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Gene information
General information
TC015628 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3703426..3711316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015628
    iBeetle screen iB_02818

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule potentially decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head seta partially irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015628 RA
      MGPSKLKKK HFRVKHQKV KLFRANEPF LSVFMWGIN 
      HTINELMHV TIPVMLLPD DFRAYSKIK VDNHLFNKE 
      NMSSHFKVK EYCPLVFRN IRERFGIDD LDYKESLTR 
      SQPLPEDSS GKSGAKFYQ SFDKLFIIK TLTSEEVER 
      MHSFLKHYH PYIVERHGK TLLPQYLGM YRLTVDNAE 
      TYIVVTRNV FSNHLNIHR KFDLKGSTV DREASEKER 
      EKDLPTLKD NDFVNEQMK VYIGEEAKQ KLMDTLTAD 
      VEFLTKLHL MDYSLLLGI HEVERGEQE MARERELES 
      ENAGQESDE SESGSGLES RNFGFNTPP DSPNALAQF 
      MREHSLQYE GGIIPELDI YAIPSCESA PVKEIYFIA 
      IIDVLTHYG VKKQAAKAA KTVKYGSNV DGISTCDPE 
      QYGKRFIDF ISKAIE
    CDS sequence
    • >TC015628 RA
      atggggccg tcgaagctc aaaaagaaa cacttcagg 
      gtgaagcac cagaaagtc aagcttttt agggcgaat 
      gagccgttt ctgtcggtc ttcatgtgg gggattaac 
      cacacgatt aatgaactg atgcatgtc accatccct 
      gtcatgctt ctgccagac gattttcgg gcctattcc 
      aaaatcaaa gttgataat catctgttc aataaggag 
      aatatgtcc tcgcatttt aaggttaag gagtactgt 
      cctttggtg tttaggaat atcagggag aggtttgga 
      attgatgat ttagactat aaggaatct ctgactaga 
      tcgcaaccc ttgccggaa gactcaagt ggcaaaagt 
      ggcgccaag ttttaccaa agtttcgac aaactcttc 
      atcattaaa acactgact tctgaagag gtcgagagg 
      atgcattcg ttcctcaaa cattatcat ccgtatatt 
      gtcgagaga cacggcaaa accctcttg ccccaatac 
      ctcggaatg taccgtcta acagtcgac aatgctgaa 
      acctacatt gtcgtaacc cgaaacgtt ttctcaaat 
      catttaaac atccatcga aaattcgac ctgaaaggt 
      tcgacagtc gaccgcgaa gcgtccgaa aaagaacgc 
      gaaaaagac cttcccacg ctcaaagat aacgatttc 
      gtcaacgaa caaatgaaa gtttacata ggcgaagag 
      gccaagcag aaattgatg gatacgtta acggccgat 
      gtggaattt ttgaccaaa ttgcatttg atggattat 
      agtcttttg cttggaatc cacgaggtg gagagaggc 
      gagcaagaa atggcaagg gaaagagaa ttagagtcg 
      gaaaatgcc ggacaagag tcggacgaa agtgagagt 
      ggatcgggt ttagaaagt cgaaatttt gggtttaat 
      acgccgcca gattcgccg aacgcactt gctcagttc 
      atgcgagaa catagtctg caatatgaa ggtgggata 
      atccccgaa ctggacatc tatgccata cccagctgc 
      gagtcagcc cccgtgaag gagatctac tttatcgcc 
      ataatcgac gtacttacc cattacggc gtaaaaaag 
      caggcagca aaggccgcc aaaacggtg aaatacggc 
      tcaaatgtc gacggaatc agtacctgc gatccggaa 
      cagtacggc aagcgattt atcgacttc ataagcaaa 
      gcaatcgaa tag
    mRNA sequence
    • >TC015628 RA
      actaatcaa tgaaggttt tcgggatgg ttagcacct 
      aaggttgag actggactg gcggccatt ttgcgaacc 
      agatgatgt ctttgccga gcagcatgg ggccgtcga 
      agctcaaaa agaaacact tcagggtga agcaccaga 
      aagtcaagc tttttaggg cgaatgagc cgtttctgt 
      cggtcttca tgtggggga ttaaccaca cgattaatg 
      aactgatgc atgtcacca tccctgtca tgcttctgc 
      cagacgatt ttcgggcct attccaaaa tcaaagttg 
      ataatcatc tgttcaata aggagaata tgtcctcgc 
      attttaagg ttaaggagt actgtcctt tggtgttta 
      ggaatatca gggagaggt ttggaattg atgatttag 
      actataagg aatctctga ctagatcgc aacccttgc 
      cggaagact caagtggca aaagtggcg ccaagtttt 
      accaaagtt tcgacaaac tcttcatca ttaaaacac 
      tgacttctg aagaggtcg agaggatgc attcgttcc 
      tcaaacatt atcatccgt atattgtcg agagacacg 
      gcaaaaccc tcttgcccc aatacctcg gaatgtacc 
      gtctaacag tcgacaatg ctgaaacct acattgtcg 
      taacccgaa acgttttct caaatcatt taaacatcc 
      atcgaaaat tcgacctga aaggttcga cagtcgacc 
      gcgaagcgt ccgaaaaag aacgcgaaa aagaccttc 
      ccacgctca aagataacg atttcgtca acgaacaaa 
      tgaaagttt acataggcg aagaggcca agcagaaat 
      tgatggata cgttaacgg ccgatgtgg aatttttga 
      ccaaattgc atttgatgg attatagtc ttttgcttg 
      gaatccacg aggtggaga gaggcgagc aagaaatgg 
      caagggaaa gagaattag agtcggaaa atgccggac 
      aagagtcgg acgaaagtg agagtggat cgggtttag 
      aaagtcgaa attttgggt ttaatacgc cgccagatt 
      cgccgaacg cacttgctc agttcatgc gagaacata 
      gtctgcaat atgaaggtg ggataatcc ccgaactgg 
      acatctatg ccataccca gctgcgagt cagcccccg 
      tgaaggaga tctacttta tcgccataa tcgacgtac 
      ttacccatt acggcgtaa aaaagcagg cagcaaagg 
      ccgccaaaa cggtgaaat acggctcaa atgtcgacg 
      gaatcagta cctgcgatc cggaacagt acggcaagc 
      gatttatcg acttcataa gcaaagcaa tcgaataga 
      tcgccgggg ggaaacagt tcttgcttt tttctcgtt 
      aacgttgtg ttgataatt gtaaatatg atattttag 
      atatgtaca taaacgatg tgatgaaga tcagttttt 
      aagttgttg atagtcaat tcggatgca gtcgtgttt 
      tcctgctag cactgcatt ggtctcttt cttattcac 
      acggaggaa agtcaatgt ttcattagc agtattata 
      aggccccca gtttttcat taggacaac agaatcaat 
      gtattaagt ggttaaatg ggaacgaat ttattagtt 
      ttgtcgaaa aatttggct tgactgatt agatttcgc 
      cgagttaga actacgtta aggcttcta ttgagtttt 
      cgtttgggt ttaagacaa taatgactt gtctcaaaa 
      aatggaaag gatgtaata tcttaatac agtttttac 
      actttttat atcgagttt ttactactt ttaaatgaa 
      tttcttgtc tgcgctgcg ccggttttt cgatggtat 
      acgatcatt gggattagt tttgccaaa tgagcttta 
      atatctata cttgttatt ttgtattta aattttata 
      tctagtcta tgttattat tgattattg ctatggtaa 
      tactttgta ataaatttt caattttca ctatcagta 
      taattaagg taacttgtc tttatatgt atcagctgt 
      gtttttttc ataataaat caagaacaa aact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY