Status
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Gene information
General information
TC033441
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015772
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7934521..7944425
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033441
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02826

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033441 RA
      MKCEEEGKK WPQFLVVFA ATFVYLGTG VHTGWPAPS 
      LPQLLSEAY PHKVTNDEA SYITIIGHL GNICGGFLG 
      NLLLDKIGR KKTILLISL PQIVSFLLI IASYEVMEL 
      LYLGRFIGG VAEGATFSF MPVYIAEVA QPEIRGSLG 
      VFMSVMCVV GMLLANVIG SALTIKQSA EVYLIFPII 
      FVTVFYKMP ESPYYLLMK NRKLEAESV LKFLRRKKS 
      VSEELVKLT NDVNQQMSE SGTFRDIFT IESNRKALL 
      LVIVLRIFQ QCTGVSAFS LYIQILLNE ATQILAPHV 
      GASILLLIQ VLMSVLSSF FIDKWGRKP LLIFSSIGC 
      FITLAFQTI FFAFKEYSN VDVSLVDWF PLILMRDVM 
      TLASQEEGK KWPQFLAVF AATFVYLGT GVHTGWPAP 
      SLPQLLSEA YPHKVTNDE ASYITIIGH LGNICGGFL 
      GNLLLDKIG RKKTILLIS LPQILSFLL IIASYEVME 
      LLYLGRFIG GVAEGATFS FMPVYIAEV AQPEIRGSL 
      GTLMSVMRV SGMLLVNLI GSYLTIKQS AMIFLLFPI 
      IFVTVFYKM PESPYYLLM KNRKLEAES VLKFLRRKK 
      SVSEELVKL TNDVNRQMS ESGTFRDIF RIESNRKAL 
      FLVGLLRIF QQCTGFSAF SSYVQILLS EATQTLAPH 
      IGASILLLV QLFMAVLSS FFVDKWGRK PLLIFSTIG 
      CFINLTLQT IFFAMKEYT NFEVSVIDW FPLVMMIIF 
      MILYFSGLG VTVNIVTSE MFSASVKGK TISLVNATF 
      AFGMLATTK FYQTTADNF GLTVPFSIF ALLTLFAVI 
      FDNFAHCNI DELKPHTFH QLTHIHDLH LDNNDLKTF 
      PSDLFPESN NLRLLSVTH NHISRISSD AFRKLRALE 
      ELDLSGNKI SEIKREVLA PLAKLRLLI LRNNQIRYI 
      SNSTFPQLP LRKLSLIEN RIDRLDPGA FANLTQLQE 
      L
    CDS sequence
    • >TC033441 RA
      atgaaatgt gaagaagag ggcaaaaag tggccacag 
      ttccttgta gttttcgct gcaactttt gtctatctc 
      ggaactggg gttcacact ggatggcca gctccctcc 
      ctcccccag ctcctctcc gaggcatac ccccataaa 
      gtaaccaat gacgaagcc tcctatatc acaatcatc 
      ggccatttg ggcaacatt tgtggcggt tttttggga 
      aatctcctc cttgataaa atcgggcgc aaaaaaaca 
      atcctccta atctccctc ccccaaatt gtgtcattc 
      ctcctaatt attgcctcc tacgaagta atggagttg 
      ttatacttg ggccgtttc attggggga gttgccgaa 
      ggagcgact ttttccttc atgcctgtc tacattgcc 
      gaagttgca caaccggaa atcagaggg agtttgggc 
      gttttcatg tcggttatg tgtgtcgtt ggaatgctc 
      ctcgccaac gtgattggt tccgctttg accattaaa 
      caatcagcg gaagtttac ctaattttc ccgatcatt 
      tttgtaaca gttttctac aaaatgccc gaaagtccc 
      tactatctt ttgatgaag aaccgaaaa cttgaagca 
      gagtcggtt ttgaaattt ttgcgccgg aaaaaaagt 
      gtttcagaa gagttggta aaactgacg aatgatgtg 
      aaccaacaa atgtccgag tccgggacg ttccgggac 
      attttcacg atcgagtcc aaccggaaa gctttgcta 
      cttgtgatc gtgctacga atcttccag caatgcacc 
      ggagtttcg gcttttagt ctctacatt cagatactt 
      ttgaacgaa gcgacgcaa attttggcc cctcatgtc 
      ggggcttcg attcttctc ctgattcag gttttaatg 
      tcggttttg tcctcgttt tttatcgat aaatgggga 
      cggaaacct ctactgatt ttttcatca attggttgt 
      ttcatcaca ctagctttt cagacgatt tttttcgcc 
      tttaaagag tactcaaat gttgacgtt tcgctcgtt 
      gattggttt cccttaatt ttgatgcgt gatgtgatg 
      actttggca agtcaagaa gaaggcaaa aaatggcca 
      caattcctt gcagttttt gccgcaact tttgtctat 
      ctcggaact ggggttcac actggatgg ccagctccc 
      tccctcccc cagcttctc tccgaggca tacccccat 
      aaagtaacc aatgacgaa gcctcctac atcacaatc 
      atcggccat ttgggcaac atttgtggc ggttttttg 
      ggaaatctc ctccttgat aaaatcggg cgcaaaaaa 
      acaatcctc ctaatctcc ctcccccaa attttgtca 
      ttcctccta attattgcc tcctacgaa gtaatggag 
      ttgttatac ttgggccgc ttcattggg ggagttgcc 
      gaaggagcg actttttcc ttcatgcct gtctacatt 
      gctgaagtt gcacaaccg gaaatcagg gggagttta 
      gggaccctc atgtccgta atgcgtgtc tctggaatg 
      ctcctcgtc aatttgatt ggttcatat ttgaccatc 
      aaacaatcg gcaatgatt ttcctactt ttcccgatc 
      atttttgta acagttttc tacaaaatg cccgaaagt 
      ccctactat ctcttgatg aagaaccga aaacttgaa 
      gcagagtcg gttttgaaa tttttgcgc cggaaaaaa 
      agtgtttca gaagagttg gtaaaactg acgaatgat 
      gtgaaccga caaatgtca gaatccggg acgttccgg 
      gacattttc aggatcgag tccaaccgg aaagccctt 
      tttctcgta ggtttgtta cgaattttc cagcaatgt 
      accggattt tcggcgttt agttcctac gtccagatt 
      cttttaagc gaagcgacc caaactttg gcccctcat 
      attggagcc tcaatcctc ctactagtc cagcttttt 
      atggccgtt ttatcgtca tttttcgtc gataaatgg 
      ggccggaaa ccactatta attttttca acgattggt 
      tgtttcatt aacttaacc ctccagacg atttttttc 
      gcaatgaaa gagtacaca aatttcgag gtttcagtt 
      attgattgg tttcctctt gttatgatg atcatattt 
      atgatttta tatttctcg ggtttaggg gtcactgtt 
      aacattgtt acaagcgaa atgttttca gcaagtgtg 
      aaaggtaaa acaatcagc ttggttaat gcaactttt 
      gcgttcggt atgttggcc acaacgaaa ttctaccaa 
      acaaccgcc gataatttc ggtttgacc gttcctttc 
      tctattttc gctcttctg acactgttc gctgttatt 
      tttgacaac tttgctcac tgcaacatc gacgagcta 
      aaaccgcac acatttcac caactgacc cacatccat 
      gatttacac ttagacaac aacgatctc aaaacgttt 
      ccttccgac ttgttcccc gaaagcaac aatttacgt 
      ctgttatcc gtaacgcac aatcatata tcgcggatt 
      tcgagcgat gcttttagg aaattgcga gcgctggaa 
      gaactggat ctgagtggg aacaaaatc agcgaaatc 
      aagagagaa gtgctagcg ccgcttgca aaactgcgc 
      cttttaatt ctgaggaat aaccaaata cgatacatc 
      agcaattcc acctttcca caactgcct ttgcgcaaa 
      ttatccttg atagaaaac aggatcgat cgcttggac 
      ccgggggct ttcgcaaat ttgacacaa ctccaagaa 
      ttgtaa
    mRNA sequence
    • >TC033441 RA
      aaacatgaa aatgaaatg tgaagaaga gggcaaaaa 
      gtggccaca gttccttgt agttttcgc tgcaacttt 
      tgtctatct cggaactgg ggttcacac tggatggcc 
      agctccctc cctccccca gctcctctc cgaggcata 
      cccccataa agtaaccaa tgacgaagc ctcctatat 
      cacaatcat cggccattt gggcaacat ttgtggcgg 
      ttttttggg aaatctcct ccttgataa aatcgggcg 
      caaaaaaac aatcctcct aatctccct cccccaaat 
      tgtgtcatt cctcctaat tattgcctc ctacgaagt 
      aatggagtt gttatactt gggccgttt cattggggg 
      agttgccga aggagcgac tttttcctt catgcctgt 
      ctacattgc cgaagttgc acaaccgga aatcagagg 
      gagtttggg cgttttcat gtcggttat gtgtgtcgt 
      tggaatgct cctcgccaa cgtgattgg ttccgcttt 
      gaccattaa acaatcagc ggaagttta cctaatttt 
      cccgatcat ttttgtaac agttttcta caaaatgcc 
      cgaaagtcc ctactatct tttgatgaa gaaccgaaa 
      acttgaagc agagtcggt tttgaaatt tttgcgccg 
      gaaaaaaag tgtttcaga agagttggt aaaactgac 
      gaatgatgt gaaccaaca aatgtccga gtccgggac 
      gttccggga cattttcac gatcgagtc caaccggaa 
      agctttgct acttgtgat cgtgctacg aatcttcca 
      gcaatgcac cggagtttc ggcttttag tctctacat 
      tcagatact tttgaacga agcgacgca aattttggc 
      ccctcatgt cggggcttc gattcttct cctgattca 
      ggttttaat gtcggtttt gtcctcgtt ttttatcga 
      taaatgggg acggaaacc tctactgat tttttcatc 
      aattggttg tttcatcac actagcttt tcagacgat 
      ttttttcgc ctttaaaga gtactcaaa tgttgacgt 
      ttcgctcgt tgattggtt tcccttaat tttgatgcg 
      tgatgtgat gactttggc aagtcaaga agaaggcaa 
      aaaatggcc acaattcct tgcagtttt tgccgcaac 
      ttttgtcta tctcggaac tggggttca cactggatg 
      gccagctcc ctccctccc ccagcttct ctccgaggc 
      ataccccca taaagtaac caatgacga agcctccta 
      catcacaat catcggcca tttgggcaa catttgtgg 
      cggtttttt gggaaatct cctccttga taaaatcgg 
      gcgcaaaaa aacaatcct cctaatctc cctccccca 
      aattttgtc attcctcct aattattgc ctcctacga 
      agtaatgga gttgttata cttgggccg cttcattgg 
      gggagttgc cgaaggagc gactttttc cttcatgcc 
      tgtctacat tgctgaagt tgcacaacc ggaaatcag 
      ggggagttt agggaccct catgtccgt aatgcgtgt 
      ctctggaat gctcctcgt caatttgat tggttcata 
      tttgaccat caaacaatc ggcaatgat tttcctact 
      tttcccgat catttttgt aacagtttt ctacaaaat 
      gcccgaaag tccctacta tctcttgat gaagaaccg 
      aaaacttga agcagagtc ggttttgaa atttttgcg 
      ccggaaaaa aagtgtttc agaagagtt ggtaaaact 
      gacgaatga tgtgaaccg acaaatgtc agaatccgg 
      gacgttccg ggacatttt caggatcga gtccaaccg 
      gaaagccct ttttctcgt aggtttgtt acgaatttt 
      ccagcaatg taccggatt ttcggcgtt tagttccta 
      cgtccagat tcttttaag cgaagcgac ccaaacttt 
      ggcccctca tattggagc ctcaatcct cctactagt 
      ccagctttt tatggccgt tttatcgtc atttttcgt 
      cgataaatg gggccggaa accactatt aattttttc 
      aacgattgg ttgtttcat taacttaac cctccagac 
      gattttttt cgcaatgaa agagtacac aaatttcga 
      ggtttcagt tattgattg gtttcctct tgttatgat 
      gatcatatt tatgatttt atatttctc gggtttagg 
      ggtcactgt taacattgt tacaagcga aatgttttc 
      agcaagtgt gaaaggtaa aacaatcag cttggttaa 
      tgcaacttt tgcgttcgg tatgttggc cacaacgaa 
      attctacca aacaaccgc cgataattt cggtttgac 
      cgttccttt ctctatttt cgctcttct gacactgtt 
      cgctgttat ttttgacaa ctttgctca ctgcaacat 
      cgacgagct aaaaccgca cacatttca ccaactgac 
      ccacatcca tgatttaca cttagacaa caacgatct 
      caaaacgtt tccttccga cttgttccc cgaaagcaa 
      caatttacg tctgttatc cgtaacgca caatcatat 
      atcgcggat ttcgagcga tgcttttag gaaattgcg 
      agcgctgga agaactgga tctgagtgg gaacaaaat 
      cagcgaaat caagagaga agtgctagc gccgcttgc 
      aaaactgcg ccttttaat tctgaggaa taaccaaat 
      acgatacat cagcaattc cacctttcc acaactgcc 
      tttgcgcaa attatcctt gatagaaaa caggatcga 
      tcgcttgga cccgggggc tttcgcaaa tttgacaca 
      actccaaga attgtaagt tggaattta tttcacagt 
      gataagtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY