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Gene information
General information
TC034738 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid41: 182360..213334
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034738
    iBeetle screen iB_02819
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034738 RA
      MHRAKKPQI TDLNQHLIC KLCKGYFVD ATTIIECLH 
      SFCRSCIVK YLAANKYCP VCDVQVHKS KPLLNIRQD 
      RTLQDIVYK LVPRLFQNE VQRRRDFYK THPEAKPNL 
      EKILDNYQH IISPEESIS LTMCYYGCK ESSRYLRCP 
      AAVTIGHLQ KLIRAKYGL SDNHHVDIL YNQDCLNST 
      LTLIDVSYI YSWKRKTPL ELTYRIYEY APKKTEVKK 
      EEASEQNNN WKEVQLRIS ENGEMSIIQ DEASELVNN 
      NNYKPEIVT VVDKKLPEL NLIGENSAA STCVTFSSS 
      TTTKTVFST INSTKCSKI PTEFKKKKE NGGKSEKSL 
      QNAVEKLTQ NRAKEQAKV EEQKTPTTN GETEPADTA 
      TVSAEKVDE KRSA
    CDS sequence
    • >TC034738 RA
      atgcaccga gcgaaaaaa ccacaaatc accgatctg 
      aaccaacac ctgatttgt aaactgtgc aagggttat 
      ttcgtcgat gccaccacg attatcgag tgtttgcac 
      tctttttgc agaagttgc atcgttaag tatttggcg 
      gcgaataag tactgtccc gtgtgcgat gtgcaagta 
      cacaagtcg aaaccgctg cttaacatc aggcaagac 
      agaacttta caagatatc gtctacaaa ttggttcca 
      agactgttt caaaatgag gttcaaagg cgacgcgat 
      ttttacaaa acgcatcca gaggcgaaa cctaaccta 
      gaaaaaatc ctggataat taccagcac atcatcagt 
      ccagaggaa tcgatttcg ttgacaatg tgctattac 
      gggtgtaaa gaatcgtcg cgttatctg cgctgtccc 
      gccgctgta acaatcggg catttgcag aaattaatt 
      cgggccaag tacggtctc tctgataat catcatgta 
      gatattttg tacaatcaa gattgcctt aactcaacg 
      ttgacccta atcgacgtg tcttatatt tattcctgg 
      aagaggaaa acccccctg gagctaacc taccgaatt 
      tacgaatac gcgccgaaa aaaaccgaa gtgaagaaa 
      gaggaagca agcgaacaa aacaacaac tggaaggaa 
      gtccaattg aggataagc gagaacggc gaaatgtca 
      ataatccaa gacgaagcc tccgaactt gtaaataac 
      aacaactac aagccggag atcgtaaca gtcgtcgat 
      aaaaagtta cctgaactc aatctaatc ggtgaaaac 
      agtgcagcc tcaacctgt gtcacgttc agttcgtcc 
      acgaccacc aaaaccgtt tttagtaca atcaattcg 
      actaaatgc agtaagatc ccgaccgag ttcaaaaag 
      aagaaggaa aacggcggc aaaagcgag aagagttta 
      caaaacgct gtggaaaaa ttgactcaa aatcgtgca 
      aaagaacaa gcgaaagtc gaggagcaa aaaacgccg 
      actacgaac ggcgaaacc gaaccagca gacaccgcg 
      acggtaagt gcggaaaag gtcgacgag aaacgaagt 
      gcctga
    mRNA sequence
    • >TC034738 RA
      cgagctaat gcgtttgat ttggtggca ttttccgac 
      gcttgtgtc tcccgtttt gcgagagtt ttccacgcg 
      aaaaacgcc ccgacttgt ggaaacctt gaagggccg 
      cgggcaagt ggtggaaaa accggccga attggaaaa 
      ttcttggtg aaaatcggt gacgccatt ttcgctcca 
      tctgtcaaa tgtgagcaa ggtccgtct gtcaaataa 
      ttaatttag aagtaaaca aaaatattt gtgaaaatt 
      ggtggcatt tgctcggtg gtgtggcgt tttcgatgg 
      ttttgccga agccgggac gctggtctg gcgctggga 
      cgcgacgtg gggcttttt aggtcagcg cgccaacaa 
      tatgggcat attatggcg aaccccccg taccatcat 
      gtaaggagg tttatggga tagcttggg acgatgcac 
      cgagcgaaa aaaccacaa atcaccgat ctgaaccaa 
      cacctgatt tgtaaactg tgcaagggt tatttcgtc 
      gatgccacc acgattatc gagtgtttg cactctttt 
      tgcagaagt tgcatcgtt aagtatttg gcggcgaat 
      aagtactgt cccgtgtgc gatgtgcaa gtacacaag 
      tcgaaaccg ctgcttaac atcaggcaa gacagaact 
      ttacaagat atcgtctac aaattggtt ccaagactg 
      tttcaaaat gaggttcaa aggcgacgc gatttttac 
      aaaacgcat ccagaggcg aaacctaac ctagaaaaa 
      atcctggat aattaccag cacatcatc agtccagag 
      gaatcgatt tcgttgaca atgtgctat tacgggtgt 
      aaagaatcg tcgcgttat ctgcgctgt cccgccgct 
      gtaacaatc gggcatttg cagaaatta attcgggcc 
      aagtacggt ctctctgat aatcatcat gtagatatt 
      ttgtacaat caagattgc cttaactca acgttgacc 
      ctaatcgac gtgtcttat atttattcc tggaagagg 
      aaaaccccc ctggagcta acctaccga atttacgaa 
      tacgcgccg aaaaaaacc gaagtgaag aaagaggaa 
      gcaagcgaa caaaacaac aactggaag gaagtccaa 
      ttgaggata agcgagaac ggcgaaatg tcaataatc 
      caagacgaa gcctccgaa cttgtaaat aacaacaac 
      tacaagccg gagatcgta acagtcgtc gataaaaag 
      ttacctgaa ctcaatcta atcggtgaa aacagtgca 
      gcctcaacc tgtgtcacg ttcagttcg tccacgacc 
      accaaaacc gtttttagt acaatcaat tcgactaaa 
      tgcagtaag atcccgacc gagttcaaa aagaagaag 
      gaaaacggc ggcaaaagc gagaagagt ttacaaaac 
      gctgtggaa aaattgact caaaatcgt gcaaaagaa 
      caagcgaaa gtcgaggag caaaaaacg ccgactacg 
      aacggcgaa accgaacca gcagacacc gcgacggta 
      agtgcggaa aaggtcgac gagaaacga agtgcctga 
      tttagtttt tttcggtgc cttgatacc gattcactg 
      tagcttaaa agaaagaga aatttaaaa aaaaacacc 
      aatagggaa aaatctcct gtttaagga taatactct 
      gcttaaaat aatagtgta gaaatggtt actttactt 
      tataacgtg tgtttgtaa acgggtcgg tgtcggatt 
      ggcgtccat attttgtag cggtttttt gcgttgggg 
      aaattcatt aaatttgct ttcggaaaa tttcgacgg 
      ctgtgattt ttttttcat ctgtattaa aaatcaaat 
      gtcggtaca aaatatggg cttgacttg tttatagat 
      acctggaat ttttatatt aataatagt ttaagatat 
      tt