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Gene information
General information
TC014300 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8688707..8692791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014300
    iBeetle screen iB_02796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014300 RA
      MDKSQLVKL LCELNGIST DSDEENEGD IFETRYVKA 
      SETIMCSCE EILSKVPSL AVSLEGTEP NNKIKYMLL 
      NFYILELIN EQLGTSDMT VLSVQETKN VKACLQILM 
      NNGILKNIQ PKLPYFIPT HSKSDILLD YTLLKCTVL 
      GFYKDMKYP NLRMLILPH LLQGMLVAI YQLAYCPLK 
      KPTGAQDNK FVMTLETYE KLVHDQEQF RKILDDLSH 
      NIHPTILIR ETMVLFQAN APVWFKTAI ANTLTKIIR 
      SPKGVRNIA SAMLSGIED DTTKTWQIL DVLTKLITS 
      CRKFPDFET NICNQVLNL LIEARDLLV FERLVVSCT 
      KCLYSEDKA LCDAVFVKR ITTPLLYFA NKEHQFRDE 
      EVTDDIKQT VRLLYGCFI DGLPTNLLR PYINVLFHL 
      YHLTVKSAL ESTRKELRE ILIKFLQAC TRPEMHELL 
      DNFIFGFTL NGILQFRND VVIEIDQKI IVKNSPHVI 
      IYTTADVMD TVLNLLDLE QKIVLFGFL LNCVTDKGK 
      YFPTLRNST ELLGNEDHV TGTVLERLL TIHRILSQL 
      AENKELQQF LRNKPDDII NYINNVLAQ ESDEQLIFL 
      VLMILDNLI ANSSAGAFP KYKILEHSL STLAESNNQ 
      ELKTLCHKI KRAINSETH SAEATDEKT NFEKALEDV 
      CDPLLPVRG HGLLTLSKL IEKKDKQAV QRKQYLLNL 
      FQLHLKDDD SFIYLNAVQ GLAALADTF TDTVLETLC 
      EEYSDFTKK GEKAQEIRM KLGEVLVRV TKILGEMAP 
      KYKAVLLNT FLSGTKDDD HLIRASSLS NLGEVCRVL 
      NYKLGTIVT EVLVCVHAI VATDKAVEA RRAAVTVIR 
      QLFVGLQSE MISFLKEEI LPIYRTLKE IYYHDKDDV 
      VRLQAQLAL EELNENMKS FVFPNARLN SEKKVIMLN
    CDS sequence
    • >TC014300 RA
      atggataaa tcacagttg gtaaaatta ttgtgtgaa 
      ctcaacgga atttccaca gactcagat gaagagaac 
      gagggtgac atatttgaa acaagatac gtaaaagct 
      tcagaaacc attatgtgt tcttgtgag gaaatattg 
      tcaaaagtt ccatcactt gcagtttcg ttagaagga 
      acggagccc aataataaa ataaaatac atgttgtta 
      aatttttat attttggaa ttaataaac gaacagctc 
      ggaacatcc gacatgact gttttgagt gtccaagaa 
      acaaaaaat gtaaaggcg tgcttacaa atccttatg 
      aataatggg attctaaaa aacattcaa ccgaaactc 
      ccttatttt attccaacg cacagtaaa agtgacatt 
      ttacttgac tacacttta ttaaaatgc accgtactt 
      gggttttac aaggacatg aaatacccg aatttaaga 
      atgttgatt ttaccacat ttgctccaa ggaatgttg 
      gttgcaatt taccagctc gcttactgt cctctgaaa 
      aaaccaact ggcgctcag gataataag ttcgtaatg 
      actctcgaa acgtatgaa aaactggtc cacgaccaa 
      gaacaattt cgaaaaata ttagacgat ttgagtcac 
      aatattcat cccacaatc ctgatccgg gaaacaatg 
      gtcttattc caagccaat gctcccgtg tggttcaaa 
      acagcgatt gcaaacact ttgactaag ataattcga 
      tcgccgaaa ggcgtccga aacatcgct tctgccatg 
      ctttccggc atcgaagac gacaccaca aaaacatgg 
      caaattctg gacgtgcta acgaagctt ataacgagc 
      tgtcgtaaa ttcccagac tttgagaca aacatttgc 
      aatcaagtg ttaaatctc ctgatcgag gcccgtgat 
      ttgttggtt ttcgaacgg ttggttgtg tcttgcacg 
      aaatgtctc tattccgag gataaagca ctttgcgat 
      gcagttttt gtcaagagg ataacgact ccgttatta 
      tatttcgcc aacaaagaa caccaattt cgtgacgag 
      gaggtaacg gatgacatt aaacaaact gtgcgtctg 
      ctttacgga tgcttcatc gacggctta ccgaccaat 
      ctgttgagg ccttacatc aacgtcttg ttccatttg 
      tatcactta actgtgaaa tcggcgcta gaatcgacc 
      agaaaggag ctgagagag attttaatt aagtttttg 
      caagcctgc accaggcca gagatgcac gaattattg 
      gacaacttt attttcggg tttacgctt aacgggatt 
      ttacagttc cggaatgac gtcgtcatt gagattgat 
      caaaagatt atcgtaaaa aacagtcca catgtcatt 
      atttacacg actgcagat gttatggat acagttttg 
      aatctcctc gatctcgag caaaaaatc gtcttgttc 
      ggatttttg ctaaactgt gttaccgac aaagggaaa 
      tatttcccg actcttcgt aacagtaca gaattatta 
      ggtaatgaa gaccatgta acgggtacc gttcttgaa 
      cgcctttta acaatccat agaatccta tcccaatta 
      gctgaaaac aaagaactg caacagttt ctcaggaac 
      aaacctgac gatatcatc aattacatt aacaacgtg 
      ctggctcaa gagagtgac gagcagctc attttcctg 
      gttctgatg attctggac aatttaatc gcaaattca 
      tcagcgggt gctttccca aaatacaag attttggaa 
      cactcctta tcaacacta gctgagtca aacaaccag 
      gaattgaaa acactgtgt cacaaaatc aaacgcgca 
      ataaacagc gaaacccat tcggctgaa gccacggac 
      gagaaaacc aattttgag aaagcgcta gaggacgtt 
      tgtgaccct ttgctccca gttagaggg cacggtcta 
      ttaactcta tcaaagttg attgaaaag aaggacaaa 
      caagcggtt cagagaaaa cagtatttg cttaattta 
      ttccagttg cacttaaaa gacgacgat tcgttcatt 
      tatttgaac gccgtccag ggccttgcg gccctagct 
      gacactttc acagacaca gttctggag actctatgc 
      gaggagtac tccgatttt acgaagaaa ggggaaaag 
      gcccaagaa attcggatg aagctgggc gaagtcttg 
      gttcgcgtc acaaaaatt ttgggcgaa atggccccg 
      aaatacaag gccgtgctc ttgaacact ttcctgtcg 
      gggacaaag gacgacgac cacctgatc cgagcgtcc 
      agcttatcg aatttaggg gaagtgtgt agagtgctt 
      aattacaag ctggggacg atagtaact gaggttttg 
      gtgtgtgta cacgcaatt gtggcgaca gataaggcc 
      gtagaggca cgacgtgcc gccgttacg gtcataaga 
      cagttgttt gttggactt caaagcgaa atgatatcg 
      tttctcaaa gaagaaatt ttgccgatt tacaggact 
      ctcaaagag atctattac cacgataag gacgacgtt 
      gtgcggctg caagcgcag ctagcgctt gaggaactc 
      aacgaaaat atgaaaagt ttcgtgttc cccaacgcc 
      cggcttaat tcggagaag aaggttatt atgttgaac 
      taa
    mRNA sequence
    • >TC014300 RA
      tggtattac caattactg aaatcttta ttttaatat 
      agatcagtt tgggaagtg gtttaaaag tgttaacat 
      gtaccctaa tgctgtgca tattgtaac ctaaataaa 
      agtgatttt ttgtaacga ttttttatt tctttcgct 
      tgtccaaca taattgcat aatggataa atcacagtt 
      ggtaaaatt attgtgtga actcaacgg aatttccac 
      agactcaga tgaagagaa cgagggtga catatttga 
      aacaagata cgtaaaagc ttcagaaac cattatgtg 
      ttcttgtga ggaaatatt gtcaaaagt tccatcact 
      tgcagtttc gttagaagg aacggagcc caataataa 
      aataaaata catgttgtt aaattttta tattttgga 
      attaataaa cgaacagct cggaacatc cgacatgac 
      tgttttgag tgtccaaga aacaaaaaa tgtaaaggc 
      gtgcttaca aatccttat gaataatgg gattctaaa 
      aaacattca accgaaact cccttattt tattccaac 
      gcacagtaa aagtgacat tttacttga ctacacttt 
      attaaaatg caccgtact tgggtttta caaggacat 
      gaaataccc gaatttaag aatgttgat tttaccaca 
      tttgctcca aggaatgtt ggttgcaat ttaccagct 
      cgcttactg tcctctgaa aaaaccaac tggcgctca 
      ggataataa gttcgtaat gactctcga aacgtatga 
      aaaactggt ccacgacca agaacaatt tcgaaaaat 
      attagacga tttgagtca caatattca tcccacaat 
      cctgatccg ggaaacaat ggtcttatt ccaagccaa 
      tgctcccgt gtggttcaa aacagcgat tgcaaacac 
      tttgactaa gataattcg atcgccgaa aggcgtccg 
      aaacatcgc ttctgccat gctttccgg catcgaaga 
      cgacaccac aaaaacatg gcaaattct ggacgtgct 
      aacgaagct tataacgag ctgtcgtaa attcccaga 
      ctttgagac aaacatttg caatcaagt gttaaatct 
      cctgatcga ggcccgtga tttgttggt tttcgaacg 
      gttggttgt gtcttgcac gaaatgtct ctattccga 
      ggataaagc actttgcga tgcagtttt tgtcaagag 
      gataacgac tccgttatt atatttcgc caacaaaga 
      acaccaatt tcgtgacga ggaggtaac ggatgacat 
      taaacaaac tgtgcgtct gctttacgg atgcttcat 
      cgacggctt accgaccaa tctgttgag gccttacat 
      caacgtctt gttccattt gtatcactt aactgtgaa 
      atcggcgct agaatcgac cagaaagga gctgagaga 
      gattttaat taagttttt gcaagcctg caccaggcc 
      agagatgca cgaattatt ggacaactt tattttcgg 
      gtttacgct taacgggat tttacagtt ccggaatga 
      cgtcgtcat tgagattga tcaaaagat tatcgtaaa 
      aaacagtcc acatgtcat tatttacac gactgcaga 
      tgttatgga tacagtttt gaatctcct cgatctcga 
      gcaaaaaat cgtcttgtt cggattttt gctaaactg 
      tgttaccga caaagggaa atatttccc gactcttcg 
      taacagtac agaattatt aggtaatga agaccatgt 
      aacgggtac cgttcttga acgcctttt aacaatcca 
      tagaatcct atcccaatt agctgaaaa caaagaact 
      gcaacagtt tctcaggaa caaacctga cgatatcat 
      caattacat taacaacgt gctggctca agagagtga 
      cgagcagct cattttcct ggttctgat gattctgga 
      caatttaat cgcaaattc atcagcggg tgctttccc 
      aaaatacaa gattttgga acactcctt atcaacact 
      agctgagtc aaacaacca ggaattgaa aacactgtg 
      tcacaaaat caaacgcgc aataaacag cgaaaccca 
      ttcggctga agccacgga cgagaaaac caattttga 
      gaaagcgct agaggacgt ttgtgaccc tttgctccc 
      agttagagg gcacggtct attaactct atcaaagtt 
      gattgaaaa gaaggacaa acaagcggt tcagagaaa 
      acagtattt gcttaattt attccagtt gcacttaaa 
      agacgacga ttcgttcat ttatttgaa cgccgtcca 
      gggccttgc ggccctagc tgacacttt cacagacac 
      agttctgga gactctatg cgaggagta ctccgattt 
      tacgaagaa aggggaaaa ggcccaaga aattcggat 
      gaagctggg cgaagtctt ggttcgcgt cacaaaaat 
      tttgggcga aatggcccc gaaatacaa ggccgtgct 
      cttgaacac tttcctgtc ggggacaaa ggacgacga 
      ccacctgat ccgagcgtc cagcttatc gaatttagg 
      ggaagtgtg tagagtgct taattacaa gctggggac 
      gatagtaac tgaggtttt ggtgtgtgt acacgcaat 
      tgtggcgac agataaggc cgtagaggc acgacgtgc 
      cgccgttac ggtcataag acagttgtt tgttggact 
      tcaaagcga aatgatatc gtttctcaa agaagaaat 
      tttgccgat ttacaggac tctcaaaga gatctatta 
      ccacgataa ggacgacgt tgtgcggct gcaagcgca 
      gctagcgct tgaggaact caacgaaaa tatgaaaag 
      tttcgtgtt ccccaacgc ccggcttaa ttcggagaa 
      gaaggttat tatgttgaa ctaaatgaa aacgcttgt 
      ttttgtaaa agtttattc gtagtataa ggaatagaa 
      aaataaata gccaattgc atactatac acggtgttt 
      ttgttcctg gttatcccc tttgtaaca caacaatca 
      cgcaaacac ctagtatat ggggagaat acgcaattg 
      aattaacaa atacccaaa aatag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY