Status
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Gene information
General information
TC030698 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15268393..15272624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030698
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02809

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09134

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030698 RA
      MLAPEAGST NNPDVADKT PLVGHSRGF MKKIAGLLG 
      QNEGPSSDS GPSYVEFGS FTEQSETRT LGTFAGVFS 
      PVTLSMFSA LIFLRMGYI VGNAGLLVT LAQFMIAYL 
      ILVFTVSSI CAISTNGAV EGGGAYFMI SRTLGPEFG 
      GSIGTLFFM ANIVSSALC IAGCVEGLV ENFGPSGYL 
      VKGLIPDGH WWQFLYCVS LNTLNLLIC LIGAAMFAK 
      TTVIILTIV SICLGITIF SFLYQGPKQ VAVPDSNSL 
      IFQNETIRV FENYTGLYT ETLRSNLWQ QYGKDYTAN 
      GELVNFASV FGVLFSGVT GIMAGANMS GELKNPGKS 
      IPSGTLSAV GFTLIVYIM VSFFTAFTC TADLMQNNY 
      LFMAGVSVF PPSVAIGLL TATWSAALS NVIGGSRVL 
      EALAKDNVF GPLLSFIPK GTWKGNPVA AVLVSWLLV 
      QLVLFIGSL NIIAQLNSV LFLLSYLAT NLACLGLEL 
      ASAPNFRPS FKYFTWHTA FIGLLGTII MMFVISPIY 
      AACSILLCL CLVVFLHLF SPSKEAHWG SISQALIFH 
      QVRKYLLLL DSRKDHVKF WRPQMLLLV NSPRSACPL 
      IDFINDLKK GGLYVLGHV VKGDPDLPG PDPTLDMQL 
      HWLNLVDHL KVKAFVELT VAPSVRDGL RHLARLSGM 
      GAMKPNTVV LGFLDEEHQ QDFLQSSDS SYQNNDFGN 
      DVFPLNSQE KLEALEYVQ MMGDVLKMN KNLCLCRNF 
      SRLKKDTKK NWSKKYIDV WPVNFLNPG EMDAFDTTS 
      LFMMQLACI VNMVPLWSR LKLRLCICD EARQTRFTL 
      SPNGQSHME KLNLLLKRL RIAAVPFPV PGWSNVIES 
      HGNHMETYL KSVNNLILQ QTSDTVVTF IYLPPPPND 
      DSEAEAYYQ KLDIISRDL PPTMFVHGV STVTSTTL
    CDS sequence
    • >TC030698 RA
      atgttagcc cctgaggca ggctcaacg aacaacccc 
      gatgtggcc gataagacc cctttagtg ggccacagt 
      cgcggcttt atgaaaaaa atcgctggt cttttgggc 
      caaaatgag gggccgtcg agtgactca ggccctagt 
      tatgtcgag tttgggtct ttcactgaa caatcagag 
      accaggact ttgggaacg tttgcggga gttttcagt 
      cccgtgacc ttatctatg ttttcagca ctgattttt 
      ctgcgaatg gggtatatc gtcggaaat gcgggatta 
      ttggtcact ctagcacag tttatgata gcttatctc 
      attctggtg tttacggtc tcgtcgatt tgtgcgatt 
      tcgacgaac ggggctgtg gaagggggc ggtgcctat 
      tttatgata agccggacc ctagggccc gaattcggg 
      ggctcaatc ggcacgtta ttttttatg gcaaatatc 
      gtcagcagt gccctatgt atcgcaggg tgcgtggaa 
      ggcctagtc gaaaatttt ggcccttcc ggttattta 
      gtcaaggga ttaatcccg gacggccac tggtggcag 
      ttcctctac tgcgtcagt ctcaatact ttaaacctt 
      ctaatttgc ttaataggg gctgccatg tttgccaaa 
      accacagtt ataattctc acaatcgtg tcaatatgt 
      ctaggaatc acaattttc agtttcttg taccaaggc 
      cccaaacaa gtggccgtg cctgactca aacagtctc 
      atttttcaa aatgagaca atccgtgtt tttgaaaat 
      tacacaggg ttgtatacg gaaacgttg cggtcgaat 
      ttgtggcaa cagtacggc aaggattac acagctaat 
      ggtgaattg gtgaatttt gcctcagtt tttggggtg 
      cttttttcg ggggttact ggaatcatg gcaggggcc 
      aacatgagc ggtgaatta aaaaatccg ggaaagtcg 
      atcccgagt ggcacttta tcggccgta ggcttcact 
      ttaatagtg tacataatg gtttcattt tttaccgct 
      tttacttgt acggccgat ttgatgcaa aacaactac 
      ttatttatg gcgggggtt agtgttttt ccccctagt 
      gtggcaata gggctgtta acagccacg tggtcggcg 
      gctttaagc aacgtaatt gggggctcc cgtgtcttg 
      gaggcttta gcaaaagat aatgttttt ggtccgcta 
      ttatccttc atcccgaaa gggacatgg aagggcaac 
      cccgtggcc gccgttcta gtctcctgg ctccttgtc 
      caacttgtc ctttttatc ggatccctc aatatcatc 
      gcccagtta aattcagtc ctctttctt ttaagctac 
      ttagccacg aatttagct tgtttgggc cttgaactc 
      gcaagcgcc cctaatttc cgtccctct ttcaaatat 
      ttcacctgg catactgcc ttcatcggg cttttgggg 
      actataatc atgatgttt gtgattagt ccaatttac 
      gccgcctgt agcattctc ttgtgtctc tgtttggtt 
      gtatttctg cacctgttt tcgccctcg aaagaggcg 
      cattgggga agtatctcc caagccttg atcttccac 
      caagtccgc aaatacctc ctcctgctt gactcccgc 
      aaagaccat gtgaaattc tggcggccc caaatgctc 
      ctacttgtg aatagccct cgttcggcg tgtccccta 
      atcgatttt atcaacgat ttgaaaaaa ggagggttg 
      tacgtcctg gggcatgtc gtaaaaggc gaccctgat 
      ttgccaggg ccggaccca actctcgac atgcagttg 
      cattggtta aatttggtc gatcatttg aaagtcaag 
      gcttttgtc gagttgact gtggccccc tctgtgagg 
      gacggcttg agacatttg gcaagactg tcaggaatg 
      ggggccatg aagcctaat actgtggtt ttgggtttt 
      ttggatgag gaacaccaa caagatttt ctgcaaagt 
      agcgactcc tcgtaccaa aataacgat tttggtaat 
      gatgtgttt cctctgaat tcgcaagaa aagctggag 
      gctttggag tacgtccaa atgatgggg gacgtactc 
      aagatgaac aaaaaccta tgtttgtgt cggaatttt 
      tcgcggttg aagaaagac acgaagaaa aattggtca 
      aagaaatat atagatgtg tggcctgtt aatttctta 
      aatccgggc gagatggat gcgtttgat acgacttcg 
      ttgtttatg atgcagttg gcgtgtatt gttaacatg 
      gtgcccctt tggagtcgc cttaaactg cgtctgtgt 
      atttgtgac gaggcgaga cagactcgc tttacatta 
      agtccgaac gggcaaagt cacatggag aagttaaat 
      ttgttgttg aagagattg aggattgcg gcggtgccg 
      tttccagtt cccgggtgg agtaatgtg attgaatcg 
      cacggaaat cacatggaa acatactta aaaagcgtt 
      aataatttg atattgcaa caaacttca gacacggta 
      gtgaccttt atatacctt ccaccgccc ccaaatgac 
      gattctgag gcagaggcc tattaccaa aaattggac 
      attatctca cgggacctt cccccgacg atgtttgtt 
      cacggcgtc agcacagtt acgtccaca actttataa
    mRNA sequence
    • >TC030698 RA
      ctttcaatg aaatggtgg acaaattgg aaaagaaac 
      tccagtcga aaaatttac ccgtttatt gcaggaatc 
      gggaattat tgatagagt gtctggggc gcaacccca 
      cctgtatgt taaattgca aaaaagaaa tggatgagt 
      tcattgact gtgttgacg cgctgcgag tgcgacgca 
      tcaaagaac gcatccaag actccatgc acaagaaaa 
      aaatcgact tggacgcac tcgtcaaaa gctaattat 
      cgtttttct aaactcttg aacttggcc tctaaccgt 
      tttgagtac gcgtccaat ttatatgca gcctgttct 
      aatttcaca acactgtcc tcaatttga tcaatttgt 
      tgcaagtac ggttgcaga gaattgtct aaaaattgt 
      caataaatc gtaatatat aatgcgaaa ttcaggtta 
      catttctcg tttaattct tgcatgctt tttgaaacg 
      ttagcagcg atgtgtttc atgtctgaa tatttcgta 
      atcgtcgca cgattcatg ttttcaagc aaacggtaa 
      tcatcgaga gttgaagtt aactcgccg tataagtaa 
      tcagacgtt ttattaaac atggtagtg gctaaacgg 
      ctcaaattg gggtcgtgc gggtccaaa gcctcgaaa 
      ctcgaagtc gaggtcgac aaagtcgga cctaacgaa 
      atcattact tcgggaaga tgtgacgcg cgtttacct 
      tttttgggg gagtctgtc acaccctcg gagtcaact 
      tcttcagcc atcgtaatt tttttccaa atttgttaa 
      taaaaaact acataacct caagtgtca taactcaaa 
      gtcggcaat acagaagcc tgtaacttt cgattacat 
      tttttacca aattatcaa ttttgtttg gttgatttc 
      caatcgtag ctaattcta gcgatgagc ttatcaaat 
      gccaataaa aaaaaactt tcaaacaaa cactaaaat 
      cagcatttt tccgcgtta tcattttat aggggtcgc 
      ccccagaaa cgtcaaatt gacagttat ttgtttact 
      tgtcatgag tttgtgtgt gggtgttta gtggcgaaa 
      tgttagccc ctgaggcag gctcaacga acaaccccg 
      atgtggccg ataagaccc ctttagtgg gccacagtc 
      gcggcttta tgaaaaaaa tcgctggtc ttttgggcc 
      aaaatgagg ggccgtcga gtgactcag gccctagtt 
      atgtcgagt ttgggtctt tcactgaac aatcagaga 
      ccaggactt tgggaacgt ttgcgggag ttttcagtc 
      ccgtgacct tatctatgt tttcagcac tgatttttc 
      tgcgaatgg ggtatatcg tcggaaatg cgggattat 
      tggtcactc tagcacagt ttatgatag cttatctca 
      ttctggtgt ttacggtct cgtcgattt gtgcgattt 
      cgacgaacg gggctgtgg aagggggcg gtgcctatt 
      ttatgataa gccggaccc tagggcccg aattcgggg 
      gctcaatcg gcacgttat tttttatgg caaatatcg 
      tcagcagtg ccctatgta tcgcagggt gcgtggaag 
      gcctagtcg aaaattttg gcccttccg gttatttag 
      tcaagggat taatcccgg acggccact ggtggcagt 
      tcctctact gcgtcagtc tcaatactt taaaccttc 
      taatttgct taatagggg ctgccatgt ttgccaaaa 
      ccacagtta taattctca caatcgtgt caatatgtc 
      taggaatca caattttca gtttcttgt accaaggcc 
      ccaaacaag tggccgtgc ctgactcaa acagtctca 
      tttttcaaa atgagacaa tccgtgttt ttgaaaatt 
      acacagggt tgtatacgg aaacgttgc ggtcgaatt 
      tgtggcaac agtacggca aggattaca cagctaatg 
      gtgaattgg tgaattttg cctcagttt ttggggtgc 
      ttttttcgg gggttactg gaatcatgg caggggcca 
      acatgagcg gtgaattaa aaaatccgg gaaagtcga 
      tcccgagtg gcactttat cggccgtag gcttcactt 
      taatagtgt acataatgg tttcatttt ttaccgctt 
      ttacttgta cggccgatt tgatgcaaa acaactact 
      tatttatgg cgggggtta gtgtttttc cccctagtg 
      tggcaatag ggctgttaa cagccacgt ggtcggcgg 
      ctttaagca acgtaattg ggggctccc gtgtcttgg 
      aggctttag caaaagata atgtttttg gtccgctat 
      tatccttca tcccgaaag ggacatgga agggcaacc 
      ccgtggccg ccgttctag tctcctggc tccttgtcc 
      aacttgtcc tttttatcg gatccctca atatcatcg 
      cccagttaa attcagtcc tctttcttt taagctact 
      tagccacga atttagctt gtttgggcc ttgaactcg 
      caagcgccc ctaatttcc gtccctctt tcaaatatt 
      tcacctggc atactgcct tcatcgggc ttttgggga 
      ctataatca tgatgtttg tgattagtc caatttacg 
      ccgcctgta gcattctct tgtgtctct gtttggttg 
      tatttctgc acctgtttt cgccctcga aagaggcgc 
      attggggaa gtatctccc aagccttga tcttccacc 
      aagtccgca aatacctcc tcctgcttg actcccgca 
      aagaccatg tgaaattct ggcggcccc aaatgctcc 
      tacttgtga atagccctc gttcggcgt gtcccctaa 
      tcgatttta tcaacgatt tgaaaaaag gagggttgt 
      acgtcctgg ggcatgtcg taaaaggcg accctgatt 
      tgccagggc cggacccaa ctctcgaca tgcagttgc 
      attggttaa atttggtcg atcatttga aagtcaagg 
      cttttgtcg agttgactg tggccccct ctgtgaggg 
      acggcttga gacatttgg caagactgt caggaatgg 
      gggccatga agcctaata ctgtggttt tgggttttt 
      tggatgagg aacaccaac aagattttc tgcaaagta 
      gcgactcct cgtaccaaa ataacgatt ttggtaatg 
      atgtgtttc ctctgaatt cgcaagaaa agctggagg 
      ctttggagt acgtccaaa tgatggggg acgtactca 
      agatgaaca aaaacctat gtttgtgtc ggaattttt 
      cgcggttga agaaagaca cgaagaaaa attggtcaa 
      agaaatata tagatgtgt ggcctgtta atttcttaa 
      atccgggcg agatggatg cgtttgata cgacttcgt 
      tgtttatga tgcagttgg cgtgtattg ttaacatgg 
      tgccccttt ggagtcgcc ttaaactgc gtctgtgta 
      tttgtgacg aggcgagac agactcgct ttacattaa 
      gtccgaacg ggcaaagtc acatggaga agttaaatt 
      tgttgttga agagattga ggattgcgg cggtgccgt 
      ttccagttc ccgggtgga gtaatgtga ttgaatcgc 
      acggaaatc acatggaaa catacttaa aaagcgtta 
      ataatttga tattgcaac aaacttcag acacggtag 
      tgaccttta tataccttc caccgcccc caaatgacg 
      attctgagg cagaggcct attaccaaa aattggaca 
      ttatctcac gggaccttc ccccgacga tgtttgttc 
      acggcgtca gcacagtta cgtccacaa ctttataat 
      actagttga aatgcaagt atttcgaga acaaaaata 
      tttgtacaa tcttaatat ttttatacg cctatacaa 
      atttttaag acatatctt tactgcctt atgtattat 
      aataagata ctgtgatgt ttatgtaag gataataaa 
      aaaataata ggtca
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