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Gene information
General information
TC015779 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 2717526..2718590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015779
    iBeetle screen iB_02827
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015779 RA
      MRFLNLIFI VCFTLTPII WAKAPGPRI INGKTAEKG 
      QFPWQVAIH VTQPGVSTL CGGALLNEK WILTAGHCV 
      KDATNFKIA VGSNHFNGD DPSRVVFQT SDYILHEDY 
      NKYTLANDI GLIPLPQAV SFNDDIQPI ALPSQGLTD 
      GSTVTVSGW GLTSDDGEE ASPELMYVD LVTISNSEC 
      STAYDGLDI NNGVVCAKG PGTIVQSTC EGDSGGPLV 
      TRDSNPTHV GIVSFGHPD GCESGKPAG FTRTYNYID 
      WIKGKTGI
    CDS sequence
    • >TC015779 RA
      atgcgtttt ctcaattta atttttatt gtgtgtttt 
      actcttacc ccaataatt tgggcaaaa gcgccaggt 
      ccacgaatt ataaatggc aaaacggcc gaaaaaggt 
      caatttccg tggcaagtg gcgattcat gtcacccaa 
      cctggagtt tcaactttg tgtggaggg gctctcttg 
      aacgaaaaa tggattcta acggccggt cattgcgtc 
      aaagacgcc actaatttt aaaattgct gtgggctca 
      aaccacttc aatggagat gatccctca cgggttgta 
      ttccagaca agtgattac attttgcac gaagattac 
      aacaagtat actctagct aacgatatt ggacttata 
      ccattacca caagctgta tcattcaac gacgacatt 
      caaccaatt gccctgcca tcacaggga ctaactgat 
      ggtagtacg gtcaccgtc agtggctgg ggcctaacc 
      agtgatgac ggcgaagaa gccagtcct gaactaatg 
      tacgttgat ttagtcaca atttccaac tcggaatgt 
      tccacggcc tatgacgga cttgacata aataacgga 
      gttgtttgt gccaaaggg cccggaact atcgtccag 
      agcacttgc gagggtgac agtggcggt ccgctcgtt 
      acacgcgat tcaaacccc actcatgtt ggtattgtt 
      agttttgga caccctgac ggttgtgag agtggcaaa 
      cctgccggt ttcacgcgc acttataac tacattgat 
      tggattaaa ggaaaaact ggtatttga
    mRNA sequence
    • >TC015779 RA
      cttattttg agataaaat gcgttttct caatttaat 
      ttttattgt gtgttttac tcttacccc aataatttg 
      ggcaaaagc gccaggtcc acgaattat aaatggcaa 
      aacggccga aaaaggtca atttccgtg gcaagtggc 
      gattcatgt cacccaacc tggagtttc aactttgtg 
      tggaggggc tctcttgaa cgaaaaatg gattctaac 
      ggccggtca ttgcgtcaa agacgccac taattttaa 
      aattgctgt gggctcaaa ccacttcaa tggagatga 
      tccctcacg ggttgtatt ccagacaag tgattacat 
      tttgcacga agattacaa caagtatac tctagctaa 
      cgatattgg acttatacc attaccaca agctgtatc 
      attcaacga cgacattca accaattgc cctgccatc 
      acagggact aactgatgg tagtacggt caccgtcag 
      tggctgggg cctaaccag tgatgacgg cgaagaagc 
      cagtcctga actaatgta cgttgattt agtcacaat 
      ttccaactc ggaatgttc cacggccta tgacggact 
      tgacataaa taacggagt tgtttgtgc caaagggcc 
      cggaactat cgtccagag cacttgcga gggtgacag 
      tggcggtcc gctcgttac acgcgattc aaaccccac 
      tcatgttgg tattgttag ttttggaca ccctgacgg 
      ttgtgagag tggcaaacc tgccggttt cacgcgcac 
      ttataacta cattgattg gattaaagg aaaaactgg 
      tatttgaaa acaataaac aagtcgtat ttta