Status
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Gene information
General information
TC013350 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10672755..10675227
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013350
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02801

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013350 RA
      MYKGDCKWH HDYVRLAFD TGFKFEKHK SDNFDRNKI 
      SLIDIDRVI EERDFATVE TLIPGITKF VLDPDLAQV 
      LDVSFVKTF KLSQLAIQY LLFCKKYLD NTVIVLKDE 
      LKKVRGENK ELNLFVDDL KEHIATLMR EIDEKVAFK 
      CEQCLKVFS SEEFLNSHI RRRHSTPTQ VPETDKLQM 
      EIKELKGRL NDAEKMIQK EHQEIDSNI SRETDFKKI 
      EDLQQKFED LRLQVQSEL KVLQTQHNF QEKYEKWFE 
      KMALQGGSL GRRRESMTQ TEEGASSRV VMYQEKNKE 
      NVPELSNAQ KQITQFEEA LETKVASSL QKIEDQMQS 
      FWNKLNEME MYRCRDMED KPKPSAKPR SKYSDKHLI 
      ETRQRNESS QQRDKKQAK SVSLEEDNE ITDASPQSQ 
      ETPKASPIK VMRAQPQIK VPKVKTEKI ESGINLTEI 
      DDDTTTSET LASEEKISD SPQEKVIKP PLKPQPVPR 
      KRPKTSKDI VSSNIIDDE LLQHFKLEI ENILKARLQ 
      DMGVSPDWK GLPNRSFQR ALEIVNHQA NLVKKTCPN 
      FDSIASQIE KDVAKKIDN KRQPKKKPK KLAVQIEIK 
      NRQMYDTDT ESEVKPVKV KEKQFSAPY SAVIAELKT 
      VTSKIDASD KSEESIKDE KRVKEDQTK GVLKSYPSV 
      GSLTKKKVL FNLDTEEKK ELPAKDGGS TTSITSSVF 
      DGTPRNEEP KKKGKDIED LSDFDFSDM
    CDS sequence
    • >TC013350 RA
      atgtacaag ggagattgc aagtggcac catgattac 
      gtacgtcta gcttttgac accggcttt aaattcgaa 
      aaacacaaa tcggacaac tttgacaga aacaagata 
      agtttaatt gatattgat cgagtgatt gaagagaga 
      gactttgca accgttgaa acccttatt cctgggata 
      acaaaattt gttctagac ccggacctg gcgcaggtc 
      ttggacgtt agtttcgta aaaacgttc aaattgagc 
      caattggcg attcagtat ttactattt tgcaaaaaa 
      tatctcgat aataccgta atagtgttg aaagatgag 
      ttgaagaaa gttcgaggg gaaaacaaa gaattgaat 
      ttatttgtt gacgatctc aaggagcac attgcgact 
      ttgatgcgc gaaattgac gaaaaagtt gccttcaaa 
      tgcgaacag tgccttaaa gttttctca agtgaggaa 
      tttttaaat tcgcatatc aggcgccgc catagtacc 
      cccacacag gtccccgaa acggacaaa ctccagatg 
      gaaatcaag gaattgaaa gggcgcctc aacgatgcg 
      gagaaaatg attcaaaag gagcaccag gagattgac 
      tcaaatatc agtagggag acggatttt aagaaaatt 
      gaagatttg cagcaaaag tttgaggat ttgcggctg 
      caggtccag tccgaattg aaggttttg cagacgcag 
      cataacttc caggagaag tatgaaaag tggtttgag 
      aaaatggcc cttcagggg ggcagtttg gggcgcagg 
      agggagagc atgacgcag actgaggag ggggcgtcg 
      agtcgtgtt gttatgtat caggaaaaa aataaagaa 
      aatgtgcct gagttgagt aacgcccag aaacagatt 
      acgcaattt gaggaggct ctggagact aaagtggcg 
      tccagttta cagaaaatt gaagaccag atgcagtcg 
      ttttggaat aagttgaat gaaatggag atgtataga 
      tgtagagat atggaagat aaacctaaa ccttctgct 
      aagcctaga agcaaatat tcggataaa catttgatt 
      gaaactagg caaaggaac gaatctagt cagcaacgt 
      gataaaaaa caggcaaaa agtgtttct ctggaggaa 
      gataacgaa ataactgat gctagtcca caaagtcag 
      gaaacgcca aaagcttct ccaattaag gtaatgaga 
      gctcagcct caaataaag gttcctaaa gtcaaaact 
      gagaaaatc gagtctggg attaattta actgaaatt 
      gacgatgac actaccact tctgaaaca cttgcttcc 
      gaagaaaaa atttcggat tcgccccaa gaaaaagta 
      atcaaaccg cctttaaag cctcaacct gttccaaga 
      aagcgccca aaaacaagt aaagatata gtttcaagt 
      aatataata gacgacgag ttgttgcaa cattttaaa 
      ttagaaatc gaaaatatt ctaaaggcg cgtttgcaa 
      gacatgggt gtttcgccc gattggaaa ggtctgcct 
      aatcgttcc tttcagcga gcgttagag atagtaaac 
      catcaagca aatttggtg aaaaaaacg tgtccgaac 
      tttgactca attgcatca caaattgag aaagacgtg 
      gcgaaaaaa atcgataac aaacgtcaa ccaaagaag 
      aaaccgaaa aaactcgcg gttcagatt gagataaaa 
      aatcggcaa atgtacgac actgacact gagagtgaa 
      gtgaaaccg gtcaaagtc aaggagaaa caattctca 
      gcgccttat tcagctgtg atagcggag ttgaagaca 
      gtaacgtcc aaaattgac gcttctgat aaatctgaa 
      gagtcaata aaggacgaa aaacgggtc aaggaggac 
      cagactaag ggcgttttg aagagttac ccttctgtc 
      ggctccctt acgaagaaa aaagtgttg tttaatttg 
      gacaccgaa gagaagaaa gaattgccg gctaaagac 
      ggcgggagc actacaagc atcacaagc tcggttttc 
      gacggcaca ccccggaat gaagaacca aagaagaaa 
      ggcaaagat atcgaagat ttatccgat tttgatttt 
      agcgatatg tag
    mRNA sequence
    • >TC013350 RA
      actttgcgc aagcgtcct ctttaaatt gtgtttact 
      tctaaccct aaaaaactc tttgatgcc ttttttacg 
      aaaattaag ccaaaatag gccccacaa cgaactatg 
      tacaaggga gattgcaag tggcaccat gattacgta 
      cgtctagct tttgacacc ggctttaaa ttcgaaaaa 
      cacaaatcg gacaacttt gacagaaac aagataagt 
      ttaattgat attgatcga gtgattgaa gagagagac 
      tttgcaacc gttgaaacc cttattcct gggataaca 
      aaatttgtt ctagacccg gacctggcg caggtcttg 
      gacgttagt ttcgtaaaa acgttcaaa ttgagccaa 
      ttggcgatt cagtattta ctattttgc aaaaaatat 
      ctcgataat accgtaata gtgttgaaa gatgagttg 
      aagaaagtt cgaggggaa aacaaagaa ttgaattta 
      tttgttgac gatctcaag gagcacatt gcgactttg 
      atgcgcgaa attgacgaa aaagttgcc ttcaaatgc 
      gaacagtgc cttaaagtt ttctcaagt gaggaattt 
      ttaaattcg catatcagg cgccgccat agtaccccc 
      acacaggtc cccgaaacg gacaaactc cagatggaa 
      atcaaggaa ttgaaaggg cgcctcaac gatgcggag 
      aaaatgatt caaaaggag caccaggag attgactca 
      aatatcagt agggagacg gattttaag aaaattgaa 
      gatttgcag caaaagttt gaggatttg cggctgcag 
      gtccagtcc gaattgaag gttttgcag acgcagcat 
      aacttccag gagaagtat gaaaagtgg tttgagaaa 
      atggccctt caggggggc agtttgggg cgcaggagg 
      gagagcatg acgcagact gaggagggg gcgtcgagt 
      cgtgttgtt atgtatcag gaaaaaaat aaagaaaat 
      gtgcctgag ttgagtaac gcccagaaa cagattacg 
      caatttgag gaggctctg gagactaaa gtggcgtcc 
      agtttacag aaaattgaa gaccagatg cagtcgttt 
      tggaataag ttgaatgaa atggagatg tatagatgt 
      agagatatg gaagataaa cctaaacct tctgctaag 
      cctagaagc aaatattcg gataaacat ttgattgaa 
      actaggcaa aggaacgaa tctagtcag caacgtgat 
      aaaaaacag gcaaaaagt gtttctctg gaggaagat 
      aacgaaata actgatgct agtccacaa agtcaggaa 
      acgccaaaa gcttctcca attaaggta atgagagct 
      cagcctcaa ataaaggtt cctaaagtc aaaactgag 
      aaaatcgag tctgggatt aatttaact gaaattgac 
      gatgacact accacttct gaaacactt gcttccgaa 
      gaaaaaatt tcggattcg ccccaagaa aaagtaatc 
      aaaccgcct ttaaagcct caacctgtt ccaagaaag 
      cgcccaaaa acaagtaaa gatatagtt tcaagtaat 
      ataatagac gacgagttg ttgcaacat tttaaatta 
      gaaatcgaa aatattcta aaggcgcgt ttgcaagac 
      atgggtgtt tcgcccgat tggaaaggt ctgcctaat 
      cgttccttt cagcgagcg ttagagata gtaaaccat 
      caagcaaat ttggtgaaa aaaacgtgt ccgaacttt 
      gactcaatt gcatcacaa attgagaaa gacgtggcg 
      aaaaaaatc gataacaaa cgtcaacca aagaagaaa 
      ccgaaaaaa ctcgcggtt cagattgag ataaaaaat 
      cggcaaatg tacgacact gacactgag agtgaagtg 
      aaaccggtc aaagtcaag gagaaacaa ttctcagcg 
      ccttattca gctgtgata gcggagttg aagacagta 
      acgtccaaa attgacgct tctgataaa tctgaagag 
      tcaataaag gacgaaaaa cgggtcaag gaggaccag 
      actaagggc gttttgaag agttaccct tctgtcggc 
      tcccttacg aagaaaaaa gtgttgttt aatttggac 
      accgaagag aagaaagaa ttgccggct aaagacggc 
      gggagcact acaagcatc acaagctcg gttttcgac 
      ggcacaccc cggaatgaa gaaccaaag aagaaaggc 
      aaagatatc gaagattta tccgatttt gattttagc 
      gatatgtag agttgttat ttttgtagt ctctgaata 
      aagttattt caaagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY