Status
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Gene information
General information
TC015037 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4304534..4316516
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015037
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02820

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015037 RA
      MSCDNDEAS RGCSPPNVV FEASTQTPT NEDCESPLF 
      TPKGYCLKT VEHSSLALG NIQKMKQQG QLCDITLKI 
      GLERFRAHK VILASVSPY FYAMFNGDM KEQHQSEIT 
      IHDLDPTAI DLLIEYSYT GQILITPDN VQVLLPASS 
      VLQIQEVRE ACCKFLLRQ LHPTNCLGI RSFADTHCC 
      KELHLKSHV YALQNFQQV VGTEEFLLL PVEEVKELI 
      SNSQLNISS EEDVFTAVL NWVKHDLGE RSRFISQLM 
      VHVRLPLVN REFLMTRVD NERLIRDDS ECRELLLEA 
      MRYHLAPER RCALSTSRT IERKPKGAD PYLFAVGGG 
      SLFAIHSEC EVYNPKSDT WSVIAPMLW RRSRSGVTG 
      LRRLLYVVG GYDGNSDLA TAECYNPLV NAWTPITPM 
      GTKRSCLGI CSFDGLIYV CGGYDGASC LSSMERYDP 
      LTGVWCSCP AMNTRRRYC RIAVVENCI YALGGFDST 
      NYQASVERF DPREGTWAP IPSMSSRRS SCGVVAYDG 
      HLYCIGGND GTTCMSSGE KFNVRRNAW EPIAAMHNR 
      RSTHEIVAM DGFIYALGG NDGSSSLNS VEKYDPKLN 
      KWTVVASMS IRRSSVGGA VLDCINLEY VLKQTMS
    CDS sequence
    • >TC015037 RA
      atgagttgc gacaacgac gaggcgtct cgcggatgc 
      agccccccg aatgtggtg ttcgaggcg tcgacgcaa 
      acccccacg aatgaggac tgcgagtcg ccgctgttc 
      acccccaag gggtactgt ctcaagacg gtggagcat 
      agcagtttg gcgctggga aatatccaa aaaatgaaa 
      cagcaagga cagctctgt gacataacg ttaaaaata 
      ggcttagag cgatttaga gcccataag gtgatctta 
      gcctcagtt agtccctac ttttacgcc atgttcaac 
      ggcgatatg aaagagcaa caccagagc gaaatcaca 
      atccatgac ctggacccc accgccatc gacctcctc 
      atcgagtac tcctacacg gggcagatc ctcatcacg 
      cccgacaac gtccaggtg ctcctcccc gccagcagc 
      gtcctccaa atccaggaa gtccgggag gcttgttgc 
      aaattcctc ctgagacag ctccatcca actaattgt 
      ctgggaata cggagcttc gccgacacg cattgctgc 
      aaagagttg catctcaag tcgcacgtc tacgccttg 
      cagaacttc cagcaggtg gtgggcacc gaggagttt 
      ctcctgctg ccggttgag gaggtcaag gagttgatt 
      tccaacagt cagttgaat atttcgtca gaggaggat 
      gtttttaca gcggttttg aattgggtg aagcatgac 
      ttgggggag aggagtcgg tttatctcg cagttaatg 
      gtccacgtg cgtctgccc ctcgtcaac cgcgagttc 
      ctcatgacg cgcgtggac aacgagcgc ctcatccgc 
      gacgacagc gagtgccgg gagctcctc ctcgaagcc 
      atgcgctac cacctggcc cccgagcgg cgctgcgcc 
      ttgtccacc tcccgcacc atcgagcgc aaaccgaag 
      ggtgccgac ccgtacctc ttcgccgtc ggcggcggc 
      tccctcttc gccatccac tccgaatgc gaggtgtac 
      aaccccaaa agcgacacc tggtccgtg atagccccc 
      atgctgtgg cggcgctcg cgctcgggc gtcaccggc 
      ctgcgccgc ctcctctac gtggtgggc ggctacgac 
      ggcaactcg gacctcgcc accgccgag tgctacaac 
      cccctggtc aacgcctgg acccccatc acgcccatg 
      ggcacgaag cggtcctgt ctggggatt tgctccttc 
      gatgggctg atttacgtg tgtgggggc tatgatggg 
      gcgtcgtgt ttgagttcg atggaacgg tacgacccc 
      ctgacgggg gtgtggtgc agttgtccg gcgatgaac 
      accaggagg cggtattgc aggatcgcg gtggtggag 
      aattgtatt tatgccttg ggggggttt gactccacg 
      aattatcag gcgtcggtg gagaggttt gatccgagg 
      gagggcaca tgggcgccc atcccctcc atgtccagt 
      aggcggagc agctgtggg gtggtggcc tatgatggg 
      catttgtac tgcattggg ggcaacgat gggacgacg 
      tgtatgtcg agtggggag aagtttaat gtgaggaga 
      aatgcatgg gagccaatc gctgcgatg cataatcgg 
      aggtccact catgaaatc gtggctatg gacggtttt 
      atttacgct ttggggggc aacgacggc tcttctagc 
      ctcaactct gttgaaaaa tacgacccg aagttgaac 
      aaatggacc gtggtcgcg tccatgtcc attcgccga 
      agctccgta ggaggcgct gttctcgac tgcattaac 
      ttagagtac gttttaaag caaacaatg agttga
    mRNA sequence
    • >TC015037 RA
      ttgactgac ttttaaata atggtgcgg tgttagcac 
      ggggagcgg gaaaatgag ttgcgacaa cgacgaggc 
      gtctcgcgg atgcagccc cccgaatgt ggtgttcga 
      ggcgtcgac gcaaacccc cacgaatga ggactgcga 
      gtcgccgct gttcacccc caaggggta ctgtctcaa 
      gacggtgga gcatagcag tttggcgct gggaaatat 
      ccaaaaaat gaaacagca aggacagct ctgtgacat 
      aacgttaaa aataggctt agagcgatt tagagccca 
      taaggtgat cttagcctc agttagtcc ctactttta 
      cgccatgtt caacggcga tatgaaaga gcaacacca 
      gagcgaaat cacaatcca tgacctgga ccccaccgc 
      catcgacct cctcatcga gtactccta cacggggca 
      gatcctcat cacgcccga caacgtcca ggtgctcct 
      ccccgccag cagcgtcct ccaaatcca ggaagtccg 
      ggaggcttg ttgcaaatt cctcctgag acagctcca 
      tccaactaa ttgtctggg aatacggag cttcgccga 
      cacgcattg ctgcaaaga gttgcatct caagtcgca 
      cgtctacgc cttgcagaa cttccagca ggtggtggg 
      caccgagga gtttctcct gctgccggt tgaggaggt 
      caaggagtt gatttccaa cagtcagtt gaatatttc 
      gtcagagga ggatgtttt tacagcggt tttgaattg 
      ggtgaagca tgacttggg ggagaggag tcggtttat 
      ctcgcagtt aatggtcca cgtgcgtct gcccctcgt 
      caaccgcga gttcctcat gacgcgcgt ggacaacga 
      gcgcctcat ccgcgacga cagcgagtg ccgggagct 
      cctcctcga agccatgcg ctaccacct ggcccccga 
      gcggcgctg cgccttgtc cacctcccg caccatcga 
      gcgcaaacc gaagggtgc cgacccgta cctcttcgc 
      cgtcggcgg cggctccct cttcgccat ccactccga 
      atgcgaggt gtacaaccc caaaagcga cacctggtc 
      cgtgatagc ccccatgct gtggcggcg ctcgcgctc 
      gggcgtcac cggcctgcg ccgcctcct ctacgtggt 
      gggcggcta cgacggcaa ctcggacct cgccaccgc 
      cgagtgcta caaccccct ggtcaacgc ctggacccc 
      catcacgcc catgggcac gaagcggtc ctgtctggg 
      gatttgctc cttcgatgg gctgattta cgtgtgtgg 
      gggctatga tggggcgtc gtgtttgag ttcgatgga 
      acggtacga ccccctgac gggggtgtg gtgcagttg 
      tccggcgat gaacaccag gaggcggta ttgcaggat 
      cgcggtggt ggagaattg tatttatgc cttgggggg 
      gtttgactc cacgaatta tcaggcgtc ggtggagag 
      gtttgatcc gagggaggg cacatgggc gcccatccc 
      ctccatgtc cagtaggcg gagcagctg tggggtggt 
      ggcctatga tgggcattt gtactgcat tgggggcaa 
      cgatgggac gacgtgtat gtcgagtgg ggagaagtt 
      taatgtgag gagaaatgc atgggagcc aatcgctgc 
      gatgcataa tcggaggtc cactcatga aatcgtggc 
      tatggacgg ttttattta cgctttggg gggcaacga 
      cggctcttc tagcctcaa ctctgttga aaaatacga 
      cccgaagtt gaacaaatg gaccgtggt cgcgtccat 
      gtccattcg ccgaagctc cgtaggagg cgctgttct 
      cgactgcat taacttaga gtacgtttt aaagcaaac 
      aatgagttg aatcaactc cttcatggt acaaaaatt 
      ctttccgtg caattatac gacgttctg tgataacta 
      aagaaccaa aacttgttt caggcaaat ttatatatt 
      tatatgacg cggtacgtg tccaatttc tggttattt 
      gagctttta catgtacga aatcatcga cttatagag 
      gatttgatt ttgaaaatg gcgccattg gaagtggcg 
      ccaaatttg aatttttct attagaatt atgcaacga 
      tgtagttta ttgtgcaca ggtttaaat gtttgattg 
      aacgaacaa tgcgtagct tggcctagc tttatttaa 
      acactgcca ttgttgtgt agcttgatg tcagaataa 
      aaccggttc gaagac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY