Status
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Gene information
General information
TC010470 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7900735..7906145
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010470
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010470 RA
      MSDPPVMEW SVSDTEVWL RKEGFDDTV IKILCHENQ 
      LNGKCLLVL NEHDFAAEP LSSLTLRDR KCLYIACKT 
      LQRDNQNVL IDLGILEIP NVNLYAPQQ HFNNKHECS 
      EYSESERIS PPVSEDGRI PRLPPEKTK AFVSFCYVV 
      GVTWITAFV MVIVHDRVP DMKKYPPLP DIFLDNVPH 
      IPWAFDMCE ITGTFLFSI WLAVLLFHR HRFILMRRF 
      FALSGTVFL LRCVTMLIT SLSVPGAHL QCTPRNYPT 
      QSSSLFGDL ATKLSQAYV IWRGAGMSI QGVRTCGDY 
      MFSGHTVAL TMLNFFITE YTPRHIYYL HTLSWMMNM 
      FGIFFILAA HEHYSIDVF VAFYITSRL FLYYHTLSN 
      NQALMQRDS TRTKVWFPL FSYFESSVD GIVPNEYET 
      PSEIVCSLV VFIKSKLSF LKDLLTVIT VRSSDSVNT 
      NHVVDTVRE NKNK
    CDS sequence
    • >TC010470 RA
      atgtcggac cctccagtc atggagtgg agcgtgagt 
      gacacggaa gtgtggtta aggaaggaa ggtttcgac 
      gacaccgtt atcaaaatc ctttgccat gaaaaccag 
      ctcaacggt aaatgtctt ctcgtccta aatgagcac 
      gacttcgcc gccgaaccc ctcagctcg ctcacgctg 
      agggaccgg aagtgtctc tacatagcg tgcaagacc 
      ctccaaagg gacaaccag aatgtcttg atcgatctg 
      ggcatcctc gaaatacca aacgtcaat ctgtacgcc 
      cctcagcag catttcaat aacaagcac gaatgcagc 
      gagtacagc gagtctgag cgaatttct ccacccgtc 
      tctgaagat gggcgcata ccaagactg cccccagaa 
      aagaccaaa gcctttgtt agtttttgt tacgttgtt 
      ggtgttact tggatcacc gcctttgtg atggtcata 
      gttcatgat cgggtcccc gatatgaag aaatatccg 
      cctcttcca gacattttc ctagataat gtcccacac 
      ataccatgg gcctttgat atgtgcgaa attacggga 
      acttttctg ttttccatt tggttagct gtactgctt 
      tttcataga cacagattc atcttaatg cggcgattt 
      ttcgccctc tcggggacg gttttcctg ctccgttgc 
      gtgacaatg ctcataaca tccctcagc gtcccagga 
      gcgcatctc cagtgcacc ccccgaaac taccccaca 
      caaagctcc tctctcttc ggcgacttg gccacaaaa 
      ctgtctcaa gcgtatgtc atatggcgg ggcgccggc 
      atgtcaatc caaggagtg cggacctgt ggcgactac 
      atgttcagt ggccatact gtggcatta accatgttg 
      aattttttc atcactgaa tatacgcca cgccacatt 
      tattacctc catacgctc tcatggatg atgaacatg 
      ttcggaatc ttcttcatc ttagctgct catgaacac 
      tactccatc gatgtcttc gtcgcgttt tatattact 
      agtcggtta tttttgtat taccacact ttgtctaac 
      aaccaagcg ctcatgcaa agagattcc acaaggacg 
      aaggtctgg tttcccctt ttttcgtac tttgagtcg 
      agcgttgat ggcattgta cctaacgag tatgagacc 
      ccaagtgaa attgtctgc agtttggtg gtttttatt 
      aagagtaag ttgagtttt ctgaaggat ttactgact 
      gtcataacg gttaggagt agcgatagt gttaatact 
      aaccatgtg gtagacaca gtaagagaa aacaaaaac 
      aaatag
    mRNA sequence
    • >TC010470 RA
      gatctctgg aatgagtgg aatggaaac aaaaaaatg 
      tgtcaagaa acgccaaac gtcacatgt aggttagtc 
      acaagtttc ttccctgaa aacagcttc tatcactca 
      aagtgagca gttgaaata atgttcagc taacaatca 
      gtgctgcat ccttctcga ataatgtcg gaccctcca 
      gtcatggag tggagcgtg agtgacacg gaagtgtgg 
      ttaaggaag gaaggtttc gacgacacc gttatcaaa 
      atcctttgc catgaaaac cagctcaac ggtaaatgt 
      cttctcgtc ctaaatgag cacgacttc gccgccgaa 
      cccctcagc tcgctcacg ctgagggac cggaagtgt 
      ctctacata gcgtgcaag accctccaa agggacaac 
      cagaatgtc ttgatcgat ctgggcatc ctcgaaata 
      ccaaacgtc aatctgtac gcccctcag cagcatttc 
      aataacaag cacgaatgc agcgagtac agcgagtct 
      gagcgaatt tctccaccc gtctctgaa gatgggcgc 
      ataccaaga ctgccccca gaaaagacc aaagccttt 
      gttagtttt tgttacgtt gttggtgtt acttggatc 
      accgccttt gtgatggtc atagttcat gatcgggtc 
      cccgatatg aagaaatat ccgcctctt ccagacatt 
      ttcctagat aatgtccca cacatacca tgggccttt 
      gatatgtgc gaaattacg ggaactttt ctgttttcc 
      atttggtta gctgtactg ctttttcat agacacaga 
      ttcatctta atgcggcga tttttcgcc ctctcgggg 
      acggttttc ctgctccgt tgcgtgaca atgctcata 
      acatccctc agcgtccca ggagcgcat ctccagtgc 
      accccccga aactacccc acacaaagc tcctctctc 
      ttcggcgac ttggccaca aaactgtct caagcgtat 
      gtcatatgg cggggcgcc ggcatgtca atccaagga 
      gtgcggacc tgtggcgac tacatgttc agtggccat 
      actgtggca ttaaccatg ttgaatttt ttcatcact 
      gaatatacg ccacgccac atttattac ctccatacg 
      ctctcatgg atgatgaac atgttcgga atcttcttc 
      atcttagct gctcatgaa cactactcc atcgatgtc 
      ttcgtcgcg ttttatatt actagtcgg ttatttttg 
      tattaccac actttgtct aacaaccaa gcgctcatg 
      caaagagat tccacaagg acgaaggtc tggtttccc 
      cttttttcg tactttgag tcgagcgtt gatggcatt 
      gtacctaac gagtatgag accccaagt gaaattgtc 
      tgcagtttg gtggttttt attaagagt aagttgagt 
      tttctgaag gatttactg actgtcata acggttagg 
      agtagcgat agtgttaat actaaccat gtggtagac 
      acagtaaga gaaaacaaa aacaaatag tcacgtctt 
      cgtaggaaa ttgttaatt tattaagtt atttattgt 
      gtaaataaa agacttatt tataacggt taatttttt 
      ataacgttt tacaaattg ttacaaatt atgatttac 
      ctatgccaa agttgtgta ttgtctaaa agtattagt 
      tttgtggaa ctttttgca tcagtatga tttattgtt 
      ttgtaataa ttgttaatt atccctaag ccttccatt 
      actattcca ataccgaaa atatgttat ctagtttta 
      acttattga ttcagctac accaaccca cttttgttc 
      ttgataagg atatagttg aacctgtta ttttgaaaa 
      taaatattt ttatatctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY