcgtcata 
        aaaacctcg tacttggat atattaatg ttcgtcatt 
        gtcattctt aatattgtc gtcattgga attgattgg 
        cgttatcat tctactttt acaacagat agtacacac 
        ctcgaacct tcccataaa ctaaattac gttataacg 
        acaataaaa ctgactttt atttttgtt tatttttcg 
        gaattcata ataaaggtg atggcatac tctcgtaga 
        agtatttgt gccgacggc ctgaagaga tatttcaaa 
        tcccttgca aatactatt gatgtaatg atcattttt 
        atactgatt gttaatgta actttcgtt actaacgaa 
        actgttaat ctatcacac aaccacgaa agatctgaa 
        gaagaagaa actgtccac ttagtattc agttggtta 
        cagttgctt gtaattgtg aaactagct gtggtttta 
        cgaattttt aatcctctt ttgttgaat tgcttggaa 
        cgcaaggcg gacgcaaaa atggctttg gcatatgat 
        atatgcaaa agctacgct atgagcaac agcgaagtc 
        ttagaaatg ttgccttat ttaatcgac aataaacaa 
        gtaagagaa gaagtgaag atgaaactg aactgtgat 
        cgagtgctt attactaaa atgttaggg cttattcaa 
        aaagagaag ccttgggtg gccattacg gtcaagact 
        aagcaagcg attcgaacg attcttagc aacatgtca 
        gaggctgtg aatcatctc cgagttggc ggttggatc 
        gataatttc gagcaggaa aaactgctg ttggcaatg 
        gatgtttta tatgacaga gtaaacgcc atcgaaatg 
        gttcagcca agcgcccct aaggtaatc ttcaaggag 
        gtggcgtgg atggctgag gatgaagat gttattaat 
        tttcttttt gaaaatgtt acggttaaa aaatttgat 
        accggggat gtagttttt gatgagggt gacgttgct 
        gatggtatt tacatagtc gtaacaggt tga
      mRNA sequence
      • >TC033683 RA
        gaccttaaa attatgatc tatttcagt gggtgtact 
        tacgggtgt tatttgcgg aattttgat gaagtatct 
        ggtaaacga gagttggtt tttattgtt tggcatttt 
        ttttggagt tctctgtat accgagtaa tcccaaatt 
        tgttgggta tctccttaa agaacagtc attgcacac 
        aaaacacat cgcagtcct cttagaagg tgaggcatt 
        gattacatt tgttgtggg agattattg ttacttcct 
        actaacatc ttacatggc gggatacgt ggaacattg 
        gtaccagat gattagtgt gttactccg ttttcttgt 
        tgtcggtac tttggaagg agtcttatt tgggttagc 
        cggtcgttt tctcgtgcg aaaaatgtc atggaacaa 
        gttgagcat gttttcaat atttttcgt gatgccgtt 
        tttgtgttt ttctttcgc atttaactt tggctccgg 
        ttctggcac gacgggggt agcgatcct cggtcttat 
        attgggtct cgaacgaac aactttgag taaagaagc 
        cgataagca cataacaaa tttttggga agctgtggg 
        gttcataat ggatgtgat tctttgcgt gagatgtgc 
        catagcatc catgcttta tgttattga agttgttag 
        ttggaacca ctttgtaac agttcttgt tacttattt 
        gatttacta cgtaactcg gttcttctg cttccttct 
        gtttcttcc tctgttaag taggttggg ttacgggat 
        gggtttcaa gaacatgat tatttgcgt ttggtgcgg 
        aattaaaag tccttttag tgtgctgct agtagcgtc 
        tatggcgac aatagacaa atcgaattt atataggca 
        tatatggtt atcgatctc attttttgt tattggact 
        ttatactct ctccaacct aattaatgg ctctttgac 
        gcgaagtct tttgaactt tatgggctt taggactat 
        ctccattgc aagacaagc caacatgag taactgcat 
        aaagcatat ttttgacaa gcgagaaag aactattga 
        aatattaaa aatggatag gtttttggc tgatagcga 
        ctgggcatt aattagaga aacgagcgt cataaaaca 
        tccttacca tgtaaatgt aaattcaac agcagagga 
        agaagaaca gtatttttt gggatacag gtacactta 
        ttgtccaga gtgtaaaaa agatgttga ggaacaacc 
        aacagcgaa agaactgaa cgaaataag aaaagaagc 
        acaaatgag agtactaaa agctaagaa agtatgtta 
        tacgagaca atttgaaaa ggggatggt ttccaaacc 
        aggaatgcg acttctcag tcagggttt agaaattga 
        attggacac gggagaaat atatgaagt caattttga 
        tcacctaga gaaaaaatt tagcttgtg catagatcg 
        ttacgcaga tacaaaatt ggcttttgc atacgatat 
        atgcaaaag ttatgtttc gactaacag tgaagtctt 
        agagatgtt gccttactt aatcgataa taaacaagt 
        aagggatga agtgaagat gaagctcga tagtgatcg 
        agtcgtcat taccaaaat gctgggatt tattcaaaa 
        ggaaaagcc gtgggtggc cattacggt caagactaa 
        acaagcgat tcgaacgat tcttagtaa tatgtcaga 
        ggcactgaa tcaacttaa agttgccgg ttggatcga 
        caattttga gcaagacaa gctactgat ggccatgga 
        cgtattgta tgagagagt aaacgccat caaaatggt 
        tcaaccaag tgcccctaa attgatctt caaggaggt 
        ggcgtggat ggctgagga tgagcgtgt cattaatta 
        cctttttga aaatgttac gcttaaaaa atttgatac 
        gggagatat agtctttga agagggcga cattgctga 
        ctgtatcta catagtcgt aacagggct ttttgtcgt 
        taattatac gcctaataa aatatgcct tgaaaattt 
        acaagccta cggtcgatt gcctattgt ggactatct 
        ttctagtac aaaatatga cattccgtc tgtggacta 
        cattgtttc aggcaattg tataggaga actcagttt 
        tttgactga aagacctta caactgcac tattcaagc 
        tgaagcacc aagtcaagt ttttgtctt gaaaagtga 
        atttttgag acgagcgat gaaaatgag tcccgatcc 
        cgttattgg tctggaatg tagaatttg gaaggaagt 
        tagcattag aactgcagt tccgcttct tctagcgac 
        tcccgctta tcagttatt gagccagga acaagtcaa 
        atatgcctt agaacgagc ctttgtacc aaatttgag 
        tcaatataa aattttcgc tgtgactga tatgattga 
        agatgtgat aattgtcga tggttttgt tgctgatta 
        taatacacg ggagaccta ccttgcccc ttgctgtat 
        ttctagaac tgtacagaa acttatttt gccctcaag 
        cagctatat gaatgtttc atttgatgt tgaaacgaa 
        gcttttaat tattccaga tcaagaagt agatgaata 
        tgatgttat gatgatggc tgaagaaac ttgcgaaat 
        gaatactcg cataccaat cccctatca agtctttat 
        gttggcgag tgcatcggt atggggagt tatcatgta 
        ctacaatga ggtgccttt gctcttttc aaaggcaat 
        agatgtacc catggtaga tgtcgcgct cctttacgt 
        tcctgccgc aatatttaa tatatcttt ctgcatgga 
        cgtccctat tttttttaa aagtatccc acaaatttt 
        gatcatagg actgcctgt ggctggatt aacgggtat 
        ttttaacgg aattttgat gagacagct gataaatga 
        caaatggcc attagcgtt tggcttttt ttttggact 
        tctctgtat accaacgaa tcccaaatt tattggcta 
        cctccttaa ggaacagtc attacgaac gaaacacat 
        tggcgttct tttagaagg ggaagcatt gattagttt 
        tgtattggg agattatct atacttact aacagtatc 
        atatatgtc gaaacatgt ggtacattg gtatcaaat 
        tattagtgt gatactccg ttttgttgt tatgggtat 
        cttggaggg actcttgtt cgggatgat gggtcgtta 
        tctcgtgcg aaaaatgtc atggaacaa gttgagcat 
        gttttcaat atttttcgt gatgccgtt tttgtgttt 
        ttccttcgc ttttaactt tggctccgg ttgtggcac 
        gacaggagt agcgatact cggtcttat aatgggcct 
        cgagcgaac aactctgag tagagaagc tgaaaaata 
        cttgacaaa tttttggga aggtttggg attcataat 
        ggacgtgct tttgtgtgt aaggtgtgc cgttgatgc 
        aatgattta tgttgtcgg aaaagttga atggagtca 
        ctatgttct aattttggt tgtttattt ggtatacta 
        cgtaactcg gttttcttg ctatcttct gtttctgcc 
        attactaag tagactggg ttatggaat ggattttaa 
        aaacatgat gatttgtgt atggtgtgg aatcaaaaa 
        tcctctcag tttgttgat ggtagcatc gatggcagt 
        catagacgt atcaagatc atacgacga gatatggag 
        attgtgttt ttttttctt tcttggttt atacacttt 
        gtccaacct aatcaatgg ctctttgac gcgcaatct 
        tttgaattt catgggttt tcggacaat ttccatgtc 
        aagacaagc caacatgag caactgtat aaaacatat 
        ttttaacaa gaggcaacg aacaattga tatattaaa 
        aatggatag gtttttggc tgataacga ctggtcatt 
        aattagaaa aatatgtac catagaaca tccctatca 
        tatagattt aacgacaga ggaagaaga acaattatt 
        tctgggata cagatacac ttattgtcc agaatgtaa 
        aaaggatgt tgaggaaca acccactgc gaaagaaat 
        taaagaaat aatgaaaga agcacaaat gagagtact 
        aaaagctaa aaaaatatg ttacaccag acaatttga 
        gagggggat ggtttccaa accaggaat gcgacttct 
        cagtcaggc tttagaagt tgaactaga cacgggaga 
        aatatatca agttaattt agacaacgt tgagaaaca 
        attcagact gaatatatg tcaacgcct ctttcggaa 
        gcttcttct tcgaattca acgtttgcg cacaagttg 
        tttgagacc aaaaactta ctggagacg agtctgcta 
        ctatctcgt cataaaaac ctcgtactt ggatatatt 
        aatgttcgt cattgtcat tcttaatat tgtcgtcat 
        tggaattga ttggcgtta tcattctac ttttacaac 
        agatagtac acacctcga accttccca taaactaaa 
        ttacgttat aacgacaat aaaactgac ttttatttt 
        tgtttattt ttcggaatt cataataaa ggtgatggc 
        atactctcg tagaagtat ttgtgccga cggcctgaa 
        gagatattt caaatccct tgcaaatac tattgatgt 
        aatgatcat ttttatact gattgttaa tgtaacttt 
        cgttactaa cgaaactgt taatctatc acacaacca 
        cgaaagatc tgaagaaga agaaactgt ccacttagt 
        attcagttg gttacagtt gcttgtaat tgtgaaact 
        agctgtggt tttacgaat ttttaatcc tcttttgtt 
        gaattgctt ggaacgcaa ggcggacgc aaaaatggc 
        tttggcata tgatatatg caaaagcta cgctatgag 
        caacagcga agtcttaga aatgttgcc ttatttaat 
        cgacaataa acaagtaag agaagaagt gaagatgaa 
        actgaactg tgatcgagt gcttattac taaaatgtt 
        agggcttat tcaaaaaga gaagccttg ggtggccat 
        tacggtcaa gactaagca agcgattcg aacgattct 
        tagcaacat gtcagaggc tgtgaatca tctccgagt 
        tggcggttg gatcgataa tttcgagca ggaaaaact 
        gctgttggc aatggatgt tttatatga cagagtaaa 
        cgccatcga aatggttca gccaagcgc ccctaaggt 
        aatcttcaa ggaggtggc gtggatggc tgaggatga 
        agatgttat taattttct ttttgaaaa tgttacggt 
        taaaaaatt tgataccgg ggatgtagt ttttgatga 
        gggtgacgt tgctgatgg tatttacat agtcgtaac 
        aggttgact gtatacttt tctaaagcg catttgaga 
        aaaatattt tttaaggac ttttcgtta taacttaca 
        agcctaata aaaattgtc ttgaaag
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