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Gene information
General information
TC013746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 3837354..3846578
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013746
    iBeetle screen iB_02788
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013746 RA
      MCDDDVCAL VIDNGSGMC KAGFAGDDA PRAVFASVV 
      GRPRYQGVM VGMGQKDSY VGDEAQTKR GVLTVKYPI 
      EHGIVSNWD DMEKIWHHT FYNELRVSP EEHPVLLTE 
      APLNPKINR EKMTQIMFE TFGCPAMYV AIQAVLSLY 
      ASGRTTGIV MDSGDGVSH TVPIYEGYA IPHAILRMD 
      LAGRDLTDY LMKILTERG YSFTTTAER EIIRDLKEK 
      ICFVALDFE RDLALARTM NNFEKSYEL PDGQIITIG 
      NERFRCPEA MFQPTFLGM ETTGIHETT YNSIMRSDM 
      DIRKDLYAN IVLSGGSTM FLGMADRIQ KEIAALAPA 
      SMKIKIIAP PERKYSVWI GGSILASLT TFQQMWISK 
      DEYDECGPV IVHRKCF
    CDS sequence
    • >TC013746 RA
      atgtgcgac gacgacgtt tgcgctttg gtgatcgac 
      aacggctcg ggaatgtgc aaagcgggc ttcgcaggg 
      gacgacgcc ccaagggcc gttttcgcg agcgtggtt 
      ggccggccc cgatatcag ggtgtgatg gtcggcatg 
      ggccagaaa gactcgtac gtgggtgac gaagcacaa 
      accaaacgg ggcgttttg acggtcaag tacccaatt 
      gagcatgga attgtgagt aattgggac gatatggag 
      aaaatctgg catcacact ttttacaac gagttgagg 
      gtctctcca gaggagcat cccgtgctc ctgaccgag 
      gctcccctt aacccgaaa ataaaccgg gagaagatg 
      actcaaatc atgtttgag acgttcgga tgcccggcc 
      atgtatgtg gcgattcag gccgttttg tctctgtat 
      gccagtggc aggacgacg gggattgtg atggattct 
      ggcgatggg gtttcccat acggtgccc atttatgaa 
      ggttatgcc atcccgcac gcaatcttg cggatggac 
      ctcgccgga cgcgaccta accgattac ttgatgaaa 
      atcctaacc gaacgcggt tacagtttc acgacgacc 
      gcagagcgc gaaataatc cgcgatttg aaggagaaa 
      atctgcttc gtagctttg gattttgaa cgagacctc 
      gcactcgcg agaactatg aacaatttt gagaaaagt 
      tacgaattg ccagatgga caaatcata acaatcggg 
      aatgaacgt ttccggtgc ccggaagct atgtttcaa 
      ccgactttt ctgggaatg gaaacaaca ggaattcac 
      gaaaccact tataattcg attatgagg tctgacatg 
      gatataagg aaggatctt tatgctaat attgtgctc 
      tctggagga agtacaatg tttttgggg atggccgat 
      aggattcag aaggagata gctgcccta gcgccggct 
      tcaatgaaa attaaaatt atcgcaccg cctgagaga 
      aaatattcg gtttggatt gggggaagt attctggcg 
      agtctgacg acgtttcaa caaatgtgg atctcgaag 
      gatgaatat gatgagtgc ggacctgtt atcgttcat 
      cgaaagtgc ttctaa
    mRNA sequence
    • >TC013746 RA
      tgcagtgtg tgtttaccg taaaaaagc aatctgaag 
      taattaagt gaattgtac catttcata acggtccga 
      aacacagtg aatgcttga aatgtttaa aaattaatc 
      cattttatt gcaattaaa agtgatcaa acaaccata 
      ccgaaatca ccacttcag tcgagcggt catttttgt 
      gtttggaga tatctttga gttataatt ttgaaaaat 
      gtgcgacga cgacgtttg cgctttggt gatcgacaa 
      cggctcggg aatgtgcaa agcgggctt cgcagggga 
      cgacgcccc aagggccgt tttcgcgag cgtggttgg 
      ccggccccg atatcaggg tgtgatggt cggcatggg 
      ccagaaaga ctcgtacgt gggtgacga agcacaaac 
      caaacgggg cgttttgac ggtcaagta cccaattga 
      gcatggaat tgtgagtaa ttgggacga tatggagaa 
      aatctggca tcacacttt ttacaacga gttgagggt 
      ctctccaga ggagcatcc cgtgctcct gaccgaggc 
      tccccttaa cccgaaaat aaaccggga gaagatgac 
      tcaaatcat gtttgagac gttcggatg cccggccat 
      gtatgtggc gattcaggc cgttttgtc tctgtatgc 
      cagtggcag gacgacggg gattgtgat ggattctgg 
      cgatggggt ttcccatac ggtgcccat ttatgaagg 
      ttatgccat cccgcacgc aatcttgcg gatggacct 
      cgccggacg cgacctaac cgattactt gatgaaaat 
      cctaaccga acgcggtta cagtttcac gacgaccgc 
      agagcgcga aataatccg cgatttgaa ggagaaaat 
      ctgcttcgt agctttgga ttttgaacg agacctcgc 
      actcgcgag aactatgaa caattttga gaaaagtta 
      cgaattgcc agatggaca aatcataac aatcgggaa 
      tgaacgttt ccggtgccc ggaagctat gtttcaacc 
      gacttttct gggaatgga aacaacagg aattcacga 
      aaccactta taattcgat tatgaggtc tgacatgga 
      tataaggaa ggatcttta tgctaatat tgtgctctc 
      tggaggaag tacaatgtt tttggggat ggccgatag 
      gattcagaa ggagatagc tgccctagc gccggcttc 
      aatgaaaat taaaattat cgcaccgcc tgagagaaa 
      atattcggt ttggattgg gggaagtat tctggcgag 
      tctgacgac gtttcaaca aatgtggat ctcgaagga 
      tgaatatga tgagtgcgg acctgttat cgttcatcg 
      aaagtgctt ctaatgtat aatgaaaaa aaattattt 
      atcaaaact agaatacaa tacttttgt taataaacg 
      acaaatcat tttgtaaaa aatggtgct gtgtaaaca 
      tcaataaat atcgtaaaa tgtt