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Gene information
General information
TC009608 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3224156..3228409
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009608
    iBeetle screen iB_02837

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009608 RA
      MSDESHPKT LYVGNLDPS VSEDLLCTL FSQIGPVKG 
      CKIIREPGN DPYAFVEFT NHQSASTAL AAMNKRLFL 
      DKEMKVNWA TSPGNQPKQ DTSNHHHIF VGDLSPEIE 
      TETLREAFA PFGEISNCR IVRDPQTLK SKGYAFVSF 
      VKKAEAENA IQAMNGQWL GSRSIRTNW STRKPPPPK 
      TEKAAQRAK QPTFDEVYN QSSPTNCTV YCGGFTTGL 
      TEDLMQKTF SQFGVIQDI RVFKDKGYA FIKFATKES 
      ATHAIETIH NTEINGQMV KCFWGKENG GMGADPGAA 
      MTSGGAAVA PGAQAQPYS YGYGGYWYQ GYGGYPYPY 
      QQYQYQQQY CMMPQQGQW ASAQPGGGQ QPNVPVMYK 
      YPSQ
    CDS sequence
    • >TC009608 RA
      atgtcggac gagagccat ccaaaaact ttgtatgtg 
      ggcaatctg gacccctcc gtcagcgag gacctgctc 
      tgcaccctc ttctcccag atcggcccc gtcaagggg 
      tgcaagatc atccgcgag cccggcaac gacccctac 
      gccttcgtc gagttcacc aaccaccag tcggcctcc 
      acggcgctg gcggcgatg aacaagcgc ctcttcctc 
      gacaaggag atgaaggtg aactgggcc acctcgccg 
      gggaatcag cccaagcag gacacctcc aaccaccac 
      cacatcttc gttggtgac ctgtcgccg gagatcgag 
      actgaaacc ctacgagag gccttcgct cccttcggg 
      gagatctcc aactgccgc atcgtgcgc gacccccaa 
      acgctcaaa agcaagggg tatgcgttc gtgtcgttt 
      gtcaagaaa gcggaggct gagaacgcg attcaggcg 
      atgaatggg caatggttg gggtccagg tccatcagg 
      accaactgg tccacgagg aagccgccg ccgccgaag 
      acggagaag gcggcgcaa cgcgccaaa cagcccact 
      tttgacgag gtttacaac cagagttcc ccgaccaat 
      tgcacggtg tactgcggc ggcttcacc accggcctc 
      accgaagac ctcatgcag aagaccttc tctcagttc 
      ggcgtcatc caggacatc cgagtgttc aaggacaaa 
      ggctacgcc ttcatcaaa ttcgcgacc aaagaatca 
      gcaacgcac gccatcgaa acaatccac aacaccgaa 
      atcaacggt caaatggtc aagtgtttc tggggcaag 
      gagaacggg ggcatgggc gcggacccg ggcgccgcc 
      atgacgtcg ggcggggcg gcggtggcg ccgggggcg 
      caggcccag ccctactcg tacggctac gggggctac 
      tggtaccag ggctacggg ggctatccc tacccctac 
      cagcagtac cagtaccag cagcagtac tgcatgatg 
      ccccagcag ggccagtgg gcctccgcc cagccgggg 
      ggcggccag cagcccaac gtgccggtg atgtacaag 
      tacccgtca cagtga
    mRNA sequence
    • >TC009608 RA
      acaaaaaaa attcattaa acagtttat taaaaaact 
      gatcctggt gtttcaaat tgcgcgtag taattttct 
      gcaccattt gaagatcga aacgagata aattggcgt 
      ggttttgcg gccgattga ccgtccgcg ctctatgta 
      agtgcttca gtggtgtgg gatttgggg acatttgct 
      tcttggccg ttttcgaat gacagctgt aagtgtctg 
      tagaaaagt tggccatca gtttttgtc ccttgttcg 
      caatcgttt cggtcgcga agccccctt atgttgtgt 
      tcgagggcg tagcctccc agccccgct gtatgtgca 
      atcggcttg tgtagtgtg gagtagtgc cgcccccag 
      cccgtgccg gcgtcacca tgtcggacg agagccatc 
      caaaaactt tgtatgtgg gcaatctgg acccctccg 
      tcagcgagg acctgctct gcaccctct tctcccaga 
      tcggccccg tcaaggggt gcaagatca tccgcgagc 
      ccggcaacg acccctacg ccttcgtcg agttcacca 
      accaccagt cggcctcca cggcgctgg cggcgatga 
      acaagcgcc tcttcctcg acaaggaga tgaaggtga 
      actgggcca cctcgccgg ggaatcagc ccaagcagg 
      acacctcca accaccacc acatcttcg ttggtgacc 
      tgtcgccgg agatcgaga ctgaaaccc tacgagagg 
      ccttcgctc ccttcgggg agatctcca actgccgca 
      tcgtgcgcg acccccaaa cgctcaaaa gcaaggggt 
      atgcgttcg tgtcgtttg tcaagaaag cggaggctg 
      agaacgcga ttcaggcga tgaatgggc aatggttgg 
      ggtccaggt ccatcagga ccaactggt ccacgagga 
      agccgccgc cgccgaaga cggagaagg cggcgcaac 
      gcgccaaac agcccactt ttgacgagg tttacaacc 
      agagttccc cgaccaatt gcacggtgt actgcggcg 
      gcttcacca ccggcctca ccgaagacc tcatgcaga 
      agaccttct ctcagttcg gcgtcatcc aggacatcc 
      gagtgttca aggacaaag gctacgcct tcatcaaat 
      tcgcgacca aagaatcag caacgcacg ccatcgaaa 
      caatccaca acaccgaaa tcaacggtc aaatggtca 
      agtgtttct ggggcaagg agaacgggg gcatgggcg 
      cggacccgg gcgccgcca tgacgtcgg gcggggcgg 
      cggtggcgc cgggggcgc aggcccagc cctactcgt 
      acggctacg ggggctact ggtaccagg gctacgggg 
      gctatccct acccctacc agcagtacc agtaccagc 
      agcagtact gcatgatgc cccagcagg gccagtggg 
      cctccgccc agccggggg gcggccagc agcccaacg 
      tgccggtga tgtacaagt acccgtcac agtgatgag 
      gacgaaacc gtggcggtc tgacgtgca gtgacttgg 
      ttttagtta agagactcg gtgattaca tatttggat 
      atgtagttg tcatcagcg cactttggt tggttcttc 
      agacgtggg tgtcgcgcc gctcataga attattttt 
      tcatactac atatttttg tatagttgc gtttcgatg 
      cgttttgtc tcttttgta tatagtagt tgatgtcaa 
      agtgcgatt ggtgcgctc gctcgctcg ctcgctctt 
      aaactgatc atatgcgtg atacaattt agttgtctt 
      ttttgtggc aaggagtga ctgggactg gacttcggc 
      ggtcgtgac aattaaaac agttactgt agatgatta 
      tgtataatt aggttagac aaacacatt gtaatcatt 
      aatatatca cgtttatct gagtaaatg attttatga 
      tgatacgca tgattggtc tttccaact gaataagac 
      agattttat aacaaaaaa ataattatg tatatgcta 
      gttgttaat aaaaacttc aggtctgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY