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Gene information
General information
TC015033 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid41: 689848..705615
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015033
      iBeetle screen iB_02821
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015033 RA
        MLHLFLLIS AVTVICDSF NPIYLNLSY SNFYTFSIN 
        KSVEYILEF SAPELKYPP RVTINSSDA QIKTPLMVV 
        ARQPKELLS WQLPMVLES DTGNHNFTK ISRTLCHDM 
        YRDYAPRGI RVDSPIVSV STAAPQNVT FTVQVDYQK 
        DFFIKPSVK YNFNITPSE PRFYFYNFT ANITESPNS 
        NYETVILEV FSDDFVCMT VSIQNASCP VFDTNQDIT 
        FRGFYETVN TQGGITIPK YKFPYGFFA VFVAKPDDS 
        DCTGIPSLY YDTNRTKTI TLIVKPSIS YQDYVNAVI 
        ATLSSIGIF YFVFIAGFI FCSKRGYVP RQMEYVSSE 
        PATPSTCLG EEVDEISLD ETEYDVVSE ADQDKSIRL 
        GKSVVYLSD LARKDPRVH KYKSYLYLY NVLTVALFY 
        GLPVIQLVV TYQRVLNET GQQDLCYYN FLCAHPLGV 
        ISDFNHVFS NSGYVLLGL LFLGITYRR EITHKDLNF 
        ERQYGIPQH YGMFYAMGV ALIMEGVLS GSYHVCPNT 
        ANFQFDSSF MYVMAVLCM VKLYQNRHP DINATAYAT 
        FGVLAVAIL LGMIGILEG NLYFWIVFT IIYLLSCFY 
        LSIQIYYMG CWKLDAGLA MRVWRICVY EFWSGPLNV 
        IKPIHKARM CLLIIANLC NWGMAFWGV YKHQKDFAL 
        FLLAIFMGN TLLYFSFYI VMKIINKER VNKLSLFFL 
        SLSVLCAIS AMYFFLNKS ISWSRTPAQ SRQFNQECK 
        LLRFYDFHD IWHFLSAIG MFFTFMVLL TLDDDLSHT 
        HRNKIVVF
      CDS sequence
      • >TC015033 RA
        atgttgcac cttttccta ctaatcagt gcagtgacc 
        gtaatttgt gatagtttc aatcccatt tacttgaac 
        ctgagctac agcaatttc tacacattt tccatcaac 
        aaatccgtt gaatacatc ctcgaattt tcagccccc 
        gagctaaaa tacccccca agagtgacg ataaacagc 
        agcgatgcc cagatcaaa accccccta atggtggtg 
        gcaagacag cccaaagaa ctgctatcg tggcaactg 
        cccatggtg ctcgaaagc gacaccggc aaccacaac 
        ttcacgaaa atatcgcgc actttgtgc cacgacatg 
        taccgggac tacgccccc cgaggaatc agggtcgac 
        agccccatc gtgagcgtg tcgacggcg gccccccag 
        aacgtgacc ttcacggtg caggtcgac taccagaag 
        gacttcttc atcaagcct tcagtcaag tacaacttc 
        aacataacc cccagcgag ccgcgattc tacttttac 
        aatttcacc gcaaacatc accgaaagc cccaactcc 
        aactacgag acggtcatt ctggaggtt ttctcggac 
        gatttcgtc tgtatgacc gtcagtatc cagaatgcg 
        agctgtccg gtgtttgac accaatcag gacatcacg 
        tttcgggga ttttacgag actgttaac acgcaaggg 
        ggaattacc atacctaaa tacaaattt ccgtacggc 
        tttttcgcc gtgttcgtg gcaaaaccg gacgactcc 
        gactgcacc ggcatccca tccctttac tacgacacc 
        aaccgcacc aagaccatc accctcatc gtgaagccg 
        agcatctcg taccaggac tacgtcaac gccgtcatc 
        gccaccctc tcctccatc ggcatcttc tacttcgtc 
        ttcatcgcc ggtttcatc ttctgctcg aaacgcggc 
        tacgtcccg cgccagatg gagtacgtg agcagcgag 
        ccggcgact cctagcacg tgcctcggc gaagaggtc 
        gacgaaatc tcgctcgat gagacggag tacgacgtc 
        gtctccgaa gccgatcag gataagtcg atccggttg 
        gggaaatct gtggtctat ttgagcgat ctggcgcgg 
        aaagaccca cgagtgcat aaatacaag tcgtatctg 
        tacttgtat aatgtcctg accgttgct ctgttctat 
        ggcttgccg gttatacag ctggtggtg acctatcag 
        agggttttg aacgagacg ggacagcag gatctgtgc 
        tactataat tttctgtgt gcacatcct ttgggggtt 
        atcagtgat tttaatcat gtcttctcg aatagtggg 
        tacgttctc ctggggttg ctgtttctc gggattacg 
        tacaggagg gaaattacg cacaaggat ctcaatttt 
        gaacggcaa tatggtatt ccgcaacat tatggcatg 
        ttctacgcc atgggcgtg gccctgatc atggaaggc 
        gtgctttcg gggagttac cacgtgtgc cccaacaca 
        gccaacttc caattcgat tctagtttc atgtacgtg 
        atggccgtc ctttgcatg gtcaaactc taccaaaac 
        cgtcatccg gacataaac gccacagct tatgccact 
        tttggggtg ttggcggtg gcgattctt ttagggatg 
        atcgggatt ttggaaggg aatctctac ttctggatc 
        gtcttcacc attatttat ttattgtcc tgtttttat 
        ctcagtatt caaatctac tacatgggg tgttggaaa 
        ttggacgcg ggtttggcg atgagagtg tggcgaatt 
        tgcgtttac gaattttgg tccgggcct ctaaatgtg 
        atcaaaccc attcataaa gcgagaatg tgtctcttg 
        attatcgca aatttgtgt aattggggg atggcgttt 
        tggggggtt tacaagcac caaaaggat tttgctctt 
        ttcctattg gctatcttc atgggcaac actttgttg 
        tatttcagc ttctatatt gttatgaag attattaac 
        aaggaaaga gtcaacaaa ttgtctctg ttcttcctg 
        tccctgtct gtactatgt gccatttct gccatgtat 
        tttttccta aacaaaagt atttcctgg tcgcgaact 
        ccagcgcaa tcgcgacag tttaaccaa gagtgcaaa 
        ctgttgcga ttttacgac ttccacgac atttggcac 
        tttttgagc gccattggt atgtttttc acatttatg 
        gttcttttg acattggac gatgacttg tcccacacc 
        catcggaac aaaattgtg gtattttaa
      mRNA sequence
      • >TC015033 RA
        acgaaccat cacgtgtgt gtctttcaa ccgcaaact 
        ctaaccttc aaatttgca ctaaaatga ccgaaaaaa 
        aacaatgac accaaaaat gttgcacct tttcctact 
        aatcagtgc agtgaccgt aatttgtga tagtttcaa 
        tcccattta cttgaacct gagctacag caatttcta 
        cacattttc catcaacaa atccgttga atacatcct 
        cgaattttc agcccccga gctaaaata ccccccaag 
        agtgacgat aaacagcag cgatgccca gatcaaaac 
        ccccctaat ggtggtggc aagacagcc caaagaact 
        gctatcgtg gcaactgcc catggtgct cgaaagcga 
        caccggcaa ccacaactt cacgaaaat atcgcgcac 
        tttgtgcca cgacatgta ccgggacta cgccccccg 
        aggaatcag ggtcgacag ccccatcgt gagcgtgtc 
        gacggcggc cccccagaa cgtgacctt cacggtgca 
        ggtcgacta ccagaagga cttcttcat caagccttc 
        agtcaagta caacttcaa cataacccc cagcgagcc 
        gcgattcta cttttacaa tttcaccgc aaacatcac 
        cgaaagccc caactccaa ctacgagac ggtcattct 
        ggaggtttt ctcggacga tttcgtctg tatgaccgt 
        cagtatcca gaatgcgag ctgtccggt gtttgacac 
        caatcagga catcacgtt tcggggatt ttacgagac 
        tgttaacac gcaaggggg aattaccat acctaaata 
        caaatttcc gtacggctt tttcgccgt gttcgtggc 
        aaaaccgga cgactccga ctgcaccgg catcccatc 
        cctttacta cgacaccaa ccgcaccaa gaccatcac 
        cctcatcgt gaagccgag catctcgta ccaggacta 
        cgtcaacgc cgtcatcgc caccctctc ctccatcgg 
        catcttcta cttcgtctt catcgccgg tttcatctt 
        ctgctcgaa acgcggcta cgtcccgcg ccagatgga 
        gtacgtgag cagcgagcc ggcgactcc tagcacgtg 
        cctcggcga agaggtcga cgaaatctc gctcgatga 
        gacggagta cgacgtcgt ctccgaagc cgatcagga 
        taagtcgat ccggttggg gaaatctgt ggtctattt 
        gagcgatct ggcgcggaa agacccacg agtgcataa 
        atacaagtc gtatctgta cttgtataa tgtcctgac 
        cgttgctct gttctatgg cttgccggt tatacagct 
        ggtggtgac ctatcagag ggttttgaa cgagacggg 
        acagcagga tctgtgcta ctataattt tctgtgtgc 
        acatccttt gggggttat cagtgattt taatcatgt 
        cttctcgaa tagtgggta cgttctcct ggggttgct 
        gtttctcgg gattacgta caggaggga aattacgca 
        caaggatct caattttga acggcaata tggtattcc 
        gcaacatta tggcatgtt ctacgccat gggcgtggc 
        cctgatcat ggaaggcgt gctttcggg gagttacca 
        cgtgtgccc caacacagc caacttcca attcgattc 
        tagtttcat gtacgtgat ggccgtcct ttgcatggt 
        caaactcta ccaaaaccg tcatccgga cataaacgc 
        cacagctta tgccacttt tggggtgtt ggcggtggc 
        gattctttt agggatgat cgggatttt ggaagggaa 
        tctctactt ctggatcgt cttcaccat tatttattt 
        attgtcctg tttttatct cagtattca aatctacta 
        catggggtg ttggaaatt ggacgcggg tttggcgat 
        gagagtgtg gcgaatttg cgtttacga attttggtc 
        cgggcctct aaatgtgat caaacccat tcataaagc 
        gagaatgtg tctcttgat tatcgcaaa tttgtgtaa 
        ttgggggat ggcgttttg gggggttta caagcacca 
        aaaggattt tgctctttt cctattggc tatcttcat 
        gggcaacac tttgttgta tttcagctt ctatattgt 
        tatgaagat tattaacaa ggaaagagt caacaaatt 
        gtctctgtt cttcctgtc cctgtctgt actatgtgc 
        catttctgc catgtattt tttcctaaa caaaagtat 
        ttcctggtc gcgaactcc agcgcaatc gcgacagtt 
        taaccaaga gtgcaaact gttgcgatt ttacgactt 
        ccacgacat ttggcactt tttgagcgc cattggtat 
        gtttttcac atttatggt tcttttgac attggacga 
        tgacttgtc ccacaccca tcggaacaa aattgtggt 
        attttaaat tattttgta taaataaat acgttaagg 
        taataaaaa ttaaaccag a