General information | |||
TC015747 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 7365529..7391113 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015747 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02824 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC015747 RA
MITIPNYGY NPPVRISSH SDASYIEKK TVSELYKQM KDQNQAPTR SVSSAANLT SLCVDISPS SSNFFEVLY IGKIKVWQK KVPDTFIDD ALEKFKTHE MEKIKAKML GNIGRLRSE DNIRRGSNS STSSVESYG HPHLQRSQS SMSGLTVKT VEDKDEDKE DDSKSVDMD DHNRTMVLQ VDRTDLRLI SPDRKVILL HKQHKDITT CIQGVQKME HFGFVCREG KNGQTFVGY IFKCESSSI ATDAVSAIT QAFASSESR PRPAITSCD HCPMVWFHK LCTEIERMS DRKTQAAIL RRLEELDLE EQNTILAKF KGAETDSVR EQNEFLMML LRAHCEMKQ GRHVHDTAE NRSEFLNQY LGGGGTIFM KAKRSLTNS FEMLKRKAS KDDFGASLR EMNRENSPH SPTIEVSDK DSEGMRSRS STIGSQSDL HLMVQETKS SVSPDRSVT TPDRTPKSP MMDIFLKVG SSPKSVTSE DGSPKIDSG SWRQAIFKR VITPSKNDK PVEMKQRTK EELRELWKK SIYQAILLV RMEKENARL KAEQEESAV KRIKLEYDE MKPPQREVM EVWEMLTNK EARMKCDNQ MLLQAIRQG VPRGKRGEV WQFLAEQYC MRTAPIDTS NFPNYNVPY EQLLKQLTS HQHAILIDL GRTFPNHSY FSSPLGPGQ LALFNLLKA YSLLDSEMG YCQGMSFVA GVLLLHMEE SQAFFLLRH LMFRRGLRL QYLPDMVGL QVKLYQLSR LLHDQLPDL YNHFDFYEV APTLYAAPW LLTIFASQF PLGFVTRVF DLIFLESPD VIFRVAIAL LTFHKDNLL ACDSFEEIM NYLKNKLPT IDKPTLDKI MKQVYTTDI SKQLNEYKV EYQVLQEEM TSVQPQVEA LHKLESQNK TLTEQNKAL MAQLELALS NVQRLEKTR TLQQAQLNK LEMHSRALD VTIATLGNF LNTLIEQKV EIEIPDDVR RILNQISYS EKRKSELKP QNNLMKMFQ KSENDKALV KSLSTGKIM LPVTLDQNI LRSNSLEKS PQKTSQFFS SSHNHILQQ FNNNNKIDI KVQECDLNK SISLPLDSY HEKVSPCNS VDSGVGTPS SPKPGDHHP LSNCDVNFT FHGTRELKN IKSIRNMSR NSSPDIIGK
- >TC015747 RA
atgataacg ataccaaac tacggttac aatccacct gtgcgtatt tcttcgcat agtgatgcc tcctacatt gaaaagaaa accgtttca gaattgtat aaacaaatg aaggaccaa aatcaggca ccgacacgt tctgtgagc agtgctgcc aatttaaca tcactatgt gtggacata tcgcccagt tctagcaat ttcttcgag gttttatac attgggaaa ataaaagtt tggcaaaag aaagtccct gatacgttc attgatgat gctttggag aaattcaaa acacatgaa atggaaaaa atcaaggcg aaaatgttg ggcaatatc ggacgattg agaagtgaa gataacatt cgaagagga tcgaatagt tcgactagt tcggttgaa agttacggg catcctcat ttgcaaaga tcgcaatct tccatgagt ggacttaca gtgaagact gttgaagat aaagacgaa gataaggaa gatgattca aagagtgtc gatatggac gaccataac cgcaccatg gtcttacaa gtcgataga accgattta aggctcata agcccagat cggaaagtt attttattg cacaagcaa cataaagat atcaccacg tgcatccaa ggagtgcag aaaatggaa catttcggg tttgtctgt cgcgaaggg aagaacgga caaactttc gtgggctat atcttcaaa tgcgagtcg tcttccatt gccacagac gcagtttca gccataacc caagccttc gccagttcg gaatcgcgc cctcgcccc gctatcaca tcctgtgac cattgcccg atggtctgg ttccataaa ctttgcaca gaaatcgaa cgaatgtcc gatcggaaa acccaagct gcgattttg cgccgtctt gaagaactc gacttggaa gaacagaac acaattttg gcaaaattc aaaggcgca gagacggac tcagtccgc gaacagaac gaattcctg atgatgtta ctcagagcc cattgcgaa atgaaacag ggccggcat gtgcacgac actgccgaa aatcgatca gaattttta aaccaatac ttgggtggc ggtggaacg atttttatg aaagcgaaa cgttccctt acaaattcg ttcgaaatg ttgaaacgt aaagcatcc aaggatgac tttggggcg agtttgcgt gaaatgaac agggaaaat tcgccacat tcgcccacg attgaggtc agtgataag gattcggaa gggatgagg tcaaggtca agtaccatt gggtcgcaa agcgattta cacttaatg gtacaagaa acaaaaagt tcagtttct ccagatcgc agtgttacc acgcctgac aggacacca aaatcgcca atgatggac atatttttg aaagttggt agtagccct aaatcggtc acttctgaa gatgggtcc ccgaagatc gactcgggg tcttggcgt caagccatt ttcaaaagg gtgattact cccagcaag aatgataaa cccgtggag atgaagcag aggactaag gaggagttg agggagttg tggaagaaa tcaatctat caggcgata cttctggtg aggatggag aaggaaaat gcacgactg aaggcagaa caggaggaa tcggccgtg aagaggatc aaattggag tatgacgag atgaagcct ccacagagg gaggtgatg gaggtctgg gagatgctg acgaataaa gaggcaagg atgaagtgt gataaccag atgctgttg caagctatt aggcaagga gtccctcgc ggaaaacgc ggcgaagtc tggcaattc ctcgccgag caatactgc atgcgtaca gcccccatc gacacctcc aacttcccc aactacaac gtcccctac gagcaactc ctcaaacaa ctaacctcg caccaacac gcaatttta atcgacttg ggccgtacc ttccccaac cattcgtac ttcagtagc cccttagga cctggccaa ctggccctc ttcaaccta ctcaaagcc tactcgtta ctcgactcc gaaatgggc tattgccaa ggcatgagt ttcgtcgct ggagtttta ctccttcac atggaagag tctcaagct ttcttcctg ttgcgacac ttgatgttt cgaagaggg ctcaggttg caatacttg ccagacatg gtggggtta caggtcaag ttgtaccaa ctgtccagg ttactccac gaccaactg cctgatctc tacaaccat ttcgatttc tacgaagtg gcaccaact ttgtatgcc gctccttgg ttattgact attttcgca tcgcagttt cctctaggt ttcgtcaca cgggttttt gacttaata tttttggag agccctgac gtcattttc cgagtggct atagctttg ttgaccttc cacaaagac aatctcctc gcttgcgac agttttgaa gaaataatg aactacttg aaaaacaaa ctaccaaca atagataaa cccacactt gacaaaatc atgaaacaa gtctacaca actgacata agcaagcaa ttgaatgag tacaaagtg gagtaccag gtcctacaa gaggaaatg acctccgtc cagccccaa gtcgaggct ttacacaag ctagaatct caaaacaag acactgact gaacagaac aaggccctg atggcccag ttagaactc gctttgagc aacgtccag cgactcgaa aaaacccgc actttacaa caggcccaa ctgaacaaa ctcgaaatg cacagtcgg gccttggac gtcaccatt gcaactttg ggcaacttc ctcaacact ttaatcgaa cagaaagtg gaaatagaa attccagac gacgttcga agaatcctc aaccaaatc tcttactcc gaaaagcgg aaaagcgag ctcaaaccc cagaacaac ttgatgaaa atgttccaa aagtccgaa aacgacaaa gccctagtt aagtccctc tcaactggc aaaataatg cttccggtc actctggac caaaacatt ttgcgttca aacagcctg gagaagtcc ccccagaaa acctcccag ttcttctcg tcttcacac aaccacatt ttgcaacaa ttcaacaac aacaacaaa atcgacatc aaggtgcag gagtgcgac ctgaacaag tccataagc ctgcctttg gactcctac cacgagaag gtctccccg tgcaactcc gtcgacagt ggcgtggga accccctcg agccccaaa ccaggcgac caccacccc ttgagcaac tgtgatgtg aacttcacc ttccatggg acgcgagaa ttgaagaat atcaagagt attaggaac atgtcccgg aactctagt ccggatatt atcggcaag tga
- >TC015747 RA
ctcaaatgg ggcacaaaa tgggccaga gtctcccgg cctgaatac aggtgcagc gatggggtt gtccacgtt gtcgacgat gttttcgcc gcccccaag tttgcaaaa ccgccccaa acgcccaaa agtgggttt tggaggacg ttgaaagac gcgttcacg tgaccgccc tttgatttg ttgccaaac atttgcccg atttagtgc ccggtcata tttcgtggt aaaagtttg ttgattatt cagtgttgt tttatttaa aattttcct agaaataca gggtgattc aagtaagtt gtcacgctg aaaaatcga atgattaaa taatattgt tatgaaata tgagacttt acactttgt tatttttac tgtaactag tctatttat gtaagcaaa cgtcaaaac ctacaatta aaaaatggc cgtttgatt aacatgtat caatgtgtg gacacttat tgttaacac atagttcaa ctcttgtgt agaaaaagt tgatacgac agaggacgt cctatagtt gctactaac acgtgccat aaaaatctt agacaggtt tcggaactt gtcatttat aataaaacg ataaaataa aaatgttat tgcatccgc tgtaataat agaaacgaa gccccttat tagtgttat tgttgtggt aggattatt gcaatttgt ttccctacc ttggcgttt gacgtacac gatttccca gttaattaa aattttatt tgaaaaatt caactcgca attaatatt tttttgcaa cgagaaacc acttcatta cttaattgg aaataatat aagcttgat taaccaaaa atatttcaa ttttttttt ttcgaaatg catcgctct ctagaccgg tggccgcct cgttgcgac ctttccacc cgtgtatca cactttggg cgagtgatg cgttttaaa tttggccgt ccgtcgcga cgtcagcgt cgcttcctc gtgcgatag tttgatttt tcagtgcaa cagtgagtc gtgcgatgc ttcacgtct cgtgaaaaa tcatgataa cgataccaa actacggtt acaatccac ctgtgcgta tttcttcgc atagtgatg cctcctaca ttgaaaaga aaaccgttt cagaattgt ataaacaaa tgaaggacc aaaatcagg caccgacac gttctgtga gcagtgctg ccaatttaa catcactat gtgtggaca tatcgccca gttctagca atttcttcg aggttttat acattggga aaataaaag tttggcaaa agaaagtcc ctgatacgt tcattgatg atgctttgg agaaattca aaacacatg aaatggaaa aaatcaagg cgaaaatgt tgggcaata tcggacgat tgagaagtg aagataaca ttcgaagag gatcgaata gttcgacta gttcggttg aaagttacg ggcatcctc atttgcaaa gatcgcaat cttccatga gtggactta cagtgaaga ctgttgaag ataaagacg aagataagg aagatgatt caaagagtg tcgatatgg acgaccata accgcacca tggtcttac aagtcgata gaaccgatt taaggctca taagcccag atcggaaag ttattttat tgcacaagc aacataaag atatcacca cgtgcatcc aaggagtgc agaaaatgg aacatttcg ggtttgtct gtcgcgaag ggaagaacg gacaaactt tcgtgggct atatcttca aatgcgagt cgtcttcca ttgccacag acgcagttt cagccataa cccaagcct tcgccagtt cggaatcgc gccctcgcc ccgctatca catcctgtg accattgcc cgatggtct ggttccata aactttgca cagaaatcg aacgaatgt ccgatcgga aaacccaag ctgcgattt tgcgccgtc ttgaagaac tcgacttgg aagaacaga acacaattt tggcaaaat tcaaaggcg cagagacgg actcagtcc gcgaacaga acgaattcc tgatgatgt tactcagag cccattgcg aaatgaaac agggccggc atgtgcacg acactgccg aaaatcgat cagaatttt taaaccaat acttgggtg gcggtggaa cgattttta tgaaagcga aacgttccc ttacaaatt cgttcgaaa tgttgaaac gtaaagcat ccaaggatg actttgggg cgagtttgc gtgaaatga acagggaaa attcgccac attcgccca cgattgagg tcagtgata aggattcgg aagggatga ggtcaaggt caagtacca ttgggtcgc aaagcgatt tacacttaa tggtacaag aaacaaaaa gttcagttt ctccagatc gcagtgtta ccacgcctg acaggacac caaaatcgc caatgatgg acatatttt tgaaagttg gtagtagcc ctaaatcgg tcacttctg aagatgggt ccccgaaga tcgactcgg ggtcttggc gtcaagcca ttttcaaaa gggtgatta ctcccagca agaatgata aacccgtgg agatgaagc agaggacta aggaggagt tgagggagt tgtggaaga aatcaatct atcaggcga tacttctgg tgaggatgg agaaggaaa atgcacgac tgaaggcag aacaggagg aatcggccg tgaagagga tcaaattgg agtatgacg agatgaagc ctccacaga gggaggtga tggaggtct gggagatgc tgacgaata aagaggcaa ggatgaagt gtgataacc agatgctgt tgcaagcta ttaggcaag gagtccctc gcggaaaac gcggcgaag tctggcaat tcctcgccg agcaatact gcatgcgta cagccccca tcgacacct ccaacttcc ccaactaca acgtcccct acgagcaac tcctcaaac aactaacct cgcaccaac acgcaattt taatcgact tgggccgta ccttcccca accattcgt acttcagta gccccttag gacctggcc aactggccc tcttcaacc tactcaaag cctactcgt tactcgact ccgaaatgg gctattgcc aaggcatga gtttcgtcg ctggagttt tactccttc acatggaag agtctcaag ctttcttcc tgttgcgac acttgatgt ttcgaagag ggctcaggt tgcaatact tgccagaca tggtggggt tacaggtca agttgtacc aactgtcca ggttactcc acgaccaac tgcctgatc tctacaacc atttcgatt tctacgaag tggcaccaa ctttgtatg ccgctcctt ggttattga ctattttcg catcgcagt ttcctctag gtttcgtca cacgggttt ttgacttaa tatttttgg agagccctg acgtcattt tccgagtgg ctatagctt tgttgacct tccacaaag acaatctcc tcgcttgcg acagttttg aagaaataa tgaactact tgaaaaaca aactaccaa caatagata aacccacac ttgacaaaa tcatgaaac aagtctaca caactgaca taagcaagc aattgaatg agtacaaag tggagtacc aggtcctac aagaggaaa tgacctccg tccagcccc aagtcgagg ctttacaca agctagaat ctcaaaaca agacactga ctgaacaga acaaggccc tgatggccc agttagaac tcgctttga gcaacgtcc agcgactcg aaaaaaccc gcactttac aacaggccc aactgaaca aactcgaaa tgcacagtc gggccttgg acgtcacca ttgcaactt tgggcaact tcctcaaca ctttaatcg aacagaaag tggaaatag aaattccag acgacgttc gaagaatcc tcaaccaaa tctcttact ccgaaaagc ggaaaagcg agctcaaac cccagaaca acttgatga aaatgttcc aaaagtccg aaaacgaca aagccctag ttaagtccc tctcaactg gcaaaataa tgcttccgg tcactctgg accaaaaca ttttgcgtt caaacagcc tggagaagt ccccccaga aaacctccc agttcttct cgtcttcac acaaccaca ttttgcaac aattcaaca acaacaaca aaatcgaca tcaaggtgc aggagtgcg acctgaaca agtccataa gcctgcctt tggactcct accacgaga aggtctccc cgtgcaact ccgtcgaca gtggcgtgg gaaccccct cgagcccca aaccaggcg accaccacc ccttgagca actgtgatg tgaacttca ccttccatg ggacgcgag aattgaaga atatcaaga gtattagga acatgtccc ggaactcta gtccggata ttatcggca agtgatatt agggcgcgt gtacttatt ttactggta ttatgttgc tattgtgat ttgtgaata gtatttaag aataaagta gttgtttat at
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
