| General information | |||
| TC033574
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid47: 813683..843537 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033574 | |
| General information | ||||
| iB_02833 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC033574 RA
MNFHSGCVP KAAIIPPFV LILSKASAR PECLEKAVI CKGNKFPSC AELRVEESQ LLHGSGKVL IKYGFGDID YDGFRKFLD TFLEVTTPE ELCRHLFLS FVRKGVKVE GKAFKEMAV LSSTTACAP ITSHNKGST PNVNSIGEP IETKSQLSS EKPTTYGEM LVEKIHGFG EKLLGHHRS DSDCSTRGK TGTVHPMLT VTHTSYSCH EVLGKKSTD SSPSHSQIS RNSSKKSNN SLLVNNGKL EEVRHIVRK ASTLDVNTV KVSLKDIVC YLSLLEAGR PEDKLEFMF RLYDTDGNG VLDTNEMDC IVNQMMTVA EYLGWDVSR TQTYPAGHD GGDRLRRRR DCLAGGVEK GRFDHHPPI GAPGAGYEC ERRREPFME VEAFQQTGV LQPLLEYVG RVGQERVVL HVLLHNKCV AQVKAECGL GEHAVHILP PTAICPIVL DRQRSLQKR GSKYGHDNV GYPDFDPYS RSKTDVFQG NFANTTNKQ PAMSFQITT SPNSVPLLV FINPKSGGR QGARILRKF QYILNPRQV HSLASGGPM QGLSMFKDV PNFKVVCCG GDGTVGWVL ETMDKVELE CQPAVAVIP LGTGNDLAR CLRWGGGYE GESIHKILH KIARATTVL LDRWLIELS DTAQPDPDQ KIADTRIPY NIINNYFSI GVDAAICVK FHLEREKNP EKFNSRMKN KLWYFEYAT SEQFAASCK NLHEDIEIT CDDVSLDLA NGSPLQGIA LLNIPYTHG GSNLWGEHL SGSRRKSKK KKKQKEMST SSFNSVDLS VAVQDIGDG LIEVIGLEN CLHMGQVRT GLRASGRRL AQCSSVVIK TKKTFPMQI DGEPWMQPP TTIRITHKN QVPMLMAPP PEKGKGFFR FFKR
- >TC033574 RA
atgaatttt cattcgggt tgtgtacca aaggcggca attatacct ccattcgtc ttaattctc tcaaaagcg agtgctagg ccggaatgt ctcgagaaa gcggtcatt tgtaaaggg aataagttt ccgagttgt gctgaactc agagttgaa gagtcccag ctcttgcat ggttcgggg aaagtttta attaaatat ggatttgga gacatcgac tacgacggc ttccgcaag ttcctggac acatttctg gaggtgacc acccccgag gagctatgc cgccacctt ttcctctcc ttcgtccgt aaaggggta aaagtcgaa ggtaaagcg tttaaagaa atggccgta cttagttcc accactgct tgcgctcct atcacttct cataacaaa ggttccaca cccaacgtc aattccatc ggcgaaccc atcgagacc aaaagccaa ctctcttcg gaaaaacca acaacttac ggcgaaatg ctcgttgaa aaaatccac ggtttcggc gaaaaactc ttaggccac caccgatca gacagcgat tgttcgacc cgaggcaaa acaggcact gtgcaccca atgctgacc gtcacacac acttcctac tcgtgccac gaagtcttg gggaaaaaa agcaccgat tcctcaccc tcccatagc cagatttcc agaaattcg tcgaaaaaa tccaacaac tctctgctg gttaataac gggaagctg gaagaagtg cgccacatc gtgcgcaaa gccagcact ttggacgtc aacaccgtc aaagtctcg ctcaaagac atcgtgtgc tatctgtcc ctccttgag gccgggagg cccgaagac aagctggaa tttatgttc cggttgtat gacacggac ggaaacggc gttttggac accaacgag atggactgc atcgtaaac cagatgatg accgtggcg gaatatctc ggttgggac gtttcccga actcaaacc tatcctgca ggacatgat ggtggagat cgactacga cgccgacgg gactgtctc gctggagga gtggaaaag gggcggttt gaccaccat ccccctatt ggtgctcct ggggctgga tacgaatgt gaaagaaga cgggaacca tttatggag gttgaagca tttcagcag accggcgta ttgcaacct ctgcttgaa tatgttggt cgggttggg caagaaagg gttgtgctg cacgtgtta ctccataac aagtgcgtg gcccaggtg aaagccgag tgcggtttg ggcgagcac gccgtccac atcctgcct cccaccgcc atctgcccc atcgtcctg gaccgccag cgctctctc cagaaacgc ggctcgaaa tacggccac gacaatgtt ggttatccc gactttgat ccctattcc cgctcaaaa acagacgtt tttcagggc aatttcgcc aacaccacc aataaacaa ccggcgatg tccttccaa atcaccact tcgcccaat tccgtcccg ttattggtc tttatcaac cccaagtca gggggacgg caaggggcg aggatactc aggaagttt caatacatt ttgaacccg agacaggtc cacagcttg gccagcggc gggcccatg cagggcttg agcatgttc aaggacgtg cccaacttc aaggtcgtc tgctgcggg ggcgacggg accgtcgga tgggtgctg gagaccatg gacaaagtg gagttggaa tgtcagccg gccgtcgcc gtgattcct ttagggacc ggcaacgat ttggcgaga tgtttgcga tggggcggg ggctacgag ggcgaatcg atccacaaa attttacac aaaattgca agagcgacc accgttttg ctagacagg tggttgatc gaattatca gacaccgct caaccggac cctgatcaa aagatcgcc gacactagg atcccctac aacatcatc aacaactac ttctccatc ggagtggac gccgcaatt tgcgtcaaa tttcatctg gagcgcgag aaaaacccg gagaagttc aacagtcgg atgaagaat aagttgtgg tattttgaa tacgcgacg tcggagcag tttgcggct tcgtgtaaa aacctgcac gaggatatc gagattacg tgtgacgac gtgtccttg gacctggcg aacggctcc cccctgcag ggcatcgcc ctcctcaac atcccctac acgcacggc ggctcgaac ctctggggg gagcatttg tccggctcg cggcggaaa agtaaaaag aagaagaaa caaaaagaa atgagcact tctagtttc aattcggtc gatttgagt gtagccgtc caagatatc ggggacgga ttgattgag gttataggg ctggaaaat tgcctgcat atggggcaa gtgaggacc ggtttaagg gccagtgga cgccgactg gcgcagtgt tcttccgtc gttatcaaa acgaagaaa actttcccg atgcaaatc gacggcgag ccctggatg cagcccccg accactata cgtatcact cacaagaac caggtgccc atgctgatg gcgcccccg cccgagaag ggcaaaggc ttcttccgt ttcttcaag aggtag
- >TC033574 RA
cgattgata aacggctcc cgggggcgg caagccatc accgaaacg aaattgccg tgtaattgt gactggggg cggaaaaaa aaacatgaa ttttcattc gggttgtgt accaaaggc ggcaattat acctccatt cgtcttaat tctctcaaa agcgagtgc taggccgga atgtctcga gaaagcggt catttgtaa agggaataa gtttccgag ttgtgctga actcagagt tgaagagtc ccagctctt gcatggttc ggggaaagt tttaattaa atatggatt tggagacat cgactacga cggcttccg caagttcct ggacacatt tctggaggt gaccacccc cgaggagct atgccgcca ccttttcct ctccttcgt ccgtaaagg ggtaaaagt cgaaggtaa agcgtttaa agaaatggc cgtacttag ttccaccac tgcttgcgc tcctatcac ttctcataa caaaggttc cacacccaa cgtcaattc catcggcga acccatcga gaccaaaag ccaactctc ttcggaaaa accaacaac ttacggcga aatgctcgt tgaaaaaat ccacggttt cggcgaaaa actcttagg ccaccaccg atcagacag cgattgttc gacccgagg caaaacagg cactgtgca cccaatgct gaccgtcac acacacttc ctactcgtg ccacgaagt cttggggaa aaaaagcac cgattcctc accctccca tagccagat ttccagaaa ttcgtcgaa aaaatccaa caactctct gctggttaa taacgggaa gctggaaga agtgcgcca catcgtgcg caaagccag cactttgga cgtcaacac cgtcaaagt ctcgctcaa agacatcgt gtgctatct gtccctcct tgaggccgg gaggcccga agacaagct ggaatttat gttccggtt gtatgacac ggacggaaa cggcgtttt ggacaccaa cgagatgga ctgcatcgt aaaccagat gatgaccgt ggcggaata tctcggttg ggacgtttc ccgaactca aacctatcc tgcaggaca tgatggtgg agatcgact acgacgccg acgggactg tctcgctgg aggagtgga aaaggggcg gtttgacca ccatccccc tattggtgc tcctggggc tggatacga atgtgaaag aagacggga accatttat ggaggttga agcatttca gcagaccgg cgtattgca acctctgct tgaatatgt tggtcgggt tgggcaaga aagggttgt gctgcacgt gttactcca taacaagtg cgtggccca ggtgaaagc cgagtgcgg tttgggcga gcacgccgt ccacatcct gcctcccac cgccatctg ccccatcgt cctggaccg ccagcgctc tctccagaa acgcggctc gaaatacgg ccacgacaa tgttggtta tcccgactt tgatcccta ttcccgctc aaaaacaga cgtttttca gggcaattt cgccaacac caccaataa acaaccggc gatgtcctt ccaaatcac cacttcgcc caattccgt cccgttatt ggtctttat caaccccaa gtcaggggg acggcaagg ggcgaggat actcaggaa gtttcaata cattttgaa cccgagaca ggtccacag cttggccag cggcgggcc catgcaggg cttgagcat gttcaagga cgtgcccaa cttcaaggt cgtctgctg cgggggcga cgggaccgt cggatgggt gctggagac catggacaa agtggagtt ggaatgtca gccggccgt cgccgtgat tcctttagg gaccggcaa cgatttggc gagatgttt gcgatgggg cgggggcta cgagggcga atcgatcca caaaatttt acacaaaat tgcaagagc gaccaccgt tttgctaga caggtggtt gatcgaatt atcagacac cgctcaacc ggaccctga tcaaaagat cgccgacac taggatccc ctacaacat catcaacaa ctacttctc catcggagt ggacgccgc aatttgcgt caaatttca tctggagcg cgagaaaaa cccggagaa gttcaacag tcggatgaa gaataagtt gtggtattt tgaatacgc gacgtcgga gcagtttgc ggcttcgtg taaaaacct gcacgagga tatcgagat tacgtgtga cgacgtgtc cttggacct ggcgaacgg ctcccccct gcagggcat cgccctcct caacatccc ctacacgca cggcggctc gaacctctg gggggagca tttgtccgg ctcgcggcg gaaaagtaa aaagaagaa gaaacaaaa agaaatgag cacttctag tttcaattc ggtcgattt gagtgtagc cgtccaaga tatcgggga cggattgat tgaggttat agggctgga aaattgcct gcatatggg gcaagtgag gaccggttt aagggccag tggacgccg actggcgca gtgttcttc cgtcgttat caaaacgaa gaaaacttt cccgatgca aatcgacgg cgagccctg gatgcagcc cccgaccac tatacgtat cactcacaa gaaccaggt gcccatgct gatggcgcc cccgcccga gaagggcaa aggcttctt ccgtttctt caagaggta gagcggtga tattccaat tcctcgttt ctctgtagt cagttttct cgtttcgga atgacaata attaatttt gttcaatgt cagtattat ttatgaatg ggattactg aaaaaactc ttccaaaaa gcgctgttt attctatgt ttcaatgtt ccaagttgg tgttacgtg tttcgagca ttatccagc ataatgcca gctgctata tacaataaa tttttacaa agaaa