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Gene information
General information
TC033649
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009727
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 2856918..2860594
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033649
    iBeetle screen iB_02845
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Pupal morphology
    wings blistered - Number of animals showing the phenotype: 9

    elytra surface irregular - Number of animals showing the phenotype: 9

    Comment: wing veins potentially not present, surface smoothed (secondary defect?)
    Adult morphology
    wings irregular - Number of animals showing the phenotype: 7


    wings not closed - Number of animals showing the phenotype: 7


    wings blistered - Number of animals showing the phenotype: 7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033649 RA
      MWWLLLSLV WWCGTCAIP SPGPEVWKC PKISEQPVV 
      ECSCDMPHT LRCTGDKSA MSIIAKTLR LHNAADVSL 
      LDCTVQNVS AISGPLLEG ISLHGLVIS SGEIRDIDE 
      SAFKGLTAP LQALGLPNN KLATVPTQA LKPLPELDR 
      LDLSSNKLK SLEATSFKG LRNLSFIDL SDNMLTKIV 
      PNTFDDLPQ LKILRLRGN RLTIQTITK LNPLRTVEE 
      IDLSGNNLV GPLGPKTFP KMENLRDIQ LSHNSLSSI 
      KMGALQGLN KLTSLSLQH NQIDVLEDH AFSHLTSLV 
      SLVLAHNRI VAVSGASLA HLNKLTDLD LRFNFLRAL 
      TADLILPLK SLKNLKLDD NDISMVASD ALKETTILK 
      HLTLSENPL NCDCSLTEF AIWLSNSSL PTEDKASAV 
      CTTPPSLEN GLLVEVPLE YLLCGEDEQ ETLMGPLYP 
      PYKTKINLN SFKYDGETI KLQWNIEDE ASTYICDAI 
      IVYEEDGDN EVLVESNPL KCNSSHLPN PKILNVTVP 
      NTIQLQQEH RYRYCVVLF ESGQTDDSS LILGCSDII 
      PLIRNAEIQ PQPQHNIKY PKVLAIQAN LTNYGSLSI 
      NVKIYPQVK CELNLAILE QSALLSQRK INCSDPKYV 
      FVGLDEGPY RVCANVIRP GPAMDVAKS RCVTVFRKE 
      LHGFTTLDI VFVSVFFVL CFIVIALIW GVRRVLLKP 
      KIQTHQCFM PPEGEEQQH NRYVKLQAT TKL
    CDS sequence
    • >TC033649 RA
      atgtggtgg ctgctgctg tcactggtg tggtggtgc 
      ggcacttgc gccatcccg tccccgggc cctgaagtg 
      tggaaatgt ccgaaaatt tcagaacag cccgtggtg 
      gaatgcagc tgcgacatg cctcacacc ttgcgctgc 
      actggcgac aagtcagca atgtcaatc atagcaaaa 
      acactgaga ttgcacaat gcggctgat gtttccctt 
      ttggactgc acagttcaa aacgtgagc gccatttcc 
      gggcctttg ctggaaggg atttccctt cacggccta 
      gtcatatct tctggggaa attagggac attgacgag 
      tcggccttt aagggctta acagcgccc ttgcaagcc 
      ttgggactc cccaacaat aaactcgca acggtgccg 
      actcaagca ctcaagcct ctgccggaa ctcgacaga 
      ctggacttg tccagcaac aagttgaaa agtctggaa 
      gccacctct tttaaaggc ttgcggaac ttgtcgttt 
      atcgatctg agcgacaac atgttgacg aaaattgtg 
      ccaaatact ttcgacgat cttccgcag ttgaagata 
      ttgagactg agaggaaac cgcctcact atacaaact 
      attacgaaa ttgaatccg cttcgaacc gtcgaagaa 
      atcgactta tcagggaat aatttagtc ggaccgttg 
      ggtcctaaa acctttccg aagatggaa aatttgcgc 
      gacatccaa ctatcgcac aactcgctc agtagcatt 
      aaaatgggg gccctgcaa ggcttaaat aaactgact 
      tcgctctcc ttgcaacac aaccagatc gacgtgctt 
      gaagaccac gctttcagt cacttgacg tctttagtc 
      agccttgtg ttggcccat aaccgcatt gtcgcagtg 
      tctggggcc tctctagcc catttaaac aaactgaca 
      gatttggac ttgaggttt aattttctc cgtgccttg 
      actgcagac ttaattttg ccgctgaaa agtctgaaa 
      aacttgaaa cttgacgac aatgatatt tccatggtg 
      gctagcgat gctttaaaa gaaaccaca atcttgaaa 
      cacctgact ttatccgaa aacccgctc aattgtgac 
      tgtagtctg actgaattt gcgatttgg ttgagtaat 
      tcgagtttg ccgaccgaa gacaaggcg agtgctgtc 
      tgcaccact ccaccgtcc ctcgaaaac ggattattg 
      gtggaagta ccgctggaa tacctcctg tgtggggaa 
      gacgaacaa gagactctc atgggccct ttatacccg 
      ccatacaag acaaaaatt aatctgaac tcgtttaaa 
      tacgacggc gagacgatc aaactacag tggaatatt 
      gaagacgaa gcttccacc tacatttgc gacgcaata 
      atcgtgtat gaagaggat ggagataac gaagttttg 
      gtcgaatct aacccatta aaatgcaac tcatcgcat 
      ctcccaaac ccaaaaatc ctgaacgtt acagtcccg 
      aacacgatc cagttgcaa caagaacac agatacaga 
      tactgcgtt gttttgttt gaatcaggc caaacggac 
      gattcctcc ctcatcctg ggctgttct gacattatc 
      cccctaatc cggaacgca gaaatccag ccccagccg 
      caacacaac atcaaatac cccaaggtt ttggccatc 
      caggccaat ctaaccaac tatgggagt ctatcaatt 
      aatgtgaag atctaccca caggtgaaa tgcgaactg 
      aacttggcg attctggag cagagcgct ttgctctcg 
      cagcggaaa ataaactgt agtgaccct aagtacgtg 
      tttgtgggg ctagatgag ggaccgtac agagtgtgt 
      gctaacgtt ataaggcct ggaccggcc atggacgtg 
      gccaagtcc cggtgtgtg acagttttt cgtaaagaa 
      cttcacggt tttacaact ttagacatt gttttcgtg 
      tcggtgttt ttcgtgttg tgtttcatt gtgatagcg 
      ctaatttgg ggggtcagg agagttttg ttgaagcct 
      aagatacag acgcatcag tgttttatg ccgcctgag 
      ggtgaggag caacagcat aacagatac gttaagctt 
      caagctact acgaaatta taa
    mRNA sequence
    • >TC033649 RA
      tacttccaa tgaaccggg atgtggtgg ctgctgctg 
      tcactggtg tggtggtgc ggcacttgc gccatcccg 
      tccccgggc cctgaagtg tggaaatgt ccgaaaatt 
      tcagaacag cccgtggtg gaatgcagc tgcgacatg 
      cctcacacc ttgcgctgc actggcgac aagtcagca 
      atgtcaatc atagcaaaa acactgaga ttgcacaat 
      gcggctgat gtttccctt ttggactgc acagttcaa 
      aacgtgagc gccatttcc gggcctttg ctggaaggg 
      atttccctt cacggccta gtcatatct tctggggaa 
      attagggac attgacgag tcggccttt aagggctta 
      acagcgccc ttgcaagcc ttgggactc cccaacaat 
      aaactcgca acggtgccg actcaagca ctcaagcct 
      ctgccggaa ctcgacaga ctggacttg tccagcaac 
      aagttgaaa agtctggaa gccacctct tttaaaggc 
      ttgcggaac ttgtcgttt atcgatctg agcgacaac 
      atgttgacg aaaattgtg ccaaatact ttcgacgat 
      cttccgcag ttgaagata ttgagactg agaggaaac 
      cgcctcact atacaaact attacgaaa ttgaatccg 
      cttcgaacc gtcgaagaa atcgactta tcagggaat 
      aatttagtc ggaccgttg ggtcctaaa acctttccg 
      aagatggaa aatttgcgc gacatccaa ctatcgcac 
      aactcgctc agtagcatt aaaatgggg gccctgcaa 
      ggcttaaat aaactgact tcgctctcc ttgcaacac 
      aaccagatc gacgtgctt gaagaccac gctttcagt 
      cacttgacg tctttagtc agccttgtg ttggcccat 
      aaccgcatt gtcgcagtg tctggggcc tctctagcc 
      catttaaac aaactgaca gatttggac ttgaggttt 
      aattttctc cgtgccttg actgcagac ttaattttg 
      ccgctgaaa agtctgaaa aacttgaaa cttgacgac 
      aatgatatt tccatggtg gctagcgat gctttaaaa 
      gaaaccaca atcttgaaa cacctgact ttatccgaa 
      aacccgctc aattgtgac tgtagtctg actgaattt 
      gcgatttgg ttgagtaat tcgagtttg ccgaccgaa 
      gacaaggcg agtgctgtc tgcaccact ccaccgtcc 
      ctcgaaaac ggattattg gtggaagta ccgctggaa 
      tacctcctg tgtggggaa gacgaacaa gagactctc 
      atgggccct ttatacccg ccatacaag acaaaaatt 
      aatctgaac tcgtttaaa tacgacggc gagacgatc 
      aaactacag tggaatatt gaagacgaa gcttccacc 
      tacatttgc gacgcaata atcgtgtat gaagaggat 
      ggagataac gaagttttg gtcgaatct aacccatta 
      aaatgcaac tcatcgcat ctcccaaac ccaaaaatc 
      ctgaacgtt acagtcccg aacacgatc cagttgcaa 
      caagaacac agatacaga tactgcgtt gttttgttt 
      gaatcaggc caaacggac gattcctcc ctcatcctg 
      ggctgttct gacattatc cccctaatc cggaacgca 
      gaaatccag ccccagccg caacacaac atcaaatac 
      cccaaggtt ttggccatc caggccaat ctaaccaac 
      tatgggagt ctatcaatt aatgtgaag atctaccca 
      caggtgaaa tgcgaactg aacttggcg attctggag 
      cagagcgct ttgctctcg cagcggaaa ataaactgt 
      agtgaccct aagtacgtg tttgtgggg ctagatgag 
      ggaccgtac agagtgtgt gctaacgtt ataaggcct 
      ggaccggcc atggacgtg gccaagtcc cggtgtgtg 
      acagttttt cgtaaagaa cttcacggt tttacaact 
      ttagacatt gttttcgtg tcggtgttt ttcgtgttg 
      tgtttcatt gtgatagcg ctaatttgg ggggtcagg 
      agagttttg ttgaagcct aagatacag acgcatcag 
      tgttttatg ccgcctgag ggtgaggag caacagcat 
      aacagatac gttaagctt caagctact acgaaatta 
      taaaaagcc tcttgttgt tcgatttat aattgtgat 
      gtaggtatc taatgactt ttagtgcca tataaatta 
      aggttttgt tgagtgcaa atacgtcaa tttttaagt 
      aaattactc gatgtaaaa tatattttt acagtgtac 
      atatagtca ctcttacat ttataaaaa cataggttt 
      taaatagtg gttacgtct gtttgaaaa tgttataca 
      aaccttgct tgctgctgg ataattaca ataacttgt 
      gcatgtgtg aattaagag aaatatagt tttttctaa 
      tttttaaag aggtggtca ttgttaaat taaacatat 
      ctcgtctgt tattaatta tccaaattt attttattc 
      aaagaatta atggtgcta aaaattaaa tacactttt 
      tatttaacg atatgtttt tactctatg taggtctag 
      tgtagtttc gtccacttt tttgtaaat aactaactg 
      tgttaattg ttatcttaa tgccgttta aactaattc 
      gctgattta atgaattta gaggcaagt agaaatgtt 
      ctaagtaag tttcttgta aataacttt gtactacta 
      atgcttgtt ttcattttt tgttatact tttattaaa 
      caaatattt atgcactgc cttgtaaaa taaaatatt 
      taatattta