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Gene information
General information
TC033516 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 1230190..1237483
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033516
    iBeetle screen iB_02828
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033516 RA
      MFQHLKLLL WKNLVIRKR HWLLTLTEI LIPILLFTL 
      VAYGRSKIS GLNKQVIDF PTYYRENTL DEIYSHLDV 
      GELKLWYSP YNDFTGKII RKVQETFQI PGDDVREFT 
      SAEKLEQHF QTISNSSAL ITVVNFHGN DPKHLDYDL 
      SLYDKYLLW DTDKLFLPV INQEDRRSS KYIYKGFAA 
      LQVAIDLAF LELQTGQKP KIDFSIQEF PDPPHTDDS 
      GLSQLFLYF LPLITIFSF IFLCPAVLQ RVGEEKHSG 
      TKEFMKMVG LNSSMLWLG WFIHALITN LFSIIVIVV 
      LMKVPFWGV AYPPIEYSS GVVLFVFLF LYCMAAITF 
      CFLIATIIS KPSIATVVG LLIWIFSYF IPQALVTSH 
      ENLAWRFKI PLAIFPNMA LHFGYNSIS VYEMRELGI 
      QWSNIFHSG SGGENDVTM GNVFVMLIV DMIFFMLLT 
      IYIENVKPG KYGIAQPYT FPLKSLMNC VRCFGCFTK 
      IDNDPSTKR LDAENGPPV CIEICELFK KFKNVVAVE 
      NLSLNIYKN HITVLLGHN GAGKTTTMS ILTGMINAT 
      NGYVKINGK DIKQNTSEV RRLLGLCPQ HNLLFPDLT 
      VFEHLKFFA MIKRSKNST KEAKEMLEQ LGLEEKKYD 
      MACTLSGGM KRKLCLGMA LIGDSQVLI LDEPTSGMD 
      PESRRKVWN MLLEYRKSK TILITTHFM EEADVLGDY 
      IAIMADGRL QDSDTPYNL KKKYNTGYH MSLMLESLD 
      FKKEITGEI RRHIPEAQL IRTHHDTLV YLLPMDRKS 
      KYKDVFVSL EDKKADWKI VTIGLSLTT LNDVFLKAR 
      DKLLGIEHS ETDGLSKPE FSTQKTKKL NRSLNSFLA 
      LIVKRWYFL EKKFYWYFP AVIVSITIF LLAIWLGIS 
      STDYSNVAE PQLNLSLKV YDKSYAYFG GDNLTELVK 
      MRQFYEKIV KQQDGYPEL HKVVSEGII QAGTKNIAF 
      YNKHMIVAA EFNQSSNDE IVVNAMYNR FAIHGVPIS 
      INLAMNAAV KALLNETYS ITTSNTPLK SLSSELTPA 
      ELSVVSVAS VWVILIPMS KFKIAPQTR LARSLVKET 
      VNND
    • >TC033516 RB
      MKGENKFDL CFRTDSMFQ HLKLLLWKN LVIRKRHWL 
      LTLTEILIP ILLFTLVAY GRSKISGLN KQVIDFPTY 
      YRENTLDEI YSHLDVGEL KLWYSPYND FTGKIIRKV 
      QETFQIPGD DVREFTSAE KLEQHFQTI SNSSALITV 
      VNFHGNDPK HLDYDLSLY DKYLLWDTD KLFLPVINQ 
      EDRRSSKYI YKGFAALQV AIDLAFLEL QTGQKPKID 
      FSIQEFPDP PHTDDSGLS QLFLYFLPL ITIFSFIFL 
      CPAVLQRVG EEKHSGTKE FMKMVGLNS SMLWLGWFI 
      HALITNLFS IIVIVVLMK VPFWGVAYP PIEYSSGVV 
      LFVFLFLYC MAAITFCFL IATIISKPS IATVVGLLI 
      WIFSYFIPQ ALVTSHENL AWRFKIPLA IFPNMALHF 
      GYNSISVYE MRELGIQWS NIFHSGSGG ENDVTMGNV 
      FVMLIVDMI FFMLLTIYI ENVKPGKYG IAQPYTFPL 
      KSLMNCVRC FGCFTKIDN DPSTKRLDA ENGPPVCIE 
      ICELFKKFK NVVAVENLS LNIYKNHIT VLLGHNGAG 
      KTTTMSILT GMINATNGY VKINGKDIK QNTSEVRRL 
      LGLCPQHNL LFPDLTVFE HLKFFAMIK RSKNSTKEA 
      KEMLEQLGL EEKKYDMAC TLSGGMKRK LCLGMALIG 
      DSQVLILDE PTSGMDPES RRKVWNMLL EYRKSKTIL 
      ITTHFMEEA DVLGDYIAI MADGRLQDS DTPYNLKKK 
      YNTGYHMSL MLESLDFKK EITGEIRRH IPEAQLIRT 
      HHDTLVYLL PMDRKSKYK DVFVSLEDK KADWKIVTI 
      GLSLTTLND VFLKARDKL LGIEHSETD GLSKPEFST 
      QKTKKLNRS LNSFLALIV KRWYFLEKK FYWYFPAVI 
      VSITIFLLA IWLGISSTD YSNVAEPQL NLSLKVYDK 
      SYAYFGGDN LTELVKMRQ FYEKIVKQQ DGYPELHKV 
      VSEGIIQAG TKNIAFYNK HMIVAAEFN QSSNDEIVV 
      NAMYNRFAI HGVPISINL AMNAAVKAL LNETYSITT 
      SNTPLKSLS SELTPAELS VVSVASVWV ILIPMSKFK 
      IAPQTRLAR SLVKETVNN D
    CDS sequence
    • >TC033516 RA
      atgtttcaa cacttgaag ttattacta tggaaaaac 
      ttagtcatc aggaagagg cactggctt ttaacatta 
      accgaaatc ttaattccc attttattg ttcacttta 
      gtcgcttac ggccgatct aaaataagc ggcttgaac 
      aaacaagtc atcgatttt ccaacgtac tatcgcgaa 
      aatacgctg gacgagatc tactctcac ttagatgtt 
      ggggaactg aaactgtgg tattcgccc tacaatgat 
      tttactgga aaaatcata agaaaagtc caggaaacg 
      ttccaaatt cccggcgat gatgtgagg gagttcact 
      tcggccgag aaactcgag cagcatttt cagaccatt 
      tctaactct tccgctttg ataacagtg gttaatttt 
      cacggaaac gaccccaaa catctggat tatgaccta 
      agcctttac gataagtac ttattatgg gacaccgac 
      aagcttttc ctccccgtc ataaaccag gaagaccga 
      agatcgtca aaatacatc tacaagggc ttcgctgca 
      cttcaggtg gcaatagac ttggcattt ttggaactc 
      caaaccggg caaaaacca aaaatcgat ttttccatt 
      caggaattt ccagacccg cctcacact gacgattcg 
      ggcttgagc caacttttc ttgtatttc ctgcctttg 
      ataaccatc ttcagcttc atttttttg tgtccagct 
      gtgttgcaa cgagtgggt gaagagaaa cattcagga 
      actaaggag tttatgaaa atggtcggc ctgaactcg 
      tccatgtta tggctgggt tggtttatt catgcttta 
      ataaccaat ctattctcc atcattgtt atcgtggtt 
      ttgatgaaa gtacctttc tggggtgtt gcctatcca 
      ccgattgaa tattcaagt ggagtcgtt ctcttcgtg 
      tttttgttc ttgtactgt atggctgcc atcacgttc 
      tgtttcctc attgctacc ataatcagc aaaccatca 
      attgcgaca gtggtgggc ttactgatt tggattttt 
      tcgtacttc ataccgcaa gctttagta acatcgcac 
      gaaaacctg gcctggcgg tttaaaata cctttggcg 
      atcttccct aacatggca ttgcatttc ggatacaac 
      tcaatttca gtttacgaa atgagagag ttaggcatc 
      caatggtcc aacattttc cactcgggg agcggcggt 
      gaaaatgac gtaacaatg ggaaacgtt ttcgtgatg 
      ttgattgtg gacatgatt ttcttcatg ttattaacg 
      atctacatc gaaaacgtt aaaccgggg aagtacgga 
      atcgcccag ccttacact ttcccgtta aaatctctg 
      atgaattgc gttagatgc tttggttgt tttaccaaa 
      attgacaat gacccatcc acaaaacgt ttggatgca 
      gagaatgga ccaccagtt tgtattgaa atttgcgag 
      ctatttaaa aagttcaaa aacgttgtt gccgttgaa 
      aacctcagt ttgaacatt tacaagaat cacatcaca 
      gttttgttg ggtcataat ggggctgga aaaaccaca 
      acaatgtcc atcctcaca gggatgatc aatgcgaca 
      aatggttat gttaaaatc aacggaaaa gatatcaag 
      caaaacacc agcgaggtc agaagactg ctaggtctt 
      tgcccacaa cacaatctc ctgtttccc gacttgacc 
      gtcttcgaa catttgaaa tttttcgca atgataaag 
      cggtcgaaa aactcaacc aaagaggcc aaagaaatg 
      ttagaacaa ttgggtctc gaggagaaa aaatacgat 
      atggcttgt actttgtcg ggtgggatg aagcgcaag 
      ctatgtctt ggcatggcg ctaatcggg gattcacag 
      gtattgatt ttggacgaa ccgacgtcc ggtatggac 
      ccagagtcg cgtcgtaaa gtgtggaac atgttgctg 
      gagtacaga aaatcaaag accatccta atcacgacc 
      cattttatg gaggaagct gatgttctt ggcgactat 
      atcgctata atggctgat ggacggttg caagattct 
      gatacaccg tacaatttg aaaaagaaa tacaatacc 
      ggttaccac atgtctcta atgcttgag tcactcgac 
      tttaagaaa gaaataaca ggggaaata cggcgccac 
      attcctgaa gcacaattg attcgtaca catcatgac 
      actctggtt tatttgtta ccgatggat aggaaatct 
      aagtataaa gatgttttt gtttctttg gaagacaaa 
      aaagctgat tggaaaatt gttacgatc gggctcagt 
      ttgaccaca cttaacgat gtttttcta aaagccagg 
      gacaaactt ctgggaatc gaacacagt gaaaccgac 
      gggttaagc aaaccagag tttagtacg caaaaaacg 
      aagaaattg aatcgttcg ttgaacagt tttctggcg 
      ctgattgtc aaacgttgg tatttctta gagaaaaaa 
      ttctattgg tactttccc gccgtgatt gtatcaatt 
      acgattttc ctgctcgcg atctggtta gggatctca 
      agcactgac tatagcaac gtcgctgag ccccaattg 
      aacctaagc cttaaagtc tacgacaaa tcgtacgct 
      tatttcggc ggtgataat ttaaccgag ttggttaaa 
      atgagacag ttttacgag aaaatagtc aaacaacaa 
      gacggttat cccgaattg cataaagtc gtgtctgaa 
      ggcatcatc caagcagga acgaaaaat atagcgttt 
      tacaacaaa cacatgatc gtggctgca gaatttaat 
      caaagttca aacgatgag atcgttgtt aatgccatg 
      tacaatcgt ttcgcgata catggagtt ccaatttcc 
      attaatttg gctatgaat gcagcggtt aaagctctc 
      ctaaacgaa acttattca attacgaca agtaatacg 
      ccgcttaaa tcactcagt tcggagtta actccagcc 
      gaattgtcg gtcgttagt gtggccagc gtttgggtg 
      atcctcata ccgatgagt aagttcaaa attgcgcct 
      cagacaaga cttgcgcgc tctctggtg aaagaaacg 
      gttaataat gattga
    • >TC033516 RB
      atgaagggg gaaaacaag ttcgatttg tgtttcaga 
      accgactca atgtttcaa cacttgaag ttattacta 
      tggaaaaac ttagtcatc aggaagagg cactggctt 
      ttaacatta accgaaatc ttaattccc attttattg 
      ttcacttta gtcgcttac ggccgatct aaaataagc 
      ggcttgaac aaacaagtc atcgatttt ccaacgtac 
      tatcgcgaa aatacgctg gacgagatc tactctcac 
      ttagatgtt ggggaactg aaactgtgg tattcgccc 
      tacaatgat tttactgga aaaatcata agaaaagtc 
      caggaaacg ttccaaatt cccggcgat gatgtgagg 
      gagttcact tcggccgag aaactcgag cagcatttt 
      cagaccatt tctaactct tccgctttg ataacagtg 
      gttaatttt cacggaaac gaccccaaa catctggat 
      tatgaccta agcctttac gataagtac ttattatgg 
      gacaccgac aagcttttc ctccccgtc ataaaccag 
      gaagaccga agatcgtca aaatacatc tacaagggc 
      ttcgctgca cttcaggtg gcaatagac ttggcattt 
      ttggaactc caaaccggg caaaaacca aaaatcgat 
      ttttccatt caggaattt ccagacccg cctcacact 
      gacgattcg ggcttgagc caacttttc ttgtatttc 
      ctgcctttg ataaccatc ttcagcttc atttttttg 
      tgtccagct gtgttgcaa cgagtgggt gaagagaaa 
      cattcagga actaaggag tttatgaaa atggtcggc 
      ctgaactcg tccatgtta tggctgggt tggtttatt 
      catgcttta ataaccaat ctattctcc atcattgtt 
      atcgtggtt ttgatgaaa gtacctttc tggggtgtt 
      gcctatcca ccgattgaa tattcaagt ggagtcgtt 
      ctcttcgtg tttttgttc ttgtactgt atggctgcc 
      atcacgttc tgtttcctc attgctacc ataatcagc 
      aaaccatca attgcgaca gtggtgggc ttactgatt 
      tggattttt tcgtacttc ataccgcaa gctttagta 
      acatcgcac gaaaacctg gcctggcgg tttaaaata 
      cctttggcg atcttccct aacatggca ttgcatttc 
      ggatacaac tcaatttca gtttacgaa atgagagag 
      ttaggcatc caatggtcc aacattttc cactcgggg 
      agcggcggt gaaaatgac gtaacaatg ggaaacgtt 
      ttcgtgatg ttgattgtg gacatgatt ttcttcatg 
      ttattaacg atctacatc gaaaacgtt aaaccgggg 
      aagtacgga atcgcccag ccttacact ttcccgtta 
      aaatctctg atgaattgc gttagatgc tttggttgt 
      tttaccaaa attgacaat gacccatcc acaaaacgt 
      ttggatgca gagaatgga ccaccagtt tgtattgaa 
      atttgcgag ctatttaaa aagttcaaa aacgttgtt 
      gccgttgaa aacctcagt ttgaacatt tacaagaat 
      cacatcaca gttttgttg ggtcataat ggggctgga 
      aaaaccaca acaatgtcc atcctcaca gggatgatc 
      aatgcgaca aatggttat gttaaaatc aacggaaaa 
      gatatcaag caaaacacc agcgaggtc agaagactg 
      ctaggtctt tgcccacaa cacaatctc ctgtttccc 
      gacttgacc gtcttcgaa catttgaaa tttttcgca 
      atgataaag cggtcgaaa aactcaacc aaagaggcc 
      aaagaaatg ttagaacaa ttgggtctc gaggagaaa 
      aaatacgat atggcttgt actttgtcg ggtgggatg 
      aagcgcaag ctatgtctt ggcatggcg ctaatcggg 
      gattcacag gtattgatt ttggacgaa ccgacgtcc 
      ggtatggac ccagagtcg cgtcgtaaa gtgtggaac 
      atgttgctg gagtacaga aaatcaaag accatccta 
      atcacgacc cattttatg gaggaagct gatgttctt 
      ggcgactat atcgctata atggctgat ggacggttg 
      caagattct gatacaccg tacaatttg aaaaagaaa 
      tacaatacc ggttaccac atgtctcta atgcttgag 
      tcactcgac tttaagaaa gaaataaca ggggaaata 
      cggcgccac attcctgaa gcacaattg attcgtaca 
      catcatgac actctggtt tatttgtta ccgatggat 
      aggaaatct aagtataaa gatgttttt gtttctttg 
      gaagacaaa aaagctgat tggaaaatt gttacgatc 
      gggctcagt ttgaccaca cttaacgat gtttttcta 
      aaagccagg gacaaactt ctgggaatc gaacacagt 
      gaaaccgac gggttaagc aaaccagag tttagtacg 
      caaaaaacg aagaaattg aatcgttcg ttgaacagt 
      tttctggcg ctgattgtc aaacgttgg tatttctta 
      gagaaaaaa ttctattgg tactttccc gccgtgatt 
      gtatcaatt acgattttc ctgctcgcg atctggtta 
      gggatctca agcactgac tatagcaac gtcgctgag 
      ccccaattg aacctaagc cttaaagtc tacgacaaa 
      tcgtacgct tatttcggc ggtgataat ttaaccgag 
      ttggttaaa atgagacag ttttacgag aaaatagtc 
      aaacaacaa gacggttat cccgaattg cataaagtc 
      gtgtctgaa ggcatcatc caagcagga acgaaaaat 
      atagcgttt tacaacaaa cacatgatc gtggctgca 
      gaatttaat caaagttca aacgatgag atcgttgtt 
      aatgccatg tacaatcgt ttcgcgata catggagtt 
      ccaatttcc attaatttg gctatgaat gcagcggtt 
      aaagctctc ctaaacgaa acttattca attacgaca 
      agtaatacg ccgcttaaa tcactcagt tcggagtta 
      actccagcc gaattgtcg gtcgttagt gtggccagc 
      gtttgggtg atcctcata ccgatgagt aagttcaaa 
      attgcgcct cagacaaga cttgcgcgc tctctggtg 
      aaagaaacg gttaataat gattga
    mRNA sequence
    • >TC033516 RA
      tatgtcaga tagtgtagt gtcagatag cctctacat 
      tattgtatc aataaatat ctaaaaagt aaatagcac 
      tccagagat aagataaga ggtaaacac tagtcaaaa 
      tgataataa atgaaatat taaaaaagt ttttcacag 
      gcaacaaaa cttagttta tcacggaat cgtgacgtt 
      taccttgtc caatgtaaa cgttctatt tagactttt 
      aaaaatacc agaaagggg ttttgaagt tcttggatt 
      tgttcttat acatataca agacttcgt tttgtgggg 
      caaatctag gcctgcttt aacccaagt ggtaacagc 
      tttccgttt atagatcgc cacatttat atgaagggg 
      gaaaacaag aaccgactc aatgtttca acacttgaa 
      gttattact atggaaaaa cttagtcat caggaagag 
      gcactggct tttaacatt aaccgaaat cttaattcc 
      cattttatt gttcacttt agtcgctta cggccgatc 
      taaaataag cggcttgaa caaacaagt catcgattt 
      tccaacgta ctatcgcga aaatacgct ggacgagat 
      ctactctca cttagatgt tggggaact gaaactgtg 
      gtattcgcc ctacaatga ttttactgg aaaaatcat 
      aagaaaagt ccaggaaac gttccaaat tcccggcga 
      tgatgtgag ggagttcac ttcggccga gaaactcga 
      gcagcattt tcagaccat ttctaactc ttccgcttt 
      gataacagt ggttaattt tcacggaaa cgaccccaa 
      acatctgga ttatgacct aagccttta cgataagta 
      cttattatg ggacaccga caagctttt cctccccgt 
      cataaacca ggaagaccg aagatcgtc aaaatacat 
      ctacaaggg cttcgctgc acttcaggt ggcaataga 
      cttggcatt tttggaact ccaaaccgg gcaaaaacc 
      aaaaatcga tttttccat tcaggaatt tccagaccc 
      gcctcacac tgacgattc gggcttgag ccaactttt 
      cttgtattt cctgccttt gataaccat cttcagctt 
      cattttttt gtgtccagc tgtgttgca acgagtggg 
      tgaagagaa acattcagg aactaagga gtttatgaa 
      aatggtcgg cctgaactc gtccatgtt atggctggg 
      ttggtttat tcatgcttt aataaccaa tctattctc 
      catcattgt tatcgtggt tttgatgaa agtaccttt 
      ctggggtgt tgcctatcc accgattga atattcaag 
      tggagtcgt tctcttcgt gtttttgtt cttgtactg 
      tatggctgc catcacgtt ctgtttcct cattgctac 
      cataatcag caaaccatc aattgcgac agtggtggg 
      cttactgat ttggatttt ttcgtactt cataccgca 
      agctttagt aacatcgca cgaaaacct ggcctggcg 
      gtttaaaat acctttggc gatcttccc taacatggc 
      attgcattt cggatacaa ctcaatttc agtttacga 
      aatgagaga gttaggcat ccaatggtc caacatttt 
      ccactcggg gagcggcgg tgaaaatga cgtaacaat 
      gggaaacgt tttcgtgat gttgattgt ggacatgat 
      tttcttcat gttattaac gatctacat cgaaaacgt 
      taaaccggg gaagtacgg aatcgccca gccttacac 
      tttcccgtt aaaatctct gatgaattg cgttagatg 
      ctttggttg ttttaccaa aattgacaa tgacccatc 
      cacaaaacg tttggatgc agagaatgg accaccagt 
      ttgtattga aatttgcga gctatttaa aaagttcaa 
      aaacgttgt tgccgttga aaacctcag tttgaacat 
      ttacaagaa tcacatcac agttttgtt gggtcataa 
      tggggctgg aaaaaccac aacaatgtc catcctcac 
      agggatgat caatgcgac aaatggtta tgttaaaat 
      caacggaaa agatatcaa gcaaaacac cagcgaggt 
      cagaagact gctaggtct ttgcccaca acacaatct 
      cctgtttcc cgacttgac cgtcttcga acatttgaa 
      atttttcgc aatgataaa gcggtcgaa aaactcaac 
      caaagaggc caaagaaat gttagaaca attgggtct 
      cgaggagaa aaaatacga tatggcttg tactttgtc 
      gggtgggat gaagcgcaa gctatgtct tggcatggc 
      gctaatcgg ggattcaca ggtattgat tttggacga 
      accgacgtc cggtatgga cccagagtc gcgtcgtaa 
      agtgtggaa catgttgct ggagtacag aaaatcaaa 
      gaccatcct aatcacgac ccattttat ggaggaagc 
      tgatgttct tggcgacta tatcgctat aatggctga 
      tggacggtt gcaagattc tgatacacc gtacaattt 
      gaaaaagaa atacaatac cggttacca catgtctct 
      aatgcttga gtcactcga ctttaagaa agaaataac 
      aggggaaat acggcgcca cattcctga agcacaatt 
      gattcgtac acatcatga cactctggt ttatttgtt 
      accgatgga taggaaatc taagtataa agatgtttt 
      tgtttcttt ggaagacaa aaaagctga ttggaaaat 
      tgttacgat cgggctcag tttgaccac acttaacga 
      tgtttttct aaaagccag ggacaaact tctgggaat 
      cgaacacag tgaaaccga cgggttaag caaaccaga 
      gtttagtac gcaaaaaac gaagaaatt gaatcgttc 
      gttgaacag ttttctggc gctgattgt caaacgttg 
      gtatttctt agagaaaaa attctattg gtactttcc 
      cgccgtgat tgtatcaat tacgatttt cctgctcgc 
      gatctggtt agggatctc aagcactga ctatagcaa 
      cgtcgctga gccccaatt gaacctaag ccttaaagt 
      ctacgacaa atcgtacgc ttatttcgg cggtgataa 
      tttaaccga gttggttaa aatgagaca gttttacga 
      gaaaatagt caaacaaca agacggtta tcccgaatt 
      gcataaagt cgtgtctga aggcatcat ccaagcagg 
      aacgaaaaa tatagcgtt ttacaacaa acacatgat 
      cgtggctgc agaatttaa tcaaagttc aaacgatga 
      gatcgttgt taatgccat gtacaatcg tttcgcgat 
      acatggagt tccaatttc cattaattt ggctatgaa 
      tgcagcggt taaagctct cctaaacga aacttattc 
      aattacgac aagtaatac gccgcttaa atcactcag 
      ttcggagtt aactccagc cgaattgtc ggtcgttag 
      tgtggccag cgtttgggt gatcctcat accgatgag 
      taagttcaa aattgcgcc tcagacaag acttgcgcg 
      ctctctggt gaaagaaac ggttaataa tgattgaaa 
      caagccact atggacttt ccccgagtc tagggcttt 
      tatttgctg atatccaga gattaaagt gaaaccaaa 
      aca
    • >TC033516 RB
      tatgtcaga tagtgtagt gtcagatag cctctacat 
      tattgtatc aataaatat ctaaaaagt aaatagcac 
      tccagagat aagataaga ggcaacaaa acttagttt 
      atcacggaa tcgtgacgt ttaccttgt ccaatgtaa 
      acgttctat ttagacttt taaaaatac cagaaaggg 
      gttttgaag ttcttggat ttgttctta tacatatac 
      aagacttcg ttttgtggg gcaaatcta ggcctgctt 
      taacccaag tggtaacag ctttccgtt tatagatcg 
      ccacattta tatgaaggg ggaaaacaa gttcgattt 
      gtgtttcag aaccgactc aatgtttca acacttgaa 
      gttattact atggaaaaa cttagtcat caggaagag 
      gcactggct tttaacatt aaccgaaat cttaattcc 
      cattttatt gttcacttt agtcgctta cggccgatc 
      taaaataag cggcttgaa caaacaagt catcgattt 
      tccaacgta ctatcgcga aaatacgct ggacgagat 
      ctactctca cttagatgt tggggaact gaaactgtg 
      gtattcgcc ctacaatga ttttactgg aaaaatcat 
      aagaaaagt ccaggaaac gttccaaat tcccggcga 
      tgatgtgag ggagttcac ttcggccga gaaactcga 
      gcagcattt tcagaccat ttctaactc ttccgcttt 
      gataacagt ggttaattt tcacggaaa cgaccccaa 
      acatctgga ttatgacct aagccttta cgataagta 
      cttattatg ggacaccga caagctttt cctccccgt 
      cataaacca ggaagaccg aagatcgtc aaaatacat 
      ctacaaggg cttcgctgc acttcaggt ggcaataga 
      cttggcatt tttggaact ccaaaccgg gcaaaaacc 
      aaaaatcga tttttccat tcaggaatt tccagaccc 
      gcctcacac tgacgattc gggcttgag ccaactttt 
      cttgtattt cctgccttt gataaccat cttcagctt 
      cattttttt gtgtccagc tgtgttgca acgagtggg 
      tgaagagaa acattcagg aactaagga gtttatgaa 
      aatggtcgg cctgaactc gtccatgtt atggctggg 
      ttggtttat tcatgcttt aataaccaa tctattctc 
      catcattgt tatcgtggt tttgatgaa agtaccttt 
      ctggggtgt tgcctatcc accgattga atattcaag 
      tggagtcgt tctcttcgt gtttttgtt cttgtactg 
      tatggctgc catcacgtt ctgtttcct cattgctac 
      cataatcag caaaccatc aattgcgac agtggtggg 
      cttactgat ttggatttt ttcgtactt cataccgca 
      agctttagt aacatcgca cgaaaacct ggcctggcg 
      gtttaaaat acctttggc gatcttccc taacatggc 
      attgcattt cggatacaa ctcaatttc agtttacga 
      aatgagaga gttaggcat ccaatggtc caacatttt 
      ccactcggg gagcggcgg tgaaaatga cgtaacaat 
      gggaaacgt tttcgtgat gttgattgt ggacatgat 
      tttcttcat gttattaac gatctacat cgaaaacgt 
      taaaccggg gaagtacgg aatcgccca gccttacac 
      tttcccgtt aaaatctct gatgaattg cgttagatg 
      ctttggttg ttttaccaa aattgacaa tgacccatc 
      cacaaaacg tttggatgc agagaatgg accaccagt 
      ttgtattga aatttgcga gctatttaa aaagttcaa 
      aaacgttgt tgccgttga aaacctcag tttgaacat 
      ttacaagaa tcacatcac agttttgtt gggtcataa 
      tggggctgg aaaaaccac aacaatgtc catcctcac 
      agggatgat caatgcgac aaatggtta tgttaaaat 
      caacggaaa agatatcaa gcaaaacac cagcgaggt 
      cagaagact gctaggtct ttgcccaca acacaatct 
      cctgtttcc cgacttgac cgtcttcga acatttgaa 
      atttttcgc aatgataaa gcggtcgaa aaactcaac 
      caaagaggc caaagaaat gttagaaca attgggtct 
      cgaggagaa aaaatacga tatggcttg tactttgtc 
      gggtgggat gaagcgcaa gctatgtct tggcatggc 
      gctaatcgg ggattcaca ggtattgat tttggacga 
      accgacgtc cggtatgga cccagagtc gcgtcgtaa 
      agtgtggaa catgttgct ggagtacag aaaatcaaa 
      gaccatcct aatcacgac ccattttat ggaggaagc 
      tgatgttct tggcgacta tatcgctat aatggctga 
      tggacggtt gcaagattc tgatacacc gtacaattt 
      gaaaaagaa atacaatac cggttacca catgtctct 
      aatgcttga gtcactcga ctttaagaa agaaataac 
      aggggaaat acggcgcca cattcctga agcacaatt 
      gattcgtac acatcatga cactctggt ttatttgtt 
      accgatgga taggaaatc taagtataa agatgtttt 
      tgtttcttt ggaagacaa aaaagctga ttggaaaat 
      tgttacgat cgggctcag tttgaccac acttaacga 
      tgtttttct aaaagccag ggacaaact tctgggaat 
      cgaacacag tgaaaccga cgggttaag caaaccaga 
      gtttagtac gcaaaaaac gaagaaatt gaatcgttc 
      gttgaacag ttttctggc gctgattgt caaacgttg 
      gtatttctt agagaaaaa attctattg gtactttcc 
      cgccgtgat tgtatcaat tacgatttt cctgctcgc 
      gatctggtt agggatctc aagcactga ctatagcaa 
      cgtcgctga gccccaatt gaacctaag ccttaaagt 
      ctacgacaa atcgtacgc ttatttcgg cggtgataa 
      tttaaccga gttggttaa aatgagaca gttttacga 
      gaaaatagt caaacaaca agacggtta tcccgaatt 
      gcataaagt cgtgtctga aggcatcat ccaagcagg 
      aacgaaaaa tatagcgtt ttacaacaa acacatgat 
      cgtggctgc agaatttaa tcaaagttc aaacgatga 
      gatcgttgt taatgccat gtacaatcg tttcgcgat 
      acatggagt tccaatttc cattaattt ggctatgaa 
      tgcagcggt taaagctct cctaaacga aacttattc 
      aattacgac aagtaatac gccgcttaa atcactcag 
      ttcggagtt aactccagc cgaattgtc ggtcgttag 
      tgtggccag cgtttgggt gatcctcat accgatgag 
      taagttcaa aattgcgcc tcagacaag acttgcgcg 
      ctctctggt gaaagaaac ggttaataa tgattgaaa 
      caagccact atggacttt ccccgagtc tagggcttt 
      tatttgctg atatccaga gattaaagt gaaaccaaa 
      aca