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Gene information
General information
TC015081 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3595842..3643047
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015081
    iBeetle screen iB_02816

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    ovary swollen - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02817

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015081 RA
      MSAKSSNVE KLVRVGYYE LEKTIGKGN FAVVKLATH 
      IVTRTKVAI KIIDKTALD EENLTKIFR ETAILKKLR 
      HPHITRLYQ LMETNQTIY MVTEYASNG EIFDHLVAK 
      GRMPEDEAK RIFSQIVSA VSYCHSQGV VHRDLKAEN 
      LLLDHNLNI KLADFGFSN QFTEGCLLS TWCGSPPYA 
      APELFQGHK YDGPKADIW SLGVVLYVL VCGSLPFDG 
      RTLHDLRNV VIEGKFRIP YFMSQDCEY LIRHMLVVD 
      PEKRLTMSQ IAKHRWLAH TPPVDTGPE RELQLNKTV 
      IDHMLQLPN LNQNMVLAS VKNNTFDHI HAIYNLLLD 
      KLHQRTINF QSKISQQRR AGEELTLDY LHTRPPKIN 
      ERSESFNEQ QLVTHLAGE KRQAAEQQI DESFNYRRE 
      SFNENCLRR DEERRKSVN ESISEDGGS PFVSMPTIP 
      AVYLVDDGE NQPLEKFKE MDQDASEEA CSLSVPSTS 
      TGYGSCGSS GDKYLTVRR HTVGPGDSA HEQVRYMQG 
      RGNSKRHTM ANPEDVHTL QSGGGSGGR TRRTGLLTV 
      MERPPVIPP EVVMEVEAR MKRNYMPPM LPQRKHSRH 
      AVKPHLPTV QELQGREPK SMERFSPVR RGSEGSAAG 
      SRTLSPSTP QQECQRLQR GLQARSSPP RSIPGSPIH 
      QLVSDPQMR HQMAECSSP IHQFYPNDP ARDPTLGLP 
      PESYQSSYD PNRSFGNSP LHTSPFGTN PNSNLSSPI 
      QQNLFATNL TMYSGTNSP IMNPLLSPS TSPVFGNYV 
      QTPTAPGSI SSITQGISG LNTTGTGSI TQGTPSTNL 
      QLEIRNQQM QEDKMDTTY ATNLTSSFQ PNFLHQPHH 
      MHHILNIHN HRSLTNSPI SNPGSPGLD MIQEELPHV 
      HNIPKPDQQ TIGHPQISL TDVLGSEVT LVAGSDTSE 
      DSMDSLDNH KIPKIPSFI ISEPSENLP SITKGIGRK 
      TSQENDEAK FFTKPHETE PSDEFFLRR NSDKSSCYS 
      DDSLSNDSL SIGNQSPSS SSNTQSSHV CDNTFEKMQ 
      ITHELSDSG TGNFQIFQN LKLDDCNSN PEGGTNLHK 
      NSGSFEFEL SEVCSKLQS TDILEMVKR TITDQIPPK 
      TCSSSEDVS DRLSLEYEG GIQIELKVI DKQKDSKGL 
      KMRRISGDH LVYNQLCQQ LISCMTVS
    CDS sequence
    • >TC015081 RA
      atgtccgcg aaatcgtcg aacgttgaa aaattagtg 
      cgtgtcggt tattacgaa ctcgaaaaa accatcgga 
      aagggtaat ttcgccgtt gtcaagctg gccacacac 
      attgtcacc agaaccaag gtggccatt aaaataata 
      gataagact gctttagat gaagaaaac ttaacaaaa 
      atatttcga gagactgcc attctgaaa aaactaaga 
      catccacac attaccagg ctgtatcag cttatggag 
      acgaatcag acgatatac atggtgaca gagtatgcc 
      agtaacggg gaaattttt gatcacttg gtggcgaag 
      ggccgaatg cctgaagac gaagccaaa cggatattt 
      agtcaaatc gtttcggcc gtaagctac tgtcattct 
      cagggggta gttcataga gatttaaag gctgaaaac 
      ttattgttg gaccataat ttaaacatc aagcttgcc 
      gatttcggc ttcagtaat cagttcaca gagggctgt 
      cttttgtca acgtggtgt ggatcgccg ccttatgca 
      gctcctgaa ctatttcaa ggccataaa tatgatgga 
      cctaaagcc gatatatgg agtttgggt gtagtttta 
      tacgttttg gtatgtggt tccctccct ttcgacggt 
      cgaacattg catgacctt cgcaatgtg gtgattgaa 
      ggcaaattc cggattccg tacttcatg tcacaagat 
      tgcgaatat ttaataaga cacatgctg gtggtcgac 
      ccggaaaaa agactgacg atgtctcag atcgcgaaa 
      caccgatgg ctggcgcac acgcccccc gtcgacacc 
      ggtcctgag cgcgaactc caacttaac aagactgtg 
      atagaccac atgctgcaa ctgcccaac ctgaaccag 
      aacatggtc ctcgcctcc gtcaagaac aacaccttc 
      gaccacatc cacgccatc tacaatctg ctcttggac 
      aagctgcac cagcgcaca atcaacttc cagtcgaag 
      atctcgcag cagcgccgc gccggggaa gaactcact 
      ctggactat ctccacact cggccgccg aaaatcaac 
      gagcgcagc gagtcgttc aacgagcag cagctggtc 
      acgcatctc gcgggggag aagcgacaa gcggccgag 
      cagcaaatc gacgagtcg tttaattat cgacgggag 
      agtttcaat gagaattgt ttgaggcgg gatgaggag 
      aggcggaag tcggtcaat gagagtatt tcggaggat 
      ggggggtcg ccgtttgtg tcgatgccg accattccg 
      gcggtttac ctggtggac gatggggag aaccaaccg 
      ttggagaag ttcaaggag atggatcaa gatgcgtcg 
      gaggaggcg tgttcgttg agtgtgccg tcgacttcg 
      acgggttat ggcagttgt gggtcctcc ggtgataag 
      tatctgacg gtgaggcgg cacacggtt ggacccgga 
      gattcggca catgaacag gtcagatat atgcaaggg 
      aggggcaat tcgaagcgc cacacgatg gcgaacccc 
      gaagacgtc cacacctta caatcgggg ggcggttcc 
      ggggggcgc acccgccgt acgggcctc ctcaccgtc 
      atggagcga cctccagta atcccaccc gaagtagtg 
      atggaagtg gaagcacgc atgaagcgt aactacatg 
      cctccaatg ttaccacaa cgcaagcat tcgcggcac 
      gctgtcaaa ccacacttg cccacagta caagagctg 
      caaggtcgt gaacccaaa tccatggag aggttcagt 
      ccggtgcgg agaggcagc gagggctcg gcggcaggc 
      tcgaggact ttgagtcct tcgacgccc cagcaagag 
      tgccagagg ctgcagaga gggctgcag gcgaggtcg 
      agcccgccg aggtcgatt ccaggttcg cccattcac 
      cagctcgtg tccgacccg caaatgcgc caccagatg 
      gcggaatgt tcgtcaccg attcatcaa ttttaccca 
      aacgatccc gcccgcgat cccacattg ggacttccc 
      ccagaatcc taccaaagc tcctacgat ccgaatcgt 
      agtttcgga aacagtcct ttacacacg tccccgttc 
      ggtacgaac ccgaacagt aaccttagt tcaccgatt 
      caacagaat ctgttcgct accaaccta accatgtat 
      tctggtacc aactcgccc attatgaac ccattacta 
      agtcccagt acgtctcca gtttttggt aattatgtg 
      caaactccc acagcgcct ggaagcatc tcgtccata 
      actcaagga ataagtggt ttaaacacc accggaacc 
      ggttccata acccaaggc accccatcc accaacctc 
      cagttggaa atccgcaac caacaaatg caagaggac 
      aaaatggac accacctac gccacaaac ctcaccagt 
      agtttccag cccaacttc ctccaccag ccgcaccac 
      atgcatcac atcttgaac atccacaac catcgcagt 
      ttgaccaac tcgcccatc agcaatccg ggcagtccc 
      ggcctggac atgatccag gaggagctc ccccacgtc 
      cacaacatc cccaaaccc gaccagcag accatcggc 
      cacccgcag atctccctc acggacgtc ctcggcagc 
      gaggtgacc ctcgtcgcc ggctcggac acctccgag 
      gactccatg gacagtctc gacaaccac aaaatcccc 
      aaaatcccc tcgttcata atctcggaa ccctccgaa 
      aatctccca tcgatcacg aaaggcatc ggccgcaaa 
      acgagccag gaaaacgac gaggcgaaa tttttcacc 
      aaaccccac gagacggag ccgagtgac gagtttttt 
      ctaagacgc aattcggat aaaagttcg tgttattcc 
      gacgattcg ctctcgaac gacagtctc agcatcggg 
      aaccagagt ccgagctcc agctccaac acgcagagc 
      agtcacgtg tgcgacaac acgttcgaa aaaatgcaa 
      atcacgcac gagttgagc gactcggga actggcaat 
      ttccagatt ttccagaac ttgaagctg gacgattgc 
      aactcgaac ccggagggc gggacgaac ttgcacaaa 
      aactccggg tcgtttgag ttcgaattg agcgaagtg 
      tgttcgaag ctgcagtcg accgatatc ctggagatg 
      gtcaagcgg acgatcacc gaccaaatc ccgccgaag 
      acgtgctcg agttcggag gatgtgagc gatagattg 
      tcgttggag tacgagggc gggattcag atcgagctg 
      aaagtcatc gataagcaa aaagactcg aaaggtttg 
      aaaatgcga cgcatttcc ggcgatcat ttggtttat 
      aaccagctt tgtcaacag ctgatttcg tgtatgacg 
      gtttcctag
    mRNA sequence
    • >TC015081 RA
      aatcaacgt actaaaatc tagtagcct gtagaatat 
      tatttggtg acgagctga caccaaaat acggtgaaa 
      aatgtccgc gaaatcgtc gaacgttga aaaattagt 
      gcgtgtcgg ttattacga actcgaaaa aaccatcgg 
      aaagggtaa tttcgccgt tgtcaagct ggccacaca 
      cattgtcac cagaaccaa ggtggccat taaaataat 
      agataagac tgctttaga tgaagaaaa cttaacaaa 
      aatatttcg agagactgc cattctgaa aaaactaag 
      acatccaca cattaccag gctgtatca gcttatgga 
      gacgaatca gacgatata catggtgac agagtatgc 
      cagtaacgg ggaaatttt tgatcactt ggtggcgaa 
      gggccgaat gcctgaaga cgaagccaa acggatatt 
      tagtcaaat cgtttcggc cgtaagcta ctgtcattc 
      tcagggggt agttcatag agatttaaa ggctgaaaa 
      cttattgtt ggaccataa tttaaacat caagcttgc 
      cgatttcgg cttcagtaa tcagttcac agagggctg 
      tcttttgtc aacgtggtg tggatcgcc gccttatgc 
      agctcctga actatttca aggccataa atatgatgg 
      acctaaagc cgatatatg gagtttggg tgtagtttt 
      atacgtttt ggtatgtgg ttccctccc tttcgacgg 
      tcgaacatt gcatgacct tcgcaatgt ggtgattga 
      aggcaaatt ccggattcc gtacttcat gtcacaaga 
      ttgcgaata tttaataag acacatgct ggtggtcga 
      cccggaaaa aagactgac gatgtctca gatcgcgaa 
      acaccgatg gctggcgca cacgccccc cgtcgacac 
      cggtcctga gcgcgaact ccaacttaa caagactgt 
      gatagacca catgctgca actgcccaa cctgaacca 
      gaacatggt cctcgcctc cgtcaagaa caacacctt 
      cgaccacat ccacgccat ctacaatct gctcttgga 
      caagctgca ccagcgcac aatcaactt ccagtcgaa 
      gatctcgca gcagcgccg cgccgggga agaactcac 
      tctggacta tctccacac tcggccgcc gaaaatcaa 
      cgagcgcag cgagtcgtt caacgagca gcagctggt 
      cacgcatct cgcggggga gaagcgaca agcggccga 
      gcagcaaat cgacgagtc gtttaatta tcgacggga 
      gagtttcaa tgagaattg tttgaggcg ggatgagga 
      gaggcggaa gtcggtcaa tgagagtat ttcggagga 
      tggggggtc gccgtttgt gtcgatgcc gaccattcc 
      ggcggttta cctggtgga cgatgggga gaaccaacc 
      gttggagaa gttcaagga gatggatca agatgcgtc 
      ggaggaggc gtgttcgtt gagtgtgcc gtcgacttc 
      gacgggtta tggcagttg tgggtcctc cggtgataa 
      gtatctgac ggtgaggcg gcacacggt tggacccgg 
      agattcggc acatgaaca ggtcagata tatgcaagg 
      gaggggcaa ttcgaagcg ccacacgat ggcgaaccc 
      cgaagacgt ccacacctt acaatcggg gggcggttc 
      cggggggcg cacccgccg tacgggcct cctcaccgt 
      catggagcg acctccagt aatcccacc cgaagtagt 
      gatggaagt ggaagcacg catgaagcg taactacat 
      gcctccaat gttaccaca acgcaagca ttcgcggca 
      cgctgtcaa accacactt gcccacagt acaagagct 
      gcaaggtcg tgaacccaa atccatgga gaggttcag 
      tccggtgcg gagaggcag cgagggctc ggcggcagg 
      ctcgaggac tttgagtcc ttcgacgcc ccagcaaga 
      gtgccagag gctgcagag agggctgca ggcgaggtc 
      gagcccgcc gaggtcgat tccaggttc gcccattca 
      ccagctcgt gtccgaccc gcaaatgcg ccaccagat 
      ggcggaatg ttcgtcacc gattcatca attttaccc 
      aaacgatcc cgcccgcga tcccacatt gggacttcc 
      cccagaatc ctaccaaag ctcctacga tccgaatcg 
      tagtttcgg aaacagtcc tttacacac gtccccgtt 
      cggtacgaa cccgaacag taaccttag ttcaccgat 
      tcaacagaa tctgttcgc taccaacct aaccatgta 
      ttctggtac caactcgcc cattatgaa cccattact 
      aagtcccag tacgtctcc agtttttgg taattatgt 
      gcaaactcc cacagcgcc tggaagcat ctcgtccat 
      aactcaagg aataagtgg tttaaacac caccggaac 
      cggttccat aacccaagg caccccatc caccaacct 
      ccagttgga aatccgcaa ccaacaaat gcaagagga 
      caaaatgga caccaccta cgccacaaa cctcaccag 
      tagtttcca gcccaactt cctccacca gccgcacca 
      catgcatca catcttgaa catccacaa ccatcgcag 
      tttgaccaa ctcgcccat cagcaatcc gggcagtcc 
      cggcctgga catgatcca ggaggagct cccccacgt 
      ccacaacat ccccaaacc cgaccagca gaccatcgg 
      ccacccgca gatctccct cacggacgt cctcggcag 
      cgaggtgac cctcgtcgc cggctcgga cacctccga 
      ggactccat ggacagtct cgacaacca caaaatccc 
      caaaatccc ctcgttcat aatctcgga accctccga 
      aaatctccc atcgatcac gaaaggcat cggccgcaa 
      aacgagcca ggaaaacga cgaggcgaa atttttcac 
      caaacccca cgagacgga gccgagtga cgagttttt 
      tctaagacg caattcgga taaaagttc gtgttattc 
      cgacgattc gctctcgaa cgacagtct cagcatcgg 
      gaaccagag tccgagctc cagctccaa cacgcagag 
      cagtcacgt gtgcgacaa cacgttcga aaaaatgca 
      aatcacgca cgagttgag cgactcggg aactggcaa 
      tttccagat tttccagaa cttgaagct ggacgattg 
      caactcgaa cccggaggg cgggacgaa cttgcacaa 
      aaactccgg gtcgtttga gttcgaatt gagcgaagt 
      gtgttcgaa gctgcagtc gaccgatat cctggagat 
      ggtcaagcg gacgatcac cgaccaaat cccgccgaa 
      gacgtgctc gagttcgga ggatgtgag cgatagatt 
      gtcgttgga gtacgaggg cgggattca gatcgagct 
      gaaagtcat cgataagca aaaagactc gaaaggttt 
      gaaaatgcg acgcatttc cggcgatca tttggttta 
      taaccagct ttgtcaaca gctgatttc gtgtatgac 
      ggtttccta gcatttaac gaaattctc ttagtttca 
      ttacgcttt taagttaat ctcgaattg agtttttgg 
      ttcggttta cggattact agagattag gggcgaaac 
      gactgcaaa ttttgttta acgagcgta atgtttgcc 
      ataacgagt gagcgctgc gaggtatgc gaacaccta 
      ataccgttt atttcgggc ggcttgaac tgaataata 
      ataaaagag aaaacaaaa atcgtttat ttatacaaa 
      aagttaagt aaataaaaa taaacagga cagataata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY