| General information | |||
| TC014300 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8688707..8692791 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014300 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02796 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC014300 RA
MDKSQLVKL LCELNGIST DSDEENEGD IFETRYVKA SETIMCSCE EILSKVPSL AVSLEGTEP NNKIKYMLL NFYILELIN EQLGTSDMT VLSVQETKN VKACLQILM NNGILKNIQ PKLPYFIPT HSKSDILLD YTLLKCTVL GFYKDMKYP NLRMLILPH LLQGMLVAI YQLAYCPLK KPTGAQDNK FVMTLETYE KLVHDQEQF RKILDDLSH NIHPTILIR ETMVLFQAN APVWFKTAI ANTLTKIIR SPKGVRNIA SAMLSGIED DTTKTWQIL DVLTKLITS CRKFPDFET NICNQVLNL LIEARDLLV FERLVVSCT KCLYSEDKA LCDAVFVKR ITTPLLYFA NKEHQFRDE EVTDDIKQT VRLLYGCFI DGLPTNLLR PYINVLFHL YHLTVKSAL ESTRKELRE ILIKFLQAC TRPEMHELL DNFIFGFTL NGILQFRND VVIEIDQKI IVKNSPHVI IYTTADVMD TVLNLLDLE QKIVLFGFL LNCVTDKGK YFPTLRNST ELLGNEDHV TGTVLERLL TIHRILSQL AENKELQQF LRNKPDDII NYINNVLAQ ESDEQLIFL VLMILDNLI ANSSAGAFP KYKILEHSL STLAESNNQ ELKTLCHKI KRAINSETH SAEATDEKT NFEKALEDV CDPLLPVRG HGLLTLSKL IEKKDKQAV QRKQYLLNL FQLHLKDDD SFIYLNAVQ GLAALADTF TDTVLETLC EEYSDFTKK GEKAQEIRM KLGEVLVRV TKILGEMAP KYKAVLLNT FLSGTKDDD HLIRASSLS NLGEVCRVL NYKLGTIVT EVLVCVHAI VATDKAVEA RRAAVTVIR QLFVGLQSE MISFLKEEI LPIYRTLKE IYYHDKDDV VRLQAQLAL EELNENMKS FVFPNARLN SEKKVIMLN
- >TC014300 RA
atggataaa tcacagttg gtaaaatta ttgtgtgaa ctcaacgga atttccaca gactcagat gaagagaac gagggtgac atatttgaa acaagatac gtaaaagct tcagaaacc attatgtgt tcttgtgag gaaatattg tcaaaagtt ccatcactt gcagtttcg ttagaagga acggagccc aataataaa ataaaatac atgttgtta aatttttat attttggaa ttaataaac gaacagctc ggaacatcc gacatgact gttttgagt gtccaagaa acaaaaaat gtaaaggcg tgcttacaa atccttatg aataatggg attctaaaa aacattcaa ccgaaactc ccttatttt attccaacg cacagtaaa agtgacatt ttacttgac tacacttta ttaaaatgc accgtactt gggttttac aaggacatg aaatacccg aatttaaga atgttgatt ttaccacat ttgctccaa ggaatgttg gttgcaatt taccagctc gcttactgt cctctgaaa aaaccaact ggcgctcag gataataag ttcgtaatg actctcgaa acgtatgaa aaactggtc cacgaccaa gaacaattt cgaaaaata ttagacgat ttgagtcac aatattcat cccacaatc ctgatccgg gaaacaatg gtcttattc caagccaat gctcccgtg tggttcaaa acagcgatt gcaaacact ttgactaag ataattcga tcgccgaaa ggcgtccga aacatcgct tctgccatg ctttccggc atcgaagac gacaccaca aaaacatgg caaattctg gacgtgcta acgaagctt ataacgagc tgtcgtaaa ttcccagac tttgagaca aacatttgc aatcaagtg ttaaatctc ctgatcgag gcccgtgat ttgttggtt ttcgaacgg ttggttgtg tcttgcacg aaatgtctc tattccgag gataaagca ctttgcgat gcagttttt gtcaagagg ataacgact ccgttatta tatttcgcc aacaaagaa caccaattt cgtgacgag gaggtaacg gatgacatt aaacaaact gtgcgtctg ctttacgga tgcttcatc gacggctta ccgaccaat ctgttgagg ccttacatc aacgtcttg ttccatttg tatcactta actgtgaaa tcggcgcta gaatcgacc agaaaggag ctgagagag attttaatt aagtttttg caagcctgc accaggcca gagatgcac gaattattg gacaacttt attttcggg tttacgctt aacgggatt ttacagttc cggaatgac gtcgtcatt gagattgat caaaagatt atcgtaaaa aacagtcca catgtcatt atttacacg actgcagat gttatggat acagttttg aatctcctc gatctcgag caaaaaatc gtcttgttc ggatttttg ctaaactgt gttaccgac aaagggaaa tatttcccg actcttcgt aacagtaca gaattatta ggtaatgaa gaccatgta acgggtacc gttcttgaa cgcctttta acaatccat agaatccta tcccaatta gctgaaaac aaagaactg caacagttt ctcaggaac aaacctgac gatatcatc aattacatt aacaacgtg ctggctcaa gagagtgac gagcagctc attttcctg gttctgatg attctggac aatttaatc gcaaattca tcagcgggt gctttccca aaatacaag attttggaa cactcctta tcaacacta gctgagtca aacaaccag gaattgaaa acactgtgt cacaaaatc aaacgcgca ataaacagc gaaacccat tcggctgaa gccacggac gagaaaacc aattttgag aaagcgcta gaggacgtt tgtgaccct ttgctccca gttagaggg cacggtcta ttaactcta tcaaagttg attgaaaag aaggacaaa caagcggtt cagagaaaa cagtatttg cttaattta ttccagttg cacttaaaa gacgacgat tcgttcatt tatttgaac gccgtccag ggccttgcg gccctagct gacactttc acagacaca gttctggag actctatgc gaggagtac tccgatttt acgaagaaa ggggaaaag gcccaagaa attcggatg aagctgggc gaagtcttg gttcgcgtc acaaaaatt ttgggcgaa atggccccg aaatacaag gccgtgctc ttgaacact ttcctgtcg gggacaaag gacgacgac cacctgatc cgagcgtcc agcttatcg aatttaggg gaagtgtgt agagtgctt aattacaag ctggggacg atagtaact gaggttttg gtgtgtgta cacgcaatt gtggcgaca gataaggcc gtagaggca cgacgtgcc gccgttacg gtcataaga cagttgttt gttggactt caaagcgaa atgatatcg tttctcaaa gaagaaatt ttgccgatt tacaggact ctcaaagag atctattac cacgataag gacgacgtt gtgcggctg caagcgcag ctagcgctt gaggaactc aacgaaaat atgaaaagt ttcgtgttc cccaacgcc cggcttaat tcggagaag aaggttatt atgttgaac taa
- >TC014300 RA
tggtattac caattactg aaatcttta ttttaatat agatcagtt tgggaagtg gtttaaaag tgttaacat gtaccctaa tgctgtgca tattgtaac ctaaataaa agtgatttt ttgtaacga ttttttatt tctttcgct tgtccaaca taattgcat aatggataa atcacagtt ggtaaaatt attgtgtga actcaacgg aatttccac agactcaga tgaagagaa cgagggtga catatttga aacaagata cgtaaaagc ttcagaaac cattatgtg ttcttgtga ggaaatatt gtcaaaagt tccatcact tgcagtttc gttagaagg aacggagcc caataataa aataaaata catgttgtt aaattttta tattttgga attaataaa cgaacagct cggaacatc cgacatgac tgttttgag tgtccaaga aacaaaaaa tgtaaaggc gtgcttaca aatccttat gaataatgg gattctaaa aaacattca accgaaact cccttattt tattccaac gcacagtaa aagtgacat tttacttga ctacacttt attaaaatg caccgtact tgggtttta caaggacat gaaataccc gaatttaag aatgttgat tttaccaca tttgctcca aggaatgtt ggttgcaat ttaccagct cgcttactg tcctctgaa aaaaccaac tggcgctca ggataataa gttcgtaat gactctcga aacgtatga aaaactggt ccacgacca agaacaatt tcgaaaaat attagacga tttgagtca caatattca tcccacaat cctgatccg ggaaacaat ggtcttatt ccaagccaa tgctcccgt gtggttcaa aacagcgat tgcaaacac tttgactaa gataattcg atcgccgaa aggcgtccg aaacatcgc ttctgccat gctttccgg catcgaaga cgacaccac aaaaacatg gcaaattct ggacgtgct aacgaagct tataacgag ctgtcgtaa attcccaga ctttgagac aaacatttg caatcaagt gttaaatct cctgatcga ggcccgtga tttgttggt tttcgaacg gttggttgt gtcttgcac gaaatgtct ctattccga ggataaagc actttgcga tgcagtttt tgtcaagag gataacgac tccgttatt atatttcgc caacaaaga acaccaatt tcgtgacga ggaggtaac ggatgacat taaacaaac tgtgcgtct gctttacgg atgcttcat cgacggctt accgaccaa tctgttgag gccttacat caacgtctt gttccattt gtatcactt aactgtgaa atcggcgct agaatcgac cagaaagga gctgagaga gattttaat taagttttt gcaagcctg caccaggcc agagatgca cgaattatt ggacaactt tattttcgg gtttacgct taacgggat tttacagtt ccggaatga cgtcgtcat tgagattga tcaaaagat tatcgtaaa aaacagtcc acatgtcat tatttacac gactgcaga tgttatgga tacagtttt gaatctcct cgatctcga gcaaaaaat cgtcttgtt cggattttt gctaaactg tgttaccga caaagggaa atatttccc gactcttcg taacagtac agaattatt aggtaatga agaccatgt aacgggtac cgttcttga acgcctttt aacaatcca tagaatcct atcccaatt agctgaaaa caaagaact gcaacagtt tctcaggaa caaacctga cgatatcat caattacat taacaacgt gctggctca agagagtga cgagcagct cattttcct ggttctgat gattctgga caatttaat cgcaaattc atcagcggg tgctttccc aaaatacaa gattttgga acactcctt atcaacact agctgagtc aaacaacca ggaattgaa aacactgtg tcacaaaat caaacgcgc aataaacag cgaaaccca ttcggctga agccacgga cgagaaaac caattttga gaaagcgct agaggacgt ttgtgaccc tttgctccc agttagagg gcacggtct attaactct atcaaagtt gattgaaaa gaaggacaa acaagcggt tcagagaaa acagtattt gcttaattt attccagtt gcacttaaa agacgacga ttcgttcat ttatttgaa cgccgtcca gggccttgc ggccctagc tgacacttt cacagacac agttctgga gactctatg cgaggagta ctccgattt tacgaagaa aggggaaaa ggcccaaga aattcggat gaagctggg cgaagtctt ggttcgcgt cacaaaaat tttgggcga aatggcccc gaaatacaa ggccgtgct cttgaacac tttcctgtc ggggacaaa ggacgacga ccacctgat ccgagcgtc cagcttatc gaatttagg ggaagtgtg tagagtgct taattacaa gctggggac gatagtaac tgaggtttt ggtgtgtgt acacgcaat tgtggcgac agataaggc cgtagaggc acgacgtgc cgccgttac ggtcataag acagttgtt tgttggact tcaaagcga aatgatatc gtttctcaa agaagaaat tttgccgat ttacaggac tctcaaaga gatctatta ccacgataa ggacgacgt tgtgcggct gcaagcgca gctagcgct tgaggaact caacgaaaa tatgaaaag tttcgtgtt ccccaacgc ccggcttaa ttcggagaa gaaggttat tatgttgaa ctaaatgaa aacgcttgt ttttgtaaa agtttattc gtagtataa ggaatagaa aaataaata gccaattgc atactatac acggtgttt ttgttcctg gttatcccc tttgtaaca caacaatca cgcaaacac ctagtatat ggggagaat acgcaattg aattaacaa atacccaaa aatag
