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Gene information
General information
TC014142 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6586810..6599817
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014142
    iBeetle screen iB_02790

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014142 RA
      MLTELLKYT GMQEKAKLE TKALKAKRP GFTDDRYNE 
      TSYYIENGL RKVYPYYFT FTTFTKGRW VGERILDVF 
      AREFRAHPA EEYERCIQA GTLTVNYDK VPIDYKLKH 
      NDLLANVVH RHEVPVTSQ PITIVHMDE DIVVVNKPA 
      SIPVHPCGR YRHNTVVFI LAKEYNLKN LRTIHRLDR 
      LTSGLLLFG RSPKKARQM EHQIRNRLV QKEYVCRVE 
      GEFPDEIIE CKEPIEVVS YKIGVCKVS PKGKDCNTI 
      FQKLGFNGK TSIVLCKPK TGRMHQIRV HLQYLGYPV 
      VNDPLYNHE VFGPVKGKG GDLGGKTDE ELVRDLIHI 
      HNAENWLGM DGDSELSMF NPRKGDLDD SLVGPLVPK 
      DRVLGDDDA DPVSREPSP CDESCDNNT SISCESLPH 
      PLAVSSLTT SRNVDAKVT VATQTGHEA PDFSFNPDK 
      MTVDKHCYE CKVRYRDPK PQDLVMYLH AWKYRGPGW 
      EFETELPDW ARVDWVDPD ISE
    CDS sequence
    • >TC014142 RA
      atgttgact gaactatta aaatatacg gggatgcaa 
      gaaaaggcg aaactggaa accaaagct ctgaaagcc 
      aaacgaccg ggcttcacg gatgatcgc tacaacgaa 
      acgtcttac tacatcgaa aacgggctg cggaaagta 
      tatccttac tatttcact ttcacaaca ttcactaaa 
      gggaggtgg gtcggcgag cggatcttg gacgtattt 
      gctcgagaa tttcgagcg catcccgcc gaagagtac 
      gaaaggtgc atccaagca ggaactcta actgtaaat 
      tatgacaag gtccctata gactacaag ctaaaacat 
      aatgacctt ctggccaac gtagtccac agacatgaa 
      gtgcccgtc actagccaa cccatcacc attgtacac 
      atggatgag gacattgtg gtggtcaac aaacctgcc 
      tcaatccct gtccatcct tgtggtaga tatcgacac 
      aacaccgta gtttttata cttgccaaa gaatataac 
      ttgaagaat ttaaggaca atacataga ctggatcga 
      ttaacaagt ggtctgtta ctttttggc agatctcca 
      aagaaagct agacaaatg gaacaccaa atacggaac 
      agactggtg cagaaagaa tacgtctgt agagtggaa 
      ggcgagttc ccagacgaa ataatagaa tgtaaagaa 
      cctattgaa gtcgttagc tacaaaata ggcgtctgt 
      aaggtctct cctaaagga aaagactgc aacaccatt 
      tttcaaaaa ttaggtttt aacggaaaa actagcatt 
      gttctgtgt aaacccaag accggacga atgcaccaa 
      atcagggta cacttacag tatttagga tatcccgtc 
      gtgaacgac ccgttgtac aaccacgaa gtcttcggc 
      ccagtgaag ggcaaagga ggagacttg ggggggaaa 
      acggacgaa gaattagta cgtgacctt atccacatt 
      cataatgca gagaattgg ttaggaatg gacggagat 
      agcgaatta tctatgttc aatcccaga aagggagat 
      ctggacgac tccttggtg ggacctctt gtgcctaaa 
      gatcgagtt ttaggagac gacgacgca gacccagtg 
      tcgagagaa ccatcaccg tgtgatgaa tcctgtgac 
      aacaacact tcgatttct tgcgaatct ttaccgcat 
      cctttagcc gtatcgtca ctcaccaca agccgaaac 
      gtcgacgca aaagtcact gtcgcaacg caaacgggc 
      cacgaagcg ccagacttt agctttaat cccgacaaa 
      atgacagtg gacaagcac tgttacgaa tgcaaggtc 
      cggtatcgg gaccccaaa ccacaagat ttagttatg 
      tatttgcac gcttggaaa tacaggggc cccggctgg 
      gaattcgag acggagctc ccggactgg gcgagagta 
      gactgggta gatccagac atatccgag tga
    mRNA sequence
    • >TC014142 RA
      cttgagtga aaatgttga ctgaactat taaaatata 
      cggggatgc aagaaaagg cgaaactgg aaaccaaag 
      ctctgaaag ccaaacgac cgggcttca cggatgatc 
      gctacaacg aaacgtctt actacatcg aaaacgggc 
      tgcggaaag tatatcctt actatttca ctttcacaa 
      cattcacta aagggaggt gggtcggcg agcggatct 
      tggacgtat ttgctcgag aatttcgag cgcatcccg 
      ccgaagagt acgaaaggt gcatccaag caggaactc 
      taactgtaa attatgaca aggtcccta tagactaca 
      agctaaaac ataatgacc ttctggcca acgtagtcc 
      acagacatg aagtgcccg tcactagcc aacccatca 
      ccattgtac acatggatg aggacattg tggtggtca 
      acaaacctg cctcaatcc ctgtccatc cttgtggta 
      gatatcgac acaacaccg tagttttta tacttgcca 
      aagaatata acttgaaga atttaagga caatacata 
      gactggatc gattaacaa gtggtctgt tactttttg 
      gcagatctc caaagaaag ctagacaaa tggaacacc 
      aaatacgga acagactgg tgcagaaag aatacgtct 
      gtagagtgg aaggcgagt tcccagacg aaataatag 
      aatgtaaag aacctattg aagtcgtta gctacaaaa 
      taggcgtct gtaaggtct ctcctaaag gaaaagact 
      gcaacacca tttttcaaa aattaggtt ttaacggaa 
      aaactagca ttgttctgt gtaaaccca agaccggac 
      gaatgcacc aaatcaggg tacacttac agtatttag 
      gatatcccg tcgtgaacg acccgttgt acaaccacg 
      aagtcttcg gcccagtga agggcaaag gaggagact 
      tggggggga aaacggacg aagaattag tacgtgacc 
      ttatccaca ttcataatg cagagaatt ggttaggaa 
      tggacggag atagcgaat tatctatgt tcaatccca 
      gaaagggag atctggacg actccttgg tgggacctc 
      ttgtgccta aagatcgag ttttaggag acgacgacg 
      cagacccag tgtcgagag aaccatcac cgtgtgatg 
      aatcctgtg acaacaaca cttcgattt cttgcgaat 
      ctttaccgc atcctttag ccgtatcgt cactcacca 
      caagccgaa acgtcgacg caaaagtca ctgtcgcaa 
      cgcaaacgg gccacgaag cgccagact ttagcttta 
      atcccgaca aaatgacag tggacaagc actgttacg 
      aatgcaagg tccggtatc gggacccca aaccacaag 
      atttagtta tgtatttgc acgcttgga aatacaggg 
      gccccggct gggaattcg agacggagc tcccggact 
      gggcgagag tagactggg tagatccag acatatccg 
      agtgatgac tactgcgaa acttctttc cctaatata 
      aggcatctc ttatccact ctgtctttc catgctctt 
      agattattg ttgtagggt caagcagcc caagcaaag 
      gcagggtcc aaaaaacaa tcatttcgt tagattgcg 
      ttgaatata ggttaaata aatgaatta gttgaattg 
      aagggtaaa gttgattaa gttttagag agttttagg 
      catgttacg ttgcttaaa ttcaaccac aactttgtg 
      ggtatttca gtgctatct tgagcggtt tttaaggtc 
      ggcgtctag tactcattg tataatgaa aattgcaat 
      caaatattt ttatataag aatatcgtg tctaaagga 
      gtcacttga aactctatt gtagtcgtt acaataagt 
      gccatgatt tgtataaat atctgcgga ttgttgcac 
      cagcagatt ttcatttct tctcatcaa taaaagtac 
      tggaataca tattgtgcc ttgaaagtg tgttgtgaa 
      atcatttgc agattctct atcgaaaat atcccgact 
      ctcatatca gcttcggac ccttgctta gtcaatttt 
      aacttactt attgttatt agcgttatt actttcgaa 
      gcactgaca gggattgtt atgtgacct ttgtagaca 
      ggtaggccc ccaaatatt ttcctttct tattttagc 
      cgctttaga attaattag aattagtta aagaactgc 
      aacaatcta ttcaggtta attatcaca ggacatcgt 
      tttattttt ttttttatt aataaagtt atcaatttt 
      agtgatatt acaataagt tgaaaataa cttaacaga 
      agttttgga gaaggtgaa aataggcct agagaatta 
      ttttcaaat cgatcacaa tttttttta aacaacaaa 
      gtcggcata cataaagaa cactacctc atttttcta 
      gagcggcga tttacgtgt ttaattccg aaaattttt 
      tggtgtcct tttaaatag tttcattcc ttttacatg 
      ggatgttat ttattgaat tccaagtta aagttaaca 
      attaatgaa gtcagtgct ttattatcg gataatttc 
      aaataaaga cactttaca aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY