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Gene information
General information
TC010933 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1468679..1472342
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010933
    iBeetle screen iB_02784

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010933 RA
      MKFLAFFLL CVPHVLSLP KGSRIVGGN QARGGQFPF 
      VAAIYVTRS GGTYFCGGV LLSNQWVLT AGQCVDGAT 
      SFRVQLGSN ILEGTDLNR VTLATATYT LHPRYNSET 
      LENDLGLIK LRLPIEFNQ YISVITLPS SFTPDYVSV 
      TAVGWGQVD DFSTGLSNN LVSVTLTTI SHDECQLTY 
      GPQITHDML CAAGNFNEG ICNGDMGGA LIQTIGGTA 
      VHVGIASFR SHNGCETTD PSGFTRTIA YVDWIRNFV 
      ALFSLFTLF SSPVYSNIA GRIIGGSDA KAGEFPFTA 
      AIYIHTSSG QYFCAGALL SREWILTGA QCVDGALQF 
      TIVLGSNNL QDSNDPNRV KVATEEYYL YPKYDAFTL 
      QHDIALIKL RLPIEYSDY IQPILLPTA ELVDYSVVV 
      ALGWGQTSD EDAGNVSDL HRVSITVLS NQECRNTYG 
      TQIIEEMVC AEGNYNQGT CHGDSGGPL IQYVYGQSA 
      THVGVSSFF SGNGCESTD PSGFTRTFP YVEWIRNVT 
      QI
    CDS sequence
    • >TC010933 RA
      atgaaattc ctcgcattc tttttgctg tgtgtacca 
      cacgttttg agtttgcct aaagggtct cgaattgtt 
      ggaggaaac caggccaga ggtggtcag tttcccttt 
      gtggcggca atttatgta acaaggtct ggaggtact 
      tacttctgt ggaggtgtt ttgcttagt aatcagtgg 
      gttctcacg gctggacaa tgcgtagat ggagccact 
      tcgtttcga gttcaactt ggctcaaat attctggaa 
      ggtacagat cttaatcgt gttacttta gccaccgct 
      acttacacc cttcatccc aggtataat tccgagact 
      ttggaaaat gatcttggt ttgattaaa ttgcgactt 
      ccgattgag ttcaatcaa tatatcagt gttatcaca 
      ctaccaagt agtttcacc cctgattat gtttccgtc 
      actgcggtt ggatgggga caagtcgat gattttagt 
      accgggctt tcgaacaat cttgtttcc gtcacactt 
      actacaata tctcatgat gagtgtcag ttaacttac 
      ggtccacaa attacgcat gatatgctc tgtgctgct 
      ggaaacttc aatgagggt atttgtaat ggtgatatg 
      ggtggagct ttaattcaa acaattggt ggaacagca 
      gttcatgtt ggaattgca agtttcaga agtcacaac 
      ggatgtgaa accactgat ccttcaggt tttactagg 
      acaattgct tacgtagac tggataaga aattttgtt 
      gcacttttt tccctattc acacttttt tcatcacca 
      gtttatagt aacatagct ggacgcatt attggtggc 
      agtgatgct aaagctgga gaattccca ttcactgca 
      gcaatttac attcatacc agttcaggc cagtatttt 
      tgtgctgga gctctgctt agccgcgag tggattttg 
      accggtgct caatgtgtt gatggtgcc cttcagttt 
      actatagtt cttggttct aacaatttg caagatagt 
      aatgacccc aacagagtt aaagtagct actgaagag 
      tactatctg tatccaaag tatgatgcg ttcactctt 
      cagcatgat attgctctt attaagttg cgtctgcct 
      atagaatat agcgattac attcaacca atcctttta 
      ccaactgca gaactggtc gattattcg gtagttgta 
      gcgcttggg tggggacaa accagtgat gaggatgcc 
      ggcaatgtt tctgatctt cacagagtt agcatcacg 
      gttttaagt aaccaggaa tgtcgaaac acttacggg 
      actcaaatt atcgaagaa atggtctgt gctgaagga 
      aattacaac caaggaact tgccatggt gatagtggt 
      ggtcctttg attcaatat gtttacgga caatctgcc 
      acacatgtc ggtgtgtct agctttttc agtggaaat 
      gggtgtgaa tcaaccgat ccgtctggt tttacaagg 
      acttttccc tatgttgaa tggataaga aatgtaaca 
      caaatctaa
    mRNA sequence
    • >TC010933 RA
      tttttttaa caaatatct agacaacca tattattga 
      atttctgta ttcacaata aaagaatgt taagccgcc 
      aaaccacta cttaatatt aaatcgttt tgacaatag 
      aagataaat aatccagtt aagcatatc ctagattac 
      ggtaaggat aaattacaa ttcacaaca tcaaagatc 
      atttcatca tgaaattcc tcgcattct ttttgctgt 
      gtgtaccac acgttttga gtttgccta aagggtctc 
      gaattgttg gaggaaacc aggccagag gtggtcagt 
      ttccctttg tggcggcaa tttatgtaa caaggtctg 
      gaggtactt acttctgtg gaggtgttt tgcttagta 
      atcagtggg ttctcacgg ctggacaat gcgtagatg 
      gagccactt cgtttcgag ttcaacttg gctcaaata 
      ttctggaag gtacagatc ttaatcgtg ttactttag 
      ccaccgcta cttacaccc ttcatccca ggtataatt 
      ccgagactt tggaaaatg atcttggtt tgattaaat 
      tgcgacttc cgattgagt tcaatcaat atatcagtg 
      ttatcacac taccaagta gtttcaccc ctgattatg 
      tttccgtca ctgcggttg gatggggac aagtcgatg 
      attttagta ccgggcttt cgaacaatc ttgtttccg 
      tcacactta ctacaatat ctcatgatg agtgtcagt 
      taacttacg gtccacaaa ttacgcatg atatgctct 
      gtgctgctg gaaacttca atgagggta tttgtaatg 
      gtgatatgg gtggagctt taattcaaa caattggtg 
      gaacagcag ttcatgttg gaattgcaa gtttcagaa 
      gtcacaacg gatgtgaaa ccactgatc cttcaggtt 
      ttactagga caattgctt acgtagact ggataagaa 
      attttgttg cactttttt ccctattca cactttttt 
      catcaccag tttatagta acatagctg gacgcatta 
      ttggtggca gtgatgcta aagctggag aattcccat 
      tcactgcag caatttaca ttcatacca gttcaggcc 
      agtattttt gtgctggag ctctgctta gccgcgagt 
      ggattttga ccggtgctc aatgtgttg atggtgccc 
      ttcagttta ctatagttc ttggttcta acaatttgc 
      aagatagta atgacccca acagagtta aagtagcta 
      ctgaagagt actatctgt atccaaagt atgatgcgt 
      tcactcttc agcatgata ttgctctta ttaagttgc 
      gtctgccta tagaatata gcgattaca ttcaaccaa 
      tccttttac caactgcag aactggtcg attattcgg 
      tagttgtag cgcttgggt ggggacaaa ccagtgatg 
      aggatgccg gcaatgttt ctgatcttc acagagtta 
      gcatcacgg ttttaagta accaggaat gtcgaaaca 
      cttacggga ctcaaatta tcgaagaaa tggtctgtg 
      ctgaaggaa attacaacc aaggaactt gccatggtg 
      atagtggtg gtcctttga ttcaatatg tttacggac 
      aatctgcca cacatgtcg gtgtgtcta gctttttca 
      gtggaaatg ggtgtgaat caaccgatc cgtctggtt 
      ttacaagga cttttccct atgttgaat ggataagaa 
      atgtaacac aaatctaag aaataaatt tttggaaat 
      tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY