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Gene information
General information
TC032913
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008469,TC008470
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11097456..11103130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032913
    iBeetle screen iB_02777

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032913 RA
      MNDNAKLYS SVGQLQKQG IAETLQKYI KLVRFKDKI 
      KVLDVGCAD GGLTSEILV PNLPPDFDE IIGMDISPK 
      MVTYAREKL KANAKFSFV QFDITSEQI PEKFYEYFD 
      NIFSFYSFN WISEHKHPQ ALKNLYKML KPGGYIFLV 
      IISTSQVFD VYETMSQSE KWAPFVKNV RETVSLYHF 
      IDQPQPKLN NFLKSAGFD CHLCTLEEK CYTFENLAH 
      FWEWAVSIN PFYKKIPEN KLQSYQEDY LNEFRKHKN 
      VFIEVSNRE EKIRKPYTT LVACASKPE EVQAFVQDL 
      VVKMNDNAE LYSDFGDLQ RRDADYILK KYSNYLKFG 
      KNAKVLDIG TADGTLTSQ ILFPKLPQD LEKLIGFDI 
      AENMVNYAK KKCQDLPKL DFVQMDITC EKIPLKYYQ 
      SFDNIFSFY CLNWVPEES FPQTMKNIY DLLKPGGQA 
      FITIVGNSP LFDFLKRTS LKEKWAPIM SNVDQYLSI 
      FHKLEHPEV KLEKFVTDA GFESKLCTV EHLSYVFPN 
      FTYWWDWAM SINPFIKNL SENEIDLYR EDYMKEVKR 
      HKSVTIQDG EKIAIVYTI LVVCASKP
    CDS sequence
    • >TC032913 RA
      atgaacgac aacgcgaaa ctttattca agcgtcggc 
      cagttgcaa aaacaaggc atcgctgaa actttgcaa 
      aaatacata aaacttgtt cgtttcaaa gacaaaata 
      aaagtgtta gatgttggg tgtgctgat ggtggtctc 
      acttccgaa attcttgtg ccaaatttg ccgccagat 
      tttgacgaa attatcgga atggacatc tccccaaaa 
      atggtaact tacgccaga gaaaagctt aaagcaaat 
      gcaaaattt agtttcgtt caatttgat atcacttcg 
      gaacaaatt cccgagaaa ttttacgaa tatttcgac 
      aacattttc tcgttttat agctttaat tggatttct 
      gaacacaaa catcctcaa gcactcaaa aatttgtac 
      aaaatgcta aaacccggc ggttacatt tttctagtg 
      attatttca accagccaa gttttcgac gtttatgaa 
      accatgtcc cagtctgaa aagtgggcc ccatttgtg 
      aaaaatgtg agagaaacg gtatcgctc tatcacttc 
      atcgaccaa ccacaaccg aaactgaac aatttcctg 
      aaaagtgca ggttttgat tgtcattta tgcacgctg 
      gaagagaaa tgttacaca tttgagaat ttagcgcac 
      ttttgggaa tgggcggtt tctattaat ccattttat 
      aaaaaaatt ccggaaaat aaactgcag tcgtaccaa 
      gaagactat ttgaacgaa tttaggaaa cacaaaaat 
      gtttttatc gaagtctca aatcgggaa gaaaaaatt 
      cggaagcca tacaccact cttgtggct tgtgcctca 
      aagcccgag gaagtgcaa gcgtttgtg caagattta 
      gttgttaaa atgaacgac aacgctgaa ctttactcg 
      gatttcggt gacttgcaa agacgcgac gccgattac 
      attttgaaa aaatattca aactacctc aaatttggg 
      aaaaatgct aaggtgctg gacattggg acggctgat 
      ggcaccctc acatcacaa attttattc ccaaaacta 
      ccccaagat ttggaaaaa ttgatcggg tttgatatc 
      gccgaaaac atggtaaat tacgctaag aaaaaatgc 
      caggatcta ccgaaactt gactttgtt caaatggac 
      atcacttgc gaaaaaatc cccttgaaa tattaccaa 
      tccttcgac aatattttt tcgttttat tgcctcaat 
      tgggtccca gaggaaagc tttccccaa acgatgaaa 
      aatatttac gacttgcta aaaccagga ggccaagct 
      tttattact attgttgga aatagccca ctttttgat 
      tttctcaaa aggacttct ttgaaggaa aaatgggct 
      ccaattatg tcaaatgtt gatcagtac ttgtcgatt 
      tttcacaaa ttggagcat cccgaagtt aaattagaa 
      aaattcgtg acggatgct ggttttgag agcaaattg 
      tgcaccgtt gaacatttg agttacgtt tttccgaat 
      tttacatat tggtgggat tgggcaatg tcgatcaat 
      ccatttatc aaaaatctc tccgaaaat gagatcgat 
      ttgtaccgc gaagactac atgaaagag gtgaagaga 
      cacaagtcg gttacgatt caagacggg gagaaaatt 
      gcaattgtc tacacaatt cttgtcgtt tgtgcttca 
      aaaccatga
    mRNA sequence
    • >TC032913 RA
      agcaatgaa cgacaacgc gaaacttta ttcaagcgt 
      cggccagtt gcaaaaaca aggcatcgc tgaaacttt 
      gcaaaaata cataaaact tgttcgttt caaagacaa 
      aataaaagt gttagatgt tgggtgtgc tgatggtgg 
      tctcacttc cgaaattct tgtgccaaa tttgccgcc 
      agattttga cgaaattat cggaatgga catctcccc 
      aaaaatggt aacttacgc cagagaaaa gcttaaagc 
      aaatgcaaa atttagttt cgttcaatt tgatatcac 
      ttcggaaca aattcccga gaaatttta cgaatattt 
      cgacaacat tttctcgtt ttatagctt taattggat 
      ttctgaaca caaacatcc tcaagcact caaaaattt 
      gtacaaaat gctaaaacc cggcggtta catttttct 
      agtgattat ttcaaccag ccaagtttt cgacgttta 
      tgaaaccat gtcccagtc tgaaaagtg ggccccatt 
      tgtgaaaaa tgtgagaga aacggtatc gctctatca 
      cttcatcga ccaaccaca accgaaact gaacaattt 
      cctgaaaag tgcaggttt tgattgtca tttatgcac 
      gctggaaga gaaatgtta cacatttga gaatttagc 
      gcacttttg ggaatgggc ggtttctat taatccatt 
      ttataaaaa aattccgga aaataaact gcagtcgta 
      ccaagaaga ctatttgaa cgaatttag gaaacacaa 
      aaatgtttt tatcgaagt ctcaaatcg ggaagaaaa 
      aattcggaa gccatacac cactcttgt ggcttgtgc 
      ctcaaagcc cgaggaagt gcaagcgtt tgtgcaaga 
      tttagttgt taaaatgaa cgacaacgc tgaacttta 
      ctcggattt cggtgactt gcaaagacg cgacgccga 
      ttacatttt gaaaaaata ttcaaacta cctcaaatt 
      tgggaaaaa tgctaaggt gctggacat tgggacggc 
      tgatggcac cctcacatc acaaatttt attcccaaa 
      actacccca agatttgga aaaattgat cgggtttga 
      tatcgccga aaacatggt aaattacgc taagaaaaa 
      atgccagga tctaccgaa acttgactt tgttcaaat 
      ggacatcac ttgcgaaaa aatcccctt gaaatatta 
      ccaatcctt cgacaatat tttttcgtt ttattgcct 
      caattgggt cccagagga aagctttcc ccaaacgat 
      gaaaaatat ttacgactt gctaaaacc aggaggcca 
      agcttttat tactattgt tggaaatag cccactttt 
      tgattttct caaaaggac ttctttgaa ggaaaaatg 
      ggctccaat tatgtcaaa tgttgatca gtacttgtc 
      gatttttca caaattgga gcatcccga agttaaatt 
      agaaaaatt cgtgacgga tgctggttt tgagagcaa 
      attgtgcac cgttgaaca tttgagtta cgtttttcc 
      gaattttac atattggtg ggattgggc aatgtcgat 
      caatccatt tatcaaaaa tctctccga aaatgagat 
      cgatttgta ccgcgaaga ctacatgaa agaggtgaa 
      gagacacaa gtcggttac gattcaaga cggggagaa 
      aattgcaat tgtctacac aattcttgt cgtttgtgc 
      ttcaaaacc atgatttaa taaataaat gaatcgtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY