General information | |||
TC007154 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8413588..8425070 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC007154 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02775 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007154 RA
MISESGEST ASRTVGFTS AGEDSLFHK LKTSELLKH LLKKSGDSQ NDSVNKVAK VPPKISSGL ESVLSGAKD SDDGIMDEL GDSDGEVRN YRDNFQRLF SDESSQSNQ NDGLSEEEL PVIPDEGLS SETVDFILA EAEKNLHIQ SPPSSQVSQ DSDSYEFTE LLPVSNLAF LPQPSTSKN DVSPGATFD PTAADDSLR RSTESINTS QKSAGNSDS DSDIDNEMV LVPSDPLEW TNTHIKSWL SWCSRKFSL NPKPDFEKF PTTGKELCE LTRTDFETK AGCERTGTI LAKHIAHLR HSVTGRSTS PLNVECKVF EDDDDDEKD PYQLLNAAS SRLVAQGSG QIQLWQFLL ELLGDSSNS ACITWEGTN GEFKLTDPD EVARRWGER KSKPNMNYD KLSRALRYY YDKNIMSKV HGKRYAYKF DFHGLMAAC QAQAQGQTD VSPGYHKYQ PHQSELGAA LYPTGHATA PKIPSILPP GAQHTQTGL FPPPSYWPY SPGSFDPRG PFN
- >TC007154 RA
atgatctct gaaagtggt gaatcaacg gccagtaga accgttggg ttcacatcg gccggtgag gactcattg ttccacaaa ctgaaaact tccgaattg ctcaagcat ctgctgaaa aaatcgggt gattcacaa aatgattca gttaataag gtcgcgaaa gtaccgccg aaaatcagt tcgggactt gaatcagtg ctaagtggt gccaaagac tccgatgat gggattatg gacgagctg ggtgacagt gatggtgaa gtgcggaat tacagagac aactttcag cgacttttc agcgacgaa agctcgcag tcgaatcaa aatgacgga ctgtcggaa gaggagctt ccggttatc ccggacgaa ggtttgtcg agtgagacc gtcgatttt atactcgca gaggccgaa aaaaatcta cacattcaa tcgccaccc tctagtcaa gtgtcgcag gacagcgac agctacgaa ttcaccgaa ttactgcca gtctcaaat ttggcattt ttgccgcaa ccgtcaacc tcgaagaat gatgtctca cctggcgcc acgtttgat ccgacggct gccgatgat agtttaagg agatctaca gagagtatt aacacaagc caaaaatct gctggaaac agcgacagt gatagcgat attgataat gaaatggtt ttagttcct tccgatccc ctcgaatgg accaacacc catattaaa tcttggctg tcatggtgt tctcggaaa ttctctctg aatccgaaa cccgatttc gagaagttc cccacgacc ggaaaagaa ctatgcgaa ctaacaaga actgatttc gagaccaaa gcaggttgt gaaagaacc ggcacaatt cttgccaaa cacatcgcc catcttcgc cacagcgtc accggaaga tcgaccagt cctctgaac gtcgaatgc aaggtcttc gaagacgac gacgacgac gagaaagat ccctatcaa ctgttaaac gcggcttca agccgattg gtggcccaa ggatccggc cagatccaa ctgtggcag tttctcttg gaactgctt ggcgattcg tcgaattca gcgtgtata acgtgggaa ggcaccaat ggagaattc aagctcacc gatccggac gaagttgcc cggagatgg ggagaacga aaatctaaa ccaaatatg aactatgat aaactcagc agggcgctt aggtattac tacgacaag aacataatg tcgaaggtc cacggaaaa agatacgct tacaaattt gacttccac ggtctcatg gcagcgtgc caagcccag gcccaaggc cagactgac gtctctcca ggataccat aaatatcag ccgcaccaa agtgaatta ggagctgct ttatatccc acaggacac gccaccgct cccaaaata cccagcatc cttcctccg ggtgcccaa cacacccaa acaggatta ttcccaccg cctagctac tggccgtat tcacctgga tcgttcgat ccccgcgga cccttcaat tga
- >TC007154 RA
aaagtcgag tgcgttgca catgaaatt aaacttttc cgggaattg atgcgtgaa tcagttagc aatgatctc tgaaagtgg tgaatcaac ggccagtag aaccgttgg gttcacatc ggccggtga ggactcatt gttccacaa actgaaaac ttccgaatt gctcaagca tctgctgaa aaaatcggg tgattcaca aaatgattc agttaataa ggtcgcgaa agtaccgcc gaaaatcag ttcgggact tgaatcagt gctaagtgg tgccaaaga ctccgatga tgggattat ggacgagct gggtgacag tgatggtga agtgcggaa ttacagaga caactttca gcgactttt cagcgacga aagctcgca gtcgaatca aaatgacgg actgtcgga agaggagct tccggttat cccggacga aggtttgtc gagtgagac cgtcgattt tatactcgc agaggccga aaaaaatct acacattca atcgccacc ctctagtca agtgtcgca ggacagcga cagctacga attcaccga attactgcc agtctcaaa tttggcatt tttgccgca accgtcaac ctcgaagaa tgatgtctc acctggcgc cacgtttga tccgacggc tgccgatga tagtttaag gagatctac agagagtat taacacaag ccaaaaatc tgctggaaa cagcgacag tgatagcga tattgataa tgaaatggt tttagttcc ttccgatcc cctcgaatg gaccaacac ccatattaa atcttggct gtcatggtg ttctcggaa attctctct gaatccgaa acccgattt cgagaagtt ccccacgac cggaaaaga actatgcga actaacaag aactgattt cgagaccaa agcaggttg tgaaagaac cggcacaat tcttgccaa acacatcgc ccatcttcg ccacagcgt caccggaag atcgaccag tcctctgaa cgtcgaatg caaggtctt cgaagacga cgacgacga cgagaaaga tccctatca actgttaaa cgcggcttc aagccgatt ggtggccca aggatccgg ccagatcca actgtggca gtttctctt ggaactgct tggcgattc gtcgaattc agcgtgtat aacgtggga aggcaccaa tggagaatt caagctcac cgatccgga cgaagttgc ccggagatg gggagaacg aaaatctaa accaaatat gaactatga taaactcag cagggcgct taggtatta ctacgacaa gaacataat gtcgaaggt ccacggaaa aagatacgc ttacaaatt tgacttcca cggtctcat ggcagcgtg ccaagccca ggcccaagg ccagactga cgtctctcc aggatacca taaatatca gccgcacca aagtgaatt aggagctgc tttatatcc cacaggaca cgccaccgc tcccaaaat acccagcat ccttcctcc gggtgccca acacaccca aacaggatt attcccacc gcctagcta ctggccgta ttcacctgg atcgttcga tccccgcgg acccttcaa ttgatccag taacattcc agttgttag aaatgtgta tatcgagtt acgttcctt gtttttcca ataaattgt acatattgt g
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
