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Gene information
General information
TC015359 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid39: 35051..36964
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015359
    iBeetle screen iB_02810
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015359 RA
      MAKEEEDDK LPDQDAAKE FYAKYEPKE ILGRGISST 
      VRRCIEKET GKAFAAKII DLSNDNNSE GVSAKEATK 
      QEVAILRHV AGHSYIIEL QDVFESPTF IFLVFELCK 
      NGELFDYLT SVVTLSEKK TRYIMRQVL EGVAHIHAR 
      NIVHRDLKP ENILLDDNL NVKITDFGF AKKLAEGEL 
      VHELCGTPG YLAPETLKC SMFEDAPGY SYEVDVWAC 
      GVIMYTLLV GCPPFWHRK QMVMLRNIM EGKYSFSSP 
      EWADISEEP KDLIRRLLV VDPKQRISI DDALRHPFF 
      HTMQLWDQD IAPLKYALR KNSRRFSRI SEMALKLHH 
      RKFQARQKF QWAILVVRA MIRLQRLKF TPEPLSLDT 
      ARTDPYRVK LLRKIVDGC AFRVYGHWV KKGEGQNRA 
      ALFENIPKT ELKHIYVTN LSR
    CDS sequence
    • >TC015359 RA
      atggctaaa gaagaagaa gacgataaa ttaccagat 
      caagatgct gcaaaagag ttttatgca aaatatgaa 
      ccaaaggaa attcttgga agaggaata agttcgacc 
      gttcgccga tgtattgaa aaagaaacg ggtaaagcg 
      tttgcggca aaaataatc gatttaagc aatgataac 
      aacagtgaa ggtgtctca gctaaagag gcaacgaag 
      caagaagtt gccattttg cgtcacgtg gccggccat 
      tcgtacatt attgaatta caagacgtt tttgaatca 
      cccacgttc atatttttg gtttttgaa ttgtgcaaa 
      aacggtgaa ttgtttgat tatctcacc tcagtggtg 
      actttgtca gagaaaaaa acaaggtat ataatgcgg 
      caggtgttg gaaggagtg gcgcatata cacgctaga 
      aatatagtc catagagac cttaaaccg gaaaatatc 
      cttcttgac gataatctc aacgtaaaa attaccgat 
      tttggtttt gcaaaaaaa ttagcagag ggagaattg 
      gttcatgaa ttgtgtgga actccgggt tatttagca 
      ccggaaact ttaaagtgt agcatgttt gaagacgct 
      cccggctat tcgtatgaa gttgacgtg tgggcttgt 
      ggtgtcatt atgtacaca cttttagta ggttgtccc 
      ccgttttgg caccgcaaa caaatggtc atgttgcgt 
      aacataatg gaggggaag tactcgttt tcctcgccc 
      gaatgggcc gatatttca gaggaacca aaggatttg 
      attcgacgt ttactagta gtcgacccg aaacaacgc 
      atttcaatt gatgacgcg ttaaggcat ccgttcttc 
      catacgatg caattgtgg gatcaggat atcgcacca 
      ttgaaatat gcattgcga aagaacagt cgcagattc 
      agtagaatt tcagagatg gctttgaaa ttacatcat 
      cgtaagttt caagcccga caaaaattc caatgggcc 
      attttggtg gttagggca atgatacgt ttacaacga 
      ttgaagttt acaccggaa cctttgagt ttggatacg 
      gctaggacc gatccatat cgtgttaaa cttttgaga 
      aaaattgtt gatggttgt gcgtttcga gtttatgga 
      cattgggtg aaaaaaggc gaagggcaa aacagagcc 
      gcccttttt gaaaatata ccaaaaacc gaattgaaa 
      catatctac gtcaccaat ttaagccga taa
    mRNA sequence
    • >TC015359 RA
      gttattttt agttttaat ttggataaa atcatggct 
      aaagaagaa gaagacgat aaattacca gatcaagat 
      gctgcaaaa gagttttat gcaaaatat gaaccaaag 
      gaaattctt ggaagagga ataagttcg accgttcgc 
      cgatgtatt gaaaaagaa acgggtaaa gcgtttgcg 
      gcaaaaata atcgattta agcaatgat aacaacagt 
      gaaggtgtc tcagctaaa gaggcaacg aagcaagaa 
      gttgccatt ttgcgtcac gtggccggc cattcgtac 
      attattgaa ttacaagac gtttttgaa tcacccacg 
      ttcatattt ttggttttt gaattgtgc aaaaacggt 
      gaattgttt gattatctc acctcagtg gtgactttg 
      tcagagaaa aaaacaagg tatataatg cggcaggtg 
      ttggaagga gtggcgcat atacacgct agaaatata 
      gtccataga gaccttaaa ccggaaaat atccttctt 
      gacgataat ctcaacgta aaaattacc gattttggt 
      tttgcaaaa aaattagca gagggagaa ttggttcat 
      gaattgtgt ggaactccg ggttattta gcaccggaa 
      actttaaag tgtagcatg tttgaagac gctcccggc 
      tattcgtat gaagttgac gtgtgggct tgtggtgtc 
      attatgtac acactttta gtaggttgt cccccgttt 
      tggcaccgc aaacaaatg gtcatgttg cgtaacata 
      atggagggg aagtactcg ttttcctcg cccgaatgg 
      gccgatatt tcagaggaa ccaaaggat ttgattcga 
      cgtttacta gtagtcgac ccgaaacaa cgcatttca 
      attgatgac gcgttaagg catccgttc ttccatacg 
      atgcaattg tgggatcag gatatcgca ccattgaaa 
      tatgcattg cgaaagaac agtcgcaga ttcagtaga 
      atttcagag atggctttg aaattacat catcgtaag 
      tttcaagcc cgacaaaaa ttccaatgg gccattttg 
      gtggttagg gcaatgata cgtttacaa cgattgaag 
      tttacaccg gaacctttg agtttggat acggctagg 
      accgatcca tatcgtgtt aaacttttg agaaaaatt 
      gttgatggt tgtgcgttt cgagtttat ggacattgg 
      gtgaaaaaa ggcgaaggg caaaacaga gccgccctt 
      tttgaaaat ataccaaaa accgaattg aaacatatc 
      tacgtcacc aatttaagc cgataacaa atcggcttc 
      tgtttagtt tcaatttag actaattaa taactcgtt 
      tgtgataat ttttaattt acgtcctta ggaaatgtg 
      tttggtttc ttgctgttt ttatgtaca tcataaatc 
      tgtattatc aataaatta gagaaaaga ac