Status
Please wait ...
Gene information
General information
TC009536 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2186947..2190771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009536
    iBeetle screen iB_02832

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    remarks - cuticles: eggs lost,
    Should be repeated.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009536 RA
      MIKGPMDEI EWNVAHEGQ LLEAMVGHK 
PVGVNKYFQ MACICDKFA DSCQKEINS 
DKVWAHLET MYNLEALDE SESIPFPNS 
EKEFTLPDS DFGSLIVKK EEDKKLAQK 
GRETPKSLK ELRKDEKTP VRSSKEVPR 
RDSKDSKDK TPVSAKKEI KKEAEKSKS 
KGKSNSSTP REENKAKAK SDDTPKLTK 
RPTRGSSKP DDSSSSGKS SPITVTPPV 
TKRRRI
    CDS sequence
    • >TC009536 RA
      atgattaag ggccccatg gacgagatc gagtggaac 
gtggcgcac gaggggcag ctcctggag gccatggtg 
gggcacaag cccgtcggc gtcaataag tacttccaa 
atggcgtgc atctgtgac aagttcgct gatagttgc 
cagaaggag atcaactcg gacaaggtg tgggcccac 
ctcgagaca atgtacaat ttggaagct ttggatgag 
agtgagtcg attccgttc ccgaatagt gagaaagag 
ttcacactt cccgattcc gacttcggt tccctcatt 
gtgaagaag gaagaggac aagaaattg gcgcagaaa 
gggcgcgaa acgcccaag tcgctcaag gaattgcga 
aaagatgaa aagactcct gtgaggagc agtaaggag 
gtgccaagg cgtgatagt aaagacagt aaggacaaa 
accccagtg agtgcgaaa aaagaaatc aagaaagag 
gctgaaaag agtaaaagt aaaggtaag agtaatagt 
agtacacca agggaggaa aacaaggca aaagcgaaa 
agtgacgac acaccaaaa ctgacgaag cgccctact 
agaggcagc tctaaacct gacgattcc agttcgagt 
ggtaaatct agtccaatc acagtcaca ccaccagtg 
acaaaaagg cgacgcatt tag
    mRNA sequence
    • >TC009536 RA
      gccttcttc aaaatggct ggcacaaat cgctaacaa 
ggcaataag cagtatgta atacaagcc gcgatggct 
ttttacggc cgcgattag tgtcaacac gtccataat 
cacaagtca cggtttatt gccctcttt tcgccccct 
ttcggaagt gaaggtccc ggggcccgt ttaattgca 
aaataacca aaagcagcg ttacataaa agctggagc 
ctccagcgt tcacgagtt cagtcattt ttggccgtt 
ttttcctcc atctctgtc tcgatcgat tcttgaagg 
caaggctga acaaggcaa cgctggatt atataccgg 
tgttgtaga agagccgcc tgacaagat accaggcaa 
tgaccgcaa ccagaacgt tgaaccgaa gactccatt 
tcaccggag cggctaatt cagttccgg gatgtcttg 
tttatttat ttgggcccc gaatcaacg ccggggacc 
ggacccaca ttgctcaca atcattcca agcacggcc 
cacacccga agcgaattc cgagctcca caagtccgg 
ctctgttcc cgtctcgga cttttcagg acgaaaaaa 
cgtgatttg aatcaaatt gcgccaaaa tttgacaag 
agcaccacg cacacggcg ccaaattcc ccaaaccgg 
cagttttaa attgaaact ttggacaaa ctcttcagt 
aaatcattt accaacatt gtagctcgt gtttttaat 
aactatcgg tactttgac gagctcaaa ttaaatttt 
ttttaagga aaaaggaaa atacgggat tcagggtca 
caaacaaaa aaacgtgtg tcaagactt gaatgcatt 
tattttgca aaaaaaaaa tgttgaagt gttctaaca 
acattctaa tgtagttga taatacatc tgtggcctg 
taaaacaat aatgactaa aagattaat taaaatttc 
aaattgacc gtgatggcg gcagtggcg ccctcaaac 
agtcatttg tcaattggc gttttaaat aatttaatt 
ccgttttta aatgattaa gggccccat ggacgagat 
cgagtggaa cgtggcgca cgaggggca gctcctgga 
ggccatggt ggggcacaa gcccgtcgg cgtcaataa 
gtacttcca aatggcgtg catctgtga caagttcgc 
tgatagttg ccagaagga gatcaactc ggacaaggt 
gtgggccca cctcgagac aatgtacaa tttggaagc 
tttggatga gagtgagtc gattccgtt cccgaatag 
tgagaaaga gttcacact tcccgattc cgacttcgg 
ttccctcat tgtgaagaa ggaagagga caagaaatt 
ggcgcagaa agggcgcga aacgcccaa gtcgctcaa 
ggaattgcg aaaagatga aaagactcc tgtgaggag 
cagtaagga ggtgccaag gcgtgatag taaagacag 
taaggacaa aaccccagt gagtgcgaa aaaagaaat 
caagaaaga ggctgaaaa gagtaaaag taaaggtaa 
gagtaatag tagtacacc aagggagga aaacaaggc 
aaaagcgaa aagtgacga cacaccaaa actgacgaa 
gcgccctac tagaggcag ctctaaacc tgacgattc 
cagttcgag tggtaaatc tagtccaat cacagtcac 
accaccagt gacaaaaag gcgacgcat ttagtttct 
ttctcactt tattaaaat taataaaaa gtgcctcaa 
taaattacg taaaacacg atgcaactc ggagcaaca 
tcacctctc aaggcggca gaaaaacgc atgtttcga 
ttcgcttgt ctcgcgtaa atacttatc ataaaaatt 
caaaacgac tgcttccac cttaaaacc gaacttttg 
gtacaaaaa caaggccgg gttgttgat ggacacgtg 
tagtgtgat atctttgcc gagacttgt ctga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY