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Gene information
General information
TC011477 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6838272..6863247
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011477
    iBeetle screen iB_02814

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: 50% are affected.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05043

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    elytra surface irregular - Number of animals showing the phenotype: 7

    hindwing folding irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011477 RA
      MDSRIGLDF IVENPEYIR KLAAALDTS NVTVKKQVF 
      ELLSALCVH NEEGHARAL DTLEHYKKL KGERYRLNV 
      IVQELSRAT AVDYQTALV AFINCLIIS TPRLTDRIR 
      RRNEFIGCH LLPVLNNLR KCAEAEPEL AVQLDVFDE 
      QRDSDDSQS LQGPHGVDL NSPLDVFYA ILKQVAETP 
      QEIPFLSIL QHLLRIDAK DAVSDIIWD TAERLVHRA 
      TLLENREDA ARLLRAPSA QSKFFCHCH LRSESSPTR 
      RPSLNTPQS PTPALPPPP PPPPNVPAV PPPVPAPPL 
      KPAAPLPPP ECDSVIEKL PQQEIPVPK IKMKTFNWN 
      KIPNNKVIG KNNIWTQVA YTHQHSPMA DLDWLEMEG 
      LFCQQAPPA NAAASFRRE NTNSETLDR RIRKENCEI 
      TLLDGKRSL NVNIFLKQF RSSNEDIIH LIREGEHDD 
      IGAEKLKGL LKILPEVDE LDMLKSFDG DFTKLGNAE 
      KFLIQLTNL SNYKLRIES MLLKEEFAS NMSYLEPSI 
      KSMIMAAQD LMTNKPLQE VLYMILVAG NFLNAGGYA 
      GNAAGVKLS SLQKITDIR ANKPNMNLI HFVALQAEK 
      KNPKLLTFT DNISVLEDA AKTTVEQLQ NEINVLDVR 
      IKKIKKQIE LATTEPEIK EQMTEFLQM AEREVSNLQ 
      QNMEELEKV RVQLAEFFC EDTNSFKLE ECFRIFHGF 
      YCKFKQAVG DNERRRIQE EQANARRRQ REELLASKR 
      RQMENLGGT PDSDVPFID LQIYDSRHG FPMKKGWKT 
      TNGTSEDEL SSVTGSPSL SRRRLGSFN GDPEKSPDI 
      TPNGSLRRR RSRVLSEED EGNLMDFLR ASDNNRERR 
      SWGSLDRSW ARKARGGSQ RKRPALLGA DFTSDRERP 
      SSPSPLSES KPIVSTPEE ETNKPKELR QKIESWLQA 
      SESDQTEDT RRQTRRITN RRSFEMDSE SERSSTLDT 
      LPEGKQVYS GSQSTYRKA YPAWEPSKT IDNTDVVSA 
      MEAVEEVQP QIKDKSAWR KSTLNVANS TEETKDDSM 
      RLNRLPVVE NDRKTLISS LGERSPTDK LTLYIIKPR 
      PRDSPPSLD DPPISNKVE IDQDNIETP PATRKIFIP 
      TPTEKREPT QPSPCRRLM GRPRDTDEP EPNPTLGDG 
      QFDRFSAAR RTRRYKRTD TDPDEPKDE PETRLKAWQ 
      DKLQNPGRR HRHQTGINQ DEVKLALTK TDKKNEDEG 
      FEESQSLMS ESPSQGASS GGNYEPEDS ALVVYAKPL 
      KSEPPVEKP AKGRGLERG GVRRTVQDT PKKSVIPRR 
      SGSLRKTNT STNVQRSSS RNSIVSSKS SLNSSASAC 
      TVKKMPVKA GVGKAPPPK PVKRTPSTG GTAGTRPPR 
      PLAMSFMKP TTSSATKVH TVPKNGAFR TK
    • >TC011477 RB
      MIAHNRTCP THTTKWKRP KSSEVKVSA STPSRSVRD 
      AQLSAAPLF KSPPPPNHT TYARPKRKG CFLASAASR 
      RPRKVPLEP TVRALFVNE SACAKGGKL HFSHVCRLG 
      CIRNNRTMD SRIGLDFIV ENPEYIRKL AAALDTSNV 
      TVKKQVFEL LSALCVHNE EGHARALDT LEHYKKLKG 
      ERYRLNVIV QELSRATAV DYQTALVAF INCLIISTP 
      RLTDRIRRR NEFIGCHLL PVLNNLRKC AEAEPELAV 
      QLDVFDEQR DSDDSQSLQ GPHGVDLNS PLDVFYAIL 
      KQVAETPQE IPFLSILQH LLRIDAKDA VSDIIWDTA 
      ERLVHRATL LENREDAAR LLRAPSAQS KFFCHCHLR 
      SESSPTRRP SLNTPQSPT PALPPPPPP PPNVPAVPP 
      PVPAPPLKP AAPLPPPEC DSVIEKLPQ QEIPVPKIK 
      MKTFNWNKI PNNKVIGKN NIWTQVAYT HQHSPMADL 
      DWLEMEGLF CQQAPPANA AASFRRENT NSETLDRRI 
      RKENCEITL LDGKRSLNV NIFLKQFRS SNEDIIHLI 
      REGEHDDIG AEKLKGLLK ILPEVDELD MLKSFDGDF 
      TKLGNAEKF LIQLTNLSN YKLRIESML LKEEFASNM 
      SYLEPSIKS MIMAAQDLM TNKPLQEVL YMILVAGNF 
      LNAGGYAGN AAGVKLSSL QKITDIRAN KPNMNLIHF 
      VALQAEKKN PKLLTFTDN ISVLEDAAK TTVEQLQNE 
      INVLDVRIK KIKKQIELA TTEPEIKEQ MTEFLQMAE 
      REVSNLQQN MEELEKVRV QLAEFFCED TNSFKLEEC 
      FRIFHGFYC KFKQAVGDN ERRRIQEEQ ANARRRQRE 
      ELLASKRRQ MENLGGTPD SDVPFIDLQ IYDSRHGFP 
      MKKGWKTTN GTSEDELSS VTGSPSLSR RRLGSFNGD 
      PEKSPDITP NGSLRRRRS RVLSEEDEG NLMDFLRAS 
      DNNRERRSW GSLDRSWAR KARGGSQRK RPALLGADF 
      TSDRERPSS PSPLSESKP IVSTPEEET NKPKELRQK 
      IESWLQASE SDQTEDTRR QTRRITNRR SFEMDSESE 
      RSSTLDTLP EGKQVYSGS QSTYRKAYP AWEPSKTID 
      NTDVVSAME AVEEVQPQI KDKSAWRKS TLNVANSTE 
      ETKDDSMRL NRLPVVEND RKTLISSLG ERSPTDKLT 
      LYIIKPRPR DSPPSLDDP PISNKVEID QDNIETPPA 
      TRKIFIPTP TEKREPTQP SPCRRLMGR PRDTDEPEP 
      NPTLGDGQF DRFSAARRT RRYKRTDTD PDEPKDEPE 
      TRLKAWQDK LQNPGRRHR HQTGINQDE VKLALTKTD 
      KKNEDEGFE ESQSLMSES PSQGASSGG NYEPEDSAL 
      VVYAKPLKS EPPVEKPAK GRGLERGGV RRTVQDTPK 
      KSVIPRRSG SLRKTNTST NVQRSSSRN SIVSSKSSL 
      NSSASACTV KKMPVKAGV GKAPPPKPV KRTPSTGGT 
      AGTRPPRPL AMSFMKPTT SSATKVHTV PKNGAFRTK
    CDS sequence
    • >TC011477 RA
      atggattcg aggatcggc ctggacttc atcgtggag 
      aatcccgag tacatcagg aaactggcg gcggccctc 
      gacaccagc aacgtcacc gtcaagaag caagtcttt 
      gagcttctg tccgcgttg tgtgtgcac aacgaagag 
      ggtcacgcg cgcgccctt gacactttg gagcactac 
      aagaaattg aaaggtgag agatataga ctgaatgtg 
      atcgtccag gagctgagc agggccacg gctgtggac 
      taccagacg gcccttgtg gcctttatc aactgtctg 
      atcatctcc acgcccagg ctcaccgac aggatcagg 
      agacgcaac gaattcatt ggatgtcat ctgcttccg 
      gtgctcaac aacttaaga aaatgcgcc gaggcggag 
      cccgagctc gccgtccag ctggacgtg ttcgacgag 
      cagcgcgac agcgacgac tcccagagc ctgcagggc 
      ccccacggc gtggacctc aactcgccc ttggacgtc 
      ttctacgcc atccttaaa caagttgcg gaaacgccg 
      caggaaatc cccttcctg agcatccta cagcacctg 
      ctgcgcatc gacgccaaa gacgccgtc agcgacatc 
      atctgggac acggccgag cgtctcgtg caccgcgcc 
      accctcctg gagaaccgc gaagacgcc gcccgcctg 
      ctgcgcgcc cccagcgcc caatcaaag ttcttctgc 
      cactgtcac ctccgctcc gagtctagt cccacccgc 
      cgcccctcc ctcaacacg ccccagagc cccacgccc 
      gcgctgccg ccgccgccg cccccgccc cccaacgtc 
      cccgccgtc ccgcccccc gtccccgcg ccgcccctc 
      aagcccgcc gcgccgctc ccgcccccc gaatgcgat 
      tctgtgata gagaagtta ccccagcag gagatcccg 
      gtgccgaag attaaaatg aagactttc aattggaat 
      aaaataccg aataataag gtgattggg aagaataat 
      atttggacg caagtggcg tacactcac cagcattcg 
      ccgatggct gatttggat tggctggag atggagggg 
      ctgttctgc caacaagcg cccccagcc aatgcggcg 
      gcgagtttc aggcgcgaa aatacgaat tcagagact 
      ttggatagg aggatacgc aaggaaaat tgcgagatt 
      acgcttctg gacgggaag aggagtctc aatgttaat 
      attttttta aaacagttt cggagctct aatgaggac 
      atcatccac ttgatccgg gaaggggaa catgatgat 
      atcggggcc gaaaaactc aaaggactc cttaaaata 
      ctaccggaa gttgatgaa ctagacatg ctcaagtcg 
      tttgatggc gatttcact aaattagga aacgccgaa 
      aaattttta atacaactg accaactta tccaattat 
      aaactacga atagaaagc atgcttctg aaagaagag 
      tttgcctca aatatgagc tacttggag cctagcatt 
      aaatcaatg attatggca gctcaagac ttgatgacg 
      aacaaaccg cttcaagaa gtcttgtac atgattctg 
      gtcgctggc aatttccta aatgcgggt ggctatgcc 
      gggaacgcg gccggtgtt aaattgagt tcgttgcaa 
      aaaatcaca gacattaga gcgaataaa ccaaatatg 
      aatttaata cattttgtg gcgttgcaa gctgagaag 
      aaaaacccc aaactgctg accttcacc gacaacata 
      agcgtgctg gaggacgca gccaagacg acagtggag 
      cagctccag aacgagatc aacgtgctc gacgtccgg 
      ataaaaaaa atcaagaaa caaatcgaa ctggcaacc 
      accgagccc gaaatcaaa gagcaaatg accgaattt 
      ttgcaaatg gccgaacgt gaagtctcc aacttgcaa 
      cagaacatg gaagagttg gagaaggtg cgtgtgcaa 
      ctggctgaa tttttctgc gaggacacg aattcgttc 
      aaacttgag gagtgtttc cggattttc cacgggttt 
      tactgcaag ttcaagcaa gcagttggc gataacgag 
      cgcaggagg atccaggag gagcaggcg aatgcgcgg 
      cgccggcaa cgcgaggag ttgctcgct tccaagagg 
      cggcaaatg gagaacttg gggggaacc cccgattcg 
      gacgtgccg ttcatcgac ttgcaaatc tacgacagc 
      aggcatggt tttccgatg aaaaaggga tggaaaacc 
      accaacggg acatccgaa gacgagctt tcctcagtc 
      accggctcc ccctctctc tcccggcgc cggctgggc 
      tcatttaac ggcgacccg gagaaatcc cccgacatc 
      acccctaat gggagcctg cggcggcgg cgcagccga 
      gtcttgtcg gaggaggac gaaggcaac ttgatggac 
      ttcctccgg gcttcggac aacaaccgc gagcgccgc 
      tcctggggg agtctggac cggtcatgg gcccgcaaa 
      gctagaggt gggagccag agaaaacgc ccggcttta 
      ttaggtgct gacttcacc tcggaccgc gaacgcccc 
      tcctcgccc tcacctcta agcgagtcc aaaccgatc 
      gtttcaacc cccgaagaa gaaaccaac aaaccgaaa 
      gaactgaga caaaaaatc gaatcctgg ctgcaggcc 
      agcgaaagc gaccagacc gaggacacc cgacggcag 
      actcgccgc atcacgaac cggcgctcc ttcgaaatg 
      gactcggag agtgaacgc agcagcact ctggacacg 
      ctgcccgaa ggcaagcaa gtctacagc ggcagccaa 
      tccacctac cgcaaggcc tacccggcc tgggagccc 
      tcgaagaca atcgacaac acggacgtg gtcagcgcc 
      atggaagcc gtcgaggaa gtgcagccc caaatcaag 
      gacaagtcg gcgtggcgc aaatccacc ctgaacgtg 
      gccaacagc acggaagaa accaaagac gactcaatg 
      cgtcttaac cgcctacca gtggtggag aacgaccgc 
      aaaaccctg attagctct ctcggggag cgctccccc 
      accgataaa ctcactctc tacattatc aagccacgc 
      ccccgggac tcgcccccc agcctggac gacccaccc 
      atcagcaac aaggtcgaa atcgaccaa gacaacata 
      gaaacgccc ccagccacg cgcaaaatc ttcatcccc 
      acgcccacc gagaagcgc gagccgacg cagcccagc 
      ccctgtcgc cgcctcatg ggccgccca cgcgacacg 
      gacgagccc gaacccaac ccgacgctc ggcgacggc 
      caattcgac cggttttcc gccgcaagg cgcacacga 
      cgctacaaa cgcaccgac accgacccc gacgaaccc 
      aaagacgag cccgagacc cgcctcaag gcgtggcaa 
      gacaagcta cagaacccc ggtaggcgc caccgccac 
      caaaccgga atcaaccaa gacgaagtc aagctagcc 
      ttgactaaa actgacaag aaaaacgaa gacgaaggc 
      ttcgaggag agccagagt ttaatgtcg gagtcgccg 
      agtcagggg gcgtcctct ggcggcaac tacgagccg 
      gaggactcc gccctagtc gtctacgcc aaaccgctg 
      aaaagcgag ccgccggtg gagaagccg gccaagggg 
      cggggcctg gagcggggc ggagtcagg cgcacggtg 
      caggacacg cccaaaaag agtgtgatt ccccgcagg 
      tcggggagt ctgcgcaag accaacacc tcgacgaat 
      gtgcaaagg tcgagttcg aggaatagt attgtcagt 
      tcgaaaagt tcgctcaat agttcggcg tccgcttgc 
      acggttaag aaaatgccg gttaaggcg ggtgttggc 
      aaagccccg cccccgaaa ccggtcaag agaacgccc 
      tcgactggg ggcacggcc gggacgaga cccccacga 
      cccctggcc atgtctttc atgaaacct acgacttct 
      agcgccact aaagtgcac acagtgccc aagaacggg 
      gcctttaga acaaagtaa
    • >TC011477 RB
      atgatagcg cataataga acctgtccg acccacaca 
      accaagtgg aagaggccg aagagctct gaagtgaaa 
      gtgtcggcc tctactccg tcgaggtcc gtccgagat 
      gcacagctg agtgcggcg ccactcttc aaatcgcca 
      ccacctcca aaccacacg acgtacgcg cgcccgaaa 
      cgcaagggg tgtttttta gcgtcggca gcgtcgcga 
      cgtccacga aaagtgccg ctggagccg acggttcgc 
      gcgctattt gtgaacgaa agtgcatgt gcaaaggga 
      ggaaaattg cacttttcg catgtttgc cggcttgga 
      tgtatccga aacaatcgg acgatggat tcgaggatc 
      ggcctggac ttcatcgtg gagaatccc gagtacatc 
      aggaaactg gcggcggcc ctcgacacc agcaacgtc 
      accgtcaag aagcaagtc tttgagctt ctgtccgcg 
      ttgtgtgtg cacaacgaa gagggtcac gcgcgcgcc 
      cttgacact ttggagcac tacaagaaa ttgaaaggt 
      gagagatat agactgaat gtgatcgtc caggagctg 
      agcagggcc acggctgtg gactaccag acggccctt 
      gtggccttt atcaactgt ctgatcatc tccacgccc 
      aggctcacc gacaggatc aggagacgc aacgaattc 
      attggatgt catctgctt ccggtgctc aacaactta 
      agaaaatgc gccgaggcg gagcccgag ctcgccgtc 
      cagctggac gtgttcgac gagcagcgc gacagcgac 
      gactcccag agcctgcag ggcccccac ggcgtggac 
      ctcaactcg cccttggac gtcttctac gccatcctt 
      aaacaagtt gcggaaacg ccgcaggaa atccccttc 
      ctgagcatc ctacagcac ctgctgcgc atcgacgcc 
      aaagacgcc gtcagcgac atcatctgg gacacggcc 
      gagcgtctc gtgcaccgc gccaccctc ctggagaac 
      cgcgaagac gccgcccgc ctgctgcgc gcccccagc 
      gcccaatca aagttcttc tgccactgt cacctccgc 
      tccgagtct agtcccacc cgccgcccc tccctcaac 
      acgccccag agccccacg cccgcgctg ccgccgccg 
      ccgcccccg ccccccaac gtccccgcc gtcccgccc 
      cccgtcccc gcgccgccc ctcaagccc gccgcgccg 
      ctcccgccc cccgaatgc gattctgtg atagagaag 
      ttaccccag caggagatc ccggtgccg aagattaaa 
      atgaagact ttcaattgg aataaaata ccgaataat 
      aaggtgatt gggaagaat aatatttgg acgcaagtg 
      gcgtacact caccagcat tcgccgatg gctgatttg 
      gattggctg gagatggag gggctgttc tgccaacaa 
      gcgccccca gccaatgcg gcggcgagt ttcaggcgc 
      gaaaatacg aattcagag actttggat aggaggata 
      cgcaaggaa aattgcgag attacgctt ctggacggg 
      aagaggagt ctcaatgtt aatattttt ttaaaacag 
      tttcggagc tctaatgag gacatcatc cacttgatc 
      cgggaaggg gaacatgat gatatcggg gccgaaaaa 
      ctcaaagga ctccttaaa atactaccg gaagttgat 
      gaactagac atgctcaag tcgtttgat ggcgatttc 
      actaaatta ggaaacgcc gaaaaattt ttaatacaa 
      ctgaccaac ttatccaat tataaacta cgaatagaa 
      agcatgctt ctgaaagaa gagtttgcc tcaaatatg 
      agctacttg gagcctagc attaaatca atgattatg 
      gcagctcaa gacttgatg acgaacaaa ccgcttcaa 
      gaagtcttg tacatgatt ctggtcgct ggcaatttc 
      ctaaatgcg ggtggctat gccgggaac gcggccggt 
      gttaaattg agttcgttg caaaaaatc acagacatt 
      agagcgaat aaaccaaat atgaattta atacatttt 
      gtggcgttg caagctgag aagaaaaac cccaaactg 
      ctgaccttc accgacaac ataagcgtg ctggaggac 
      gcagccaag acgacagtg gagcagctc cagaacgag 
      atcaacgtg ctcgacgtc cggataaaa aaaatcaag 
      aaacaaatc gaactggca accaccgag cccgaaatc 
      aaagagcaa atgaccgaa tttttgcaa atggccgaa 
      cgtgaagtc tccaacttg caacagaac atggaagag 
      ttggagaag gtgcgtgtg caactggct gaatttttc 
      tgcgaggac acgaattcg ttcaaactt gaggagtgt 
      ttccggatt ttccacggg ttttactgc aagttcaag 
      caagcagtt ggcgataac gagcgcagg aggatccag 
      gaggagcag gcgaatgcg cggcgccgg caacgcgag 
      gagttgctc gcttccaag aggcggcaa atggagaac 
      ttgggggga acccccgat tcggacgtg ccgttcatc 
      gacttgcaa atctacgac agcaggcat ggttttccg 
      atgaaaaag ggatggaaa accaccaac gggacatcc 
      gaagacgag ctttcctca gtcaccggc tccccctct 
      ctctcccgg cgccggctg ggctcattt aacggcgac 
      ccggagaaa tcccccgac atcacccct aatgggagc 
      ctgcggcgg cggcgcagc cgagtcttg tcggaggag 
      gacgaaggc aacttgatg gacttcctc cgggcttcg 
      gacaacaac cgcgagcgc cgctcctgg gggagtctg 
      gaccggtca tgggcccgc aaagctaga ggtgggagc 
      cagagaaaa cgcccggct ttattaggt gctgacttc 
      acctcggac cgcgaacgc ccctcctcg ccctcacct 
      ctaagcgag tccaaaccg atcgtttca acccccgaa 
      gaagaaacc aacaaaccg aaagaactg agacaaaaa 
      atcgaatcc tggctgcag gccagcgaa agcgaccag 
      accgaggac acccgacgg cagactcgc cgcatcacg 
      aaccggcgc tccttcgaa atggactcg gagagtgaa 
      cgcagcagc actctggac acgctgccc gaaggcaag 
      caagtctac agcggcagc caatccacc taccgcaag 
      gcctacccg gcctgggag ccctcgaag acaatcgac 
      aacacggac gtggtcagc gccatggaa gccgtcgag 
      gaagtgcag ccccaaatc aaggacaag tcggcgtgg 
      cgcaaatcc accctgaac gtggccaac agcacggaa 
      gaaaccaaa gacgactca atgcgtctt aaccgccta 
      ccagtggtg gagaacgac cgcaaaacc ctgattagc 
      tctctcggg gagcgctcc cccaccgat aaactcact 
      ctctacatt atcaagcca cgcccccgg gactcgccc 
      cccagcctg gacgaccca cccatcagc aacaaggtc 
      gaaatcgac caagacaac atagaaacg cccccagcc 
      acgcgcaaa atcttcatc cccacgccc accgagaag 
      cgcgagccg acgcagccc agcccctgt cgccgcctc 
      atgggccgc ccacgcgac acggacgag cccgaaccc 
      aacccgacg ctcggcgac ggccaattc gaccggttt 
      tccgccgca aggcgcaca cgacgctac aaacgcacc 
      gacaccgac cccgacgaa cccaaagac gagcccgag 
      acccgcctc aaggcgtgg caagacaag ctacagaac 
      cccggtagg cgccaccgc caccaaacc ggaatcaac 
      caagacgaa gtcaagcta gccttgact aaaactgac 
      aagaaaaac gaagacgaa ggcttcgag gagagccag 
      agtttaatg tcggagtcg ccgagtcag ggggcgtcc 
      tctggcggc aactacgag ccggaggac tccgcccta 
      gtcgtctac gccaaaccg ctgaaaagc gagccgccg 
      gtggagaag ccggccaag gggcggggc ctggagcgg 
      ggcggagtc aggcgcacg gtgcaggac acgcccaaa 
      aagagtgtg attccccgc aggtcgggg agtctgcgc 
      aagaccaac acctcgacg aatgtgcaa aggtcgagt 
      tcgaggaat agtattgtc agttcgaaa agttcgctc 
      aatagttcg gcgtccgct tgcacggtt aagaaaatg 
      ccggttaag gcgggtgtt ggcaaagcc ccgcccccg 
      aaaccggtc aagagaacg ccctcgact gggggcacg 
      gccgggacg agaccccca cgacccctg gccatgtct 
      ttcatgaaa cctacgact tctagcgcc actaaagtg 
      cacacagtg cccaagaac ggggccttt agaacaaag 
      taa
    mRNA sequence
    • >TC011477 RA
      tccgaccca cacaaccaa gtggaagag gccgaagag 
      ctctgaagt gaaagtgtc ggcctctac tccgtcgag 
      gtccgtccg agatgcaca gctgagtgc ggcgccact 
      cttcaaatc gccaccacc tccaaacca cacgacgta 
      cgcgcgccc gaaacgcaa ggggtgttt tttagcgtc 
      ggcagcgtc gcgacgtcc acgaaaagt gccgctgga 
      gccgacggt tcgcgcgct atttgtgaa cgaaagtgc 
      atgtgcaaa ggtgatgca aggattggg acattattc 
      acgtgctgc tggggattg tcgctgatg ccgggacga 
      cgataggga ggaaaattg cacttttcg catcaatcg 
      gacgatgga ttcgaggat cggcctgga cttcatcgt 
      ggagaatcc cgagtacat caggaaact ggcggcggc 
      cctcgacac cagcaacgt caccgtcaa gaagcaagt 
      ctttgagct tctgtccgc gttgtgtgt gcacaacga 
      agagggtca cgcgcgcgc ccttgacac tttggagca 
      ctacaagaa attgaaagg tgagagata tagactgaa 
      tgtgatcgt ccaggagct gagcagggc cacggctgt 
      ggactacca gacggccct tgtggcctt tatcaactg 
      tctgatcat ctccacgcc caggctcac cgacaggat 
      caggagacg caacgaatt cattggatg tcatctgct 
      tccggtgct caacaactt aagaaaatg cgccgaggc 
      ggagcccga gctcgccgt ccagctgga cgtgttcga 
      cgagcagcg cgacagcga cgactccca gagcctgca 
      gggccccca cggcgtgga cctcaactc gcccttgga 
      cgtcttcta cgccatcct taaacaagt tgcggaaac 
      gccgcagga aatcccctt cctgagcat cctacagca 
      cctgctgcg catcgacgc caaagacgc cgtcagcga 
      catcatctg ggacacggc cgagcgtct cgtgcaccg 
      cgccaccct cctggagaa ccgcgaaga cgccgcccg 
      cctgctgcg cgcccccag cgcccaatc aaagttctt 
      ctgccactg tcacctccg ctccgagtc tagtcccac 
      ccgccgccc ctccctcaa cacgcccca gagccccac 
      gcccgcgct gccgccgcc gccgccccc gccccccaa 
      cgtccccgc cgtcccgcc ccccgtccc cgcgccgcc 
      cctcaagcc cgccgcgcc gctcccgcc ccccgaatg 
      cgattctgt gatagagaa gttacccca gcaggagat 
      cccggtgcc gaagattaa aatgaagac tttcaattg 
      gaataaaat accgaataa taaggtgat tgggaagaa 
      taatatttg gacgcaagt ggcgtacac tcaccagca 
      ttcgccgat ggctgattt ggattggct ggagatgga 
      ggggctgtt ctgccaaca agcgccccc agccaatgc 
      ggcggcgag tttcaggcg cgaaaatac gaattcaga 
      gactttgga taggaggat acgcaagga aaattgcga 
      gattacgct tctggacgg gaagaggag tctcaatgt 
      taatatttt tttaaaaca gtttcggag ctctaatga 
      ggacatcat ccacttgat ccgggaagg ggaacatga 
      tgatatcgg ggccgaaaa actcaaagg actccttaa 
      aatactacc ggaagttga tgaactaga catgctcaa 
      gtcgtttga tggcgattt cactaaatt aggaaacgc 
      cgaaaaatt tttaataca actgaccaa cttatccaa 
      ttataaact acgaataga aagcatgct tctgaaaga 
      agagtttgc ctcaaatat gagctactt ggagcctag 
      cattaaatc aatgattat ggcagctca agacttgat 
      gacgaacaa accgcttca agaagtctt gtacatgat 
      tctggtcgc tggcaattt cctaaatgc gggtggcta 
      tgccgggaa cgcggccgg tgttaaatt gagttcgtt 
      gcaaaaaat cacagacat tagagcgaa taaaccaaa 
      tatgaattt aatacattt tgtggcgtt gcaagctga 
      gaagaaaaa ccccaaact gctgacctt caccgacaa 
      cataagcgt gctggagga cgcagccaa gacgacagt 
      ggagcagct ccagaacga gatcaacgt gctcgacgt 
      ccggataaa aaaaatcaa gaaacaaat cgaactggc 
      aaccaccga gcccgaaat caaagagca aatgaccga 
      atttttgca aatggccga acgtgaagt ctccaactt 
      gcaacagaa catggaaga gttggagaa ggtgcgtgt 
      gcaactggc tgaattttt ctgcgagga cacgaattc 
      gttcaaact tgaggagtg tttccggat tttccacgg 
      gttttactg caagttcaa gcaagcagt tggcgataa 
      cgagcgcag gaggatcca ggaggagca ggcgaatgc 
      gcggcgccg gcaacgcga ggagttgct cgcttccaa 
      gaggcggca aatggagaa cttgggggg aacccccga 
      ttcggacgt gccgttcat cgacttgca aatctacga 
      cagcaggca tggttttcc gatgaaaaa gggatggaa 
      aaccaccaa cgggacatc cgaagacga gctttcctc 
      agtcaccgg ctccccctc tctctcccg gcgccggct 
      gggctcatt taacggcga cccggagaa atcccccga 
      catcacccc taatgggag cctgcggcg gcggcgcag 
      ccgagtctt gtcggagga ggacgaagg caacttgat 
      ggacttcct ccgggcttc ggacaacaa ccgcgagcg 
      ccgctcctg ggggagtct ggaccggtc atgggcccg 
      caaagctag aggtgggag ccagagaaa acgcccggc 
      tttattagg tgctgactt cacctcgga ccgcgaacg 
      cccctcctc gccctcacc tctaagcga gtccaaacc 
      gatcgtttc aacccccga agaagaaac caacaaacc 
      gaaagaact gagacaaaa aatcgaatc ctggctgca 
      ggccagcga aagcgacca gaccgagga cacccgacg 
      gcagactcg ccgcatcac gaaccggcg ctccttcga 
      aatggactc ggagagtga acgcagcag cactctgga 
      cacgctgcc cgaaggcaa gcaagtcta cagcggcag 
      ccaatccac ctaccgcaa ggcctaccc ggcctggga 
      gccctcgaa gacaatcga caacacgga cgtggtcag 
      cgccatgga agccgtcga ggaagtgca gccccaaat 
      caaggacaa gtcggcgtg gcgcaaatc caccctgaa 
      cgtggccaa cagcacgga agaaaccaa agacgactc 
      aatgcgtct taaccgcct accagtggt ggagaacga 
      ccgcaaaac cctgattag ctctctcgg ggagcgctc 
      ccccaccga taaactcac tctctacat tatcaagcc 
      acgcccccg ggactcgcc ccccagcct ggacgaccc 
      acccatcag caacaaggt cgaaatcga ccaagacaa 
      catagaaac gcccccagc cacgcgcaa aatcttcat 
      ccccacgcc caccgagaa gcgcgagcc gacgcagcc 
      cagcccctg tcgccgcct catgggccg cccacgcga 
      cacggacga gcccgaacc caacccgac gctcggcga 
      cggccaatt cgaccggtt ttccgccgc aaggcgcac 
      acgacgcta caaacgcac cgacaccga ccccgacga 
      acccaaaga cgagcccga gacccgcct caaggcgtg 
      gcaagacaa gctacagaa ccccggtag gcgccaccg 
      ccaccaaac cggaatcaa ccaagacga agtcaagct 
      agccttgac taaaactga caagaaaaa cgaagacga 
      aggcttcga ggagagcca gagtttaat gtcggagtc 
      gccgagtca gggggcgtc ctctggcgg caactacga 
      gccggagga ctccgccct agtcgtcta cgccaaacc 
      gctgaaaag cgagccgcc ggtggagaa gccggccaa 
      ggggcgggg cctggagcg gggcggagt caggcgcac 
      ggtgcagga cacgcccaa aaagagtgt gattccccg 
      caggtcggg gagtctgcg caagaccaa cacctcgac 
      gaatgtgca aaggtcgag ttcgaggaa tagtattgt 
      cagttcgaa aagttcgct caatagttc ggcgtccgc 
      ttgcacggt taagaaaat gccggttaa ggcgggtgt 
      tggcaaagc cccgccccc gaaaccggt caagagaac 
      gccctcgac tgggggcac ggccgggac gagaccccc 
      acgacccct ggccatgtc tttcatgaa acctacgac 
      ttctagcgc cactaaagt gcacacagt gcccaagaa 
      cggggcctt tagaacaaa gtaatcact cattttttc 
      actagtcaa ggattatta ttacgaact aaatctttt 
      attcctac
    • >TC011477 RB
      ctgtaccag gagcggtct agttatccg ctaaccgtc 
      tatgatagc gcataatag aacctgtcc gacccacac 
      aaccaagtg gaagaggcc gaagagctc tgaagtgaa 
      agtgtcggc ctctactcc gtcgaggtc cgtccgaga 
      tgcacagct gagtgcggc gccactctt caaatcgcc 
      accacctcc aaaccacac gacgtacgc gcgcccgaa 
      acgcaaggg gtgtttttt agcgtcggc agcgtcgcg 
      acgtccacg aaaagtgcc gctggagcc gacggttcg 
      cgcgctatt tgtgaacga aagtgcatg tgcaaaggg 
      aggaaaatt gcacttttc gcatgtttg ccggcttgg 
      atgtatccg aaacaatcg gacgatgga ttcgaggat 
      cggcctgga cttcatcgt ggagaatcc cgagtacat 
      caggaaact ggcggcggc cctcgacac cagcaacgt 
      caccgtcaa gaagcaagt ctttgagct tctgtccgc 
      gttgtgtgt gcacaacga agagggtca cgcgcgcgc 
      ccttgacac tttggagca ctacaagaa attgaaagg 
      tgagagata tagactgaa tgtgatcgt ccaggagct 
      gagcagggc cacggctgt ggactacca gacggccct 
      tgtggcctt tatcaactg tctgatcat ctccacgcc 
      caggctcac cgacaggat caggagacg caacgaatt 
      cattggatg tcatctgct tccggtgct caacaactt 
      aagaaaatg cgccgaggc ggagcccga gctcgccgt 
      ccagctgga cgtgttcga cgagcagcg cgacagcga 
      cgactccca gagcctgca gggccccca cggcgtgga 
      cctcaactc gcccttgga cgtcttcta cgccatcct 
      taaacaagt tgcggaaac gccgcagga aatcccctt 
      cctgagcat cctacagca cctgctgcg catcgacgc 
      caaagacgc cgtcagcga catcatctg ggacacggc 
      cgagcgtct cgtgcaccg cgccaccct cctggagaa 
      ccgcgaaga cgccgcccg cctgctgcg cgcccccag 
      cgcccaatc aaagttctt ctgccactg tcacctccg 
      ctccgagtc tagtcccac ccgccgccc ctccctcaa 
      cacgcccca gagccccac gcccgcgct gccgccgcc 
      gccgccccc gccccccaa cgtccccgc cgtcccgcc 
      ccccgtccc cgcgccgcc cctcaagcc cgccgcgcc 
      gctcccgcc ccccgaatg cgattctgt gatagagaa 
      gttacccca gcaggagat cccggtgcc gaagattaa 
      aatgaagac tttcaattg gaataaaat accgaataa 
      taaggtgat tgggaagaa taatatttg gacgcaagt 
      ggcgtacac tcaccagca ttcgccgat ggctgattt 
      ggattggct ggagatgga ggggctgtt ctgccaaca 
      agcgccccc agccaatgc ggcggcgag tttcaggcg 
      cgaaaatac gaattcaga gactttgga taggaggat 
      acgcaagga aaattgcga gattacgct tctggacgg 
      gaagaggag tctcaatgt taatatttt tttaaaaca 
      gtttcggag ctctaatga ggacatcat ccacttgat 
      ccgggaagg ggaacatga tgatatcgg ggccgaaaa 
      actcaaagg actccttaa aatactacc ggaagttga 
      tgaactaga catgctcaa gtcgtttga tggcgattt 
      cactaaatt aggaaacgc cgaaaaatt tttaataca 
      actgaccaa cttatccaa ttataaact acgaataga 
      aagcatgct tctgaaaga agagtttgc ctcaaatat 
      gagctactt ggagcctag cattaaatc aatgattat 
      ggcagctca agacttgat gacgaacaa accgcttca 
      agaagtctt gtacatgat tctggtcgc tggcaattt 
      cctaaatgc gggtggcta tgccgggaa cgcggccgg 
      tgttaaatt gagttcgtt gcaaaaaat cacagacat 
      tagagcgaa taaaccaaa tatgaattt aatacattt 
      tgtggcgtt gcaagctga gaagaaaaa ccccaaact 
      gctgacctt caccgacaa cataagcgt gctggagga 
      cgcagccaa gacgacagt ggagcagct ccagaacga 
      gatcaacgt gctcgacgt ccggataaa aaaaatcaa 
      gaaacaaat cgaactggc aaccaccga gcccgaaat 
      caaagagca aatgaccga atttttgca aatggccga 
      acgtgaagt ctccaactt gcaacagaa catggaaga 
      gttggagaa ggtgcgtgt gcaactggc tgaattttt 
      ctgcgagga cacgaattc gttcaaact tgaggagtg 
      tttccggat tttccacgg gttttactg caagttcaa 
      gcaagcagt tggcgataa cgagcgcag gaggatcca 
      ggaggagca ggcgaatgc gcggcgccg gcaacgcga 
      ggagttgct cgcttccaa gaggcggca aatggagaa 
      cttgggggg aacccccga ttcggacgt gccgttcat 
      cgacttgca aatctacga cagcaggca tggttttcc 
      gatgaaaaa gggatggaa aaccaccaa cgggacatc 
      cgaagacga gctttcctc agtcaccgg ctccccctc 
      tctctcccg gcgccggct gggctcatt taacggcga 
      cccggagaa atcccccga catcacccc taatgggag 
      cctgcggcg gcggcgcag ccgagtctt gtcggagga 
      ggacgaagg caacttgat ggacttcct ccgggcttc 
      ggacaacaa ccgcgagcg ccgctcctg ggggagtct 
      ggaccggtc atgggcccg caaagctag aggtgggag 
      ccagagaaa acgcccggc tttattagg tgctgactt 
      cacctcgga ccgcgaacg cccctcctc gccctcacc 
      tctaagcga gtccaaacc gatcgtttc aacccccga 
      agaagaaac caacaaacc gaaagaact gagacaaaa 
      aatcgaatc ctggctgca ggccagcga aagcgacca 
      gaccgagga cacccgacg gcagactcg ccgcatcac 
      gaaccggcg ctccttcga aatggactc ggagagtga 
      acgcagcag cactctgga cacgctgcc cgaaggcaa 
      gcaagtcta cagcggcag ccaatccac ctaccgcaa 
      ggcctaccc ggcctggga gccctcgaa gacaatcga 
      caacacgga cgtggtcag cgccatgga agccgtcga 
      ggaagtgca gccccaaat caaggacaa gtcggcgtg 
      gcgcaaatc caccctgaa cgtggccaa cagcacgga 
      agaaaccaa agacgactc aatgcgtct taaccgcct 
      accagtggt ggagaacga ccgcaaaac cctgattag 
      ctctctcgg ggagcgctc ccccaccga taaactcac 
      tctctacat tatcaagcc acgcccccg ggactcgcc 
      ccccagcct ggacgaccc acccatcag caacaaggt 
      cgaaatcga ccaagacaa catagaaac gcccccagc 
      cacgcgcaa aatcttcat ccccacgcc caccgagaa 
      gcgcgagcc gacgcagcc cagcccctg tcgccgcct 
      catgggccg cccacgcga cacggacga gcccgaacc 
      caacccgac gctcggcga cggccaatt cgaccggtt 
      ttccgccgc aaggcgcac acgacgcta caaacgcac 
      cgacaccga ccccgacga acccaaaga cgagcccga 
      gacccgcct caaggcgtg gcaagacaa gctacagaa 
      ccccggtag gcgccaccg ccaccaaac cggaatcaa 
      ccaagacga agtcaagct agccttgac taaaactga 
      caagaaaaa cgaagacga aggcttcga ggagagcca 
      gagtttaat gtcggagtc gccgagtca gggggcgtc 
      ctctggcgg caactacga gccggagga ctccgccct 
      agtcgtcta cgccaaacc gctgaaaag cgagccgcc 
      ggtggagaa gccggccaa ggggcgggg cctggagcg 
      gggcggagt caggcgcac ggtgcagga cacgcccaa 
      aaagagtgt gattccccg caggtcggg gagtctgcg 
      caagaccaa cacctcgac gaatgtgca aaggtcgag 
      ttcgaggaa tagtattgt cagttcgaa aagttcgct 
      caatagttc ggcgtccgc ttgcacggt taagaaaat 
      gccggttaa ggcgggtgt tggcaaagc cccgccccc 
      gaaaccggt caagagaac gccctcgac tgggggcac 
      ggccgggac gagaccccc acgacccct ggccatgtc 
      tttcatgaa acctacgac ttctagcgc cactaaagt 
      gcacacagt gcccaagaa cggggcctt tagaacaaa 
      gtaatcact cattttttc actagtcaa ggattatta 
      ttacgaact aaatctttt attcctac
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