Status
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Gene information
General information
TC031117
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007013
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11839696..11907873
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031117
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02781

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031117 RA
      MILLYFVFS CFYNFVFGV NYFGPQPRI VTQDGHLIF 
      YAAVHKNIS LQTQGRGKI HVNGIDLSQ AAKLTNSAF 
      DAMKKYQTN FENTFEKIS KFEQLLDDG LMQKITTLN 
      GRTNGTTQD GVNSASLVR SVRRLNRRV NALQKSVKM 
      LADLLEDDD CQSDPCKNG GTCEDLFNT FLCHCPPEW 
      EGPVCDKDV NECARFAGT DLGCQNGAT CVNRPGSYE 
      CLCSRGWVG LHCRNRNVD CRQGSNFEL CGHGTCITQ 
      YNDVGYKCI CDEGWTTDG KTPACNTDV NECTGTKPH 
      CSVNPPVQC INLPGTYRC GSCPAGYTG DGYYCTDID 
      ECSTSNGGC STNPMVQCI NTLGSRFCG SCPSGYVGD 
      GITCSYQGV CNVNNGGCH PLATCRNNP RIGSTFVEC 
      ICPSDYTGN GIGRNGCVR SLPSAPGPC TPNPCRRGF 
      CRVTNGTDY TCHCNRRYA GKNCEKFRD LCTSNPCQN 
      GATCTSFLG ISYQCLCPS AYTGLRCET EKQSCGGRL 
      VGETGTLVF PSGSSQTYS SAMSCAWIL ETNVTKVLN 
      ITFKRFDVE GSRGCRYDW LQIHDGKNT LARSLGRFC 
      GHQLPNGGN IVSTHNTVY LWFRSDHSV ASEGFELNW 
      TSIVPFCGD QLKSVTSHG IIQSPGSPG NYPNNRDCY 
      WPLQAQPGK RLLFHFFTL MIGNNSACD HDYLEFISG 
      TEPTDPVFA KFCNTTHPS PLFSPSGEV LVHFHSDMD 
      KNYPGFQIT YSVVEGMPG CGGVYTRSQ DEIRSPMYN 
      GNYPNDIQC EYKIQLTTK SRIKLTFLS FDVEDSENC 
      QFDYVAIYA GTTVDAPLI GKYCGQTVP SPYITEKSE 
      ILIVFKTDW ATAGEGFAL RYETVCGGT FTTPSGVIQ 
      SPGYPKNYD HNLECIFEI VQPLGNIIK LNIVDLDLE 
      SNTYPDCVY DFLEVRDGH NSNSTLIGT YCANKVPLI 
      VSSYNYLWI KFQSDTNNN GKGFRANYT TTNIGCGGI 
      MKEKSGTFS TPKYPQVYP SGTICKWII SAPPGNVIQ 
      VTWMNFQIE GSFDCTYDA VEIFDNNTE TGEGTSIGK 
      FCGQHVPPA MLSTSNLIT VIFHSDQTN NMNGFVASY 
      STLLESTVC RGNYYSNAG VIKSPGYPN NYPLNIECT 
      WTIQVKPGH QILLNVTDF QLEHYTTCR YDWLEIRNG 
      GSSMSPLLG RFCNTDIPK MIPSHANKL FIRFKSDMS 
      KSEKGFKIT WEATATGCG GVLNSPTGS IISPNYPEP 
      YNLNAECIW KISISAGSL IQLVFSDLD LEEHSECAL 
      DYVEILDGP SLNSKSLGR FCLHHPSFL RSSGNTLTV 
      IFRSDLSRN GRGFHLQYI TMCNNTLKG FRGVIESPN 
      FPNNYPNSV NCNWEIKVA DKNKINISF SHFDLEKVL 
      VQNVPSNNS DSDKCLFDY VEVLYVEPV EEYEEEGPF 
      QKYGIYCGD KIPPLITLN SDHAKIRFL SDNLLFGNG 
      FRLEWQLEG CGDILTHPN GTIFSPNYP RSYPPSIEC 
      NWKIQVDFG SNVEITFHK IEIEKTYIC HLDYIKLYN 
      GEDETYPEI ATLCHQNKP ITLRSSGNF LFVKFKADS 
      SVQGMGFYA NYTSTPTKC GGKYTADSA FIMSPNYPE 
      NYNKNSTCG YEIQVGEGF RIELKFQDF DLYAPNRLN 
      CTSNNISYV KIYDGPTID FPLLAKICN KNAPNKTIL 
      TTTNQMYIE MVSQTDMAS KGFLAKYEK ACGARIVTS 
      GTGHIRIFS HDLDYDESN CTWTIIAKD LTKHITLTV 
      LYLDSLNGY CDENIKILK VYNGESSEA PLLGEYCQK 
      IPPTLTSDG SAMHVVVDS NTVLYATYS VFDSVCGGT 
      LTSVAGYFA SPGYPKKYP VDTECEWTI DVTEGNHVS 
      LSFSYFGLV ESDHCNTDY LEIRQNNGT GKLLGTFCG 
      SDLPTNITN EGNLWIFLK TSRSDGQVK GFYAEYVLN 
      HENELSGPR GRIVSPLYY QNYPFYGDF SWTITVAFG 
      KRILLTFKD ILLENGDSM CFSSIYIYD GSTDEAPSL 
      GEFCGSTLP DAIKTSSNI VLIKFSGYS TSRSLTKFI 
      IEWLEIESV VNTNKPSPK PNGCGTKEP IALNELKNY 
      TTITSPGFP NGYAPNLQC EWILTTIPM NSLRIYFRE 
      MDLQPTYGG CYADYVEIF EKRLDKDWT SVLKTCLSN 
      ITSNPGIYT TNMMKVVFK SDRYGNKTG FSALVRESC 
      GGTLTAPTD YIIYNNNSR SSYLRTYNS VCQWNITVR 
      SGRTIELEF LYFNIHHDT NKGCDNYLI IRNGKFPDS 
      PILGTGKYC GDQKPPTLK TTGNNAYIK FGGITSLSN 
      FIIRYREIS YACDSEIKL TKFANSTEI HSPNYPNIP 
      PPHVECNWR IMAPPGETL RIDFIDRFD LKVAKNCTH 
      EYVEVRDGG TKYSKIIGK YCEKPNSQF STDNMMFVR 
      FFTELNDPS NGFKARIVL ANCGGSIHS TTGQIQSPF 
      FNIPSKYPV GVNCTWHLI SPQNHNMDI TFEKIDLEE 
      ALNCTGVDH VKIYERNEF DNKEYVLGN FCGKKLPAM 
      VQSSGSEAI VHFETGKIK KELGGFSLK FNASAEVCG 
      GSIEASEGT IKSPGYPNP HKIYRRCSW NIKVPKGRR 
      VTLKIEDFG LDNRFKGSW INGLVIYHD EKFKGYIRK 
      ISASNEIVE SSENHMLVF YWSPATMGR GFKAKFTSN 
      NPSVCDGDF NQDSGTITQ PNIDYTYSC TYVRDKPSN 
      AGTLALTLT TTTNTTIPS NVKNCLTYI AAFKIETVN 
      GKKELFSTN CVPGTSVVR IPHSKIQLS VVQYSSRNQ 
      VNFTLSYNT YQCGGIITR QTGEITSPG YPNKNSKSM 
      ECSWLLQLP KEQTISLTF DSLNLGSNC EKSYIDIYN 
      GDSPKSPKI DRYCQSNKP GTISSQTNN LWIDYKFDI 
      DSDSKGFSL KYEPKIQGC GGIFHDRES IIQTPSFPN 
      DYPNNAECL WELRSVEGF HIGLIFTTR FQIEETDNC 
      ENDFLEIWD WRDDDWVSL GRKCGREFP KTVNSTSNR 
      MKILFRSNE RITGKGFRA QWQWNCGGT FTADRKERF 
      IVSPGFPQE YQKNIDCQY NITSKQSHG RFINIKFYN 
      FDLEGSGSN CRYDNLTLS RFWMRKVNY YHMPATQVF 
      CGQKLPPPI RLGDTLYIR FQTDPWVQK TGFKFSYQL 
      DDCGGQITS PTVISSPLT KIDRSMFPL YTGYMSCIW 
      NITAPPKKS IVISFQKFR FEYRIRCHF DHVQIIEGP 
      VNTIAKRIA NLCGDLNQN LPTIKSENN TVLVVMNVD 
      RSQNHEGFE AEVYFVDGP EAGCGGKIV LNKTMTLEA 
      PNLADMDCL WTIQSQIDT TVKIHFDEL NLPSRCGLT 
      NHTITNCSC AALEVRDGH PGVGDLIER FCGITDTHY 
      SREITTSGN VMGLRLRTR GSNSKAFKV TLTPIASIC 
      GPVYLNVTQ DVKTLLSPN YPNKYPSDL RCSWILKGT 
      SRFVVRFID FDLNKERNQ HVVNNVCGE DKLEIVDNM 
      VNLLTTSDM GPSFTVSGD RKLTTDFLR YGYLTGTHQ 
      FCGRFENPF DFYSQFSQL IIKFKSGNL DKDRGKGFK 
      IEYHTTGCN RNFTSLQGR IVQNDRTQS KSCIQIITV 
      PKNYTISLY FQELTIFHS EGCQNAGIE IRDENSQGT 
      LFQKVCGYY VPNPVFSTK NQLWIRSWN TNSFPISYD 
      LVYTASDQG RGCGGIIYN YKGTFSSPL HPMAYKNST 
      PCVWTLRTA IGRRIAIKF TVFDIQGDC AENYVKVTT 
      YEKNTSNDH KYCKNDDPA VLYSNSRML VTYQSSTKN 
      DGSGWIAKF EAAISDEVM IVLSPPLQI AALCYMFTV 
      TILVFVLGC YIYFLQSNR KRCTCSLAE KDVEMRKAH 
      RKSENNVYE ADRSTKAEH SSSLHQVRM SEYVSITSD 
      SQKRSSPSP PTNDRTVDE TDDSAYFLN AYETIVDSL 
      YISLCKVPK SKQDLYARF LNDLAKKIK MKDSAVYDK 
      LQMCGLLNV NHYEVINEN EPGSEYDSD DCTYVKYVP 
      SESVKQSLM RAQALHRTQ CPKHVYGPN ERLRNCLKE 
      QKDAKNMSR SEKPPIPPK KVRLRKNSE EDVI
    CDS sequence
    • >TC031117 RA
      atgattttg ttgtatttc gttttttct tgtttttac 
      aattttgtg tttggagtt aattatttt ggaccacaa 
      cctagaata gtgactcag gatgggcac ttaatattt 
      tacgccgca gttcacaaa aatattagt ttgcaaacc 
      caaggacga ggaaaaata catgttaat ggaattgac 
      ttatcacag gcagctaaa ttaaccaat agcgcattc 
      gatgccatg aaaaaatac caaacaaat ttcgaaaac 
      actttcgaa aaaatctcc aaatttgag cagcttctg 
      gacgatggt ttgatgcaa aaaatcacg acattgaat 
      gggagaaca aacggaaca acacaagat ggagtaaac 
      agtgcttct ttggtgaga agcgttcga agactaaac 
      cgaagagtc aatgctttg caaaaatct gtaaaaatg 
      ttggctgac cttttagaa gatgatgat tgtcagagt 
      gatccgtgc aaaaacggt ggaacgtgt gaagattta 
      ttcaatact tttctgtgc cattgtcca cctgagtgg 
      gagggccct gtatgtgac aaagatgtg aacgaatgt 
      gccagattt gcaggaacg gatttaggg tgtcaaaat 
      ggggccact tgtgttaac aggcccggc tcatacgag 
      tgtttgtgt tcgcgtggt tgggttggc cttcactgc 
      agaaaccga aatgttgat tgtcgccaa ggttcgaat 
      ttcgagctt tgtggacac ggcacgtgt atcacccaa 
      tacaacgat gttggatat aaatgcatt tgtgacgag 
      ggatggaca acagatggc aaaactcca gcttgtaat 
      acagatgtt aacgaatgc acaggaact aaaccacac 
      tgttcagtt aatccacct gtgcagtgt attaactta 
      cccggaact taccgctgt ggtagctgt ccagctggg 
      tacactggt gatggatac tattgcaca gacattgac 
      gagtgcagt acatcaaac ggtggatgt agcactaat 
      cccatggtg cagtgtatt aatacactt ggatcaaga 
      ttttgcggt agttgtcct agtggatat gtaggagac 
      ggaatcaca tgttcatat caaggtgtt tgtaatgta 
      aataatggc ggttgtcat cctttggcc acttgtaga 
      aataatcca agaattggt tccactttc gttgaatgt 
      atttgtccc agtgactac acaggtaac gggatagga 
      cgcaatggt tgtgtgcgt tcattgcct tcagcccca 
      ggtccttgc accccaaat ccttgcaga cgtggcttt 
      tgcagagtt accaacgga accgattac acgtgccat 
      tgcaacagg cgatacgcc gggaaaaat tgtgaaaag 
      tttcgcgat ttgtgtact tctaaccct tgccaaaac 
      ggcgcaact tgcacttcg ttcttgggc atttcttac 
      cagtgtttg tgtccatca gcttataca ggattgcgt 
      tgcgaaacg gaaaaacaa tcttgtggt ggtcgtctt 
      gtgggagaa accggaact ttggttttt ccttctggt 
      tcgtcacaa acatatagc agtgcgatg agttgtgcc 
      tggattttg gaaactaat gttactaaa gtgttaaat 
      atcactttt aaacgtttc gatgttgaa ggatcacgc 
      gggtgtcgt tatgattgg ctacagata catgatggg 
      aaaaataca ttggcacgg tctttaggt cgtttttgt 
      gggcatcag ttaccgaat ggtggaaat attgtatcg 
      actcataat actgtttat ttgtggttt agatcggat 
      cattcggtt gcatcagaa ggatttgag ttgaactgg 
      acatcgatt gtaccattt tgtggagat caactgaaa 
      agtgtaaca tcgcatgga ataatccaa tcacctgga 
      tcacctgga aattaccca aataatcgt gactgctat 
      tggccactt caagcccaa ccaggaaaa cgcctcctc 
      ttccacttt ttcactcta atgattgga aacaactca 
      gcctgtgat cacgattat ttagaattt atttcagga 
      actgagcct acagatcca gttttcgcc aaattttgt 
      aatactaca cacccctct ccacttttt tctccatca 
      ggcgaagtt ttggtccat ttccactcc gatatggat 
      aaaaattac ccaggtttt caaataacc tattcagtg 
      gtcgaagga atgccagga tgtggtggt gtttatacg 
      agatctcaa gacgaaatt cgttctccg atgtataac 
      ggaaattat ccaaatgat attcagtgt gaatataaa 
      atccagctg acgacgaaa agtcgaata aaattgact 
      tttctgtcg tttgatgtt gaggattcg gaaaactgt 
      caattcgat tatgttgca atttatgct ggtaccaca 
      gtagatgct cctcttatt ggaaaatat tgtggccaa 
      accgttcct tcaccatac atcaccgaa aaaagcgaa 
      attttaata gtgtttaag actgattgg gccactgct 
      ggtgaaggc ttcgctctt cgatacgaa acagtttgt 
      ggaggcaca tttacaaca ccttcaggt gttattcaa 
      tctccaggt tatcccaaa aactacgac cataatcta 
      gaatgtatc ttcgaaatt gtccaaccc cttggaaat 
      attattaaa ttaaacatt gttgatttg gatttggag 
      agtaatact tatccagat tgcgtttac gattttttg 
      gaggtaagg gatggacat aacagcaat tctacttta 
      attgggact tattgtgca aacaaagtg cctttgatt 
      gtttcatcg tacaattac ttatggatc aaatttcaa 
      tccgatacc aataacaat ggtaaaggc tttagggca 
      aattataca accactaat ataggctgt ggaggcata 
      atgaaagaa aaatcagga actttttca accccaaag 
      tatccccaa gtctaccct tctggaaca atttgcaaa 
      tggataatt tcagcacca cctgggaac gtgattcaa 
      gtaacttgg atgaatttt cagatcgaa ggcagtttt 
      gattgcact tacgatgct gtggaaatt tttgataat 
      aatacagag actggagaa ggaacatca attggaaaa 
      ttctgtgga caacacgta cctccagca atgttaagc 
      acttcaaat ctaatcaca gtcattttt cattcggat 
      caaactaat aacatgaat ggttttgtg gcttcctat 
      agcactcta ctagaatcg acagtatgt cgtggaaat 
      tactactca aatgctggt gtaattaaa tctcccgga 
      taccccaac aattacccc ctcaatatc gaatgcact 
      tggacaatt caagttaag ccgggtcat caaattttg 
      ctaaatgtg actgatttt caactcgaa cactacacg 
      acgtgtcga tacgattgg ttggaaatt cgcaatgga 
      ggaagctca atgtctcca ctcttgggt agattttgt 
      aacactgat attccgaaa atgattcca tcacacgct 
      aataaactc tttatccgg tttaaatca gatatgtca 
      aaatcggaa aaaggtttc aaaattacg tgggaagct 
      acagccact ggatgtggt ggggtttta aatagccca 
      actggatcc ataatatca ccaaattat ccagaaccc 
      tataatttg aatgctgaa tgtatttgg aaaatttcg 
      ataagtgct ggatcactt attcagttg gttttctcc 
      gatttggat cttgaggaa catagcgaa tgcgcactg 
      gattatgtt gagattttg gatggacca tctttgaac 
      tccaaatct ttgggcaga ttctgtttg caccaccct 
      tcattcctc cgatcgtca ggaaacacc ttaactgta 
      attttccga tctgatctc agtcgaaat ggacgagga 
      ttccactta caatacatc accatgtgc aacaacacc 
      ttgaagggt ttcagaggc gtgatcgaa agcccaaac 
      tttccaaat aattacccg aattcagta aattgcaac 
      tgggagata aaagtcgcc gataaaaac aaaataaac 
      atctcgttt tcacacttt gacttggaa aaagttttg 
      gtgcaaaat gtaccaagc aataactct gattcagac 
      aagtgtctt tttgactac gtggaggtt ttatacgtc 
      gaacccgtt gaagaatac gaagaagaa gggccgttt 
      caaaaatat ggaatttat tgtggggat aaaatccca 
      cctttgatt acgttgaat tcagatcat gctaaaatt 
      cgcttcctc tccgataat cttcttttt ggaaatggg 
      tttagatta gagtggcaa ttggagggt tgtggagat 
      attttaaca caccctaat ggcactatt ttctcccct 
      aattatccc agatcgtat cccccttcg attgaatgc 
      aactggaaa atccaggtt gattttggc agcaatgtt 
      gaaattact tttcataaa attgaaatt gaaaaaact 
      tacatttgt catttggat tatattaaa ttgtacaat 
      ggtgaagat gaaacgtat ccagaaatt gcgacgctt 
      tgtcaccag aataaaccg atcacgttg aggagttct 
      ggaaatttt ttgtttgtt aagtttaag gcggattca 
      tcggttcag ggaatggga ttctatgcc aattacact 
      agcacacct acaaaatgt ggtgggaaa tacaccgca 
      gacagtgcc ttcatcatg tcgccaaat tacccagaa 
      aactacaac aaaaactca acttgtggc tacgaaatt 
      caagttgga gaaggtttc cgaatcgaa ctaaaattc 
      caagacttt gacctctac gcccccaac agactcaac 
      tgtacttca aataacatc agttacgtg aaaatttac 
      gatggtcca accatcgac ttccccctc ttagccaaa 
      atttgcaat aaaaacgct cccaacaaa accatccta 
      accacaact aaccaaatg tacatcgaa atggtgtca 
      cagacagac atggcatca aaaggcttt ctagccaag 
      tatgagaaa gcttgtggt gctcgaatt gtgacgagt 
      ggaacaggg catattcga attttttcg cacgatttg 
      gactacgat gagagtaat tgcacgtgg actataatt 
      gcgaaagat ttgactaaa cacattacg ttaactgtc 
      ttatatctg gattcgttg aatggctac tgcgacgaa 
      aatattaaa attttgaag gtttacaat ggggagagt 
      tcggaagcg cctcttttg ggcgaatat tgtcagaaa 
      attcctcca actttgaca agtgatggt tctgcaatg 
      catgttgtt gttgactcc aacactgtt ctttatgct 
      acttattca gtgttcgat tcggtgtgt ggagggact 
      ctaacatca gttgctggg tactttgct tctcctggt 
      taccccaaa aagtaccca gttgacacc gaatgcgaa 
      tggacgatt gatgtaaca gaaggtaat cacgtttcg 
      ctcagtttt agctacttt ggattggtg gaatcagac 
      cactgcaat accgactat ttggagata cgacaaaat 
      aatggcact ggtaaactt ttggggacg ttttgtggt 
      agtgatctt cctacaaat atcacgaac gaaggcaat 
      ttgtggatt tttttgaaa acaagtcgt tccgatggt 
      caagtcaag ggtttttac gccgaatac gtcttaaac 
      cacgagaac gaactaagt gggccaaga ggtcgcata 
      gtctcgccc ctctactac caaaactac cccttttat 
      ggtgacttc tcctggacg attactgtg gcgtttggc 
      aaacgcata ttactaacc ttcaaagat attctactg 
      gaaaacgga gacagcatg tgtttctca tcgatttac 
      atttatgac ggttcaact gatgaggca ccaagtctt 
      ggtgaattt tgtggctcc actcttcct gacgcgatc 
      aaaactagc tctaatatt gttttgatt aaattcagt 
      ggttatagc acgtccaga tctttgacg aaatttatt 
      attgaatgg ttggagatt gaatcggtt gttaacact 
      aacaaacca tcaccgaaa ccaaatggg tgtggtacc 
      aaagaacca atcgcttta aacgaattg aagaattat 
      acaacaatc acatctcca ggttttcca aacggttat 
      gcaccaaat cttcaatgc gaatggatt ttgaccaca 
      attccaatg aatagtttg aggatttat ttcagggaa 
      atggatcta caaccaact tatggtggt tgttatgcc 
      gattacgtt gaaattttt gaaaaaagg cttgataaa 
      gattggact tcggtgctt aaaacatgt ctaagcaat 
      attacttct aatccgggc atttataca acaaatatg 
      atgaaagtt gtttttaag tcagatcgt tatgggaac 
      aaaactggc ttttcagca cttgtcaga gagagttgt 
      ggaggtact ttgacggct ccgactgat tatataatt 
      tacaacaat aattcaagg agtagttat ttaagaaca 
      tacaatagc gtgtgtcaa tggaacatc actgtacgt 
      agcggtagg accatagaa ttggaattc ttatacttc 
      aacatccac catgatacc aacaaaggt tgcgacaac 
      taccttatt attcgaaat ggaaaattc ccagactct 
      ccaatcctc ggcactggt aaatattgt ggtgaccaa 
      aaaccccca acactcaaa actaccgga aacaacgct 
      tatatcaaa ttcggaggg attacgagt ttatcgaat 
      tttattata cgctaccgc gaaatatcg tacgcttgc 
      gacagtgaa ataaaacta acaaaattc gccaactca 
      acagagata cattccccc aattaccca aacatacct 
      ccaccccat gttgagtgt aactggcgc ataatggca 
      cctcctggt gaaacactt cgaattgat ttcatcgac 
      agatttgat ctaaaagtc gccaaaaat tgcacacat 
      gaatatgtg gaagttcgc gacggtgga acgaaatac 
      tcgaaaata attggaaaa tattgcgaa aaacccaac 
      tcacagttt agtaccgac aatatgatg ttcgtgagg 
      tttttcacc gaattgaat gatccaagt aatggtttt 
      aaagcaaga attgttttg gcaaactgt ggaggaagt 
      atccattct actactggt cagatccag tctccgttt 
      ttcaacatt ccttcgaaa tatccagtt ggagttaac 
      tgtacttgg catttaatt tcaccacag aatcacaat 
      atggatatt acatttgag aaaattgac ttggaggaa 
      gctttgaat tgtacgggt gttgaccat gtcaaaatc 
      tacgaaagg aatgagttt gacaataaa gagtacgtt 
      ttgggcaac ttttgtggt aaaaagctc cccgcaatg 
      gtccaatcc tctggaagt gaagcaata gttcacttt 
      gaaactggt aaaataaaa aaagaattg ggtggtttt 
      tcattgaaa ttcaacgca agtgctgaa gtctgtgga 
      ggtagcatc gaagcttca gaaggtacc atcaaaagt 
      ccaggatat cccaatccg cataaaatc tatcgcaga 
      tgtagttgg aacatcaaa gttcccaaa ggtcgtcga 
      gtaacactc aaaattgaa gattttggt ttagataac 
      cgattcaaa gggtcatgg attaatgga ttagtaatc 
      taccacgat gagaaattt aagggttat attagaaaa 
      attagtgca agtaacgaa attgtagag tcttcggag 
      aaccacatg cttgtgttt tactggtct ccagcgact 
      atgggtaga ggattcaag gcgaaattt acctctaac 
      aacccatct gtctgtgac ggcgatttc aaccaagac 
      agtggaaca ataacccaa ccaaacatc gactatacg 
      tactcttgt acatacgtt cgcgacaaa ccttccaat 
      gctggaact ttagcttta acacttacc acaacaaca 
      aatacaacg attccatca aatgtaaag aactgtctt 
      acttacatt gcagctttc aaaattgaa acagtcaat 
      ggtaaaaaa gaactgttc tctactaat tgtgttccg 
      ggaacatca gtggtcaga atcccacat tcaaaaata 
      caattaagt gttgtgcaa tactcttct cgaaatcaa 
      gttaacttc actctttcc tataacact taccaatgt 
      ggaggtatt ataacccga caaaccggt gaaataacg 
      agtccaggt tatccgaac aaaaactcc aagtcgatg 
      gagtgtagc tggctttta cagttacct aaagaacaa 
      acgatttca ctaactttt gactcttta aatctagga 
      agcaactgt gagaagagt tatatagat atttacaat 
      ggcgattcg cccaaaagt ccaaaaatc gacaggtat 
      tgtcaaagt aacaagcct ggaacaatt tcatcacaa 
      actaacaat ttatggatc gattacaaa tttgacatc 
      gattcagac agcaaaggt tttagtctc aaatacgaa 
      cctaaaatt cagggttgt ggtggaatc tttcacgat 
      cgtgagagt attattcaa actccttcg ttccctaat 
      gattatcca aacaacgcg gaatgtttg tgggagttg 
      aggagtgtt gagggattc cacattggg cttatattc 
      acgacacgg tttcaaatt gaggaaacg gataattgt 
      gaaaatgat tttctggaa atttgggat tggcgagat 
      gatgattgg gtttcgttg ggtcgaaag tgtggtcgg 
      gaatttcca aaaactgtt aattcgaca agcaataga 
      atgaaaatt ttattcagg agtaatgaa agaataacg 
      gggaaaggg ttccgggct cagtggcag tggaactgt 
      ggtggcact ttcacagct gaccgaaaa gaacgtttc 
      atagttagt cccggattt cctcaagaa tatcaaaag 
      aacatagac tgccaatac aatatcaca tccaaacaa 
      tcacatggt cgtttcata aatattaaa ttttacaat 
      ttcgacctt gaaggaagt ggatctaat tgccgttac 
      gacaattta acactgtca cgattctgg atgaggaaa 
      gttaattat tatcacatg cccgcaact caagttttt 
      tgcggccaa aaattacca cctccaatt cgcctcggt 
      gacactctt tacattcga tttcagact gatccttgg 
      gtgcaaaaa accggtttc aaattttca tatcaactc 
      gatgactgt ggaggacag ataacttca ccaacagtt 
      atctcatct cctttgacg aaaatcgac agatccatg 
      ttcccactc tacactgga tacatgagt tgtatttgg 
      aatatcaca gcacctcca aaaaagagt atagtgatc 
      agcttccaa aaattccgt tttgagtac cgaattcgt 
      tgtcacttt gaccacgtc caaattatt gaaggacca 
      gttaacacc attgcgaag cgaatcgcg aatttgtgt 
      ggtgatttg aatcagaat cttccaact atcaagtcg 
      gaaaataac acggttttg gtggtgatg aatgttgat 
      cggtcacag aatcatgaa gggtttgag gctgaagtt 
      tattttgtt gatggacct gaagcagga tgtggaggc 
      aaaattgtg ttaaataaa actatgact ttggaagca 
      ccgaattta gccgatatg gactgttta tggacgatt 
      cagtcacaa attgatacg actgtaaaa attcatttt 
      gatgaattg aatttaccg tcaaggtgt ggtttaact 
      aatcacacg attactaat tgtagctgt gcagctctt 
      gaggtgagg gatggccac cctggcgtt ggcgactta 
      atcgaaaga ttttgtggc atcactgac acacattat 
      tcacgcgaa attacaact tcgggtaat gtgatgggc 
      ctacgactt cgaacaaga ggatccaac tcgaaagct 
      ttcaaagta acattaaca ccaatcgct tcgatttgt 
      ggtccagtt tacttaaac gtaacacaa gacgttaaa 
      actctactt tcgcccaat tatccaaac aaatatcca 
      agcgatttg agatgtagc tggatcctc aaaggcact 
      tcacgattt gttgtccgt tttattgat tttgacttg 
      aacaaagaa aggaaccag cacgttgtt aataatgtt 
      tgtggtgaa gacaaactg gaaattgtt gataatatg 
      gttaatcta ttgacaacc agtgatatg ggtccatct 
      ttcacagtc tcaggtgac agaaaattg acaactgat 
      tttttgcga tacggttat ttgaccgga acgcatcaa 
      ttttgtggt agatttgaa aatcccttc gatttctac 
      tcacaattt tcccagtta attataaaa ttcaaatcg 
      gggaatttg gacaaggat cgaggaaaa ggtttcaaa 
      attgaatat cacacaact ggttgcaac cgaaatttc 
      acctctttg caaggacgt attgtgcag aatgaccgc 
      actcaaagc aagtcttgt atccaaatt attacagtg 
      ccaaagaac tacacaatt tcgctttac ttccaagaa 
      cttacaatt tttcacagc gagggttgc caaaatgct 
      ggaattgaa atacgagat gaaaattcc caagggact 
      ttattccaa aaagtttgt ggttattat gtgccaaat 
      cctgttttt tccacgaaa aatcagttg tggattcgt 
      tcttggaac acgaacagt tttccaata tcttacgat 
      cttgtttat actgcgtca gatcaaggc agagggtgt 
      gggggaatt atctataac tataaaggg acgtttagt 
      agtccgctt catccaatg gcttataaa aatagcact 
      ccgtgtgtc tggaccctt cggacggct attggcaga 
      agaattgcc atcaaattt actgttttt gatatacaa 
      ggagactgt gcggagaat tatgtcaaa gtgactact 
      tatgagaaa aacacttcg aacgatcac aaatactgc 
      aaaaatgac gatccggct gttctttat tcgaattcc 
      cgtatgtta gttacctac cagtcttcg acaaaaaat 
      gatggttcg ggatggatc gcaaaattt gaagcagcc 
      atatctgat gaagtgatg attgttttg tcacctccg 
      ctgcaaatt gcagctctc tgctacatg ttcacagta 
      acaatcctc gttttcgtc ctaggctgt tatatctac 
      ttcctccaa agcaatcgt aaacgttgc acttgttcc 
      ctcgctgag aaagacgtc gaaatgcga aaagcgcac 
      cgaaaaagc gaaaacaac gtttacgaa gcggacaga 
      agcacaaaa gctgaacac tcttcctca ctgcatcaa 
      gtcagaatg agcgaatac gtgtccatc acaagcgat 
      tcgcaaaaa agaagttct ccttctccc ccaacaaat 
      gatcgcacg gtggacgaa accgacgat tctgcttat 
      tttttaaac gcgtatgaa actatagtt gattcattg 
      tacatttca ttgtgcaaa gtgcccaag tcgaaacaa 
      gatttatac gcgcgattt ctcaatgat ttggcgaaa 
      aaaatcaaa atgaaagac agtgcagtt tatgataag 
      ttgcaaatg tgtgggctc ttaaatgtg aatcattac 
      gaagttatt aatgaaaat gagccagga tcggaatat 
      gattcagac gattgtact tatgtgaaa tatgtgcct 
      tcagaaagt gtgaaacaa tcattaatg cgtgcgcaa 
      gcattgcac aggacacag tgccctaaa catgtgtat 
      ggtcctaac gagcggttg agaaattgt ctaaaggaa 
      cagaaagat gccaaaaac atgtcgcgg agcgaaaaa 
      cctccaatt ccaccaaaa aaggtcaga ttaaggaaa 
      aattccgag gaggatgtg atctag
    mRNA sequence
    • >TC031117 RA
      atgtgtttg tgtgtgatt ttaaaatga ttttgttgt 
      atttcgttt tttcttgtt tttacaatt ttgtgtttg 
      gagttaatt attttggac cacaaccta gaatagtga 
      ctcaggatg ggcacttaa tattttacg ccgcagttc 
      acaaaaata ttagtttgc aaacccaag gacgaggaa 
      aaatacatg ttaatggaa ttgacttat cacaggcag 
      ctaaattaa ccaatagcg cattcgatg ccatgaaaa 
      aataccaaa caaatttcg aaaacactt tcgaaaaaa 
      tctccaaat ttgagcagc ttctggacg atggtttga 
      tgcaaaaaa tcacgacat tgaatggga gaacaaacg 
      gaacaacac aagatggag taaacagtg cttctttgg 
      tgagaagcg ttcgaagac taaaccgaa gagtcaatg 
      ctttgcaaa aatctgtaa aaatgttgg ctgaccttt 
      tagaagatg atgattgtc agagtgatc cgtgcaaaa 
      acggtggaa cgtgtgaag atttattca atacttttc 
      tgtgccatt gtccacctg agtgggagg gccctgtat 
      gtgacaaag atgtgaacg aatgtgcca gatttgcag 
      gaacggatt tagggtgtc aaaatgggg ccacttgtg 
      ttaacaggc ccggctcat acgagtgtt tgtgttcgc 
      gtggttggg ttggccttc actgcagaa accgaaatg 
      ttgattgtc gccaaggtt cgaatttcg agctttgtg 
      gacacggca cgtgtatca cccaataca acgatgttg 
      gatataaat gcatttgtg acgagggat ggacaacag 
      atggcaaaa ctccagctt gtaatacag atgttaacg 
      aatgcacag gaactaaac cacactgtt cagttaatc 
      cacctgtgc agtgtatta acttacccg gaacttacc 
      gctgtggta gctgtccag ctgggtaca ctggtgatg 
      gatactatt gcacagaca ttgacgagt gcagtacat 
      caaacggtg gatgtagca ctaatccca tggtgcagt 
      gtattaata cacttggat caagatttt gcggtagtt 
      gtcctagtg gatatgtag gagacggaa tcacatgtt 
      catatcaag gtgtttgta atgtaaata atggcggtt 
      gtcatcctt tggccactt gtagaaata atccaagaa 
      ttggttcca ctttcgttg aatgtattt gtcccagtg 
      actacacag gtaacggga taggacgca atggttgtg 
      tgcgttcat tgccttcag ccccaggtc cttgcaccc 
      caaatcctt gcagacgtg gcttttgca gagttacca 
      acggaaccg attacacgt gccattgca acaggcgat 
      acgccggga aaaattgtg aaaagtttc gcgatttgt 
      gtacttcta acccttgcc aaaacggcg caacttgca 
      cttcgttct tgggcattt cttaccagt gtttgtgtc 
      catcagctt atacaggat tgcgttgcg aaacggaaa 
      aacaatctt gtggtggtc gtcttgtgg gagaaaccg 
      gaactttgg tttttcctt ctggttcgt cacaaacat 
      atagcagtg cgatgagtt gtgcctgga ttttggaaa 
      ctaatgtta ctaaagtgt taaatatca cttttaaac 
      gtttcgatg ttgaaggat cacgcgggt gtcgttatg 
      attggctac agatacatg atgggaaaa atacattgg 
      cacggtctt taggtcgtt tttgtgggc atcagttac 
      cgaatggtg gaaatattg tatcgactc ataatactg 
      tttatttgt ggtttagat cggatcatt cggttgcat 
      cagaaggat ttgagttga actggacat cgattgtac 
      cattttgtg gagatcaac tgaaaagtg taacatcgc 
      atggaataa tccaatcac ctggatcac ctggaaatt 
      acccaaata atcgtgact gctattggc cacttcaag 
      cccaaccag gaaaacgcc tcctcttcc actttttca 
      ctctaatga ttggaaaca actcagcct gtgatcacg 
      attatttag aatttattt caggaactg agcctacag 
      atccagttt tcgccaaat tttgtaata ctacacacc 
      cctctccac ttttttctc catcaggcg aagttttgg 
      tccatttcc actccgata tggataaaa attacccag 
      gttttcaaa taacctatt cagtggtcg aaggaatgc 
      caggatgtg gtggtgttt atacgagat ctcaagacg 
      aaattcgtt ctccgatgt ataacggaa attatccaa 
      atgatattc agtgtgaat ataaaatcc agctgacga 
      cgaaaagtc gaataaaat tgacttttc tgtcgtttg 
      atgttgagg attcggaaa actgtcaat tcgattatg 
      ttgcaattt atgctggta ccacagtag atgctcctc 
      ttattggaa aatattgtg gccaaaccg ttccttcac 
      catacatca ccgaaaaaa gcgaaattt taatagtgt 
      ttaagactg attgggcca ctgctggtg aaggcttcg 
      ctcttcgat acgaaacag tttgtggag gcacattta 
      caacacctt caggtgtta ttcaatctc caggttatc 
      ccaaaaact acgaccata atctagaat gtatcttcg 
      aaattgtcc aaccccttg gaaatatta ttaaattaa 
      acattgttg atttggatt tggagagta atacttatc 
      cagattgcg tttacgatt ttttggagg taagggatg 
      gacataaca gcaattcta ctttaattg ggacttatt 
      gtgcaaaca aagtgcctt tgattgttt catcgtaca 
      attacttat ggatcaaat ttcaatccg ataccaata 
      acaatggta aaggcttta gggcaaatt atacaacca 
      ctaatatag gctgtggag gcataatga aagaaaaat 
      caggaactt tttcaaccc caaagtatc cccaagtct 
      acccttctg gaacaattt gcaaatgga taatttcag 
      caccacctg ggaacgtga ttcaagtaa cttggatga 
      attttcaga tcgaaggca gttttgatt gcacttacg 
      atgctgtgg aaatttttg ataataata cagagactg 
      gagaaggaa catcaattg gaaaattct gtggacaac 
      acgtacctc cagcaatgt taagcactt caaatctaa 
      tcacagtca tttttcatt cggatcaaa ctaataaca 
      tgaatggtt ttgtggctt cctatagca ctctactag 
      aatcgacag tatgtcgtg gaaattact actcaaatg 
      ctggtgtaa ttaaatctc ccggatacc ccaacaatt 
      accccctca atatcgaat gcacttgga caattcaag 
      ttaagccgg gtcatcaaa ttttgctaa atgtgactg 
      attttcaac tcgaacact acacgacgt gtcgatacg 
      attggttgg aaattcgca atggaggaa gctcaatgt 
      ctccactct tgggtagat tttgtaaca ctgatattc 
      cgaaaatga ttccatcac acgctaata aactcttta 
      tccggttta aatcagata tgtcaaaat cggaaaaag 
      gtttcaaaa ttacgtggg aagctacag ccactggat 
      gtggtgggg ttttaaata gcccaactg gatccataa 
      tatcaccaa attatccag aaccctata atttgaatg 
      ctgaatgta tttggaaaa tttcgataa gtgctggat 
      cacttattc agttggttt tctccgatt tggatcttg 
      aggaacata gcgaatgcg cactggatt atgttgaga 
      ttttggatg gaccatctt tgaactcca aatctttgg 
      gcagattct gtttgcacc acccttcat tcctccgat 
      cgtcaggaa acaccttaa ctgtaattt tccgatctg 
      atctcagtc gaaatggac gaggattcc acttacaat 
      acatcacca tgtgcaaca acaccttga agggtttca 
      gaggcgtga tcgaaagcc caaactttc caaataatt 
      acccgaatt cagtaaatt gcaactggg agataaaag 
      tcgccgata aaaacaaaa taaacatct cgttttcac 
      actttgact tggaaaaag ttttggtgc aaaatgtac 
      caagcaata actctgatt cagacaagt gtctttttg 
      actacgtgg aggttttat acgtcgaac ccgttgaag 
      aatacgaag aagaagggc cgtttcaaa aatatggaa 
      tttattgtg gggataaaa tcccacctt tgattacgt 
      tgaattcag atcatgcta aaattcgct tcctctccg 
      ataatcttc tttttggaa atgggttta gattagagt 
      ggcaattgg agggttgtg gagatattt taacacacc 
      ctaatggca ctattttct cccctaatt atcccagat 
      cgtatcccc cttcgattg aatgcaact ggaaaatcc 
      aggttgatt ttggcagca atgttgaaa ttacttttc 
      ataaaattg aaattgaaa aaacttaca tttgtcatt 
      tggattata ttaaattgt acaatggtg aagatgaaa 
      cgtatccag aaattgcga cgctttgtc accagaata 
      aaccgatca cgttgagga gttctggaa attttttgt 
      ttgttaagt ttaaggcgg attcatcgg ttcagggaa 
      tgggattct atgccaatt acactagca cacctacaa 
      aatgtggtg ggaaataca ccgcagaca gtgccttca 
      tcatgtcgc caaattacc cagaaaact acaacaaaa 
      actcaactt gtggctacg aaattcaag ttggagaag 
      gtttccgaa tcgaactaa aattccaag actttgacc 
      tctacgccc ccaacagac tcaactgta cttcaaata 
      acatcagtt acgtgaaaa tttacgatg gtccaacca 
      tcgacttcc ccctcttag ccaaaattt gcaataaaa 
      acgctccca acaaaacca tcctaacca caactaacc 
      aaatgtaca tcgaaatgg tgtcacaga cagacatgg 
      catcaaaag gctttctag ccaagtatg agaaagctt 
      gtggtgctc gaattgtga cgagtggaa cagggcata 
      ttcgaattt tttcgcacg atttggact acgatgaga 
      gtaattgca cgtggacta taattgcga aagatttga 
      ctaaacaca ttacgttaa ctgtcttat atctggatt 
      cgttgaatg gctactgcg acgaaaata ttaaaattt 
      tgaaggttt acaatgggg agagttcgg aagcgcctc 
      ttttgggcg aatattgtc agaaaattc ctccaactt 
      tgacaagtg atggttctg caatgcatg ttgttgttg 
      actccaaca ctgttcttt atgctactt attcagtgt 
      tcgattcgg tgtgtggag ggactctaa catcagttg 
      ctgggtact ttgcttctc ctggttacc ccaaaaagt 
      acccagttg acaccgaat gcgaatgga cgattgatg 
      taacagaag gtaatcacg tttcgctca gttttagct 
      actttggat tggtggaat cagaccact gcaataccg 
      actatttgg agatacgac aaaataatg gcactggta 
      aacttttgg ggacgtttt gtggtagtg atcttccta 
      caaatatca cgaacgaag gcaatttgt ggatttttt 
      tgaaaacaa gtcgttccg atggtcaag tcaagggtt 
      tttacgccg aatacgtct taaaccacg agaacgaac 
      taagtgggc caagaggtc gcatagtct cgcccctct 
      actaccaaa actacccct tttatggtg acttctcct 
      ggacgatta ctgtggcgt ttggcaaac gcatattac 
      taaccttca aagatattc tactggaaa acggagaca 
      gcatgtgtt tctcatcga tttacattt atgacggtt 
      caactgatg aggcaccaa gtcttggtg aattttgtg 
      gctccactc ttcctgacg cgatcaaaa ctagctcta 
      atattgttt tgattaaat tcagtggtt atagcacgt 
      ccagatctt tgacgaaat ttattattg aatggttgg 
      agattgaat cggttgtta acactaaca aaccatcac 
      cgaaaccaa atgggtgtg gtaccaaag aaccaatcg 
      ctttaaacg aattgaaga attatacaa caatcacat 
      ctccaggtt ttccaaacg gttatgcac caaatcttc 
      aatgcgaat ggattttga ccacaattc caatgaata 
      gtttgagga tttatttca gggaaatgg atctacaac 
      caacttatg gtggttgtt atgccgatt acgttgaaa 
      tttttgaaa aaaggcttg ataaagatt ggacttcgg 
      tgcttaaaa catgtctaa gcaatatta cttctaatc 
      cgggcattt atacaacaa atatgatga aagttgttt 
      ttaagtcag atcgttatg ggaacaaaa ctggctttt 
      cagcacttg tcagagaga gttgtggag gtactttga 
      cggctccga ctgattata taatttaca acaataatt 
      caaggagta gttatttaa gaacataca atagcgtgt 
      gtcaatgga acatcactg tacgtagcg gtaggacca 
      tagaattgg aattcttat acttcaaca tccaccatg 
      ataccaaca aaggttgcg acaactacc ttattattc 
      gaaatggaa aattcccag actctccaa tcctcggca 
      ctggtaaat attgtggtg accaaaaac ccccaacac 
      tcaaaacta ccggaaaca acgcttata tcaaattcg 
      gagggatta cgagtttat cgaatttta ttatacgct 
      accgcgaaa tatcgtacg cttgcgaca gtgaaataa 
      aactaacaa aattcgcca actcaacag agatacatt 
      cccccaatt acccaaaca tacctccac cccatgttg 
      agtgtaact ggcgcataa tggcacctc ctggtgaaa 
      cacttcgaa ttgatttca tcgacagat ttgatctaa 
      aagtcgcca aaaattgca cacatgaat atgtggaag 
      ttcgcgacg gtggaacga aatactcga aaataattg 
      gaaaatatt gcgaaaaac ccaactcac agtttagta 
      ccgacaata tgatgttcg tgaggtttt tcaccgaat 
      tgaatgatc caagtaatg gttttaaag caagaattg 
      ttttggcaa actgtggag gaagtatcc attctacta 
      ctggtcaga tccagtctc cgtttttca acattcctt 
      cgaaatatc cagttggag ttaactgta cttggcatt 
      taatttcac cacagaatc acaatatgg atattacat 
      ttgagaaaa ttgacttgg aggaagctt tgaattgta 
      cgggtgttg accatgtca aaatctacg aaaggaatg 
      agtttgaca ataaagagt acgttttgg gcaactttt 
      gtggtaaaa agctccccg caatggtcc aatcctctg 
      gaagtgaag caatagttc actttgaaa ctggtaaaa 
      taaaaaaag aattgggtg gtttttcat tgaaattca 
      acgcaagtg ctgaagtct gtggaggta gcatcgaag 
      cttcagaag gtaccatca aaagtccag gatatccca 
      atccgcata aaatctatc gcagatgta gttggaaca 
      tcaaagttc ccaaaggtc gtcgagtaa cactcaaaa 
      ttgaagatt ttggtttag ataaccgat tcaaagggt 
      catggatta atggattag taatctacc acgatgaga 
      aatttaagg gttatatta gaaaaatta gtgcaagta 
      acgaaattg tagagtctt cggagaacc acatgcttg 
      tgttttact ggtctccag cgactatgg gtagaggat 
      tcaaggcga aatttacct ctaacaacc catctgtct 
      gtgacggcg atttcaacc aagacagtg gaacaataa 
      cccaaccaa acatcgact atacgtact cttgtacat 
      acgttcgcg acaaacctt ccaatgctg gaactttag 
      ctttaacac ttaccacaa caacaaata caacgattc 
      catcaaatg taaagaact gtcttactt acattgcag 
      ctttcaaaa ttgaaacag tcaatggta aaaaagaac 
      tgttctcta ctaattgtg ttccgggaa catcagtgg 
      tcagaatcc cacattcaa aaatacaat taagtgttg 
      tgcaatact cttctcgaa atcaagtta acttcactc 
      tttcctata acacttacc aatgtggag gtattataa 
      cccgacaaa ccggtgaaa taacgagtc caggttatc 
      cgaacaaaa actccaagt cgatggagt gtagctggc 
      ttttacagt tacctaaag aacaaacga tttcactaa 
      cttttgact ctttaaatc taggaagca actgtgaga 
      agagttata tagatattt acaatggcg attcgccca 
      aaagtccaa aaatcgaca ggtattgtc aaagtaaca 
      agcctggaa caatttcat cacaaacta acaatttat 
      ggatcgatt acaaatttg acatcgatt cagacagca 
      aaggtttta gtctcaaat acgaaccta aaattcagg 
      gttgtggtg gaatctttc acgatcgtg agagtatta 
      ttcaaactc cttcgttcc ctaatgatt atccaaaca 
      acgcggaat gtttgtggg agttgagga gtgttgagg 
      gattccaca ttgggctta tattcacga cacggtttc 
      aaattgagg aaacggata attgtgaaa atgattttc 
      tggaaattt gggattggc gagatgatg attgggttt 
      cgttgggtc gaaagtgtg gtcgggaat ttccaaaaa 
      ctgttaatt cgacaagca atagaatga aaattttat 
      tcaggagta atgaaagaa taacgggga aagggttcc 
      gggctcagt ggcagtgga actgtggtg gcactttca 
      cagctgacc gaaaagaac gtttcatag ttagtcccg 
      gatttcctc aagaatatc aaaagaaca tagactgcc 
      aatacaata tcacatcca aacaatcac atggtcgtt 
      tcataaata ttaaatttt acaatttcg accttgaag 
      gaagtggat ctaattgcc gttacgaca atttaacac 
      tgtcacgat tctggatga ggaaagtta attattatc 
      acatgcccg caactcaag ttttttgcg gccaaaaat 
      taccacctc caattcgcc tcggtgaca ctctttaca 
      ttcgatttc agactgatc cttgggtgc aaaaaaccg 
      gtttcaaat tttcatatc aactcgatg actgtggag 
      gacagataa cttcaccaa cagttatct catctcctt 
      tgacgaaaa tcgacagat ccatgttcc cactctaca 
      ctggataca tgagttgta tttggaata tcacagcac 
      ctccaaaaa agagtatag tgatcagct tccaaaaat 
      tccgttttg agtaccgaa ttcgttgtc actttgacc 
      acgtccaaa ttattgaag gaccagtta acaccattg 
      cgaagcgaa tcgcgaatt tgtgtggtg atttgaatc 
      agaatcttc caactatca agtcggaaa ataacacgg 
      ttttggtgg tgatgaatg ttgatcggt cacagaatc 
      atgaagggt ttgaggctg aagtttatt ttgttgatg 
      gacctgaag caggatgtg gaggcaaaa ttgtgttaa 
      ataaaacta tgactttgg aagcaccga atttagccg 
      atatggact gtttatgga cgattcagt cacaaattg 
      atacgactg taaaaattc attttgatg aattgaatt 
      taccgtcaa ggtgtggtt taactaatc acacgatta 
      ctaattgta gctgtgcag ctcttgagg tgagggatg 
      gccaccctg gcgttggcg acttaatcg aaagatttt 
      gtggcatca ctgacacac attattcac gcgaaatta 
      caacttcgg gtaatgtga tgggcctac gacttcgaa 
      caagaggat ccaactcga aagctttca aagtaacat 
      taacaccaa tcgcttcga tttgtggtc cagtttact 
      taaacgtaa cacaagacg ttaaaactc tactttcgc 
      ccaattatc caaacaaat atccaagcg atttgagat 
      gtagctgga tcctcaaag gcacttcac gatttgttg 
      tccgtttta ttgattttg acttgaaca aagaaagga 
      accagcacg ttgttaata atgtttgtg gtgaagaca 
      aactggaaa ttgttgata atatggtta atctattga 
      caaccagtg atatgggtc catctttca cagtctcag 
      gtgacagaa aattgacaa ctgattttt tgcgatacg 
      gttatttga ccggaacgc atcaatttt gtggtagat 
      ttgaaaatc ccttcgatt tctactcac aattttccc 
      agttaatta taaaattca aatcgggga atttggaca 
      aggatcgag gaaaaggtt tcaaaattg aatatcaca 
      caactggtt gcaaccgaa atttcacct ctttgcaag 
      gacgtattg tgcagaatg accgcactc aaagcaagt 
      cttgtatcc aaattatta cagtgccaa agaactaca 
      caatttcgc tttacttcc aagaactta caatttttc 
      acagcgagg gttgccaaa atgctggaa ttgaaatac 
      gagatgaaa attcccaag ggactttat tccaaaaag 
      tttgtggtt attatgtgc caaatcctg ttttttcca 
      cgaaaaatc agttgtgga ttcgttctt ggaacacga 
      acagttttc caatatctt acgatcttg tttatactg 
      cgtcagatc aaggcagag ggtgtgggg gaattatct 
      ataactata aagggacgt ttagtagtc cgcttcatc 
      caatggctt ataaaaata gcactccgt gtgtctgga 
      cccttcgga cggctattg gcagaagaa ttgccatca 
      aatttactg tttttgata tacaaggag actgtgcgg 
      agaattatg tcaaagtga ctacttatg agaaaaaca 
      cttcgaacg atcacaaat actgcaaaa atgacgatc 
      cggctgttc tttattcga attcccgta tgttagtta 
      cctaccagt cttcgacaa aaaatgatg gttcgggat 
      ggatcgcaa aatttgaag cagccatat ctgatgaag 
      tgatgattg ttttgtcac ctccgctgc aaattgcag 
      ctctctgct acatgttca cagtaacaa tcctcgttt 
      tcgtcctag gctgttata tctacttcc tccaaagca 
      atcgtaaac gttgcactt gttccctcg ctgagaaag 
      acgtcgaaa tgcgaaaag cgcaccgaa aaagcgaaa 
      acaacgttt acgaagcgg acagaagca caaaagctg 
      aacactctt cctcactgc atcaagtca gaatgagcg 
      aatacgtgt ccatcacaa gcgattcgc aaaaaagaa 
      gttctcctt ctcccccaa caaatgatc gcacggtgg 
      acgaaaccg acgattctg cttattttt taaacgcgt 
      atgaaacta tagttgatt cattgtaca tttcattgt 
      gcaaagtgc ccaagtcga aacaagatt tatacgcgc 
      gatttctca atgatttgg cgaaaaaaa tcaaaatga 
      aagacagtg cagtttatg ataagttgc aaatgtgtg 
      ggctcttaa atgtgaatc attacgaag ttattaatg 
      aaaatgagc caggatcgg aatatgatt cagacgatt 
      gtacttatg tgaaatatg tgccttcag aaagtgtga 
      aacaatcat taatgcgtg cgcaagcat tgcacagga 
      cacagtgcc ctaaacatg tgtatggtc ctaacgagc 
      ggttgagaa attgtctaa aggaacaga aagatgcca 
      aaaacatgt cgcggagcg aaaaacctc caattccac 
      caaaaaagg tcagattaa ggaaaaatt ccgaggagg 
      atgtgatct agaaggagg cgaaaataa ctcgagtga 
      aaaatagat tgcaaactg tctcggttt aaaattgtt 
      tgagaaaaa ttgaatttt catgacaaa catttgaaa 
      aagtgttga aaggtgtaa gtttctttt aatgctgcg 
      aattcgaaa aatgtattg aagagaatt aaattacct 
      tcaaaagg
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