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Gene information
General information
TC009598 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3086343..3089968
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009598
    iBeetle screen iB_02834

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: >5 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009598 RA
      MLESLRNRV FVIYLSVSV YYVIIPIGF VTNWWLSVD 
VMAPNGTIL VTGGAGYVG SHTVVELLN NNYSVIAID 
NLVNCYAEK NQKPESLKR VEQITKKQI TFYNVDIRN 
KEALDKIFK THKIDAVIH FAALKAVGE SVQVPLMYY 
QNNIAGSST LFEVMQNNG VKKVVFSSS ATVYGLPQH 
LPITEAHPT GQNCTNPYG KTKFFVEEI LKDVCTSDP 
EWKVILLRY FNPVGAHSS GLIGEDPSG IPNNLMPYI 
SQVAIGRRE KVFVFGNDY STKDGTGVR DYIHITDLA 
IGHLKALEK MNEADFKGW KAYNLGTGQ GYSVLDLIN 
AFAKASGQK INYEVIKRR EGDIASSFA DPSLAKKEL 
NWTATRGID EMCRDTWNW QKLNPNGFQ KK
    • >TC009598 RB
      MAPNGTILV TGGAGYVGS HTVVELLNN NYSVIAIDN 
LVNCYAEKN QKPESLKRV EQITKKQIT FYNVDIRNK 
EALDKIFKT HKIDAVIHF AALKAVGES VQVPLMYYQ 
NNIAGSSTL FEVMQNNGV KKVVFSSSA TVYGLPQHL 
PITEAHPTG QNCTNPYGK TKFFVEEIL KDVCTSDPE 
WKVILLRYF NPVGAHSSG LIGEDPSGI PNNLMPYIS 
QVAIGRREK VFVFGNDYS TKDGTGVRD YIHITDLAI 
GHLKALEKM NEADFKGWK AYNLGTGQG YSVLDLINA 
FAKASGQKI NYEVIKRRE GDIASSFAD PSLAKKELN 
WTATRGIDE MCRDTWNWQ KLNPNGFQK K
    CDS sequence
    • >TC009598 RA
      atgctcgaa tcattgcga aaccgcgtg tttgttatt 
tatttaagc gtgtctgtg tattacgtg ataattccg 
atcggcttc gtgacaaat tggtggcta tcagtggac 
gtaatggct ccaaacggc actattctt gtaacaggg 
ggcgccggt tacgtaggc tctcacacc gttgtggaa 
ctactaaat aataattat tcagtaatt gccatcgac 
aatctcgtc aattgttac gccgaaaaa aaccaaaaa 
ccggagtcc ctcaaacgt gtcgaacaa atcacaaag 
aaacaaatc actttttac aatgttgac atccgaaac 
aaagaagct ttggacaag attttcaaa actcataaa 
atcgatgcc gttatccac tttgccgcc cttaaggcg 
gttggcgag tcagtccag gtgccccta atgtactac 
cagaacaac atagccggc tcgagcact ttatttgaa 
gtgatgcaa aataacggg gtgaaaaaa gttgttttt 
tcgtcgtct gctacagtt tacgggtta ccccaacat 
ttaccaatc acagaagcg catccgacc ggacaaaat 
tgtacaaat ccgtacgga aaaacgaaa tttttcgtt 
gaagaaatt ctgaaagat gtgtgcacg tcagatcct 
gaatggaaa gtcatttta ttgcgatac tttaacccc 
gtaggggca cacagctca ggtctcatt ggcgaggac 
ccctcagga atccccaac aatttaatg ccatatatt 
tctcaggta gctataggt cgtagggag aaagtgttt 
gtttttggc aacgactac tcaaccaaa gacggtacg 
ggagtccgt gactacatc catatcacg gacttggca 
attgggcat ttgaaggcg cttgaaaag atgaatgag 
gccgatttc aagggttgg aaggcctac aacttgggc 
acgggccaa ggttattca gtattagat ttgatcaat 
gcttttgcg aaagcctct ggtcaaaaa atcaactat 
gaagtgatc aaacgaaga gaaggggat attgcgagc 
agttttgcg gacccttct ctggccaaa aaagaactg 
aactggacg gcaacgcga ggcattgat gagatgtgt 
cgggacacg tggaattgg caaaaactg aacccgaac 
gggtttcag aagaaatga
    • >TC009598 RB
      atggctcca aacggcact attcttgta acagggggc 
gccggttac gtaggctct cacaccgtt gtggaacta 
ctaaataat aattattca gtaattgcc atcgacaat 
ctcgtcaat tgttacgcc gaaaaaaac caaaaaccg 
gagtccctc aaacgtgtc gaacaaatc acaaagaaa 
caaatcact ttttacaat gttgacatc cgaaacaaa 
gaagctttg gacaagatt ttcaaaact cataaaatc 
gatgccgtt atccacttt gccgccctt aaggcggtt 
ggcgagtca gtccaggtg cccctaatg tactaccag 
aacaacata gccggctcg agcacttta tttgaagtg 
atgcaaaat aacggggtg aaaaaagtt gttttttcg 
tcgtctgct acagtttac gggttaccc caacattta 
ccaatcaca gaagcgcat ccgaccgga caaaattgt 
acaaatccg tacggaaaa acgaaattt ttcgttgaa 
gaaattctg aaagatgtg tgcacgtca gatcctgaa 
tggaaagtc attttattg cgatacttt aaccccgta 
ggggcacac agctcaggt ctcattggc gaggacccc 
tcaggaatc cccaacaat ttaatgcca tatatttct 
caggtagct ataggtcgt agggagaaa gtgtttgtt 
tttggcaac gactactca accaaagac ggtacggga 
gtccgtgac tacatccat atcacggac ttggcaatt 
gggcatttg aaggcgctt gaaaagatg aatgaggcc 
gatttcaag ggttggaag gcctacaac ttgggcacg 
ggccaaggt tattcagta ttagatttg atcaatgct 
tttgcgaaa gcctctggt caaaaaatc aactatgaa 
gtgatcaaa cgaagagaa ggggatatt gcgagcagt 
tttgcggac ccttctctg gccaaaaaa gaactgaac 
tggacggca acgcgaggc attgatgag atgtgtcgg 
gacacgtgg aattggcaa aaactgaac ccgaacggg 
tttcagaag aaatga
    mRNA sequence
    • >TC009598 RA
      tgggacgtc cgctgtcaa cgtcacatc ctaaataca 
aaaattttt aaacctctc aatagtgat aagaagtga 
taacaatgc tcgaatcat tgcgaaacc gcgtgtttg 
ttatttatt taagcgtgt ctgtgtatt acgtgataa 
ttccgatcg gcttcgtga caaattggt ggctatcag 
tggacgtaa tggctccaa acggcacta ttcttgtaa 
cagggggcg ccggttacg taggctctc acaccgttg 
tggaactac taaataata attattcag taattgcca 
tcgacaatc tcgtcaatt gttacgccg aaaaaaacc 
aaaaaccgg agtccctca aacgtgtcg aacaaatca 
caaagaaac aaatcactt tttacaatg ttgacatcc 
gaaacaaag aagctttgg acaagattt tcaaaactc 
ataaaatcg atgccgtta tccactttg ccgccctta 
aggcggttg gcgagtcag tccaggtgc ccctaatgt 
actaccaga acaacatag ccggctcga gcactttat 
ttgaagtga tgcaaaata acggggtga aaaaagttg 
ttttttcgt cgtctgcta cagtttacg ggttacccc 
aacatttac caatcacag aagcgcatc cgaccggac 
aaaattgta caaatccgt acggaaaaa cgaaatttt 
tcgttgaag aaattctga aagatgtgt gcacgtcag 
atcctgaat ggaaagtca ttttattgc gatacttta 
accccgtag gggcacaca gctcaggtc tcattggcg 
aggacccct caggaatcc ccaacaatt taatgccat 
atatttctc aggtagcta taggtcgta gggagaaag 
tgtttgttt ttggcaacg actactcaa ccaaagacg 
gtacgggag tccgtgact acatccata tcacggact 
tggcaattg ggcatttga aggcgcttg aaaagatga 
atgaggccg atttcaagg gttggaagg cctacaact 
tgggcacgg gccaaggtt attcagtat tagatttga 
tcaatgctt ttgcgaaag cctctggtc aaaaaatca 
actatgaag tgatcaaac gaagagaag gggatattg 
cgagcagtt ttgcggacc cttctctgg ccaaaaaag 
aactgaact ggacggcaa cgcgaggca ttgatgaga 
tgtgtcggg acacgtgga attggcaaa aactgaacc 
cgaacgggt ttcagaaga aatgatgca tttatgacg 
tatttagat ttttgttga gcaatgact ggtacttaa 
gcatttata cacccagta atttttgcc atattttat 
tctattgca tgttgaaaa taaaactat ttttctaat
    • >TC009598 RB
      aatacaaaa atttttaaa cctctcaat agtgataag 
aagtgataa caatgctcg aatcattgc gaaaccgcg 
tgtttgtta tttatttaa gcgtgtctg tgtattacg 
tgataattc cgatcggct tcgtgacaa agtaagttg 
gtggctatc agtggacgt aatggctcc aaacggcac 
tattcttgt aacaggggg cgccggtta cgtaggctc 
tcacaccgt tgtggaact actaaataa taattattc 
agtaattgc catcgacaa tctcgtcaa ttgttacgc 
cgaaaaaaa ccaaaaacc ggagtccct caaacgtgt 
cgaacaaat cacaaagaa acaaatcac tttttacaa 
tgttgacat ccgaaacaa agaagcttt ggacaagat 
tttcaaaac tcataaaat cgatgccgt tatccactt 
tgccgccct taaggcggt tggcgagtc agtccaggt 
gcccctaat gtactacca gaacaacat agccggctc 
gagcacttt atttgaagt gatgcaaaa taacggggt 
gaaaaaagt tgttttttc gtcgtctgc tacagttta 
cgggttacc ccaacattt accaatcac agaagcgca 
tccgaccgg acaaaattg tacaaatcc gtacggaaa 
aacgaaatt tttcgttga agaaattct gaaagatgt 
gtgcacgtc agatcctga atggaaagt cattttatt 
gcgatactt taaccccgt aggggcaca cagctcagg 
tctcattgg cgaggaccc ctcaggaat ccccaacaa 
tttaatgcc atatatttc tcaggtagc tataggtcg 
tagggagaa agtgtttgt ttttggcaa cgactactc 
aaccaaaga cggtacggg agtccgtga ctacatcca 
tatcacgga cttggcaat tgggcattt gaaggcgct 
tgaaaagat gaatgaggc cgatttcaa gggttggaa 
ggcctacaa cttgggcac gggccaagg ttattcagt 
attagattt gatcaatgc ttttgcgaa agcctctgg 
tcaaaaaat caactatga agtgatcaa acgaagaga 
aggggatat tgcgagcag ttttgcgga cccttctct 
ggccaaaaa agaactgaa ctggacggc aacgcgagg 
cattgatga gatgtgtcg ggacacgtg gaattggca 
aaaactgaa cccgaacgg gtttcagaa gaaatgatg 
catttatga cgtatttag atttttgtt gagcaatga 
ctggtactt aagcattta tacacccag taatttttg 
ccatatttt attctattg catgttgaa aataaaact 
atttttcta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY