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Gene information
General information
TC015925 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 2174294..2183894
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015925
    iBeetle screen iB_02838
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber partially degenerated - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    elytra distal colour irregular - Number of animals showing the phenotype: 2


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015925 RA
      MGAFLDKPK TDKFQECGE GNGLRYGVA SMQGWRVEM 
      EDAHMAKTN LGDALKDWS YFAVFDGHA GAKVSAHCA 
      EHLLDAIMQ TEEFQKDVM KGIHNGFLE LDNKMRSLP 
      EMTSGEDKS GTTAVCAFV SPRLIYVAN CGDSRAVLC 
      RGGSPVFTT QDHKPGLPS ERERIVKAG GNVMIQRVN 
      GSLAVSRAL GDYEYKNVE GRGPCEQLV SPEPEIFVR 
      DRDDKEDEF LVLACDGIW DVMSNEDLC QYIHNRLLV 
      TDNLQEVTS QVIDTCLNK GSRDNMSIV LVVFPGAPT 
      PTQEAIDAE KEFDATLER RIKEIVEQN EITEFSTVL 
      HKLASLNIE GFPPGGGLA AKRTLIEKI FKELCPDLA 
      ETDLDPFN
    CDS sequence
    • >TC015925 RA
      atgggagca tttcttgac aagccaaag acagataaa 
      ttccaagag tgtggagaa ggcaacggg ctgcgctat 
      ggggtcgcc agcatgcag ggctggagg gtcgagatg 
      gaggacgca cacatggcc aagacgaac ctgggggac 
      gccctcaaa gactggtct tacttcgcc gttttcgat 
      ggccatgct ggggccaag gtgtcagcc cactgcgcc 
      gagcacctc ctcgacgcc atcatgcaa acggaggag 
      ttccaaaag gacgtgatg aaaggcatc cacaacggt 
      tttctagag ttagataat aaaatgcgc tcgttacct 
      gaaatgacg tccggcgaa gacaagagc ggcacaacg 
      gccgtctgt gcattcgtt tcacctaga cttatctat 
      gtagcgaat tgcggggat tcccgcgcc gttttatgt 
      cgcggtggc agccccgtt ttcaccaca caagaccac 
      aagcccggg ttgccttcg gaaagggag cgcattgtt 
      aaagcagga ggcaatgtt atgatccag agagtgaac 
      ggaagtttg gccgtatcg agggctcta ggcgattac 
      gagtacaaa aatgtcgaa ggaaggggg ccctgtgag 
      cagttggtt tcgcccgaa ccggaaatc ttcgtcagg 
      gatagagat gataaagag gatgagttt ctggtgttg 
      gcttgcgat ggaatttgg gacgtgatg agcaacgag 
      gatctctgc cagtatata cataatcgc cttttagtc 
      accgataat ttacaggaa gttaccagt caagttatc 
      gatacgtgt ttgaacaaa ggtagccgc gataacatg 
      agtattgtg ttagttgtg tttcctggc gctccgacc 
      cccactcag gaagcaatc gatgccgaa aaggaattc 
      gatgcgact ctggaaagg cgtataaaa gaaattgtg 
      gaacaaaac gaaatcact gaattctcc acggtgctg 
      cataagctt gcatcactc aacattgaa gggtttcct 
      ccaggtggt ggactagct gcaaaacgt acactgatt 
      gagaaaatt ttcaaagaa ctgtgtccg gatctagct 
      gaaactgat ttggaccct ttcaactaa
    mRNA sequence
    • >TC015925 RA
      tatttttag caaagcgca cgcgtaacg tcttttaaa 
      ttcgccatt tttataatt tattgttgt gtgtttatt 
      aaaaaagca tgagatttt gttatgtaa atgagcagg 
      gaatcgaag cgatgattt cgtggggaa agtaatcac 
      gtggagtcg atgtaagat ggagcgccg ttgacaaat 
      cttacacaa ccaattata tacaaagaa tgggagcat 
      ttcttgaca agccaaaga cagataaat tccaagagt 
      gtggagaag gcaacgggc tgcgctatg gggtcgcca 
      gcatgcagg gctggaggg tcgagatgg aggacgcac 
      acatggcca agacgaacc tgggggacg ccctcaaag 
      actggtctt acttcgccg ttttcgatg gccatgctg 
      gggccaagg tgtcagccc actgcgccg agcacctcc 
      tcgacgcca tcatgcaaa cggaggagt tccaaaagg 
      acgtgatga aaggcatcc acaacggtt ttctagagt 
      tagataata aaatgcgct cgttacctg aaatgacgt 
      ccggcgaag acaagagcg gcacaacgg ccgtctgtg 
      cattcgttt cacctagac ttatctatg tagcgaatt 
      gcggggatt cccgcgccg ttttatgtc gcggtggca 
      gccccgttt tcaccacac aagaccaca agcccgggt 
      tgccttcgg aaagggagc gcattgtta aagcaggag 
      gcaatgtta tgatccaga gagtgaacg gaagtttgg 
      ccgtatcga gggctctag gcgattacg agtacaaaa 
      atgtcgaag gaagggggc cctgtgagc agttggttt 
      cgcccgaac cggaaatct tcgtcaggg atagagatg 
      ataaagagg atgagtttc tggtgttgg cttgcgatg 
      gaatttggg acgtgatga gcaacgagg atctctgcc 
      agtatatac ataatcgcc ttttagtca ccgataatt 
      tacaggaag ttaccagtc aagttatcg atacgtgtt 
      tgaacaaag gtagccgcg ataacatga gtattgtgt 
      tagttgtgt ttcctggcg ctccgaccc ccactcagg 
      aagcaatcg atgccgaaa aggaattcg atgcgactc 
      tggaaaggc gtataaaag aaattgtgg aacaaaacg 
      aaatcactg aattctcca cggtgctgc ataagcttg 
      catcactca acattgaag ggtttcctc caggtggtg 
      gactagctg caaaacgta cactgattg agaaaattt 
      tcaaagaac tgtgtccgg atctagctg aaactgatt 
      tggaccctt tcaactaaa tttggagcg cactttcag 
      gtgttgagg ttaatttta ggctattgc tgttttatt 
      tatttaaaa ggataagct tgattggca taggaattc 
      gtaatatta aaataatta taattttat tgcagccct 
      tgtactttt attcattaa caacaacac gaacggttt 
      tgtaattgc ttgttattc ctctaaaac tagggccta 
      acatttctt ttattagtt accaatata ctgcaaaag 
      tgatcctgt tttttgtat atttcgtaa gtagtgaag 
      ttttgtatg ttatgcaca aagctcatg tttcatcag 
      cgtttttcc ttataggat aataatgta aaatttgta 
      attactatt ttaaactag aggtgccac gttgcgtac 
      gtttagcta ttcaagtgt atgtggcca tcctctcta 
      ccaatttta aaaataata aatgtttgt agttgtttg 
      ttttcttta cttttcgcg atctgtgca aacatttat 
      tagcaacgg tacttctca tcatatcat tgcgttatc 
      tctgtacag ccattcaag aaattcgtt gaaattcta 
      ttttttaac tacctatat catgtgtat aaatcagaa 
      tgtgtgctg tatatttca gataattga gaatactct 
      aataattat gattaaatt atttaagta tca