Status
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Gene information
General information
TC033183
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013260
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 3560937..3572600
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033183
    iBeetle screen iB_02804
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033183 RA
      MSSMKQQRS SEKLSRKSK QTTDFSKVG GLANHIKIL 
      RDTIIVPLL HNNVYSHFN IKTPRGILF YGPPGTGKT 
      LVAGALATE LNKEGFGKV SFYQRKGAD VLDKWVGES 
      EKKLRTLFE NASKNRPSI IFFDELDGL APIRSVKND 
      HVHCSIVTT LLALMDGLD SVPGVIVIG ATNRIESVD 
      PALRRPGRF DRELYFPLP STEARKEIL QVHMQTWRH 
      RPSNQFISE LAEMTTGFC GSDLQALCA EALLCAMKR 
      QHPNIQKCL LGARVKIEA GEIRIDDSD FINARKNLI 
      PSSHKMGIR MRKLSPVIT PLLQKQLDM ILSRVNMLW 
      PHFSMQFYK YLIGSERYV GRVVLVGSN LQGLNAHLV 
      PAILQNYEH LPATFLDAA TLQHRSLVA NFCKNLPSI 
      LVIPRVDDL WSFLDANEQ TYIASLLEE VHAGLPILI 
      IATYEEELP LMLQNFFYN NTTVVINIE NPSDHEVEE 
      FFIPLFFGD SPHSLSTIL SGCKQSCKR KSNETLETL 
      NKGKGPRYV TSSKVYKLK PSGKITKSR SNIALKKTL 
      RLSPDKDLI RCPSKHFDE LRAVNTESL CTLCGTTEK 
      KKRLSLILT DLLQRSIRY FSRRRSPHE HTSSHRSIS 
      LLQNYGDSS DIDKQKIYS LWRRTVMKT AKDMPLAHL 
      EVLYDTISA CISIHKDDF QNLVKNLDD TLLEIEATK
    • >TC033183 RB
      MSSMKQQRS SEKLSRKSK QTTDFSKVG GLANHIKIL 
      RDTIIVPLL HNNVYSHFN IKTPRGILF YGPPGTGKT 
      LVAGALATE LNKEGFGKV SFYQRKGAD VLDKWVGES 
      EKKLRTLFE NASKNRPSI IFFDELDGL APIRSVKND 
      HVHCSIVTT LLALMDGLD SVPGVIVIG ATNRIESVD 
      PALRRPGRF DRELYFPLP STEARKEIL QVHMQTWRH 
      RPSNQFISE LAEMTTGFC GSDLQALCA EALLCAMKR 
      QHPNIQKCL LGARVKIEA GEIRIDDSD FINARKNLI 
      PSSHKMGIR MRKLSPVIT PLLQKQLDM ILSRVNMLW 
      PHFSMQFYK YLIGSERYV GRVVLVGSN LQGLNAHLV 
      PAILQNYEH LPATFLDAA TLQHRSLVA NFCKNLPSI 
      LVIPRVDDL WSFLDANEQ TYIASLLEE VHAGLPILI 
      IATYEEELP LMLQNFFYN NTTVVINIE NPSDHEVEE 
      FFIPLFFGD SPHSLSTIL SGCKQSCKR KSNETLETL 
      NKGKGPRYV TSSKVYKLK PSGKITKSR SNIALKKTL 
      RLSPDKDLI RCPSKHFDE LRAVNTESL CTLCGTTEK 
      KKRLSLILT DLLQRSIRY FSRRRSPHE HTSSHRSIS 
      LLQNYGDSS DIDKQKIYS LWRRTVMKT AKDMPLAHL 
      EVLYDTISA CISIHKDDF QNLVKVQFF QN
    CDS sequence
    • >TC033183 RA
      atgtctagt atgaagcaa cagaggtca tccgaaaaa 
      ttgagtagg aaatcaaaa caaacaacc gatttttca 
      aaagttggt ggtttggca aatcacatc aaaatttta 
      cgtgatacg ataatagtg cccctattg cacaacaac 
      gtttattcg cactttaac atcaaaaca ccccgcggt 
      attctcttc tacggacct ccaggcaca ggcaagact 
      ttggtcgct ggcgctctt gcaaccgaa ctcaacaaa 
      gagggattt ggcaaagtg agtttctac caaaggaaa 
      ggggccgat gttttggac aaatgggtc ggcgagtcg 
      gaaaagaaa ctccgaact ctatttgaa aacgcttcg 
      aaaaaccga ccttcgatt atatttttc gacgagctg 
      gacggactt gctccaatt cgttcggtc aaaaacgac 
      catgtccac tgcagcatt gtcacaacc cttttggcc 
      cttatggac gggcttgac agcgtccct ggggtcata 
      gtcataggt gcaacaaac cgaatcgag agcgtggat 
      ccagcgttg cgacgtcct ggccgtttt gaccgggaa 
      ttgtacttc cctttgccc tccaccgag gcccggaag 
      gaaatcttg caagtgcac atgcagacg tggagacac 
      cggccctcc aatcaattt atttcagaa ttggccgaa 
      atgaccacc ggcttctgc ggctctgat ttgcaagca 
      ctttgcgct gaggctcta ctatgtgcc atgaagaga 
      caacaccca aacatccaa aagtgcctc ctgggggcc 
      cgtgtcaaa atcgaagcc ggagaaatt aggatagac 
      gatagtgat tttatcaac gctcgcaaa aacttgatt 
      ccttcgtcg cataaaatg gggatcagg atgcgcaag 
      ttgagtcct gtcatcacc ccgttgttg caaaaacag 
      ttagatatg atcttgtca agggttaac atgttgtgg 
      ccccatttt tcgatgcag ttttacaag tatttgatt 
      gggagtgag cgttatgtt ggaagagtt gtgttggtt 
      gggtcaaat ttgcaaggg ttaaatgcg catctagtt 
      ccggctatt ttacaaaat tacgaacac ttaccggcg 
      acttttttg gacgctgcg accctacaa caccgaagc 
      cttgtagca aatttttgt aaaaatttg ccctcgatt 
      ttggtaatc ccccgagtg gacgacttg tggtcattt 
      ctggacgca aacgagcag acttacatt gcttcactt 
      ctcgaagag gtgcacgca ggtttgcca attttaata 
      atagcaacg tatgaagaa gagttgcct ttgatgtta 
      cagaatttc ttttataat aacaccact gtggttatc 
      aacattgag aacccctca gaccacgaa gtagaggaa 
      tttttcatt cccctgttc tttggggac tcgccacac 
      agcttaagc acaatctta tcaggttgc aagcaaagc 
      tgtaaaaga aaaagtaac gaaactttg gaaacatta 
      aataagggg aaaggcccg cgatacgtc acttcttca 
      aaagtgtac aaattaaaa ccatcaggg aaaattaca 
      aagtctcgt tcaaacatc gcattaaaa aaaactttg 
      agactcagc cccgataaa gatctcatt cgttgtccc 
      tccaaacat ttcgacgaa ctccgggca gttaacacc 
      gaaagtttg tgcacttta tgcggaact acggaaaaa 
      aagaagcgt ctttctctg attctgacc gatttgttg 
      caacgaagt attcgatac ttttccaga cgcaggtca 
      ccacatgag cacaccagc agtcacaga agcatcagt 
      cttttgcaa aattacggc gattcttcc gatattgat 
      aagcagaaa atttacagt ttgtggcga cgcactgtc 
      atgaaaaca gccaaagac atgcctttg gctcatttg 
      gaagtcttg tacgacacc atttcggcc tgcattagc 
      attcacaaa gacgatttt caaaattta gttaagaat 
      ttggatgac actcttctc gaaattgag gcaactaag 
      tag
    • >TC033183 RB
      atgtctagt atgaagcaa cagaggtca tccgaaaaa 
      ttgagtagg aaatcaaaa caaacaacc gatttttca 
      aaagttggt ggtttggca aatcacatc aaaatttta 
      cgtgatacg ataatagtg cccctattg cacaacaac 
      gtttattcg cactttaac atcaaaaca ccccgcggt 
      attctcttc tacggacct ccaggcaca ggcaagact 
      ttggtcgct ggcgctctt gcaaccgaa ctcaacaaa 
      gagggattt ggcaaagtg agtttctac caaaggaaa 
      ggggccgat gttttggac aaatgggtc ggcgagtcg 
      gaaaagaaa ctccgaact ctatttgaa aacgcttcg 
      aaaaaccga ccttcgatt atatttttc gacgagctg 
      gacggactt gctccaatt cgttcggtc aaaaacgac 
      catgtccac tgcagcatt gtcacaacc cttttggcc 
      cttatggac gggcttgac agcgtccct ggggtcata 
      gtcataggt gcaacaaac cgaatcgag agcgtggat 
      ccagcgttg cgacgtcct ggccgtttt gaccgggaa 
      ttgtacttc cctttgccc tccaccgag gcccggaag 
      gaaatcttg caagtgcac atgcagacg tggagacac 
      cggccctcc aatcaattt atttcagaa ttggccgaa 
      atgaccacc ggcttctgc ggctctgat ttgcaagca 
      ctttgcgct gaggctcta ctatgtgcc atgaagaga 
      caacaccca aacatccaa aagtgcctc ctgggggcc 
      cgtgtcaaa atcgaagcc ggagaaatt aggatagac 
      gatagtgat tttatcaac gctcgcaaa aacttgatt 
      ccttcgtcg cataaaatg gggatcagg atgcgcaag 
      ttgagtcct gtcatcacc ccgttgttg caaaaacag 
      ttagatatg atcttgtca agggttaac atgttgtgg 
      ccccatttt tcgatgcag ttttacaag tatttgatt 
      gggagtgag cgttatgtt ggaagagtt gtgttggtt 
      gggtcaaat ttgcaaggg ttaaatgcg catctagtt 
      ccggctatt ttacaaaat tacgaacac ttaccggcg 
      acttttttg gacgctgcg accctacaa caccgaagc 
      cttgtagca aatttttgt aaaaatttg ccctcgatt 
      ttggtaatc ccccgagtg gacgacttg tggtcattt 
      ctggacgca aacgagcag acttacatt gcttcactt 
      ctcgaagag gtgcacgca ggtttgcca attttaata 
      atagcaacg tatgaagaa gagttgcct ttgatgtta 
      cagaatttc ttttataat aacaccact gtggttatc 
      aacattgag aacccctca gaccacgaa gtagaggaa 
      tttttcatt cccctgttc tttggggac tcgccacac 
      agcttaagc acaatctta tcaggttgc aagcaaagc 
      tgtaaaaga aaaagtaac gaaactttg gaaacatta 
      aataagggg aaaggcccg cgatacgtc acttcttca 
      aaagtgtac aaattaaaa ccatcaggg aaaattaca 
      aagtctcgt tcaaacatc gcattaaaa aaaactttg 
      agactcagc cccgataaa gatctcatt cgttgtccc 
      tccaaacat ttcgacgaa ctccgggca gttaacacc 
      gaaagtttg tgcacttta tgcggaact acggaaaaa 
      aagaagcgt ctttctctg attctgacc gatttgttg 
      caacgaagt attcgatac ttttccaga cgcaggtca 
      ccacatgag cacaccagc agtcacaga agcatcagt 
      cttttgcaa aattacggc gattcttcc gatattgat 
      aagcagaaa atttacagt ttgtggcga cgcactgtc 
      atgaaaaca gccaaagac atgcctttg gctcatttg 
      gaagtcttg tacgacacc atttcggcc tgcattagc 
      attcacaaa gacgatttt caaaattta gttaaggtg 
      caatttttt caaaactag
    mRNA sequence
    • >TC033183 RA
      agttttaaa aaaaaataa ttttttcac tttcttgaa 
      gtaatgtac gtgtcaagt gtcatgccc tctgtattt 
      tgacttacc tttcaaaat taaatgtaa attccgcac 
      gtatttggc aaaaaaatt cgacgcgct gtcacaatg 
      tctagtatg aagcaacag aggtcatcc gaaaaattg 
      agtaggaaa tcaaaacaa acaaccgat ttttcaaaa 
      gttggtggt ttggcaaat cacatcaaa attttacgt 
      gatacgata atagtgccc ctattgcac aacaacgtt 
      tattcgcac tttaacatc aaaacaccc cgcggtatt 
      ctcttctac ggacctcca ggcacaggc aagactttg 
      gtcgctggc gctcttgca accgaactc aacaaagag 
      ggatttggc aaagtgagt ttctaccaa aggaaaggg 
      gccgatgtt ttggacaaa tgggtcggc gagtcggaa 
      aagaaactc cgaactcta tttgaaaac gcttcgaaa 
      aaccgacct tcgattata tttttcgac gagctggac 
      ggacttgct ccaattcgt tcggtcaaa aacgaccat 
      gtccactgc agcattgtc acaaccctt ttggccctt 
      atggacggg cttgacagc gtccctggg gtcatagtc 
      ataggtgca acaaaccga atcgagagc gtggatcca 
      gcgttgcga cgtcctggc cgttttgac cgggaattg 
      tacttccct ttgccctcc accgaggcc cggaaggaa 
      atcttgcaa gtgcacatg cagacgtgg agacaccgg 
      ccctccaat caatttatt tcagaattg gccgaaatg 
      accaccggc ttctgcggc tctgatttg caagcactt 
      tgcgctgag gctctacta tgtgccatg aagagacaa 
      cacccaaac atccaaaag tgcctcctg ggggcccgt 
      gtcaaaatc gaagccgga gaaattagg atagacgat 
      agtgatttt atcaacgct cgcaaaaac ttgattcct 
      tcgtcgcat aaaatgggg atcaggatg cgcaagttg 
      agtcctgtc atcaccccg ttgttgcaa aaacagtta 
      gatatgatc ttgtcaagg gttaacatg ttgtggccc 
      catttttcg atgcagttt tacaagtat ttgattggg 
      agtgagcgt tatgttgga agagttgtg ttggttggg 
      tcaaatttg caagggtta aatgcgcat ctagttccg 
      gctatttta caaaattac gaacactta ccggcgact 
      tttttggac gctgcgacc ctacaacac cgaagcctt 
      gtagcaaat ttttgtaaa aatttgccc tcgattttg 
      gtaatcccc cgagtggac gacttgtgg tcatttctg 
      gacgcaaac gagcagact tacattgct tcacttctc 
      gaagaggtg cacgcaggt ttgccaatt ttaataata 
      gcaacgtat gaagaagag ttgcctttg atgttacag 
      aatttcttt tataataac accactgtg gttatcaac 
      attgagaac ccctcagac cacgaagta gaggaattt 
      ttcattccc ctgttcttt ggggactcg ccacacagc 
      ttaagcaca atcttatca ggttgcaag caaagctgt 
      aaaagaaaa agtaacgaa actttggaa acattaaat 
      aaggggaaa ggcccgcga tacgtcact tcttcaaaa 
      gtgtacaaa ttaaaacca tcagggaaa attacaaag 
      tctcgttca aacatcgca ttaaaaaaa actttgaga 
      ctcagcccc gataaagat ctcattcgt tgtccctcc 
      aaacatttc gacgaactc cgggcagtt aacaccgaa 
      agtttgtgc actttatgc ggaactacg gaaaaaaag 
      aagcgtctt tctctgatt ctgaccgat ttgttgcaa 
      cgaagtatt cgatacttt tccagacgc aggtcacca 
      catgagcac accagcagt cacagaagc atcagtctt 
      ttgcaaaat tacggcgat tcttccgat attgataag 
      cagaaaatt tacagtttg tggcgacgc actgtcatg 
      aaaacagcc aaagacatg cctttggct catttggaa 
      gtcttgtac gacaccatt tcggcctgc attagcatt 
      cacaaagac gattttcaa aatttagtt aagaatttg 
      gatgacact cttctcgaa attgaggca actaagtag 
      aaatgtcgc tgtccagta gtttcccgt ggaggtcct 
      tgaggtaag tttaaaaat ttgtagtta tcaagattt 
      atgaatgga gaccatatg gttaagtca tgaattgaa 
      tttttgaca cgagctaaa ttgtggtga tgctgagag 
      tgcgatctt ttagattag tctttcgtg ggacagtgc 
      ttcaagcag acggcgtca attgttttg tccaagaat 
      tacgctgtt aagaaataa aaatatttt agttga
    • >TC033183 RB
      agttttaaa aaaaaataa ttttttcac tttcttgaa 
      gtaatgtac gtgtcaagt gtcatgccc tctgtattt 
      tgacttacc tttcaaaat taaatgtaa attccgcac 
      gtatttggc aaaaaaatt cgacgcgct gtcacaatg 
      tctagtatg aagcaacag aggtcatcc gaaaaattg 
      agtaggaaa tcaaaacaa acaaccgat ttttcaaaa 
      gttggtggt ttggcaaat cacatcaaa attttacgt 
      gatacgata atagtgccc ctattgcac aacaacgtt 
      tattcgcac tttaacatc aaaacaccc cgcggtatt 
      ctcttctac ggacctcca ggcacaggc aagactttg 
      gtcgctggc gctcttgca accgaactc aacaaagag 
      ggatttggc aaagtgagt ttctaccaa aggaaaggg 
      gccgatgtt ttggacaaa tgggtcggc gagtcggaa 
      aagaaactc cgaactcta tttgaaaac gcttcgaaa 
      aaccgacct tcgattata tttttcgac gagctggac 
      ggacttgct ccaattcgt tcggtcaaa aacgaccat 
      gtccactgc agcattgtc acaaccctt ttggccctt 
      atggacggg cttgacagc gtccctggg gtcatagtc 
      ataggtgca acaaaccga atcgagagc gtggatcca 
      gcgttgcga cgtcctggc cgttttgac cgggaattg 
      tacttccct ttgccctcc accgaggcc cggaaggaa 
      atcttgcaa gtgcacatg cagacgtgg agacaccgg 
      ccctccaat caatttatt tcagaattg gccgaaatg 
      accaccggc ttctgcggc tctgatttg caagcactt 
      tgcgctgag gctctacta tgtgccatg aagagacaa 
      cacccaaac atccaaaag tgcctcctg ggggcccgt 
      gtcaaaatc gaagccgga gaaattagg atagacgat 
      agtgatttt atcaacgct cgcaaaaac ttgattcct 
      tcgtcgcat aaaatgggg atcaggatg cgcaagttg 
      agtcctgtc atcaccccg ttgttgcaa aaacagtta 
      gatatgatc ttgtcaagg gttaacatg ttgtggccc 
      catttttcg atgcagttt tacaagtat ttgattggg 
      agtgagcgt tatgttgga agagttgtg ttggttggg 
      tcaaatttg caagggtta aatgcgcat ctagttccg 
      gctatttta caaaattac gaacactta ccggcgact 
      tttttggac gctgcgacc ctacaacac cgaagcctt 
      gtagcaaat ttttgtaaa aatttgccc tcgattttg 
      gtaatcccc cgagtggac gacttgtgg tcatttctg 
      gacgcaaac gagcagact tacattgct tcacttctc 
      gaagaggtg cacgcaggt ttgccaatt ttaataata 
      gcaacgtat gaagaagag ttgcctttg atgttacag 
      aatttcttt tataataac accactgtg gttatcaac 
      attgagaac ccctcagac cacgaagta gaggaattt 
      ttcattccc ctgttcttt ggggactcg ccacacagc 
      ttaagcaca atcttatca ggttgcaag caaagctgt 
      aaaagaaaa agtaacgaa actttggaa acattaaat 
      aaggggaaa ggcccgcga tacgtcact tcttcaaaa 
      gtgtacaaa ttaaaacca tcagggaaa attacaaag 
      tctcgttca aacatcgca ttaaaaaaa actttgaga 
      ctcagcccc gataaagat ctcattcgt tgtccctcc 
      aaacatttc gacgaactc cgggcagtt aacaccgaa 
      agtttgtgc actttatgc ggaactacg gaaaaaaag 
      aagcgtctt tctctgatt ctgaccgat ttgttgcaa 
      cgaagtatt cgatacttt tccagacgc aggtcacca 
      catgagcac accagcagt cacagaagc atcagtctt 
      ttgcaaaat tacggcgat tcttccgat attgataag 
      cagaaaatt tacagtttg tggcgacgc actgtcatg 
      aaaacagcc aaagacatg cctttggct catttggaa 
      gtcttgtac gacaccatt tcggcctgc attagcatt 
      cacaaagac gattttcaa aatttagtt aaggtgcaa 
      ttttttcaa aactagtta cttattata cagggtgct 
      agggaatgg cttagcaat atttcgtat gt