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Gene information
General information
TC014703 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13583911..13633293
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014703
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02807

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014703 RA
      MRLKSVDGP LSIHNGNSN DILNSPKRI NTLQNSNVT 
      NFTSNLTTT TEYEDITGL TQRDNETLN NINSTQEKV 
      QWCNPSQRT KKRELTNNA NNRNQLLNI NNTLAASLR 
      TTRLGTASS IYSESSPDD SLLDFEDVH QSVSSADTS 
      PQDEDRGFD ISDSDSSEE SNLSNRGTA TLQRRGIVN 
      PNYPGFQHL AHTLDYSIK ASSDTDFTD DDFECESLN 
      TAKTDVNNI NNNNVEDTD YPIDHIDSV NRLDSVENI 
      QKVFYDKPV FNIQEECGN RSDSETSGN SNQTSDEDT 
      EISVAQKLE LNIKTEENL TPIIGDFGK EVEQEFGRI 
      SLENLEQAV VQELDDTIE KLNDAINTQ LSPIKYNIQ 
      PNFAEPKLE PQPLVLNDT KTVKLTNMP LLTEARQSE 
      PVIMKTKIC DAFLSNAQE AIASNLFEK DLDYARKDS 
      NRELEEIEY QIKKLKSDN KHLLDEPEK FNKEDITKD 
      YKEKDDDSA VPRKKEKID YYVKKRKDY NQQFGSLIT 
      FPRRECGAR NRDVLNRRS VPMAREKKR TSPEVLGGF 
      DVYNIETAM PKIDLEAIE SHLRAAREE ERRVSAPQK 
      PHQHVCQVH VINTHV
    • >TC014703 RB
      MRCENLIRK DCHRNVNKP HDYLKSTNN GISPTWGIK 
      VGKKPVLSR AEGRKEGSM RLKSVDGPL SIHNGNSND 
      ILNSPKRIN TLQNSNVTN FTSNLTTTT EYEDITGLT 
      QRDNETLNN INSTQEKVQ WCNPSQRTK KRELTNNAN 
      NRNQLLNIN NTLAASLRT TRLGTASSI YSESSPDDS 
      LLDFEDVHQ SVSSADTSP QDEDRGFDI SDSDSSEES 
      NLSNRGTAT LQRRGIVNP NYPGFQHLA HTLDYSIKA 
      SSDTDFTDD DFECESLNT AKTDVNNIN NNNVEDTDY 
      PIDHIDSVN RLDSVENIQ KVFYDKPVF NIQEECGNR 
      SDSETSGNS NQTSDEDTE ISVAQKLEL NIKTEENLT 
      PIIGDFGKE VEQEFGRIS LENLEQAVV QELDDTIEK 
      LNDAINTQL SPIKYNIQP NFAEPKLEP QPLVLNDTK 
      TVKLTNMPL LTEARQSEP VIMKTKICD AFLSNAQEA 
      IASNLFEKD LDYARKDSN RELEEIEYQ IKKLKSDNK 
      HLLDEPEKF NKEDITKDY KEKDDDSAV PRKKEKIDY 
      YVKKRKDYN QQFGSLITF PRRECGARN RDVLNRRSV 
      PMAREKKRT SPEVLGGFD VYNIETAMP KIDLEAIES 
      HLRAAREEE RRVSAPQKP HQHVCQVHV INTHV
    CDS sequence
    • >TC014703 RA
      atgcggttg aagtccgtg gacggaccg ctgtccatt 
      cataatggc aacagcaac gacatacta aactcccct 
      aaacggatt aacacgcta cagaacagc aacgtaaca 
      aatttcact tcgaatctc acgaccaca actgaatat 
      gaggacatc accggactc acacaacgc gataatgag 
      acactaaac aatatcaac agcacccaa gagaaagtg 
      caatggtgc aacccttct caaaggacg aagaagcga 
      gagctaacc aacaacgcc aacaacaga aaccagttg 
      ctcaatatc aacaacaca ctggctgct tccttaagg 
      accacgagg ctcggcacc gcctcttcc atctattcg 
      gagtcctcg ccagatgat agtcttcta gactttgag 
      gacgtccat cagagcgtc tcgtccgcc gacacgtct 
      ccccaggat gaggaccgc ggtttcgat atttccgac 
      tccgactct tccgaagag agcaacttg agtaatcgc 
      ggcacagcg accctccag cgcagaggc atagtgaac 
      ccgaactat cccggcttt cagcacctc gctcacaca 
      ctcgattac agcattaaa gcctcgtca gacaccgat 
      ttcacagac gacgacttc gaatgcgaa tcactaaac 
      accgccaaa accgacgtc aacaacatc aacaacaac 
      aatgtggaa gacacggat tatccaatc gatcacatc 
      gacagcgtc aaccgacta gacagcgtc gagaacatc 
      cagaaggtc ttctacgac aagccggtg ttcaacatc 
      caggaagaa tgcggcaac agaagcgac tcggaaacg 
      agcggaaac tccaatcaa acaagcgac gaggacacg 
      gaaattagc gtcgcgcaa aaactcgaa ctcaacatc 
      aaaacagag gaaaacctc acgccgatc atcggcgat 
      ttcggaaaa gaagtcgag caggagttt ggacgaatc 
      tccctcgaa aacctcgaa caagccgtc gttcaagaa 
      ctcgacgac acaatcgag aagttgaac gacgcgatc 
      aacacgcaa ctatccccc atcaagtac aacatccag 
      ccaaatttc gcggaacca aaactcgaa ccccagcct 
      ctagtccta aacgacacc aaaaccgtc aaactaaca 
      aacatgcct ttactgact gaagccaga caatcggag 
      cccgtgatc atgaaaacg aaaatctgc gacgctttc 
      ctctcaaac gcccaggaa gcgatcgcg tcaaacctc 
      ttcgaaaag gacttggac tacgcccgg aaagactcc 
      aaccgcgag ctcgaagaa atcgaatac caaataaaa 
      aaactcaag tccgacaac aagcacctc ctcgacgag 
      cccgagaag ttcaacaag gaggacatc acgaaggac 
      tacaaggag aaggacgac gacagtgcg gtgccccgg 
      aagaaggaa aaaatcgac tattacgtg aaaaaacga 
      aaagactat aatcaacag tttggcagt ttgataacg 
      ttcccgagg cgcgaatgc ggcgccaga aaccgcgat 
      gtgctcaat aggaggtcg gttccgatg gctagggag 
      aaaaaacgg acctcgccg gaagttcta ggcggtttc 
      gacgtgtac aacatcgag accgcaatg cccaaaatc 
      gacctggag gccatcgag tcccacctg cgagccgct 
      cgcgaggag gaacgaagg gtgagtgca ccccaaaaa 
      ccccatcaa catgtgtgt caagtgcac gtcataaac 
      acacatgtg tag
    • >TC014703 RB
      atgcgctgt gagaatcta atccggaag gactgccac 
      cggaacgtg aataagccc catgactat ctcaaatcg 
      acgaataat ggaatctct ccgacttgg ggtatcaaa 
      gtcgggaag aagcccgtt ttaagccga gcagagggg 
      cgtaaggaa ggaagtatg cggttgaag tccgtggac 
      ggaccgctg tccattcat aatggcaac agcaacgac 
      atactaaac tcccctaaa cggattaac acgctacag 
      aacagcaac gtaacaaat ttcacttcg aatctcacg 
      accacaact gaatatgag gacatcacc ggactcaca 
      caacgcgat aatgagaca ctaaacaat atcaacagc 
      acccaagag aaagtgcaa tggtgcaac ccttctcaa 
      aggacgaag aagcgagag ctaaccaac aacgccaac 
      aacagaaac cagttgctc aatatcaac aacacactg 
      gctgcttcc ttaaggacc acgaggctc ggcaccgcc 
      tcttccatc tattcggag tcctcgcca gatgatagt 
      cttctagac tttgaggac gtccatcag agcgtctcg 
      tccgccgac acgtctccc caggatgag gaccgcggt 
      ttcgatatt tccgactcc gactcttcc gaagagagc 
      aacttgagt aatcgcggc acagcgacc ctccagcgc 
      agaggcata gtgaacccg aactatccc ggctttcag 
      cacctcgct cacacactc gattacagc attaaagcc 
      tcgtcagac accgatttc acagacgac gacttcgaa 
      tgcgaatca ctaaacacc gccaaaacc gacgtcaac 
      aacatcaac aacaacaat gtggaagac acggattat 
      ccaatcgat cacatcgac agcgtcaac cgactagac 
      agcgtcgag aacatccag aaggtcttc tacgacaag 
      ccggtgttc aacatccag gaagaatgc ggcaacaga 
      agcgactcg gaaacgagc ggaaactcc aatcaaaca 
      agcgacgag gacacggaa attagcgtc gcgcaaaaa 
      ctcgaactc aacatcaaa acagaggaa aacctcacg 
      ccgatcatc ggcgatttc ggaaaagaa gtcgagcag 
      gagtttgga cgaatctcc ctcgaaaac ctcgaacaa 
      gccgtcgtt caagaactc gacgacaca atcgagaag 
      ttgaacgac gcgatcaac acgcaacta tcccccatc 
      aagtacaac atccagcca aatttcgcg gaaccaaaa 
      ctcgaaccc cagcctcta gtcctaaac gacaccaaa 
      accgtcaaa ctaacaaac atgccttta ctgactgaa 
      gccagacaa tcggagccc gtgatcatg aaaacgaaa 
      atctgcgac gctttcctc tcaaacgcc caggaagcg 
      atcgcgtca aacctcttc gaaaaggac ttggactac 
      gcccggaaa gactccaac cgcgagctc gaagaaatc 
      gaataccaa ataaaaaaa ctcaagtcc gacaacaag 
      cacctcctc gacgagccc gagaagttc aacaaggag 
      gacatcacg aaggactac aaggagaag gacgacgac 
      agtgcggtg ccccggaag aaggaaaaa atcgactat 
      tacgtgaaa aaacgaaaa gactataat caacagttt 
      ggcagtttg ataacgttc ccgaggcgc gaatgcggc 
      gccagaaac cgcgatgtg ctcaatagg aggtcggtt 
      ccgatggct agggagaaa aaacggacc tcgccggaa 
      gttctaggc ggtttcgac gtgtacaac atcgagacc 
      gcaatgccc aaaatcgac ctggaggcc atcgagtcc 
      cacctgcga gccgctcgc gaggaggaa cgaagggtg 
      agtgcaccc caaaaaccc catcaacat gtgtgtcaa 
      gtgcacgtc ataaacaca catgtgtag
    mRNA sequence
    • >TC014703 RA
      ctgcaggac aacacacgt accgggtgc ggactgata 
      gtgttacat aacagtggc ttgataccg gaatttccg 
      ccaaaatgg accgagacg cgctcattg gaaggaagt 
      atgcggttg aagtccgtg gacggaccg ctgtccatt 
      cataatggc aacagcaac gacatacta aactcccct 
      aaacggatt aacacgcta cagaacagc aacgtaaca 
      aatttcact tcgaatctc acgaccaca actgaatat 
      gaggacatc accggactc acacaacgc gataatgag 
      acactaaac aatatcaac agcacccaa gagaaagtg 
      caatggtgc aacccttct caaaggacg aagaagcga 
      gagctaacc aacaacgcc aacaacaga aaccagttg 
      ctcaatatc aacaacaca ctggctgct tccttaagg 
      accacgagg ctcggcacc gcctcttcc atctattcg 
      gagtcctcg ccagatgat agtcttcta gactttgag 
      gacgtccat cagagcgtc tcgtccgcc gacacgtct 
      ccccaggat gaggaccgc ggtttcgat atttccgac 
      tccgactct tccgaagag agcaacttg agtaatcgc 
      ggcacagcg accctccag cgcagaggc atagtgaac 
      ccgaactat cccggcttt cagcacctc gctcacaca 
      ctcgattac agcattaaa gcctcgtca gacaccgat 
      ttcacagac gacgacttc gaatgcgaa tcactaaac 
      accgccaaa accgacgtc aacaacatc aacaacaac 
      aatgtggaa gacacggat tatccaatc gatcacatc 
      gacagcgtc aaccgacta gacagcgtc gagaacatc 
      cagaaggtc ttctacgac aagccggtg ttcaacatc 
      caggaagaa tgcggcaac agaagcgac tcggaaacg 
      agcggaaac tccaatcaa acaagcgac gaggacacg 
      gaaattagc gtcgcgcaa aaactcgaa ctcaacatc 
      aaaacagag gaaaacctc acgccgatc atcggcgat 
      ttcggaaaa gaagtcgag caggagttt ggacgaatc 
      tccctcgaa aacctcgaa caagccgtc gttcaagaa 
      ctcgacgac acaatcgag aagttgaac gacgcgatc 
      aacacgcaa ctatccccc atcaagtac aacatccag 
      ccaaatttc gcggaacca aaactcgaa ccccagcct 
      ctagtccta aacgacacc aaaaccgtc aaactaaca 
      aacatgcct ttactgact gaagccaga caatcggag 
      cccgtgatc atgaaaacg aaaatctgc gacgctttc 
      ctctcaaac gcccaggaa gcgatcgcg tcaaacctc 
      ttcgaaaag gacttggac tacgcccgg aaagactcc 
      aaccgcgag ctcgaagaa atcgaatac caaataaaa 
      aaactcaag tccgacaac aagcacctc ctcgacgag 
      cccgagaag ttcaacaag gaggacatc acgaaggac 
      tacaaggag aaggacgac gacagtgcg gtgccccgg 
      aagaaggaa aaaatcgac tattacgtg aaaaaacga 
      aaagactat aatcaacag tttggcagt ttgataacg 
      ttcccgagg cgcgaatgc ggcgccaga aaccgcgat 
      gtgctcaat aggaggtcg gttccgatg gctagggag 
      aaaaaacgg acctcgccg gaagttcta ggcggtttc 
      gacgtgtac aacatcgag accgcaatg cccaaaatc 
      gacctggag gccatcgag tcccacctg cgagccgct 
      cgcgaggag gaacgaagg gtgagtgca ccccaaaaa 
      ccccatcaa catgtgtgt caagtgcac gtcataaac 
      acacatgtg tagatcaca tctcctgca ctgagccta 
      atctctcaa ttacatgcg gcagcaatc ggccaacgc 
      gagttcgtt tagactaat ttatttcgc gaactgatg 
      tcattaaat taaaagcgg aatgtttcg tgtcggaat 
      ttttagcac gaatcgatg cattgtgct ggttattta 
      attgtgatg gatgcgagc aaggtgagg aacacgctg 
      aacttgtca cattgtaaa taaacaatg atatgttat
    • >TC014703 RB
      cattagaac ttcctatgc gctgtgaga atctaatcc 
      ggaaggact gccaccgga acgtgaata agccccatg 
      actatctca aatcgacga ataatggaa tctctccga 
      cttggggta tcaaagtcg ggaagaagc ccgttttaa 
      gccgagcag aggggcgta aggaaggaa gtatgcggt 
      tgaagtccg tggacggac cgctgtcca ttcataatg 
      gcaacagca acgacatac taaactccc ctaaacgga 
      ttaacacgc tacagaaca gcaacgtaa caaatttca 
      cttcgaatc tcacgacca caactgaat atgaggaca 
      tcaccggac tcacacaac gcgataatg agacactaa 
      acaatatca acagcaccc aagagaaag tgcaatggt 
      gcaaccctt ctcaaagga cgaagaagc gagagctaa 
      ccaacaacg ccaacaaca gaaaccagt tgctcaata 
      tcaacaaca cactggctg cttccttaa ggaccacga 
      ggctcggca ccgcctctt ccatctatt cggagtcct 
      cgccagatg atagtcttc tagactttg aggacgtcc 
      atcagagcg tctcgtccg ccgacacgt ctccccagg 
      atgaggacc gcggtttcg atatttccg actccgact 
      cttccgaag agagcaact tgagtaatc gcggcacag 
      cgaccctcc agcgcagag gcatagtga acccgaact 
      atcccggct ttcagcacc tcgctcaca cactcgatt 
      acagcatta aagcctcgt cagacaccg atttcacag 
      acgacgact tcgaatgcg aatcactaa acaccgcca 
      aaaccgacg tcaacaaca tcaacaaca acaatgtgg 
      aagacacgg attatccaa tcgatcaca tcgacagcg 
      tcaaccgac tagacagcg tcgagaaca tccagaagg 
      tcttctacg acaagccgg tgttcaaca tccaggaag 
      aatgcggca acagaagcg actcggaaa cgagcggaa 
      actccaatc aaacaagcg acgaggaca cggaaatta 
      gcgtcgcgc aaaaactcg aactcaaca tcaaaacag 
      aggaaaacc tcacgccga tcatcggcg atttcggaa 
      aagaagtcg agcaggagt ttggacgaa tctccctcg 
      aaaacctcg aacaagccg tcgttcaag aactcgacg 
      acacaatcg agaagttga acgacgcga tcaacacgc 
      aactatccc ccatcaagt acaacatcc agccaaatt 
      tcgcggaac caaaactcg aaccccagc ctctagtcc 
      taaacgaca ccaaaaccg tcaaactaa caaacatgc 
      ctttactga ctgaagcca gacaatcgg agcccgtga 
      tcatgaaaa cgaaaatct gcgacgctt tcctctcaa 
      acgcccagg aagcgatcg cgtcaaacc tcttcgaaa 
      aggacttgg actacgccc ggaaagact ccaaccgcg 
      agctcgaag aaatcgaat accaaataa aaaaactca 
      agtccgaca acaagcacc tcctcgacg agcccgaga 
      agttcaaca aggaggaca tcacgaagg actacaagg 
      agaaggacg acgacagtg cggtgcccc ggaagaagg 
      aaaaaatcg actattacg tgaaaaaac gaaaagact 
      ataatcaac agtttggca gtttgataa cgttcccga 
      ggcgcgaat gcggcgcca gaaaccgcg atgtgctca 
      ataggaggt cggttccga tggctaggg agaaaaaac 
      ggacctcgc cggaagttc taggcggtt tcgacgtgt 
      acaacatcg agaccgcaa tgcccaaaa tcgacctgg 
      aggccatcg agtcccacc tgcgagccg ctcgcgagg 
      aggaacgaa gggtgagtg caccccaaa aaccccatc 
      aacatgtgt gtcaagtgc acgtcataa acacacatg 
      tgtagatca catctcctg cactgagcc taatctctc 
      aattacatg cggcagcaa tcggccaac gcgagttcg 
      tttagacta atttatttc gcgaactga tgtcattaa 
      attaaaagc ggaatgttt cgtgtcgga atttttagc 
      acgaatcga tgcattgtg ctggttatt taattgtga 
      tggatgcga gcaaggtga ggaacacgc tgaacttgt 
      cacattgta aataaacaa tgatatgtt at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY