| General information | |||
| TC009743 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 7171381..7187105 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009743 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02849 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC009743 RA
MAYAISPAG PVYTGPGAS PTQATGEPF AADSPYFPF SARPGSRLS PAKPKAGLR PTKEEPPQG NSIDNQWYG SDEFNDSPR YTSPNPTTG YYLGADATP GEWHPSYTT GAYQTYEAY GLIPETEGQ GLPPMSSLR PNGTAVTTM SGTNSSAFG PANHTDGVN KALGTIYPI VEQASASSY SSAPSTPVS SPPPLAPSS WLANHNTPH SPHYTQTVN VNNSAAPQG LHMPPAPEA QRLDDAIGF LRDHAEVNG TRMEERLDD AINVLRNHA EPQIIGQLP PPHQMTTLG YTAAASEPH IPDTVKVER ATYNTKKRK EPPDSDTKP SSSVETIVQ PNSTSGPPP KTTKRSRRY NGDEDEIDD PSNKAVREK ERRQANNVR ERIRIRDIN EALKELGRM CMAHLKTDK PQTKLGILN MAVEVIMTL EQQVRERNL NPKAACLKR REEEKAEDG PKLGPGPHH MLTAQHYPT LPGAPANLP HNPGQPQ
- >TC009743 RB
MAASDEEPM HHLYEVFTN CFNKIANKA PEKPGFQSP YGGIDNGMA YAISPAGPV YTGPGASPT QATGEPFAA DSPYFPFSA RPGSRLSPA KPKAGLRPT KEEPPQGNS IDNQWYGSD EFNDSPRYT SPNPTTGYY LGADATPGE WHPSYTTGA YQTYEAYGL IPETEGQGL PPMSSLRPN GTAVTTMSG TNSSAFGPA NHTDGVNKA LGTIYPIVE QASASSYSS APSTPVSSP PPLAPSSWL ANHNTPHSP HYTQTVNVN NSAAPQGLH MPPAPEAQR LDDAIGFLR DHAEVNGTR MEERLDDAI NVLRNHAEP QIIGQLPPP HQMTTLGYT AAASEPHIP DTVKVERAT YNTKKRKEP PDSDTKPSS SVETIVQPN STSGPPPKT TKRSRRYNG DEDEIDDPS NKAVREKER RQANNVRER CSSADETEE GELDPDIKA QREKERRQA NNARERIRI RDINEALKE LGRMCMAHL KTDKPQTKL GILNMAVEV IMTLEQQVR ERNLNPKAA CLKRREEEK AEDGPKLGP GPHHMLTAQ HYPTLPGAP ANLPHNPGQ PQ
- >TC009743 RC
MAYAISPAG PVYTGPGAS PTQATGEPF AADSPYFPF SARPGSRLS PAKPKAGLR PTKEEPPQG NSIDNQWYG SDEFNDSPR YTSPNPTTG YYLGADATP GEWHPSYTT GAYQTYEAY GLIPETEGQ GLPPMSSLR PNGTAVTTM SGTNSSAFG PANHTDGVN KALGTIYPI VEQASASSY SSAPSTPVS SPPPLAPSS WLANHNTPH SPHYTQTVN VNNSAAPQG LHMPPAPEA QRLDDAIGF LRDHAEVNG TRMEERLDD AINVLRNHA EPQIIGQLP PPHQMTTLG YTAAASEPH IPDTVKVER ATYNTKKRK EPPDSDTKP SSSVETIVQ PNSTSGPPP KTTKRSRRY NGDEDEIDD PSNKAVREK ERRQANNVR ERCSSADET EEGELDPDI KAQREKERR QANNARERI RIRDINEAL KELGRMCMA HLKTDKPQT KLGILNMAV EVIMTLEQQ VRERNLNPK AACLKRREE EKAEDGPKL GPGPHHMLT AQHYPTLPG APANLPHNP GQPQ
- >TC009743 RA
atggcctac gccataagt ccggcgggt ccggtttat acgggtccc ggagccagt ccgacacaa gccacagga gaaccgttc gcagcagat agcccttat tttccattc agtgctagg ccgggaagt agactatcg cctgcgaaa cctaaagcc ggccttagg ccgaccaaa gaagaaccg ccacaggga aattcgatc gacaaccag tggtatggt tcggatgaa ttcaacgac tcgccccgc tacacctcg cccaaccct acgacgggt tactatctg ggcgcggat gcaaccccc ggcgaatgg cacccttcg tacaccaca ggcgcgtac caaacgtac gaagcgtac ggtctcatc ccagaaaca gaaggtcaa ggtctgccc ccgatgtct tcgttaagg ccgaacggt accgccgtg accaccatg tccggtaca aacagttcg gccttcggt ccggcaaat cacaccgac ggtgtgaac aaagcttta ggaacaatt tatccaata gtggaacaa gctagcgct agtagttat agttcggcc ccgtcgacg ccggttagt tcgccgcct ccattagcg ccatctagc tggttagcc aatcataac actccacat tcgccccat tatacacaa acggttaat gtgaataat agtgctgca cctcagggt ttacatatg cctcccgcc cccgaagcg caacgctta gatgatgca attgggttt ttaagagac cacgcggaa gtaaatgga acacgtatg gaggaaaga cttgacgac gcaattaac gtattgcgt aatcacgcg gaaccacag atcataggg cagcttcca ccacctcac cagatgacg acactgggc tacacggcg gcagcctcg gaaccacac ataccagac acagttaaa gtagaacgg gcgacatac aatacaaaa aagcgcaaa gagcctcca gatagcgac accaaacca tccagttca gtagaaacg atagtacaa ccaaattca accagtggt cctccacca aaaacgacg aagaggtcg cggagatat aatggtgac gaagatgaa attgatgat ccaagcaac aaggccgta cgcgagaaa gagcgtagg caggccaac aatgtccgt gaacggata cgtattcgt gatattaac gaagcttta aaagaactg ggaagaatg tgcatggcg catctaaag acggacaaa ccacaaaca aagcttggt atcctaaat atggctgtc gaagtcatt atgacttta gaacagcaa gttagagag cgaaacttg aatcctaag gcggcatgt ctgaagcgg cgagaggaa gaaaaggca gaagatggc cctaaatta gggccaggg ccgcatcat atgcttact gcccaacat taccccacc ttaccgggt gctcctgcc aacttgcct cacaatcca ggccagccc caatag
- >TC009743 RB
atggctgcc agcgatgaa gagcctatg catcattta tatgaggtt tttacaaat tgttttaat aaaatcgca aacaaggca ccagagaaa cccggtttt cagtcgcca tatggtgga attgacaac ggaatggcc tacgccata agtccggcg ggtccggtt tatacgggt cccggagcc agtccgaca caagccaca ggagaaccg ttcgcagca gatagccct tattttcca ttcagtgct aggccggga agtagacta tcgcctgcg aaacctaaa gccggcctt aggccgacc aaagaagaa ccgccacag ggaaattcg atcgacaac cagtggtat ggttcggat gaattcaac gactcgccc cgctacacc tcgcccaac cctacgacg ggttactat ctgggcgcg gatgcaacc cccggcgaa tggcaccct tcgtacacc acaggcgcg taccaaacg tacgaagcg tacggtctc atcccagaa acagaaggt caaggtctg cccccgatg tcttcgtta aggccgaac ggtaccgcc gtgaccacc atgtccggt acaaacagt tcggccttc ggtccggca aatcacacc gacggtgtg aacaaagct ttaggaaca atttatcca atagtggaa caagctagc gctagtagt tatagttcg gccccgtcg acgccggtt agttcgccg cctccatta gcgccatct agctggtta gccaatcat aacactcca cattcgccc cattataca caaacggtt aatgtgaat aatagtgct gcacctcag ggtttacat atgcctccc gcccccgaa gcgcaacgc ttagatgat gcaattggg tttttaaga gaccacgcg gaagtaaat ggaacacgt atggaggaa agacttgac gacgcaatt aacgtattg cgtaatcac gcggaacca cagatcata gggcagctt ccaccacct caccagatg acgacactg ggctacacg gcggcagcc tcggaacca cacatacca gacacagtt aaagtagaa cgggcgaca tacaataca aaaaagcgc aaagagcct ccagatagc gacaccaaa ccatccagt tcagtagaa acgatagta caaccaaat tcaaccagt ggtcctcca ccaaaaacg acgaagagg tcgcggaga tataatggt gacgaagat gaaattgat gatccaagc aacaaggcc gtacgcgag aaagagcgt aggcaggcc aacaatgtc cgtgaacgt tgttccagt gcggacgaa acggaagag ggtgaacta gatcccgat attaaagcg caaagggag aaagagagg aggcaagcc aataacgct agagaaagg atacgtatt cgtgatatt aacgaagct ttaaaagaa ctgggaaga atgtgcatg gcgcatcta aagacggac aaaccacaa acaaagctt ggtatccta aatatggct gtcgaagtc attatgact ttagaacag caagttaga gagcgaaac ttgaatcct aaggcggca tgtctgaag cggcgagag gaagaaaag gcagaagat ggccctaaa ttagggcca gggccgcat catatgctt actgcccaa cattacccc accttaccg ggtgctcct gccaacttg cctcacaat ccaggccag ccccaatag
- >TC009743 RC
atggcctac gccataagt ccggcgggt ccggtttat acgggtccc ggagccagt ccgacacaa gccacagga gaaccgttc gcagcagat agcccttat tttccattc agtgctagg ccgggaagt agactatcg cctgcgaaa cctaaagcc ggccttagg ccgaccaaa gaagaaccg ccacaggga aattcgatc gacaaccag tggtatggt tcggatgaa ttcaacgac tcgccccgc tacacctcg cccaaccct acgacgggt tactatctg ggcgcggat gcaaccccc ggcgaatgg cacccttcg tacaccaca ggcgcgtac caaacgtac gaagcgtac ggtctcatc ccagaaaca gaaggtcaa ggtctgccc ccgatgtct tcgttaagg ccgaacggt accgccgtg accaccatg tccggtaca aacagttcg gccttcggt ccggcaaat cacaccgac ggtgtgaac aaagcttta ggaacaatt tatccaata gtggaacaa gctagcgct agtagttat agttcggcc ccgtcgacg ccggttagt tcgccgcct ccattagcg ccatctagc tggttagcc aatcataac actccacat tcgccccat tatacacaa acggttaat gtgaataat agtgctgca cctcagggt ttacatatg cctcccgcc cccgaagcg caacgctta gatgatgca attgggttt ttaagagac cacgcggaa gtaaatgga acacgtatg gaggaaaga cttgacgac gcaattaac gtattgcgt aatcacgcg gaaccacag atcataggg cagcttcca ccacctcac cagatgacg acactgggc tacacggcg gcagcctcg gaaccacac ataccagac acagttaaa gtagaacgg gcgacatac aatacaaaa aagcgcaaa gagcctcca gatagcgac accaaacca tccagttca gtagaaacg atagtacaa ccaaattca accagtggt cctccacca aaaacgacg aagaggtcg cggagatat aatggtgac gaagatgaa attgatgat ccaagcaac aaggccgta cgcgagaaa gagcgtagg caggccaac aatgtccgt gaacgttgt tccagtgcg gacgaaacg gaagagggt gaactagat cccgatatt aaagcgcaa agggagaaa gagaggagg caagccaat aacgctaga gaaaggata cgtattcgt gatattaac gaagcttta aaagaactg ggaagaatg tgcatggcg catctaaag acggacaaa ccacaaaca aagcttggt atcctaaat atggctgtc gaagtcatt atgacttta gaacagcaa gttagagag cgaaacttg aatcctaag gcggcatgt ctgaagcgg cgagaggaa gaaaaggca gaagatggc cctaaatta gggccaggg ccgcatcat atgcttact gcccaacat taccccacc ttaccgggt gctcctgcc aacttgcct cacaatcca ggccagccc caatag
- >TC009743 RA
ggtgtcgtc agcgtcgtc agttgtcag tagtaagtc aaaatggct gccagcgat gaagagcct atgcatcat ttatatgag gtttttaca aattgtttt aataaaatc gcaaacaag gcaccagag aaacccggt tttcagtcg ccatatggt ggaattgac aacggaatg gcctacgcc ataagtccg gcgggtccg gtttatacg ggtcccgga gccagtccg acacaagcc acaggagaa ccgttcgca gcagatagc ccttatttt ccattcagt gctaggccg ggaagtaga ctatcgcct gcgaaacct aaagccggc cttaggccg accaaagaa gaaccgcca cagggaaat tcgatcgac aaccagtgg tatggttcg gatgaattc aacgactcg ccccgctac acctcgccc aaccctacg acgggttac tatctgggc gcggatgca acccccggc gaatggcac ccttcgtac accacaggc gcgtaccaa acgtacgaa gcgtacggt ctcatccca gaaacagaa ggtcaaggt ctgcccccg atgtcttcg ttaaggccg aacggtacc gccgtgacc accatgtcc ggtacaaac agttcggcc ttcggtccg gcaaatcac accgacggt gtgaacaaa gctttagga acaatttat ccaatagtg gaacaagct agcgctagt agttatagt tcggccccg tcgacgccg gttagttcg ccgcctcca ttagcgcca tctagctgg ttagccaat cataacact ccacattcg ccccattat acacaaacg gttaatgtg aataatagt gctgcacct cagggttta catatgcct cccgccccc gaagcgcaa cgcttagat gatgcaatt gggttttta agagaccac gcggaagta aatggaaca cgtatggag gaaagactt gacgacgca attaacgta ttgcgtaat cacgcggaa ccacagatc atagggcag cttccacca cctcaccag atgacgaca ctgggctac acggcggca gcctcggaa ccacacata ccagacaca gttaaagta gaacgggcg acatacaat acaaaaaag cgcaaagag cctccagat agcgacacc aaaccatcc agttcagta gaaacgata gtacaacca aattcaacc agtggtcct ccaccaaaa acgacgaag aggtcgcgg agatataat ggtgacgaa gatgaaatt gatgatcca agcaacaag gccgtacgc gagaaagag cgtaggcag gccaacaat gtccgtgaa cggatacgt attcgtgat attaacgaa gctttaaaa gaactggga agaatgtgc atggcgcat ctaaagacg gacaaacca caaacaaag cttggtatc ctaaatatg gctgtcgaa gtcattatg actttagaa cagcaagtt agagagcga aacttgaat cctaaggcg gcatgtctg aagcggcga gaggaagaa aaggcagaa gatggccct aaattaggg ccagggccg catcatatg cttactgcc caacattac cccacctta ccgggtgct cctgccaac ttgcctcac aatccaggc cagccccaa tagcactgc taaaacggc aggtctgcc acctgtaca taacttttc gtacaaacg gctctcatc atttgttca tcaatgatc atgcaaaca aactatatt atatttttt taagaatgt tctgtagtg atacaataa cgtatttta ttgttttat aaattactt ttcacttta ttgagatgg aaaaacgag aggtttgac gacattttt ttaccgagc gtatcgacg cacgaccgg agcagtttg tttcttttt ttttaatac atgtcgaac aattttgtg tgtgccaaa tataagaaa cacaatact cttaccgtg aaacaaata cgtacgttt ttgatctct ctttgccat gatttttgt gacatattt tcgtagttc gtagcaaaa ttgtgccat tcgttttga atttttttc gccttttaa tgataatgc gagaaaaat tcgatcggt actccgatc gtgcgttga ccgaaattg atacatgcg ccagtattt tgaaaatta tgacttata gaattttta tgggaagtc gtcttctca tcacaaact gtaaataag tgttcatta gtgtgaccg gtgacgttt ctccagtat tttgatatg caatataaa atttaactt acgtattac gaaaagggg agaggtttc cggtgctct cttaaatat atgccattg aaaaatgaa cataactta agatattac ttatttaat attaaacag tctgtttat tcactgaac tattttttt atcttatat attttatta tttgagcgg tagattttt ttaattatt ctactaaac ttagtgcaa cttttaaat ttagtttgg tgttcaaac tataaccag atatatcga taaattttt gttcattat ttttaattt taagtttta tgtaatatt taactataa tttttatta ggtatattt tactttaag gtaaagatg taattttgc attgtttct cttttagct ttttagtga atctttatt tatatttgt ttttattgt taaaaaagc tcagtttga atttcgacg gagttcaac cgacggttt attgttgca atgcaatca ggatttagc gtcattgta aattttttg caataactc gtaatggca tagtatatt tttcattcc accggcagc cattttgta ctattattt attaggcag caaatggaa gacattaat cgcggcagc cgtgcataa acacaatca ttcatcttc tattttggc tcaattttc aatattggt gcaactaat ttttttgtt tttgtatta ttctaaaca ttttgtgaa acgaatctt gatagttaa cattgttgt ctaccatta cacaaattg tttagacgt ttgataatg atatatatt tttttagtt agtggacaa catgttatt cttgagatt tggctctta aacgattag acgatttgt aaagcttac cttgacatg tataaacgt ttaaagcgg atgtgtttt taagtatat aaaaaagta tatatgaat aaaatacag cgtgatgaa aaagtaaac ttaagatat acagttaga atacggtat aaaatgaat atcaatgta atatacaat acgtacata atatact
- >TC009743 RB
ggtgtcgtc agcgtcgtc agttgtcag tagtaagtc aaaatggct gccagcgat gaagagcct atgcatcat ttatatgag gtttttaca aattgtttt aataaaatc gcaaacaag gcaccagag aaacccggt tttcagtcg ccatatggt ggaattgac aacggaatg gcctacgcc ataagtccg gcgggtccg gtttatacg ggtcccgga gccagtccg acacaagcc acaggagaa ccgttcgca gcagatagc ccttatttt ccattcagt gctaggccg ggaagtaga ctatcgcct gcgaaacct aaagccggc cttaggccg accaaagaa gaaccgcca cagggaaat tcgatcgac aaccagtgg tatggttcg gatgaattc aacgactcg ccccgctac acctcgccc aaccctacg acgggttac tatctgggc gcggatgca acccccggc gaatggcac ccttcgtac accacaggc gcgtaccaa acgtacgaa gcgtacggt ctcatccca gaaacagaa ggtcaaggt ctgcccccg atgtcttcg ttaaggccg aacggtacc gccgtgacc accatgtcc ggtacaaac agttcggcc ttcggtccg gcaaatcac accgacggt gtgaacaaa gctttagga acaatttat ccaatagtg gaacaagct agcgctagt agttatagt tcggccccg tcgacgccg gttagttcg ccgcctcca ttagcgcca tctagctgg ttagccaat cataacact ccacattcg ccccattat acacaaacg gttaatgtg aataatagt gctgcacct cagggttta catatgcct cccgccccc gaagcgcaa cgcttagat gatgcaatt gggttttta agagaccac gcggaagta aatggaaca cgtatggag gaaagactt gacgacgca attaacgta ttgcgtaat cacgcggaa ccacagatc atagggcag cttccacca cctcaccag atgacgaca ctgggctac acggcggca gcctcggaa ccacacata ccagacaca gttaaagta gaacgggcg acatacaat acaaaaaag cgcaaagag cctccagat agcgacacc aaaccatcc agttcagta gaaacgata gtacaacca aattcaacc agtggtcct ccaccaaaa acgacgaag aggtcgcgg agatataat ggtgacgaa gatgaaatt gatgatcca agcaacaag gccgtacgc gagaaagag cgtaggcag gccaacaat gtccgtgaa cgttgttcc agtgcggac gaaacggaa gagggtgaa ctagatccc gatattaaa gcgcaaagg gagaaagag aggaggcaa gccaataac gctagagaa aggatacgt attcgtgat attaacgaa gctttaaaa gaactggga agaatgtgc atggcgcat ctaaagacg gacaaacca caaacaaag cttggtatc ctaaatatg gctgtcgaa gtcattatg actttagaa cagcaagtt agagagcga aacttgaat cctaaggcg gcatgtctg aagcggcga gaggaagaa aaggcagaa gatggccct aaattaggg ccagggccg catcatatg cttactgcc caacattac cccacctta ccgggtgct cctgccaac ttgcctcac aatccaggc cagccccaa tagcactgc taaaacggc aggtctgcc acctgtaca taacttttc gtacaaacg gctctcatc atttgttca tcaatgatc atgcaaaca aactatatt atatttttt taagaatgt tctgtagtg atacaataa cgtatttta ttgttttat aaattactt ttcacttta ttgagatgg aaaaacgag aggtttgac gacattttt ttaccgagc gtatcgacg cacgaccgg agcagtttg tttcttttt ttttaatac atgtcgaac aattttgtg tgtgccaaa tataagaaa cacaatact cttaccgtg aaacaaata cgtacgttt ttgatctct ctttgccat gatttttgt gacatattt tcgtagttc gtagcaaaa ttgtgccat tcgttttga atttttttc gccttttaa tgataatgc gagaaaaat tcgatcggt actccgatc gtgcgttga ccgaaattg atacatgcg ccagtattt tgaaaatta tgacttata gaattttta tgggaagtc gtcttctca tcacaaact gtaaataag tgttcatta gtgtgaccg gtgacgttt ctccagtat tttgatatg caatataaa atttaactt acgtattac gaaaagggg agaggtttc cggtgctct cttaaatat atgccattg aaaaatgaa cataactta agatattac ttatttaat attaaacag tctgtttat tcactgaac tattttttt atcttatat attttatta tttgagcgg tagattttt ttaattatt ctactaaac ttagtgcaa cttttaaat ttagtttgg tgttcaaac tataaccag atatatcga taaattttt gttcattat ttttaattt taagtttta tgtaatatt taactataa tttttatta ggtatattt tactttaag gtaaagatg taattttgc attgtttct cttttagct ttttagtga atctttatt tatatttgt ttttattgt taaaaaagc tcagtttga atttcgacg gagttcaac cgacggttt attgttgca atgcaatca ggatttagc gtcattgta aattttttg caataactc gtaatggca tagtatatt tttcattcc accggcagc cattttgta ctattattt attaggcag caaatggaa gacattaat cgcggcagc cgtgcataa acacaatca ttcatcttc tattttggc tcaattttc aatattggt gcaactaat ttttttgtt tttgtatta ttctaaaca ttttgtgaa acgaatctt gatagttaa cattgttgt ctaccatta cacaaattg tttagacgt ttgataatg atatatatt tttttagtt agtggacaa catgttatt cttgagatt tggctctta aacgattag acgatttgt aaagcttac cttgacatg tataaacgt ttaaagcgg atgtgtttt taagtatat aaaaaagta tatatgaat aaaatacag cgtgatgaa aaagtaaac ttaagatat acagttaga atacggtat aaaatgaat atcaatgta atatacaat acgtacata atatact
- >TC009743 RC
ggtgtcgtc agcgtcgtc agttgtcag tagtaagtc aaaatggct gccagcgat gaagagcct atgcatcat ttatatgag gtttttaca aattgtttt aataaaatc gcaaacaag gcaccagag aaacccggt tttcagtcg ccatatggt ggaattgac aacggaatg gcctacgcc ataagtccg gcgggtccg gtttatacg ggtcccgga gccagtccg acacaagcc acaggagaa ccgttcgca gcagatagc ccttatttt ccattcagt gctaggccg ggaagtaga ctatcgcct gcgaaacct aaagccggc cttaggccg accaaagaa gaaccgcca cagggaaat tcgatcgac aaccagtgg tatggttcg gatgaattc aacgactcg ccccgctac acctcgccc aaccctacg acgggttac tatctgggc gcggatgca acccccggc gaatggcac ccttcgtac accacaggc gcgtaccaa acgtacgaa gcgtacggt ctcatccca gaaacagaa ggtcaaggt ctgcccccg atgtcttcg ttaaggccg aacggtacc gccgtgacc accatgtcc ggtacaaac agttcggcc ttcggtccg gcaaatcac accgacggt gtgaacaaa gctttagga acaatttat ccaatagtg gaacaagct agcgctagt agttatagt tcggccccg tcgacgccg gttagttcg ccgcctcca ttagcgcca tctagctgg ttagccaat cataacact ccacattcg ccccattat acacaaacg gttaatgtg aataatagt gctgcacct cagggttta catatgcct cccgccccc gaagcgcaa cgcttagat gatgcaatt gggttttta agagaccac gcggaagta aatggaaca cgtatggag gaaagactt gacgacgca attaacgta ttgcgtaat cacgcggaa ccacagatc atagggcag cttccacca cctcaccag atgacgaca ctgggctac acggcggca gcctcggaa ccacacata ccagacaca gttaaagta gaacgggcg acatacaat acaaaaaag cgcaaagag cctccagat agcgacacc aaaccatcc agttcagta gaaacgata gtacaacca aattcaacc agtggtcct ccaccaaaa acgacgaag aggtcgcgg agatataat ggtgacgaa gatgaaatt gatgatcca agcaacaag gccgtacgc gagaaagag cgtaggcag gccaacaat gtccgtgaa cgttgttcc agtgcggac gaaacggaa gagggtgaa ctagatccc gatattaaa gcgcaaagg gagaaagag aggaggcaa gccaataac gctagagaa aggatacgt attcgtgat attaacgaa gctttaaaa gaactggga agaatgtgc atggcgcat ctaaagacg gacaaacca caaacaaag cttggtatc ctaaatatg gctgtcgaa gtcattatg actttagaa cagcaagtt agagagcga aacttgaat cctaaggcg gcatgtctg aagcggcga gaggaagaa aaggcagaa gatggccct aaattaggg ccagggccg catcatatg cttactgcc caacattac cccacctta ccgggtgct cctgccaac ttgcctcac aatccaggc cagccccaa tagcactgc taaaacggc aggtctgcc acctgtaca taacttttc gtacaaacg gctctcatc atttgttca tcaatgatc atgcaaaca aactatatt atatttttt taagaatgt tctgtagtg atacaataa cgtatttta ttgttttat aaattactt ttcacttta ttgagatgg aaaaacgag aggtttgac gacattttt ttaccgagc gtatcgacg cacgaccgg agcagtttg tttcttttt ttttaatac atgtcgaac aattttgtg tgtgccaaa tataagaaa cacaatact cttaccgtg aaacaaata cgtacgttt ttgatctct ctttgccat gatttttgt gacatattt tcgtagttc gtagcaaaa ttgtgccat tcgttttga atttttttc gccttttaa tgataatgc gagaaaaat tcgatcggt actccgatc gtgcgttga ccgaaattg atacatgcg ccagtattt tgaaaatta tgacttata gaattttta tgggaagtc gtcttctca tcacaaact gtaaataag tgttcatta gtgtgaccg gtgacgttt ctccagtat tttgatatg caatataaa atttaactt acgtattac gaaaagggg agaggtttc cggtgctct cttaaatat atgccattg aaaaatgaa cataactta agatattac ttatttaat attaaacag tctgtttat tcactgaac tattttttt atcttatat attttatta tttgagcgg tagattttt ttaattatt ctactaaac ttagtgcaa cttttaaat ttagtttgg tgttcaaac tataaccag atatatcga taaattttt gttcattat ttttaattt taagtttta tgtaatatt taactataa tttttatta ggtatattt tactttaag gtaaagatg taattttgc attgtttct cttttagct ttttagtga atctttatt tatatttgt ttttattgt taaaaaagc tcagtttga atttcgacg gagttcaac cgacggttt attgttgca atgcaatca ggatttagc gtcattgta aattttttg caataactc gtaatggca tagtatatt tttcattcc accggcagc cattttgta ctattattt attaggcag caaatggaa gacattaat cgcggcagc cgtgcataa acacaatca ttcatcttc tattttggc tcaattttc aatattggt gcaactaat ttttttgtt tttgtatta ttctaaaca ttttgtgaa acgaatctt gatagttaa cattgttgt ctaccatta cacaaattg tttagacgt ttgataatg atatatatt tttttagtt agtggacaa catgttatt cttgagatt tggctctta aacgattag acgatttgt aaagcttac cttgacatg tataaacgt ttaaagcgg atgtgtttt taagtatat aaaaaagta tatatgaat aaaatacag cgtgatgaa aaagtaaac ttaagatat acagttaga atacggtat aaaatgaat atcaatgta atatacaat acgtacata atatact
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