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Gene information
General information
TC009598 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3086343..3089968
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009598
    iBeetle screen iB_02834

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009598 RA
      MLESLRNRV FVIYLSVSV YYVIIPIGF VTNWWLSVD 
      VMAPNGTIL VTGGAGYVG SHTVVELLN NNYSVIAID 
      NLVNCYAEK NQKPESLKR VEQITKKQI TFYNVDIRN 
      KEALDKIFK THKIDAVIH FAALKAVGE SVQVPLMYY 
      QNNIAGSST LFEVMQNNG VKKVVFSSS ATVYGLPQH 
      LPITEAHPT GQNCTNPYG KTKFFVEEI LKDVCTSDP 
      EWKVILLRY FNPVGAHSS GLIGEDPSG IPNNLMPYI 
      SQVAIGRRE KVFVFGNDY STKDGTGVR DYIHITDLA 
      IGHLKALEK MNEADFKGW KAYNLGTGQ GYSVLDLIN 
      AFAKASGQK INYEVIKRR EGDIASSFA DPSLAKKEL 
      NWTATRGID EMCRDTWNW QKLNPNGFQ KK
    • >TC009598 RB
      MAPNGTILV TGGAGYVGS HTVVELLNN NYSVIAIDN 
      LVNCYAEKN QKPESLKRV EQITKKQIT FYNVDIRNK 
      EALDKIFKT HKIDAVIHF AALKAVGES VQVPLMYYQ 
      NNIAGSSTL FEVMQNNGV KKVVFSSSA TVYGLPQHL 
      PITEAHPTG QNCTNPYGK TKFFVEEIL KDVCTSDPE 
      WKVILLRYF NPVGAHSSG LIGEDPSGI PNNLMPYIS 
      QVAIGRREK VFVFGNDYS TKDGTGVRD YIHITDLAI 
      GHLKALEKM NEADFKGWK AYNLGTGQG YSVLDLINA 
      FAKASGQKI NYEVIKRRE GDIASSFAD PSLAKKELN 
      WTATRGIDE MCRDTWNWQ KLNPNGFQK K
    CDS sequence
    • >TC009598 RA
      atgctcgaa tcattgcga aaccgcgtg tttgttatt 
      tatttaagc gtgtctgtg tattacgtg ataattccg 
      atcggcttc gtgacaaat tggtggcta tcagtggac 
      gtaatggct ccaaacggc actattctt gtaacaggg 
      ggcgccggt tacgtaggc tctcacacc gttgtggaa 
      ctactaaat aataattat tcagtaatt gccatcgac 
      aatctcgtc aattgttac gccgaaaaa aaccaaaaa 
      ccggagtcc ctcaaacgt gtcgaacaa atcacaaag 
      aaacaaatc actttttac aatgttgac atccgaaac 
      aaagaagct ttggacaag attttcaaa actcataaa 
      atcgatgcc gttatccac tttgccgcc cttaaggcg 
      gttggcgag tcagtccag gtgccccta atgtactac 
      cagaacaac atagccggc tcgagcact ttatttgaa 
      gtgatgcaa aataacggg gtgaaaaaa gttgttttt 
      tcgtcgtct gctacagtt tacgggtta ccccaacat 
      ttaccaatc acagaagcg catccgacc ggacaaaat 
      tgtacaaat ccgtacgga aaaacgaaa tttttcgtt 
      gaagaaatt ctgaaagat gtgtgcacg tcagatcct 
      gaatggaaa gtcatttta ttgcgatac tttaacccc 
      gtaggggca cacagctca ggtctcatt ggcgaggac 
      ccctcagga atccccaac aatttaatg ccatatatt 
      tctcaggta gctataggt cgtagggag aaagtgttt 
      gtttttggc aacgactac tcaaccaaa gacggtacg 
      ggagtccgt gactacatc catatcacg gacttggca 
      attgggcat ttgaaggcg cttgaaaag atgaatgag 
      gccgatttc aagggttgg aaggcctac aacttgggc 
      acgggccaa ggttattca gtattagat ttgatcaat 
      gcttttgcg aaagcctct ggtcaaaaa atcaactat 
      gaagtgatc aaacgaaga gaaggggat attgcgagc 
      agttttgcg gacccttct ctggccaaa aaagaactg 
      aactggacg gcaacgcga ggcattgat gagatgtgt 
      cgggacacg tggaattgg caaaaactg aacccgaac 
      gggtttcag aagaaatga
    • >TC009598 RB
      atggctcca aacggcact attcttgta acagggggc 
      gccggttac gtaggctct cacaccgtt gtggaacta 
      ctaaataat aattattca gtaattgcc atcgacaat 
      ctcgtcaat tgttacgcc gaaaaaaac caaaaaccg 
      gagtccctc aaacgtgtc gaacaaatc acaaagaaa 
      caaatcact ttttacaat gttgacatc cgaaacaaa 
      gaagctttg gacaagatt ttcaaaact cataaaatc 
      gatgccgtt atccacttt gccgccctt aaggcggtt 
      ggcgagtca gtccaggtg cccctaatg tactaccag 
      aacaacata gccggctcg agcacttta tttgaagtg 
      atgcaaaat aacggggtg aaaaaagtt gttttttcg 
      tcgtctgct acagtttac gggttaccc caacattta 
      ccaatcaca gaagcgcat ccgaccgga caaaattgt 
      acaaatccg tacggaaaa acgaaattt ttcgttgaa 
      gaaattctg aaagatgtg tgcacgtca gatcctgaa 
      tggaaagtc attttattg cgatacttt aaccccgta 
      ggggcacac agctcaggt ctcattggc gaggacccc 
      tcaggaatc cccaacaat ttaatgcca tatatttct 
      caggtagct ataggtcgt agggagaaa gtgtttgtt 
      tttggcaac gactactca accaaagac ggtacggga 
      gtccgtgac tacatccat atcacggac ttggcaatt 
      gggcatttg aaggcgctt gaaaagatg aatgaggcc 
      gatttcaag ggttggaag gcctacaac ttgggcacg 
      ggccaaggt tattcagta ttagatttg atcaatgct 
      tttgcgaaa gcctctggt caaaaaatc aactatgaa 
      gtgatcaaa cgaagagaa ggggatatt gcgagcagt 
      tttgcggac ccttctctg gccaaaaaa gaactgaac 
      tggacggca acgcgaggc attgatgag atgtgtcgg 
      gacacgtgg aattggcaa aaactgaac ccgaacggg 
      tttcagaag aaatga
    mRNA sequence
    • >TC009598 RA
      tgggacgtc cgctgtcaa cgtcacatc ctaaataca 
      aaaattttt aaacctctc aatagtgat aagaagtga 
      taacaatgc tcgaatcat tgcgaaacc gcgtgtttg 
      ttatttatt taagcgtgt ctgtgtatt acgtgataa 
      ttccgatcg gcttcgtga caaattggt ggctatcag 
      tggacgtaa tggctccaa acggcacta ttcttgtaa 
      cagggggcg ccggttacg taggctctc acaccgttg 
      tggaactac taaataata attattcag taattgcca 
      tcgacaatc tcgtcaatt gttacgccg aaaaaaacc 
      aaaaaccgg agtccctca aacgtgtcg aacaaatca 
      caaagaaac aaatcactt tttacaatg ttgacatcc 
      gaaacaaag aagctttgg acaagattt tcaaaactc 
      ataaaatcg atgccgtta tccactttg ccgccctta 
      aggcggttg gcgagtcag tccaggtgc ccctaatgt 
      actaccaga acaacatag ccggctcga gcactttat 
      ttgaagtga tgcaaaata acggggtga aaaaagttg 
      ttttttcgt cgtctgcta cagtttacg ggttacccc 
      aacatttac caatcacag aagcgcatc cgaccggac 
      aaaattgta caaatccgt acggaaaaa cgaaatttt 
      tcgttgaag aaattctga aagatgtgt gcacgtcag 
      atcctgaat ggaaagtca ttttattgc gatacttta 
      accccgtag gggcacaca gctcaggtc tcattggcg 
      aggacccct caggaatcc ccaacaatt taatgccat 
      atatttctc aggtagcta taggtcgta gggagaaag 
      tgtttgttt ttggcaacg actactcaa ccaaagacg 
      gtacgggag tccgtgact acatccata tcacggact 
      tggcaattg ggcatttga aggcgcttg aaaagatga 
      atgaggccg atttcaagg gttggaagg cctacaact 
      tgggcacgg gccaaggtt attcagtat tagatttga 
      tcaatgctt ttgcgaaag cctctggtc aaaaaatca 
      actatgaag tgatcaaac gaagagaag gggatattg 
      cgagcagtt ttgcggacc cttctctgg ccaaaaaag 
      aactgaact ggacggcaa cgcgaggca ttgatgaga 
      tgtgtcggg acacgtgga attggcaaa aactgaacc 
      cgaacgggt ttcagaaga aatgatgca tttatgacg 
      tatttagat ttttgttga gcaatgact ggtacttaa 
      gcatttata cacccagta atttttgcc atattttat 
      tctattgca tgttgaaaa taaaactat ttttctaat
    • >TC009598 RB
      aatacaaaa atttttaaa cctctcaat agtgataag 
      aagtgataa caatgctcg aatcattgc gaaaccgcg 
      tgtttgtta tttatttaa gcgtgtctg tgtattacg 
      tgataattc cgatcggct tcgtgacaa agtaagttg 
      gtggctatc agtggacgt aatggctcc aaacggcac 
      tattcttgt aacaggggg cgccggtta cgtaggctc 
      tcacaccgt tgtggaact actaaataa taattattc 
      agtaattgc catcgacaa tctcgtcaa ttgttacgc 
      cgaaaaaaa ccaaaaacc ggagtccct caaacgtgt 
      cgaacaaat cacaaagaa acaaatcac tttttacaa 
      tgttgacat ccgaaacaa agaagcttt ggacaagat 
      tttcaaaac tcataaaat cgatgccgt tatccactt 
      tgccgccct taaggcggt tggcgagtc agtccaggt 
      gcccctaat gtactacca gaacaacat agccggctc 
      gagcacttt atttgaagt gatgcaaaa taacggggt 
      gaaaaaagt tgttttttc gtcgtctgc tacagttta 
      cgggttacc ccaacattt accaatcac agaagcgca 
      tccgaccgg acaaaattg tacaaatcc gtacggaaa 
      aacgaaatt tttcgttga agaaattct gaaagatgt 
      gtgcacgtc agatcctga atggaaagt cattttatt 
      gcgatactt taaccccgt aggggcaca cagctcagg 
      tctcattgg cgaggaccc ctcaggaat ccccaacaa 
      tttaatgcc atatatttc tcaggtagc tataggtcg 
      tagggagaa agtgtttgt ttttggcaa cgactactc 
      aaccaaaga cggtacggg agtccgtga ctacatcca 
      tatcacgga cttggcaat tgggcattt gaaggcgct 
      tgaaaagat gaatgaggc cgatttcaa gggttggaa 
      ggcctacaa cttgggcac gggccaagg ttattcagt 
      attagattt gatcaatgc ttttgcgaa agcctctgg 
      tcaaaaaat caactatga agtgatcaa acgaagaga 
      aggggatat tgcgagcag ttttgcgga cccttctct 
      ggccaaaaa agaactgaa ctggacggc aacgcgagg 
      cattgatga gatgtgtcg ggacacgtg gaattggca 
      aaaactgaa cccgaacgg gtttcagaa gaaatgatg 
      catttatga cgtatttag atttttgtt gagcaatga 
      ctggtactt aagcattta tacacccag taatttttg 
      ccatatttt attctattg catgttgaa aataaaact 
      atttttcta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY