Status
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Gene information
General information
TC014488 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11141333..11149747
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014488
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02802

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment random number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014488 RA
      MDFETLASG LNCTPPAID DFPRDFFTE KERQDGAVV 
VHVVVSLYL FVALAVVCD KFFVPAVEK ICHALNMSA 
DVAGATFMA AATSAPELF VNVIGTFIT EGDIGVGTI 
VGSAVFNIL AVAACCGIG AGLCGVKVV PLDWWPLTR 
DCLAYGITV SILICIIHD ERVEWYEAL TLVLLYTVY 
ILIMYFDKS IQRCARGEY HTLPLQSPS KLVLHENAP 
LLGGFKSAQ PPHDSTPQS IEAAEAHLP LQPNKEIKD 
ISDNHNTEN VVVVPSHLN GSLKVPEAE TEEAKKTEP 
TNMVEDEPH SLWKWPAGR SKFTQFTWV ITWPIHLIF 
LFTIPDCEK PRFKKWFPL TFIMCIIWI GSLSYVVAW 
MITIIGDTL KIPDSVMGI TFLAAGTSI PEAVSSVIV 
AKQGHGSMG ISNSIGSNT FDILLCLGL PWFIKATFM 
PTIAGKHWV GINSAGIEY SAISLLSSL LLLYLVFAV 
NKFRLDKRV GRICLLMYA IFLILASLI ELNAFFRVN 
LPTCGR
    • >TC014488 RB
      MGLNCTPPA IDDFPRDFF TEKERQDGA VVVHVVVSL 
YLFVALAVV CDKFFVPAV EKICHALNM SADVAGATF 
MAAATSAPE LFVNVIGTF ITEGDIGVG TIVGSAVFN 
ILAVAACCG IGAGLCGVK VVPLDWWPL TRDCLAYGI 
TVSILICII HDERVEWYE ALTLVLLYT VYILIMYFD 
KSIQRCARG EYHTLPLQS PSKLVLHEN APLLGGFKS 
AQPPHDSTP QSIEAAEAH LPLQPNKEI KDISDNHNT 
ENVVVVPSH LNGSLKVPE AETEEAKKT EPTNMVEDE 
PHSLWKWPA GRSKFTQFT WVITWPIHL IFLFTIPDC 
EKPRFKKWF PLTFIMCII WIGSLSYVV AWMITIIGD 
TLKIPDSVM GITFLAAGT SIPEAVSSV IVAKQGHGS 
MGISNSIGS NTFDILLCL GLPWFIKAT FMPTIAGKH 
WVGINSAGI EYSAISLLS SLLLLYLVF AVNKFRLDK 
RVGRICLLM YAIFLILAS LIELNAFFR VNLPTCGR
    CDS sequence
    • >TC014488 RA
      atggacttc gaaacgttg gcatcagga cttaattgt 
actccaccc gccatcgat gattttcct cgcgatttt 
ttcaccgaa aaggaaaga caagatggg gccgtggtt 
gtacatgtc gtcgtatcc ctctacctc ttcgtcgcc 
ttggccgta gtctgtgat aaatttttt gtaccggca 
gtagaaaaa atatgccat gctctaaat atgtcagcg 
gacgtcgca ggagcgacg tttatggca gctgcgact 
tctgctcct gagcttttc gtcaacgtc atcggtacc 
ttcatcacc gagggcgac attggcgtt ggtaccatt 
gtcggatcg gccgttttt aacattctg gccgtcgcg 
gcgtgctgt ggcatcggc gcaggattg tgtggcgta 
aaagtggtt cctttggac tggtggccc ctgaccagg 
gactgtcta gcttatggc atcacagtt tccatcctc 
atctgtata attcatgac gaaagggtg gaatggtac 
gaagcttta accctggta ctgctctac acagtctac 
atccttata atgtatttc gacaagtcc attcaaagg 
tgtgccaga ggtgagtac cacacttta cccttgcaa 
tctccttca aagttagtt ctccacgaa aatgcacca 
cttttaggt ggtttcaaa tccgcacag cccccgcac 
gactccacc ccccagtct atagaggcg gctgaggcc 
cacttaccg ctccaaccc aacaaagaa attaaagac 
atcagcgat aatcacaac acggagaac gtggtagtg 
gtaccatcg cacctcaac ggttccctc aaagtaccg 
gaagcggaa accgaagaa gcgaaaaag accgaaccc 
acgaatatg gtcgaagac gagccgcac agtttgtgg 
aaatggccg gctggacga agcaaattc acccaattc 
acatgggtc atcacctgg cccatccat ctaatattc 
ctgttcaca attcccgac tgcgagaaa cccagattt 
aagaaatgg ttccctttg accttcatc atgtgtatt 
atctggatc gggtccttg tcgtacgtt gtggcttgg 
atgattaca attatcggg gacactcta aaaatccca 
gattccgtc atgggaatc acttttttg gccgccgga 
accagcatt cccgaggcc gtttcgagc gtaattgtg 
gccaagcag ggtcacggc tcgatgggc ataagcaac 
tcgatcggg tccaacacc ttcgacatt ttgctttgt 
ttgggcctt ccttggttc atcaaagcc acgtttatg 
ccgacaatt gcaggaaaa cattgggtg gggataaat 
tcggcggga attgagtac agcgccatc tcccttttg 
tcgtcgctg ctcttgcta tacctggtg tttgccgta 
aataaattc cgactagat aagagagtg ggtcggata 
tgtttgttg atgtacgcc atttttctt atcttggcc 
agtctcatc gagcttaat gccttcttc agagtcaat 
ttaccgact tgtgggcgg tga
    • >TC014488 RB
      atgggactt aattgtact ccacccgcc atcgatgat 
tttcctcgc gattttttc accgaaaag gaaagacaa 
gatggggcc gtggttgta catgtcgtc gtatccctc 
tacctcttc gtcgccttg gccgtagtc tgtgataaa 
ttttttgta ccggcagta gaaaaaata tgccatgct 
ctaaatatg tcagcggac gtcgcagga gcgacgttt 
atggcagct gcgacttct gctcctgag cttttcgtc 
aacgtcatc ggtaccttc atcaccgag ggcgacatt 
ggcgttggt accattgtc ggatcggcc gtttttaac 
attctggcc gtcgcggcg tgctgtggc atcggcgca 
ggattgtgt ggcgtaaaa gtggttcct ttggactgg 
tggcccctg accagggac tgtctagct tatggcatc 
acagtttcc atcctcatc tgtataatt catgacgaa 
agggtggaa tggtacgaa gctttaacc ctggtactg 
ctctacaca gtctacatc cttataatg tatttcgac 
aagtccatt caaaggtgt gccagaggt gagtaccac 
actttaccc ttgcaatct ccttcaaag ttagttctc 
cacgaaaat gcaccactt ttaggtggt ttcaaatcc 
gcacagccc ccgcacgac tccaccccc cagtctata 
gaggcggct gaggcccac ttaccgctc caacccaac 
aaagaaatt aaagacatc agcgataat cacaacacg 
gagaacgtg gtagtggta ccatcgcac ctcaacggt 
tccctcaaa gtaccggaa gcggaaacc gaagaagcg 
aaaaagacc gaacccacg aatatggtc gaagacgag 
ccgcacagt ttgtggaaa tggccggct ggacgaagc 
aaattcacc caattcaca tgggtcatc acctggccc 
atccatcta atattcctg ttcacaatt cccgactgc 
gagaaaccc agatttaag aaatggttc cctttgacc 
ttcatcatg tgtattatc tggatcggg tccttgtcg 
tacgttgtg gcttggatg attacaatt atcggggac 
actctaaaa atcccagat tccgtcatg ggaatcact 
tttttggcc gccggaacc agcattccc gaggccgtt 
tcgagcgta attgtggcc aagcagggt cacggctcg 
atgggcata agcaactcg atcgggtcc aacaccttc 
gacattttg ctttgtttg ggccttcct tggttcatc 
aaagccacg tttatgccg acaattgca ggaaaacat 
tgggtgggg ataaattcg gcgggaatt gagtacagc 
gccatctcc cttttgtcg tcgctgctc ttgctatac 
ctggtgttt gccgtaaat aaattccga ctagataag 
agagtgggt cggatatgt ttgttgatg tacgccatt 
tttcttatc ttggccagt ctcatcgag cttaatgcc 
ttcttcaga gtcaattta ccgacttgt gggcggtga
    mRNA sequence
    • >TC014488 RA
      ggtcggttg tttacgatg taatcggag catcgacaa 
taagccagt tggagtaca atacaacaa cagcagtga 
cattagcaa gtgcccaat attagagtc cagtcgagc 
agatgattg tcaccagca gtagtgtaa taagagggg 
cgggggtca aatcagtga gtgaagagt cgcagcagg 
aacaacatg gacttcgaa acgttggca tcaggactt 
aattgtact ccacccgcc atcgatgat tttcctcgc 
gattttttc accgaaaag gaaagacaa gatggggcc 
gtggttgta catgtcgtc gtatccctc tacctcttc 
gtcgccttg gccgtagtc tgtgataaa ttttttgta 
ccggcagta gaaaaaata tgccatgct ctaaatatg 
tcagcggac gtcgcagga gcgacgttt atggcagct 
gcgacttct gctcctgag cttttcgtc aacgtcatc 
ggtaccttc atcaccgag ggcgacatt ggcgttggt 
accattgtc ggatcggcc gtttttaac attctggcc 
gtcgcggcg tgctgtggc atcggcgca ggattgtgt 
ggcgtaaaa gtggttcct ttggactgg tggcccctg 
accagggac tgtctagct tatggcatc acagtttcc 
atcctcatc tgtataatt catgacgaa agggtggaa 
tggtacgaa gctttaacc ctggtactg ctctacaca 
gtctacatc cttataatg tatttcgac aagtccatt 
caaaggtgt gccagaggt gagtaccac actttaccc 
ttgcaatct ccttcaaag ttagttctc cacgaaaat 
gcaccactt ttaggtggt ttcaaatcc gcacagccc 
ccgcacgac tccaccccc cagtctata gaggcggct 
gaggcccac ttaccgctc caacccaac aaagaaatt 
aaagacatc agcgataat cacaacacg gagaacgtg 
gtagtggta ccatcgcac ctcaacggt tccctcaaa 
gtaccggaa gcggaaacc gaagaagcg aaaaagacc 
gaacccacg aatatggtc gaagacgag ccgcacagt 
ttgtggaaa tggccggct ggacgaagc aaattcacc 
caattcaca tgggtcatc acctggccc atccatcta 
atattcctg ttcacaatt cccgactgc gagaaaccc 
agatttaag aaatggttc cctttgacc ttcatcatg 
tgtattatc tggatcggg tccttgtcg tacgttgtg 
gcttggatg attacaatt atcggggac actctaaaa 
atcccagat tccgtcatg ggaatcact tttttggcc 
gccggaacc agcattccc gaggccgtt tcgagcgta 
attgtggcc aagcagggt cacggctcg atgggcata 
agcaactcg atcgggtcc aacaccttc gacattttg 
ctttgtttg ggccttcct tggttcatc aaagccacg 
tttatgccg acaattgca ggaaaacat tgggtgggg 
ataaattcg gcgggaatt gagtacagc gccatctcc 
cttttgtcg tcgctgctc ttgctatac ctggtgttt 
gccgtaaat aaattccga ctagataag agagtgggt 
cggatatgt ttgttgatg tacgccatt tttcttatc 
ttggccagt ctcatcgag cttaatgcc ttcttcaga 
gtcaattta ccgacttgt gggcggtga agttgatca 
gcgctcttc ttgttttca tagatttta ttttattac 
agttttaat gggaaaatt gtgatttct ttctgcaat 
attcttggt tcaccatca tgtcagatc tcaaacaat 
tctgttgta gagtaagga aggaaattt gcatcgcac 
ctcattcta gtttaagtt attgcgtaa tctgtcctg 
tacatagaa caatttaaa ccatcctaa caacagtag 
ggttatttt ttaaaaact attttattt tgtagctga 
cattatgta cattatgtt gcattacag acagtccta 
ctattaatt ttgtaat
    • >TC014488 RB
      ggtcggttg tttacgatg taatcggag catcgacaa 
taagccagt tggagtaca atacaacaa cagcagtga 
cattagcaa gtgcccaat attagagtc cagtcgagc 
agatgattg tcaccagca gtagtgtaa taagagggg 
cgggggtca aatcagtga gtgaagagt cgcagcagg 
aacaacatg gacttcgaa acgttggca tcagtccaa 
ctagccacc agtgaccca aaaccttcc ttacaacac 
tcgctgaaa aaaacgcga ctttggcgc gattttccc 
aacaatttc ctttcactt tggcccaaa tcgcttcgc 
aaatgcagt ttgcattga ttttgcggt acaaagagt 
gtacgtcgc gacttttcc acttttcat cgagtcttg 
aacgatccg tggattcgt cctgtttag tgtaattgg 
gtctctaat gccatcagt ttgcctgtc ttcgaaatg 
ggacttaat tgtactcca cccgccatc gatgatttt 
cctcgcgat tttttcacc gaaaaggaa agacaagat 
ggggccgtg gttgtacat gtcgtcgta tccctctac 
ctcttcgtc gccttggcc gtagtctgt gataaattt 
tttgtaccg gcagtagaa aaaatatgc catgctcta 
aatatgtca gcggacgtc gcaggagcg acgtttatg 
gcagctgcg acttctgct cctgagctt ttcgtcaac 
gtcatcggt accttcatc accgagggc gacattggc 
gttggtacc attgtcgga tcggccgtt tttaacatt 
ctggccgtc gcggcgtgc tgtggcatc ggcgcagga 
ttgtgtggc gtaaaagtg gttcctttg gactggtgg 
cccctgacc agggactgt ctagcttat ggcatcaca 
gtttccatc ctcatctgt ataattcat gacgaaagg 
gtggaatgg tacgaagct ttaaccctg gtactgctc 
tacacagtc tacatcctt ataatgtat ttcgacaag 
tccattcaa aggtgtgcc agaggtgag taccacact 
ttacccttg caatctcct tcaaagtta gttctccac 
gaaaatgca ccactttta ggtggtttc aaatccgca 
cagcccccg cacgactcc accccccag tctatagag 
gcggctgag gcccactta ccgctccaa cccaacaaa 
gaaattaaa gacatcagc gataatcac aacacggag 
aacgtggta gtggtacca tcgcacctc aacggttcc 
ctcaaagta ccggaagcg gaaaccgaa gaagcgaaa 
aagaccgaa cccacgaat atggtcgaa gacgagccg 
cacagtttg tggaaatgg ccggctgga cgaagcaaa 
ttcacccaa ttcacatgg gtcatcacc tggcccatc 
catctaata ttcctgttc acaattccc gactgcgag 
aaacccaga tttaagaaa tggttccct ttgaccttc 
atcatgtgt attatctgg atcgggtcc ttgtcgtac 
gttgtggct tggatgatt acaattatc ggggacact 
ctaaaaatc ccagattcc gtcatggga atcactttt 
ttggccgcc ggaaccagc attcccgag gccgtttcg 
agcgtaatt gtggccaag cagggtcac ggctcgatg 
ggcataagc aactcgatc gggtccaac accttcgac 
attttgctt tgtttgggc cttccttgg ttcatcaaa 
gccacgttt atgccgaca attgcagga aaacattgg 
gtggggata aattcggcg ggaattgag tacagcgcc 
atctccctt ttgtcgtcg ctgctcttg ctatacctg 
gtgtttgcc gtaaataaa ttccgacta gataagaga 
gtgggtcgg atatgtttg ttgatgtac gccattttt 
cttatcttg gccagtctc atcgagctt aatgccttc 
ttcagagtc aatttaccg acttgtggg cggtgaagt 
tgatcagcg ctcttcttg ttttcatag attttattt 
tattacagt tttaatggg aaaattgtg atttctttc 
tgcaatatt cttggttca ccatcatgt cagatctca 
aacaattct gttgtagag taaggaagg aaatttgca 
tcgcacctc attctagtt taagttatt gcgtaatct 
gtcctgtac atagaacaa tttaaacca tcctaacaa 
cagtagggt tatttttta aaaactatt ttattttgt 
agctgacat tatgtacat tatgttgca ttacagaca 
gtcctacta ttaattttg taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY