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Gene information
General information
TC009743 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7171381..7187105
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009743
    iBeetle screen iB_02849

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009743 RA
      MAYAISPAG PVYTGPGAS PTQATGEPF AADSPYFPF 
      SARPGSRLS PAKPKAGLR PTKEEPPQG NSIDNQWYG 
      SDEFNDSPR YTSPNPTTG YYLGADATP GEWHPSYTT 
      GAYQTYEAY GLIPETEGQ GLPPMSSLR PNGTAVTTM 
      SGTNSSAFG PANHTDGVN KALGTIYPI VEQASASSY 
      SSAPSTPVS SPPPLAPSS WLANHNTPH SPHYTQTVN 
      VNNSAAPQG LHMPPAPEA QRLDDAIGF LRDHAEVNG 
      TRMEERLDD AINVLRNHA EPQIIGQLP PPHQMTTLG 
      YTAAASEPH IPDTVKVER ATYNTKKRK EPPDSDTKP 
      SSSVETIVQ PNSTSGPPP KTTKRSRRY NGDEDEIDD 
      PSNKAVREK ERRQANNVR ERIRIRDIN EALKELGRM 
      CMAHLKTDK PQTKLGILN MAVEVIMTL EQQVRERNL 
      NPKAACLKR REEEKAEDG PKLGPGPHH MLTAQHYPT 
      LPGAPANLP HNPGQPQ
    • >TC009743 RB
      MAASDEEPM HHLYEVFTN CFNKIANKA PEKPGFQSP 
      YGGIDNGMA YAISPAGPV YTGPGASPT QATGEPFAA 
      DSPYFPFSA RPGSRLSPA KPKAGLRPT KEEPPQGNS 
      IDNQWYGSD EFNDSPRYT SPNPTTGYY LGADATPGE 
      WHPSYTTGA YQTYEAYGL IPETEGQGL PPMSSLRPN 
      GTAVTTMSG TNSSAFGPA NHTDGVNKA LGTIYPIVE 
      QASASSYSS APSTPVSSP PPLAPSSWL ANHNTPHSP 
      HYTQTVNVN NSAAPQGLH MPPAPEAQR LDDAIGFLR 
      DHAEVNGTR MEERLDDAI NVLRNHAEP QIIGQLPPP 
      HQMTTLGYT AAASEPHIP DTVKVERAT YNTKKRKEP 
      PDSDTKPSS SVETIVQPN STSGPPPKT TKRSRRYNG 
      DEDEIDDPS NKAVREKER RQANNVRER CSSADETEE 
      GELDPDIKA QREKERRQA NNARERIRI RDINEALKE 
      LGRMCMAHL KTDKPQTKL GILNMAVEV IMTLEQQVR 
      ERNLNPKAA CLKRREEEK AEDGPKLGP GPHHMLTAQ 
      HYPTLPGAP ANLPHNPGQ PQ
    • >TC009743 RC
      MAYAISPAG PVYTGPGAS PTQATGEPF AADSPYFPF 
      SARPGSRLS PAKPKAGLR PTKEEPPQG NSIDNQWYG 
      SDEFNDSPR YTSPNPTTG YYLGADATP GEWHPSYTT 
      GAYQTYEAY GLIPETEGQ GLPPMSSLR PNGTAVTTM 
      SGTNSSAFG PANHTDGVN KALGTIYPI VEQASASSY 
      SSAPSTPVS SPPPLAPSS WLANHNTPH SPHYTQTVN 
      VNNSAAPQG LHMPPAPEA QRLDDAIGF LRDHAEVNG 
      TRMEERLDD AINVLRNHA EPQIIGQLP PPHQMTTLG 
      YTAAASEPH IPDTVKVER ATYNTKKRK EPPDSDTKP 
      SSSVETIVQ PNSTSGPPP KTTKRSRRY NGDEDEIDD 
      PSNKAVREK ERRQANNVR ERCSSADET EEGELDPDI 
      KAQREKERR QANNARERI RIRDINEAL KELGRMCMA 
      HLKTDKPQT KLGILNMAV EVIMTLEQQ VRERNLNPK 
      AACLKRREE EKAEDGPKL GPGPHHMLT AQHYPTLPG 
      APANLPHNP GQPQ
    CDS sequence
    • >TC009743 RA
      atggcctac gccataagt ccggcgggt ccggtttat 
      acgggtccc ggagccagt ccgacacaa gccacagga 
      gaaccgttc gcagcagat agcccttat tttccattc 
      agtgctagg ccgggaagt agactatcg cctgcgaaa 
      cctaaagcc ggccttagg ccgaccaaa gaagaaccg 
      ccacaggga aattcgatc gacaaccag tggtatggt 
      tcggatgaa ttcaacgac tcgccccgc tacacctcg 
      cccaaccct acgacgggt tactatctg ggcgcggat 
      gcaaccccc ggcgaatgg cacccttcg tacaccaca 
      ggcgcgtac caaacgtac gaagcgtac ggtctcatc 
      ccagaaaca gaaggtcaa ggtctgccc ccgatgtct 
      tcgttaagg ccgaacggt accgccgtg accaccatg 
      tccggtaca aacagttcg gccttcggt ccggcaaat 
      cacaccgac ggtgtgaac aaagcttta ggaacaatt 
      tatccaata gtggaacaa gctagcgct agtagttat 
      agttcggcc ccgtcgacg ccggttagt tcgccgcct 
      ccattagcg ccatctagc tggttagcc aatcataac 
      actccacat tcgccccat tatacacaa acggttaat 
      gtgaataat agtgctgca cctcagggt ttacatatg 
      cctcccgcc cccgaagcg caacgctta gatgatgca 
      attgggttt ttaagagac cacgcggaa gtaaatgga 
      acacgtatg gaggaaaga cttgacgac gcaattaac 
      gtattgcgt aatcacgcg gaaccacag atcataggg 
      cagcttcca ccacctcac cagatgacg acactgggc 
      tacacggcg gcagcctcg gaaccacac ataccagac 
      acagttaaa gtagaacgg gcgacatac aatacaaaa 
      aagcgcaaa gagcctcca gatagcgac accaaacca 
      tccagttca gtagaaacg atagtacaa ccaaattca 
      accagtggt cctccacca aaaacgacg aagaggtcg 
      cggagatat aatggtgac gaagatgaa attgatgat 
      ccaagcaac aaggccgta cgcgagaaa gagcgtagg 
      caggccaac aatgtccgt gaacggata cgtattcgt 
      gatattaac gaagcttta aaagaactg ggaagaatg 
      tgcatggcg catctaaag acggacaaa ccacaaaca 
      aagcttggt atcctaaat atggctgtc gaagtcatt 
      atgacttta gaacagcaa gttagagag cgaaacttg 
      aatcctaag gcggcatgt ctgaagcgg cgagaggaa 
      gaaaaggca gaagatggc cctaaatta gggccaggg 
      ccgcatcat atgcttact gcccaacat taccccacc 
      ttaccgggt gctcctgcc aacttgcct cacaatcca 
      ggccagccc caatag
    • >TC009743 RB
      atggctgcc agcgatgaa gagcctatg catcattta 
      tatgaggtt tttacaaat tgttttaat aaaatcgca 
      aacaaggca ccagagaaa cccggtttt cagtcgcca 
      tatggtgga attgacaac ggaatggcc tacgccata 
      agtccggcg ggtccggtt tatacgggt cccggagcc 
      agtccgaca caagccaca ggagaaccg ttcgcagca 
      gatagccct tattttcca ttcagtgct aggccggga 
      agtagacta tcgcctgcg aaacctaaa gccggcctt 
      aggccgacc aaagaagaa ccgccacag ggaaattcg 
      atcgacaac cagtggtat ggttcggat gaattcaac 
      gactcgccc cgctacacc tcgcccaac cctacgacg 
      ggttactat ctgggcgcg gatgcaacc cccggcgaa 
      tggcaccct tcgtacacc acaggcgcg taccaaacg 
      tacgaagcg tacggtctc atcccagaa acagaaggt 
      caaggtctg cccccgatg tcttcgtta aggccgaac 
      ggtaccgcc gtgaccacc atgtccggt acaaacagt 
      tcggccttc ggtccggca aatcacacc gacggtgtg 
      aacaaagct ttaggaaca atttatcca atagtggaa 
      caagctagc gctagtagt tatagttcg gccccgtcg 
      acgccggtt agttcgccg cctccatta gcgccatct 
      agctggtta gccaatcat aacactcca cattcgccc 
      cattataca caaacggtt aatgtgaat aatagtgct 
      gcacctcag ggtttacat atgcctccc gcccccgaa 
      gcgcaacgc ttagatgat gcaattggg tttttaaga 
      gaccacgcg gaagtaaat ggaacacgt atggaggaa 
      agacttgac gacgcaatt aacgtattg cgtaatcac 
      gcggaacca cagatcata gggcagctt ccaccacct 
      caccagatg acgacactg ggctacacg gcggcagcc 
      tcggaacca cacatacca gacacagtt aaagtagaa 
      cgggcgaca tacaataca aaaaagcgc aaagagcct 
      ccagatagc gacaccaaa ccatccagt tcagtagaa 
      acgatagta caaccaaat tcaaccagt ggtcctcca 
      ccaaaaacg acgaagagg tcgcggaga tataatggt 
      gacgaagat gaaattgat gatccaagc aacaaggcc 
      gtacgcgag aaagagcgt aggcaggcc aacaatgtc 
      cgtgaacgt tgttccagt gcggacgaa acggaagag 
      ggtgaacta gatcccgat attaaagcg caaagggag 
      aaagagagg aggcaagcc aataacgct agagaaagg 
      atacgtatt cgtgatatt aacgaagct ttaaaagaa 
      ctgggaaga atgtgcatg gcgcatcta aagacggac 
      aaaccacaa acaaagctt ggtatccta aatatggct 
      gtcgaagtc attatgact ttagaacag caagttaga 
      gagcgaaac ttgaatcct aaggcggca tgtctgaag 
      cggcgagag gaagaaaag gcagaagat ggccctaaa 
      ttagggcca gggccgcat catatgctt actgcccaa 
      cattacccc accttaccg ggtgctcct gccaacttg 
      cctcacaat ccaggccag ccccaatag
    • >TC009743 RC
      atggcctac gccataagt ccggcgggt ccggtttat 
      acgggtccc ggagccagt ccgacacaa gccacagga 
      gaaccgttc gcagcagat agcccttat tttccattc 
      agtgctagg ccgggaagt agactatcg cctgcgaaa 
      cctaaagcc ggccttagg ccgaccaaa gaagaaccg 
      ccacaggga aattcgatc gacaaccag tggtatggt 
      tcggatgaa ttcaacgac tcgccccgc tacacctcg 
      cccaaccct acgacgggt tactatctg ggcgcggat 
      gcaaccccc ggcgaatgg cacccttcg tacaccaca 
      ggcgcgtac caaacgtac gaagcgtac ggtctcatc 
      ccagaaaca gaaggtcaa ggtctgccc ccgatgtct 
      tcgttaagg ccgaacggt accgccgtg accaccatg 
      tccggtaca aacagttcg gccttcggt ccggcaaat 
      cacaccgac ggtgtgaac aaagcttta ggaacaatt 
      tatccaata gtggaacaa gctagcgct agtagttat 
      agttcggcc ccgtcgacg ccggttagt tcgccgcct 
      ccattagcg ccatctagc tggttagcc aatcataac 
      actccacat tcgccccat tatacacaa acggttaat 
      gtgaataat agtgctgca cctcagggt ttacatatg 
      cctcccgcc cccgaagcg caacgctta gatgatgca 
      attgggttt ttaagagac cacgcggaa gtaaatgga 
      acacgtatg gaggaaaga cttgacgac gcaattaac 
      gtattgcgt aatcacgcg gaaccacag atcataggg 
      cagcttcca ccacctcac cagatgacg acactgggc 
      tacacggcg gcagcctcg gaaccacac ataccagac 
      acagttaaa gtagaacgg gcgacatac aatacaaaa 
      aagcgcaaa gagcctcca gatagcgac accaaacca 
      tccagttca gtagaaacg atagtacaa ccaaattca 
      accagtggt cctccacca aaaacgacg aagaggtcg 
      cggagatat aatggtgac gaagatgaa attgatgat 
      ccaagcaac aaggccgta cgcgagaaa gagcgtagg 
      caggccaac aatgtccgt gaacgttgt tccagtgcg 
      gacgaaacg gaagagggt gaactagat cccgatatt 
      aaagcgcaa agggagaaa gagaggagg caagccaat 
      aacgctaga gaaaggata cgtattcgt gatattaac 
      gaagcttta aaagaactg ggaagaatg tgcatggcg 
      catctaaag acggacaaa ccacaaaca aagcttggt 
      atcctaaat atggctgtc gaagtcatt atgacttta 
      gaacagcaa gttagagag cgaaacttg aatcctaag 
      gcggcatgt ctgaagcgg cgagaggaa gaaaaggca 
      gaagatggc cctaaatta gggccaggg ccgcatcat 
      atgcttact gcccaacat taccccacc ttaccgggt 
      gctcctgcc aacttgcct cacaatcca ggccagccc 
      caatag
    mRNA sequence
    • >TC009743 RA
      ggtgtcgtc agcgtcgtc agttgtcag tagtaagtc 
      aaaatggct gccagcgat gaagagcct atgcatcat 
      ttatatgag gtttttaca aattgtttt aataaaatc 
      gcaaacaag gcaccagag aaacccggt tttcagtcg 
      ccatatggt ggaattgac aacggaatg gcctacgcc 
      ataagtccg gcgggtccg gtttatacg ggtcccgga 
      gccagtccg acacaagcc acaggagaa ccgttcgca 
      gcagatagc ccttatttt ccattcagt gctaggccg 
      ggaagtaga ctatcgcct gcgaaacct aaagccggc 
      cttaggccg accaaagaa gaaccgcca cagggaaat 
      tcgatcgac aaccagtgg tatggttcg gatgaattc 
      aacgactcg ccccgctac acctcgccc aaccctacg 
      acgggttac tatctgggc gcggatgca acccccggc 
      gaatggcac ccttcgtac accacaggc gcgtaccaa 
      acgtacgaa gcgtacggt ctcatccca gaaacagaa 
      ggtcaaggt ctgcccccg atgtcttcg ttaaggccg 
      aacggtacc gccgtgacc accatgtcc ggtacaaac 
      agttcggcc ttcggtccg gcaaatcac accgacggt 
      gtgaacaaa gctttagga acaatttat ccaatagtg 
      gaacaagct agcgctagt agttatagt tcggccccg 
      tcgacgccg gttagttcg ccgcctcca ttagcgcca 
      tctagctgg ttagccaat cataacact ccacattcg 
      ccccattat acacaaacg gttaatgtg aataatagt 
      gctgcacct cagggttta catatgcct cccgccccc 
      gaagcgcaa cgcttagat gatgcaatt gggttttta 
      agagaccac gcggaagta aatggaaca cgtatggag 
      gaaagactt gacgacgca attaacgta ttgcgtaat 
      cacgcggaa ccacagatc atagggcag cttccacca 
      cctcaccag atgacgaca ctgggctac acggcggca 
      gcctcggaa ccacacata ccagacaca gttaaagta 
      gaacgggcg acatacaat acaaaaaag cgcaaagag 
      cctccagat agcgacacc aaaccatcc agttcagta 
      gaaacgata gtacaacca aattcaacc agtggtcct 
      ccaccaaaa acgacgaag aggtcgcgg agatataat 
      ggtgacgaa gatgaaatt gatgatcca agcaacaag 
      gccgtacgc gagaaagag cgtaggcag gccaacaat 
      gtccgtgaa cggatacgt attcgtgat attaacgaa 
      gctttaaaa gaactggga agaatgtgc atggcgcat 
      ctaaagacg gacaaacca caaacaaag cttggtatc 
      ctaaatatg gctgtcgaa gtcattatg actttagaa 
      cagcaagtt agagagcga aacttgaat cctaaggcg 
      gcatgtctg aagcggcga gaggaagaa aaggcagaa 
      gatggccct aaattaggg ccagggccg catcatatg 
      cttactgcc caacattac cccacctta ccgggtgct 
      cctgccaac ttgcctcac aatccaggc cagccccaa 
      tagcactgc taaaacggc aggtctgcc acctgtaca 
      taacttttc gtacaaacg gctctcatc atttgttca 
      tcaatgatc atgcaaaca aactatatt atatttttt 
      taagaatgt tctgtagtg atacaataa cgtatttta 
      ttgttttat aaattactt ttcacttta ttgagatgg 
      aaaaacgag aggtttgac gacattttt ttaccgagc 
      gtatcgacg cacgaccgg agcagtttg tttcttttt 
      ttttaatac atgtcgaac aattttgtg tgtgccaaa 
      tataagaaa cacaatact cttaccgtg aaacaaata 
      cgtacgttt ttgatctct ctttgccat gatttttgt 
      gacatattt tcgtagttc gtagcaaaa ttgtgccat 
      tcgttttga atttttttc gccttttaa tgataatgc 
      gagaaaaat tcgatcggt actccgatc gtgcgttga 
      ccgaaattg atacatgcg ccagtattt tgaaaatta 
      tgacttata gaattttta tgggaagtc gtcttctca 
      tcacaaact gtaaataag tgttcatta gtgtgaccg 
      gtgacgttt ctccagtat tttgatatg caatataaa 
      atttaactt acgtattac gaaaagggg agaggtttc 
      cggtgctct cttaaatat atgccattg aaaaatgaa 
      cataactta agatattac ttatttaat attaaacag 
      tctgtttat tcactgaac tattttttt atcttatat 
      attttatta tttgagcgg tagattttt ttaattatt 
      ctactaaac ttagtgcaa cttttaaat ttagtttgg 
      tgttcaaac tataaccag atatatcga taaattttt 
      gttcattat ttttaattt taagtttta tgtaatatt 
      taactataa tttttatta ggtatattt tactttaag 
      gtaaagatg taattttgc attgtttct cttttagct 
      ttttagtga atctttatt tatatttgt ttttattgt 
      taaaaaagc tcagtttga atttcgacg gagttcaac 
      cgacggttt attgttgca atgcaatca ggatttagc 
      gtcattgta aattttttg caataactc gtaatggca 
      tagtatatt tttcattcc accggcagc cattttgta 
      ctattattt attaggcag caaatggaa gacattaat 
      cgcggcagc cgtgcataa acacaatca ttcatcttc 
      tattttggc tcaattttc aatattggt gcaactaat 
      ttttttgtt tttgtatta ttctaaaca ttttgtgaa 
      acgaatctt gatagttaa cattgttgt ctaccatta 
      cacaaattg tttagacgt ttgataatg atatatatt 
      tttttagtt agtggacaa catgttatt cttgagatt 
      tggctctta aacgattag acgatttgt aaagcttac 
      cttgacatg tataaacgt ttaaagcgg atgtgtttt 
      taagtatat aaaaaagta tatatgaat aaaatacag 
      cgtgatgaa aaagtaaac ttaagatat acagttaga 
      atacggtat aaaatgaat atcaatgta atatacaat 
      acgtacata atatact
    • >TC009743 RB
      ggtgtcgtc agcgtcgtc agttgtcag tagtaagtc 
      aaaatggct gccagcgat gaagagcct atgcatcat 
      ttatatgag gtttttaca aattgtttt aataaaatc 
      gcaaacaag gcaccagag aaacccggt tttcagtcg 
      ccatatggt ggaattgac aacggaatg gcctacgcc 
      ataagtccg gcgggtccg gtttatacg ggtcccgga 
      gccagtccg acacaagcc acaggagaa ccgttcgca 
      gcagatagc ccttatttt ccattcagt gctaggccg 
      ggaagtaga ctatcgcct gcgaaacct aaagccggc 
      cttaggccg accaaagaa gaaccgcca cagggaaat 
      tcgatcgac aaccagtgg tatggttcg gatgaattc 
      aacgactcg ccccgctac acctcgccc aaccctacg 
      acgggttac tatctgggc gcggatgca acccccggc 
      gaatggcac ccttcgtac accacaggc gcgtaccaa 
      acgtacgaa gcgtacggt ctcatccca gaaacagaa 
      ggtcaaggt ctgcccccg atgtcttcg ttaaggccg 
      aacggtacc gccgtgacc accatgtcc ggtacaaac 
      agttcggcc ttcggtccg gcaaatcac accgacggt 
      gtgaacaaa gctttagga acaatttat ccaatagtg 
      gaacaagct agcgctagt agttatagt tcggccccg 
      tcgacgccg gttagttcg ccgcctcca ttagcgcca 
      tctagctgg ttagccaat cataacact ccacattcg 
      ccccattat acacaaacg gttaatgtg aataatagt 
      gctgcacct cagggttta catatgcct cccgccccc 
      gaagcgcaa cgcttagat gatgcaatt gggttttta 
      agagaccac gcggaagta aatggaaca cgtatggag 
      gaaagactt gacgacgca attaacgta ttgcgtaat 
      cacgcggaa ccacagatc atagggcag cttccacca 
      cctcaccag atgacgaca ctgggctac acggcggca 
      gcctcggaa ccacacata ccagacaca gttaaagta 
      gaacgggcg acatacaat acaaaaaag cgcaaagag 
      cctccagat agcgacacc aaaccatcc agttcagta 
      gaaacgata gtacaacca aattcaacc agtggtcct 
      ccaccaaaa acgacgaag aggtcgcgg agatataat 
      ggtgacgaa gatgaaatt gatgatcca agcaacaag 
      gccgtacgc gagaaagag cgtaggcag gccaacaat 
      gtccgtgaa cgttgttcc agtgcggac gaaacggaa 
      gagggtgaa ctagatccc gatattaaa gcgcaaagg 
      gagaaagag aggaggcaa gccaataac gctagagaa 
      aggatacgt attcgtgat attaacgaa gctttaaaa 
      gaactggga agaatgtgc atggcgcat ctaaagacg 
      gacaaacca caaacaaag cttggtatc ctaaatatg 
      gctgtcgaa gtcattatg actttagaa cagcaagtt 
      agagagcga aacttgaat cctaaggcg gcatgtctg 
      aagcggcga gaggaagaa aaggcagaa gatggccct 
      aaattaggg ccagggccg catcatatg cttactgcc 
      caacattac cccacctta ccgggtgct cctgccaac 
      ttgcctcac aatccaggc cagccccaa tagcactgc 
      taaaacggc aggtctgcc acctgtaca taacttttc 
      gtacaaacg gctctcatc atttgttca tcaatgatc 
      atgcaaaca aactatatt atatttttt taagaatgt 
      tctgtagtg atacaataa cgtatttta ttgttttat 
      aaattactt ttcacttta ttgagatgg aaaaacgag 
      aggtttgac gacattttt ttaccgagc gtatcgacg 
      cacgaccgg agcagtttg tttcttttt ttttaatac 
      atgtcgaac aattttgtg tgtgccaaa tataagaaa 
      cacaatact cttaccgtg aaacaaata cgtacgttt 
      ttgatctct ctttgccat gatttttgt gacatattt 
      tcgtagttc gtagcaaaa ttgtgccat tcgttttga 
      atttttttc gccttttaa tgataatgc gagaaaaat 
      tcgatcggt actccgatc gtgcgttga ccgaaattg 
      atacatgcg ccagtattt tgaaaatta tgacttata 
      gaattttta tgggaagtc gtcttctca tcacaaact 
      gtaaataag tgttcatta gtgtgaccg gtgacgttt 
      ctccagtat tttgatatg caatataaa atttaactt 
      acgtattac gaaaagggg agaggtttc cggtgctct 
      cttaaatat atgccattg aaaaatgaa cataactta 
      agatattac ttatttaat attaaacag tctgtttat 
      tcactgaac tattttttt atcttatat attttatta 
      tttgagcgg tagattttt ttaattatt ctactaaac 
      ttagtgcaa cttttaaat ttagtttgg tgttcaaac 
      tataaccag atatatcga taaattttt gttcattat 
      ttttaattt taagtttta tgtaatatt taactataa 
      tttttatta ggtatattt tactttaag gtaaagatg 
      taattttgc attgtttct cttttagct ttttagtga 
      atctttatt tatatttgt ttttattgt taaaaaagc 
      tcagtttga atttcgacg gagttcaac cgacggttt 
      attgttgca atgcaatca ggatttagc gtcattgta 
      aattttttg caataactc gtaatggca tagtatatt 
      tttcattcc accggcagc cattttgta ctattattt 
      attaggcag caaatggaa gacattaat cgcggcagc 
      cgtgcataa acacaatca ttcatcttc tattttggc 
      tcaattttc aatattggt gcaactaat ttttttgtt 
      tttgtatta ttctaaaca ttttgtgaa acgaatctt 
      gatagttaa cattgttgt ctaccatta cacaaattg 
      tttagacgt ttgataatg atatatatt tttttagtt 
      agtggacaa catgttatt cttgagatt tggctctta 
      aacgattag acgatttgt aaagcttac cttgacatg 
      tataaacgt ttaaagcgg atgtgtttt taagtatat 
      aaaaaagta tatatgaat aaaatacag cgtgatgaa 
      aaagtaaac ttaagatat acagttaga atacggtat 
      aaaatgaat atcaatgta atatacaat acgtacata 
      atatact
    • >TC009743 RC
      ggtgtcgtc agcgtcgtc agttgtcag tagtaagtc 
      aaaatggct gccagcgat gaagagcct atgcatcat 
      ttatatgag gtttttaca aattgtttt aataaaatc 
      gcaaacaag gcaccagag aaacccggt tttcagtcg 
      ccatatggt ggaattgac aacggaatg gcctacgcc 
      ataagtccg gcgggtccg gtttatacg ggtcccgga 
      gccagtccg acacaagcc acaggagaa ccgttcgca 
      gcagatagc ccttatttt ccattcagt gctaggccg 
      ggaagtaga ctatcgcct gcgaaacct aaagccggc 
      cttaggccg accaaagaa gaaccgcca cagggaaat 
      tcgatcgac aaccagtgg tatggttcg gatgaattc 
      aacgactcg ccccgctac acctcgccc aaccctacg 
      acgggttac tatctgggc gcggatgca acccccggc 
      gaatggcac ccttcgtac accacaggc gcgtaccaa 
      acgtacgaa gcgtacggt ctcatccca gaaacagaa 
      ggtcaaggt ctgcccccg atgtcttcg ttaaggccg 
      aacggtacc gccgtgacc accatgtcc ggtacaaac 
      agttcggcc ttcggtccg gcaaatcac accgacggt 
      gtgaacaaa gctttagga acaatttat ccaatagtg 
      gaacaagct agcgctagt agttatagt tcggccccg 
      tcgacgccg gttagttcg ccgcctcca ttagcgcca 
      tctagctgg ttagccaat cataacact ccacattcg 
      ccccattat acacaaacg gttaatgtg aataatagt 
      gctgcacct cagggttta catatgcct cccgccccc 
      gaagcgcaa cgcttagat gatgcaatt gggttttta 
      agagaccac gcggaagta aatggaaca cgtatggag 
      gaaagactt gacgacgca attaacgta ttgcgtaat 
      cacgcggaa ccacagatc atagggcag cttccacca 
      cctcaccag atgacgaca ctgggctac acggcggca 
      gcctcggaa ccacacata ccagacaca gttaaagta 
      gaacgggcg acatacaat acaaaaaag cgcaaagag 
      cctccagat agcgacacc aaaccatcc agttcagta 
      gaaacgata gtacaacca aattcaacc agtggtcct 
      ccaccaaaa acgacgaag aggtcgcgg agatataat 
      ggtgacgaa gatgaaatt gatgatcca agcaacaag 
      gccgtacgc gagaaagag cgtaggcag gccaacaat 
      gtccgtgaa cgttgttcc agtgcggac gaaacggaa 
      gagggtgaa ctagatccc gatattaaa gcgcaaagg 
      gagaaagag aggaggcaa gccaataac gctagagaa 
      aggatacgt attcgtgat attaacgaa gctttaaaa 
      gaactggga agaatgtgc atggcgcat ctaaagacg 
      gacaaacca caaacaaag cttggtatc ctaaatatg 
      gctgtcgaa gtcattatg actttagaa cagcaagtt 
      agagagcga aacttgaat cctaaggcg gcatgtctg 
      aagcggcga gaggaagaa aaggcagaa gatggccct 
      aaattaggg ccagggccg catcatatg cttactgcc 
      caacattac cccacctta ccgggtgct cctgccaac 
      ttgcctcac aatccaggc cagccccaa tagcactgc 
      taaaacggc aggtctgcc acctgtaca taacttttc 
      gtacaaacg gctctcatc atttgttca tcaatgatc 
      atgcaaaca aactatatt atatttttt taagaatgt 
      tctgtagtg atacaataa cgtatttta ttgttttat 
      aaattactt ttcacttta ttgagatgg aaaaacgag 
      aggtttgac gacattttt ttaccgagc gtatcgacg 
      cacgaccgg agcagtttg tttcttttt ttttaatac 
      atgtcgaac aattttgtg tgtgccaaa tataagaaa 
      cacaatact cttaccgtg aaacaaata cgtacgttt 
      ttgatctct ctttgccat gatttttgt gacatattt 
      tcgtagttc gtagcaaaa ttgtgccat tcgttttga 
      atttttttc gccttttaa tgataatgc gagaaaaat 
      tcgatcggt actccgatc gtgcgttga ccgaaattg 
      atacatgcg ccagtattt tgaaaatta tgacttata 
      gaattttta tgggaagtc gtcttctca tcacaaact 
      gtaaataag tgttcatta gtgtgaccg gtgacgttt 
      ctccagtat tttgatatg caatataaa atttaactt 
      acgtattac gaaaagggg agaggtttc cggtgctct 
      cttaaatat atgccattg aaaaatgaa cataactta 
      agatattac ttatttaat attaaacag tctgtttat 
      tcactgaac tattttttt atcttatat attttatta 
      tttgagcgg tagattttt ttaattatt ctactaaac 
      ttagtgcaa cttttaaat ttagtttgg tgttcaaac 
      tataaccag atatatcga taaattttt gttcattat 
      ttttaattt taagtttta tgtaatatt taactataa 
      tttttatta ggtatattt tactttaag gtaaagatg 
      taattttgc attgtttct cttttagct ttttagtga 
      atctttatt tatatttgt ttttattgt taaaaaagc 
      tcagtttga atttcgacg gagttcaac cgacggttt 
      attgttgca atgcaatca ggatttagc gtcattgta 
      aattttttg caataactc gtaatggca tagtatatt 
      tttcattcc accggcagc cattttgta ctattattt 
      attaggcag caaatggaa gacattaat cgcggcagc 
      cgtgcataa acacaatca ttcatcttc tattttggc 
      tcaattttc aatattggt gcaactaat ttttttgtt 
      tttgtatta ttctaaaca ttttgtgaa acgaatctt 
      gatagttaa cattgttgt ctaccatta cacaaattg 
      tttagacgt ttgataatg atatatatt tttttagtt 
      agtggacaa catgttatt cttgagatt tggctctta 
      aacgattag acgatttgt aaagcttac cttgacatg 
      tataaacgt ttaaagcgg atgtgtttt taagtatat 
      aaaaaagta tatatgaat aaaatacag cgtgatgaa 
      aaagtaaac ttaagatat acagttaga atacggtat 
      aaaatgaat atcaatgta atatacaat acgtacata 
      atatact
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