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Gene information
General information
TC014419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10245254..10249315
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014419
    iBeetle screen iB_02800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014419 RA
      MSVSKDGSQ AMEKRSEGS SILSVIFSA VKKASIVGI 
      VYFAGYMQW SVAWFIGPI VLFVIRDQW KKASDRKRN 
      IAKAAALAS EKDVVLARL DDLPAWVFF PDVERAEWL 
      NRIIKQVWP NINHYTRDL IRDTIQPIL KESLETYKL 
      SGFKFERII LGTVPFRIG GVKVYDKNV ARNEIIMDL 
      DIFYAGDCD ITFYLAGIK GGIRDFQLH GMLRVVMKP 
      LITTIPLVG GLQVFFLNN PDIDFDLIG IADLLDMPG 
      LSDILRRIV VETVASMMV LPNKFPIKL SDDVDAMEL 
      KAPEPEGVL RVHVVEAKH LMKKDIGVL GKGKSDPYA 
      VVTLGAQEF KTKVIDNSV DPKWDFWCE FNVLESDGQ 
      QLYIHLWDK DETSDDETL GRATIEVSN IVKKGQDDL 
      WVTLEQAKH GMVHLRLTW LTLSDNYSD LKAALEETQ 
      QLRVTSMST ALLTIFLDS AKNLPQARA STKPDPYAV 
      LKVGNTTKE TKVLERTIH PVWEQGFSF LVANPESDT 
      LYLTIIDRK TTNELGQVT YNISKLAKK TKMEVYKEP 
      FSLLKSGPE SKVIWSMHL RVLKRAEGV DDTDSGDLP 
      SLQREDSKV LTSDDVPPT PNPTPEINE SATEPIPNY 
      DEMIKATAA TVASTPIKE SELTHRSPS VTSSAGAHD 
      LGRIQLTIR YSVQRQRLI VVVHQIANI PLKDPSNIP 
      DPYVKLYLL PERAKDTKR KTHVVKDNC NPIFDESFE 
      YILSQGELN TKQLEVTVA SQKQLFYSS SNILGMVII 
      DFEKLNVSQ PYNAWFDLT PESDRNHR
    CDS sequence
    • >TC014419 RA
      atgagtgtg agtaaagac gggagtcaa gccatggag 
      aaacgctcc gagggctcc agcatcttg agcgttatt 
      ttcagcgca gtcaaaaag gcctccatt gtcggcatt 
      gtgtacttt gcgggttac atgcaatgg tcggtcgcg 
      tggttcatt ggccccatt gtccttttc gtgatcaga 
      gaccaatgg aaaaaagcc agcgacagg aaacgcaac 
      atagcaaaa gcggcagct ttagcgagc gaaaaagac 
      gttgttttg gcgagactt gatgatctt ccagcctgg 
      gtgtttttc cctgacgtt gaaagagcc gagtggctc 
      aacagaatt attaaacag gtttggcct aacattaac 
      cactacaca cgcgactta atcagggac accatccaa 
      ccaattttg aaagaaagc ctagaaacg tataaactg 
      tccggtttc aaatttgaa cgcattatc ctaggcacc 
      gtgccgttc cgaatcggg ggtgttaaa gtatacgat 
      aaaaatgta gccaggaac gaaattatc atggatttg 
      gacattttt tatgccggc gattgcgac attacgttc 
      tacttggcc gggatcaag ggcggaatc cgcgatttc 
      cagctccac ggcatgctc cgcgttgtg atgaagccc 
      ctgatcacc acaataccg ctggtgggt ggcctccaa 
      gtcttcttc ctcaacaac cccgatata gacttcgac 
      cttatcggc atcgccgac ttgctcgac atgcccggc 
      ttgagcgac atcttgcgg cgcatcgtg gtggagacc 
      gtggcctca atgatggtc ctccccaat aaattcccg 
      atcaagttg agcgacgac gtggatgcc atggaacta 
      aaagccccg gaaccggaa ggcgttttg cgcgtccat 
      gtggtcgag gccaagcac ctgatgaag aaggacata 
      ggagtgcta ggtaaaggt aaatccgac ccttacgca 
      gtggtcact ttaggtgcc caagagttc aaaacgaag 
      gttatcgac aatagcgtg gacccgaag tgggatttc 
      tggtgcgag tttaatgtg ttggaatcg gacggccaa 
      caactttat atccatctg tgggataag gacgaaacg 
      tcggatgat gagactttg ggcagggcc acgattgaa 
      gtatccaac atcgtaaag aaaggccaa gacgacttg 
      tgggtcact ttggagcaa gccaaacac ggcatggtc 
      catttacgt ttgacttgg ctcacgtta agcgacaat 
      tactccgat ttgaaggct gccctcgag gaaacgcaa 
      caattacga gtcacatcg atgagtacg gccttgtta 
      acaatcttc cttgattcg gccaaaaac cttccgcaa 
      gcccgcgcg agcacaaaa cccgaccct tacgccgtc 
      ctcaaagtc ggcaatacg accaaagag accaaagtc 
      ttggaacgc acaatacac ccagtttgg gagcaaggc 
      ttctccttc cttgtggcc aaccccgaa tccgacaca 
      ctttacttg acaataatt gatcggaag acaacaaat 
      gagctcggc caagtcact tacaacatc agtaaactg 
      gcgaaaaag accaaaatg gaggtttac aaagaaccg 
      ttttcgtta cttaaatcc ggaccggag agtaaagtc 
      atctggtcg atgcactta agggtactt aaaagggcc 
      gaaggagtc gatgacact gactctggt gatcttcca 
      tctttgcaa cgcgaggat tctaaagta ttaacaagt 
      gacgatgtt cctccaacc ccaaaccca actcccgaa 
      ataaatgaa agtgccact gaaccgatc cctaattac 
      gacgaaatg attaaagcg actgcagca acagtagcg 
      agtactcca atcaaagag agcgaactc actcatcgg 
      agcccgagt gtcacttca tcggcgggt gcccacgac 
      ttgggtcgt attcaattg acaatcaga tatagcgtc 
      caacgccag cgtctcatt gttgtagtg caccaaatc 
      gcaaatata cccctgaaa gacccctcc aatatcccc 
      gacccctac gtcaagctc tacctcctt ccagaacgg 
      gctaaggat acgaaacgc aaaacgcac gtcgttaag 
      gacaactgc aatccgatt tttgatgaa agttttgaa 
      tatatcctc tcccaaggc gaactgaac actaaacaa 
      ctcgaagtt actgtcgca tcgcaaaaa cagctgttt 
      tacagcagc agtaacatt ttgggaatg gtgattatc 
      gattttgag aaacttaat gtaagtcag ccgtacaac 
      gcttggttt gatcttacg ccggagtct gaccggaat 
      caccgctaa
    mRNA sequence
    • >TC014419 RA
      gcaactttt accgatttt gtaatgttg taaatatag 
      tttgtgatg tcatattga acaacacga ggcggggcg 
      gttgtaatc tcacaacaa attgaaatg cttcaaaga 
      taacccttt agtagctct ccatttcct tggaagcaa 
      agatgagtg tgagtaaag acgggagtc aagccatgg 
      agaaacgct ccgagggct ccagcatct tgagcgtta 
      ttttcagcg cagtcaaaa aggcctcca ttgtcggca 
      ttgtgtact ttgcgggtt acatgcaat ggtcggtcg 
      cgtggttca ttggcccca ttgtccttt tcgtgatca 
      gagaccaat ggaaaaaag ccagcgaca ggaaacgca 
      acatagcaa aagcggcag ctttagcga gcgaaaaag 
      acgttgttt tggcgagac ttgatgatc ttccagcct 
      gggtgtttt tccctgacg ttgaaagag ccgagtggc 
      tcaacagaa ttattaaac aggtttggc ctaacatta 
      accactaca cacgcgact taatcaggg acaccatcc 
      aaccaattt tgaaagaaa gcctagaaa cgtataaac 
      tgtccggtt tcaaatttg aacgcatta tcctaggca 
      ccgtgccgt tccgaatcg ggggtgtta aagtatacg 
      ataaaaatg tagccagga acgaaatta tcatggatt 
      tggacattt tttatgccg gcgattgcg acattacgt 
      tctacttgg ccgggatca agggcggaa tccgcgatt 
      tccagctcc acggcatgc tccgcgttg tgatgaagc 
      ccctgatca ccacaatac cgctggtgg gtggcctcc 
      aagtcttct tcctcaaca accccgata tagacttcg 
      accttatcg gcatcgccg acttgctcg acatgcccg 
      gcttgagcg acatcttgc ggcgcatcg tggtggaga 
      ccgtggcct caatgatgg tcctcccca ataaattcc 
      cgatcaagt tgagcgacg acgtggatg ccatggaac 
      taaaagccc cggaaccgg aaggcgttt tgcgcgtcc 
      atgtggtcg aggccaagc acctgatga agaaggaca 
      taggagtgc taggtaaag gtaaatccg acccttacg 
      cagtggtca ctttaggtg cccaagagt tcaaaacga 
      aggttatcg acaatagcg tggacccga agtgggatt 
      tctggtgcg agtttaatg tgttggaat cggacggcc 
      aacaacttt atatccatc tgtgggata aggacgaaa 
      cgtcggatg atgagactt tgggcaggg ccacgattg 
      aagtatcca acatcgtaa agaaaggcc aagacgact 
      tgtgggtca ctttggagc aagccaaac acggcatgg 
      tccatttac gtttgactt ggctcacgt taagcgaca 
      attactccg atttgaagg ctgccctcg aggaaacgc 
      aacaattac gagtcacat cgatgagta cggccttgt 
      taacaatct tccttgatt cggccaaaa accttccgc 
      aagcccgcg cgagcacaa aacccgacc cttacgccg 
      tcctcaaag tcggcaata cgaccaaag agaccaaag 
      tcttggaac gcacaatac acccagttt gggagcaag 
      gcttctcct tccttgtgg ccaaccccg aatccgaca 
      cactttact tgacaataa ttgatcgga agacaacaa 
      atgagctcg gccaagtca cttacaaca tcagtaaac 
      tggcgaaaa agaccaaaa tggaggttt acaaagaac 
      cgttttcgt tacttaaat ccggaccgg agagtaaag 
      tcatctggt cgatgcact taagggtac ttaaaaggg 
      ccgaaggag tcgatgaca ctgactctg gtgatcttc 
      catctttgc aacgcgagg attctaaag tattaacaa 
      gtgacgatg ttcctccaa ccccaaacc caactcccg 
      aaataaatg aaagtgcca ctgaaccga tccctaatt 
      acgacgaaa tgattaaag cgactgcag caacagtag 
      cgagtactc caatcaaag agagcgaac tcactcatc 
      ggagcccga gtgtcactt catcggcgg gtgcccacg 
      acttgggtc gtattcaat tgacaatca gatatagcg 
      tccaacgcc agcgtctca ttgttgtag tgcaccaaa 
      tcgcaaata tacccctga aagacccct ccaatatcc 
      ccgacccct acgtcaagc tctacctcc ttccagaac 
      gggctaagg atacgaaac gcaaaacgc acgtcgtta 
      aggacaact gcaatccga tttttgatg aaagttttg 
      aatatatcc tctcccaag gcgaactga acactaaac 
      aactcgaag ttactgtcg catcgcaaa aacagctgt 
      tttacagca gcagtaaca ttttgggaa tggtgatta 
      tcgattttg agaaactta atgtaagtc agccgtaca 
      acgcttggt ttgatctta cgccggagt ctgaccgga 
      atcaccgct aaccgtcaa ttatttatc aatctactt 
      atacaagtg atgtaatac cgtttttaa cttttttat 
      gtgttgcta tttatgcca gttatgtat taattaata 
      aagcgatgt tgagctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY