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Gene information
General information
TC014488 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 3225919..3234333
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014488
    iBeetle screen iB_02802
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment random number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014488 RA
      MDFETLASG LNCTPPAID DFPRDFFTE KERQDGAVV 
      VHVVVSLYL FVALAVVCD KFFVPAVEK ICHALNMSA 
      DVAGATFMA AATSAPELF VNVIGTFIT EGDIGVGTI 
      VGSAVFNIL AVAACCGIG AGLCGVKVV PLDWWPLTR 
      DCLAYGITV SILICIIHD ERVEWYEAL TLVLLYTVY 
      ILIMYFDKS IQRCARGEY HTLPLQSPS KLVLHENAP 
      LLGGFKSAQ PPHDSTPQS IEAAEAHLP LQPNKEIKD 
      ISDNHNTEN VVVVPSHLN GSLKVPEAE TEEAKKTEP 
      TNMVEDEPH SLWKWPAGR SKFTQFTWV ITWPIHLIF 
      LFTIPDCEK PRFKKWFPL TFIMCIIWI GSLSYVVAW 
      MITIIGDTL KIPDSVMGI TFLAAGTSI PEAVSSVIV 
      AKQGHGSMG ISNSIGSNT FDILLCLGL PWFIKATFM 
      PTIAGKHWV GINSAGIEY SAISLLSSL LLLYLVFAV 
      NKFRLDKRV GRICLLMYA IFLILASLI ELNAFFRVN 
      LPTCGR
    • >TC014488 RB
      MGLNCTPPA IDDFPRDFF TEKERQDGA VVVHVVVSL 
      YLFVALAVV CDKFFVPAV EKICHALNM SADVAGATF 
      MAAATSAPE LFVNVIGTF ITEGDIGVG TIVGSAVFN 
      ILAVAACCG IGAGLCGVK VVPLDWWPL TRDCLAYGI 
      TVSILICII HDERVEWYE ALTLVLLYT VYILIMYFD 
      KSIQRCARG EYHTLPLQS PSKLVLHEN APLLGGFKS 
      AQPPHDSTP QSIEAAEAH LPLQPNKEI KDISDNHNT 
      ENVVVVPSH LNGSLKVPE AETEEAKKT EPTNMVEDE 
      PHSLWKWPA GRSKFTQFT WVITWPIHL IFLFTIPDC 
      EKPRFKKWF PLTFIMCII WIGSLSYVV AWMITIIGD 
      TLKIPDSVM GITFLAAGT SIPEAVSSV IVAKQGHGS 
      MGISNSIGS NTFDILLCL GLPWFIKAT FMPTIAGKH 
      WVGINSAGI EYSAISLLS SLLLLYLVF AVNKFRLDK 
      RVGRICLLM YAIFLILAS LIELNAFFR VNLPTCGR
    CDS sequence
    • >TC014488 RA
      atggacttc gaaacgttg gcatcagga cttaattgt 
      actccaccc gccatcgat gattttcct cgcgatttt 
      ttcaccgaa aaggaaaga caagatggg gccgtggtt 
      gtacatgtc gtcgtatcc ctctacctc ttcgtcgcc 
      ttggccgta gtctgtgat aaatttttt gtaccggca 
      gtagaaaaa atatgccat gctctaaat atgtcagcg 
      gacgtcgca ggagcgacg tttatggca gctgcgact 
      tctgctcct gagcttttc gtcaacgtc atcggtacc 
      ttcatcacc gagggcgac attggcgtt ggtaccatt 
      gtcggatcg gccgttttt aacattctg gccgtcgcg 
      gcgtgctgt ggcatcggc gcaggattg tgtggcgta 
      aaagtggtt cctttggac tggtggccc ctgaccagg 
      gactgtcta gcttatggc atcacagtt tccatcctc 
      atctgtata attcatgac gaaagggtg gaatggtac 
      gaagcttta accctggta ctgctctac acagtctac 
      atccttata atgtatttc gacaagtcc attcaaagg 
      tgtgccaga ggtgagtac cacacttta cccttgcaa 
      tctccttca aagttagtt ctccacgaa aatgcacca 
      cttttaggt ggtttcaaa tccgcacag cccccgcac 
      gactccacc ccccagtct atagaggcg gctgaggcc 
      cacttaccg ctccaaccc aacaaagaa attaaagac 
      atcagcgat aatcacaac acggagaac gtggtagtg 
      gtaccatcg cacctcaac ggttccctc aaagtaccg 
      gaagcggaa accgaagaa gcgaaaaag accgaaccc 
      acgaatatg gtcgaagac gagccgcac agtttgtgg 
      aaatggccg gctggacga agcaaattc acccaattc 
      acatgggtc atcacctgg cccatccat ctaatattc 
      ctgttcaca attcccgac tgcgagaaa cccagattt 
      aagaaatgg ttccctttg accttcatc atgtgtatt 
      atctggatc gggtccttg tcgtacgtt gtggcttgg 
      atgattaca attatcggg gacactcta aaaatccca 
      gattccgtc atgggaatc acttttttg gccgccgga 
      accagcatt cccgaggcc gtttcgagc gtaattgtg 
      gccaagcag ggtcacggc tcgatgggc ataagcaac 
      tcgatcggg tccaacacc ttcgacatt ttgctttgt 
      ttgggcctt ccttggttc atcaaagcc acgtttatg 
      ccgacaatt gcaggaaaa cattgggtg gggataaat 
      tcggcggga attgagtac agcgccatc tcccttttg 
      tcgtcgctg ctcttgcta tacctggtg tttgccgta 
      aataaattc cgactagat aagagagtg ggtcggata 
      tgtttgttg atgtacgcc atttttctt atcttggcc 
      agtctcatc gagcttaat gccttcttc agagtcaat 
      ttaccgact tgtgggcgg tga
    • >TC014488 RB
      atgggactt aattgtact ccacccgcc atcgatgat 
      tttcctcgc gattttttc accgaaaag gaaagacaa 
      gatggggcc gtggttgta catgtcgtc gtatccctc 
      tacctcttc gtcgccttg gccgtagtc tgtgataaa 
      ttttttgta ccggcagta gaaaaaata tgccatgct 
      ctaaatatg tcagcggac gtcgcagga gcgacgttt 
      atggcagct gcgacttct gctcctgag cttttcgtc 
      aacgtcatc ggtaccttc atcaccgag ggcgacatt 
      ggcgttggt accattgtc ggatcggcc gtttttaac 
      attctggcc gtcgcggcg tgctgtggc atcggcgca 
      ggattgtgt ggcgtaaaa gtggttcct ttggactgg 
      tggcccctg accagggac tgtctagct tatggcatc 
      acagtttcc atcctcatc tgtataatt catgacgaa 
      agggtggaa tggtacgaa gctttaacc ctggtactg 
      ctctacaca gtctacatc cttataatg tatttcgac 
      aagtccatt caaaggtgt gccagaggt gagtaccac 
      actttaccc ttgcaatct ccttcaaag ttagttctc 
      cacgaaaat gcaccactt ttaggtggt ttcaaatcc 
      gcacagccc ccgcacgac tccaccccc cagtctata 
      gaggcggct gaggcccac ttaccgctc caacccaac 
      aaagaaatt aaagacatc agcgataat cacaacacg 
      gagaacgtg gtagtggta ccatcgcac ctcaacggt 
      tccctcaaa gtaccggaa gcggaaacc gaagaagcg 
      aaaaagacc gaacccacg aatatggtc gaagacgag 
      ccgcacagt ttgtggaaa tggccggct ggacgaagc 
      aaattcacc caattcaca tgggtcatc acctggccc 
      atccatcta atattcctg ttcacaatt cccgactgc 
      gagaaaccc agatttaag aaatggttc cctttgacc 
      ttcatcatg tgtattatc tggatcggg tccttgtcg 
      tacgttgtg gcttggatg attacaatt atcggggac 
      actctaaaa atcccagat tccgtcatg ggaatcact 
      tttttggcc gccggaacc agcattccc gaggccgtt 
      tcgagcgta attgtggcc aagcagggt cacggctcg 
      atgggcata agcaactcg atcgggtcc aacaccttc 
      gacattttg ctttgtttg ggccttcct tggttcatc 
      aaagccacg tttatgccg acaattgca ggaaaacat 
      tgggtgggg ataaattcg gcgggaatt gagtacagc 
      gccatctcc cttttgtcg tcgctgctc ttgctatac 
      ctggtgttt gccgtaaat aaattccga ctagataag 
      agagtgggt cggatatgt ttgttgatg tacgccatt 
      tttcttatc ttggccagt ctcatcgag cttaatgcc 
      ttcttcaga gtcaattta ccgacttgt gggcggtga
    mRNA sequence
    • >TC014488 RA
      ggtcggttg tttacgatg taatcggag catcgacaa 
      taagccagt tggagtaca atacaacaa cagcagtga 
      cattagcaa gtgcccaat attagagtc cagtcgagc 
      agatgattg tcaccagca gtagtgtaa taagagggg 
      cgggggtca aatcagtga gtgaagagt cgcagcagg 
      aacaacatg gacttcgaa acgttggca tcaggactt 
      aattgtact ccacccgcc atcgatgat tttcctcgc 
      gattttttc accgaaaag gaaagacaa gatggggcc 
      gtggttgta catgtcgtc gtatccctc tacctcttc 
      gtcgccttg gccgtagtc tgtgataaa ttttttgta 
      ccggcagta gaaaaaata tgccatgct ctaaatatg 
      tcagcggac gtcgcagga gcgacgttt atggcagct 
      gcgacttct gctcctgag cttttcgtc aacgtcatc 
      ggtaccttc atcaccgag ggcgacatt ggcgttggt 
      accattgtc ggatcggcc gtttttaac attctggcc 
      gtcgcggcg tgctgtggc atcggcgca ggattgtgt 
      ggcgtaaaa gtggttcct ttggactgg tggcccctg 
      accagggac tgtctagct tatggcatc acagtttcc 
      atcctcatc tgtataatt catgacgaa agggtggaa 
      tggtacgaa gctttaacc ctggtactg ctctacaca 
      gtctacatc cttataatg tatttcgac aagtccatt 
      caaaggtgt gccagaggt gagtaccac actttaccc 
      ttgcaatct ccttcaaag ttagttctc cacgaaaat 
      gcaccactt ttaggtggt ttcaaatcc gcacagccc 
      ccgcacgac tccaccccc cagtctata gaggcggct 
      gaggcccac ttaccgctc caacccaac aaagaaatt 
      aaagacatc agcgataat cacaacacg gagaacgtg 
      gtagtggta ccatcgcac ctcaacggt tccctcaaa 
      gtaccggaa gcggaaacc gaagaagcg aaaaagacc 
      gaacccacg aatatggtc gaagacgag ccgcacagt 
      ttgtggaaa tggccggct ggacgaagc aaattcacc 
      caattcaca tgggtcatc acctggccc atccatcta 
      atattcctg ttcacaatt cccgactgc gagaaaccc 
      agatttaag aaatggttc cctttgacc ttcatcatg 
      tgtattatc tggatcggg tccttgtcg tacgttgtg 
      gcttggatg attacaatt atcggggac actctaaaa 
      atcccagat tccgtcatg ggaatcact tttttggcc 
      gccggaacc agcattccc gaggccgtt tcgagcgta 
      attgtggcc aagcagggt cacggctcg atgggcata 
      agcaactcg atcgggtcc aacaccttc gacattttg 
      ctttgtttg ggccttcct tggttcatc aaagccacg 
      tttatgccg acaattgca ggaaaacat tgggtgggg 
      ataaattcg gcgggaatt gagtacagc gccatctcc 
      cttttgtcg tcgctgctc ttgctatac ctggtgttt 
      gccgtaaat aaattccga ctagataag agagtgggt 
      cggatatgt ttgttgatg tacgccatt tttcttatc 
      ttggccagt ctcatcgag cttaatgcc ttcttcaga 
      gtcaattta ccgacttgt gggcggtga agttgatca 
      gcgctcttc ttgttttca tagatttta ttttattac 
      agttttaat gggaaaatt gtgatttct ttctgcaat 
      attcttggt tcaccatca tgtcagatc tcaaacaat 
      tctgttgta gagtaagga aggaaattt gcatcgcac 
      ctcattcta gtttaagtt attgcgtaa tctgtcctg 
      tacatagaa caatttaaa ccatcctaa caacagtag 
      ggttatttt ttaaaaact attttattt tgtagctga 
      cattatgta cattatgtt gcattacag acagtccta 
      ctattaatt ttgtaat
    • >TC014488 RB
      ggtcggttg tttacgatg taatcggag catcgacaa 
      taagccagt tggagtaca atacaacaa cagcagtga 
      cattagcaa gtgcccaat attagagtc cagtcgagc 
      agatgattg tcaccagca gtagtgtaa taagagggg 
      cgggggtca aatcagtga gtgaagagt cgcagcagg 
      aacaacatg gacttcgaa acgttggca tcagtccaa 
      ctagccacc agtgaccca aaaccttcc ttacaacac 
      tcgctgaaa aaaacgcga ctttggcgc gattttccc 
      aacaatttc ctttcactt tggcccaaa tcgcttcgc 
      aaatgcagt ttgcattga ttttgcggt acaaagagt 
      gtacgtcgc gacttttcc acttttcat cgagtcttg 
      aacgatccg tggattcgt cctgtttag tgtaattgg 
      gtctctaat gccatcagt ttgcctgtc ttcgaaatg 
      ggacttaat tgtactcca cccgccatc gatgatttt 
      cctcgcgat tttttcacc gaaaaggaa agacaagat 
      ggggccgtg gttgtacat gtcgtcgta tccctctac 
      ctcttcgtc gccttggcc gtagtctgt gataaattt 
      tttgtaccg gcagtagaa aaaatatgc catgctcta 
      aatatgtca gcggacgtc gcaggagcg acgtttatg 
      gcagctgcg acttctgct cctgagctt ttcgtcaac 
      gtcatcggt accttcatc accgagggc gacattggc 
      gttggtacc attgtcgga tcggccgtt tttaacatt 
      ctggccgtc gcggcgtgc tgtggcatc ggcgcagga 
      ttgtgtggc gtaaaagtg gttcctttg gactggtgg 
      cccctgacc agggactgt ctagcttat ggcatcaca 
      gtttccatc ctcatctgt ataattcat gacgaaagg 
      gtggaatgg tacgaagct ttaaccctg gtactgctc 
      tacacagtc tacatcctt ataatgtat ttcgacaag 
      tccattcaa aggtgtgcc agaggtgag taccacact 
      ttacccttg caatctcct tcaaagtta gttctccac 
      gaaaatgca ccactttta ggtggtttc aaatccgca 
      cagcccccg cacgactcc accccccag tctatagag 
      gcggctgag gcccactta ccgctccaa cccaacaaa 
      gaaattaaa gacatcagc gataatcac aacacggag 
      aacgtggta gtggtacca tcgcacctc aacggttcc 
      ctcaaagta ccggaagcg gaaaccgaa gaagcgaaa 
      aagaccgaa cccacgaat atggtcgaa gacgagccg 
      cacagtttg tggaaatgg ccggctgga cgaagcaaa 
      ttcacccaa ttcacatgg gtcatcacc tggcccatc 
      catctaata ttcctgttc acaattccc gactgcgag 
      aaacccaga tttaagaaa tggttccct ttgaccttc 
      atcatgtgt attatctgg atcgggtcc ttgtcgtac 
      gttgtggct tggatgatt acaattatc ggggacact 
      ctaaaaatc ccagattcc gtcatggga atcactttt 
      ttggccgcc ggaaccagc attcccgag gccgtttcg 
      agcgtaatt gtggccaag cagggtcac ggctcgatg 
      ggcataagc aactcgatc gggtccaac accttcgac 
      attttgctt tgtttgggc cttccttgg ttcatcaaa 
      gccacgttt atgccgaca attgcagga aaacattgg 
      gtggggata aattcggcg ggaattgag tacagcgcc 
      atctccctt ttgtcgtcg ctgctcttg ctatacctg 
      gtgtttgcc gtaaataaa ttccgacta gataagaga 
      gtgggtcgg atatgtttg ttgatgtac gccattttt 
      cttatcttg gccagtctc atcgagctt aatgccttc 
      ttcagagtc aatttaccg acttgtggg cggtgaagt 
      tgatcagcg ctcttcttg ttttcatag attttattt 
      tattacagt tttaatggg aaaattgtg atttctttc 
      tgcaatatt cttggttca ccatcatgt cagatctca 
      aacaattct gttgtagag taaggaagg aaatttgca 
      tcgcacctc attctagtt taagttatt gcgtaatct 
      gtcctgtac atagaacaa tttaaacca tcctaacaa 
      cagtagggt tatttttta aaaactatt ttattttgt 
      agctgacat tatgtacat tatgttgca ttacagaca 
      gtcctacta ttaattttg taat