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Gene information
General information
TC014300 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8688707..8692791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014300
    iBeetle screen iB_02796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014300 RA
      MDKSQLVKL LCELNGIST DSDEENEGD IFETRYVKA 
SETIMCSCE EILSKVPSL AVSLEGTEP NNKIKYMLL 
NFYILELIN EQLGTSDMT VLSVQETKN VKACLQILM 
NNGILKNIQ PKLPYFIPT HSKSDILLD YTLLKCTVL 
GFYKDMKYP NLRMLILPH LLQGMLVAI YQLAYCPLK 
KPTGAQDNK FVMTLETYE KLVHDQEQF RKILDDLSH 
NIHPTILIR ETMVLFQAN APVWFKTAI ANTLTKIIR 
SPKGVRNIA SAMLSGIED DTTKTWQIL DVLTKLITS 
CRKFPDFET NICNQVLNL LIEARDLLV FERLVVSCT 
KCLYSEDKA LCDAVFVKR ITTPLLYFA NKEHQFRDE 
EVTDDIKQT VRLLYGCFI DGLPTNLLR PYINVLFHL 
YHLTVKSAL ESTRKELRE ILIKFLQAC TRPEMHELL 
DNFIFGFTL NGILQFRND VVIEIDQKI IVKNSPHVI 
IYTTADVMD TVLNLLDLE QKIVLFGFL LNCVTDKGK 
YFPTLRNST ELLGNEDHV TGTVLERLL TIHRILSQL 
AENKELQQF LRNKPDDII NYINNVLAQ ESDEQLIFL 
VLMILDNLI ANSSAGAFP KYKILEHSL STLAESNNQ 
ELKTLCHKI KRAINSETH SAEATDEKT NFEKALEDV 
CDPLLPVRG HGLLTLSKL IEKKDKQAV QRKQYLLNL 
FQLHLKDDD SFIYLNAVQ GLAALADTF TDTVLETLC 
EEYSDFTKK GEKAQEIRM KLGEVLVRV TKILGEMAP 
KYKAVLLNT FLSGTKDDD HLIRASSLS NLGEVCRVL 
NYKLGTIVT EVLVCVHAI VATDKAVEA RRAAVTVIR 
QLFVGLQSE MISFLKEEI LPIYRTLKE IYYHDKDDV 
VRLQAQLAL EELNENMKS FVFPNARLN SEKKVIMLN
    CDS sequence
    • >TC014300 RA
      atggataaa tcacagttg gtaaaatta ttgtgtgaa 
ctcaacgga atttccaca gactcagat gaagagaac 
gagggtgac atatttgaa acaagatac gtaaaagct 
tcagaaacc attatgtgt tcttgtgag gaaatattg 
tcaaaagtt ccatcactt gcagtttcg ttagaagga 
acggagccc aataataaa ataaaatac atgttgtta 
aatttttat attttggaa ttaataaac gaacagctc 
ggaacatcc gacatgact gttttgagt gtccaagaa 
acaaaaaat gtaaaggcg tgcttacaa atccttatg 
aataatggg attctaaaa aacattcaa ccgaaactc 
ccttatttt attccaacg cacagtaaa agtgacatt 
ttacttgac tacacttta ttaaaatgc accgtactt 
gggttttac aaggacatg aaatacccg aatttaaga 
atgttgatt ttaccacat ttgctccaa ggaatgttg 
gttgcaatt taccagctc gcttactgt cctctgaaa 
aaaccaact ggcgctcag gataataag ttcgtaatg 
actctcgaa acgtatgaa aaactggtc cacgaccaa 
gaacaattt cgaaaaata ttagacgat ttgagtcac 
aatattcat cccacaatc ctgatccgg gaaacaatg 
gtcttattc caagccaat gctcccgtg tggttcaaa 
acagcgatt gcaaacact ttgactaag ataattcga 
tcgccgaaa ggcgtccga aacatcgct tctgccatg 
ctttccggc atcgaagac gacaccaca aaaacatgg 
caaattctg gacgtgcta acgaagctt ataacgagc 
tgtcgtaaa ttcccagac tttgagaca aacatttgc 
aatcaagtg ttaaatctc ctgatcgag gcccgtgat 
ttgttggtt ttcgaacgg ttggttgtg tcttgcacg 
aaatgtctc tattccgag gataaagca ctttgcgat 
gcagttttt gtcaagagg ataacgact ccgttatta 
tatttcgcc aacaaagaa caccaattt cgtgacgag 
gaggtaacg gatgacatt aaacaaact gtgcgtctg 
ctttacgga tgcttcatc gacggctta ccgaccaat 
ctgttgagg ccttacatc aacgtcttg ttccatttg 
tatcactta actgtgaaa tcggcgcta gaatcgacc 
agaaaggag ctgagagag attttaatt aagtttttg 
caagcctgc accaggcca gagatgcac gaattattg 
gacaacttt attttcggg tttacgctt aacgggatt 
ttacagttc cggaatgac gtcgtcatt gagattgat 
caaaagatt atcgtaaaa aacagtcca catgtcatt 
atttacacg actgcagat gttatggat acagttttg 
aatctcctc gatctcgag caaaaaatc gtcttgttc 
ggatttttg ctaaactgt gttaccgac aaagggaaa 
tatttcccg actcttcgt aacagtaca gaattatta 
ggtaatgaa gaccatgta acgggtacc gttcttgaa 
cgcctttta acaatccat agaatccta tcccaatta 
gctgaaaac aaagaactg caacagttt ctcaggaac 
aaacctgac gatatcatc aattacatt aacaacgtg 
ctggctcaa gagagtgac gagcagctc attttcctg 
gttctgatg attctggac aatttaatc gcaaattca 
tcagcgggt gctttccca aaatacaag attttggaa 
cactcctta tcaacacta gctgagtca aacaaccag 
gaattgaaa acactgtgt cacaaaatc aaacgcgca 
ataaacagc gaaacccat tcggctgaa gccacggac 
gagaaaacc aattttgag aaagcgcta gaggacgtt 
tgtgaccct ttgctccca gttagaggg cacggtcta 
ttaactcta tcaaagttg attgaaaag aaggacaaa 
caagcggtt cagagaaaa cagtatttg cttaattta 
ttccagttg cacttaaaa gacgacgat tcgttcatt 
tatttgaac gccgtccag ggccttgcg gccctagct 
gacactttc acagacaca gttctggag actctatgc 
gaggagtac tccgatttt acgaagaaa ggggaaaag 
gcccaagaa attcggatg aagctgggc gaagtcttg 
gttcgcgtc acaaaaatt ttgggcgaa atggccccg 
aaatacaag gccgtgctc ttgaacact ttcctgtcg 
gggacaaag gacgacgac cacctgatc cgagcgtcc 
agcttatcg aatttaggg gaagtgtgt agagtgctt 
aattacaag ctggggacg atagtaact gaggttttg 
gtgtgtgta cacgcaatt gtggcgaca gataaggcc 
gtagaggca cgacgtgcc gccgttacg gtcataaga 
cagttgttt gttggactt caaagcgaa atgatatcg 
tttctcaaa gaagaaatt ttgccgatt tacaggact 
ctcaaagag atctattac cacgataag gacgacgtt 
gtgcggctg caagcgcag ctagcgctt gaggaactc 
aacgaaaat atgaaaagt ttcgtgttc cccaacgcc 
cggcttaat tcggagaag aaggttatt atgttgaac 
taa
    mRNA sequence
    • >TC014300 RA
      tggtattac caattactg aaatcttta ttttaatat 
agatcagtt tgggaagtg gtttaaaag tgttaacat 
gtaccctaa tgctgtgca tattgtaac ctaaataaa 
agtgatttt ttgtaacga ttttttatt tctttcgct 
tgtccaaca taattgcat aatggataa atcacagtt 
ggtaaaatt attgtgtga actcaacgg aatttccac 
agactcaga tgaagagaa cgagggtga catatttga 
aacaagata cgtaaaagc ttcagaaac cattatgtg 
ttcttgtga ggaaatatt gtcaaaagt tccatcact 
tgcagtttc gttagaagg aacggagcc caataataa 
aataaaata catgttgtt aaattttta tattttgga 
attaataaa cgaacagct cggaacatc cgacatgac 
tgttttgag tgtccaaga aacaaaaaa tgtaaaggc 
gtgcttaca aatccttat gaataatgg gattctaaa 
aaacattca accgaaact cccttattt tattccaac 
gcacagtaa aagtgacat tttacttga ctacacttt 
attaaaatg caccgtact tgggtttta caaggacat 
gaaataccc gaatttaag aatgttgat tttaccaca 
tttgctcca aggaatgtt ggttgcaat ttaccagct 
cgcttactg tcctctgaa aaaaccaac tggcgctca 
ggataataa gttcgtaat gactctcga aacgtatga 
aaaactggt ccacgacca agaacaatt tcgaaaaat 
attagacga tttgagtca caatattca tcccacaat 
cctgatccg ggaaacaat ggtcttatt ccaagccaa 
tgctcccgt gtggttcaa aacagcgat tgcaaacac 
tttgactaa gataattcg atcgccgaa aggcgtccg 
aaacatcgc ttctgccat gctttccgg catcgaaga 
cgacaccac aaaaacatg gcaaattct ggacgtgct 
aacgaagct tataacgag ctgtcgtaa attcccaga 
ctttgagac aaacatttg caatcaagt gttaaatct 
cctgatcga ggcccgtga tttgttggt tttcgaacg 
gttggttgt gtcttgcac gaaatgtct ctattccga 
ggataaagc actttgcga tgcagtttt tgtcaagag 
gataacgac tccgttatt atatttcgc caacaaaga 
acaccaatt tcgtgacga ggaggtaac ggatgacat 
taaacaaac tgtgcgtct gctttacgg atgcttcat 
cgacggctt accgaccaa tctgttgag gccttacat 
caacgtctt gttccattt gtatcactt aactgtgaa 
atcggcgct agaatcgac cagaaagga gctgagaga 
gattttaat taagttttt gcaagcctg caccaggcc 
agagatgca cgaattatt ggacaactt tattttcgg 
gtttacgct taacgggat tttacagtt ccggaatga 
cgtcgtcat tgagattga tcaaaagat tatcgtaaa 
aaacagtcc acatgtcat tatttacac gactgcaga 
tgttatgga tacagtttt gaatctcct cgatctcga 
gcaaaaaat cgtcttgtt cggattttt gctaaactg 
tgttaccga caaagggaa atatttccc gactcttcg 
taacagtac agaattatt aggtaatga agaccatgt 
aacgggtac cgttcttga acgcctttt aacaatcca 
tagaatcct atcccaatt agctgaaaa caaagaact 
gcaacagtt tctcaggaa caaacctga cgatatcat 
caattacat taacaacgt gctggctca agagagtga 
cgagcagct cattttcct ggttctgat gattctgga 
caatttaat cgcaaattc atcagcggg tgctttccc 
aaaatacaa gattttgga acactcctt atcaacact 
agctgagtc aaacaacca ggaattgaa aacactgtg 
tcacaaaat caaacgcgc aataaacag cgaaaccca 
ttcggctga agccacgga cgagaaaac caattttga 
gaaagcgct agaggacgt ttgtgaccc tttgctccc 
agttagagg gcacggtct attaactct atcaaagtt 
gattgaaaa gaaggacaa acaagcggt tcagagaaa 
acagtattt gcttaattt attccagtt gcacttaaa 
agacgacga ttcgttcat ttatttgaa cgccgtcca 
gggccttgc ggccctagc tgacacttt cacagacac 
agttctgga gactctatg cgaggagta ctccgattt 
tacgaagaa aggggaaaa ggcccaaga aattcggat 
gaagctggg cgaagtctt ggttcgcgt cacaaaaat 
tttgggcga aatggcccc gaaatacaa ggccgtgct 
cttgaacac tttcctgtc ggggacaaa ggacgacga 
ccacctgat ccgagcgtc cagcttatc gaatttagg 
ggaagtgtg tagagtgct taattacaa gctggggac 
gatagtaac tgaggtttt ggtgtgtgt acacgcaat 
tgtggcgac agataaggc cgtagaggc acgacgtgc 
cgccgttac ggtcataag acagttgtt tgttggact 
tcaaagcga aatgatatc gtttctcaa agaagaaat 
tttgccgat ttacaggac tctcaaaga gatctatta 
ccacgataa ggacgacgt tgtgcggct gcaagcgca 
gctagcgct tgaggaact caacgaaaa tatgaaaag 
tttcgtgtt ccccaacgc ccggcttaa ttcggagaa 
gaaggttat tatgttgaa ctaaatgaa aacgcttgt 
ttttgtaaa agtttattc gtagtataa ggaatagaa 
aaataaata gccaattgc atactatac acggtgttt 
ttgttcctg gttatcccc tttgtaaca caacaatca 
cgcaaacac ctagtatat ggggagaat acgcaattg 
aattaacaa atacccaaa aatag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY