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Gene information
General information
TC033036 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3474878..3490339
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033036
    iBeetle screen iB_02787

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033036 RA
      MSLLITANL PHARFMVWC RPSNGRKRL TRKNDRKFE 
      VYFGGGGAK RDEWRVSAC VIFKRRDAS LRNGVITPE 
      QCPSVVSLL AESYNPHVR YGAAMALGI ACAGTGLRE 
      AIALLEPMV MFDPVNFVR QGALIASAM ILIQQTEQT 
      CPKVSFFRQ TYAQVISNK HEDVMAKFG AILAQGIID 
      AGGRNVTLS LQSRTGHMN MLAVVGTLV FTQYWYWFP 
      LSHCLALAF TPTCLIALN EQLKMPKLE LKSNAKPSL 
      YAYPAPLEE KKREEREKV TTAVLSIAA RQRRRDHDR 
      KHRDEKMEV DEESKDEKK SEEKKTEDD KKKTDDKKT 
      EKKDEKEEK KKEPEPNFE ILNNPARVM RQQLKVVTI 
      NDNSQYVPL KDVSIGGIV MVRNLKPGE EELVENVAA 
      FGPKGDDEK EPEPPEPFE WLED
    CDS sequence
    • >TC033036 RA
      atgtcgcta ttaattacc gcaaacctg cctcacgcc 
      cggttcatg gtgtggtgt aggccttcg aacgggagg 
      aagagattg acgaggaaa aatgaccgc aaattcgag 
      gtttatttc ggtggcggt ggcgcgaaa cgggacgag 
      tggcgcgtg tccgcgtgc gtcattttt aagcgtcgc 
      gacgcgtct ctccggaac ggcgtcata accccggag 
      caatgcccc agtgttgtt tccctcctc gctgaaagc 
      tacaacccg cacgtgcgt tacggcgct gcgatggct 
      ttgggcatc gcttgtgcc ggaactggg ctccgggaa 
      gctatagct ttactggag ccgatggtt atgtttgat 
      ccggtgaat ttcgtccgt caaggagct ctgattgca 
      tcagctatg attctgatc caacaaacg gaacagact 
      tgtcccaaa gtcagtttc ttccgccag acttacgct 
      caagtcatt tccaacaaa cacgaggat gtgatggcg 
      aaattcggg gcgattttg gcgcaagga attatcgat 
      gcaggagga agaaacgtg actttgtcg ctgcaatcc 
      aggaccggg catatgaac atgttggcc gttgtgggg 
      actttggtc tttacgcag tattggtat tggttccct 
      ttgtcgcat tgtctggca cttgcgttc acgccgact 
      tgtcttatc gcactcaac gaacaattg aagatgccc 
      aaactggaa ttaaaatct aacgcgaaa ccgagcttg 
      tacgcatat cctgcacct ctggaagag aaaaagcgg 
      gaagaacga gagaaagtc actactgct gttttgagt 
      attgcggca aggcagcgt cgacgagac cacgacagg 
      aaacatcgc gatgaaaaa atggaagtc gatgaagag 
      agtaaagac gagaagaag tcagaagag aagaaaact 
      gaagacgac aagaagaaa accgacgat aagaaaacc 
      gagaaaaag gatgaaaaa gaggaaaag aagaaagag 
      cccgaaccc aattttgaa attcttaat aatccggca 
      cgagttatg cgccaacaa ctcaaagtt gttacaatt 
      aatgataat agtcagtac gttcctttg aaggatgtt 
      tccattggc gggattgtt atggttcgt aatttgaag 
      ccaggagaa gaagagttg gtcgaaaat gtggctgct 
      tttgggccg aaaggtgac gatgagaag gagccagag 
      ccaccagaa ccattcgaa tggcttgaa gattag
    mRNA sequence
    • >TC033036 RA
      agcccgcca ccgcccgcc gccgccacc acctcctat 
      cgaacgcgt atccctccg acaatggtt gcgggcttg 
      atttatgtc gctattaat taccgcaaa cctgcctca 
      cgcccggtt catggtgtg gtgtaggcc ttcgaacgg 
      gaggaagag attgacgag gaaaaatga ccgcaaatt 
      cgaggttta tttcggtgg cggtggcgc gaaacggga 
      cgagtggcg cgtgtccgc gtgcgtcat ttttaagcg 
      tcgcgacgc gtctctccg gaacggcgt cataacccc 
      ggagcaatg ccccagtgt tgtttccct cctcgctga 
      aagctacaa cccgcacgt gcgttacgg cgctgcgat 
      ggctttggg catcgcttg tgccggaac tgggctccg 
      ggaagctat agctttact ggagccgat ggttatgtt 
      tgatccggt gaatttcgt ccgtcaagg agctctgat 
      tgcatcagc tatgattct gatccaaca aacggaaca 
      gacttgtcc caaagtcag tttcttccg ccagactta 
      cgctcaagt catttccaa caaacacga ggatgtgat 
      ggcgaaatt cggggcgat tttggcgca aggaattat 
      cgatgcagg aggaagaaa cgtgacttt gtcgctgca 
      atccaggac cgggcatat gaacatgtt ggccgttgt 
      ggggacttt ggtctttac gcagtattg gtattggtt 
      ccctttgtc gcattgtct ggcacttgc gttcacgcc 
      gacttgtct tatcgcact caacgaaca attgaagat 
      gcccaaact ggaattaaa atctaacgc gaaaccgag 
      cttgtacgc atatcctgc acctctgga agagaaaaa 
      gcgggaaga acgagagaa agtcactac tgctgtttt 
      gagtattgc ggcaaggca gcgtcgacg agaccacga 
      caggaaaca tcgcgatga aaaaatgga agtcgatga 
      agagagtaa agacgagaa gaagtcaga agagaagaa 
      aactgaaga cgacaagaa gaaaaccga cgataagaa 
      aaccgagaa aaaggatga aaaagagga aaagaagaa 
      agagcccga acccaattt tgaaattct taataatcc 
      ggcacgagt tatgcgcca acaactcaa agttgttac 
      aattaatga taatagtca gtacgttcc tttgaagga 
      tgtttccat tggcgggat tgttatggt tcgtaattt 
      gaagccagg agaagaaga gttggtcga aaatgtggc 
      tgcttttgg gccgaaagg tgacgatga gaaggagcc 
      agagccacc agaaccatt cgaatggct tgaagatta 
      ggtttagtt gcgtgcttg tgtctctat tattcatat 
      aatattaaa attattgta acttaatgt ttagaaaaa 
      taaaataaa aaatgaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY