Status
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Gene information
General information
TC033589
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030223,TC030224
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1089256..1095093
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033589
    iBeetle screen iB_02835
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033589 RA
      MSRFLCLPF YSYFFYLSY IYTTYSRKL SGIFKYIDQ 
      MAGYTGFLA MLTSMVMFY KRSNDLKTL LSNLESIQI 
      YSIKPKERN SNHWVRSGL FALITGNVL FYPFLPSDV 
      SYNLFSFVP LVVNALDHL FLNDILSDI CDKFEQINQ 
      HFRRQIKSV DLFVIFPLT KAEKVRNLK EDEVTFSVQ 
      KIQELSHLH YKLANFTVK ISGLFEITT ITAMVMWFG 
      YVIDTMYLF IHIRSRQED TDTLVVIYT FNLFYLCFC 
      FYWLLVMVA MFSRTQQSA NKTATFVHE IWNKYALKN 
      EVDKRVRHL QLVAIRLLN TKLQFTAKD FFNLDWTFC 
      HMMIAALTT VVLSLGSFI VSVYLTTKI DNDGESISL 
      ASGWLDLYL GITIQTLGI ISNCINAKQ ITIFFDKIQ 
      RIDKQFGLL GYSINYRRI SIFVNLAIL GVFGEFVLV 
      FVPDYVFFV ENEPILYLI FSYCPIVTT GIIKIQFAT 
      CAHLILQRV NHVKKILEK ETQTVAFPK DNHFLALLD 
      IVHGVHNEL CNLCQVANS IYGFQNFLF VLSTFSIYG 
      IEEMRRDHG GDLVSASFD IVAGFLFEE ICICFDKNT 
      NINFLQHLL VRFVSNNIA IKLFNITTV EVQDKYFAF 
      LNYQVTNHL GANTIFFSS HKFYEHVLL EINERDFIR 
      RNLIYIFNW GRRPFSRYF VRNIINVMK VFVITNPRN 
      DTFRIFYNQ AVPYKKHHL EMVNWWQHG VGLFNHPTL 
      PAKYNNVFK DFKENVFKI PVIHKPPWH FVQYGNDSI 
      KVTGGRDDR ILSLLSKKL NFRYDYFDP PERIQGSSA 
      SENGTFKGV LGLIWKRQA EFFIGDVAL SHERANYVE 
      FSFITLADS GAFITHAPS KLNEALALL RPFQWQVWP 
      AIGVTFVVV GPVLYAIIA LPNAWRPRF RVRSHARLF 
      FDCTWFTTT VLLKQTGKE PSSSHKARF FIIILSISS 
      TYVINDMYS ANLTSLLAK PGREKAINN LNQLEKAMA 
      TRGYDLYVE RHSSSYSLF ENGTGIYSR LWQMMNRRQ 
      THFLLESVE EGVQLVRDS TNKAVIAGR ETLFFDIQR 
      FGASNFHLS EKLNTAYSA IALQLGCPY IEEINKILM 
      AIFEAGIIT KMTENEYEQ LGKKKQTTS ETEKELIPG 
      VKKENRRVA KVSEDNEKL QPISIKMLQ GTFYLLCIG 
      NIFSGFILL AEILVYKHR KTYKHKKRR HRFVYLRKI 
      RHSVASKFG AVVDAVRRV YRRAMHDAF VATLEYLE
    CDS sequence
    • >TC033589 RA
      atgtcccgt ttcttgtgc ctccctttc tactcctac 
      tttttctac ctctcctac atctacaca acctactcc 
      cgaaaactc tccggaatt ttcaaatac atcgaccaa 
      atggccggt tacaccggc ttcctggcc atgttaacg 
      tccatggta atgttttac aaacgcagc aacgactta 
      aaaaccctc ctatcaaac ctcgaatcg atccaaatc 
      tactcaatc aagcccaaa gaacgcaac tcgaaccat 
      tgggtgcga agtggccta tttgcctta atcaccggc 
      aatgtctta ttttacccg tttctgcca agcgatgtg 
      agctacaac ttgttcagt ttcgtccct ctggtggtc 
      aacgctttg gaccacctc ttcctcaac gacattctc 
      agcgacatt tgcgacaaa ttcgagcaa atcaaccag 
      cacttccgg cgccagatc aaatccgtt gatctgttc 
      gtcattttc ccgctcacc aaagccgaa aaagtgaga 
      aacttgaaa gaggacgaa gtcacgttc agcgtgcaa 
      aaaatccaa gaactgtcg cacttgcac tacaaactc 
      gccaatttc acggtgaaa atcagcgga ttgtttgaa 
      atcaccaca atcacggcc atggtgatg tggttcggt 
      tatgtgatc gatacgatg tatctcttc attcacatc 
      aggtcgaga caggaagac accgacact cttgtagtc 
      atctacacc ttcaacttg ttttactta tgtttctgc 
      ttctattgg ctgctcgtg atggtggca atgtttagc 
      cgaacgcaa caaagcgcc aacaaaacg gccactttt 
      gtccacgaa atttggaac aagtacgcc ctcaaaaac 
      gaggtggac aaaagggtg cgacacttg cagttggtc 
      gcaattagg ctcctcaat accaaactg caattcact 
      gcgaaggat tttttcaat ttggactgg acgttctgt 
      cacatgatg attgcggcc ctgaccacc gttgtttta 
      tcgctgggt tcgttcatc gtttccgtt tatttgacg 
      acaaagatt gacaatgat ggagaatcg atttctctg 
      gccagtggt tggttggac ttgtatttg gggatcacc 
      atacaaacc ctcggaatt atctccaat tgcataaac 
      gcgaaacaa attacgatt ttctttgac aaaatccaa 
      cgaatcgat aagcaattc ggtctcctg ggctattca 
      attaattac aggagaatt tcaattttc gtcaatttg 
      gccattctt ggcgttttc ggcgaattt gtgctagtt 
      tttgtccca gattatgtt tttttcgta gaaaatgaa 
      ccgattctc tacttgata ttctcctat tgtcccatc 
      gtgaccaca gggattatt aaaatccag tttgcgacg 
      tgtgcgcac ctgattttg caacgagta aatcatgtg 
      aagaaaatt ttggaaaaa gaaacacaa actgtggct 
      tttcccaaa gataatcat tttttggct cttctggac 
      attgtccat ggcgttcac aacgaactg tgcaatttg 
      tgccaagtt gcaaattcg atatacggt ttccagaat 
      tttttattc gtcttgtcc acattttcc atctatgga 
      atcgaagaa atgcgacga gaccacgga ggcgacctt 
      gtcagtgcc tcctttgac atcgtcgcc ggtttcctc 
      tttgaagaa atttgcata tgtttcgac aaaaacaca 
      aatattaac tttttgcag cacctttta gtccgtttc 
      gtgagcaac aacatcgcc atcaaacta ttcaatatc 
      acaacggtt gaagtccaa gacaaatat ttcgcgttt 
      ttgaactac caagtcacc aatcatctc ggagcgaat 
      acaatattt ttctcttcg cacaaattt tacgagcac 
      gttttactc gagataaat gaacgcgat ttcatccgc 
      cgtaacttg atctatatt ttcaactgg ggccggcgg 
      ccctttagc cgctacttc gtccgcaat ataataaat 
      gtgatgaaa gttttcgtc atcacaaac ccgcgaaac 
      gacacgttt cgcattttc tacaaccaa gcggttcca 
      tataaaaaa caccacttg gaaatggtc aactggtgg 
      caacacggg gttggcctc ttcaaccac ccgacactt 
      cccgccaaa tacaataac gtattcaaa gactttaaa 
      gaaaacgtt tttaaaata ccagtgatt cataaacca 
      ccctggcac ttcgtccag tacggtaac gactcgatt 
      aaagtaaca ggagggcgc gacgaccgc attttatcg 
      ctcttgtcc aaaaagctc aactttcgg tacgactac 
      ttcgacccc ccggagcgc atccaaggc tcgtcggcg 
      tcggaaaac ggcactttc aagggggtt ttgggcctg 
      atttggaaa cgacaggca gaatttttc atcggcgat 
      gtggcccta agccacgaa cgcgccaat tacgtcgaa 
      ttctccttc atcacttta gccgacagt ggcgctttt 
      atcacccat gcccccagt aaactgaac gaggctttg 
      gcccttctt aggccgttc cagtggcag gtttggccc 
      gccattggg gtgactttt gtggtcgtg gggccggta 
      ctttacgcg attatcgca ctgcctaat gcttggagg 
      cccaggttt cgggtcagg tcacatgcc cgacttttc 
      ttcgactgt acttggttc actaccact gttctcttg 
      aagcaaacg gggaaagag cccagttcg agtcataaa 
      gccaggttt ttcattatt attctctcg atttcgtcc 
      acgtatgtg attaacgac atgtattcg gctaacttg 
      acttcgctc cttgccaaa cctggacga gagaaggca 
      attaataat ttgaatcag ctggagaaa gcaatggcc 
      actcgaggc tatgattta tacgttgag aggcacagc 
      tcgtcctat agtttattt gagaacggg actgggatt 
      tacagccga ttgtggcaa atgatgaat cggcgccaa 
      acacacttt cttctcgaa tcggtcgaa gaaggggtc 
      caacttgtc cgagacagt accaacaag gctgttata 
      gcaggccgt gagaccctc ttcttcgac atacaaaga 
      tttggtgcc agcaatttc catctgagt gagaaacta 
      aacactgca tattcagca attgcccta cagctcggt 
      tgtccttac atcgaagaa ataaataaa atccttatg 
      gccattttc gaggccggc attataaca aaaatgacc 
      gaaaatgag tacgaacaa ttgggcaaa aagaaacaa 
      acaacttcc gaaacagaa aaagagtta atacccgga 
      gtgaagaaa gaaaatcgc agagtggcc aaagtcagt 
      gaagataac gagaaactc caacccatc agcattaaa 
      atgctgcaa gggactttc tatttattg tgcatcgga 
      aatatattt tcaggtttt attctactt gcagaaatt 
      ctggtttac aagcatcgg aaaacgtac aaacacaaa 
      aaacggaga cataggttc gtttatttg aggaaaatt 
      agacacagt gttgcctcg aaatttgga gcagttgtc 
      gatgctgtg aggagggtt tacagaaga gcaatgcac 
      gatgcgttt gtcgccaca ttggagtat ttggaataa
    mRNA sequence
    • >TC033589 RA
      cagcgccca cttttcctt catgtcccg tttcttgtg 
      cctcccttt ctactccta ctttttcta cctctccta 
      catctacac aacctactc ccgaaaact ctccggaat 
      tttcaaata catcgacca aatggccgg ttacaccgg 
      cttcctggc catgttaac gtccatggt aatgtttta 
      caaacgcag caacgactt aaaaaccct cctatcaaa 
      cctcgaatc gatccaaat ctactcaat caagcccaa 
      agaacgcaa ctcgaacca ttgggtgcg aagtggcct 
      atttgcctt aatcaccgg caatgtctt attttaccc 
      gtttctgcc aagcgatgt gagctacaa cttgttcag 
      tttcgtccc tctggtggt caacgcttt ggaccacct 
      cttcctcaa cgacattct cagcgacat ttgcgacaa 
      attcgagca aatcaacca gcacttccg gcgccagat 
      caaatccgt tgatctgtt cgtcatttt cccgctcac 
      caaagccga aaaagtgag aaacttgaa agaggacga 
      agtcacgtt cagcgtgca aaaaatcca agaactgtc 
      gcacttgca ctacaaact cgccaattt cacggtgaa 
      aatcagcgg attgtttga aatcaccac aatcacggc 
      catggtgat gtggttcgg ttatgtgat cgatacgat 
      gtatctctt cattcacat caggtcgag acaggaaga 
      caccgacac tcttgtagt catctacac cttcaactt 
      gttttactt atgtttctg cttctattg gctgctcgt 
      gatggtggc aatgtttag ccgaacgca acaaagcgc 
      caacaaaac ggccacttt tgtccacga aatttggaa 
      caagtacgc cctcaaaaa cgaggtgga caaaagggt 
      gcgacactt gcagttggt cgcaattag gctcctcaa 
      taccaaact gcaattcac tgcgaagga ttttttcaa 
      tttggactg gacgttctg tcacatgat gattgcggc 
      cctgaccac cgttgtttt atcgctggg ttcgttcat 
      cgtttccgt ttatttgac gacaaagat tgacaatga 
      tggagaatc gatttctct ggccagtgg ttggttgga 
      cttgtattt ggggatcac catacaaac cctcggaat 
      tatctccaa ttgcataaa cgcgaaaca aattacgat 
      tttctttga caaaatcca acgaatcga taagcaatt 
      cggtctcct gggctattc aattaatta caggagaat 
      ttcaatttt cgtcaattt ggccattct tggcgtttt 
      cggcgaatt tgtgctagt ttttgtccc agattatgt 
      ttttttcgt agaaaatga accgattct ctacttgat 
      attctccta ttgtcccat cgtgaccac agggattat 
      taaaatcca gtttgcgac gtgtgcgca cctgatttt 
      gcaacgagt aaatcatgt gaagaaaat tttggaaaa 
      agaaacaca aactgtggc ttttcccaa agataatca 
      ttttttggc tcttctgga cattgtcca tggcgttca 
      caacgaact gtgcaattt gtgccaagt tgcaaattc 
      gatatacgg tttccagaa ttttttatt cgtcttgtc 
      cacattttc catctatgg aatcgaaga aatgcgacg 
      agaccacgg aggcgacct tgtcagtgc ctcctttga 
      catcgtcgc cggtttcct ctttgaaga aatttgcat 
      atgtttcga caaaaacac aaatattaa ctttttgca 
      gcacctttt agtccgttt cgtgagcaa caacatcgc 
      catcaaact attcaatat cacaacggt tgaagtcca 
      agacaaata tttcgcgtt tttgaacta ccaagtcac 
      caatcatct cggagcgaa tacaatatt tttctcttc 
      gcacaaatt ttacgagca cgttttact cgagataaa 
      tgaacgcga tttcatccg ccgtaactt gatctatat 
      tttcaactg gggccggcg gccctttag ccgctactt 
      cgtccgcaa tataataaa tgtgatgaa agttttcgt 
      catcacaaa cccgcgaaa cgacacgtt tcgcatttt 
      ctacaacca agcggttcc atataaaaa acaccactt 
      ggaaatggt caactggtg gcaacacgg ggttggcct 
      cttcaacca cccgacact tcccgccaa atacaataa 
      cgtattcaa agactttaa agaaaacgt ttttaaaat 
      accagtgat tcataaacc accctggca cttcgtcca 
      gtacggtaa cgactcgat taaagtaac aggagggcg 
      cgacgaccg cattttatc gctcttgtc caaaaagct 
      caactttcg gtacgacta cttcgaccc cccggagcg 
      catccaagg ctcgtcggc gtcggaaaa cggcacttt 
      caagggggt tttgggcct gatttggaa acgacaggc 
      agaattttt catcggcga tgtggccct aagccacga 
      acgcgccaa ttacgtcga attctcctt catcacttt 
      agccgacag tggcgcttt tatcaccca tgcccccag 
      taaactgaa cgaggcttt ggcccttct taggccgtt 
      ccagtggca ggtttggcc cgccattgg ggtgacttt 
      tgtggtcgt ggggccggt actttacgc gattatcgc 
      actgcctaa tgcttggag gcccaggtt tcgggtcag 
      gtcacatgc ccgactttt cttcgactg tacttggtt 
      cactaccac tgttctctt gaagcaaac ggggaaaga 
      gcccagttc gagtcataa agccaggtt tttcattat 
      tattctctc gatttcgtc cacgtatgt gattaacga 
      catgtattc ggctaactt gacttcgct ccttgccaa 
      acctggacg agagaaggc aattaataa tttgaatca 
      gctggagaa agcaatggc cactcgagg ctatgattt 
      atacgttga gaggcacag ctcgtccta tagtttatt 
      tgagaacgg gactgggat ttacagccg attgtggca 
      aatgatgaa tcggcgcca aacacactt tcttctcga 
      atcggtcga agaaggggt ccaacttgt ccgagacag 
      taccaacaa ggctgttat agcaggccg tgagaccct 
      cttcttcga catacaaag atttggtgc cagcaattt 
      ccatctgag tgagaaact aaacactgc atattcagc 
      aattgccct acagctcgg ttgtcctta catcgaaga 
      aataaataa aatccttat ggccatttt cgaggccgg 
      cattataac aaaaatgac cgaaaatga gtacgaaca 
      attgggcaa aaagaaaca aacaacttc cgaaacaga 
      aaaagagtt aatacccgg agtgaagaa agaaaatcg 
      cagagtggc caaagtcag tgaagataa cgagaaact 
      ccaacccat cagcattaa aatgctgca agggacttt 
      ctatttatt gtgcatcgg aaatatatt ttcaggttt 
      tattctact tgcagaaat tctggttta caagcatcg 
      gaaaacgta caaacacaa aaaacggag acataggtt 
      cgtttattt gaggaaaat tagacacag tgttgcctc 
      gaaatttgg agcagttgt cgatgctgt gaggagggt 
      ttacagaag agcaatgca cgatgcgtt tgtcgccac 
      attggagta tttggaata atttttaga tatgtagtt 
      tatcacaaa aacaaacaa acaaaaaca ggaaaacgc 
      tgtagctta tagctagta aataataca aataaattg 
      gaaaaactg ta