Status
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Gene information
General information
TC008485 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11377980..11381218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008485
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02779

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008485 RA
        MSWVDLPPP PGSKVKHGK TTIKIVNKY NYHYPGFYT 
        IPVQHPHYH GYYQTYSTY HYTTSDTGS GILGFYLGY 
        KLGKLSTPT YSHESFYDG YRPRYDHYT IHHYYHNAQ 
        AIPKRQEIV SNAIIGCVG DSATVCPAN TTSLCTSDG 
        TLMCVVAAT ALITCKDAN NQMVSCTSS TMSCVNNTA 
        PECVGTNKS NVVVNIPCL STADIHGDI KFVNNTIVK 
        NTVPPNNTT TEGMSYGST YGSVPLINN NQTLNGTTN 
        RANLNGTEG EMKYINNNE TLAISNGTI NGTEVIPGS 
        IKYVNNNET LATLNGTTN LNSTEGGSG GMKYVDNNE 
        TLSLSTTPS STTSEYWKW KRSVPQASQ QFCVTIVAS 
        PAKREPTQG EEFLAEANS LLRKFIETA WNL
      CDS sequence
      • >TC008485 RA
        atgtcttgg gttgactta ccacctcct cctggatca 
        aaagttaaa catgggaaa acaacaatt aaaatcgtc 
        aacaaatac aattaccac taccccggc ttttacaca 
        attcccgtt caacaccca cactaccat gggtactac 
        caaacttac tcaacctac cactacact acttctgat 
        accggaagt ggtattctt ggattttac ttggggtat 
        aagttgggt aaattatca acacccaca tactcacac 
        gagtcattt tatgatgga taccgccca cggtacgac 
        cattacaca atccatcat tactaccat aacgcccaa 
        gccattcct aaaagacaa gaaattgtt tccaatgct 
        attattggt tgtgttgga gatagtgct actgtatgt 
        ccagctaat acaacatca ctttgtacc agcgatggt 
        actttgatg tgtgtggtt gctgctaca gctctgatt 
        acttgtaaa gacgccaac aatcaaatg gtaagttgt 
        acgagtagt accatgtct tgcgtcaac aatacagca 
        ccggaatgt gttggtact aacaagtct aatgtcgtg 
        gtgaatata ccatgctta tcgacggcc gatattcac 
        ggtgatatc aaatttgtc aacaatacc attgtaaaa 
        aatacggta ccaccaaat aataccacc acagaaggg 
        atgagctat ggtagtact tatggttct gtgccttta 
        attaacaat aatcaaaca cttaatggt accacaaat 
        cgtgcaaat ctcaatggg actgaagga gaaatgaaa 
        tatatcaac aacaatgaa actttagcc ataagtaat 
        ggtaccata aatggtact gaagtaatt ccaggaagc 
        ataaaatat gttaataat aacgaaact ctggctaca 
        cttaatggt actacaaat cttaatagt actgaaggc 
        ggttcagga ggaatgaaa tatgttgat aataacgaa 
        acactgtct ctttctaca actccaagt agtaccact 
        tcggagtac tggaaatgg aagcggtca gtacctcaa 
        gcatctcaa cagttttgt gtcacaata gtggcatca 
        cctgcgaag agagagcca acacaagga gaagaattt 
        ttggcagaa gccaacagt ctgttgcga aaattcatc 
        gaaactgct tggaatctc tag
      mRNA sequence
      • >TC008485 RA
        aatttgaaa cagctaata gatacaata gaagttaaa 
        aagtttcta atgtgaccc ctaagagaa tcagtctct 
        tacgacgac gagattcaa tcaactgtg ggccctgat 
        aattggggg tcaggactc caagcactt gcttaccaa 
        tcaaaaact acaaggagg agcagaacc acaccacga 
        agaacaaac cctccaaca acaccacaa agggttaga 
        actacagcc cggagaaca aaccctcca acaacacca 
        cgaagaact acccaacga tgggtggag gtaacaacc 
        agaagaccc acaacaacc agaaggtcc acaacaacc 
        agaaggtct acaactccc agacgtacc caaccaaca 
        acaaggcct tgggtaact gttacaaca agaaagact 
        acaactacc agacgttgg ataagtgtt acaactaga 
        aagcctaca actacaaga cgtacccaa cccccaact 
        acaccccga cctcgactg actaccaca acccctaga 
        cgaacatac ccaactcca actccaacc ccttggtgg 
        aaaactaaa agtaccacc agatggaca ccacccaca 
        agaaccgtt acaacacga aggcctaaa acgaccact 
        aaaccatat tacaagaaa gacatgtct tgggttgac 
        ttaccacct cctcctgga tcaaaagtt aaacatggg 
        aaaacaaca attaaaatc gtcaacaaa tacaattac 
        cactacccc ggcttttac acaattccc gttcaacac 
        ccacactac catgggtac taccaaact tactcaacc 
        taccactac actacttct gataccgga agtggtatt 
        cttggattt tacttgggg tataagttg ggtaaatta 
        tcaacaccc acatactca cacgagtca ttttatgat 
        ggataccgc ccacggtac gaccattac acaatccat 
        cattactac cataacgcc caagccatt cctaaaaga 
        caagaaatt gtttccaat gctattatt ggttgtgtt 
        ggagatagt gctactgta tgtccagct aatacaaca 
        tcactttgt accagcgat ggtactttg atgtgtgtg 
        gttgctgct acagctctg attacttgt aaagacgcc 
        aacaatcaa atggtaagt tgtacgagt agtaccatg 
        tcttgcgtc aacaataca gcaccggaa tgtgttggt 
        actaacaag tctaatgtc gtggtgaat ataccatgc 
        ttatcgacg gccgatatt cacggtgat atcaaattt 
        gtcaacaat accattgta aaaaatacg gtaccacca 
        aataatacc accacagaa gggatgagc tatggtagt 
        acttatggt tctgtgcct ttaattaac aataatcaa 
        acacttaat ggtaccaca aatcgtgca aatctcaat 
        gggactgaa ggagaaatg aaatatatc aacaacaat 
        gaaacttta gccataagt aatggtacc ataaatggt 
        actgaagta attccagga agcataaaa tatgttaat 
        aataacgaa actctggct acacttaat ggtactaca 
        aatcttaat agtactgaa ggcggttca ggaggaatg 
        aaatatgtt gataataac gaaacactg tctctttct 
        acaactcca agtagtacc acttcggag tactggaaa 
        tggaagcgg tcagtacct caagcatct caacagttt 
        tgtgtcaca atagtggca tcacctgcg aagagagag 
        ccaacacaa ggagaagaa tttttggca gaagccaac 
        agtctgttg cgaaaattc atcgaaact gcttggaat 
        ctctagtgt gaaagttgc tttaataaa aattaccct 
        cgattaaga ccagctgct ttacgttat gagctaatt 
        gcattaaaa attgattct gaacacttt gctgaaagc 
        gagataaat tttcgcttg cagacacaa aaattttat 
        gttcgctgc attccagca accggaact tatttcact 
        tatacgttt tctgttatt ccggcaaaa attccggtt 
        cggtgatgt gattaagtc aggatctga aattaatct 
        ccaaacgca accgctgct ccttaacaa tgatagttc 
        ccccaactt taactaaca ctattgtat agtttgtgc 
        caaattcag acttcgaag ttgaattat taaattagc 
        tttcgaaaa atgcaatga cttgtccct tattaattc 
        aatttaaaa ctcgaacct accgttgca aaagttttg 
        atgagaata acttcctcc tctacaagg gtttagctc 
        gttaaatga ctaagaaaa ttggactag ggctttgcc 
        tctgaatta atcacattg gttgagtta gcacttaaa 
        aataggttt atcactcgg cggaatttt caaacgtaa 
        acgcgaata ttttgtgac cctgaaact ggaacaagg 
        ctgaaagtc gtgtaattt cagcacgac acccttgaa 
        cgctagttt cataaactc gaatggaat aaacatctc 
        tcaccaca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY