| General information | |||
| TC033441
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 7934521..7944425 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033441 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02826 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC033441 RA
MKCEEEGKK WPQFLVVFA ATFVYLGTG VHTGWPAPS LPQLLSEAY PHKVTNDEA SYITIIGHL GNICGGFLG NLLLDKIGR KKTILLISL PQIVSFLLI IASYEVMEL LYLGRFIGG VAEGATFSF MPVYIAEVA QPEIRGSLG VFMSVMCVV GMLLANVIG SALTIKQSA EVYLIFPII FVTVFYKMP ESPYYLLMK NRKLEAESV LKFLRRKKS VSEELVKLT NDVNQQMSE SGTFRDIFT IESNRKALL LVIVLRIFQ QCTGVSAFS LYIQILLNE ATQILAPHV GASILLLIQ VLMSVLSSF FIDKWGRKP LLIFSSIGC FITLAFQTI FFAFKEYSN VDVSLVDWF PLILMRDVM TLASQEEGK KWPQFLAVF AATFVYLGT GVHTGWPAP SLPQLLSEA YPHKVTNDE ASYITIIGH LGNICGGFL GNLLLDKIG RKKTILLIS LPQILSFLL IIASYEVME LLYLGRFIG GVAEGATFS FMPVYIAEV AQPEIRGSL GTLMSVMRV SGMLLVNLI GSYLTIKQS AMIFLLFPI IFVTVFYKM PESPYYLLM KNRKLEAES VLKFLRRKK SVSEELVKL TNDVNRQMS ESGTFRDIF RIESNRKAL FLVGLLRIF QQCTGFSAF SSYVQILLS EATQTLAPH IGASILLLV QLFMAVLSS FFVDKWGRK PLLIFSTIG CFINLTLQT IFFAMKEYT NFEVSVIDW FPLVMMIIF MILYFSGLG VTVNIVTSE MFSASVKGK TISLVNATF AFGMLATTK FYQTTADNF GLTVPFSIF ALLTLFAVI FDNFAHCNI DELKPHTFH QLTHIHDLH LDNNDLKTF PSDLFPESN NLRLLSVTH NHISRISSD AFRKLRALE ELDLSGNKI SEIKREVLA PLAKLRLLI LRNNQIRYI SNSTFPQLP LRKLSLIEN RIDRLDPGA FANLTQLQE L
- >TC033441 RA
atgaaatgt gaagaagag ggcaaaaag tggccacag ttccttgta gttttcgct gcaactttt gtctatctc ggaactggg gttcacact ggatggcca gctccctcc ctcccccag ctcctctcc gaggcatac ccccataaa gtaaccaat gacgaagcc tcctatatc acaatcatc ggccatttg ggcaacatt tgtggcggt tttttggga aatctcctc cttgataaa atcgggcgc aaaaaaaca atcctccta atctccctc ccccaaatt gtgtcattc ctcctaatt attgcctcc tacgaagta atggagttg ttatacttg ggccgtttc attggggga gttgccgaa ggagcgact ttttccttc atgcctgtc tacattgcc gaagttgca caaccggaa atcagaggg agtttgggc gttttcatg tcggttatg tgtgtcgtt ggaatgctc ctcgccaac gtgattggt tccgctttg accattaaa caatcagcg gaagtttac ctaattttc ccgatcatt tttgtaaca gttttctac aaaatgccc gaaagtccc tactatctt ttgatgaag aaccgaaaa cttgaagca gagtcggtt ttgaaattt ttgcgccgg aaaaaaagt gtttcagaa gagttggta aaactgacg aatgatgtg aaccaacaa atgtccgag tccgggacg ttccgggac attttcacg atcgagtcc aaccggaaa gctttgcta cttgtgatc gtgctacga atcttccag caatgcacc ggagtttcg gcttttagt ctctacatt cagatactt ttgaacgaa gcgacgcaa attttggcc cctcatgtc ggggcttcg attcttctc ctgattcag gttttaatg tcggttttg tcctcgttt tttatcgat aaatgggga cggaaacct ctactgatt ttttcatca attggttgt ttcatcaca ctagctttt cagacgatt tttttcgcc tttaaagag tactcaaat gttgacgtt tcgctcgtt gattggttt cccttaatt ttgatgcgt gatgtgatg actttggca agtcaagaa gaaggcaaa aaatggcca caattcctt gcagttttt gccgcaact tttgtctat ctcggaact ggggttcac actggatgg ccagctccc tccctcccc cagcttctc tccgaggca tacccccat aaagtaacc aatgacgaa gcctcctac atcacaatc atcggccat ttgggcaac atttgtggc ggttttttg ggaaatctc ctccttgat aaaatcggg cgcaaaaaa acaatcctc ctaatctcc ctcccccaa attttgtca ttcctccta attattgcc tcctacgaa gtaatggag ttgttatac ttgggccgc ttcattggg ggagttgcc gaaggagcg actttttcc ttcatgcct gtctacatt gctgaagtt gcacaaccg gaaatcagg gggagttta gggaccctc atgtccgta atgcgtgtc tctggaatg ctcctcgtc aatttgatt ggttcatat ttgaccatc aaacaatcg gcaatgatt ttcctactt ttcccgatc atttttgta acagttttc tacaaaatg cccgaaagt ccctactat ctcttgatg aagaaccga aaacttgaa gcagagtcg gttttgaaa tttttgcgc cggaaaaaa agtgtttca gaagagttg gtaaaactg acgaatgat gtgaaccga caaatgtca gaatccggg acgttccgg gacattttc aggatcgag tccaaccgg aaagccctt tttctcgta ggtttgtta cgaattttc cagcaatgt accggattt tcggcgttt agttcctac gtccagatt cttttaagc gaagcgacc caaactttg gcccctcat attggagcc tcaatcctc ctactagtc cagcttttt atggccgtt ttatcgtca tttttcgtc gataaatgg ggccggaaa ccactatta attttttca acgattggt tgtttcatt aacttaacc ctccagacg atttttttc gcaatgaaa gagtacaca aatttcgag gtttcagtt attgattgg tttcctctt gttatgatg atcatattt atgatttta tatttctcg ggtttaggg gtcactgtt aacattgtt acaagcgaa atgttttca gcaagtgtg aaaggtaaa acaatcagc ttggttaat gcaactttt gcgttcggt atgttggcc acaacgaaa ttctaccaa acaaccgcc gataatttc ggtttgacc gttcctttc tctattttc gctcttctg acactgttc gctgttatt tttgacaac tttgctcac tgcaacatc gacgagcta aaaccgcac acatttcac caactgacc cacatccat gatttacac ttagacaac aacgatctc aaaacgttt ccttccgac ttgttcccc gaaagcaac aatttacgt ctgttatcc gtaacgcac aatcatata tcgcggatt tcgagcgat gcttttagg aaattgcga gcgctggaa gaactggat ctgagtggg aacaaaatc agcgaaatc aagagagaa gtgctagcg ccgcttgca aaactgcgc cttttaatt ctgaggaat aaccaaata cgatacatc agcaattcc acctttcca caactgcct ttgcgcaaa ttatccttg atagaaaac aggatcgat cgcttggac ccgggggct ttcgcaaat ttgacacaa ctccaagaa ttgtaa
- >TC033441 RA
aaacatgaa aatgaaatg tgaagaaga gggcaaaaa gtggccaca gttccttgt agttttcgc tgcaacttt tgtctatct cggaactgg ggttcacac tggatggcc agctccctc cctccccca gctcctctc cgaggcata cccccataa agtaaccaa tgacgaagc ctcctatat cacaatcat cggccattt gggcaacat ttgtggcgg ttttttggg aaatctcct ccttgataa aatcgggcg caaaaaaac aatcctcct aatctccct cccccaaat tgtgtcatt cctcctaat tattgcctc ctacgaagt aatggagtt gttatactt gggccgttt cattggggg agttgccga aggagcgac tttttcctt catgcctgt ctacattgc cgaagttgc acaaccgga aatcagagg gagtttggg cgttttcat gtcggttat gtgtgtcgt tggaatgct cctcgccaa cgtgattgg ttccgcttt gaccattaa acaatcagc ggaagttta cctaatttt cccgatcat ttttgtaac agttttcta caaaatgcc cgaaagtcc ctactatct tttgatgaa gaaccgaaa acttgaagc agagtcggt tttgaaatt tttgcgccg gaaaaaaag tgtttcaga agagttggt aaaactgac gaatgatgt gaaccaaca aatgtccga gtccgggac gttccggga cattttcac gatcgagtc caaccggaa agctttgct acttgtgat cgtgctacg aatcttcca gcaatgcac cggagtttc ggcttttag tctctacat tcagatact tttgaacga agcgacgca aattttggc ccctcatgt cggggcttc gattcttct cctgattca ggttttaat gtcggtttt gtcctcgtt ttttatcga taaatgggg acggaaacc tctactgat tttttcatc aattggttg tttcatcac actagcttt tcagacgat ttttttcgc ctttaaaga gtactcaaa tgttgacgt ttcgctcgt tgattggtt tcccttaat tttgatgcg tgatgtgat gactttggc aagtcaaga agaaggcaa aaaatggcc acaattcct tgcagtttt tgccgcaac ttttgtcta tctcggaac tggggttca cactggatg gccagctcc ctccctccc ccagcttct ctccgaggc ataccccca taaagtaac caatgacga agcctccta catcacaat catcggcca tttgggcaa catttgtgg cggtttttt gggaaatct cctccttga taaaatcgg gcgcaaaaa aacaatcct cctaatctc cctccccca aattttgtc attcctcct aattattgc ctcctacga agtaatgga gttgttata cttgggccg cttcattgg gggagttgc cgaaggagc gactttttc cttcatgcc tgtctacat tgctgaagt tgcacaacc ggaaatcag ggggagttt agggaccct catgtccgt aatgcgtgt ctctggaat gctcctcgt caatttgat tggttcata tttgaccat caaacaatc ggcaatgat tttcctact tttcccgat catttttgt aacagtttt ctacaaaat gcccgaaag tccctacta tctcttgat gaagaaccg aaaacttga agcagagtc ggttttgaa atttttgcg ccggaaaaa aagtgtttc agaagagtt ggtaaaact gacgaatga tgtgaaccg acaaatgtc agaatccgg gacgttccg ggacatttt caggatcga gtccaaccg gaaagccct ttttctcgt aggtttgtt acgaatttt ccagcaatg taccggatt ttcggcgtt tagttccta cgtccagat tcttttaag cgaagcgac ccaaacttt ggcccctca tattggagc ctcaatcct cctactagt ccagctttt tatggccgt tttatcgtc atttttcgt cgataaatg gggccggaa accactatt aattttttc aacgattgg ttgtttcat taacttaac cctccagac gattttttt cgcaatgaa agagtacac aaatttcga ggtttcagt tattgattg gtttcctct tgttatgat gatcatatt tatgatttt atatttctc gggtttagg ggtcactgt taacattgt tacaagcga aatgttttc agcaagtgt gaaaggtaa aacaatcag cttggttaa tgcaacttt tgcgttcgg tatgttggc cacaacgaa attctacca aacaaccgc cgataattt cggtttgac cgttccttt ctctatttt cgctcttct gacactgtt cgctgttat ttttgacaa ctttgctca ctgcaacat cgacgagct aaaaccgca cacatttca ccaactgac ccacatcca tgatttaca cttagacaa caacgatct caaaacgtt tccttccga cttgttccc cgaaagcaa caatttacg tctgttatc cgtaacgca caatcatat atcgcggat ttcgagcga tgcttttag gaaattgcg agcgctgga agaactgga tctgagtgg gaacaaaat cagcgaaat caagagaga agtgctagc gccgcttgc aaaactgcg ccttttaat tctgaggaa taaccaaat acgatacat cagcaattc cacctttcc acaactgcc tttgcgcaa attatcctt gatagaaaa caggatcga tcgcttgga cccgggggc tttcgcaaa tttgacaca actccaaga attgtaagt tggaattta tttcacagt gataagtaa
