| General information | |||
| TC009598 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid47: 1004443..1008068 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009598 | |
| General information | ||||
| iB_02834 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC009598 RA
MLESLRNRV FVIYLSVSV YYVIIPIGF VTNWWLSVD VMAPNGTIL VTGGAGYVG SHTVVELLN NNYSVIAID NLVNCYAEK NQKPESLKR VEQITKKQI TFYNVDIRN KEALDKIFK THKIDAVIH FAALKAVGE SVQVPLMYY QNNIAGSST LFEVMQNNG VKKVVFSSS ATVYGLPQH LPITEAHPT GQNCTNPYG KTKFFVEEI LKDVCTSDP EWKVILLRY FNPVGAHSS GLIGEDPSG IPNNLMPYI SQVAIGRRE KVFVFGNDY STKDGTGVR DYIHITDLA IGHLKALEK MNEADFKGW KAYNLGTGQ GYSVLDLIN AFAKASGQK INYEVIKRR EGDIASSFA DPSLAKKEL NWTATRGID EMCRDTWNW QKLNPNGFQ KK
- >TC009598 RB
MAPNGTILV TGGAGYVGS HTVVELLNN NYSVIAIDN LVNCYAEKN QKPESLKRV EQITKKQIT FYNVDIRNK EALDKIFKT HKIDAVIHF AALKAVGES VQVPLMYYQ NNIAGSSTL FEVMQNNGV KKVVFSSSA TVYGLPQHL PITEAHPTG QNCTNPYGK TKFFVEEIL KDVCTSDPE WKVILLRYF NPVGAHSSG LIGEDPSGI PNNLMPYIS QVAIGRREK VFVFGNDYS TKDGTGVRD YIHITDLAI GHLKALEKM NEADFKGWK AYNLGTGQG YSVLDLINA FAKASGQKI NYEVIKRRE GDIASSFAD PSLAKKELN WTATRGIDE MCRDTWNWQ KLNPNGFQK K
- >TC009598 RA
atgctcgaa tcattgcga aaccgcgtg tttgttatt tatttaagc gtgtctgtg tattacgtg ataattccg atcggcttc gtgacaaat tggtggcta tcagtggac gtaatggct ccaaacggc actattctt gtaacaggg ggcgccggt tacgtaggc tctcacacc gttgtggaa ctactaaat aataattat tcagtaatt gccatcgac aatctcgtc aattgttac gccgaaaaa aaccaaaaa ccggagtcc ctcaaacgt gtcgaacaa atcacaaag aaacaaatc actttttac aatgttgac atccgaaac aaagaagct ttggacaag attttcaaa actcataaa atcgatgcc gttatccac tttgccgcc cttaaggcg gttggcgag tcagtccag gtgccccta atgtactac cagaacaac atagccggc tcgagcact ttatttgaa gtgatgcaa aataacggg gtgaaaaaa gttgttttt tcgtcgtct gctacagtt tacgggtta ccccaacat ttaccaatc acagaagcg catccgacc ggacaaaat tgtacaaat ccgtacgga aaaacgaaa tttttcgtt gaagaaatt ctgaaagat gtgtgcacg tcagatcct gaatggaaa gtcatttta ttgcgatac tttaacccc gtaggggca cacagctca ggtctcatt ggcgaggac ccctcagga atccccaac aatttaatg ccatatatt tctcaggta gctataggt cgtagggag aaagtgttt gtttttggc aacgactac tcaaccaaa gacggtacg ggagtccgt gactacatc catatcacg gacttggca attgggcat ttgaaggcg cttgaaaag atgaatgag gccgatttc aagggttgg aaggcctac aacttgggc acgggccaa ggttattca gtattagat ttgatcaat gcttttgcg aaagcctct ggtcaaaaa atcaactat gaagtgatc aaacgaaga gaaggggat attgcgagc agttttgcg gacccttct ctggccaaa aaagaactg aactggacg gcaacgcga ggcattgat gagatgtgt cgggacacg tggaattgg caaaaactg aacccgaac gggtttcag aagaaatga
- >TC009598 RB
atggctcca aacggcact attcttgta acagggggc gccggttac gtaggctct cacaccgtt gtggaacta ctaaataat aattattca gtaattgcc atcgacaat ctcgtcaat tgttacgcc gaaaaaaac caaaaaccg gagtccctc aaacgtgtc gaacaaatc acaaagaaa caaatcact ttttacaat gttgacatc cgaaacaaa gaagctttg gacaagatt ttcaaaact cataaaatc gatgccgtt atccacttt gccgccctt aaggcggtt ggcgagtca gtccaggtg cccctaatg tactaccag aacaacata gccggctcg agcacttta tttgaagtg atgcaaaat aacggggtg aaaaaagtt gttttttcg tcgtctgct acagtttac gggttaccc caacattta ccaatcaca gaagcgcat ccgaccgga caaaattgt acaaatccg tacggaaaa acgaaattt ttcgttgaa gaaattctg aaagatgtg tgcacgtca gatcctgaa tggaaagtc attttattg cgatacttt aaccccgta ggggcacac agctcaggt ctcattggc gaggacccc tcaggaatc cccaacaat ttaatgcca tatatttct caggtagct ataggtcgt agggagaaa gtgtttgtt tttggcaac gactactca accaaagac ggtacggga gtccgtgac tacatccat atcacggac ttggcaatt gggcatttg aaggcgctt gaaaagatg aatgaggcc gatttcaag ggttggaag gcctacaac ttgggcacg ggccaaggt tattcagta ttagatttg atcaatgct tttgcgaaa gcctctggt caaaaaatc aactatgaa gtgatcaaa cgaagagaa ggggatatt gcgagcagt tttgcggac ccttctctg gccaaaaaa gaactgaac tggacggca acgcgaggc attgatgag atgtgtcgg gacacgtgg aattggcaa aaactgaac ccgaacggg tttcagaag aaatga
- >TC009598 RA
tgggacgtc cgctgtcaa cgtcacatc ctaaataca aaaattttt aaacctctc aatagtgat aagaagtga taacaatgc tcgaatcat tgcgaaacc gcgtgtttg ttatttatt taagcgtgt ctgtgtatt acgtgataa ttccgatcg gcttcgtga caaattggt ggctatcag tggacgtaa tggctccaa acggcacta ttcttgtaa cagggggcg ccggttacg taggctctc acaccgttg tggaactac taaataata attattcag taattgcca tcgacaatc tcgtcaatt gttacgccg aaaaaaacc aaaaaccgg agtccctca aacgtgtcg aacaaatca caaagaaac aaatcactt tttacaatg ttgacatcc gaaacaaag aagctttgg acaagattt tcaaaactc ataaaatcg atgccgtta tccactttg ccgccctta aggcggttg gcgagtcag tccaggtgc ccctaatgt actaccaga acaacatag ccggctcga gcactttat ttgaagtga tgcaaaata acggggtga aaaaagttg ttttttcgt cgtctgcta cagtttacg ggttacccc aacatttac caatcacag aagcgcatc cgaccggac aaaattgta caaatccgt acggaaaaa cgaaatttt tcgttgaag aaattctga aagatgtgt gcacgtcag atcctgaat ggaaagtca ttttattgc gatacttta accccgtag gggcacaca gctcaggtc tcattggcg aggacccct caggaatcc ccaacaatt taatgccat atatttctc aggtagcta taggtcgta gggagaaag tgtttgttt ttggcaacg actactcaa ccaaagacg gtacgggag tccgtgact acatccata tcacggact tggcaattg ggcatttga aggcgcttg aaaagatga atgaggccg atttcaagg gttggaagg cctacaact tgggcacgg gccaaggtt attcagtat tagatttga tcaatgctt ttgcgaaag cctctggtc aaaaaatca actatgaag tgatcaaac gaagagaag gggatattg cgagcagtt ttgcggacc cttctctgg ccaaaaaag aactgaact ggacggcaa cgcgaggca ttgatgaga tgtgtcggg acacgtgga attggcaaa aactgaacc cgaacgggt ttcagaaga aatgatgca tttatgacg tatttagat ttttgttga gcaatgact ggtacttaa gcatttata cacccagta atttttgcc atattttat tctattgca tgttgaaaa taaaactat ttttctaat
- >TC009598 RB
aatacaaaa atttttaaa cctctcaat agtgataag aagtgataa caatgctcg aatcattgc gaaaccgcg tgtttgtta tttatttaa gcgtgtctg tgtattacg tgataattc cgatcggct tcgtgacaa agtaagttg gtggctatc agtggacgt aatggctcc aaacggcac tattcttgt aacaggggg cgccggtta cgtaggctc tcacaccgt tgtggaact actaaataa taattattc agtaattgc catcgacaa tctcgtcaa ttgttacgc cgaaaaaaa ccaaaaacc ggagtccct caaacgtgt cgaacaaat cacaaagaa acaaatcac tttttacaa tgttgacat ccgaaacaa agaagcttt ggacaagat tttcaaaac tcataaaat cgatgccgt tatccactt tgccgccct taaggcggt tggcgagtc agtccaggt gcccctaat gtactacca gaacaacat agccggctc gagcacttt atttgaagt gatgcaaaa taacggggt gaaaaaagt tgttttttc gtcgtctgc tacagttta cgggttacc ccaacattt accaatcac agaagcgca tccgaccgg acaaaattg tacaaatcc gtacggaaa aacgaaatt tttcgttga agaaattct gaaagatgt gtgcacgtc agatcctga atggaaagt cattttatt gcgatactt taaccccgt aggggcaca cagctcagg tctcattgg cgaggaccc ctcaggaat ccccaacaa tttaatgcc atatatttc tcaggtagc tataggtcg tagggagaa agtgtttgt ttttggcaa cgactactc aaccaaaga cggtacggg agtccgtga ctacatcca tatcacgga cttggcaat tgggcattt gaaggcgct tgaaaagat gaatgaggc cgatttcaa gggttggaa ggcctacaa cttgggcac gggccaagg ttattcagt attagattt gatcaatgc ttttgcgaa agcctctgg tcaaaaaat caactatga agtgatcaa acgaagaga aggggatat tgcgagcag ttttgcgga cccttctct ggccaaaaa agaactgaa ctggacggc aacgcgagg cattgatga gatgtgtcg ggacacgtg gaattggca aaaactgaa cccgaacgg gtttcagaa gaaatgatg catttatga cgtatttag atttttgtt gagcaatga ctggtactt aagcattta tacacccag taatttttg ccatatttt attctattg catgttgaa aataaaact atttttcta at