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Gene information
General information
TC013839 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3279898..3281333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013839
    iBeetle screen iB_02785

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013839 RA
      MFTRKSTLV KKTGPDRVG RYEFLKQLI 
      VEFGTTKSL EAKKQVLAN LANFSYDPV 
      NFEFLKQLH AIDLFLAQL SEDNEDLLH 
      FGLSALCNL SPDEECKDY IIKLNGIKL 
      ISDRLFHKN EEIALNAIT TLYYLIYPS 
      NKHLLTREI LDKVQFFES HQNPRYKNL 
      GTIFMEESK ND
    CDS sequence
    • >TC013839 RA
      atgtttact agaaaatcc actttggtg aaaaagaca 
      ggccctgac cgtgtgggc cgttacgaa ttcctcaaa 
      caattaatc gtggaattt gggacaaca aaatcgctc 
      gaggccaag aagcaagtt ttggccaat ttggcgaat 
      ttttcgtac gaccccgtc aatttcgag tttttgaag 
      caactccat gccattgac ttgttcctt gcacaactt 
      tccgaagac aacgaggat ttgttacat tttggcctc 
      tcagcattg tgtaactta agtccagac gaggaatgc 
      aaggattac atcattaaa ctcaacgga attaaacta 
      atttcggac agactgttc cacaaaaac gaggaaatc 
      gcactgaat gccatcaca accttgtat tatttaatt 
      tacccctca aacaagcat ttactaact cgggaaatc 
      ttggataaa gttcagttt ttcgaatcc caccagaac 
      ccccgctac aaaaacctg gggacaatt tttatggaa 
      gaatcaaaa aatgattag
    mRNA sequence
    • >TC013839 RA
      cattttttt attcaacag taaaaaaca agcaaaata 
      ttattcttc aggtgtgat tacaatcat tttgtttta 
      catggtaag gggtcacag ctcaagtgt tttcgattt 
      aactaaaaa tatgtggtt cgagtgggt ttaaaagtt 
      tgagtggta ccaaacaat tgaagtaaa aataccagt 
      tattatttt tttttctaa ctggaaaat gcccaccaa 
      atagtttaa ttttgcttt atttcttgt aattgttat 
      taaagaagt actatgtag aaagttagg caaccaaca 
      aaatccgag attagttcc ttgtcattg tcaaggttg 
      gcacaaggt cggttacgt aacataacc ttatttttc 
      cataaaatg ctctcggaa tatgtttac tagaaaatc 
      cactttggt gaaaaagac aggccctga ccgtgtggg 
      ccgttacga attcctcaa acaattaat cgtggaatt 
      tgggacaac aaaatcgct cgaggccaa gaagcaagt 
      tttggccaa tttggcgaa tttttcgta cgaccccgt 
      caatttcga gtttttgaa gcaactcca tgccattga 
      cttgttcct tgcacaact ttccgaaga caacgagga 
      tttgttaca ttttggcct ctcagcatt gtgtaactt 
      aagtccaga cgaggaatg caaggatta catcattaa 
      actcaacgg aattaaact aatttcgga cagactgtt 
      ccacaaaaa cgaggaaat cgcactgaa tgccatcac 
      aaccttgta ttatttaat ttacccctc aaacaagca 
      tttactaac tcgggaaat cttggataa agttcagtt 
      tttcgaatc ccaccagaa cccccgcta caaaaacct 
      ggggacaat ttttatgga agaatcaaa aaatgatta 
      gggttcgcc agttttcca ccaaagcta cattcctca 
      aacgggtca ctaaaaaag acttggagc agtttacag 
      aattgagtg gcgacacga acccgattc attcgacaa 
      aagggccac agagggcga tagtacacg gagcattac 
      tcaattgtt acgtttcta aagtcatag ggacgcagt 
      taccagggc cgggctcag tcgtggtgg cccaaactt 
      tgaatttcc cgcataaat gctacgagg gcgatgagg 
      tgaaaatca ctgtggaaa tggcggtag agcggaaaa 
      tcaccacag ttaaattca cctgtgaag tacccgaac 
      gggagcaag tggtacttt acggcgatg ctaaattaa 
      ttccgccga aaaataaat aatttattt aaaattttt 
      gtttcattt tgaaaaaaa aaacaaata aagtgggtc 
      atgttgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY