Status
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Gene information
General information
TC033574
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009583
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2895583..2925437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033574
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02833

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033574 RA
      MNFHSGCVP KAAIIPPFV LILSKASAR PECLEKAVI 
      CKGNKFPSC AELRVEESQ LLHGSGKVL IKYGFGDID 
      YDGFRKFLD TFLEVTTPE ELCRHLFLS FVRKGVKVE 
      GKAFKEMAV LSSTTACAP ITSHNKGST PNVNSIGEP 
      IETKSQLSS EKPTTYGEM LVEKIHGFG EKLLGHHRS 
      DSDCSTRGK TGTVHPMLT VTHTSYSCH EVLGKKSTD 
      SSPSHSQIS RNSSKKSNN SLLVNNGKL EEVRHIVRK 
      ASTLDVNTV KVSLKDIVC YLSLLEAGR PEDKLEFMF 
      RLYDTDGNG VLDTNEMDC IVNQMMTVA EYLGWDVSR 
      TQTYPAGHD GGDRLRRRR DCLAGGVEK GRFDHHPPI 
      GAPGAGYEC ERRREPFME VEAFQQTGV LQPLLEYVG 
      RVGQERVVL HVLLHNKCV AQVKAECGL GEHAVHILP 
      PTAICPIVL DRQRSLQKR GSKYGHDNV GYPDFDPYS 
      RSKTDVFQG NFANTTNKQ PAMSFQITT SPNSVPLLV 
      FINPKSGGR QGARILRKF QYILNPRQV HSLASGGPM 
      QGLSMFKDV PNFKVVCCG GDGTVGWVL ETMDKVELE 
      CQPAVAVIP LGTGNDLAR CLRWGGGYE GESIHKILH 
      KIARATTVL LDRWLIELS DTAQPDPDQ KIADTRIPY 
      NIINNYFSI GVDAAICVK FHLEREKNP EKFNSRMKN 
      KLWYFEYAT SEQFAASCK NLHEDIEIT CDDVSLDLA 
      NGSPLQGIA LLNIPYTHG GSNLWGEHL SGSRRKSKK 
      KKKQKEMST SSFNSVDLS VAVQDIGDG LIEVIGLEN 
      CLHMGQVRT GLRASGRRL AQCSSVVIK TKKTFPMQI 
      DGEPWMQPP TTIRITHKN QVPMLMAPP PEKGKGFFR 
      FFKR
    CDS sequence
    • >TC033574 RA
      atgaatttt cattcgggt tgtgtacca aaggcggca 
      attatacct ccattcgtc ttaattctc tcaaaagcg 
      agtgctagg ccggaatgt ctcgagaaa gcggtcatt 
      tgtaaaggg aataagttt ccgagttgt gctgaactc 
      agagttgaa gagtcccag ctcttgcat ggttcgggg 
      aaagtttta attaaatat ggatttgga gacatcgac 
      tacgacggc ttccgcaag ttcctggac acatttctg 
      gaggtgacc acccccgag gagctatgc cgccacctt 
      ttcctctcc ttcgtccgt aaaggggta aaagtcgaa 
      ggtaaagcg tttaaagaa atggccgta cttagttcc 
      accactgct tgcgctcct atcacttct cataacaaa 
      ggttccaca cccaacgtc aattccatc ggcgaaccc 
      atcgagacc aaaagccaa ctctcttcg gaaaaacca 
      acaacttac ggcgaaatg ctcgttgaa aaaatccac 
      ggtttcggc gaaaaactc ttaggccac caccgatca 
      gacagcgat tgttcgacc cgaggcaaa acaggcact 
      gtgcaccca atgctgacc gtcacacac acttcctac 
      tcgtgccac gaagtcttg gggaaaaaa agcaccgat 
      tcctcaccc tcccatagc cagatttcc agaaattcg 
      tcgaaaaaa tccaacaac tctctgctg gttaataac 
      gggaagctg gaagaagtg cgccacatc gtgcgcaaa 
      gccagcact ttggacgtc aacaccgtc aaagtctcg 
      ctcaaagac atcgtgtgc tatctgtcc ctccttgag 
      gccgggagg cccgaagac aagctggaa tttatgttc 
      cggttgtat gacacggac ggaaacggc gttttggac 
      accaacgag atggactgc atcgtaaac cagatgatg 
      accgtggcg gaatatctc ggttgggac gtttcccga 
      actcaaacc tatcctgca ggacatgat ggtggagat 
      cgactacga cgccgacgg gactgtctc gctggagga 
      gtggaaaag gggcggttt gaccaccat ccccctatt 
      ggtgctcct ggggctgga tacgaatgt gaaagaaga 
      cgggaacca tttatggag gttgaagca tttcagcag 
      accggcgta ttgcaacct ctgcttgaa tatgttggt 
      cgggttggg caagaaagg gttgtgctg cacgtgtta 
      ctccataac aagtgcgtg gcccaggtg aaagccgag 
      tgcggtttg ggcgagcac gccgtccac atcctgcct 
      cccaccgcc atctgcccc atcgtcctg gaccgccag 
      cgctctctc cagaaacgc ggctcgaaa tacggccac 
      gacaatgtt ggttatccc gactttgat ccctattcc 
      cgctcaaaa acagacgtt tttcagggc aatttcgcc 
      aacaccacc aataaacaa ccggcgatg tccttccaa 
      atcaccact tcgcccaat tccgtcccg ttattggtc 
      tttatcaac cccaagtca gggggacgg caaggggcg 
      aggatactc aggaagttt caatacatt ttgaacccg 
      agacaggtc cacagcttg gccagcggc gggcccatg 
      cagggcttg agcatgttc aaggacgtg cccaacttc 
      aaggtcgtc tgctgcggg ggcgacggg accgtcgga 
      tgggtgctg gagaccatg gacaaagtg gagttggaa 
      tgtcagccg gccgtcgcc gtgattcct ttagggacc 
      ggcaacgat ttggcgaga tgtttgcga tggggcggg 
      ggctacgag ggcgaatcg atccacaaa attttacac 
      aaaattgca agagcgacc accgttttg ctagacagg 
      tggttgatc gaattatca gacaccgct caaccggac 
      cctgatcaa aagatcgcc gacactagg atcccctac 
      aacatcatc aacaactac ttctccatc ggagtggac 
      gccgcaatt tgcgtcaaa tttcatctg gagcgcgag 
      aaaaacccg gagaagttc aacagtcgg atgaagaat 
      aagttgtgg tattttgaa tacgcgacg tcggagcag 
      tttgcggct tcgtgtaaa aacctgcac gaggatatc 
      gagattacg tgtgacgac gtgtccttg gacctggcg 
      aacggctcc cccctgcag ggcatcgcc ctcctcaac 
      atcccctac acgcacggc ggctcgaac ctctggggg 
      gagcatttg tccggctcg cggcggaaa agtaaaaag 
      aagaagaaa caaaaagaa atgagcact tctagtttc 
      aattcggtc gatttgagt gtagccgtc caagatatc 
      ggggacgga ttgattgag gttataggg ctggaaaat 
      tgcctgcat atggggcaa gtgaggacc ggtttaagg 
      gccagtgga cgccgactg gcgcagtgt tcttccgtc 
      gttatcaaa acgaagaaa actttcccg atgcaaatc 
      gacggcgag ccctggatg cagcccccg accactata 
      cgtatcact cacaagaac caggtgccc atgctgatg 
      gcgcccccg cccgagaag ggcaaaggc ttcttccgt 
      ttcttcaag aggtag
    mRNA sequence
    • >TC033574 RA
      cgattgata aacggctcc cgggggcgg caagccatc 
      accgaaacg aaattgccg tgtaattgt gactggggg 
      cggaaaaaa aaacatgaa ttttcattc gggttgtgt 
      accaaaggc ggcaattat acctccatt cgtcttaat 
      tctctcaaa agcgagtgc taggccgga atgtctcga 
      gaaagcggt catttgtaa agggaataa gtttccgag 
      ttgtgctga actcagagt tgaagagtc ccagctctt 
      gcatggttc ggggaaagt tttaattaa atatggatt 
      tggagacat cgactacga cggcttccg caagttcct 
      ggacacatt tctggaggt gaccacccc cgaggagct 
      atgccgcca ccttttcct ctccttcgt ccgtaaagg 
      ggtaaaagt cgaaggtaa agcgtttaa agaaatggc 
      cgtacttag ttccaccac tgcttgcgc tcctatcac 
      ttctcataa caaaggttc cacacccaa cgtcaattc 
      catcggcga acccatcga gaccaaaag ccaactctc 
      ttcggaaaa accaacaac ttacggcga aatgctcgt 
      tgaaaaaat ccacggttt cggcgaaaa actcttagg 
      ccaccaccg atcagacag cgattgttc gacccgagg 
      caaaacagg cactgtgca cccaatgct gaccgtcac 
      acacacttc ctactcgtg ccacgaagt cttggggaa 
      aaaaagcac cgattcctc accctccca tagccagat 
      ttccagaaa ttcgtcgaa aaaatccaa caactctct 
      gctggttaa taacgggaa gctggaaga agtgcgcca 
      catcgtgcg caaagccag cactttgga cgtcaacac 
      cgtcaaagt ctcgctcaa agacatcgt gtgctatct 
      gtccctcct tgaggccgg gaggcccga agacaagct 
      ggaatttat gttccggtt gtatgacac ggacggaaa 
      cggcgtttt ggacaccaa cgagatgga ctgcatcgt 
      aaaccagat gatgaccgt ggcggaata tctcggttg 
      ggacgtttc ccgaactca aacctatcc tgcaggaca 
      tgatggtgg agatcgact acgacgccg acgggactg 
      tctcgctgg aggagtgga aaaggggcg gtttgacca 
      ccatccccc tattggtgc tcctggggc tggatacga 
      atgtgaaag aagacggga accatttat ggaggttga 
      agcatttca gcagaccgg cgtattgca acctctgct 
      tgaatatgt tggtcgggt tgggcaaga aagggttgt 
      gctgcacgt gttactcca taacaagtg cgtggccca 
      ggtgaaagc cgagtgcgg tttgggcga gcacgccgt 
      ccacatcct gcctcccac cgccatctg ccccatcgt 
      cctggaccg ccagcgctc tctccagaa acgcggctc 
      gaaatacgg ccacgacaa tgttggtta tcccgactt 
      tgatcccta ttcccgctc aaaaacaga cgtttttca 
      gggcaattt cgccaacac caccaataa acaaccggc 
      gatgtcctt ccaaatcac cacttcgcc caattccgt 
      cccgttatt ggtctttat caaccccaa gtcaggggg 
      acggcaagg ggcgaggat actcaggaa gtttcaata 
      cattttgaa cccgagaca ggtccacag cttggccag 
      cggcgggcc catgcaggg cttgagcat gttcaagga 
      cgtgcccaa cttcaaggt cgtctgctg cgggggcga 
      cgggaccgt cggatgggt gctggagac catggacaa 
      agtggagtt ggaatgtca gccggccgt cgccgtgat 
      tcctttagg gaccggcaa cgatttggc gagatgttt 
      gcgatgggg cgggggcta cgagggcga atcgatcca 
      caaaatttt acacaaaat tgcaagagc gaccaccgt 
      tttgctaga caggtggtt gatcgaatt atcagacac 
      cgctcaacc ggaccctga tcaaaagat cgccgacac 
      taggatccc ctacaacat catcaacaa ctacttctc 
      catcggagt ggacgccgc aatttgcgt caaatttca 
      tctggagcg cgagaaaaa cccggagaa gttcaacag 
      tcggatgaa gaataagtt gtggtattt tgaatacgc 
      gacgtcgga gcagtttgc ggcttcgtg taaaaacct 
      gcacgagga tatcgagat tacgtgtga cgacgtgtc 
      cttggacct ggcgaacgg ctcccccct gcagggcat 
      cgccctcct caacatccc ctacacgca cggcggctc 
      gaacctctg gggggagca tttgtccgg ctcgcggcg 
      gaaaagtaa aaagaagaa gaaacaaaa agaaatgag 
      cacttctag tttcaattc ggtcgattt gagtgtagc 
      cgtccaaga tatcgggga cggattgat tgaggttat 
      agggctgga aaattgcct gcatatggg gcaagtgag 
      gaccggttt aagggccag tggacgccg actggcgca 
      gtgttcttc cgtcgttat caaaacgaa gaaaacttt 
      cccgatgca aatcgacgg cgagccctg gatgcagcc 
      cccgaccac tatacgtat cactcacaa gaaccaggt 
      gcccatgct gatggcgcc cccgcccga gaagggcaa 
      aggcttctt ccgtttctt caagaggta gagcggtga 
      tattccaat tcctcgttt ctctgtagt cagttttct 
      cgtttcgga atgacaata attaatttt gttcaatgt 
      cagtattat ttatgaatg ggattactg aaaaaactc 
      ttccaaaaa gcgctgttt attctatgt ttcaatgtt 
      ccaagttgg tgttacgtg tttcgagca ttatccagc 
      ataatgcca gctgctata tacaataaa tttttacaa 
      agaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY