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Gene information
General information
TC015680 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5060023..5070356
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015680
    iBeetle screen iB_02822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015680 RA
      MGLYQLEEQ PPIVKAIFA GCEPEKYKE LIKSKQDVN 
      CLDSKKRTP LHAAAFTGD AAIASVLLS NGARVNAKD 
      TKWLTPLHQ ACYIRSSET VSILLNNNA DVNARDKLW 
      QTPLHVAAA NGAYKCIEQ LLNHVPNPN VTDRGGRTA 
      LHLAAYSEM ADCVELLIS GGCIVNAYD KKDCRPLHR 
      AVQVGSLSI IELLLKYKA EINAKDRNQ YTPLHVAAA 
      GGTDAVCRL LISHGADVN AQNVFGNTP LHIACLNGH 
      HLVCQELIN SGADIEAVN YRGQTPLHI AAVSTNGVD 
      CMMLLLTQK VDINRQSLD GRTPLHMTA IHGRFTRSK 
      ILIDKGATI DCPDKNDCT PLHIAARYG HDLLTNTLL 
      SYGANPSQR GYEGRQPLH MCCLAGYVE CCRKLLQAG 
      VDLNAVDDS GKTPTHCAA YKGSVECLD LLVSNGAKF 
      QLKDNIGRL PLHYAASQG HYQCVFTLV GIGSSTNAV 
      DMEGCTPLH LACGYDTEG KCIEYLLEH KSDPFVKDR 
      RGFTPIHYA LAGGNIAGV SRLLGVINN SMIFQEPDM 
      PDVTPLHLA AKLGNINVM RAILSYYHD VNLQTEQGL 
      TPLILAARE GHVQCVNIL LRFGAKVAL CDNVNGMTA 
      VHYSAKNGH SQSLTLLLH NSEDKNVID MHDGFKRTA 
      LMLAVSGNH IECVQTLLK CGADPNIVD DDKHSCLFR 
      AVVTGQNSM VQLLLSNNA KADSLDIYG KSVLHLAAA 
      CGHLVCLQT IVGYLTEKD TAVLDNQQC SALHWACYN 
      GNANCLEFL LENNVCEKM EGNPFSAAH CAAFAGSER 
      CLELLLHKF GPEITQLRD TRDRTPLHI AALHGHVEC 
      AKLIVEKGG DVKSCDEDG RTPLIAAAQ YGQVAFVEY 
      LLGCSGIDR TASDKQGNT ALHWACYRK YNNIALLLL 
      ENDDVGFVN LANNDGKTA LHLSSRNGL VDVTRELLQ 
      KGASVSAVD NEGLTPALC CAPNMNVAQ CLALILENL 
      PEFQLSKDG FRRQYNGHS TALDFHIHY EQ
    CDS sequence
    • >TC015680 RA
      atgggactg taccagttg gaggaacag ccccccatc 
      gtcaaggcc atcttcgcg ggctgcgag cccgagaaa 
      tacaaagaa ctgattaaa tcgaaacag gacgtaaac 
      tgccttgat agcaaaaag cgcactcct ctgcacgcg 
      gccgcgttc accggcgac gccgcaatc gcctccgtc 
      ttgctctcg aacggggcc cgagtcaac gcgaaagac 
      accaagtgg ctgacgccg ctccaccaa gcctgctac 
      atccgctca agcgagaca gtgtccatt ctgttgaac 
      aataacgcc gacgtcaac gcgcgagac aagctctgg 
      cagacgccc ctgcacgtg gcggcggcc aacggggcg 
      tacaaatgc atcgagcag ttgctcaac cacgtgccc 
      aaccccaac gtcaccgac agagggggg cgcacggcc 
      ctgcacctc gccgcctac agcgaaatg gccgattgc 
      gtggaactc ctcatttcg gggggctgt attgtcaac 
      gcgtacgac aagaaggac tgccgcccc ctgcaccgg 
      gcggtgcag gtgggcagc ttgagcata atcgagctg 
      ttgttaaaa tacaaagct gagattaac gctaaagac 
      aggaaccag tacaccccc ttgcatgtg gcggcggcc 
      gggggcaca gacgccgtg tgccgcctt ttgatcagt 
      cacggggct gacgtgaac gcgcagaat gtcttcggg 
      aacaccccc ttgcatatt gcgtgtctc aacggacac 
      catttggtc tgccaggag ttgatcaac tcgggggct 
      gatatcgaa gcggtgaat tatcggggg cagacgccc 
      ctgcatatc gctgcggtt agcacgaat ggggttgat 
      tgcatgatg ttgttattg acgcaaaag gttgatatt 
      aataggcag agtctcgat ggtaggact ccgctacat 
      atgacggca atacatggg aggtttacg aggagtaag 
      attttgatt gacaagggg gcgacgatt gattgtccc 
      gataagaat gattgtacc cccttgcat attgcggcg 
      aggtatggg cacgatttg ttgaccaat acattgttg 
      tcttatggg gctaatccc tcacagagg ggctacgaa 
      gggaggcag ccccttcat atgtgctgt ttggccggt 
      tatgtagag tgttgcagg aagttactg caagctggg 
      gtggattta aacgctgtg gatgattcg gggaaaacg 
      ccaactcat tgcgcagcg tataaaggt tcggtggaa 
      tgtttggat ttgcttgtg agcaacggt gccaagttt 
      caactaaaa gacaacatc ggccgtctt cccttgcac 
      tatgccgct tcccaaggc cactaccag tgtgttttc 
      acacttgtt ggtatcgga agttcgaca aatgctgtt 
      gacatggag ggctgtact cccctacac ttagcctgt 
      ggttacgac accgaaggc aaatgcatt gaatacctc 
      ctagaacac aaatccgat cctttcgtc aaggatcgg 
      cgtggtttc accccaatt cattatgcc ttggctgga 
      ggcaacata gctggagtt agccgcctg ttgggagtg 
      attaacaat tcgatgatt ttccaagag ccagacatg 
      cccgatgtg actccactc catctagcg gcaaaactg 
      ggcaatatt aacgtaatg cgtgcaatt ttgagttac 
      taccacgac gtgaatttg caaactgag cagggcttg 
      acgcctttg atcctcgca gcgagggag ggtcacgtg 
      cagtgcgtt aatatcctg ctcagattc ggtgccaaa 
      gtggctctc tgtgataac gttaacggc atgacggca 
      gtgcattac agtgccaaa aatggccat tcgcaatct 
      ctcactttg ctgttacac aacagcgaa gataaaaac 
      gtgattgat atgcacgat gggttcaaa cgaaccgct 
      ctaatgttg gcggtttcg gggaatcac atcgagtgc 
      gttcagact ctgttgaag tgtggagcc gatccgaat 
      attgtcgat gatgataaa cattcctgt ttgtttagg 
      gctgtagtt acagggcag aacagtatg gtgcaattg 
      ttattatca aataatgcg aaagcagat agtttggat 
      atttatggg aaaagtgtg ctacatctg gcggctgct 
      tgtggtcat cttgtttgc ttacaaacc attgttggt 
      tatctgacc gaaaaagat acagcggtc ttggataat 
      cagcagtgt tcagcgttg cattgggcg tgctataac 
      ggcaacgca aactgcctc gaattcctg ctggaaaac 
      aacgtttgc gagaaaatg gaaggcaac ccattctca 
      gccgctcat tgtgcagca ttcgcaggt tcggagcgt 
      tgtttagaa ctattattg cacaaattc ggaccggaa 
      ataacgcaa ttgcgggac acccgagac cgcacccct 
      ttgcacatc gccgccttg cacggacac gtcgagtgc 
      gcgaaatta atcgtggaa aaagggggc gatgttaag 
      agctgcgat gaggacgga aggacgccg ctaatcgca 
      gccgcgcaa tacggacaa gtggctttc gttgaatat 
      ttattgggt tgctcgggg attgacagg acggccagt 
      gacaagcag ggcaatacg gcgctgcat tgggcctgc 
      tatcgcaaa tataacaac atagcgttg ttgttgctc 
      gagaatgat gatgttggg tttgttaat cttgcaaat 
      aatgatggg aaaacggcg cttcatttg tcgtcccgc 
      aatggcttg gtggacgtg actagagaa ctgctccaa 
      aaaggggct tccgtgtcg gccgttgac aacgagggc 
      ttgacaccg gccttgtgc tgtgccccc aacatgaac 
      gtggctcaa tgtctagct ttaattctt gagaatttg 
      cccgaattt cagttatcc aaagacggt ttcagacgc 
      cagtacaat ggtcatagt acagcctta gattttcat 
      atacattat gaacaatga
    mRNA sequence
    • >TC015680 RA
      cgtagataa ttttaattt attccgcaa tggtttaac 
      cacttcccc cttatttca tgtttcgcc ttaagttgc 
      aagtaacta acaatggga ctgtaccag ttggaggaa 
      cagcccccc atcgtcaag gccatcttc gcgggctgc 
      gagcccgag aaatacaaa gaactgatt aaatcgaaa 
      caggacgta aactgcctt gatagcaaa aagcgcact 
      cctctgcac gcggccgcg ttcaccggc gacgccgca 
      atcgcctcc gtcttgctc tcgaacggg gcccgagtc 
      aacgcgaaa gacaccaag tggctgacg ccgctccac 
      caagcctgc tacatccgc tcaagcgag acagtgtcc 
      attctgttg aacaataac gccgacgtc aacgcgcga 
      gacaagctc tggcagacg cccctgcac gtggcggcg 
      gccaacggg gcgtacaaa tgcatcgag cagttgctc 
      aaccacgtg cccaacccc aacgtcacc gacagaggg 
      gggcgcacg gccctgcac ctcgccgcc tacagcgaa 
      atggccgat tgcgtggaa ctcctcatt tcggggggc 
      tgtattgtc aacgcgtac gacaagaag gactgccgc 
      cccctgcac cgggcggtg caggtgggc agcttgagc 
      ataatcgag ctgttgtta aaatacaaa gctgagatt 
      aacgctaaa gacaggaac cagtacacc cccttgcat 
      gtggcggcg gccgggggc acagacgcc gtgtgccgc 
      cttttgatc agtcacggg gctgacgtg aacgcgcag 
      aatgtcttc gggaacacc cccttgcat attgcgtgt 
      ctcaacgga caccatttg gtctgccag gagttgatc 
      aactcgggg gctgatatc gaagcggtg aattatcgg 
      gggcagacg cccctgcat atcgctgcg gttagcacg 
      aatggggtt gattgcatg atgttgtta ttgacgcaa 
      aaggttgat attaatagg cagagtctc gatggtagg 
      actccgcta catatgacg gcaatacat gggaggttt 
      acgaggagt aagattttg attgacaag ggggcgacg 
      attgattgt cccgataag aatgattgt acccccttg 
      catattgcg gcgaggtat gggcacgat ttgttgacc 
      aatacattg ttgtcttat ggggctaat ccctcacag 
      aggggctac gaagggagg cagcccctt catatgtgc 
      tgtttggcc ggttatgta gagtgttgc aggaagtta 
      ctgcaagct ggggtggat ttaaacgct gtggatgat 
      tcggggaaa acgccaact cattgcgca gcgtataaa 
      ggttcggtg gaatgtttg gatttgctt gtgagcaac 
      ggtgccaag tttcaacta aaagacaac atcggccgt 
      cttcccttg cactatgcc gcttcccaa ggccactac 
      cagtgtgtt ttcacactt gttggtatc ggaagttcg 
      acaaatgct gttgacatg gagggctgt actccccta 
      cacttagcc tgtggttac gacaccgaa ggcaaatgc 
      attgaatac ctcctagaa cacaaatcc gatcctttc 
      gtcaaggat cggcgtggt ttcacccca attcattat 
      gccttggct ggaggcaac atagctgga gttagccgc 
      ctgttggga gtgattaac aattcgatg attttccaa 
      gagccagac atgcccgat gtgactcca ctccatcta 
      gcggcaaaa ctgggcaat attaacgta atgcgtgca 
      attttgagt tactaccac gacgtgaat ttgcaaact 
      gagcagggc ttgacgcct ttgatcctc gcagcgagg 
      gagggtcac gtgcagtgc gttaatatc ctgctcaga 
      ttcggtgcc aaagtggct ctctgtgat aacgttaac 
      ggcatgacg gcagtgcat tacagtgcc aaaaatggc 
      cattcgcaa tctctcact ttgctgtta cacaacagc 
      gaagataaa aacgtgatt gatatgcac gatgggttc 
      aaacgaacc gctctaatg ttggcggtt tcggggaat 
      cacatcgag tgcgttcag actctgttg aagtgtgga 
      gccgatccg aatattgtc gatgatgat aaacattcc 
      tgtttgttt agggctgta gttacaggg cagaacagt 
      atggtgcaa ttgttatta tcaaataat gcgaaagca 
      gatagtttg gatatttat gggaaaagt gtgctacat 
      ctggcggct gcttgtggt catcttgtt tgcttacaa 
      accattgtt ggttatctg accgaaaaa gatacagcg 
      gtcttggat aatcagcag tgttcagcg ttgcattgg 
      gcgtgctat aacggcaac gcaaactgc ctcgaattc 
      ctgctggaa aacaacgtt tgcgagaaa atggaaggc 
      aacccattc tcagccgct cattgtgca gcattcgca 
      ggttcggag cgttgttta gaactatta ttgcacaaa 
      ttcggaccg gaaataacg caattgcgg gacacccga 
      gaccgcacc cctttgcac atcgccgcc ttgcacgga 
      cacgtcgag tgcgcgaaa ttaatcgtg gaaaaaggg 
      ggcgatgtt aagagctgc gatgaggac ggaaggacg 
      ccgctaatc gcagccgcg caatacgga caagtggct 
      ttcgttgaa tatttattg ggttgctcg gggattgac 
      aggacggcc agtgacaag cagggcaat acggcgctg 
      cattgggcc tgctatcgc aaatataac aacatagcg 
      ttgttgttg ctcgagaat gatgatgtt gggtttgtt 
      aatcttgca aataatgat gggaaaacg gcgcttcat 
      ttgtcgtcc cgcaatggc ttggtggac gtgactaga 
      gaactgctc caaaaaggg gcttccgtg tcggccgtt 
      gacaacgag ggcttgaca ccggccttg tgctgtgcc 
      cccaacatg aacgtggct caatgtcta gctttaatt 
      cttgagaat ttgcccgaa tttcagtta tccaaagac 
      ggtttcaga cgccagtac aatggtcat agtacagcc 
      ttagatttt catatacat tatgaacaa tgagggttt 
      gtgatcgat gatctttac acatttatc acaagttgc 
      tttttatat cgtatttta cctcttaat gtcactacc 
      aattagctg ctggtgagg tgctatttt ttacaacca 
      gttggaacc agtggccag tttgtgtac ttattttaa 
      ctcgtttta taactcaac tagtcgctt ttttctttg 
      ttataagaa acaaagtgt tcaaataac aattttggt 
      atgacttgt agtatttat tgcatttta cagttttta 
      ataaaatca tctcaaacg g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY