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Gene information
General information
TC015747 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7365529..7391113
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015747
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02824

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015747 RA
      MITIPNYGY NPPVRISSH SDASYIEKK TVSELYKQM 
KDQNQAPTR SVSSAANLT SLCVDISPS SSNFFEVLY 
IGKIKVWQK KVPDTFIDD ALEKFKTHE MEKIKAKML 
GNIGRLRSE DNIRRGSNS STSSVESYG HPHLQRSQS 
SMSGLTVKT VEDKDEDKE DDSKSVDMD DHNRTMVLQ 
VDRTDLRLI SPDRKVILL HKQHKDITT CIQGVQKME 
HFGFVCREG KNGQTFVGY IFKCESSSI ATDAVSAIT 
QAFASSESR PRPAITSCD HCPMVWFHK LCTEIERMS 
DRKTQAAIL RRLEELDLE EQNTILAKF KGAETDSVR 
EQNEFLMML LRAHCEMKQ GRHVHDTAE NRSEFLNQY 
LGGGGTIFM KAKRSLTNS FEMLKRKAS KDDFGASLR 
EMNRENSPH SPTIEVSDK DSEGMRSRS STIGSQSDL 
HLMVQETKS SVSPDRSVT TPDRTPKSP MMDIFLKVG 
SSPKSVTSE DGSPKIDSG SWRQAIFKR VITPSKNDK 
PVEMKQRTK EELRELWKK SIYQAILLV RMEKENARL 
KAEQEESAV KRIKLEYDE MKPPQREVM EVWEMLTNK 
EARMKCDNQ MLLQAIRQG VPRGKRGEV WQFLAEQYC 
MRTAPIDTS NFPNYNVPY EQLLKQLTS HQHAILIDL 
GRTFPNHSY FSSPLGPGQ LALFNLLKA YSLLDSEMG 
YCQGMSFVA GVLLLHMEE SQAFFLLRH LMFRRGLRL 
QYLPDMVGL QVKLYQLSR LLHDQLPDL YNHFDFYEV 
APTLYAAPW LLTIFASQF PLGFVTRVF DLIFLESPD 
VIFRVAIAL LTFHKDNLL ACDSFEEIM NYLKNKLPT 
IDKPTLDKI MKQVYTTDI SKQLNEYKV EYQVLQEEM 
TSVQPQVEA LHKLESQNK TLTEQNKAL MAQLELALS 
NVQRLEKTR TLQQAQLNK LEMHSRALD VTIATLGNF 
LNTLIEQKV EIEIPDDVR RILNQISYS EKRKSELKP 
QNNLMKMFQ KSENDKALV KSLSTGKIM LPVTLDQNI 
LRSNSLEKS PQKTSQFFS SSHNHILQQ FNNNNKIDI 
KVQECDLNK SISLPLDSY HEKVSPCNS VDSGVGTPS 
SPKPGDHHP LSNCDVNFT FHGTRELKN IKSIRNMSR 
NSSPDIIGK
    CDS sequence
    • >TC015747 RA
      atgataacg ataccaaac tacggttac aatccacct 
gtgcgtatt tcttcgcat agtgatgcc tcctacatt 
gaaaagaaa accgtttca gaattgtat aaacaaatg 
aaggaccaa aatcaggca ccgacacgt tctgtgagc 
agtgctgcc aatttaaca tcactatgt gtggacata 
tcgcccagt tctagcaat ttcttcgag gttttatac 
attgggaaa ataaaagtt tggcaaaag aaagtccct 
gatacgttc attgatgat gctttggag aaattcaaa 
acacatgaa atggaaaaa atcaaggcg aaaatgttg 
ggcaatatc ggacgattg agaagtgaa gataacatt 
cgaagagga tcgaatagt tcgactagt tcggttgaa 
agttacggg catcctcat ttgcaaaga tcgcaatct 
tccatgagt ggacttaca gtgaagact gttgaagat 
aaagacgaa gataaggaa gatgattca aagagtgtc 
gatatggac gaccataac cgcaccatg gtcttacaa 
gtcgataga accgattta aggctcata agcccagat 
cggaaagtt attttattg cacaagcaa cataaagat 
atcaccacg tgcatccaa ggagtgcag aaaatggaa 
catttcggg tttgtctgt cgcgaaggg aagaacgga 
caaactttc gtgggctat atcttcaaa tgcgagtcg 
tcttccatt gccacagac gcagtttca gccataacc 
caagccttc gccagttcg gaatcgcgc cctcgcccc 
gctatcaca tcctgtgac cattgcccg atggtctgg 
ttccataaa ctttgcaca gaaatcgaa cgaatgtcc 
gatcggaaa acccaagct gcgattttg cgccgtctt 
gaagaactc gacttggaa gaacagaac acaattttg 
gcaaaattc aaaggcgca gagacggac tcagtccgc 
gaacagaac gaattcctg atgatgtta ctcagagcc 
cattgcgaa atgaaacag ggccggcat gtgcacgac 
actgccgaa aatcgatca gaattttta aaccaatac 
ttgggtggc ggtggaacg atttttatg aaagcgaaa 
cgttccctt acaaattcg ttcgaaatg ttgaaacgt 
aaagcatcc aaggatgac tttggggcg agtttgcgt 
gaaatgaac agggaaaat tcgccacat tcgcccacg 
attgaggtc agtgataag gattcggaa gggatgagg 
tcaaggtca agtaccatt gggtcgcaa agcgattta 
cacttaatg gtacaagaa acaaaaagt tcagtttct 
ccagatcgc agtgttacc acgcctgac aggacacca 
aaatcgcca atgatggac atatttttg aaagttggt 
agtagccct aaatcggtc acttctgaa gatgggtcc 
ccgaagatc gactcgggg tcttggcgt caagccatt 
ttcaaaagg gtgattact cccagcaag aatgataaa 
cccgtggag atgaagcag aggactaag gaggagttg 
agggagttg tggaagaaa tcaatctat caggcgata 
cttctggtg aggatggag aaggaaaat gcacgactg 
aaggcagaa caggaggaa tcggccgtg aagaggatc 
aaattggag tatgacgag atgaagcct ccacagagg 
gaggtgatg gaggtctgg gagatgctg acgaataaa 
gaggcaagg atgaagtgt gataaccag atgctgttg 
caagctatt aggcaagga gtccctcgc ggaaaacgc 
ggcgaagtc tggcaattc ctcgccgag caatactgc 
atgcgtaca gcccccatc gacacctcc aacttcccc 
aactacaac gtcccctac gagcaactc ctcaaacaa 
ctaacctcg caccaacac gcaatttta atcgacttg 
ggccgtacc ttccccaac cattcgtac ttcagtagc 
cccttagga cctggccaa ctggccctc ttcaaccta 
ctcaaagcc tactcgtta ctcgactcc gaaatgggc 
tattgccaa ggcatgagt ttcgtcgct ggagtttta 
ctccttcac atggaagag tctcaagct ttcttcctg 
ttgcgacac ttgatgttt cgaagaggg ctcaggttg 
caatacttg ccagacatg gtggggtta caggtcaag 
ttgtaccaa ctgtccagg ttactccac gaccaactg 
cctgatctc tacaaccat ttcgatttc tacgaagtg 
gcaccaact ttgtatgcc gctccttgg ttattgact 
attttcgca tcgcagttt cctctaggt ttcgtcaca 
cgggttttt gacttaata tttttggag agccctgac 
gtcattttc cgagtggct atagctttg ttgaccttc 
cacaaagac aatctcctc gcttgcgac agttttgaa 
gaaataatg aactacttg aaaaacaaa ctaccaaca 
atagataaa cccacactt gacaaaatc atgaaacaa 
gtctacaca actgacata agcaagcaa ttgaatgag 
tacaaagtg gagtaccag gtcctacaa gaggaaatg 
acctccgtc cagccccaa gtcgaggct ttacacaag 
ctagaatct caaaacaag acactgact gaacagaac 
aaggccctg atggcccag ttagaactc gctttgagc 
aacgtccag cgactcgaa aaaacccgc actttacaa 
caggcccaa ctgaacaaa ctcgaaatg cacagtcgg 
gccttggac gtcaccatt gcaactttg ggcaacttc 
ctcaacact ttaatcgaa cagaaagtg gaaatagaa 
attccagac gacgttcga agaatcctc aaccaaatc 
tcttactcc gaaaagcgg aaaagcgag ctcaaaccc 
cagaacaac ttgatgaaa atgttccaa aagtccgaa 
aacgacaaa gccctagtt aagtccctc tcaactggc 
aaaataatg cttccggtc actctggac caaaacatt 
ttgcgttca aacagcctg gagaagtcc ccccagaaa 
acctcccag ttcttctcg tcttcacac aaccacatt 
ttgcaacaa ttcaacaac aacaacaaa atcgacatc 
aaggtgcag gagtgcgac ctgaacaag tccataagc 
ctgcctttg gactcctac cacgagaag gtctccccg 
tgcaactcc gtcgacagt ggcgtggga accccctcg 
agccccaaa ccaggcgac caccacccc ttgagcaac 
tgtgatgtg aacttcacc ttccatggg acgcgagaa 
ttgaagaat atcaagagt attaggaac atgtcccgg 
aactctagt ccggatatt atcggcaag tga
    mRNA sequence
    • >TC015747 RA
      ctcaaatgg ggcacaaaa tgggccaga gtctcccgg 
cctgaatac aggtgcagc gatggggtt gtccacgtt 
gtcgacgat gttttcgcc gcccccaag tttgcaaaa 
ccgccccaa acgcccaaa agtgggttt tggaggacg 
ttgaaagac gcgttcacg tgaccgccc tttgatttg 
ttgccaaac atttgcccg atttagtgc ccggtcata 
tttcgtggt aaaagtttg ttgattatt cagtgttgt 
tttatttaa aattttcct agaaataca gggtgattc 
aagtaagtt gtcacgctg aaaaatcga atgattaaa 
taatattgt tatgaaata tgagacttt acactttgt 
tatttttac tgtaactag tctatttat gtaagcaaa 
cgtcaaaac ctacaatta aaaaatggc cgtttgatt 
aacatgtat caatgtgtg gacacttat tgttaacac 
atagttcaa ctcttgtgt agaaaaagt tgatacgac 
agaggacgt cctatagtt gctactaac acgtgccat 
aaaaatctt agacaggtt tcggaactt gtcatttat 
aataaaacg ataaaataa aaatgttat tgcatccgc 
tgtaataat agaaacgaa gccccttat tagtgttat 
tgttgtggt aggattatt gcaatttgt ttccctacc 
ttggcgttt gacgtacac gatttccca gttaattaa 
aattttatt tgaaaaatt caactcgca attaatatt 
tttttgcaa cgagaaacc acttcatta cttaattgg 
aaataatat aagcttgat taaccaaaa atatttcaa 
ttttttttt ttcgaaatg catcgctct ctagaccgg 
tggccgcct cgttgcgac ctttccacc cgtgtatca 
cactttggg cgagtgatg cgttttaaa tttggccgt 
ccgtcgcga cgtcagcgt cgcttcctc gtgcgatag 
tttgatttt tcagtgcaa cagtgagtc gtgcgatgc 
ttcacgtct cgtgaaaaa tcatgataa cgataccaa 
actacggtt acaatccac ctgtgcgta tttcttcgc 
atagtgatg cctcctaca ttgaaaaga aaaccgttt 
cagaattgt ataaacaaa tgaaggacc aaaatcagg 
caccgacac gttctgtga gcagtgctg ccaatttaa 
catcactat gtgtggaca tatcgccca gttctagca 
atttcttcg aggttttat acattggga aaataaaag 
tttggcaaa agaaagtcc ctgatacgt tcattgatg 
atgctttgg agaaattca aaacacatg aaatggaaa 
aaatcaagg cgaaaatgt tgggcaata tcggacgat 
tgagaagtg aagataaca ttcgaagag gatcgaata 
gttcgacta gttcggttg aaagttacg ggcatcctc 
atttgcaaa gatcgcaat cttccatga gtggactta 
cagtgaaga ctgttgaag ataaagacg aagataagg 
aagatgatt caaagagtg tcgatatgg acgaccata 
accgcacca tggtcttac aagtcgata gaaccgatt 
taaggctca taagcccag atcggaaag ttattttat 
tgcacaagc aacataaag atatcacca cgtgcatcc 
aaggagtgc agaaaatgg aacatttcg ggtttgtct 
gtcgcgaag ggaagaacg gacaaactt tcgtgggct 
atatcttca aatgcgagt cgtcttcca ttgccacag 
acgcagttt cagccataa cccaagcct tcgccagtt 
cggaatcgc gccctcgcc ccgctatca catcctgtg 
accattgcc cgatggtct ggttccata aactttgca 
cagaaatcg aacgaatgt ccgatcgga aaacccaag 
ctgcgattt tgcgccgtc ttgaagaac tcgacttgg 
aagaacaga acacaattt tggcaaaat tcaaaggcg 
cagagacgg actcagtcc gcgaacaga acgaattcc 
tgatgatgt tactcagag cccattgcg aaatgaaac 
agggccggc atgtgcacg acactgccg aaaatcgat 
cagaatttt taaaccaat acttgggtg gcggtggaa 
cgattttta tgaaagcga aacgttccc ttacaaatt 
cgttcgaaa tgttgaaac gtaaagcat ccaaggatg 
actttgggg cgagtttgc gtgaaatga acagggaaa 
attcgccac attcgccca cgattgagg tcagtgata 
aggattcgg aagggatga ggtcaaggt caagtacca 
ttgggtcgc aaagcgatt tacacttaa tggtacaag 
aaacaaaaa gttcagttt ctccagatc gcagtgtta 
ccacgcctg acaggacac caaaatcgc caatgatgg 
acatatttt tgaaagttg gtagtagcc ctaaatcgg 
tcacttctg aagatgggt ccccgaaga tcgactcgg 
ggtcttggc gtcaagcca ttttcaaaa gggtgatta 
ctcccagca agaatgata aacccgtgg agatgaagc 
agaggacta aggaggagt tgagggagt tgtggaaga 
aatcaatct atcaggcga tacttctgg tgaggatgg 
agaaggaaa atgcacgac tgaaggcag aacaggagg 
aatcggccg tgaagagga tcaaattgg agtatgacg 
agatgaagc ctccacaga gggaggtga tggaggtct 
gggagatgc tgacgaata aagaggcaa ggatgaagt 
gtgataacc agatgctgt tgcaagcta ttaggcaag 
gagtccctc gcggaaaac gcggcgaag tctggcaat 
tcctcgccg agcaatact gcatgcgta cagccccca 
tcgacacct ccaacttcc ccaactaca acgtcccct 
acgagcaac tcctcaaac aactaacct cgcaccaac 
acgcaattt taatcgact tgggccgta ccttcccca 
accattcgt acttcagta gccccttag gacctggcc 
aactggccc tcttcaacc tactcaaag cctactcgt 
tactcgact ccgaaatgg gctattgcc aaggcatga 
gtttcgtcg ctggagttt tactccttc acatggaag 
agtctcaag ctttcttcc tgttgcgac acttgatgt 
ttcgaagag ggctcaggt tgcaatact tgccagaca 
tggtggggt tacaggtca agttgtacc aactgtcca 
ggttactcc acgaccaac tgcctgatc tctacaacc 
atttcgatt tctacgaag tggcaccaa ctttgtatg 
ccgctcctt ggttattga ctattttcg catcgcagt 
ttcctctag gtttcgtca cacgggttt ttgacttaa 
tatttttgg agagccctg acgtcattt tccgagtgg 
ctatagctt tgttgacct tccacaaag acaatctcc 
tcgcttgcg acagttttg aagaaataa tgaactact 
tgaaaaaca aactaccaa caatagata aacccacac 
ttgacaaaa tcatgaaac aagtctaca caactgaca 
taagcaagc aattgaatg agtacaaag tggagtacc 
aggtcctac aagaggaaa tgacctccg tccagcccc 
aagtcgagg ctttacaca agctagaat ctcaaaaca 
agacactga ctgaacaga acaaggccc tgatggccc 
agttagaac tcgctttga gcaacgtcc agcgactcg 
aaaaaaccc gcactttac aacaggccc aactgaaca 
aactcgaaa tgcacagtc gggccttgg acgtcacca 
ttgcaactt tgggcaact tcctcaaca ctttaatcg 
aacagaaag tggaaatag aaattccag acgacgttc 
gaagaatcc tcaaccaaa tctcttact ccgaaaagc 
ggaaaagcg agctcaaac cccagaaca acttgatga 
aaatgttcc aaaagtccg aaaacgaca aagccctag 
ttaagtccc tctcaactg gcaaaataa tgcttccgg 
tcactctgg accaaaaca ttttgcgtt caaacagcc 
tggagaagt ccccccaga aaacctccc agttcttct 
cgtcttcac acaaccaca ttttgcaac aattcaaca 
acaacaaca aaatcgaca tcaaggtgc aggagtgcg 
acctgaaca agtccataa gcctgcctt tggactcct 
accacgaga aggtctccc cgtgcaact ccgtcgaca 
gtggcgtgg gaaccccct cgagcccca aaccaggcg 
accaccacc ccttgagca actgtgatg tgaacttca 
ccttccatg ggacgcgag aattgaaga atatcaaga 
gtattagga acatgtccc ggaactcta gtccggata 
ttatcggca agtgatatt agggcgcgt gtacttatt 
ttactggta ttatgttgc tattgtgat ttgtgaata 
gtatttaag aataaagta gttgtttat at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY