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Gene information
General information
TC009740 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7138571..7141297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009740
    iBeetle screen iB_02848

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009740 RA
      MILLPESLS AMQKYEKLE KIGEGTYGT VFKAKNRET 
      HEIVALKRV RLDDDDEGV PSSALREIC LLKELKHKN 
      IVRLYDVLH SDKKLTLVF EHCDQDLKK YFDSLNGDI 
      DLDVVKSFM YQLLRGLAF CHSHNVLHR DLKPQNLLI 
      NKNGELKLA DFGLARAFG IPVKCYSAE VVTLWYRPP 
      DVLFGAKLY TTSIDMWSA GCIFAELAN AGRPLFPGS 
      DVDDQLRRI FKLLGTPTE ETWSGMTQL PDYKPFPLY 
      QPNMSLSQV VPKLGNRGR DLLQRLLVC NPMGRMSAD 
      DAMAHAYFS DLNPAIKND RC
    CDS sequence
    • >TC009740 RA
      atgattctg ctacccgag agcctttcc gcgatgcag 
      aaatatgaa aagttggag aaaatcggc gaaggcact 
      tacggcacc gtctttaag gcaaaaaac cgcgaaacc 
      cacgaaatc gtagctctc aaaagggtg cgcttggac 
      gacgatgac gagggggtg ccttcctca gctttacgg 
      gaaatatgc ttactgaag gaactcaaa cacaaaaac 
      atcgtccgt ttatatgat gttctccac agtgataag 
      aaattgact ctagttttt gaacactgt gatcaagat 
      ctcaagaag tatttcgat agcttgaac ggtgatatt 
      gacttagac gtagttaaa tccttcatg taccagtta 
      ctaagaggt ctagcgttc tgccatagt cataatgtg 
      ttacacaga gacctgaaa ccacaaaat ttgctcatt 
      aacaaaaac ggcgagttg aagttggcc gactttggg 
      ctggctagg gcgttcgga atcccggtg aagtgttac 
      tcggccgaa gtggtcacc ttgtggtac cggcccccg 
      gatgtcttg tttggggcc aaactgtac accacttcc 
      attgacatg tggtcagcg gggtgtatt tttgcggaa 
      ttggcaaat gccggtcga cccttattc ccgggttcc 
      gatgttgat gaccagttg agacggatt tttaaatta 
      ctagggacc cccactgaa gaaacttgg agcggaatg 
      actcaactt cccgattat aaaccgttt ccgctctat 
      cagcctaac atgagttta tctcaggtt gtgcctaaa 
      ttagggaac agaggtaga gatcttctg cagagacta 
      ctagtttgt aaccccatg ggaaggatg tccgcagac 
      gacgctatg gcgcacgct tatttcagt gatttaaat 
      ccagcaatt aaaaatgac cgatgctaa
    mRNA sequence
    • >TC009740 RA
      caagtgtca aaaacatca aaacaaaca taacctcca 
      aatagtgcc gaaaacctg aattcggtg aatgattct 
      gctacccga gagcctttc cgcgatgca gaaatatga 
      aaagttgga gaaaatcgg cgaaggcac ttacggcac 
      cgtctttaa ggcaaaaaa ccgcgaaac ccacgaaat 
      cgtagctct caaaagggt gcgcttgga cgacgatga 
      cgagggggt gccttcctc agctttacg ggaaatatg 
      cttactgaa ggaactcaa acacaaaaa catcgtccg 
      tttatatga tgttctcca cagtgataa gaaattgac 
      tctagtttt tgaacactg tgatcaaga tctcaagaa 
      gtatttcga tagcttgaa cggtgatat tgacttaga 
      cgtagttaa atccttcat gtaccagtt actaagagg 
      tctagcgtt ctgccatag tcataatgt gttacacag 
      agacctgaa accacaaaa tttgctcat taacaaaaa 
      cggcgagtt gaagttggc cgactttgg gctggctag 
      ggcgttcgg aatcccggt gaagtgtta ctcggccga 
      agtggtcac cttgtggta ccggccccc ggatgtctt 
      gtttggggc caaactgta caccacttc cattgacat 
      gtggtcagc ggggtgtat ttttgcgga attggcaaa 
      tgccggtcg acccttatt cccgggttc cgatgttga 
      tgaccagtt gagacggat ttttaaatt actagggac 
      ccccactga agaaacttg gagcggaat gactcaact 
      tcccgatta taaaccgtt tccgctcta tcagcctaa 
      catgagttt atctcaggt tgtgcctaa attagggaa 
      cagaggtag agatcttct gcagagact actagtttg 
      taaccccat gggaaggat gtccgcaga cgacgctat 
      ggcgcacgc ttatttcag tgatttaaa tccagcaat 
      taaaaatga ccgatgcta aattttttt attaatttt 
      gaaacgtcc attctctag tcataacca attttttta 
      ttatgttta tttggagat tataattcg gtgtaaaca 
      tagccatct cactttcaa taaacgtac tgaaactga 
      ggtgactat ttttcatta ccattggat tttttcgtg 
      atgtaaacc atttttata ttgaaataa ttgtaggaa 
      taaacaact catttgaat tacattatt tacgaaccc 
      ttgcgtatt ttgcgggaa aaattggag aggtgcaga 
      taaaatgcg tttttttaa taatttatt acagcaata 
      ctaagcaca ataacaaag attaggaga ttgacagga 
      cacgtaagt acgacgaaa taagtaact gatatactt 
      tggccctta gatatctgg cacacaata tgtttgcat 
      ggcaattac gtatttcct atatcgaaa tataactag 
      acagttcta tgtttaata aagagaaca tgcactttg 
      cactgcata acaatatga aatacatgt tcgacctac 
      gaggattcc ctcgggtct gagcatttt aattataca 
      caaaaattc aggccggac gttttaaca ctgtaataa 
      aatcgcgtg taagtaaac aaaacaaac attaaaaca 
      tagcaaaat aaagcacga atctaaaac ctaacacaa 
      aaacgtgaa agtatcaca cggaaatac caagagtca 
      gtcttttat cacaatgta cactacaaa cttaattaa 
      aattcaagt gcaatctga ccaaagcct ttaaaaata 
      tttcatcca gggcgaggt tcaccagat ccgattggt 
      gagactagc tctaacgat gtaacaccg ctaagtggt 
      gactaagtt ttattaaac tttgattaa ggaacatta 
      gttcgttta aaaagacag ttatttact tcatctaaa 
      aataaagaa cataaacat tctcaaata aaacaacaa 
      attgcaaat ttatcactc ggaaaatga ctaatcgac 
      gtcaattca atttcagac taataaata tcttaaacc 
      tacttaata atttataac atgaatttc atacaaact 
      tacaaacgc cataacaac ttagatatt cctaaactg 
      ccattacga aatgatttg tctttaata aatcggtat 
      ttaaaacac gttcccgtt ttatcattt ttagtttag 
      atccacatc ccttgagcc ttgcgttcg aaaataaaa 
      caaatacaa ttctgcaag ataattcaa atttttaca 
      taatgttat ctagacatt tattattaa tatctaaat 
      atagcgcag tgttttaac tgcagatat tttcaatgt 
      acactttaa caatttgtt acatctggg acaactttg 
      tcagacgac ttctcccct aaaaaatat cttcataaa 
      tacttcaac tttgtagct ccacttttg cagcagcgg 
      cggcttttt gtttctcga tgtagcgtt ttctggcgc 
      tctgaatca aattctctt tgcgtctgt ggagaatct 
      tttcgcgct ccgacgaag acttcgaaa atctgctcc 
      ccaaactgc tcgtatctt cactgaaaa agcacaatc 
      ttcaatatt tctatattg gctatgcaa ccagtaaaa 
      cacttctgt caagattaa taattttgt ctccatgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY