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Gene information
General information
TC015747 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7365529..7391113
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015747
    iBeetle screen iB_02824

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015747 RA
      MITIPNYGY NPPVRISSH SDASYIEKK TVSELYKQM 
      KDQNQAPTR SVSSAANLT SLCVDISPS SSNFFEVLY 
      IGKIKVWQK KVPDTFIDD ALEKFKTHE MEKIKAKML 
      GNIGRLRSE DNIRRGSNS STSSVESYG HPHLQRSQS 
      SMSGLTVKT VEDKDEDKE DDSKSVDMD DHNRTMVLQ 
      VDRTDLRLI SPDRKVILL HKQHKDITT CIQGVQKME 
      HFGFVCREG KNGQTFVGY IFKCESSSI ATDAVSAIT 
      QAFASSESR PRPAITSCD HCPMVWFHK LCTEIERMS 
      DRKTQAAIL RRLEELDLE EQNTILAKF KGAETDSVR 
      EQNEFLMML LRAHCEMKQ GRHVHDTAE NRSEFLNQY 
      LGGGGTIFM KAKRSLTNS FEMLKRKAS KDDFGASLR 
      EMNRENSPH SPTIEVSDK DSEGMRSRS STIGSQSDL 
      HLMVQETKS SVSPDRSVT TPDRTPKSP MMDIFLKVG 
      SSPKSVTSE DGSPKIDSG SWRQAIFKR VITPSKNDK 
      PVEMKQRTK EELRELWKK SIYQAILLV RMEKENARL 
      KAEQEESAV KRIKLEYDE MKPPQREVM EVWEMLTNK 
      EARMKCDNQ MLLQAIRQG VPRGKRGEV WQFLAEQYC 
      MRTAPIDTS NFPNYNVPY EQLLKQLTS HQHAILIDL 
      GRTFPNHSY FSSPLGPGQ LALFNLLKA YSLLDSEMG 
      YCQGMSFVA GVLLLHMEE SQAFFLLRH LMFRRGLRL 
      QYLPDMVGL QVKLYQLSR LLHDQLPDL YNHFDFYEV 
      APTLYAAPW LLTIFASQF PLGFVTRVF DLIFLESPD 
      VIFRVAIAL LTFHKDNLL ACDSFEEIM NYLKNKLPT 
      IDKPTLDKI MKQVYTTDI SKQLNEYKV EYQVLQEEM 
      TSVQPQVEA LHKLESQNK TLTEQNKAL MAQLELALS 
      NVQRLEKTR TLQQAQLNK LEMHSRALD VTIATLGNF 
      LNTLIEQKV EIEIPDDVR RILNQISYS EKRKSELKP 
      QNNLMKMFQ KSENDKALV KSLSTGKIM LPVTLDQNI 
      LRSNSLEKS PQKTSQFFS SSHNHILQQ FNNNNKIDI 
      KVQECDLNK SISLPLDSY HEKVSPCNS VDSGVGTPS 
      SPKPGDHHP LSNCDVNFT FHGTRELKN IKSIRNMSR 
      NSSPDIIGK
    CDS sequence
    • >TC015747 RA
      atgataacg ataccaaac tacggttac aatccacct 
      gtgcgtatt tcttcgcat agtgatgcc tcctacatt 
      gaaaagaaa accgtttca gaattgtat aaacaaatg 
      aaggaccaa aatcaggca ccgacacgt tctgtgagc 
      agtgctgcc aatttaaca tcactatgt gtggacata 
      tcgcccagt tctagcaat ttcttcgag gttttatac 
      attgggaaa ataaaagtt tggcaaaag aaagtccct 
      gatacgttc attgatgat gctttggag aaattcaaa 
      acacatgaa atggaaaaa atcaaggcg aaaatgttg 
      ggcaatatc ggacgattg agaagtgaa gataacatt 
      cgaagagga tcgaatagt tcgactagt tcggttgaa 
      agttacggg catcctcat ttgcaaaga tcgcaatct 
      tccatgagt ggacttaca gtgaagact gttgaagat 
      aaagacgaa gataaggaa gatgattca aagagtgtc 
      gatatggac gaccataac cgcaccatg gtcttacaa 
      gtcgataga accgattta aggctcata agcccagat 
      cggaaagtt attttattg cacaagcaa cataaagat 
      atcaccacg tgcatccaa ggagtgcag aaaatggaa 
      catttcggg tttgtctgt cgcgaaggg aagaacgga 
      caaactttc gtgggctat atcttcaaa tgcgagtcg 
      tcttccatt gccacagac gcagtttca gccataacc 
      caagccttc gccagttcg gaatcgcgc cctcgcccc 
      gctatcaca tcctgtgac cattgcccg atggtctgg 
      ttccataaa ctttgcaca gaaatcgaa cgaatgtcc 
      gatcggaaa acccaagct gcgattttg cgccgtctt 
      gaagaactc gacttggaa gaacagaac acaattttg 
      gcaaaattc aaaggcgca gagacggac tcagtccgc 
      gaacagaac gaattcctg atgatgtta ctcagagcc 
      cattgcgaa atgaaacag ggccggcat gtgcacgac 
      actgccgaa aatcgatca gaattttta aaccaatac 
      ttgggtggc ggtggaacg atttttatg aaagcgaaa 
      cgttccctt acaaattcg ttcgaaatg ttgaaacgt 
      aaagcatcc aaggatgac tttggggcg agtttgcgt 
      gaaatgaac agggaaaat tcgccacat tcgcccacg 
      attgaggtc agtgataag gattcggaa gggatgagg 
      tcaaggtca agtaccatt gggtcgcaa agcgattta 
      cacttaatg gtacaagaa acaaaaagt tcagtttct 
      ccagatcgc agtgttacc acgcctgac aggacacca 
      aaatcgcca atgatggac atatttttg aaagttggt 
      agtagccct aaatcggtc acttctgaa gatgggtcc 
      ccgaagatc gactcgggg tcttggcgt caagccatt 
      ttcaaaagg gtgattact cccagcaag aatgataaa 
      cccgtggag atgaagcag aggactaag gaggagttg 
      agggagttg tggaagaaa tcaatctat caggcgata 
      cttctggtg aggatggag aaggaaaat gcacgactg 
      aaggcagaa caggaggaa tcggccgtg aagaggatc 
      aaattggag tatgacgag atgaagcct ccacagagg 
      gaggtgatg gaggtctgg gagatgctg acgaataaa 
      gaggcaagg atgaagtgt gataaccag atgctgttg 
      caagctatt aggcaagga gtccctcgc ggaaaacgc 
      ggcgaagtc tggcaattc ctcgccgag caatactgc 
      atgcgtaca gcccccatc gacacctcc aacttcccc 
      aactacaac gtcccctac gagcaactc ctcaaacaa 
      ctaacctcg caccaacac gcaatttta atcgacttg 
      ggccgtacc ttccccaac cattcgtac ttcagtagc 
      cccttagga cctggccaa ctggccctc ttcaaccta 
      ctcaaagcc tactcgtta ctcgactcc gaaatgggc 
      tattgccaa ggcatgagt ttcgtcgct ggagtttta 
      ctccttcac atggaagag tctcaagct ttcttcctg 
      ttgcgacac ttgatgttt cgaagaggg ctcaggttg 
      caatacttg ccagacatg gtggggtta caggtcaag 
      ttgtaccaa ctgtccagg ttactccac gaccaactg 
      cctgatctc tacaaccat ttcgatttc tacgaagtg 
      gcaccaact ttgtatgcc gctccttgg ttattgact 
      attttcgca tcgcagttt cctctaggt ttcgtcaca 
      cgggttttt gacttaata tttttggag agccctgac 
      gtcattttc cgagtggct atagctttg ttgaccttc 
      cacaaagac aatctcctc gcttgcgac agttttgaa 
      gaaataatg aactacttg aaaaacaaa ctaccaaca 
      atagataaa cccacactt gacaaaatc atgaaacaa 
      gtctacaca actgacata agcaagcaa ttgaatgag 
      tacaaagtg gagtaccag gtcctacaa gaggaaatg 
      acctccgtc cagccccaa gtcgaggct ttacacaag 
      ctagaatct caaaacaag acactgact gaacagaac 
      aaggccctg atggcccag ttagaactc gctttgagc 
      aacgtccag cgactcgaa aaaacccgc actttacaa 
      caggcccaa ctgaacaaa ctcgaaatg cacagtcgg 
      gccttggac gtcaccatt gcaactttg ggcaacttc 
      ctcaacact ttaatcgaa cagaaagtg gaaatagaa 
      attccagac gacgttcga agaatcctc aaccaaatc 
      tcttactcc gaaaagcgg aaaagcgag ctcaaaccc 
      cagaacaac ttgatgaaa atgttccaa aagtccgaa 
      aacgacaaa gccctagtt aagtccctc tcaactggc 
      aaaataatg cttccggtc actctggac caaaacatt 
      ttgcgttca aacagcctg gagaagtcc ccccagaaa 
      acctcccag ttcttctcg tcttcacac aaccacatt 
      ttgcaacaa ttcaacaac aacaacaaa atcgacatc 
      aaggtgcag gagtgcgac ctgaacaag tccataagc 
      ctgcctttg gactcctac cacgagaag gtctccccg 
      tgcaactcc gtcgacagt ggcgtggga accccctcg 
      agccccaaa ccaggcgac caccacccc ttgagcaac 
      tgtgatgtg aacttcacc ttccatggg acgcgagaa 
      ttgaagaat atcaagagt attaggaac atgtcccgg 
      aactctagt ccggatatt atcggcaag tga
    mRNA sequence
    • >TC015747 RA
      ctcaaatgg ggcacaaaa tgggccaga gtctcccgg 
      cctgaatac aggtgcagc gatggggtt gtccacgtt 
      gtcgacgat gttttcgcc gcccccaag tttgcaaaa 
      ccgccccaa acgcccaaa agtgggttt tggaggacg 
      ttgaaagac gcgttcacg tgaccgccc tttgatttg 
      ttgccaaac atttgcccg atttagtgc ccggtcata 
      tttcgtggt aaaagtttg ttgattatt cagtgttgt 
      tttatttaa aattttcct agaaataca gggtgattc 
      aagtaagtt gtcacgctg aaaaatcga atgattaaa 
      taatattgt tatgaaata tgagacttt acactttgt 
      tatttttac tgtaactag tctatttat gtaagcaaa 
      cgtcaaaac ctacaatta aaaaatggc cgtttgatt 
      aacatgtat caatgtgtg gacacttat tgttaacac 
      atagttcaa ctcttgtgt agaaaaagt tgatacgac 
      agaggacgt cctatagtt gctactaac acgtgccat 
      aaaaatctt agacaggtt tcggaactt gtcatttat 
      aataaaacg ataaaataa aaatgttat tgcatccgc 
      tgtaataat agaaacgaa gccccttat tagtgttat 
      tgttgtggt aggattatt gcaatttgt ttccctacc 
      ttggcgttt gacgtacac gatttccca gttaattaa 
      aattttatt tgaaaaatt caactcgca attaatatt 
      tttttgcaa cgagaaacc acttcatta cttaattgg 
      aaataatat aagcttgat taaccaaaa atatttcaa 
      ttttttttt ttcgaaatg catcgctct ctagaccgg 
      tggccgcct cgttgcgac ctttccacc cgtgtatca 
      cactttggg cgagtgatg cgttttaaa tttggccgt 
      ccgtcgcga cgtcagcgt cgcttcctc gtgcgatag 
      tttgatttt tcagtgcaa cagtgagtc gtgcgatgc 
      ttcacgtct cgtgaaaaa tcatgataa cgataccaa 
      actacggtt acaatccac ctgtgcgta tttcttcgc 
      atagtgatg cctcctaca ttgaaaaga aaaccgttt 
      cagaattgt ataaacaaa tgaaggacc aaaatcagg 
      caccgacac gttctgtga gcagtgctg ccaatttaa 
      catcactat gtgtggaca tatcgccca gttctagca 
      atttcttcg aggttttat acattggga aaataaaag 
      tttggcaaa agaaagtcc ctgatacgt tcattgatg 
      atgctttgg agaaattca aaacacatg aaatggaaa 
      aaatcaagg cgaaaatgt tgggcaata tcggacgat 
      tgagaagtg aagataaca ttcgaagag gatcgaata 
      gttcgacta gttcggttg aaagttacg ggcatcctc 
      atttgcaaa gatcgcaat cttccatga gtggactta 
      cagtgaaga ctgttgaag ataaagacg aagataagg 
      aagatgatt caaagagtg tcgatatgg acgaccata 
      accgcacca tggtcttac aagtcgata gaaccgatt 
      taaggctca taagcccag atcggaaag ttattttat 
      tgcacaagc aacataaag atatcacca cgtgcatcc 
      aaggagtgc agaaaatgg aacatttcg ggtttgtct 
      gtcgcgaag ggaagaacg gacaaactt tcgtgggct 
      atatcttca aatgcgagt cgtcttcca ttgccacag 
      acgcagttt cagccataa cccaagcct tcgccagtt 
      cggaatcgc gccctcgcc ccgctatca catcctgtg 
      accattgcc cgatggtct ggttccata aactttgca 
      cagaaatcg aacgaatgt ccgatcgga aaacccaag 
      ctgcgattt tgcgccgtc ttgaagaac tcgacttgg 
      aagaacaga acacaattt tggcaaaat tcaaaggcg 
      cagagacgg actcagtcc gcgaacaga acgaattcc 
      tgatgatgt tactcagag cccattgcg aaatgaaac 
      agggccggc atgtgcacg acactgccg aaaatcgat 
      cagaatttt taaaccaat acttgggtg gcggtggaa 
      cgattttta tgaaagcga aacgttccc ttacaaatt 
      cgttcgaaa tgttgaaac gtaaagcat ccaaggatg 
      actttgggg cgagtttgc gtgaaatga acagggaaa 
      attcgccac attcgccca cgattgagg tcagtgata 
      aggattcgg aagggatga ggtcaaggt caagtacca 
      ttgggtcgc aaagcgatt tacacttaa tggtacaag 
      aaacaaaaa gttcagttt ctccagatc gcagtgtta 
      ccacgcctg acaggacac caaaatcgc caatgatgg 
      acatatttt tgaaagttg gtagtagcc ctaaatcgg 
      tcacttctg aagatgggt ccccgaaga tcgactcgg 
      ggtcttggc gtcaagcca ttttcaaaa gggtgatta 
      ctcccagca agaatgata aacccgtgg agatgaagc 
      agaggacta aggaggagt tgagggagt tgtggaaga 
      aatcaatct atcaggcga tacttctgg tgaggatgg 
      agaaggaaa atgcacgac tgaaggcag aacaggagg 
      aatcggccg tgaagagga tcaaattgg agtatgacg 
      agatgaagc ctccacaga gggaggtga tggaggtct 
      gggagatgc tgacgaata aagaggcaa ggatgaagt 
      gtgataacc agatgctgt tgcaagcta ttaggcaag 
      gagtccctc gcggaaaac gcggcgaag tctggcaat 
      tcctcgccg agcaatact gcatgcgta cagccccca 
      tcgacacct ccaacttcc ccaactaca acgtcccct 
      acgagcaac tcctcaaac aactaacct cgcaccaac 
      acgcaattt taatcgact tgggccgta ccttcccca 
      accattcgt acttcagta gccccttag gacctggcc 
      aactggccc tcttcaacc tactcaaag cctactcgt 
      tactcgact ccgaaatgg gctattgcc aaggcatga 
      gtttcgtcg ctggagttt tactccttc acatggaag 
      agtctcaag ctttcttcc tgttgcgac acttgatgt 
      ttcgaagag ggctcaggt tgcaatact tgccagaca 
      tggtggggt tacaggtca agttgtacc aactgtcca 
      ggttactcc acgaccaac tgcctgatc tctacaacc 
      atttcgatt tctacgaag tggcaccaa ctttgtatg 
      ccgctcctt ggttattga ctattttcg catcgcagt 
      ttcctctag gtttcgtca cacgggttt ttgacttaa 
      tatttttgg agagccctg acgtcattt tccgagtgg 
      ctatagctt tgttgacct tccacaaag acaatctcc 
      tcgcttgcg acagttttg aagaaataa tgaactact 
      tgaaaaaca aactaccaa caatagata aacccacac 
      ttgacaaaa tcatgaaac aagtctaca caactgaca 
      taagcaagc aattgaatg agtacaaag tggagtacc 
      aggtcctac aagaggaaa tgacctccg tccagcccc 
      aagtcgagg ctttacaca agctagaat ctcaaaaca 
      agacactga ctgaacaga acaaggccc tgatggccc 
      agttagaac tcgctttga gcaacgtcc agcgactcg 
      aaaaaaccc gcactttac aacaggccc aactgaaca 
      aactcgaaa tgcacagtc gggccttgg acgtcacca 
      ttgcaactt tgggcaact tcctcaaca ctttaatcg 
      aacagaaag tggaaatag aaattccag acgacgttc 
      gaagaatcc tcaaccaaa tctcttact ccgaaaagc 
      ggaaaagcg agctcaaac cccagaaca acttgatga 
      aaatgttcc aaaagtccg aaaacgaca aagccctag 
      ttaagtccc tctcaactg gcaaaataa tgcttccgg 
      tcactctgg accaaaaca ttttgcgtt caaacagcc 
      tggagaagt ccccccaga aaacctccc agttcttct 
      cgtcttcac acaaccaca ttttgcaac aattcaaca 
      acaacaaca aaatcgaca tcaaggtgc aggagtgcg 
      acctgaaca agtccataa gcctgcctt tggactcct 
      accacgaga aggtctccc cgtgcaact ccgtcgaca 
      gtggcgtgg gaaccccct cgagcccca aaccaggcg 
      accaccacc ccttgagca actgtgatg tgaacttca 
      ccttccatg ggacgcgag aattgaaga atatcaaga 
      gtattagga acatgtccc ggaactcta gtccggata 
      ttatcggca agtgatatt agggcgcgt gtacttatt 
      ttactggta ttatgttgc tattgtgat ttgtgaata 
      gtatttaag aataaagta gttgtttat at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY