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Gene information
General information
TC030628 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 757886..759217
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030628
    iBeetle screen iB_02812
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030628 RA
      MARVPPKAP LSEQERLAL AAKLDQELD EFIDSLPKR 
      KPEEPVPFD RWEEDITNH PFFMKTVPE DGAPLHPLY 
      EGLQKLKYD PDENEPQDL AIAYKDDGN FNFKHKKYR 
      MAVISYTEG IKAKCGNDE IEATLYNNR SAAHFFLGN 
      HRSALADAE MALKLKPGY GKALVRAAN CCYKTGKFD 
      KAIEYCDQI LGGNDNNNN EEAQKLREM SLNSKKIAE 
      RDARRREKT ERKQQKEEA LIIGEILKR GIDIEGREN 
      LNLAKLEPQ FPQLFNSRV AIRDGRLVW PVVLFYPEY 
      KIMDYIQEF DECDCFSEQ LSQVFETRP EWDCEGKYR 
      VGNLNVYFE DKKNRIVPV DVSRTLLEV LKLEGHVIR 
      EGTPKFIIL PANSRVEKL FLARSL
    CDS sequence
    • >TC030628 RA
      atggcacgt gtgccccct aaagccccc ctttcggag 
      caagagcgc ctggccttg gccgcgaag ctcgaccag 
      gagctggac gagttcatc gacagcctc ccgaaacgc 
      aagccggag gagcccgtc ccgttcgac agatgggag 
      gaagacatc acaaatcac ccctttttc atgaaaacg 
      gtgcccgag gacggggca ccgctgcac cccctttac 
      gaaggcctc caaaagctg aagtacgac ccggacgaa 
      aatgagccc caagacctg gcaattgca tacaaagac 
      gacgggaat ttcaatttc aagcacaaa aagtaccgc 
      atggctgtt atttcatac acggagggg atcaaagca 
      aagtgcggc aatgacgaa atcgaggcc acactgtac 
      aataaccgg tctgcggct catttcttc ctggggaat 
      caccggtcg gcgcttgcc gacgccgaa atggcgcta 
      aaattgaag cctggctac gggaaagcg ctagtcagg 
      gctgccaat tgttgctac aagactggc aaatttgac 
      aaggctatt gagtattgt gaccaaatt ttagggggg 
      aatgataat aataataac gaagaggcg caaaagttg 
      cgcgaaatg tcgctaaac tcgaagaaa atcgccgaa 
      cgcgacgcc cgtcgcagg gaaaaaacc gagcgaaag 
      caacaaaaa gaggaggcg ttgataatc ggggaaata 
      ctcaagcgg gggattgac atcgaaggg cgcgaaaat 
      ttgaatttg gccaagctt gagccgcaa tttccgcag 
      cttttcaac agtcgggtc gcgattaga gatggacgg 
      ttggtttgg ccggtggtt ttattttac cccgagtat 
      aaaataatg gattacatt caagaattt gacgagtgt 
      gattgtttt tccgagcaa ttgtcgcaa gtgttcgag 
      accaggccc gagtgggat tgcgagggg aagtaccgg 
      gttgggaac ttaaacgtt tattttgag gacaaaaaa 
      aatcggatt gtcccagtt gacgtcagt cgaacgtta 
      ttagaagtt ttaaaatta gaagggcat gtaatacgt 
      gaagggacc cccaagttt atcatcctt ccggcgaat 
      agtcgagtc gagaaacta ttcttggct cggtcttta 
      taa
    mRNA sequence
    • >TC030628 RA
      aggttatgg cacgtgtgc cccctaaag ccccccttt 
      cggagcaag agcgcctgg ccttggccg cgaagctcg 
      accaggagc tggacgagt tcatcgaca gcctcccga 
      aacgcaagc cggaggagc ccgtcccgt tcgacagat 
      gggaggaag acatcacaa atcacccct ttttcatga 
      aaacggtgc ccgaggacg gggcaccgc tgcaccccc 
      tttacgaag gcctccaaa agctgaagt acgacccgg 
      acgaaaatg agccccaag acctggcaa ttgcataca 
      aagacgacg ggaatttca atttcaagc acaaaaagt 
      accgcatgg ctgttattt catacacgg aggggatca 
      aagcaaagt gcggcaatg acgaaatcg aggccacac 
      tgtacaata accggtctg cggctcatt tcttcctgg 
      ggaatcacc ggtcggcgc ttgccgacg ccgaaatgg 
      cgctaaaat tgaagcctg gctacggga aagcgctag 
      tcagggctg ccaattgtt gctacaaga ctggcaaat 
      ttgacaagg ctattgagt attgtgacc aaattttag 
      gggggaatg ataataata ataacgaag aggcgcaaa 
      agttgcgcg aaatgtcgc taaactcga agaaaatcg 
      ccgaacgcg acgcccgtc gcagggaaa aaaccgagc 
      gaaagcaac aaaaagagg aggcgttga taatcgggg 
      aaatactca agcggggga ttgacatcg aagggcgcg 
      aaaatttga atttggcca agcttgagc cgcaatttc 
      cgcagcttt tcaacagtc gggtcgcga ttagagatg 
      gacggttgg tttggccgg tggttttat tttaccccg 
      agtataaaa taatggatt acattcaag aatttgacg 
      agtgtgatt gtttttccg agcaattgt cgcaagtgt 
      tcgagacca ggcccgagt gggattgcg aggggaagt 
      accgggttg ggaacttaa acgtttatt ttgaggaca 
      aaaaaaatc ggattgtcc cagttgacg tcagtcgaa 
      cgttattag aagttttaa aattagaag ggcatgtaa 
      tacgtgaag ggaccccca agtttatca tccttccgg 
      cgaatagtc gagtcgaga aactattct tggctcggt 
      ctttataaa attcataca gttatttgt gtaagtaaa 
      agtccggga atagatttg tgaataatt aataaagta 
      ttgattcag tt