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Gene information
General information
TC033321
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015307
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 666222..668509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033321
    iBeetle screen iB_02811
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033321 RA
      MATNDETEN DNKMFGPLV FKPSGPPET ETEATCLLC 
      DNTFNITLR FDIFVRHLF EVHYLVIEE VQNIANLPQ 
      YCQYWRDKF KETPIEEII PSIKIDSSN QTYFLLSTL 
      LKEDKLLRH KLHLEHVLR VQEFERVDG GYQRPCLFC 
      KLHFEGTRT DFIRHLESQ HNLQLGNPQ NLVFVEELL 
      EKIDEHLAK LTCIFCQRV FPERSVLKE HMRKKLHKR 
      INPRNADYD KYFIANYLE PDKLGKKAQ ERDVMLQQD 
      DSNNDEEYD DWYEKDDFI TCLFCEKRD TNINTLCLH 
      MEADHDFDF AELTQNFDF YQKVKLVNY VRRQVHNHK 
      CFYCDLSCK SAKNLQEHL FEAQHCKIP DLKIFDQPE 
      FYFPTYEND ALLYLIDDV ED
    CDS sequence
    • >TC033321 RA
      atggcaacc aacgacgag acagaaaat gacaataaa 
      atgtttggc ccccttgtg ttcaagccg tcgggcccc 
      ccggagacc gaaaccgaa gcaacttgt ctcctgtgc 
      gataacacg tttaacata actttacgt ttcgatatt 
      ttcgtacgc catttattc gaagtgcac tatttggtg 
      attgaagaa gtgcaaaat attgcaaat ttgccacaa 
      tattgtcag tactggcgg gacaaattc aaggaaacc 
      ccaatcgag gaaataata ccatctata aaaatcgat 
      tcctccaat caaacctac ttcctgtta tcaacccta 
      ctgaaagaa gacaaactt ttgcgccac aaactccac 
      ctcgagcac gttttgcgc gtgcaagag ttcgagcgc 
      gtggatggg ggctatcaa agaccttgt cttttctgt 
      aaattgcac ttcgaaggc acacgaacc gatttcatc 
      cgccactta gaatcgcaa cacaatctc caactgggg 
      aatccccaa aacttggtt ttcgtcgaa gaactgttg 
      gaaaaaatc gacgagcat ttggcgaaa ctgacttgc 
      attttctgc cagcgggtt ttccccgaa cgtagcgtc 
      ctgaaggag cacatgcgc aaaaaactc cacaaacgg 
      attaacccg cgcaacgcc gactatgat aaatacttc 
      atcgcgaat tatctggag cctgacaag ttggggaaa 
      aaggcgcaa gaacgtgac gtcatgttg cagcaagat 
      gattcgaac aatgatgag gagtatgac gattggtac 
      gaaaaggac gattttatt acgtgtctc ttctgcgag 
      aaacgggac acgaatatc aacactttg tgcctccat 
      atggaggca gatcacgat tttgatttt gcggaattg 
      acgcaaaat ttcgatttc taccaaaag gttaagtta 
      gtgaactat gtgaggcgg caagtgcac aaccacaag 
      tgtttttat tgcgatttg agctgcaaa agtgcgaaa 
      aatttgcaa gagcattta tttgaagcc caacattgc 
      aaaatcccc gatttgaaa attttcgat caacctgaa 
      ttttacttc cccacgtac gagaatgac gcccttctc 
      tatttaatt gatgatgtt gaagattaa
    mRNA sequence
    • >TC033321 RA
      tgtcactgt cattgtcac ctttgacag tcttttttt 
      ctctattag aaagtttca aaattttgg ctgtttttg 
      gcactagat tgggccctt tttgccaat ggcaaccaa 
      cgacgagac agaaaatga caataaaat gtttggccc 
      ccttgtgtt caagccgtc gggcccccc ggagaccga 
      aaccgaagc aacttgtct cctgtgcga taacacgtt 
      taacataac tttacgttt cgatatttt cgtacgcca 
      tttattcga agtgcacta tttggtgat tgaagaagt 
      gcaaaatat tgcaaattt gccacaata ttgtcagta 
      ctggcggga caaattcaa ggaaacccc aatcgagga 
      aataatacc atctataaa aatcgattc ctccaatca 
      aacctactt cctgttatc aaccctact gaaagaaga 
      caaactttt gcgccacaa actccacct cgagcacgt 
      tttgcgcgt gcaagagtt cgagcgcgt ggatggggg 
      ctatcaaag accttgtct tttctgtaa attgcactt 
      cgaaggcac acgaaccga tttcatccg ccacttaga 
      atcgcaaca caatctcca actggggaa tccccaaaa 
      cttggtttt cgtcgaaga actgttgga aaaaatcga 
      cgagcattt ggcgaaact gacttgcat tttctgcca 
      gcgggtttt ccccgaacg tagcgtcct gaaggagca 
      catgcgcaa aaaactcca caaacggat taacccgcg 
      caacgccga ctatgataa atacttcat cgcgaatta 
      tctggagcc tgacaagtt ggggaaaaa ggcgcaaga 
      acgtgacgt catgttgca gcaagatga ttcgaacaa 
      tgatgagga gtatgacga ttggtacga aaaggacga 
      ttttattac gtgtctctt ctgcgagaa acgggacac 
      gaatatcaa cactttgtg cctccatat ggaggcaga 
      tcacgattt tgattttgc ggaattgac gcaaaattt 
      cgatttcta ccaaaaggt taagttagt gaactatgt 
      gaggcggca agtgcacaa ccacaagtg tttttattg 
      cgatttgag ctgcaaaag tgcgaaaaa tttgcaaga 
      gcatttatt tgaagccca acattgcaa aatccccga 
      tttgaaaat tttcgatca acctgaatt ttacttccc 
      cacgtacga gaatgacgc ccttctcta tttaattga 
      tgatgttga agattaaat aaattgaaa ttcgaattt 
      ggc