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Gene information
General information
TC008303 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8152054..8160818
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008303
    iBeetle screen iB_02774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection


    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    abdominal odoriferous glands potentiallycontent not present - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    mandible decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs pretarsus shape broader - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008303 RA
      MSEIKRASL IPLGPQKES DKSSKEPRK TVRISVVLP 
      ESTEDTCPE FNYKDELAA AKKRLKYQE MGGTANGLS 
      KFDEFDDDD DVKRIARQM EEKYGTGTS LNKKKRKGR 
      KDDYADIGM GYDESDSFI DNTDGYDEM IPQNVTTLH 
      GGFYINSGA LEFKTDDEA ESDFSSSSS DDEGSRKTA 
      SRKRIIESS DETDRENKT ETVDNPSTV PKKRKIQNG 
      GNGVQQSLK KKLLAQNKI YIKKRRLLD PDKKTVKEL 
      LREKREDLN MSIPEELKL NDSINEDQK DKKNMSISN 
      VTDVIESVV KAATGPETN QNTPTKVVA NSTSDGESS 
      HDNTREVKL PENLSPDIA SVITAIKRS SELNTDPNN 
      IFTSEINIL ILRLERKCK CLGKPSKMK VYEHLATFL 
      KCKTETLTR RAKSLVLDD EQKKLKNLL SGLKSEINN 
      IMPSLLESY EKESQRIIQ KKFSHENAD NEDNKSLRL 
      PKRRFQWNE VTKKLLKDI LILKKRSLL FEGKTKESL 
      DTQMTTFLK TEIQVLWPE GWMSMNALN KIYTTYADP 
      KRLINSSLS SNSPNSTPP TKPSSSSNS TPLVKNLPQ 
      SFNNSNLSI TPIVPAAKS ETSGNKILS VTNEVTVSK 
      TYEPSKFTK SLPETPQPC DISPASPKK EFEGKLKVK 
      PSSELLKPP VSNHVRKDP MPISDDSVI YIDSDGFNP 
      MKKSDDNYC QVIDLSDKA EFKKEAKSV PKKETSISP 
      LIPEHVSKH HELSITPTY PKIDPKPKT SCAAGDDIQ 
      KVMENLKVL QKMSSPKKC EPAQSSSAV SVIAVNKSF 
      APKPTHEAS TMQRYDYNK SDYNTGFQD EFQKQLFSA 
      LNQISSNSN KSYNSTDRY PHISNSGPA MVSGDKYYQ 
      TANNNYPQS SNGHKSSTS VQQTGTYSI LDDMFANLL 
      TQYGYSKEA AAPKSQQK
    • >TC008303 RB
      MSEIKRASL IPLGPQKES DKSSKEPRK TVRISVVLP 
      ESTEDTCPE FNYKDELAA AKKRLKYQE MGGTANGLS 
      KFDEFDDDD DVKRIARQM EEKYGTGTS LNKKKRKGR 
      KDDYADIGM GYDESDSFI DNTDGYDEM IPQNVTTLH 
      GGFYINSGA LEFKTDDEA ESDFSSSSS DDEGSRKTA 
      SRKRIIESS DETDRENKT ETVDNPSTV PKKRKIQNG 
      GNGVQQSLK KKLLAQNKI YIKKRRLLD PDKKTVKEL 
      LREKREDLN MSIPEELKL NDSINEDQK DKKNMSISN 
      VTDVIESVV KAATGPETN QNTPTKVVA NSTSDGESS 
      HDNTREVKL PENLSPDIA SVITAIKRS SELNTDPNN 
      IFTSEINIL ILRLERKCK CLGKPSKMK VYEHLATFL 
      KCKTETLTR RAKSLVLDD EQKKLKNLL SGLKSEINN 
      IMPSLLESY EKESQRIIQ KKFSHENAD NEDNKSLRL 
      PKRRFQWNE VTKKLLKDI LILKKRSLL FEGKTKESL 
      DTQMTTFLK TEIQVLWPE GWMSMNALN KIYTTYADP 
      KRLINSSLS SNSPNSTPP TKPSSSSNS TPLVKNLPQ 
      SFNNSNLSI TPIVPAAKS ETSGNKILS VTNEVTVSK 
      TYEPSKFTK SLPETPQPC DISPASPKK EFEGKLKVK 
      PSSELLKPP VSNHVRKDP MPISDDSVI YIDSDGFNP 
      MKKSDDNYC QVIDLSDKA EFKKEAKSV PKKETSISP 
      LIPEHVSKH HELSITPTY PKIDPKPKT SCAAGDDIQ 
      KVMENLKVL QKMSSPKKC EPAQSSSAV SVIAVNKSF 
      APKPTHEAS TMQRYDYNK SDYNTGFQD EFQKQLFSA 
      LNQISSNSN KSYNSTDRY PHISNSGPA MVSGDKYYQ 
      TANNNYPQS SNGHKSSTS VQQTAAAPK SQQK
    • >TC008303 RC
      MSEIKRASL IPLGPQKES DKSSKEPRK TVRISVVLP 
      ESTEDTCPE FNYKDELAA AKKRLKYQE MGGTANGLS 
      KFDEFDDDD DVKRIARQM EEKYGTGTS LNKKKRKGR 
      KDDYADIGM GYDESDSFI DNTDGYDEM IPQNVTTLH 
      GGFYINSGA LEFKTDDEA ESDFSSSSS DDEGSRKTA 
      SRKRIIESS DETDRENKT ETVDNPSTV PKKRKIQNG 
      GNGVQQSLK KKLLAQNKI YIKKRRLLD PDKKTVKEL 
      LREKREDLN MSIPEELKL NDSINEDQK DKKNMSISN 
      VTDVIESVV KAATGPETN QNTPTKVVA NSTSDGESS 
      HDNTREVKL PENLSPDIA SVITAIKRS SELNTDPNN 
      IFTSEINIL ILRLERKCK CLGKPSKMK VYEHLATFL 
      KCKTETLTR RAKSLVLDD EQKKLKNLL SGLKSEINN 
      IMPSLLESY EKESQRIIQ KKFSHENAD NEDNKSLRL 
      PKRRFQWNE VTKKLLKDI LILKKRSLL FEGKTKESL 
      DTQMTTFLK TEIQVLWPE GWMSMNALN KIYTTYADP 
      KRLINSSLS SNSPNSTPP TKPSSSSNS TPLVKNLPQ 
      SFNNSNLSI TPIVPAAKS ETSGNKILS VTNEVTVSK 
      TYEPSKFTK SLPETPQPC DISPASPKK EFEGKLKVK 
      PSSELLKPP VSNHVRKDP MPISDDSVI YIDSDGFNP 
      MKKSDDNYC QVIDLSDKA EFKKEAKSV PKKETSISP 
      LIPEHVSKH HELSITPTY PKIDPKPKT SCAAGDDIQ 
      KVMENLKVL QKMSSPKKC EPAQSSSAV SVIAVNKSF 
      APKPTHEAS TMQRYDYNK SDYNTGFQD EFQKQLFSA 
      LNQISSNSN KSYNSTDRY PHISNSGPA MVSGDKYYQ 
      TANNNYPQS SNGHKSSTS VQQTGTYSI LDDMFANLL 
      TQYGYSKEG MRCANMSGV E
    CDS sequence
    • >TC008303 RA
      atgtcagaa ataaaacgt gcctcgctg attcctcta 
      ggaccccaa aaagagtcg gacaagtcg tcgaaggag 
      ccccgcaag accgtaagg atatccgtt gttctgccc 
      gagtccacc gaagacaca tgcccggaa ttcaactat 
      aaagatgaa cttgctgct gcaaagaaa cgcctcaaa 
      tatcaagaa atgggcggg acggcgaat ggtttgagt 
      aaatttgat gagttcgac gacgatgac gatgtcaaa 
      agaatcgct cgtcaaatg gaggaaaaa tatggcacg 
      gggacgtct ttaaataaa aagaaacgg aaaggtcga 
      aaggatgat tatgccgac atcgggatg ggttacgac 
      gaatccgat tcgtttatc gacaatacg gacggttac 
      gatgaaatg atccctcaa aatgtcacg acgctacat 
      gggggattt tacataaat agcggcgcg ttggagttt 
      aaaacggat gatgaggca gagtcggac ttttcttca 
      agttcatct gacgatgaa ggttcaaga aagacggct 
      agtcgaaaa agaattatt gaatcatca gatgagact 
      gatagagaa aataagact gagactgta gataatcca 
      agtaccgtg ccgaagaag cgtaaaata cagaacggt 
      ggaaatggc gtgcaacaa tcgcttaaa aagaaattg 
      ttggctcag aataaaatt tacataaag aagagacga 
      ttgttagat ccggacaag aaaacagtc aaggagctt 
      ctgagggag aagcgggaa gatttgaat atgtccatt 
      cctgaagag ctcaagctc aatgattcc attaatgaa 
      gatcaaaaa gataagaaa aacatgtcc atatcaaac 
      gttacagat gttattgaa tcggtagtt aaagctgca 
      actggaccc gaaaccaat caaaacacg ccaacaaaa 
      gttgttgct aattcaact tctgatgga gaatctagt 
      catgataac acacgagag gtgaagtta ccagaaaat 
      ttatctcca gacattgca agtgtgatc acagcgatt 
      aaacgatca tctgaacta aatacggac ccgaacaat 
      atattcaca tcggaaata aacattctt attttaaga 
      ttagaacgc aaatgtaaa tgtttgggt aagccttca 
      aaaatgaag gtctacgaa catttagcc acgttccta 
      aaatgcaaa actgaaaca ttaactagg cgggctaag 
      agtctagtt ttagacgat gagcagaag aaattgaaa 
      aatcttctg tcagggctg aagtcggaa attaacaat 
      attatgcca agcttgctg gagagctat gagaaagag 
      agtcagcgt attatacag aagaaattt tcgcacgaa 
      aatgccgac aatgaagat aataaaagt ttaaggctg 
      ccgaagcgg cgtttccag tggaatgaa gtaacaaag 
      aaactgctg aaggatatc ctcattttg aagaaacgt 
      agtctttta ttcgaaggg aagactaaa gagagttta 
      gacacccaa atgacgacg tttctcaaa accgagatt 
      caagtcttg tggcctgag ggctggatg tccatgaac 
      gcccttaac aaaatttac acgacctat gcggaccct 
      aaacgcctc attaatagt tctttgagt agtaattcc 
      ccgaatagc actcctcca actaaacct tcaagctca 
      agcaactcc actccgctc gttaaaaat cttccacaa 
      agctttaat aattctaat ttatcgata acaccaata 
      gtacccgct gcaaaaagc gagactagc ggtaataaa 
      attcttagt gtaacgaac gaagtgact gttagtaag 
      acttacgaa cctagtaaa tttacgaaa tcactgcca 
      gaaacgccg caaccgtgt gatatctcc ccggcttcg 
      cccaaaaaa gagttcgag ggcaagctg aaagtgaag 
      ccttcaagc gagttgctg aaaccacct gtgagcaac 
      catgtgagg aaggatccg atgccgatt agtgacgat 
      agtgttatt tatatagac agtgatggg tttaatccg 
      atgaagaaa agtgatgat aattactgc caagttata 
      gacttatct gataaagcc gagttcaag aaagaagcg 
      aagagtgtt ccaaagaag gagactagt atcagtccg 
      ttgattccc gagcacgtg tcgaaacat cacgaactg 
      agtattacc ccgacttac cccaaaatc gacccgaag 
      cccaaaacg agttgtgcc gccggcgac gatattcaa 
      aaagttatg gaaaatttg aaagttttg caaaaaatg 
      tcgtcgccg aaaaagtgc gaaccggct cagagtagt 
      tcggccgtt tccgtaatc gcagtgaat aaaagtttc 
      gcgccgaaa ccgacacac gaagccagt accatgcag 
      agatacgat tataataaa agtgattac aatacaggt 
      tttcaagac gaattccaa aaacagttg tttagtgct 
      ctcaatcag atttcgtca aattccaac aaaagctat 
      aatagtact gataggtat ccgcatatt tcaaacagt 
      gggcccgcc atggtttcc ggtgataag tactatcag 
      accgctaat aataattac cctcagagt tcaaacggt 
      cacaagagc agcacttct gtccagcaa acaggtact 
      tatagcata ttggatgat atgtttgct aatttactc 
      acacagtat ggatattca aaagaagct gcagcaccg 
      aaatctcaa caaaaatag
    • >TC008303 RB
      atgtcagaa ataaaacgt gcctcgctg attcctcta 
      ggaccccaa aaagagtcg gacaagtcg tcgaaggag 
      ccccgcaag accgtaagg atatccgtt gttctgccc 
      gagtccacc gaagacaca tgcccggaa ttcaactat 
      aaagatgaa cttgctgct gcaaagaaa cgcctcaaa 
      tatcaagaa atgggcggg acggcgaat ggtttgagt 
      aaatttgat gagttcgac gacgatgac gatgtcaaa 
      agaatcgct cgtcaaatg gaggaaaaa tatggcacg 
      gggacgtct ttaaataaa aagaaacgg aaaggtcga 
      aaggatgat tatgccgac atcgggatg ggttacgac 
      gaatccgat tcgtttatc gacaatacg gacggttac 
      gatgaaatg atccctcaa aatgtcacg acgctacat 
      gggggattt tacataaat agcggcgcg ttggagttt 
      aaaacggat gatgaggca gagtcggac ttttcttca 
      agttcatct gacgatgaa ggttcaaga aagacggct 
      agtcgaaaa agaattatt gaatcatca gatgagact 
      gatagagaa aataagact gagactgta gataatcca 
      agtaccgtg ccgaagaag cgtaaaata cagaacggt 
      ggaaatggc gtgcaacaa tcgcttaaa aagaaattg 
      ttggctcag aataaaatt tacataaag aagagacga 
      ttgttagat ccggacaag aaaacagtc aaggagctt 
      ctgagggag aagcgggaa gatttgaat atgtccatt 
      cctgaagag ctcaagctc aatgattcc attaatgaa 
      gatcaaaaa gataagaaa aacatgtcc atatcaaac 
      gttacagat gttattgaa tcggtagtt aaagctgca 
      actggaccc gaaaccaat caaaacacg ccaacaaaa 
      gttgttgct aattcaact tctgatgga gaatctagt 
      catgataac acacgagag gtgaagtta ccagaaaat 
      ttatctcca gacattgca agtgtgatc acagcgatt 
      aaacgatca tctgaacta aatacggac ccgaacaat 
      atattcaca tcggaaata aacattctt attttaaga 
      ttagaacgc aaatgtaaa tgtttgggt aagccttca 
      aaaatgaag gtctacgaa catttagcc acgttccta 
      aaatgcaaa actgaaaca ttaactagg cgggctaag 
      agtctagtt ttagacgat gagcagaag aaattgaaa 
      aatcttctg tcagggctg aagtcggaa attaacaat 
      attatgcca agcttgctg gagagctat gagaaagag 
      agtcagcgt attatacag aagaaattt tcgcacgaa 
      aatgccgac aatgaagat aataaaagt ttaaggctg 
      ccgaagcgg cgtttccag tggaatgaa gtaacaaag 
      aaactgctg aaggatatc ctcattttg aagaaacgt 
      agtctttta ttcgaaggg aagactaaa gagagttta 
      gacacccaa atgacgacg tttctcaaa accgagatt 
      caagtcttg tggcctgag ggctggatg tccatgaac 
      gcccttaac aaaatttac acgacctat gcggaccct 
      aaacgcctc attaatagt tctttgagt agtaattcc 
      ccgaatagc actcctcca actaaacct tcaagctca 
      agcaactcc actccgctc gttaaaaat cttccacaa 
      agctttaat aattctaat ttatcgata acaccaata 
      gtacccgct gcaaaaagc gagactagc ggtaataaa 
      attcttagt gtaacgaac gaagtgact gttagtaag 
      acttacgaa cctagtaaa tttacgaaa tcactgcca 
      gaaacgccg caaccgtgt gatatctcc ccggcttcg 
      cccaaaaaa gagttcgag ggcaagctg aaagtgaag 
      ccttcaagc gagttgctg aaaccacct gtgagcaac 
      catgtgagg aaggatccg atgccgatt agtgacgat 
      agtgttatt tatatagac agtgatggg tttaatccg 
      atgaagaaa agtgatgat aattactgc caagttata 
      gacttatct gataaagcc gagttcaag aaagaagcg 
      aagagtgtt ccaaagaag gagactagt atcagtccg 
      ttgattccc gagcacgtg tcgaaacat cacgaactg 
      agtattacc ccgacttac cccaaaatc gacccgaag 
      cccaaaacg agttgtgcc gccggcgac gatattcaa 
      aaagttatg gaaaatttg aaagttttg caaaaaatg 
      tcgtcgccg aaaaagtgc gaaccggct cagagtagt 
      tcggccgtt tccgtaatc gcagtgaat aaaagtttc 
      gcgccgaaa ccgacacac gaagccagt accatgcag 
      agatacgat tataataaa agtgattac aatacaggt 
      tttcaagac gaattccaa aaacagttg tttagtgct 
      ctcaatcag atttcgtca aattccaac aaaagctat 
      aatagtact gataggtat ccgcatatt tcaaacagt 
      gggcccgcc atggtttcc ggtgataag tactatcag 
      accgctaat aataattac cctcagagt tcaaacggt 
      cacaagagc agcacttct gtccagcaa acagctgca 
      gcaccgaaa tctcaacaa aaatag
    • >TC008303 RC
      atgtcagaa ataaaacgt gcctcgctg attcctcta 
      ggaccccaa aaagagtcg gacaagtcg tcgaaggag 
      ccccgcaag accgtaagg atatccgtt gttctgccc 
      gagtccacc gaagacaca tgcccggaa ttcaactat 
      aaagatgaa cttgctgct gcaaagaaa cgcctcaaa 
      tatcaagaa atgggcggg acggcgaat ggtttgagt 
      aaatttgat gagttcgac gacgatgac gatgtcaaa 
      agaatcgct cgtcaaatg gaggaaaaa tatggcacg 
      gggacgtct ttaaataaa aagaaacgg aaaggtcga 
      aaggatgat tatgccgac atcgggatg ggttacgac 
      gaatccgat tcgtttatc gacaatacg gacggttac 
      gatgaaatg atccctcaa aatgtcacg acgctacat 
      gggggattt tacataaat agcggcgcg ttggagttt 
      aaaacggat gatgaggca gagtcggac ttttcttca 
      agttcatct gacgatgaa ggttcaaga aagacggct 
      agtcgaaaa agaattatt gaatcatca gatgagact 
      gatagagaa aataagact gagactgta gataatcca 
      agtaccgtg ccgaagaag cgtaaaata cagaacggt 
      ggaaatggc gtgcaacaa tcgcttaaa aagaaattg 
      ttggctcag aataaaatt tacataaag aagagacga 
      ttgttagat ccggacaag aaaacagtc aaggagctt 
      ctgagggag aagcgggaa gatttgaat atgtccatt 
      cctgaagag ctcaagctc aatgattcc attaatgaa 
      gatcaaaaa gataagaaa aacatgtcc atatcaaac 
      gttacagat gttattgaa tcggtagtt aaagctgca 
      actggaccc gaaaccaat caaaacacg ccaacaaaa 
      gttgttgct aattcaact tctgatgga gaatctagt 
      catgataac acacgagag gtgaagtta ccagaaaat 
      ttatctcca gacattgca agtgtgatc acagcgatt 
      aaacgatca tctgaacta aatacggac ccgaacaat 
      atattcaca tcggaaata aacattctt attttaaga 
      ttagaacgc aaatgtaaa tgtttgggt aagccttca 
      aaaatgaag gtctacgaa catttagcc acgttccta 
      aaatgcaaa actgaaaca ttaactagg cgggctaag 
      agtctagtt ttagacgat gagcagaag aaattgaaa 
      aatcttctg tcagggctg aagtcggaa attaacaat 
      attatgcca agcttgctg gagagctat gagaaagag 
      agtcagcgt attatacag aagaaattt tcgcacgaa 
      aatgccgac aatgaagat aataaaagt ttaaggctg 
      ccgaagcgg cgtttccag tggaatgaa gtaacaaag 
      aaactgctg aaggatatc ctcattttg aagaaacgt 
      agtctttta ttcgaaggg aagactaaa gagagttta 
      gacacccaa atgacgacg tttctcaaa accgagatt 
      caagtcttg tggcctgag ggctggatg tccatgaac 
      gcccttaac aaaatttac acgacctat gcggaccct 
      aaacgcctc attaatagt tctttgagt agtaattcc 
      ccgaatagc actcctcca actaaacct tcaagctca 
      agcaactcc actccgctc gttaaaaat cttccacaa 
      agctttaat aattctaat ttatcgata acaccaata 
      gtacccgct gcaaaaagc gagactagc ggtaataaa 
      attcttagt gtaacgaac gaagtgact gttagtaag 
      acttacgaa cctagtaaa tttacgaaa tcactgcca 
      gaaacgccg caaccgtgt gatatctcc ccggcttcg 
      cccaaaaaa gagttcgag ggcaagctg aaagtgaag 
      ccttcaagc gagttgctg aaaccacct gtgagcaac 
      catgtgagg aaggatccg atgccgatt agtgacgat 
      agtgttatt tatatagac agtgatggg tttaatccg 
      atgaagaaa agtgatgat aattactgc caagttata 
      gacttatct gataaagcc gagttcaag aaagaagcg 
      aagagtgtt ccaaagaag gagactagt atcagtccg 
      ttgattccc gagcacgtg tcgaaacat cacgaactg 
      agtattacc ccgacttac cccaaaatc gacccgaag 
      cccaaaacg agttgtgcc gccggcgac gatattcaa 
      aaagttatg gaaaatttg aaagttttg caaaaaatg 
      tcgtcgccg aaaaagtgc gaaccggct cagagtagt 
      tcggccgtt tccgtaatc gcagtgaat aaaagtttc 
      gcgccgaaa ccgacacac gaagccagt accatgcag 
      agatacgat tataataaa agtgattac aatacaggt 
      tttcaagac gaattccaa aaacagttg tttagtgct 
      ctcaatcag atttcgtca aattccaac aaaagctat 
      aatagtact gataggtat ccgcatatt tcaaacagt 
      gggcccgcc atggtttcc ggtgataag tactatcag 
      accgctaat aataattac cctcagagt tcaaacggt 
      cacaagagc agcacttct gtccagcaa acaggtact 
      tatagcata ttggatgat atgtttgct aatttactc 
      acacagtat ggatattca aaagaaggt atgaggtgt 
      gcaaatatg tctggtgtt gaataa
    mRNA sequence
    • >TC008303 RA
      cgtggtttg tttgagcta gtgtgtttc aaacatgtt 
      ggagcgaaa ttgtaaagt gttgtgatg ttgtccagt 
      tagctctta tgaaataat gtcagaaat aaaacgtgc 
      ctcgctgat tcctctagg accccaaaa agagtcgga 
      caagtcgtc gaaggagcc ccgcaagac cgtaaggat 
      atccgttgt tctgcccga gtccaccga agacacatg 
      cccggaatt caactataa agatgaact tgctgctgc 
      aaagaaacg cctcaaata tcaagaaat gggcgggac 
      ggcgaatgg tttgagtaa atttgatga gttcgacga 
      cgatgacga tgtcaaaag aatcgctcg tcaaatgga 
      ggaaaaata tggcacggg gacgtcttt aaataaaaa 
      gaaacggaa aggtcgaaa ggatgatta tgccgacat 
      cgggatggg ttacgacga atccgattc gtttatcga 
      caatacgga cggttacga tgaaatgat ccctcaaaa 
      tgtcacgac gctacatgg gggatttta cataaatag 
      cggcgcgtt ggagtttaa aacggatga tgaggcaga 
      gtcggactt ttcttcaag ttcatctga cgatgaagg 
      ttcaagaaa gacggctag tcgaaaaag aattattga 
      atcatcaga tgagactga tagagaaaa taagactga 
      gactgtaga taatccaag taccgtgcc gaagaagcg 
      taaaataca gaacggtgg aaatggcgt gcaacaatc 
      gcttaaaaa gaaattgtt ggctcagaa taaaattta 
      cataaagaa gagacgatt gttagatcc ggacaagaa 
      aacagtcaa ggagcttct gagggagaa gcgggaaga 
      tttgaatat gtccattcc tgaagagct caagctcaa 
      tgattccat taatgaaga tcaaaaaga taagaaaaa 
      catgtccat atcaaacgt tacagatgt tattgaatc 
      ggtagttaa agctgcaac tggacccga aaccaatca 
      aaacacgcc aacaaaagt tgttgctaa ttcaacttc 
      tgatggaga atctagtca tgataacac acgagaggt 
      gaagttacc agaaaattt atctccaga cattgcaag 
      tgtgatcac agcgattaa acgatcatc tgaactaaa 
      tacggaccc gaacaatat attcacatc ggaaataaa 
      cattcttat tttaagatt agaacgcaa atgtaaatg 
      tttgggtaa gccttcaaa aatgaaggt ctacgaaca 
      tttagccac gttcctaaa atgcaaaac tgaaacatt 
      aactaggcg ggctaagag tctagtttt agacgatga 
      gcagaagaa attgaaaaa tcttctgtc agggctgaa 
      gtcggaaat taacaatat tatgccaag cttgctgga 
      gagctatga gaaagagag tcagcgtat tatacagaa 
      gaaattttc gcacgaaaa tgccgacaa tgaagataa 
      taaaagttt aaggctgcc gaagcggcg tttccagtg 
      gaatgaagt aacaaagaa actgctgaa ggatatcct 
      cattttgaa gaaacgtag tcttttatt cgaagggaa 
      gactaaaga gagtttaga cacccaaat gacgacgtt 
      tctcaaaac cgagattca agtcttgtg gcctgaggg 
      ctggatgtc catgaacgc ccttaacaa aatttacac 
      gacctatgc ggaccctaa acgcctcat taatagttc 
      tttgagtag taattcccc gaatagcac tcctccaac 
      taaaccttc aagctcaag caactccac tccgctcgt 
      taaaaatct tccacaaag ctttaataa ttctaattt 
      atcgataac accaatagt acccgctgc aaaaagcga 
      gactagcgg taataaaat tcttagtgt aacgaacga 
      agtgactgt tagtaagac ttacgaacc tagtaaatt 
      tacgaaatc actgccaga aacgccgca accgtgtga 
      tatctcccc ggcttcgcc caaaaaaga gttcgaggg 
      caagctgaa agtgaagcc ttcaagcga gttgctgaa 
      accacctgt gagcaacca tgtgaggaa ggatccgat 
      gccgattag tgacgatag tgttattta tatagacag 
      tgatgggtt taatccgat gaagaaaag tgatgataa 
      ttactgcca agttataga cttatctga taaagccga 
      gttcaagaa agaagcgaa gagtgttcc aaagaagga 
      gactagtat cagtccgtt gattcccga gcacgtgtc 
      gaaacatca cgaactgag tattacccc gacttaccc 
      caaaatcga cccgaagcc caaaacgag ttgtgccgc 
      cggcgacga tattcaaaa agttatgga aaatttgaa 
      agttttgca aaaaatgtc gtcgccgaa aaagtgcga 
      accggctca gagtagttc ggccgtttc cgtaatcgc 
      agtgaataa aagtttcgc gccgaaacc gacacacga 
      agccagtac catgcagag atacgatta taataaaag 
      tgattacaa tacaggttt tcaagacga attccaaaa 
      acagttgtt tagtgctct caatcagat ttcgtcaaa 
      ttccaacaa aagctataa tagtactga taggtatcc 
      gcatatttc aaacagtgg gcccgccat ggtttccgg 
      tgataagta ctatcagac cgctaataa taattaccc 
      tcagagttc aaacggtca caagagcag cacttctgt 
      ccagcaaac aggtactta tagcatatt ggatgatat 
      gtttgctaa tttactcac acagtatgg atattcaaa 
      agaagctgc agcaccgaa atctcaaca aaaatagct 
      aggggaagt cgtaactta atttatgaa gataatgta 
      ttgtactgc caactttta taaagtttt gtccttttt 
      cattttgta gaaagatta tagattcgt cttttgtta 
      ttattatta attttgtaa tttattttc attgtaaat 
      ttgtaataa attgtgatt tctgaaata tatttttta 
      ccgagtgcg agcttttat ttcaaacct accgcccag 
      cagtgttgc tcccagcct cgaactccc taaaccgag 
      cagttaagc agaaaatta aagctacat ttgcatata 
      acgggagct taatttgct actagacaa gaaataaat 
      aataatcaa ataaaaaaa ctgtaaaat tattaaaat 
      cggtggtag aggtgtggt ttggaagag gcttggcct 
      cccactttt ttgacaatc gaaccattt ctaccaact 
      ctcgattca ctcagtgtc tacttcggt tgtagagag 
      gttaataac accaataaa ttattttat tgagtgagt 
      actgtgctc aaattttca tattcgtat tgattttcg 
      tagtttcta gttagaaaa agtagtgga tattgctac 
      aactgcatg ggaggtatt agggctaag cgctgctct 
      ccattctgc agggggagg taagtcgat actcacaga 
      gctttattt
    • >TC008303 RB
      cgtggtttg tttgagcta gtgtgtttc aaacatgtt 
      ggagcgaaa ttgtaaagt gttgtgatg ttgtccagt 
      tagctctta tgaaataat gtcagaaat aaaacgtgc 
      ctcgctgat tcctctagg accccaaaa agagtcgga 
      caagtcgtc gaaggagcc ccgcaagac cgtaaggat 
      atccgttgt tctgcccga gtccaccga agacacatg 
      cccggaatt caactataa agatgaact tgctgctgc 
      aaagaaacg cctcaaata tcaagaaat gggcgggac 
      ggcgaatgg tttgagtaa atttgatga gttcgacga 
      cgatgacga tgtcaaaag aatcgctcg tcaaatgga 
      ggaaaaata tggcacggg gacgtcttt aaataaaaa 
      gaaacggaa aggtcgaaa ggatgatta tgccgacat 
      cgggatggg ttacgacga atccgattc gtttatcga 
      caatacgga cggttacga tgaaatgat ccctcaaaa 
      tgtcacgac gctacatgg gggatttta cataaatag 
      cggcgcgtt ggagtttaa aacggatga tgaggcaga 
      gtcggactt ttcttcaag ttcatctga cgatgaagg 
      ttcaagaaa gacggctag tcgaaaaag aattattga 
      atcatcaga tgagactga tagagaaaa taagactga 
      gactgtaga taatccaag taccgtgcc gaagaagcg 
      taaaataca gaacggtgg aaatggcgt gcaacaatc 
      gcttaaaaa gaaattgtt ggctcagaa taaaattta 
      cataaagaa gagacgatt gttagatcc ggacaagaa 
      aacagtcaa ggagcttct gagggagaa gcgggaaga 
      tttgaatat gtccattcc tgaagagct caagctcaa 
      tgattccat taatgaaga tcaaaaaga taagaaaaa 
      catgtccat atcaaacgt tacagatgt tattgaatc 
      ggtagttaa agctgcaac tggacccga aaccaatca 
      aaacacgcc aacaaaagt tgttgctaa ttcaacttc 
      tgatggaga atctagtca tgataacac acgagaggt 
      gaagttacc agaaaattt atctccaga cattgcaag 
      tgtgatcac agcgattaa acgatcatc tgaactaaa 
      tacggaccc gaacaatat attcacatc ggaaataaa 
      cattcttat tttaagatt agaacgcaa atgtaaatg 
      tttgggtaa gccttcaaa aatgaaggt ctacgaaca 
      tttagccac gttcctaaa atgcaaaac tgaaacatt 
      aactaggcg ggctaagag tctagtttt agacgatga 
      gcagaagaa attgaaaaa tcttctgtc agggctgaa 
      gtcggaaat taacaatat tatgccaag cttgctgga 
      gagctatga gaaagagag tcagcgtat tatacagaa 
      gaaattttc gcacgaaaa tgccgacaa tgaagataa 
      taaaagttt aaggctgcc gaagcggcg tttccagtg 
      gaatgaagt aacaaagaa actgctgaa ggatatcct 
      cattttgaa gaaacgtag tcttttatt cgaagggaa 
      gactaaaga gagtttaga cacccaaat gacgacgtt 
      tctcaaaac cgagattca agtcttgtg gcctgaggg 
      ctggatgtc catgaacgc ccttaacaa aatttacac 
      gacctatgc ggaccctaa acgcctcat taatagttc 
      tttgagtag taattcccc gaatagcac tcctccaac 
      taaaccttc aagctcaag caactccac tccgctcgt 
      taaaaatct tccacaaag ctttaataa ttctaattt 
      atcgataac accaatagt acccgctgc aaaaagcga 
      gactagcgg taataaaat tcttagtgt aacgaacga 
      agtgactgt tagtaagac ttacgaacc tagtaaatt 
      tacgaaatc actgccaga aacgccgca accgtgtga 
      tatctcccc ggcttcgcc caaaaaaga gttcgaggg 
      caagctgaa agtgaagcc ttcaagcga gttgctgaa 
      accacctgt gagcaacca tgtgaggaa ggatccgat 
      gccgattag tgacgatag tgttattta tatagacag 
      tgatgggtt taatccgat gaagaaaag tgatgataa 
      ttactgcca agttataga cttatctga taaagccga 
      gttcaagaa agaagcgaa gagtgttcc aaagaagga 
      gactagtat cagtccgtt gattcccga gcacgtgtc 
      gaaacatca cgaactgag tattacccc gacttaccc 
      caaaatcga cccgaagcc caaaacgag ttgtgccgc 
      cggcgacga tattcaaaa agttatgga aaatttgaa 
      agttttgca aaaaatgtc gtcgccgaa aaagtgcga 
      accggctca gagtagttc ggccgtttc cgtaatcgc 
      agtgaataa aagtttcgc gccgaaacc gacacacga 
      agccagtac catgcagag atacgatta taataaaag 
      tgattacaa tacaggttt tcaagacga attccaaaa 
      acagttgtt tagtgctct caatcagat ttcgtcaaa 
      ttccaacaa aagctataa tagtactga taggtatcc 
      gcatatttc aaacagtgg gcccgccat ggtttccgg 
      tgataagta ctatcagac cgctaataa taattaccc 
      tcagagttc aaacggtca caagagcag cacttctgt 
      ccagcaaac agctgcagc accgaaatc tcaacaaaa 
      atagctagg ggaagtcgt aacttaatt tatgaagat 
      aatgtattg tactgccaa cttttataa agttttgtc 
      ctttttcat tttgtagaa agattatag attcgtctt 
      ttgttatta ttattaatt ttgtaattt attttcatt 
      gtaaatttg taataaatt gtgatttct gaaatatat 
      tttttaccg agtgcgagc ttttatttc aaacctacc 
      gcccagcag tgttgctcc cagcctcga actccctaa 
      accgagcag ttaagcaga aaattaaag ctacatttg 
      catataacg ggagcttaa tttgctact agacaagaa 
      ataaataat aatcaaata aaaaaactg taaaattat 
      taaaatcgg tggtagagg tgtggtttg gaagaggct 
      tggcctccc acttttttg acaatcgaa ccatttcta 
      ccaactctc gattcactc agtgtctac ttcggttgt 
      agagaggtt aataacacc aataaatta ttttattga 
      gtgagtact gtgctcaaa ttttcatat tcgtattga 
      ttttcgtag tttctagtt agaaaaagt agtggatat 
      tgctacaac tgcatggga ggtattagg gctaagcgc 
      tgctctcca ttctgcagg gggaggtaa gtcgatact 
      cacagagct ttattt
    • >TC008303 RC
      cgtggtttg tttgagcta gtgtgtttc aaacatgtt 
      ggagcgaaa ttgtaaagt gttgtgatg ttgtccagt 
      tagctctta tgaaataat gtcagaaat aaaacgtgc 
      ctcgctgat tcctctagg accccaaaa agagtcgga 
      caagtcgtc gaaggagcc ccgcaagac cgtaaggat 
      atccgttgt tctgcccga gtccaccga agacacatg 
      cccggaatt caactataa agatgaact tgctgctgc 
      aaagaaacg cctcaaata tcaagaaat gggcgggac 
      ggcgaatgg tttgagtaa atttgatga gttcgacga 
      cgatgacga tgtcaaaag aatcgctcg tcaaatgga 
      ggaaaaata tggcacggg gacgtcttt aaataaaaa 
      gaaacggaa aggtcgaaa ggatgatta tgccgacat 
      cgggatggg ttacgacga atccgattc gtttatcga 
      caatacgga cggttacga tgaaatgat ccctcaaaa 
      tgtcacgac gctacatgg gggatttta cataaatag 
      cggcgcgtt ggagtttaa aacggatga tgaggcaga 
      gtcggactt ttcttcaag ttcatctga cgatgaagg 
      ttcaagaaa gacggctag tcgaaaaag aattattga 
      atcatcaga tgagactga tagagaaaa taagactga 
      gactgtaga taatccaag taccgtgcc gaagaagcg 
      taaaataca gaacggtgg aaatggcgt gcaacaatc 
      gcttaaaaa gaaattgtt ggctcagaa taaaattta 
      cataaagaa gagacgatt gttagatcc ggacaagaa 
      aacagtcaa ggagcttct gagggagaa gcgggaaga 
      tttgaatat gtccattcc tgaagagct caagctcaa 
      tgattccat taatgaaga tcaaaaaga taagaaaaa 
      catgtccat atcaaacgt tacagatgt tattgaatc 
      ggtagttaa agctgcaac tggacccga aaccaatca 
      aaacacgcc aacaaaagt tgttgctaa ttcaacttc 
      tgatggaga atctagtca tgataacac acgagaggt 
      gaagttacc agaaaattt atctccaga cattgcaag 
      tgtgatcac agcgattaa acgatcatc tgaactaaa 
      tacggaccc gaacaatat attcacatc ggaaataaa 
      cattcttat tttaagatt agaacgcaa atgtaaatg 
      tttgggtaa gccttcaaa aatgaaggt ctacgaaca 
      tttagccac gttcctaaa atgcaaaac tgaaacatt 
      aactaggcg ggctaagag tctagtttt agacgatga 
      gcagaagaa attgaaaaa tcttctgtc agggctgaa 
      gtcggaaat taacaatat tatgccaag cttgctgga 
      gagctatga gaaagagag tcagcgtat tatacagaa 
      gaaattttc gcacgaaaa tgccgacaa tgaagataa 
      taaaagttt aaggctgcc gaagcggcg tttccagtg 
      gaatgaagt aacaaagaa actgctgaa ggatatcct 
      cattttgaa gaaacgtag tcttttatt cgaagggaa 
      gactaaaga gagtttaga cacccaaat gacgacgtt 
      tctcaaaac cgagattca agtcttgtg gcctgaggg 
      ctggatgtc catgaacgc ccttaacaa aatttacac 
      gacctatgc ggaccctaa acgcctcat taatagttc 
      tttgagtag taattcccc gaatagcac tcctccaac 
      taaaccttc aagctcaag caactccac tccgctcgt 
      taaaaatct tccacaaag ctttaataa ttctaattt 
      atcgataac accaatagt acccgctgc aaaaagcga 
      gactagcgg taataaaat tcttagtgt aacgaacga 
      agtgactgt tagtaagac ttacgaacc tagtaaatt 
      tacgaaatc actgccaga aacgccgca accgtgtga 
      tatctcccc ggcttcgcc caaaaaaga gttcgaggg 
      caagctgaa agtgaagcc ttcaagcga gttgctgaa 
      accacctgt gagcaacca tgtgaggaa ggatccgat 
      gccgattag tgacgatag tgttattta tatagacag 
      tgatgggtt taatccgat gaagaaaag tgatgataa 
      ttactgcca agttataga cttatctga taaagccga 
      gttcaagaa agaagcgaa gagtgttcc aaagaagga 
      gactagtat cagtccgtt gattcccga gcacgtgtc 
      gaaacatca cgaactgag tattacccc gacttaccc 
      caaaatcga cccgaagcc caaaacgag ttgtgccgc 
      cggcgacga tattcaaaa agttatgga aaatttgaa 
      agttttgca aaaaatgtc gtcgccgaa aaagtgcga 
      accggctca gagtagttc ggccgtttc cgtaatcgc 
      agtgaataa aagtttcgc gccgaaacc gacacacga 
      agccagtac catgcagag atacgatta taataaaag 
      tgattacaa tacaggttt tcaagacga attccaaaa 
      acagttgtt tagtgctct caatcagat ttcgtcaaa 
      ttccaacaa aagctataa tagtactga taggtatcc 
      gcatatttc aaacagtgg gcccgccat ggtttccgg 
      tgataagta ctatcagac cgctaataa taattaccc 
      tcagagttc aaacggtca caagagcag cacttctgt 
      ccagcaaac aggtactta tagcatatt ggatgatat 
      gtttgctaa tttactcac acagtatgg atattcaaa 
      agaaggtat gaggtgtgc aaatatgtc tggtgttga 
      ataagattg ggcacaaat gataagtac ttattgtgc 
      ccgatttgt agcaactta cggtgattg taatccgaa 
      agttttttg ataactagt taattagta gttaatttt 
      tgttttaag atcagagac cgtggcgta attaaaacc 
      gtttggtga aagcgttga aaaatgtac cgataatta 
      actggttgt tggcatttg ggaatccct tgcatccct 
      aacgtccta acgctgctt ttctttttt gtcttcttg 
      tgactaaaa ctgtgctct gtagattct agtttgttg 
      ctcaattct tgattttgg gttggaact tgagccaac 
      taaagccaa gtaattgtg tgagtagct cagaggatt 
      aggctcttc ttttatgct tttatttgc actccttta 
      ccttctgaa ttgatccga taccattgt aaatacgtt 
      ctcgtttgc agctgcagc accgaaatc tcaacaaaa 
      atagctagg ggaagtcgt aacttaatt tatgaagat 
      aatgtattg tactgccaa cttttataa agttttgtc 
      ctttttcat tttgtagaa agattatag attcgtctt 
      ttgttatta ttattaatt ttgtaattt attttcatt 
      gtaaatttg taataaatt gtgatttct gaaatatat 
      tttttaccg agtgcgagc ttttatttc aaacctacc 
      gcccagcag tgttgctcc cagcctcga actccctaa 
      accgagcag ttaagcaga aaattaaag ctacatttg 
      catataacg ggagcttaa tttgctact agacaagaa 
      ataaataat aatcaaata aaaaaactg taaaattat 
      taaaatcgg tggtagagg tgtggtttg gaagaggct 
      tggcctccc acttttttg acaatcgaa ccatttcta 
      ccaactctc gattcactc agtgtctac ttcggttgt 
      agagaggtt aataacacc aataaatta ttttattga 
      gtgagtact gtgctcaaa ttttcatat tcgtattga 
      ttttcgtag tttctagtt agaaaaagt agtggatat 
      tgctacaac tgcatggga ggtattagg gctaagcgc 
      tgctctcca ttctgcagg gggaggtaa gtcgatact 
      cacag
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