Status
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Gene information
General information
TC033186
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014549,TC014548
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11513091..11517908
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033186
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02805

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033186 RA
      MGMITRFRG RLRSFHTIQ VDPEMQNEK IAETVCLLS 
      VLFLLPYCS RGHAPLLVS LGGWCVASY SFLVLPVLI 
      SANYKYPVR WLRKLFAKI PALLSKWSG PVWSFICRV 
      YAPLDRCEK MFMKMTNLS NLATQLVFF MMCDRVLLC 
      SFGGGNCPQ KRVTGLYSL MFYNVIAYC VSYIKELIE 
      KEDWSVTVN MTQHSNIKH VAMSATKIV LEWTKAITF 
      IITVTFMLL VFGLEQGLE HYQPTALYT LITWTYYMC 
      TERVFIELF PPFLVFLRL KHLEALESL YAPVILRYY 
      TVSVAAVLV LLLCFYGQV RFTFIAFYL TVYLRLKDM 
      VANCLKVLR QEQAVLGQF RHATEEEIE NCDDVCAVC 
      LSPMEKARV TPCHHLFHA SCLRQCLSA SQNCPMCKR 
      EYTFVH
    CDS sequence
    • >TC033186 RA
      atgggaatg ataaccagg ttccggggc cgactgcgg 
      agcttccac accatccag gtcgacccg gaaatgcaa 
      aacgaaaaa atcgccgag actgtgtgt ctgttgtcc 
      gttctgttc ctcctgcct tactgctcc agaggccat 
      gccccgctg ctcgtgagt ttggggggc tggtgcgtg 
      gcgagttac tcgttcctg gtgctcccg gtgctcatt 
      tcggccaac tacaagtac cctgtgagg tggttgcgg 
      aagttgttt gccaaaatc ccagctctg ttgagcaag 
      tggtcgggc cccgtttgg tcctttatt tgccgggtg 
      tatgctccg ctggaccgg tgcgaaaaa atgttcatg 
      aaaatgacg aatttgtca aatctggcc acacaactg 
      gtgttcttc atgatgtgc gaccgcgtg ctgctgtgc 
      tccttcggg ggcggcaac tgcccccag aagagggtc 
      accgggctg tactccctc atgttttac aacgtgatc 
      gcctattgc gtgtcgtac atcaaggag ctgatcgag 
      aaggaggac tggtctgtg accgtcaac atgacccag 
      cacagcaac atcaagcac gtggcgatg tccgccacc 
      aaaatcgtg ctcgaatgg accaaagcc ataacgttc 
      atcatcacc gtcaccttc atgctgctc gtcttcggc 
      ttggagcag ggcttggag cactaccag cccacggcc 
      ctgtacacg ctcatcacc tggacctac tacatgtgc 
      accgagagg gtcttcatc gagctgttc ccgccgttc 
      ctggtcttc ctgcggctg aagcacctg gaggccctg 
      gagtcgctc tacgccccg gtgattctg cgctactac 
      accgtgagt gtcgcggcc gttctggtg ctcttgttg 
      tgcttctac ggccaggtc aggttcacg ttcatcgcc 
      ttctacttg acggtgtat ctgagactc aaggacatg 
      gtggcgaac tgcctgaag gttttgagg caggagcag 
      gcggttctg gggcagttc aggcacgcc accgaggag 
      gagattgag aactgtgac gatgtctgt gctgtttgt 
      ttgagtccg atggagaag gcgagggtt acgccgtgc 
      catcactta ttccacgcc agttgtctc agacagtgc 
      ctgagcgcg tcgcagaac tgccccatg tgcaagagg 
      gagtacacg tttgtgcac tag
    mRNA sequence
    • >TC033186 RA
      tgggacata aaaatgagg tgggggccg ttccagaaa 
      gaaaaacat caacgtagg tgatcccaa taaagaaat 
      ttgcgagtc aacacaaag aacagtcag aaagcgaga 
      gctcgaaag cgcgttgca gttcatcca agtctattt 
      caagttgag ttaatagtg ttctagaag tttccgcca 
      cagtccgca caatgggaa tgataacca ggttccggg 
      gccgactgc ggagcttcc acaccatcc aggtcgacc 
      cggaaatgc aaaacgaaa aaatcgccg agactgtgt 
      gtctgttgt ccgttctgt tcctcctgc cttactgct 
      ccagaggcc atgccccgc tgctcgtga gtttggggg 
      gctggtgcg tggcgagtt actcgttcc tggtgctcc 
      cggtgctca tttcggcca actacaagt accctgtga 
      ggtggttgc ggaagttgt ttgccaaaa tcccagctc 
      tgttgagca agtggtcgg gccccgttt ggtccttta 
      tttgccggg tgtatgctc cgctggacc ggtgcgaaa 
      aaatgttca tgaaaatga cgaatttgt caaatctgg 
      ccacacaac tggtgttct tcatgatgt gcgaccgcg 
      tgctgctgt gctccttcg ggggcggca actgccccc 
      agaagaggg tcaccgggc tgtactccc tcatgtttt 
      acaacgtga tcgcctatt gcgtgtcgt acatcaagg 
      agctgatcg agaaggagg actggtctg tgaccgtca 
      acatgaccc agcacagca acatcaagc acgtggcga 
      tgtccgcca ccaaaatcg tgctcgaat ggaccaaag 
      ccataacgt tcatcatca ccgtcacct tcatgctgc 
      tcgtcttcg gcttggagc agggcttgg agcactacc 
      agcccacgg ccctgtaca cgctcatca cctggacct 
      actacatgt gcaccgaga gggtcttca tcgagctgt 
      tcccgccgt tcctggtct tcctgcggc tgaagcacc 
      tggaggccc tggagtcgc tctacgccc cggtgattc 
      tgcgctact acaccgtga gtgtcgcgg ccgttctgg 
      tgctcttgt tgtgcttct acggccagg tcaggttca 
      cgttcatcg ccttctact tgacggtgt atctgagac 
      tcaaggaca tggtggcga actgcctga aggttttga 
      ggcaggagc aggcggttc tggggcagt tcaggcacg 
      ccaccgagg aggagattg agaactgtg acgatgtct 
      gtgctgttt gtttgagtc cgatggaga aggcgaggg 
      ttacgccgt gccatcact tattccacg ccagttgtc 
      tcagacagt gcctgagcg cgtcgcaga actgcccca 
      tgtgcaaga gggagtaca cgtttgtgc actagggtt 
      agggatttt tgtacggtc gtactagct taatatttt 
      gtacgtgac taggatagg cgaggtaga ctagttttg 
      ctacgttta tatgtattt atttattaa tttatattt 
      tgtatgtga ataaaagta tcagaaaca t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY