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Gene information
General information
TC007191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 7861384..7866683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007191
    iBeetle screen iB_02772
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007191 RA
      MSSDDKKLG ASDPKDTGT GPRIRTQSE CSVTSDDGA 
      QSSSGKRQR TTSAQFMVS SGLTSASPP KFVSLEEIM 
      QAANGMRDM ALVHQIVVD DDFKLQKVE PEPNTLHKM 
      VNDTMRKAF WDLLRNELA EDPPNYTQA LVLLEDIKK 
      GLFAVLLPQ HTKIKQQIS EILDSELIK QQAENGALD 
      FGHYAHYVI SVMAKLCAP VRDEKIKEL TQTTDVIDT 
      FKGILETLD LMKLDMANF TLQMVRPDI IAHSVELER 
      KKFADFLAV QSDGLERTK KWLLKHVDT SEVPPANSA 
      SYDSFVKTA VKKASWDAF IDLMDWDEN EPYPETFLI 
      DEARLRDLQ VKTNRLTAI GTILLVTLS HAGPDLQSI 
      AEFKASLKD HISILLQSI QNDKDLKQV LPNVAEQVI 
      CDVKEAQKK YNFSGMNDV SETVLRQQI EQISNEEHK 
      IRSLVRQRI KEFFMDIIE SSTAAPQKV PTGLSSLQR 
      ELAAIAGQF LRIVSHNNT VFCIYYYDI VAAALPKPA
    CDS sequence
    • >TC007191 RA
      atgagcagc gacgataaa aaactgggg gcttcggac 
      cccaaagac accgggact gggccccga attcggacg 
      cagagcgag tgttcggtg acaagcgac gatggggcc 
      caatctagc tcaggcaaa cggcaaagg acgacgtct 
      gcccagttc atggtctcc tcgggcctg acatcggcg 
      tcgccccct aaattcgtg tctctggaa gagataatg 
      caggcagca aatgggatg cgagacatg gccctggtg 
      caccaaatc gtcgttgac gacgatttc aagttgcaa 
      aaagttgag cctgagccc aacactttg cacaaaatg 
      gtaaatgat accatgcgt aaggccttt tgggacttg 
      ctccggaac gaactcgcc gaagatccg ccgaattac 
      acgcaggcc ctggttctt ttggaggat atcaagaaa 
      ggattattt gcagtcctg cttccgcaa cacacaaaa 
      atcaagcaa caaatttcc gagattttg gacagcgaa 
      ctgattaag caacaggcg gaaaatggg gccttggac 
      tttgggcat tatgcccac tacgtaatt tctgtgatg 
      gccaaatta tgtgcacca gtcagggac gaaaaaatc 
      aaggagctg acacagaca acggatgtc attgatacg 
      ttcaagggg atattagag actctagat ctaatgaag 
      ttggatatg gcgaatttc accttacaa atggttcgt 
      cctgacata atcgcgcac agcgtggaa ttggagcgc 
      aaaaagttc gccgatttc ctggccgtg caaagcgac 
      ggtctcgag cgcaccaag aagtggctt ctgaagcac 
      gtggacacc tcagaagtg ccccctgcc aactcggct 
      tcgtacgat tcgttcgta aaaacggcc gtgaaaaaa 
      gcatcctgg gacgctttc attgatttg atggactgg 
      gacgagaac gagccttat ccggagact ttcttgatc 
      gatgaggcg cgcttgagg gacttgcaa gtgaaaacg 
      aatcgattg accgcaatc gggaccatt ttactggtg 
      actttaagc catgcaggt ccggacttg caatcaatt 
      gcagagttt aaagcttcc ctcaaagac cacatttca 
      attctgctc caatctatt caaaatgac aaggatctg 
      aaacaagtc ttgccgaat gtggccgag caggtgatc 
      tgtgatgtt aaagaggcg cagaagaag tacaatttt 
      agcgggatg aatgatgtg agtgagacg gttttgagg 
      cagcaaatt gaacaaatc agcaatgag gagcacaag 
      atacgatca ctagttcgt caacggatt aaggaattt 
      ttcatggat ataatcgag tcttcgacg gcagcgccg 
      caaaaagtg cctacagga ttgagttct cttcaaagg 
      gagttggca gctattgca gggcaattt ctaaggatc 
      gtctcacac aataacacc gttttctgc atttactat 
      tacgacatt gtagcggca gcattacct aaaccagct 
      taa
    mRNA sequence
    • >TC007191 RA
      gtcttttgt tgttggtaa acattgaac agctgtgtt 
      tttgacgtt ttttggaga attttcgaa attttcgtg 
      tctttttca aaaaaatta gctgatccg caatgagca 
      gcgacgata aaaaactgg gggcttcgg accccaaag 
      acaccggga ctgggcccc gaattcgga cgcagagcg 
      agtgttcgg tgacaagcg acgatgggg cccaatcta 
      gctcaggca aacggcaaa ggacgacgt ctgcccagt 
      tcatggtct cctcgggcc tgacatcgg cgtcgcccc 
      ctaaattcg tgtctctgg aagagataa tgcaggcag 
      caaatggga tgcgagaca tggccctgg tgcaccaaa 
      tcgtcgttg acgacgatt tcaagttgc aaaaagttg 
      agcctgagc ccaacactt tgcacaaaa tggtaaatg 
      ataccatgc gtaaggcct tttgggact tgctccgga 
      acgaactcg ccgaagatc cgccgaatt acacgcagg 
      ccctggttc ttttggagg atatcaaga aaggattat 
      ttgcagtcc tgcttccgc aacacacaa aaatcaagc 
      aacaaattt ccgagattt tggacagcg aactgatta 
      agcaacagg cggaaaatg gggccttgg actttgggc 
      attatgccc actacgtaa tttctgtga tggccaaat 
      tatgtgcac cagtcaggg acgaaaaaa tcaaggagc 
      tgacacaga caacggatg tcattgata cgttcaagg 
      ggatattag agactctag atctaatga agttggata 
      tggcgaatt tcaccttac aaatggttc gtcctgaca 
      taatcgcgc acagcgtgg aattggagc gcaaaaagt 
      tcgccgatt tcctggccg tgcaaagcg acggtctcg 
      agcgcacca agaagtggc ttctgaagc acgtggaca 
      cctcagaag tgccccctg ccaactcgg cttcgtacg 
      attcgttcg taaaaacgg ccgtgaaaa aagcatcct 
      gggacgctt tcattgatt tgatggact gggacgaga 
      acgagcctt atccggaga ctttcttga tcgatgagg 
      cgcgcttga gggacttgc aagtgaaaa cgaatcgat 
      tgaccgcaa tcgggacca ttttactgg tgactttaa 
      gccatgcag gtccggact tgcaatcaa ttgcagagt 
      ttaaagctt ccctcaaag accacattt caattctgc 
      tccaatcta ttcaaaatg acaaggatc tgaaacaag 
      tcttgccga atgtggccg agcaggtga tctgtgatg 
      ttaaagagg cgcagaaga agtacaatt ttagcggga 
      tgaatgatg tgagtgaga cggttttga ggcagcaaa 
      ttgaacaaa tcagcaatg aggagcaca agatacgat 
      cactagttc gtcaacgga ttaaggaat ttttcatgg 
      atataatcg agtcttcga cggcagcgc cgcaaaaag 
      tgcctacag gattgagtt ctcttcaaa gggagttgg 
      cagctattg cagggcaat ttctaagga tcgtctcac 
      acaataaca ccgttttct gcatttact attacgaca 
      ttgtagcgg cagcattac ctaaaccag cttaattaa 
      accaatttc gcattctct atttcaatt tttttagga 
      ttaatttac ttaaactga gtggaaact gttgtgata 
      cgtttgtag tcaactcag ccacaaagt ggtagtggg 
      gttttgttc taaaagcgc aaatctact aaccaagta 
      gccaaaaag ttcattatt tgtatgtga tactgacct 
      ttgtagcaa gctttcgaa taaatttct ggttactgt 
      tttaagttg caaattcaa cgtcaatgt tgccaactt 
      aatttcata accacaggt cattttaat cagaaattt 
      atttacaag cctctggtg tatctaaaa ccgtagact 
      agatttttt aacaagtaa aagcctgtg aacgcaacg 
      cagtaaata aattattat tccaaagct gttttttct 
      ttaataaag acgtaaaag gttggcttt aaattatca 
      cataaaaag cttttatta ccgactata attgttctt 
      ttcacatta ctttactca atcagtttt atataacta 
      attaaaaaa tgcttacat tcacacgtt ttttattac 
      gttttaata taatcttgt tacagtctt ttaatatgt 
      agctggttc ctggctgta tgggaccca ccataaaca 
      cttaatttt accagaact tccaagtct agtgtgtag 
      agcattgtt cacatttat ttacgcaaa aactgaaaa 
      aatcgtaaa caaacacaa tcattttac acaaaaatt 
      ttacataat gtatacagc acctacgac atttacacg 
      ctttaatcc taagcaaaa acatttgta ttgcaaatt 
      tttaataaa aactattgc gaaaa