| General information | |||
| TC014703 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 13583911..13633293 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC014703 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02807 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC014703 RA
MRLKSVDGP LSIHNGNSN DILNSPKRI NTLQNSNVT NFTSNLTTT TEYEDITGL TQRDNETLN NINSTQEKV QWCNPSQRT KKRELTNNA NNRNQLLNI NNTLAASLR TTRLGTASS IYSESSPDD SLLDFEDVH QSVSSADTS PQDEDRGFD ISDSDSSEE SNLSNRGTA TLQRRGIVN PNYPGFQHL AHTLDYSIK ASSDTDFTD DDFECESLN TAKTDVNNI NNNNVEDTD YPIDHIDSV NRLDSVENI QKVFYDKPV FNIQEECGN RSDSETSGN SNQTSDEDT EISVAQKLE LNIKTEENL TPIIGDFGK EVEQEFGRI SLENLEQAV VQELDDTIE KLNDAINTQ LSPIKYNIQ PNFAEPKLE PQPLVLNDT KTVKLTNMP LLTEARQSE PVIMKTKIC DAFLSNAQE AIASNLFEK DLDYARKDS NRELEEIEY QIKKLKSDN KHLLDEPEK FNKEDITKD YKEKDDDSA VPRKKEKID YYVKKRKDY NQQFGSLIT FPRRECGAR NRDVLNRRS VPMAREKKR TSPEVLGGF DVYNIETAM PKIDLEAIE SHLRAAREE ERRVSAPQK PHQHVCQVH VINTHV
- >TC014703 RB
MRCENLIRK DCHRNVNKP HDYLKSTNN GISPTWGIK VGKKPVLSR AEGRKEGSM RLKSVDGPL SIHNGNSND ILNSPKRIN TLQNSNVTN FTSNLTTTT EYEDITGLT QRDNETLNN INSTQEKVQ WCNPSQRTK KRELTNNAN NRNQLLNIN NTLAASLRT TRLGTASSI YSESSPDDS LLDFEDVHQ SVSSADTSP QDEDRGFDI SDSDSSEES NLSNRGTAT LQRRGIVNP NYPGFQHLA HTLDYSIKA SSDTDFTDD DFECESLNT AKTDVNNIN NNNVEDTDY PIDHIDSVN RLDSVENIQ KVFYDKPVF NIQEECGNR SDSETSGNS NQTSDEDTE ISVAQKLEL NIKTEENLT PIIGDFGKE VEQEFGRIS LENLEQAVV QELDDTIEK LNDAINTQL SPIKYNIQP NFAEPKLEP QPLVLNDTK TVKLTNMPL LTEARQSEP VIMKTKICD AFLSNAQEA IASNLFEKD LDYARKDSN RELEEIEYQ IKKLKSDNK HLLDEPEKF NKEDITKDY KEKDDDSAV PRKKEKIDY YVKKRKDYN QQFGSLITF PRRECGARN RDVLNRRSV PMAREKKRT SPEVLGGFD VYNIETAMP KIDLEAIES HLRAAREEE RRVSAPQKP HQHVCQVHV INTHV
- >TC014703 RA
atgcggttg aagtccgtg gacggaccg ctgtccatt cataatggc aacagcaac gacatacta aactcccct aaacggatt aacacgcta cagaacagc aacgtaaca aatttcact tcgaatctc acgaccaca actgaatat gaggacatc accggactc acacaacgc gataatgag acactaaac aatatcaac agcacccaa gagaaagtg caatggtgc aacccttct caaaggacg aagaagcga gagctaacc aacaacgcc aacaacaga aaccagttg ctcaatatc aacaacaca ctggctgct tccttaagg accacgagg ctcggcacc gcctcttcc atctattcg gagtcctcg ccagatgat agtcttcta gactttgag gacgtccat cagagcgtc tcgtccgcc gacacgtct ccccaggat gaggaccgc ggtttcgat atttccgac tccgactct tccgaagag agcaacttg agtaatcgc ggcacagcg accctccag cgcagaggc atagtgaac ccgaactat cccggcttt cagcacctc gctcacaca ctcgattac agcattaaa gcctcgtca gacaccgat ttcacagac gacgacttc gaatgcgaa tcactaaac accgccaaa accgacgtc aacaacatc aacaacaac aatgtggaa gacacggat tatccaatc gatcacatc gacagcgtc aaccgacta gacagcgtc gagaacatc cagaaggtc ttctacgac aagccggtg ttcaacatc caggaagaa tgcggcaac agaagcgac tcggaaacg agcggaaac tccaatcaa acaagcgac gaggacacg gaaattagc gtcgcgcaa aaactcgaa ctcaacatc aaaacagag gaaaacctc acgccgatc atcggcgat ttcggaaaa gaagtcgag caggagttt ggacgaatc tccctcgaa aacctcgaa caagccgtc gttcaagaa ctcgacgac acaatcgag aagttgaac gacgcgatc aacacgcaa ctatccccc atcaagtac aacatccag ccaaatttc gcggaacca aaactcgaa ccccagcct ctagtccta aacgacacc aaaaccgtc aaactaaca aacatgcct ttactgact gaagccaga caatcggag cccgtgatc atgaaaacg aaaatctgc gacgctttc ctctcaaac gcccaggaa gcgatcgcg tcaaacctc ttcgaaaag gacttggac tacgcccgg aaagactcc aaccgcgag ctcgaagaa atcgaatac caaataaaa aaactcaag tccgacaac aagcacctc ctcgacgag cccgagaag ttcaacaag gaggacatc acgaaggac tacaaggag aaggacgac gacagtgcg gtgccccgg aagaaggaa aaaatcgac tattacgtg aaaaaacga aaagactat aatcaacag tttggcagt ttgataacg ttcccgagg cgcgaatgc ggcgccaga aaccgcgat gtgctcaat aggaggtcg gttccgatg gctagggag aaaaaacgg acctcgccg gaagttcta ggcggtttc gacgtgtac aacatcgag accgcaatg cccaaaatc gacctggag gccatcgag tcccacctg cgagccgct cgcgaggag gaacgaagg gtgagtgca ccccaaaaa ccccatcaa catgtgtgt caagtgcac gtcataaac acacatgtg tag
- >TC014703 RB
atgcgctgt gagaatcta atccggaag gactgccac cggaacgtg aataagccc catgactat ctcaaatcg acgaataat ggaatctct ccgacttgg ggtatcaaa gtcgggaag aagcccgtt ttaagccga gcagagggg cgtaaggaa ggaagtatg cggttgaag tccgtggac ggaccgctg tccattcat aatggcaac agcaacgac atactaaac tcccctaaa cggattaac acgctacag aacagcaac gtaacaaat ttcacttcg aatctcacg accacaact gaatatgag gacatcacc ggactcaca caacgcgat aatgagaca ctaaacaat atcaacagc acccaagag aaagtgcaa tggtgcaac ccttctcaa aggacgaag aagcgagag ctaaccaac aacgccaac aacagaaac cagttgctc aatatcaac aacacactg gctgcttcc ttaaggacc acgaggctc ggcaccgcc tcttccatc tattcggag tcctcgcca gatgatagt cttctagac tttgaggac gtccatcag agcgtctcg tccgccgac acgtctccc caggatgag gaccgcggt ttcgatatt tccgactcc gactcttcc gaagagagc aacttgagt aatcgcggc acagcgacc ctccagcgc agaggcata gtgaacccg aactatccc ggctttcag cacctcgct cacacactc gattacagc attaaagcc tcgtcagac accgatttc acagacgac gacttcgaa tgcgaatca ctaaacacc gccaaaacc gacgtcaac aacatcaac aacaacaat gtggaagac acggattat ccaatcgat cacatcgac agcgtcaac cgactagac agcgtcgag aacatccag aaggtcttc tacgacaag ccggtgttc aacatccag gaagaatgc ggcaacaga agcgactcg gaaacgagc ggaaactcc aatcaaaca agcgacgag gacacggaa attagcgtc gcgcaaaaa ctcgaactc aacatcaaa acagaggaa aacctcacg ccgatcatc ggcgatttc ggaaaagaa gtcgagcag gagtttgga cgaatctcc ctcgaaaac ctcgaacaa gccgtcgtt caagaactc gacgacaca atcgagaag ttgaacgac gcgatcaac acgcaacta tcccccatc aagtacaac atccagcca aatttcgcg gaaccaaaa ctcgaaccc cagcctcta gtcctaaac gacaccaaa accgtcaaa ctaacaaac atgccttta ctgactgaa gccagacaa tcggagccc gtgatcatg aaaacgaaa atctgcgac gctttcctc tcaaacgcc caggaagcg atcgcgtca aacctcttc gaaaaggac ttggactac gcccggaaa gactccaac cgcgagctc gaagaaatc gaataccaa ataaaaaaa ctcaagtcc gacaacaag cacctcctc gacgagccc gagaagttc aacaaggag gacatcacg aaggactac aaggagaag gacgacgac agtgcggtg ccccggaag aaggaaaaa atcgactat tacgtgaaa aaacgaaaa gactataat caacagttt ggcagtttg ataacgttc ccgaggcgc gaatgcggc gccagaaac cgcgatgtg ctcaatagg aggtcggtt ccgatggct agggagaaa aaacggacc tcgccggaa gttctaggc ggtttcgac gtgtacaac atcgagacc gcaatgccc aaaatcgac ctggaggcc atcgagtcc cacctgcga gccgctcgc gaggaggaa cgaagggtg agtgcaccc caaaaaccc catcaacat gtgtgtcaa gtgcacgtc ataaacaca catgtgtag
- >TC014703 RA
ctgcaggac aacacacgt accgggtgc ggactgata gtgttacat aacagtggc ttgataccg gaatttccg ccaaaatgg accgagacg cgctcattg gaaggaagt atgcggttg aagtccgtg gacggaccg ctgtccatt cataatggc aacagcaac gacatacta aactcccct aaacggatt aacacgcta cagaacagc aacgtaaca aatttcact tcgaatctc acgaccaca actgaatat gaggacatc accggactc acacaacgc gataatgag acactaaac aatatcaac agcacccaa gagaaagtg caatggtgc aacccttct caaaggacg aagaagcga gagctaacc aacaacgcc aacaacaga aaccagttg ctcaatatc aacaacaca ctggctgct tccttaagg accacgagg ctcggcacc gcctcttcc atctattcg gagtcctcg ccagatgat agtcttcta gactttgag gacgtccat cagagcgtc tcgtccgcc gacacgtct ccccaggat gaggaccgc ggtttcgat atttccgac tccgactct tccgaagag agcaacttg agtaatcgc ggcacagcg accctccag cgcagaggc atagtgaac ccgaactat cccggcttt cagcacctc gctcacaca ctcgattac agcattaaa gcctcgtca gacaccgat ttcacagac gacgacttc gaatgcgaa tcactaaac accgccaaa accgacgtc aacaacatc aacaacaac aatgtggaa gacacggat tatccaatc gatcacatc gacagcgtc aaccgacta gacagcgtc gagaacatc cagaaggtc ttctacgac aagccggtg ttcaacatc caggaagaa tgcggcaac agaagcgac tcggaaacg agcggaaac tccaatcaa acaagcgac gaggacacg gaaattagc gtcgcgcaa aaactcgaa ctcaacatc aaaacagag gaaaacctc acgccgatc atcggcgat ttcggaaaa gaagtcgag caggagttt ggacgaatc tccctcgaa aacctcgaa caagccgtc gttcaagaa ctcgacgac acaatcgag aagttgaac gacgcgatc aacacgcaa ctatccccc atcaagtac aacatccag ccaaatttc gcggaacca aaactcgaa ccccagcct ctagtccta aacgacacc aaaaccgtc aaactaaca aacatgcct ttactgact gaagccaga caatcggag cccgtgatc atgaaaacg aaaatctgc gacgctttc ctctcaaac gcccaggaa gcgatcgcg tcaaacctc ttcgaaaag gacttggac tacgcccgg aaagactcc aaccgcgag ctcgaagaa atcgaatac caaataaaa aaactcaag tccgacaac aagcacctc ctcgacgag cccgagaag ttcaacaag gaggacatc acgaaggac tacaaggag aaggacgac gacagtgcg gtgccccgg aagaaggaa aaaatcgac tattacgtg aaaaaacga aaagactat aatcaacag tttggcagt ttgataacg ttcccgagg cgcgaatgc ggcgccaga aaccgcgat gtgctcaat aggaggtcg gttccgatg gctagggag aaaaaacgg acctcgccg gaagttcta ggcggtttc gacgtgtac aacatcgag accgcaatg cccaaaatc gacctggag gccatcgag tcccacctg cgagccgct cgcgaggag gaacgaagg gtgagtgca ccccaaaaa ccccatcaa catgtgtgt caagtgcac gtcataaac acacatgtg tagatcaca tctcctgca ctgagccta atctctcaa ttacatgcg gcagcaatc ggccaacgc gagttcgtt tagactaat ttatttcgc gaactgatg tcattaaat taaaagcgg aatgtttcg tgtcggaat ttttagcac gaatcgatg cattgtgct ggttattta attgtgatg gatgcgagc aaggtgagg aacacgctg aacttgtca cattgtaaa taaacaatg atatgttat
- >TC014703 RB
cattagaac ttcctatgc gctgtgaga atctaatcc ggaaggact gccaccgga acgtgaata agccccatg actatctca aatcgacga ataatggaa tctctccga cttggggta tcaaagtcg ggaagaagc ccgttttaa gccgagcag aggggcgta aggaaggaa gtatgcggt tgaagtccg tggacggac cgctgtcca ttcataatg gcaacagca acgacatac taaactccc ctaaacgga ttaacacgc tacagaaca gcaacgtaa caaatttca cttcgaatc tcacgacca caactgaat atgaggaca tcaccggac tcacacaac gcgataatg agacactaa acaatatca acagcaccc aagagaaag tgcaatggt gcaaccctt ctcaaagga cgaagaagc gagagctaa ccaacaacg ccaacaaca gaaaccagt tgctcaata tcaacaaca cactggctg cttccttaa ggaccacga ggctcggca ccgcctctt ccatctatt cggagtcct cgccagatg atagtcttc tagactttg aggacgtcc atcagagcg tctcgtccg ccgacacgt ctccccagg atgaggacc gcggtttcg atatttccg actccgact cttccgaag agagcaact tgagtaatc gcggcacag cgaccctcc agcgcagag gcatagtga acccgaact atcccggct ttcagcacc tcgctcaca cactcgatt acagcatta aagcctcgt cagacaccg atttcacag acgacgact tcgaatgcg aatcactaa acaccgcca aaaccgacg tcaacaaca tcaacaaca acaatgtgg aagacacgg attatccaa tcgatcaca tcgacagcg tcaaccgac tagacagcg tcgagaaca tccagaagg tcttctacg acaagccgg tgttcaaca tccaggaag aatgcggca acagaagcg actcggaaa cgagcggaa actccaatc aaacaagcg acgaggaca cggaaatta gcgtcgcgc aaaaactcg aactcaaca tcaaaacag aggaaaacc tcacgccga tcatcggcg atttcggaa aagaagtcg agcaggagt ttggacgaa tctccctcg aaaacctcg aacaagccg tcgttcaag aactcgacg acacaatcg agaagttga acgacgcga tcaacacgc aactatccc ccatcaagt acaacatcc agccaaatt tcgcggaac caaaactcg aaccccagc ctctagtcc taaacgaca ccaaaaccg tcaaactaa caaacatgc ctttactga ctgaagcca gacaatcgg agcccgtga tcatgaaaa cgaaaatct gcgacgctt tcctctcaa acgcccagg aagcgatcg cgtcaaacc tcttcgaaa aggacttgg actacgccc ggaaagact ccaaccgcg agctcgaag aaatcgaat accaaataa aaaaactca agtccgaca acaagcacc tcctcgacg agcccgaga agttcaaca aggaggaca tcacgaagg actacaagg agaaggacg acgacagtg cggtgcccc ggaagaagg aaaaaatcg actattacg tgaaaaaac gaaaagact ataatcaac agtttggca gtttgataa cgttcccga ggcgcgaat gcggcgcca gaaaccgcg atgtgctca ataggaggt cggttccga tggctaggg agaaaaaac ggacctcgc cggaagttc taggcggtt tcgacgtgt acaacatcg agaccgcaa tgcccaaaa tcgacctgg aggccatcg agtcccacc tgcgagccg ctcgcgagg aggaacgaa gggtgagtg caccccaaa aaccccatc aacatgtgt gtcaagtgc acgtcataa acacacatg tgtagatca catctcctg cactgagcc taatctctc aattacatg cggcagcaa tcggccaac gcgagttcg tttagacta atttatttc gcgaactga tgtcattaa attaaaagc ggaatgttt cgtgtcgga atttttagc acgaatcga tgcattgtg ctggttatt taattgtga tggatgcga gcaaggtga ggaacacgc tgaacttgt cacattgta aataaacaa tgatatgtt at
