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Gene information
General information
TC014371 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9877843..9886109
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014371
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014371 RA
      MPPVVSISS DEVDDDVAF KVLNQDNLP ITIIGEAIL 
      KPFGRENQK QKPNGIVYP TRRIDTNLE ENKQKTFTR 
      SNGHINTTQ DKNNYNLKK NFSNSHGNL TSATKIKPQ 
      EQVDTKFTK TLDAKLRKL QKESKSKND NGRKPFVTT 
      VKKGQFLEP PPEIATLIG IKVEEPKQK EIKKLYAYA 
      SEPRVLNRT PAKNVHKAK CEAAAKAAA CVVAGVAGI 
      QTDLDVNSN VNKRALDVP LPYSNLMFD RRICRGSNF 
      APTLSGHMG DGESAAARA AEARRRALA RRKAKNQQV 
      RAAQLRLGS PPPVPGRKH EPVQTELYL EEIFNNPPT 
      SDICTQTEL FLERPLSPY YVPAKVGID AETQIYPGD 
      LFDFDMEVQ PILEVLVGK TVEQALIEV LEEEELAAL 
      REQQRKFLE VRSAETAEA QRLEERERR LAKEKDRRI 
      AEFEDGYRL QKEMEERIA ASVLMQGYI SDLLPSVLE 
      GLENEGFLM DSIKQDIDE SFMPWLMKE VTHELKDIV 
      SSRDVLTDI VREILENRA EIYKALNQT PRSPPSEEE 
      EGPTQEDIV LADHLKLQE EISKKIAEE Q
    CDS sequence
    • >TC014371 RA
      atgcctccg gtagtttcg atttcttcg gacgaagtc 
      gacgatgat gtggccttt aaagtcctc aaccaagac 
      aacctcccc ataaccata ataggggag gcaattctg 
      aaacccttc ggccgcgaa aaccaaaaa cagaaaccg 
      aacggaatc gtctatcca actcgacgc attgacacc 
      aacttggaa gaaaacaaa cagaaaacc tttaccaga 
      tcaaacggc cacatcaac accactcaa gacaaaaac 
      aactacaac ctcaagaag aactttagc aattcccac 
      gggaattta acgagcgct acaaaaatc aaaccgcaa 
      gaacaagtc gatacaaaa ttcaccaaa acgctcgac 
      gctaaactg cgaaagctc cagaaagag tcgaaaagt 
      aagaatgat aatggcagg aagccgttt gttacgacg 
      gtaaagaag gggcagttt ctggagccg cccccggaa 
      atagccact ttgattggc attaaagtg gaggaaccg 
      aagcagaaa gaaattaaa aagttgtac gcgtatgca 
      agcgagccg agggtgttg aacagaaca ccggcgaag 
      aacgttcac aaggcgaaa tgtgaggcg gcggcgaag 
      gcggcggcg tgcgtggtt gccggggtg gcggggatt 
      caaaccgat ctagatgtt aacagtaat gtcaacaaa 
      cgagctttg gatgtgcct ttgccttat tcgaatctc 
      atgttcgac cggcggatt tgtaggggc agtaatttc 
      gctcctaca ttatccgga cacatgggt gatggcgag 
      tcggctgca gctcgcgcc gccgaagct cgaagacgg 
      gccctcgca cggagaaaa gccaaaaac caacaagtt 
      agggccgca caactgcgt ctaggatcg ccgccgccc 
      gtcccaggg agaaagcac gaacccgtc caaaccgag 
      ctctatctc gaagagatc ttcaacaat ccgccgacc 
      tcggacatc tgcacacaa accgaactg ttcctcgaa 
      aggcctctg tcgccttac tatgtcccg gctaaagtg 
      gggatcgat gcagaaacg cagatttat ccgggagat 
      ttgttcgac ttcgatatg gaggtgcag ccgattttg 
      gaagttttg gttgggaaa acggtggaa caggcgttg 
      attgaagtc ttggaagag gaggagttg gcggcgttg 
      agggaacaa caaaggaag ttcttggag gtcaggtca 
      gcggaaact gcggaggcc cagaggctg gaagagagg 
      gagaggaga ctggcgaag gagaaggac cggagaatc 
      gcagagttt gaagatggg taccggctg cagaaggag 
      atggaggag aggatagcg gcgtcggtg ctgatgcag 
      ggatacata tcagatttg cttccgtcg gttttagaa 
      ggattagag aacgaaggg ttcctcatg gatagtatc 
      aaacaagac atcgacgag tccttcatg ccttggctg 
      atgaaggaa gtcacacac gagctgaag gatatagtg 
      agcagtcga gacgtcctt acagatatc gttcgagag 
      atcctcgaa aaccgcgcc gaaatctac aaggccttg 
      aaccaaacc ccgcgctcc ccgcccagc gaagaagaa 
      gaagggccc acccaggaa gatattgtt ctcgccgac 
      catttgaag ctgcaggaa gaaatatcg aagaagatc 
      gccgaagaa caataa
    mRNA sequence
    • >TC014371 RA
      gtggtgcgg ccaactttt cggtttctg gcgggagtt 
      tgtgaaagt ggcgaaatg gcagtgcag gagaaaggg 
      gtttcacgg agacgaacg attcatcca aaggcgatg 
      ttggagata aacgacaat ccgttgcaa gatgttaat 
      gttaatatt ttcgaagac ggggaagtc gcttacacg 
      tactccagc tcgccgaga gcgctttat accaacagg 
      aagatggtg attggaagc aagccgccc ccagtagcc 
      atgcgccgc cacctcttc aaaccgcct tcgaatgga 
      caacggtca gttgtgaaa gaccggtca atcgtaaca 
      tatgtcggc gtcgcgacg ccgatccga aagaccgac 
      gaaataatg tgatagtta aaagtgagc ttttccaca 
      caaaaagtg aaaacccaa tatcgatac taaagaaaa 
      ttatttttg gagtagcta gaaccgaat aatagatga 
      tagaaagtt tatttttag tccaacttt ttcaataat 
      taattatag gcaatataa tcgaatata agcaataag 
      tagattaga atttctgga attggaagg ctgaaaaag 
      agactgtca agatgtttt attgaaatt gttaccgat 
      gaccgactt agcgatgcc tccggtagt ttcgatttc 
      ttcggacga agtcgacga tgatgtggc ctttaaagt 
      cctcaacca agacaacct ccccataac cataatagg 
      ggaggcaat tctgaaacc cttcggccg cgaaaacca 
      aaaacagaa accgaacgg aatcgtcta tccaactcg 
      acgcattga caccaactt ggaagaaaa caaacagaa 
      aacctttac cagatcaaa cggccacat caacaccac 
      tcaagacaa aaacaacta caacctcaa gaagaactt 
      tagcaattc ccacgggaa tttaacgag cgctacaaa 
      aatcaaacc gcaagaaca agtcgatac aaaattcac 
      caaaacgct cgacgctaa actgcgaaa gctccagaa 
      agagtcgaa aagtaagaa tgataatgg caggaagcc 
      gtttgttac gacggtaaa gaaggggca gtttctgga 
      gccgccccc ggaaatagc cactttgat tggcattaa 
      agtggagga accgaagca gaaagaaat taaaaagtt 
      gtacgcgta tgcaagcga gccgagggt gttgaacag 
      aacaccggc gaagaacgt tcacaaggc gaaatgtga 
      ggcggcggc gaaggcggc ggcgtgcgt ggttgccgg 
      ggtggcggg gattcaaac cgatctaga tgttaacag 
      taatgtcaa caaacgagc tttggatgt gcctttgcc 
      ttattcgaa tctcatgtt cgaccggcg gatttgtag 
      gggcagtaa tttcgctcc tacattatc cggacacat 
      gggtgatgg cgagtcggc tgcagctcg cgccgccga 
      agctcgaag acgggccct cgcacggag aaaagccaa 
      aaaccaaca agttagggc cgcacaact gcgtctagg 
      atcgccgcc gcccgtccc agggagaaa gcacgaacc 
      cgtccaaac cgagctcta tctcgaaga gatcttcaa 
      caatccgcc gacctcgga catctgcac acaaaccga 
      actgttcct cgaaaggcc tctgtcgcc ttactatgt 
      cccggctaa agtggggat cgatgcaga aacgcagat 
      ttatccggg agatttgtt cgacttcga tatggaggt 
      gcagccgat tttggaagt tttggttgg gaaaacggt 
      ggaacaggc gttgattga agtcttgga agaggagga 
      gttggcggc gttgaggga acaacaaag gaagttctt 
      ggaggtcag gtcagcgga aactgcgga ggcccagag 
      gctggaaga gagggagag gagactggc gaaggagaa 
      ggaccggag aatcgcaga gtttgaaga tgggtaccg 
      gctgcagaa ggagatgga ggagaggat agcggcgtc 
      ggtgctgat gcagggata catatcaga tttgcttcc 
      gtcggtttt agaaggatt agagaacga agggttcct 
      catggatag tatcaaaca agacatcga cgagtcctt 
      catgccttg gctgatgaa ggaagtcac acacgagct 
      gaaggatat agtgagcag tcgagacgt ccttacaga 
      tatcgttcg agagatcct cgaaaaccg cgccgaaat 
      ctacaaggc cttgaacca aaccccgcg ctccccgcc 
      cagcgaaga agaagaagg gcccaccca ggaagatat 
      tgttctcgc cgaccattt gaagctgca ggaagaaat 
      atcgaagaa gatcgccga agaacaata acggtatga 
      ctcattcgt cataactta tgcaatgtc gcatccctt 
      aataaacct cattcaaaa ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY