Status
Please wait ...
Gene information
General information
TC033636 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4703064..4705977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033636
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02842

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033636 RA
      MKLTCSRTN PLLGGLIAA EYVKILKNV SLEVQWGDA 
      NVVDNCSKK IICDTNNDL LRAIARLFA ENELYGGTP 
      LEKTEVDHW LSYSLSLKT SKNDLKDLL TLLDKSLQA 
      VTFLACKRL TIADFAVFA SLYSSRPWQ DLISRNETP 
      VNVLRWYNF IQSQPAVVQ ILKSLPNEA KSALVPPAG 
      RQSLDKSNV GNRAQEGKF VELPGAEMG KVVVRFPPE 
      ASGYLHIGH AKAALLNQY YQEAFKGKL VMRFDDTNP 
      AKENLHFEK VILEDVALL ELKPDMFTH TSQYFDLML 
      EFCEKLLKE GKAYVDDTD PETMKMQRE QKIEAASRN 
      NTYEQNSKL WEEMKKGTQ KGQQCCVRA KIDMQSPNG 
      CLRDPTIYR CKNEPHPRT GDKYKVYPT YDFACPIVD 
      SIEGVTHVL RTMEYHDRD PQFYWFIEA LGLRRPYIW 
      EYSRLSMTN TVLSKRKLT WFVNEGLVD GWDDPRMPT 
      VRGVLRRGM TVQGLKEFI IAQGSSRSV VFMEWDKIW 
      AFNKKVIDP IAPRYTAVE FKDPVTITV KGVKEECLT 
      VPKHPKNTD VGNKSVWIG PKIIIDRVD AECLKEGEN 
      ATFINWGNL LIKKINKEK NVITSIEAE PNLDNKDYK 
      KTLKLTWLA VTDNAKFTP TWCVYFDHL ISKALLGKD 
      EDFKQFIGH ETRKEFQMI GDPELKKLK KGDIIQLQR 
      RGFFRVDVA YEPMDLVTC KEQPVILFY IPDGHTKES 
      PFALNTAKA TTNAVSFLL F
    CDS sequence
    • >TC033636 RA
      atgaagctg acttgtagc agaactaac ccgctttta 
      ggcggttta attgcggcc gaatatgta aaaatctta 
      aagaacgtt tcactggaa gtgcagtgg ggcgatgcc 
      aacgttgtt gataactgc tccaaaaaa attatttgc 
      gatactaac aatgattta ttgagggcc attgcgcga 
      ttattcgcc gaaaatgag ttatacgga ggcactcca 
      cttgaaaaa actgaagtc gaccactgg ttgagttac 
      tcactgagt ctaaaaaca tcaaaaaac gatcttaaa 
      gacctttta actttgctg gataagtcg ctacaagcg 
      gtcacgttt ctggcctgt aaacggcta acaattgcc 
      gattttgca gtttttgca agtctttac agttccaga 
      ccttggcaa gacctgatt tcgcgaaac gaaacccct 
      gttaatgtt ttaaggtgg tacaatttt atccagtca 
      cagccagct gttgtgcag attttgaaa tcattgcct 
      aatgaagct aaatcagcg ttggtacca ccagcaggg 
      agacagtct ttagataag tcaaatgtg ggcaatagg 
      gcacaagag ggcaagttt gttgagttg ccaggggcg 
      gaaatgggc aaggtcgta gtcagattc ccacctgaa 
      gcttcagga tatctgcat attggtcac gcaaaggcc 
      gccctcctt aaccagtat taccaagag gccttcaag 
      ggaaagctt gttatgcgt tttgatgac acaaatcca 
      gctaaggag aatctacac tttgaaaaa gtgatcctt 
      gaagatgtt gctttattg gagctgaaa ccggatatg 
      ttcactcat acttcgcag tattttgat ttaatgttg 
      gaattttgt gagaagctg ttgaaagag ggcaaggca 
      tatgtggac gatactgat cctgagacc atgaaaatg 
      caacgcgaa caaaaaatt gaagcggct agtagaaat 
      aatacttac gagcagaat tcaaaattg tgggaggaa 
      atgaaaaaa gggacacag aaaggccaa cagtgttgt 
      gtgagggcg aaaattgac atgcagtcg cccaatggg 
      tgtctcagg gaccccaca atttacagg tgcaagaac 
      gaaccccac cccagaact ggagacaaa tataaagta 
      tatccgacg tacgatttt gcctgtcct attgtggac 
      tccattgag ggtgtgacc catgtgtta cgaaccatg 
      gagtaccat gaccgtgat ccccaattc tattggttt 
      attgaagct ttaggttta cgcagacct tacatttgg 
      gagtacagc agactctca atgaccaac actgttcta 
      agtaaacga aaattgact tggtttgtc aatgagggg 
      cttgtggat ggatgggat gatcccaga atgcctaca 
      gttagaggg gtgctgagg agagggatg actgttcaa 
      ggcttgaaa gaatttatc atagctcaa ggttcaagt 
      cgttcggtt gttttcatg gagtgggac aaaatctgg 
      gcctttaat aaaaaagtt atcgatccg attgccccc 
      cgttacact gcggtggaa ttcaaagat cctgttaca 
      atcacagtg aaaggggtt aaagaagag tgtcttact 
      gttcccaaa catccgaaa aatacagac gttgggaat 
      aaatcagtg tggattgga ccgaaaatt attattgat 
      cgagtcgat gctgagtgt ttgaaagaa ggggaaaat 
      gcgactttt atcaattgg gggaatttg ctcattaaa 
      aaaattaac aaggaaaaa aatgtaatt acatcgatc 
      gaggctgag ccaaacttg gataataaa gactataag 
      aaaacactg aaattaacg tggttggcg gtcacagat 
      aatgcgaag tttacgcca acgtggtgc gtttatttt 
      gatcattta attagtaag gcgcttttg ggcaaagac 
      gaagatttc aaacagttt attggacat gagacgcga 
      aaagaattt caaatgatt ggtgatcct gaattaaaa 
      aaattgaaa aaaggggat attattcaa ttgcaacga 
      cgcggtttc tttagagtt gatgttgca tacgaacct 
      atggatttg gtgacttgt aaagagcag ccagttatt 
      ttgttctat attcctgat gggcatacg aaggagagc 
      ccgtttgcg cttaatact gccaaggcc acgactaat 
      gcggtaagt tttttatta ttttaa
    mRNA sequence
    • >TC033636 RA
      tgcatgtgt gagatttcg ttgcacaat atttcttga 
      atttttcaa taaaaaaat aagtttggt tggttttga 
      gtgttgttt ttgtgttgc cggtgcatc atgaagctg 
      acttgtagc agaactaac ccgctttta ggcggttta 
      attgcggcc gaatatgta aaaatctta aagaacgtt 
      tcactggaa gtgcagtgg ggcgatgcc aacgttgtt 
      gataactgc tccaaaaaa attatttgc gatactaac 
      aatgattta ttgagggcc attgcgcga ttattcgcc 
      gaaaatgag ttatacgga ggcactcca cttgaaaaa 
      actgaagtc gaccactgg ttgagttac tcactgagt 
      ctaaaaaca tcaaaaaac gatcttaaa gacctttta 
      actttgctg gataagtcg ctacaagcg gtcacgttt 
      ctggcctgt aaacggcta acaattgcc gattttgca 
      gtttttgca agtctttac agttccaga ccttggcaa 
      gacctgatt tcgcgaaac gaaacccct gttaatgtt 
      ttaaggtgg tacaatttt atccagtca cagccagct 
      gttgtgcag attttgaaa tcattgcct aatgaagct 
      aaatcagcg ttggtacca ccagcaggg agacagtct 
      ttagataag tcaaatgtg ggcaatagg gcacaagag 
      ggcaagttt gttgagttg ccaggggcg gaaatgggc 
      aaggtcgta gtcagattc ccacctgaa gcttcagga 
      tatctgcat attggtcac gcaaaggcc gccctcctt 
      aaccagtat taccaagag gccttcaag ggaaagctt 
      gttatgcgt tttgatgac acaaatcca gctaaggag 
      aatctacac tttgaaaaa gtgatcctt gaagatgtt 
      gctttattg gagctgaaa ccggatatg ttcactcat 
      acttcgcag tattttgat ttaatgttg gaattttgt 
      gagaagctg ttgaaagag ggcaaggca tatgtggac 
      gatactgat cctgagacc atgaaaatg caacgcgaa 
      caaaaaatt gaagcggct agtagaaat aatacttac 
      gagcagaat tcaaaattg tgggaggaa atgaaaaaa 
      gggacacag aaaggccaa cagtgttgt gtgagggcg 
      aaaattgac atgcagtcg cccaatggg tgtctcagg 
      gaccccaca atttacagg tgcaagaac gaaccccac 
      cccagaact ggagacaaa tataaagta tatccgacg 
      tacgatttt gcctgtcct attgtggac tccattgag 
      ggtgtgacc catgtgtta cgaaccatg gagtaccat 
      gaccgtgat ccccaattc tattggttt attgaagct 
      ttaggttta cgcagacct tacatttgg gagtacagc 
      agactctca atgaccaac actgttcta agtaaacga 
      aaattgact tggtttgtc aatgagggg cttgtggat 
      ggatgggat gatcccaga atgcctaca gttagaggg 
      gtgctgagg agagggatg actgttcaa ggcttgaaa 
      gaatttatc atagctcaa ggttcaagt cgttcggtt 
      gttttcatg gagtgggac aaaatctgg gcctttaat 
      aaaaaagtt atcgatccg attgccccc cgttacact 
      gcggtggaa ttcaaagat cctgttaca atcacagtg 
      aaaggggtt aaagaagag tgtcttact gttcccaaa 
      catccgaaa aatacagac gttgggaat aaatcagtg 
      tggattgga ccgaaaatt attattgat cgagtcgat 
      gctgagtgt ttgaaagaa ggggaaaat gcgactttt 
      atcaattgg gggaatttg ctcattaaa aaaattaac 
      aaggaaaaa aatgtaatt acatcgatc gaggctgag 
      ccaaacttg gataataaa gactataag aaaacactg 
      aaattaacg tggttggcg gtcacagat aatgcgaag 
      tttacgcca acgtggtgc gtttatttt gatcattta 
      attagtaag gcgcttttg ggcaaagac gaagatttc 
      aaacagttt attggacat gagacgcga aaagaattt 
      caaatgatt ggtgatcct gaattaaaa aaattgaaa 
      aaaggggat attattcaa ttgcaacga cgcggtttc 
      tttagagtt gatgttgca tacgaacct atggatttg 
      gtgacttgt aaagagcag ccagttatt ttgttctat 
      attcctgat gggcatacg aaggagagc ccgtttgcg 
      cttaatact gccaaggcc acgactaat gcggtaagt 
      tttttatta ttttaaaat gaaacaact ttttatctt 
      ttaaattaa ataatttat taaattttt ttaaataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY