Status
Please wait ...
Gene information
General information
TC031145
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013483
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 1059290..1069694
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031145
    iBeetle screen iB_02797
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    remarks: animals lost, remarks: animals lost,
    Adult morphology
    remarks: animals lost, remarks: animals lost,
    Analysis of embryonic musculature and early development
    remarks: animals lost, (egglay)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031145 RA
      MPFACLIRC HIFISHPAA IFGFLSVKK WLLRKKHQI 
      ELARKRGWK GYWVCLKGT TLLFYPCDS REGRSVEAA 
      PKHLIIVDG AIMQPIPEH PKRDYIFCL STAFGDAYL 
      FQAPCQVEL ENWVNSIHS ACAAAFARH RGKTGTLHL 
      LQEEIFRLE KAIETDYKL KHMAEMQQS VVGDSDARQ 
      QIANQVVQW EENLERLHC EQFRLRCYM ASLQSGELP 
      NPKSLLTHV SRATKNTLN KLGVFTVSS FHAFICARS 
      PSLLNNLLA GRGATKRRP PLLSRSNSG SSRRSLQIN 
      SREESEKTV KVSLPDGNS ATVYVRDSM SVEEFLASA 
      CARKNLSCT EHFIRVKKR RDMEDHNYF VPHRSDLIE 
      TYLHTHEVV EVCAKILYQ VELSRSTLE QMWGFSVEA 
      ELVENADRQ DELCCYVSR VEDKSVAMQ NGIIKGDEI 
      MVINGAIVS DLDMMYLES VLQEELALC MMMRSSRTE 
      PPELVGILR ATDDIIESL VCPPPPSEP PVISEEMIS 
      GLIVPAPGW SKDRYSTES ENPPSSMTD SGIKVSSRT 
      NSFEIENLL KTAEQVTGF CRSPVETRK SSPTGSLVS 
      NTSQTLLTP SRQLSDAEK LRKVILELV DTERAYVKH 
      LNNLLENYL EPLKRETFL SNAEINALF GNIQEIVTF 
      QRQFLQNLE EALDLEPDF HKFEYSSQF KNVLFSIGS 
      AFLYYVNHF KLYSSFCAS HSKAQKVLH PNEGNQALQ 
      EFLAARNPK QQHSSTLES YLIKPIQRI LKYPLLLQQ 
      LRNLTDPNT DEHQHLVEA LKGMEKVAE HINEMQRIH 
      EEYGAIFDH LFRQHQKSS KQSIDLSPG DLLYYGGVE 
      WLNISDFLG KIKKGLELH AMCFVFKTA VVFLCKERL 
      RQKKKLMGV SSKGSSSEV EIIRYQVLI PVTEVQVRA 
      SSAKDMDSH FLWELIHLR SQLQRRSEK VYVLSNSTA 
      DFRNAFLKT IRQIIRESV RNMSIPATK PGTGPGILL 
      VTGHKGENL NSQTLERPA TKTVIVQGS HTLGKTKKK 
      SSQRHSAGN IDYDNISQE GDEPTTTFR SRSKTVGDA 
      ADPPKRSDH DRNDTGAKS EGEEDSQGG KKATLGRTP 
      NHLTLSTTS TLSAGSTGS QARLIQSSH APETFQPMQ 
      TEELGSPVW KPRDSSLEA TSTTLPRKG RDHSSALSA 
      SRKSLVETT TGLEIHNAY SHQ
    • >TC031145 RB
      MPFACLIRC HIFISHPAA IFGFLSVKK WLLRKKHQI 
      ELARKRGWK GYWVCLKGT TLLFYPCDS REGRSVEAA 
      PKHLIIVDG AIMQPIPEH PKRDYIFCL STAFGDAYL 
      FQAPCQVEL ENWVNSIHS ACAAAFARH RGKTGTLHL 
      LQEEIFRLE KAIETDYKL KHMAEMQQS VVGDSDARQ 
      QIANQVVQW EENLERLHC EQFRLRCYM ASLQSGELP 
      NPKSLLTHV SRATKNTLN KLGVFTVSS FHAFICARS 
      PSLLNNLLA GRGATKRRP PLLSRSNSG SSRRSLQIN 
      SREESEKTV KVSLPDGNS ATVYVRDSM SVEEFLASA 
      CARKNLSCT EHFIRVKKR RDMEDHNYF VPHRSDLIE 
      TYLHTHEVV EVCAKILYQ VELSRSTLE QMWGFSVEA 
      ELVENADRQ DELCCYVSR VEDKSVAMQ NGIIKGDEI 
      MVINGAIVS DLDMMYLES VLQEELALC MMMRSSRTE 
      PPELVGILR ATDDIIESL VCPPPPSEP PVISEEMIS 
      GLIVPAPGK DRYSTESEN PPSSMTDSG IKVSSRTNS 
      FEIENLLKT AEQVTGFCR SPVETRKSS PTGSLVSNT 
      SQTLLTPSR QLSDAEKLR KVILELVDT ERAYVKHLN 
      NLLENYLEP LKRETFLSN AEINALFGN IQEIVTFQR 
      QFLQNLEEA LDLEPDFHK FEYSSQFKN VLFSIGSAF 
      LYYVNHFKL YSSFCASHS KAQKVLHPN EGNQALQEF 
      LAARNPKQQ HSSTLESYL IKPIQRILK YPLLLQQLR 
      NLTDPNTDE HQHLVEALK GMEKVAEHI NEMQRIHEE 
      YGAIFDHLF RQHQKSSKQ SIDLSPGDL LYYGGVEWL 
      NISDFLGKI KKGLELHAM CFVFKTAVV FLCKERLRQ 
      KKKLMGVSS KGSSSEVEI IRYQVLIPV TEVQVRASS 
      AKDMDSHFL WELIHLRSQ LQRRSEKVY VLSNSTADF 
      RNAFLKTIR QIIRESVRN MSIPATKPG TGPGILLVT 
      GHKGENLNS QTLERPATK TVIVQGSHT LGKTKKKSS 
      QRHSAGNID YDNISQEGD EPTTTFRSR SKTVGDAAD 
      PPKRSDHDR NDTGAKSEG EEDSQGGKK ATLGRTPNH 
      LTLSTTSTL SAGSTGSQA RLIQSSHAP ETFQPMQTE 
      ELGSPVWKP RDSSLEATS TTLPRKGRD HSSALSASR 
      KSLVETTTG LEIHNAYSH Q
    CDS sequence
    • >TC031145 RA
      atgccattt gcttgccta attaggtgc catattttc 
      atcagccac ccggccgca atcttcgga ttcctcagc 
      gtgaagaaa tggctcttg cgaaagaag caccagatc 
      gaactggcc cgcaaacgc ggctggaag ggctactgg 
      gtgtgcttg aagggcacc actctgctg ttctacccc 
      tgcgactcg cgcgagggc cgctccgtg gaggcggcg 
      cccaaacac ctcatcatc gtcgacggc gccatcatg 
      cagcccatc ccagaacac cccaaaaga gactacatt 
      ttctgcctg agtacggcg ttcggtgac gcctacttg 
      ttccaagcg ccttgtcag gtcgagctg gagaactgg 
      gtgaatagc attcacagc gcctgtgct gcggctttt 
      gctcgccat cgcggcaaa accggcact ctgcatctg 
      ctgcaagag gagattttc cgcttggag aaagccatc 
      gagaccgac tacaagctc aaacacatg gccgaaatg 
      cagcaaagc gtcgtggga gactccgac gctcggcag 
      caaatcgcc aatcaggtg gtacaatgg gaggaaaac 
      ctggaaagg ctgcattgc gaacagttt cggctccgg 
      tgctacatg gccagtctg cagagcgga gaactgccc 
      aaccctaag tccttgctg acgcacgtc tcgagagca 
      acgaagaat accttaaat aagttggga gtgtttacc 
      gtttcctcc tttcatgcc tttatttgt gcgcggagt 
      ccctcttta ctcaacaac ctgctcgca ggccgagga 
      gcgacgaag agaaggccg ccgcttctg tccaggtcc 
      aacagcggc tcgagcagg aggtctttg cagataaat 
      tcccgggaa gagagcgaa aagaccgtt aaggtgtca 
      ctgccagac gggaattcg gccacggtc tacgtgcgc 
      gactccatg tcggtggag gagttcctg gcgagtgct 
      tgcgcgagg aaaaacctc agctgcacg gaacacttc 
      ataagggtg aagaagagg cgggacatg gaggaccac 
      aactacttt gtgcctcac agaagcgac ctgatcgag 
      acttacctc cacacgcac gaagtcgtc gaggtctgt 
      gcgaaaatc ctgtaccaa gtggagctc tcgcggagc 
      acgttggag cagatgtgg ggcttcagc gtcgaggcc 
      gaactggtc gaaaacgcc gacagacag gacgagctt 
      tgctgctac gtcagtcgc gtcgaagat aaaagcgta 
      gcgatgcaa aacggcatc ataaagggc gacgaaatc 
      atggtcata aacggcgcc atcgtgtcc gacctggac 
      atgatgtac ctcgaaagt gtcttgcag gaggagctg 
      gctttgtgt atgatgatg aggtcgtcc cggaccgag 
      cctccggaa ctggttgga atcctccgg gcgactgac 
      gacatcatc gaaagctta gtctgtcct ccacctccg 
      tcggaaccc ccagtcatc agcgaggaa atgatctcc 
      ggactcatc gtgcctgct ccaggttgg agcaaagac 
      cgctactct accgagtct gaaaatccg ccttcgtcg 
      atgaccgat tccgggata aaggtgagt tcgcgtaca 
      aactcgttc gaaatcgaa aacctgctg aaaactgct 
      gaacaagtg accggtttc tgtcgctca cctgttgaa 
      acgcgcaaa tcgagtcct acaggaagt ttagtcagc 
      aacacttcc cagactcta ctaactccc tctcggcag 
      ctcagcgac gccgagaag ttgcgaaaa gtcatcttg 
      gagttggta gatacggag cgagcgtac gtcaagcat 
      ctcaacaat ttattggag aactacttg gagccgctg 
      aagcgggag acgttcctg tccaacgcg gaaatcaac 
      gctttgttc ggaaacatc caggagatc gtcacgttc 
      cagagacag ttccttcag aacctcgaa gaggctctc 
      gatcttgag ccggatttc cataaattt gaatacagc 
      agccaattt aaaaatgta ttgttttcc atcgggagc 
      gcatttttg tattatgta aatcacttc aaattatac 
      agctcgttc tgcgcgagc cacagcaaa gcgcagaaa 
      gttttgcat ccgaacgaa ggcaatcaa gctctgcag 
      gaattcctc gcggcccgt aatcccaaa cagcaacat 
      tcctccact ctagagtct tatctcata aaaccgatt 
      cagcggatt ctcaagtac cctctgctc ttgcaacag 
      ttgcggaac ttgaccgac cccaacacc gacgaacac 
      caacactta gtcgaggcc ctcaaagga atggagaaa 
      gtggccgaa cacatcaac gagatgcag cgaatccac 
      gaggagtac ggggctatt ttcgaccac ctcttccgc 
      caacaccaa aagagttcg aagcagagt atcgatcta 
      agtccggga gatttatta tattatgga ggagtggaa 
      tggctgaac atatcggat ttcctgggg aaaatcaag 
      aaagggctt gaactacac gcgatgtgc ttcgtgttt 
      aaaaccgcg gtcgttttt ctctgcaag gaacgtctt 
      cgccagaaa aagaaacta atgggcgtt tccagtaag 
      ggttcctca tccgaagtc gaaattatt cgctatcaa 
      gtgctcatt cctgtaacc gaagttcaa gtacgagct 
      tcctccgct aaggatatg gattctcat ttcctgtgg 
      gaactgatt cacttaaga agtcaatta cagaggaga 
      tcagagaag gtctacgtt ttgtctaat agcactgct 
      gacttcaga aacgccttt cttaaaacc atcagacaa 
      attatcagg gaatcggtt aggaatatg tcaataccg 
      gcgacgaaa ccgggcacg ggaccggga atcctcctc 
      gtcacagga cacaaaggg gagaatctc aacagtcaa 
      acactggag cggcccgca acgaaaaca gtcatagtg 
      caaggatca cacactttg ggcaaaacg aaaaagaag 
      tcctcgcaa aggcactcg gccgggaat atagactac 
      gacaacatc agccaggaa ggggacgaa cccaccacg 
      accttcagg tcccggagt aaaacagta ggagatgcc 
      gcagatcct cccaaacgc agtgaccac gaccggaac 
      gacaccgga gccaaatcc gagggcgag gaggactcc 
      caagggggc aagaaggcc actctcggc cggaccccc 
      aaccaccta actctcagc acgacctcg actttgtct 
      gccggcagt accggaagc caagcccgc ttgatccaa 
      tcgtcgcac gccccggaa accttccaa cccatgcag 
      accgaagaa ctagggtcg cctgtgtgg aagccaaga 
      gacagtagt ttagaggca acctcaacc accctcccg 
      cgcaaaggc agagaccac agttcggct ttaagcgcc 
      tcgcgtaag tccctcgtc gagacaaca accgggcta 
      gagatacac aatgcgtat agccaccag tag
    • >TC031145 RB
      atgccattt gcttgccta attaggtgc catattttc 
      atcagccac ccggccgca atcttcgga ttcctcagc 
      gtgaagaaa tggctcttg cgaaagaag caccagatc 
      gaactggcc cgcaaacgc ggctggaag ggctactgg 
      gtgtgcttg aagggcacc actctgctg ttctacccc 
      tgcgactcg cgcgagggc cgctccgtg gaggcggcg 
      cccaaacac ctcatcatc gtcgacggc gccatcatg 
      cagcccatc ccagaacac cccaaaaga gactacatt 
      ttctgcctg agtacggcg ttcggtgac gcctacttg 
      ttccaagcg ccttgtcag gtcgagctg gagaactgg 
      gtgaatagc attcacagc gcctgtgct gcggctttt 
      gctcgccat cgcggcaaa accggcact ctgcatctg 
      ctgcaagag gagattttc cgcttggag aaagccatc 
      gagaccgac tacaagctc aaacacatg gccgaaatg 
      cagcaaagc gtcgtggga gactccgac gctcggcag 
      caaatcgcc aatcaggtg gtacaatgg gaggaaaac 
      ctggaaagg ctgcattgc gaacagttt cggctccgg 
      tgctacatg gccagtctg cagagcgga gaactgccc 
      aaccctaag tccttgctg acgcacgtc tcgagagca 
      acgaagaat accttaaat aagttggga gtgtttacc 
      gtttcctcc tttcatgcc tttatttgt gcgcggagt 
      ccctcttta ctcaacaac ctgctcgca ggccgagga 
      gcgacgaag agaaggccg ccgcttctg tccaggtcc 
      aacagcggc tcgagcagg aggtctttg cagataaat 
      tcccgggaa gagagcgaa aagaccgtt aaggtgtca 
      ctgccagac gggaattcg gccacggtc tacgtgcgc 
      gactccatg tcggtggag gagttcctg gcgagtgct 
      tgcgcgagg aaaaacctc agctgcacg gaacacttc 
      ataagggtg aagaagagg cgggacatg gaggaccac 
      aactacttt gtgcctcac agaagcgac ctgatcgag 
      acttacctc cacacgcac gaagtcgtc gaggtctgt 
      gcgaaaatc ctgtaccaa gtggagctc tcgcggagc 
      acgttggag cagatgtgg ggcttcagc gtcgaggcc 
      gaactggtc gaaaacgcc gacagacag gacgagctt 
      tgctgctac gtcagtcgc gtcgaagat aaaagcgta 
      gcgatgcaa aacggcatc ataaagggc gacgaaatc 
      atggtcata aacggcgcc atcgtgtcc gacctggac 
      atgatgtac ctcgaaagt gtcttgcag gaggagctg 
      gctttgtgt atgatgatg aggtcgtcc cggaccgag 
      cctccggaa ctggttgga atcctccgg gcgactgac 
      gacatcatc gaaagctta gtctgtcct ccacctccg 
      tcggaaccc ccagtcatc agcgaggaa atgatctcc 
      ggactcatc gtgcctgct ccaggcaaa gaccgctac 
      tctaccgag tctgaaaat ccgccttcg tcgatgacc 
      gattccggg ataaaggtg agttcgcgt acaaactcg 
      ttcgaaatc gaaaacctg ctgaaaact gctgaacaa 
      gtgaccggt ttctgtcgc tcacctgtt gaaacgcgc 
      aaatcgagt cctacagga agtttagtc agcaacact 
      tcccagact ctactaact ccctctcgg cagctcagc 
      gacgccgag aagttgcga aaagtcatc ttggagttg 
      gtagatacg gagcgagcg tacgtcaag catctcaac 
      aatttattg gagaactac ttggagccg ctgaagcgg 
      gagacgttc ctgtccaac gcggaaatc aacgctttg 
      ttcggaaac atccaggag atcgtcacg ttccagaga 
      cagttcctt cagaacctc gaagaggct ctcgatctt 
      gagccggat ttccataaa tttgaatac agcagccaa 
      tttaaaaat gtattgttt tccatcggg agcgcattt 
      ttgtattat gtaaatcac ttcaaatta tacagctcg 
      ttctgcgcg agccacagc aaagcgcag aaagttttg 
      catccgaac gaaggcaat caagctctg caggaattc 
      ctcgcggcc cgtaatccc aaacagcaa cattcctcc 
      actctagag tcttatctc ataaaaccg attcagcgg 
      attctcaag taccctctg ctcttgcaa cagttgcgg 
      aacttgacc gaccccaac accgacgaa caccaacac 
      ttagtcgag gccctcaaa ggaatggag aaagtggcc 
      gaacacatc aacgagatg cagcgaatc cacgaggag 
      tacggggct attttcgac cacctcttc cgccaacac 
      caaaagagt tcgaagcag agtatcgat ctaagtccg 
      ggagattta ttatattat ggaggagtg gaatggctg 
      aacatatcg gatttcctg gggaaaatc aagaaaggg 
      cttgaacta cacgcgatg tgcttcgtg tttaaaacc 
      gcggtcgtt tttctctgc aaggaacgt cttcgccag 
      aaaaagaaa ctaatgggc gtttccagt aagggttcc 
      tcatccgaa gtcgaaatt attcgctat caagtgctc 
      attcctgta accgaagtt caagtacga gcttcctcc 
      gctaaggat atggattct catttcctg tgggaactg 
      attcactta agaagtcaa ttacagagg agatcagag 
      aaggtctac gttttgtct aatagcact gctgacttc 
      agaaacgcc tttcttaaa accatcaga caaattatc 
      agggaatcg gttaggaat atgtcaata ccggcgacg 
      aaaccgggc acgggaccg ggaatcctc ctcgtcaca 
      ggacacaaa ggggagaat ctcaacagt caaacactg 
      gagcggccc gcaacgaaa acagtcata gtgcaagga 
      tcacacact ttgggcaaa acgaaaaag aagtcctcg 
      caaaggcac tcggccggg aatatagac tacgacaac 
      atcagccag gaaggggac gaacccacc acgaccttc 
      aggtcccgg agtaaaaca gtaggagat gccgcagat 
      cctcccaaa cgcagtgac cacgaccgg aacgacacc 
      ggagccaaa tccgagggc gaggaggac tcccaaggg 
      ggcaagaag gccactctc ggccggacc cccaaccac 
      ctaactctc agcacgacc tcgactttg tctgccggc 
      agtaccgga agccaagcc cgcttgatc caatcgtcg 
      cacgccccg gaaaccttc caacccatg cagaccgaa 
      gaactaggg tcgcctgtg tggaagcca agagacagt 
      agtttagag gcaacctca accaccctc ccgcgcaaa 
      ggcagagac cacagttcg gctttaagc gcctcgcgt 
      aagtccctc gtcgagaca acaaccggg ctagagata 
      cacaatgcg tatagccac cagtag
    mRNA sequence
    • >TC031145 RA
      tgcccggtt tattatgcc atttgcttg cctaattag 
      gtgccatat tttcatcag ccacccggc cgcaatctt 
      cggattcct cagcgtgaa gaaatggct cttgcgaaa 
      gaagcacca gatcgaact ggcccgcaa acgcggctg 
      gaagggcta ctgggtgtg cttgaaggg caccactct 
      gctgttcta cccctgcga ctcgcgcga gggccgctc 
      cgtggaggc ggcgcccaa acacctcat catcgtcga 
      cggcgccat catgcagcc catcccaga acaccccaa 
      aagagacta cattttctg cctgagtac ggcgttcgg 
      tgacgccta cttgttcca agcgccttg tcaggtcga 
      gctggagaa ctgggtgaa tagcattca cagcgcctg 
      tgctgcggc ttttgctcg ccatcgcgg caaaaccgg 
      cactctgca tctgctgca agaggagat tttccgctt 
      ggagaaagc catcgagac cgactacaa gctcaaaca 
      catggccga aatgcagca aagcgtcgt gggagactc 
      cgacgctcg gcagcaaat cgccaatca ggtggtaca 
      atgggagga aaacctgga aaggctgca ttgcgaaca 
      gtttcggct ccggtgcta catggccag tctgcagag 
      cggagaact gcccaaccc taagtcctt gctgacgca 
      cgtctcgag agcaacgaa gaatacctt aaataagtt 
      gggagtgtt taccgtttc ctcctttca tgcctttat 
      ttgtgcgcg gagtccctc tttactcaa caacctgct 
      cgcaggccg aggagcgac gaagagaag gccgccgct 
      tctgtccag gtccaacag cggctcgag caggaggtc 
      tttgcagat aaattcccg ggaagagag cgaaaagac 
      cgttaaggt gtcactgcc agacgggaa ttcggccac 
      ggtctacgt gcgcgactc catgtcggt ggaggagtt 
      cctggcgag tgcttgcgc gaggaaaaa cctcagctg 
      cacggaaca cttcataag ggtgaagaa gaggcggga 
      catggagga ccacaacta ctttgtgcc tcacagaag 
      cgacctgat cgagactta cctccacac gcacgaagt 
      cgtcgaggt ctgtgcgaa aatcctgta ccaagtgga 
      gctctcgcg gagcacgtt ggagcagat gtggggctt 
      cagcgtcga ggccgaact ggtcgaaaa cgccgacag 
      acaggacga gctttgctg ctacgtcag tcgcgtcga 
      agataaaag cgtagcgat gcaaaacgg catcataaa 
      gggcgacga aatcatggt cataaacgg cgccatcgt 
      gtccgacct ggacatgat gtacctcga aagtgtctt 
      gcaggagga gctggcttt gtgtatgat gatgaggtc 
      gtcccggac cgagcctcc ggaactggt tggaatcct 
      ccgggcgac tgacgacat catcgaaag cttagtctg 
      tcctccacc tccgtcgga acccccagt catcagcga 
      ggaaatgat ctccggact catcgtgcc tgctccagg 
      ttggagcaa agaccgcta ctctaccga gtctgaaaa 
      tccgccttc gtcgatgac cgattccgg gataaaggt 
      gagttcgcg tacaaactc gttcgaaat cgaaaacct 
      gctgaaaac tgctgaaca agtgaccgg tttctgtcg 
      ctcacctgt tgaaacgcg caaatcgag tcctacagg 
      aagtttagt cagcaacac ttcccagac tctactaac 
      tccctctcg gcagctcag cgacgccga gaagttgcg 
      aaaagtcat cttggagtt ggtagatac ggagcgagc 
      gtacgtcaa gcatctcaa caatttatt ggagaacta 
      cttggagcc gctgaagcg ggagacgtt cctgtccaa 
      cgcggaaat caacgcttt gttcggaaa catccagga 
      gatcgtcac gttccagag acagttcct tcagaacct 
      cgaagaggc tctcgatct tgagccgga tttccataa 
      atttgaata cagcagcca atttaaaaa tgtattgtt 
      ttccatcgg gagcgcatt tttgtatta tgtaaatca 
      cttcaaatt atacagctc gttctgcgc gagccacag 
      caaagcgca gaaagtttt gcatccgaa cgaaggcaa 
      tcaagctct gcaggaatt cctcgcggc ccgtaatcc 
      caaacagca acattcctc cactctaga gtcttatct 
      cataaaacc gattcagcg gattctcaa gtaccctct 
      gctcttgca acagttgcg gaacttgac cgaccccaa 
      caccgacga acaccaaca cttagtcga ggccctcaa 
      aggaatgga gaaagtggc cgaacacat caacgagat 
      gcagcgaat ccacgagga gtacggggc tattttcga 
      ccacctctt ccgccaaca ccaaaagag ttcgaagca 
      gagtatcga tctaagtcc gggagattt attatatta 
      tggaggagt ggaatggct gaacatatc ggatttcct 
      ggggaaaat caagaaagg gcttgaact acacgcgat 
      gtgcttcgt gtttaaaac cgcggtcgt ttttctctg 
      caaggaacg tcttcgcca gaaaaagaa actaatggg 
      cgtttccag taagggttc ctcatccga agtcgaaat 
      tattcgcta tcaagtgct cattcctgt aaccgaagt 
      tcaagtacg agcttcctc cgctaagga tatggattc 
      tcatttcct gtgggaact gattcactt aagaagtca 
      attacagag gagatcaga gaaggtcta cgttttgtc 
      taatagcac tgctgactt cagaaacgc ctttcttaa 
      aaccatcag acaaattat cagggaatc ggttaggaa 
      tatgtcaat accggcgac gaaaccggg cacgggacc 
      gggaatcct cctcgtcac aggacacaa aggggagaa 
      tctcaacag tcaaacact ggagcggcc cgcaacgaa 
      aacagtcat agtgcaagg atcacacac tttgggcaa 
      aacgaaaaa gaagtcctc gcaaaggca ctcggccgg 
      gaatataga ctacgacaa catcagcca ggaagggga 
      cgaacccac cacgacctt caggtcccg gagtaaaac 
      agtaggaga tgccgcaga tcctcccaa acgcagtga 
      ccacgaccg gaacgacac cggagccaa atccgaggg 
      cgaggagga ctcccaagg gggcaagaa ggccactct 
      cggccggac ccccaacca cctaactct cagcacgac 
      ctcgacttt gtctgccgg cagtaccgg aagccaagc 
      ccgcttgat ccaatcgtc gcacgcccc ggaaacctt 
      ccaacccat gcagaccga agaactagg gtcgcctgt 
      gtggaagcc aagagacag tagtttaga ggcaacctc 
      aaccaccct cccgcgcaa aggcagaga ccacagttc 
      ggctttaag cgcctcgcg taagtccct cgtcgagac 
      aacaaccgg gctagagat acacaatgc gtatagcca 
      ccagtagaa aaatattta tatcacctg tttgtatga 
      aggggccga gccccgcca ctgtgctgt tcatgcaaa 
      cggacacac gaatcgaat tgttaaacg ctgtacaag 
      ttatttaaa taattgtta aatatttgt gactttacc 
      gactgagaa gtttttttc gcccctcac tcactcccg 
      aggtattgg ttgggattt tgttcgata aatatattc 
      caagttgtt gtaccaaga ctcagtatt acaggggaa 
      aatcattcg atatacttt tagaacgtt tatgcatat 
      ttgcaagat ctttataga ggtaatttt tattgcata 
      ggttcgttg tttgatgag aaaatctat caaaatgcg 
      cccgccggg aacgcggct tcttcgcgg gcacactaa 
      tttataatt ttagatctc aaaaacata ttcctatta 
      atactaata ttacgattt gttgaaaga aatttcaga 
      ttttattta ctaatttaa agaattttt aaataaaaa 
      ccttaatat gtaatttaa tgcaatcaa gtgaatcag 
      tagagaggg actagaaca ttgtcacat tattattga 
      atatataaa aatctagaa atagttgat ccgtacgta 
      ggtttaatg aacaaatag ctatgtatt tttaaagaa 
      tacatatta gtttaataa tttaatatt aacttatgt 
      aatagacgc gtccggcgg cggattctt tcccccgaa 
      acgacccgc cgccggccc tagaaatat ttacaataa 
      attaaccga tcacattgt caaattctg ctgtcaaca 
      aaatgttta atcgcacaa gacaaccaa aaacaatat 
      aattttcca aacttggtg cccgtttaa tttcggttt 
      cccaccacc aagttgctc aaatgtaat cctagtcgt 
      acgttatta cttttgtat taattttta ttgtgtaag 
      tgttccatc gcgactcag ttccaccct caccccctt 
      ttttttact agcgagaca attagtaca agcgagcgg 
      tgaagtttg tcaaaaaaa cgcatcagt gtttttctg 
      aaaagtgcc aaaatcaaa agaaacgaa tttaaatca 
      t
    • >TC031145 RB
      tgcccggtt tattatgcc atttgcttg cctaattag 
      gtgccatat tttcatcag ccacccggc cgcaatctt 
      cggattcct cagcgtgaa gaaatggct cttgcgaaa 
      gaagcacca gatcgaact ggcccgcaa acgcggctg 
      gaagggcta ctgggtgtg cttgaaggg caccactct 
      gctgttcta cccctgcga ctcgcgcga gggccgctc 
      cgtggaggc ggcgcccaa acacctcat catcgtcga 
      cggcgccat catgcagcc catcccaga acaccccaa 
      aagagacta cattttctg cctgagtac ggcgttcgg 
      tgacgccta cttgttcca agcgccttg tcaggtcga 
      gctggagaa ctgggtgaa tagcattca cagcgcctg 
      tgctgcggc ttttgctcg ccatcgcgg caaaaccgg 
      cactctgca tctgctgca agaggagat tttccgctt 
      ggagaaagc catcgagac cgactacaa gctcaaaca 
      catggccga aatgcagca aagcgtcgt gggagactc 
      cgacgctcg gcagcaaat cgccaatca ggtggtaca 
      atgggagga aaacctgga aaggctgca ttgcgaaca 
      gtttcggct ccggtgcta catggccag tctgcagag 
      cggagaact gcccaaccc taagtcctt gctgacgca 
      cgtctcgag agcaacgaa gaatacctt aaataagtt 
      gggagtgtt taccgtttc ctcctttca tgcctttat 
      ttgtgcgcg gagtccctc tttactcaa caacctgct 
      cgcaggccg aggagcgac gaagagaag gccgccgct 
      tctgtccag gtccaacag cggctcgag caggaggtc 
      tttgcagat aaattcccg ggaagagag cgaaaagac 
      cgttaaggt gtcactgcc agacgggaa ttcggccac 
      ggtctacgt gcgcgactc catgtcggt ggaggagtt 
      cctggcgag tgcttgcgc gaggaaaaa cctcagctg 
      cacggaaca cttcataag ggtgaagaa gaggcggga 
      catggagga ccacaacta ctttgtgcc tcacagaag 
      cgacctgat cgagactta cctccacac gcacgaagt 
      cgtcgaggt ctgtgcgaa aatcctgta ccaagtgga 
      gctctcgcg gagcacgtt ggagcagat gtggggctt 
      cagcgtcga ggccgaact ggtcgaaaa cgccgacag 
      acaggacga gctttgctg ctacgtcag tcgcgtcga 
      agataaaag cgtagcgat gcaaaacgg catcataaa 
      gggcgacga aatcatggt cataaacgg cgccatcgt 
      gtccgacct ggacatgat gtacctcga aagtgtctt 
      gcaggagga gctggcttt gtgtatgat gatgaggtc 
      gtcccggac cgagcctcc ggaactggt tggaatcct 
      ccgggcgac tgacgacat catcgaaag cttagtctg 
      tcctccacc tccgtcgga acccccagt catcagcga 
      ggaaatgat ctccggact catcgtgcc tgctccagg 
      caaagaccg ctactctac cgagtctga aaatccgcc 
      ttcgtcgat gaccgattc cgggataaa ggtgagttc 
      gcgtacaaa ctcgttcga aatcgaaaa cctgctgaa 
      aactgctga acaagtgac cggtttctg tcgctcacc 
      tgttgaaac gcgcaaatc gagtcctac aggaagttt 
      agtcagcaa cacttccca gactctact aactccctc 
      tcggcagct cagcgacgc cgagaagtt gcgaaaagt 
      catcttgga gttggtaga tacggagcg agcgtacgt 
      caagcatct caacaattt attggagaa ctacttgga 
      gccgctgaa gcgggagac gttcctgtc caacgcgga 
      aatcaacgc tttgttcgg aaacatcca ggagatcgt 
      cacgttcca gagacagtt ccttcagaa cctcgaaga 
      ggctctcga tcttgagcc ggatttcca taaatttga 
      atacagcag ccaatttaa aaatgtatt gttttccat 
      cgggagcgc atttttgta ttatgtaaa tcacttcaa 
      attatacag ctcgttctg cgcgagcca cagcaaagc 
      gcagaaagt tttgcatcc gaacgaagg caatcaagc 
      tctgcagga attcctcgc ggcccgtaa tcccaaaca 
      gcaacattc ctccactct agagtctta tctcataaa 
      accgattca gcggattct caagtaccc tctgctctt 
      gcaacagtt gcggaactt gaccgaccc caacaccga 
      cgaacacca acacttagt cgaggccct caaaggaat 
      ggagaaagt ggccgaaca catcaacga gatgcagcg 
      aatccacga ggagtacgg ggctatttt cgaccacct 
      cttccgcca acaccaaaa gagttcgaa gcagagtat 
      cgatctaag tccgggaga tttattata ttatggagg 
      agtggaatg gctgaacat atcggattt cctggggaa 
      aatcaagaa agggcttga actacacgc gatgtgctt 
      cgtgtttaa aaccgcggt cgtttttct ctgcaagga 
      acgtcttcg ccagaaaaa gaaactaat gggcgtttc 
      cagtaaggg ttcctcatc cgaagtcga aattattcg 
      ctatcaagt gctcattcc tgtaaccga agttcaagt 
      acgagcttc ctccgctaa ggatatgga ttctcattt 
      cctgtggga actgattca cttaagaag tcaattaca 
      gaggagatc agagaaggt ctacgtttt gtctaatag 
      cactgctga cttcagaaa cgcctttct taaaaccat 
      cagacaaat tatcaggga atcggttag gaatatgtc 
      aataccggc gacgaaacc gggcacggg accgggaat 
      cctcctcgt cacaggaca caaagggga gaatctcaa 
      cagtcaaac actggagcg gcccgcaac gaaaacagt 
      catagtgca aggatcaca cactttggg caaaacgaa 
      aaagaagtc ctcgcaaag gcactcggc cgggaatat 
      agactacga caacatcag ccaggaagg ggacgaacc 
      caccacgac cttcaggtc ccggagtaa aacagtagg 
      agatgccgc agatcctcc caaacgcag tgaccacga 
      ccggaacga caccggagc caaatccga gggcgagga 
      ggactccca agggggcaa gaaggccac tctcggccg 
      gacccccaa ccacctaac tctcagcac gacctcgac 
      tttgtctgc cggcagtac cggaagcca agcccgctt 
      gatccaatc gtcgcacgc cccggaaac cttccaacc 
      catgcagac cgaagaact agggtcgcc tgtgtggaa 
      gccaagaga cagtagttt agaggcaac ctcaaccac 
      cctcccgcg caaaggcag agaccacag ttcggcttt 
      aagcgcctc gcgtaagtc cctcgtcga gacaacaac 
      cgggctaga gatacacaa tgcgtatag ccaccagta 
      gaaaaatat ttatatcac ctgtttgta tgaaggggc 
      cgagccccg ccactgtgc tgttcatgc aaacggaca 
      cacgaatcg aattgttaa acgctgtac aagttattt 
      aaataattg ttaaatatt tgtgacttt accgactga 
      gaagttttt ttcgcccct cactcactc ccgaggtat 
      tggttggga ttttgttcg ataaatata ttccaagtt 
      gttgtacca agactcagt attacaggg gaaaatcat 
      tcgatatac ttttagaac gtttatgca tatttgcaa 
      gatctttat agaggtaat ttttattgc ataggttcg 
      ttgtttgat gagaaaatc tatcaaaat gcgcccgcc 
      gggaacgcg gcttcttcg cgggcacac taatttata 
      attttagat ctcaaaaac atattccta ttaatacta 
      atattacga tttgttgaa agaaatttc agattttat 
      ttactaatt taaagaatt tttaaataa aaaccttaa 
      tatgtaatt taatgcaat caagtgaat cagtagaga 
      gggactaga acattgtca cattattat tgaatatat 
      aaaaatcta gaaatagtt gatccgtac gtaggttta 
      atgaacaaa tagctatgt atttttaaa gaatacata 
      ttagtttaa taatttaat attaactta tgtaataga 
      cgcgtccgg cggcggatt ctttccccc gaaacgacc 
      cgccgccgg ccctagaaa tatttacaa taaattaac 
      cgatcacat tgtcaaatt ctgctgtca acaaaatgt 
      ttaatcgca caagacaac caaaaacaa tataatttt 
      ccaaacttg gtgcccgtt taatttcgg tttcccacc 
      accaagttg ctcaaatgt aatcctagt cgtacgtta 
      ttacttttg tattaattt ttattgtgt aagtgttcc 
      atcgcgact cagttccac cctcacccc ctttttttt 
      actagcgag acaattagt acaagcgag cggtgaagt 
      ttgtcaaaa aaacgcatc agtgttttt ctgaaaagt 
      gccaaaatc aaaagaaac gaatttaaa tcat